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hTERT inhibition by Retinoic Acid derivatives in MCF-7 and T47D breast cancer cells.

Breast cancer, one of the leading causes of cancer-related death in women, has presented limited treatment options due to several factors including associated toxicity and a lack of sustained drug potency. Retinoid-induced inhibition of the telomerase enzyme has offered new hope in potential treatment of breast tumors with a decreased likelihood of adverse side effects. This enzyme, which maintains the telomeric ends of chromosomes, is silenced in almost all normal somatic tissue but is active in up to 95% of neoplastic tissue, including breast tumors. Retinoic acids have been shown in previous cancer studies to effectively enact a down-regulation of transcription of hTERT, the catalytic subunit of telomerase, associated with a marked decrease in telomerase activity as well as growth inhibition of transformed cells. In this study, MCF-7 and T47D human breast cancer cells were treated with therapeutic doses of 9-cis and UAB-30 retinoic acids (RA). UAB-30 RA, specifically, is a synthetic compound proven to be effective in animal studies of the treatment of breast cancer, but to this point has yet to be tested in human models. Following treatment, RT-PCR, TRAP assay, and AlamarBlue were utilized to assess hTERT mRNA levels, telomerase activity, and cell proliferation, respectively.

Rebecca C. Wylie, Nathaniel J. Hansen, Jonathan D. Matlock, James H. Jerkins, Mitchell S. Pate, Lucy G. Andrews and Trygve O. Tollefsbol. Dept. of Biology, Univ. of Ala. at Birmingham, AL 35294.
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Title Annotation:Biological Sciences
Author:Wylie, Rebecca C.; Hansen, Nathaniel J.; Matlock, Jonathan D.; Jerkins, James H.; Pate, Mitchell S.;
Publication:Journal of the Alabama Academy of Science
Article Type:Brief Article
Date:Apr 1, 2003
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