Evaluation of four apicultural products for hive colonization by honey bees (Apis mellifera adansonii) (Hymenoptera: Apidae) and pre-colonization pests.
The African honeybee (Apis mellifera adansonii) is kept mainly for pollination of crops and its diverse products which include honey, pollen, propolis, bee venom, royal jelly and slum gum. The potential use of honey bee slum gum as an ingredient for broiler chicken feed has previously been reported . The ability to increase the supply of the bee hive products depends on several factors including hive that houses honey bee and its products and products used to attract bee colony into the hives . A major problem of beekeepers in developing countries is late colonization of hive. Several beginners were frustrated when their baited hives could not be colonized by bees despite the usage of baiting materials that were recommended by experienced beekeepers within their localities. There have been several studies on different apicultural products as colony attractant in the tropics, for instance, the use of old raw combs to attract bee colony in Himachal, Himalaya . The effectiveness of propolis, wax and cassava powder was compared in the North-western agro-ecological zone of Uganda and it was discovered that propolis performed better than the other tested attractants in term of percentage hive colonization . The use of honey as colony attractant in Nigeria was reported by Aina and Owonibi . Other researchers [1, 2, 6, 7] reported the use of bee wax for baiting of hives, as a popular baiting material in Nigeria. It is worth noting that research that attempted to compare different apicultural products as baiting materials are relatively scarce. Apicultural pests have been identified as one of the major biotic factors affecting successful beekeeping in different parts of the world [6, 8, 9, 10, 11, 12]. These pests could be categorised as pre-colonization or post-colonization pest, depending on the status of installed hives when the pest incidence occurred. It is opined that the baiting materials can influence the type and intensity of apicultural pests in an area, but this has not been established through an empirical study. Although, there have been some documented studies on performance of baits of different origin as attractants of bee colony into hives, there is paucity of information on comparison of other apicultural products with bee wax as colony attractants, at least in tropical Africa [4, 13]. To a very large extent, rural beekeepers in the tropics have traditionally used bee wax as colony attractant more than they used other apicultural products [13, 14, 15, 16, 17]. The objective of this study was to evaluate the effectiveness of four different apicultural products obtained from the same source on hive colonisation, honey yield and precolonization insect pest infestation.
MATERIALS AND METHODS
The research was carried out at Ladoke Akintola University of Technology (LAUTECH), Ventures Apiary, Ogbomoso, Nigeria. The region's climate is non-humid tropical and falls within Southern Guinea Savanna of Nigeria (8.08[degrees]N; 4.14[degrees]E at an altitude of 300 m above sea level) with a rainfall of 1400 mm, characterized by bi-modal rainfall with peaks in July and September and marked with dry and wet seasons. The apiary was on an acre of land with conservation of flora sources. The species of the flora present at the experimental site included Parkia biglobosa, Mangifera indica, Glyricidia serpeum, Aspillia africana, Azadirachta indica, Anacardium occidentalis and Pennisetum purpureum. Arable crops within the location included cassava, yam, maize, pea nut and cowpea. Their management practices were traditional without the use of inorganic fertilizer or synthetic pesticides. The tree crops and the evergreen plants in the location, besides supplying apicultural resources to the bees equally provided sufficient shade that prevented the hive from solar radiation but enabled sunshine for apicultural activities. The ecological characteristics in the experimental site favoured active foraging activities of honeybees . Water source was available within a 200-m radius to the site. The site was reasonably flat, well drained and protected from hazardous natural wind which could blow off the hives.
Hives made of Gmelina arborea L. wood were constructed according to standard model . The four sides of the hives were covered with wood given the flight entrance at the front elevation with a wooden landing board at the base and wooden top bars arranged parallel to each other on top of the hive.
Baiting and installation of hive
The hives were baited and installed on the 29th December, 2011. Prior to hive baiting, initial weight of each hive was determined with the aid of top loading weighing balance (Camry[R] Empersas, China). Four apicultural products (propolis, wax, slum gum and honey) obtained from the same apiary were used for the experiment. Ten grammes each of propolis, bee wax and slum gum and 10 ml of honey were applied at the flight entrance, walls of the hives and on the top bars. Honey was applied with the aid of a syringe (Menojet[R], China). Twelve baited hives were installed on rectangular iron stand and corrugated asbestos used as roofs [6, 7]. The experiment was replicated three times and arranged in a completely randomized design.
Incidence of pre-colonization pests, development of bee colony and harvesting of matured honey
The type and number of pre-colonization pests encountered in installed hives were recorded daily from one day post hive installation till hive colonization. Thereafter, the number of colonized hives was recorded per treatment. Weight gain of each colonized hive was taken on weekly basis with the same sensitive top-loading weighing balance used at hive installation, by subtracting the final weight from initial weight. Average weight gain was determined as total weight gain divided by number of hives that cumulated to the total weight. The accumulation of matured honey in the bee hives was monitored through visual inspection. Harvesting and processing was done according to the standard .
Data were collected on the following:
(a) Weight of matured combs harvested from each colonized hive
(b) Weight of extracted honey from the harvested combs. Thereafter, percentage honey yield was determined as:
Percentage honey yield = [Weight of extracted honey from matured combs x 100]/Weight of matured combs harvested from the hive.
The data were analyzed using the analysis of variance (ANOVA) and where there was a significant treatment difference, means were separated using Tukeys' HSD at 5% probability level, with the aid of SPSS Software .
Effect of different baiting materials on hive colonization and bee colony weight gain and yield
The effect of different baiting materials on colony weight gain was not significant (P > 0.05). Weight gain in all the treated hives increased with time and reached the peak at 16 weeks after baiting, with bee wax-baited hives having the highest weight gain (kg). This was followed by slum gum while propolis had the least weight gain (Figure 1). The first baiting material to cause hive colonization was slum gum (10 days post hive installation) (10 DPI). This was followed by bee wax (26.5 DPI) while propolis-bated hive took longest to colonization. However, bee wax had the highest percentage hive colonization (66.67%) which was not significantly (P > 0.05) different from 33.33% observed in slum gum, honey and propolis (Figure 2). Slum gum however had the highest total and honey yield (1.1kg and 0.7 kg respectively). This was followed by bee wax and honey-baited hives while the least yield was observed in propolis-baited hives (0.1 kg and 0.05 kg respectively) (Figure 3). Percentage honey yield was highest in hives baited with slum gum and lowest in propolis-baited hives (Figure 4).
Effect of different baiting materials on pest profile of baited hives
The two pests encountered at pre-colonization stage were waiver ant (Oecophylla longinuda) and sugar ant (Camponotus consobrinus). O. longinuda was significantly (P < 0.05) highest in honey treated hives (17.33) compared to other baiting materials at 2 days post hive installation (DPI) while at 3 DPI, O. longinuda had the highest population in slum gum treated hives. Slum gum and honey attracted sugar ants at 2-4 DPI while bee wax and propolis did not attract sugar ant but attracted O. longinuda at 4 DPI (Table 1).
A major problem affecting many beginner apiculturists is failure of hives to colonize. The four tested materials showed potentials to attract honey bee colony. However, higher percentage colonization was observed in wax than other apicultural products. This was consistent with the findings of previous researchers [2, 6, 7]. The reason was that the odour of wax persisted over a longer time period than other products like honey and propolis. Slum gum usually has some wax particles that can be collected with it during the rending of combs to wax. That could be the reason for its better performance than pure honey and propolis in terms of mean colonization period. In a study conducted in Nigeria, colonization rate of 50% was observed when Kenya top bar hives were baited with bee wax . However, the superior ability of propolis over bee wax as colony attractant had been reported in Uganda. Incidentally, the authors did not state possible reasons for that observation .
The result of the pest profile indicated two major pre-colonization pests viz: O. longinoda and C. consobrinus. C. pennsylvanicus had been reported as an apicultural pest in Kwara State, Nigeria . O. longinoda had been listed as a major threat to the apiculture in southwestern Nigeria . Low level of incidence of these pests in hives baited with bee wax further justified the potency of bee wax in honey bee colonization. Although the reason for low pest population in bee wax and propolis was not investigated in this study, it has been reported that worker honey bees encapsulate groups of small hive beetle (Aethina tumida) with propolis as defence mechanism . Pest control has been categorized as an important routine management known to enhance colony performance such as reduced absconding, improved colony strength and higher hive yields . It has also been noted to minimize absconding rate . Since bee wax recorded higher percentage hive colonization than other baiting materials and lower pest incidence, its outstanding candidacy as a baiting material above other studied apicultural products was established. Early colonization and high honey yield observed in slum gum made it also recommendable to farmers as a baiting material. Presently, slum gum has not been popularly used as a baiting material and has always been thrown away after rendering of wax from the combs. This study has established an aspect of its untapped benefits for the apicultural industry. Since the result of this study showed that slum gum harboured O. longinoda as a pre-colonization pest, provision should be made for the control of the pest when this product is used as honeybee colony attractant. Use of slum gum as a baiting material will reduce the cost incurred from purchase of bee wax and / or increase income realizable from the sale of bee wax: an exportable product, which has many industrial uses.
All the apicultural products used in this study attracted bee colony, but bee wax performed better, being with significantly lesser incidence of pre-colonization pests. The study established an untapped benefit of slum gum to the apicultural industry as an attractant for bee colonies into baited hives. Since slum gum is always discarded as a waste product after rendering of wax, its potential to reduce cost of production and to increase income from sale of bee wax (which is being traditionally used as baits) was established. It is, therefore, recommended for use as a baiting material in the tropical areas that share similar characteristics with the studied agro-ecological zone. However, when slum gum is to be used as colony attractant, technical means of preventing pre-colonization pests should be put in place.
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Babarinde SA (1) *, Olaleke SA (1), Akinyemi AO (2), Adebayo TA (1) and JI Olaifa (1)
* Corresponding author email: firstname.lastname@example.orgemail@example.com
(1.) Department of Crop and Environmental Protection, Ladoke Akintola University of Technology, P. M. B. 4000, Ogbomoso, Nigeria.
(2.) Department of Agronomy, Osun State University, College of Agriculture, Ejigbo Campus, P.M.B. 4494, Osogbo, Nigeria
Table 1: Occurrence of pre- colonization pests in hives baited with four different baiting materials Pest abundance 2 Days post installation Baiting Oecophylla Camponotus materials longinoda consobrinus Slum gum 4.00 [+ or -] 2.64a 132.66 [+ or -] 39.56b Bee wax 0.00 [+ or -] 0.00a 0.00 [+ or -] 0.00a Honey 17.33 [+ or -] 2.02b 105.00 [+ or -] 14.04b Propolis 0.00 [+ or -] 0.00a 0.00 [+ or -] 0.00a ANOVA [F.sub.(3,8)]=24.32 [F.sub.(3,8)]=10.97 P=0.001 P=0.001 Pest abundance 3 Days post installation Baiting Oecophylla Camponotus materials longinoda consobrinus Slum gum 12.00 [+ or -] 2.51b 40.00 [+ or -] 16.65b Bee wax 0.00 [+ or -] 0.00a 0.00 [+ or -] 0.00a Honey 4.66 [+ or -] 2.66a 34.66 [+ or -] 3.66b Propolis 0.00 [+ or -] 0.00a 0.00 [+ or -] 0.00a ANOVA [F.sub.(3,8)]=9.55 [F.sub.(3,8)]=6.45 P=0.01 P=0.01 Pest abundance 4 Days post installation Baiting Oecophylla Camponotus materials longinoda consobrinus Slum gum 3.67 [+ or -] 3.66a 23.66 [+ or -] 10.13b Bee wax 6.33 [+ or -] 3.48a 0.00 [+ or -] 0.00a Honey 14.33 [+ or -] 5.69a 33.33 [+ or -] 8.29b Propolis 5.67 [+ or -] 5.66a 0.00 [+ or -] 0.00a ANOVA [F.sub.(3,8)]=0.97; [F.sub.(3,8)]=6.67 P=0.45 P=0.01 Pest abundance 5 Days post installation Baiting Oecophylla Camponotus materials longinoda consobrinus Slum gum 0.67 [+ or -] 0.066a 2.33 [+ or -] 2.32a Bee wax 0.00 [+ or -] 0.00a 0.00 [+ or -] 0.00a Honey 0.00 [+ or -] 0.00a 4.66 [+ or -] 3.28a Propolis 0.00 [+ or -] 0.00a 0.00 [+ or -] 0.00a ANOVA [F.sub.(3,8)]=1.00; [F.sub.(3,8)]=1.23 P=0.44 P=0.36 Means followed by the same letter along the column are not significantly different using Tukeys' HSD at 5% probability level Figure 2: Effect of baiting materials on hive colonization Mean colonization Percentage period (day) colonization Slum gum 10 33.33 Honey 36 33.33 Wax 26.5 66.67 Propolis 56 33.33 Note: Table made from bar graph. Figure 3: Yield of honey bee colony in hives baited with four different baiting materials total harvest honey weight Slum gum 1.1 0.7 Honey 0.7 0.4 Wax 0.85 0.6 Propolis 0.1 0.05 Note: Table made from bar graph. Figure 4: Percentage honey yield from matured honey comb in hives baited with four different baiting materials percent yelled Slum gum 31.81 Honery 28.57 Wax 70.14 Propolis 25 Note: Table made from bar graph.