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Zinc transporter mRNA expression in the RWPE-1 human prostate epithelial cell line.

The prostate accumulates and stores the highest amount of zinc of all soft tissues within the body, yet during prostatic neoplasia, the zinc content of this organ consistently and persistently falls to much lower levels. There are specialized membrane bound transporters that help to regulate the zinc homeostasis of the prostate. These transporters have been categorized into two families: ZnT (SLC30) and Zip (SLC39). Many of these genes have been newly discovered and full function and physiological role is not yet completely known. The ZnT family is responsible for pumping zinc out of the cell or into intracellular vesicles, decreasing the intracellular levels of zinc. In contrast, the Zip family is responsible for increasing the zinc concentration by transporting the metal into the cell or out of the vesicles. The goal of the present study was to determine if the immortalized human prostate cell line (RWPE-1) could serve as a model system to study the role of zinc in prostate cancer. The study examined the expression of mRNA for 19 members of the zinc transporter gene family in normal prostate tissue, the prostate RWPE1 cell line, and the LNCaP, DU-145 and PC-3 prostate cancer cell lines. Real-time PCR was used to quantitatively measure the amount of mRNA present in normal prostate tissues, the normal prostate cell line, RWPE, and commonly utilized prostate carcinoma cell lines. The study demonstrated that the expression of the 19 zinc transporters was similar between the RWPE-1 cell line and the in situ prostate gland. Of the 19 zinc transporters, only 5 had levels that were different between the RWPE-1 cells and the tissue samples; all five being increased (ZnT-6, Zip-l, Zip-3A, Zip-10, and Zip-14). The response of the 19 transporters was also determined when the cell lines were exposed to 75 [micro]M [Zn.sup.+2] for 24h. It was shown for the RWPE-1 cells that only 5 transporters responded to [Zn.sup.+2] with mRNA for ZnT-1 and ZnT-2 being increased while mRNA for ZnT-7, Zip-7 and Zip-10 transporters were decreased. It was shown for the LNCaP, DU-145 and PC-3 cells that [Zn.sup.+2] had no effect on the mRNA levels of all 19 transporters except for an induction of ZnT-1 in PC-3 cells. Overall, the study suggests that the RWPE-1 cells could be a valuable model for the study of the zinc transporter gene family in the prostate.

Amy L. Albrecht*, Seema Somji, Mary Ann Sens, Donald A. Sens, Scott H. Garrett

University of North Dakota
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Author:Albrecht, Amy L.; Somji, Seema; Sens, Mary Ann; Sens, Donald A.; Garrett, Scott H.
Publication:Proceedings of the North Dakota Academy of Science
Article Type:Report
Geographic Code:1USA
Date:Apr 1, 2008
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