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Yersinia species isolated from bats, Germany.

To the Editor: Bats are distributed worldwide and are among the most diverse and species-rich mammals on earth. They exist in a large variety of distinct ecologic niches. Many bat species roost near humans, which is of particular interest for research on batto-human transmission of potential zoonotic pathogens. Moreover, migratory bats could act as long-distance vectors for several infectious agents. In recent decades, scientific interest in chiropteran species has markedly increased because bats are known hosts to zoonotic agents, such as henipaviruses, Ebola virus, and severe acute respiratory syndrome (SARS)-like corona viruses (1,2). However, investigations regarding bacterial pathogens with potential for mutual transmission between bats and humans are sparse. The effect of bacterial agents on individual bats is largely unknown and has been neglected in most studies published to date (3).

We conducted a broad study during 2006-2008 on diseases and causes of death in bats among 16 species found in Germany. Two hundred deceased bats, collected in different geographic regions in Germany (southern Bavaria, eastern Lower Saxony, eastern Brandenburg, and Berlin greater metropolitan area), were subjected to necropsy and investigated by using routine histopathologic and bacteriologic methods. During necropsy, instruments were dipped in 70% ethanol and moved into a Bunsen burner flame after every incision to prevent any cross-contamination. For bacteriologic examination, tissue samples were treated accordingly to prevent environmental contamination. A freshly cut tissue surface was plated onto Columbia agar (5% sheep blood; Oxoid, Wesel, Germany), Gassner agar (Oxoid), and MacConkey agar (Oxoid) and incubated at 37[degrees]C for 24-48 hours.

Twenty-five bacterial genera were cultured from bats, including 2 known human-pathogenic Yersinia spp., i.e., Y. pseudotuberculosis and Y. enterocolitica. The first Yersinia strain (Y938) was cultured from lung, heart, kidney (pure cultures), liver, spleen, and intestine (mixed cultures) of a greater mouse-eared bat (Myotis myotis). This isolate was identified as Y. pseudotuberculosis by Api 20E (bioMerieux, Nurtingen, Germany), Micronaut-E (Merlin Diagnostik GmbH, Bornheim-Hersel, Germany), and 16S rRNA gene analysis (Table). The sequence was deposited into GenBank under accession no. FN561631. Further serologic characterization by agglutination test (Denka Seiken, Tokyo, Japan) and multilocus sequence typing (4) identified Y. pseudotuberculosis serogroup 1, biovar 5, sequence type (ST) 43 in all tissue samples investigated. During necropsy, severe enlargement of the liver and a marked hemoperitoneum were seen. Microscopic examination showed multifocal severe necrotizing hepatitis and splenitis associated with numerous intralesional gram-negative coccobacilli and a moderate to marked interstitial pneumonia. The remaining organs, including heart, kidney, and intestine, had no pathologic changes.

The second Yersinia strain (Y935), Y. enterocolitica, was isolated in pure culture from spleen and intestine of a common pipistrelle (Pipistrellus pipistrellus) and identified by the methods described above (Table). The 16S rRNA sequence was deposited into GenBank under accession no. FN561632. No bacteria were cultured from any other organ. Based on results of an agglutination test (Denka Seiken), the isolate was characterized as Y. enterocolitica serotype O:6, biovar 1A. Necropsy and histopathologic examination showed no inflammatory changes, suggesting a subclinical state of infection.

Yersiniosis is a bacterial disease with a wide distribution and host range. Y. pseudotuberculosis and Y. enterocolitica are frequently isolated from a variety of wild and domestic animals (5), but little is known about the occurrence of yersiniosis in free-ranging chiropteran species. Only few reports of fatal Y. pseudotuberculosis infection in captive flying foxes have been published (6,7). In Europe, Y. pseudotuberculosis strains belonging to serogroup 1 are most common and cause most Y. pseudotuberculosis infections in humans and animals (5). Isolates of ST43 in the multilocus sequence typing database (4) came from humans, birds, hares, hedgehog, cat, dog, and pig in Europe; humans in Asia; marsupial in Australia; and deer in Australia and New Zealand. We report an isolate from a free-ranging bat in Germany. Y. enterocolitica biovar 1A has been found in a wide range of human, animal, and environmental sources. Although often considered nonpathogenic, this biovar is described as an opportunistic pathogen (8), and serovar O:6 has been detected as the causative agent of ovine placentitis and abortion (9).

Transmission of both Yersinia species generally occurs after ingestion of contaminated food or water. All bat species in Germany are insectivorous, and insects can be infected with various microbial agents. Investigations concerning bacteria-insect interactions showed that insects may carry pathogenic bacteria, including Yersinia (10); thus, insects or contaminated water are possible sources of both species described.

In conclusion, Y. pseudotuberculosis and Y. enterocolitica were isolated from 2 bat species in Germany, representing evidence of Yersinia spp. in free-ranging vespertilionids. Histopathologic findings of the greater mouse-eared bat were consistent with those of systemic Y. pseudotuberculosis infection, rendering this species pathogenic for bats. The common pipistrelle was subclinically infected with Y. enterocolitica. The role of wild animals as reservoir hosts for bacterial pathogens such as Yersinia spp. is well known, underlining the need for biologists and persons handling wildlife to be aware of these zoonotic infectious agents.


We thank Nadine Jahn, Doris Krumnow, Stephan Schatz, and Robert Schneider for excellent technical assistance and Martin Pfeffer and Holger Scholz for editorial help.

This study was supported by the Adolf and Hildegard Isler Stiftung and the Klara Samariter Stiftung. The multilocus sequence typing database is publicly available at, which is currently supported by a grant from the Science Foundation of Ireland (05/FE1/B882).

Kristin Muhldorfer, Gudrun Wibbelt, Joachim Haensel, Julia Riehm, and Stephanie Speck [1]

Author affiliations: Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany (K. Muhldorfer, G. Wibbelt, S. Speck); Berlin, (J. Haensel); and Federal Armed Forces Institute of Microbiology, Munich, Germany (J. Riehm)

DOI: 10.3201/eid1603.091035


(1.) Calisher CH, Childs JE, Field HE, Holmes KV, Schountz T. Bats: important reservoir hosts of emerging viruses. Clin Microbiol Rev. 2006;19:531-45. DOI: 10.1128/ CMR.00017-06

(2.) Wong S, Lau S, Woo P, Yuen K-Y. Bats as a continuing source of emerging infections in humans. Rev Med Virol. 2007;17:6791. DOI: 10.1002/rmv.520

(3.) Wibbelt G, Speck S, Field H. Methods for assessing diseases in bats. In: Kunz TH, Parsons S, editors. Ecological and behavioral methods for the study of bats. Baltimore: The Johns Hopkins University Press; 2009. p. 775-94

(4.) MLST Databases at the ERI. University College Cork [cited 2009 Jul 1]. http://mlst. documents/primersPseudotuberculosis_html

(5.) Mair NS. Yersiniosis in wildlife and its public health implications. J Wildl Dis. 1973;9:64-71.

(6.) Williams CH. A review of pseudotuberculosis at a European zoo: epidemiology and approaches to control. In: Health and conservation of captive and free-ranging wildlife. Proceedings of the American Association of Zoo Veterinarians, American Association of Wildlife Veterinarians, Wildlife Disease Association Joint Conference; 2004 Aug 28-Sep 3; San Diego, California. p. 303-9.

(7.) Childs-Sanford SE, Kollias GV, Abou Madi N, McDonough PL, Garner MM, Mohammed HO. Yersinia pseudotuberculosis in a closed colony of Egyptian fruit bats (Rousettus aegyptiacus). J Zoo Wildl Med. 2009;40:8-14. DOI: 10.1638/20070033.1

(8.) Tennant SM, Grant TH, Robins-Browne RM. Pathogenicity of Yersinia enterocolitica biotype 1A. FEMS Immunol Med Microbiol. 2003;38:127-37. DOI: 10.1016/ S0928-8244(03)00180-9

(9.) Corbel MJ, Brewer RA, Hunter D. Characterisation of Yersinia enterocolitica strains associated with bovine abortion. Vet Rec. 1990;127:526-7.

(10.) Rahuma N, Ghenghesh KS, Ben Aissa R, Elamaari A. Carriage by the housefly (Musca domestica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in Misurata, Libya. Ann Trop Med Parasitol. 2005;99:795 802. DOI: 10.1179/136485905X65134

[1] Current affiliation: Federal Armed Forces Institute of Microbiology, Munich, Germany.

Address for correspondence: Kristin Muhldorfer, Leibniz Institute for Zoo and Wildlife Research, Research Group Wildlife Diseases, AlfredKowalke-Str. 17, D-10315 Berlin, Germany; email:
Table. Strain typing results of Yersinia spp. isolates from
free-ranging bats, Germany *

                                       Y.                    Y.
                               pseudotuberculosis      enterocolitica
Method                             ([dagger])         ([double dagger])

Api 20E profile ([section])     1 0 1 4 1 1 2 1 7     1 1 5 4 7 2 3 1 7
Micronaut E,([paragraph])            100% Y.              99.77% Y.
  % probability                pseudotuberculosis      enterocolitica
16S rRNA gene analysis,
  % similarity to sequences
  in GenBank                   100% (1,058 bp) to     100% (985 bp) to
                              Y. pseudotuberculosis   Y. enterocolitica
                                    YPIII (#)           strain 280 **
Serologic characterization    Serogroup 1, biovar 5     Serotype O:6,
  by agglutination test                                   biovar 1A
Multilocus sequence
  ([dagger])([dagger])            1 2 1 2 5 2 1        Not determined

* Y. pseudotuberculosis obtained from lung, heart, kidney, spleen,
liver, and intestine; Y. enterocolitica obtained from spleen and
intestine. For Y. pseudotuberculosis spleen, liver, and intestine
samples, isolates were obtained in mixed culture accompanied by
colonies of 1-2 nonspecific bacteria. For all remaining tissues, Y.
pseudotuberculosis and Y. enterocolitica were isolated in pure

([dagger]) GenBank accession no. FN561631.

([double dagger]) GenBank accession no. FN561632.

([section]) bioMerieux, Nurtingen, Germany.

([paragraph]) Merlin Diagnostik GmbH, Bornheim-Hersel, Germany.

(#) GenBank accession no. CP000950.

** GenBank accession no. FJ641888.

([dagger])([dagger]) Of gene loci adk, argA, aroA, glnA, thrA, tmk,
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Article Details
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Title Annotation:LETTERS
Author:Muhldorfer, Kristin; Wibbelt, Gudrun; Haensel, Joachim; Riehm, Julia; Speck, Stephanie
Publication:Emerging Infectious Diseases
Article Type:Report
Geographic Code:4EUGE
Date:Mar 1, 2010
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