Printer Friendly

Volumetric magnetic resonance imaging of gastrocnemius muscle in a patient with facioscapulohumeral dystrophy.

Case report

Magnetic resonance imaging (MRI) is a nonradiographic, non-invasive method to visualize and quantify muscle cross-sectional areas and volumes. These areas and volumes can be segmented (i.e. separated) into bone, lean tissue (muscle), and fat tissue according to the intensity (brightness) of the individual voxels [1].

Usually, in patients with muscular dystrophies, manual muscle testing, hand-held dynamometry strength tests, and muscle biopsies of single muscles are used.

MRI can measure specific changes in fat replacement of muscle over time, demonstrating the variability in rates of natural progression between muscle groups and identifying those muscles suitable for use as biomarkers in clinical trials [2, 3].

Moreover, MRI has been used to provide a qualitative assessment of thigh muscles and to examine proton spin lattice relaxation time ([T.sub.1]) in female carriers at a low magnetic field strength [4].

Herein, we present a part of the results of study (NeupoStem). A 15-year-old male with facioscapulohumeral dystrophy (FSHD) has been under the care of the department of Pediatric Rehabilitation for three years. The onset of disease was noted at 12 years of age. His mother also suffers from FSHD and currently uses a wheelchair. The patient had decreased muscle strength of the upper and lower extremities. He was able to walk 300 meters within 6 minute walk test. EMG demonstrated findings consistent with a myopathic disorder. Laboratory tests were also performed. Creatine kinase and muscle creatine kinase were slightly elevated.

Currently, there is no effective treatment available for patients with FSHD [5].

Granulocyte-colony stimulating factor is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream [6]. Recent studies have indicated that granulocyte-colony stimulating factor can potentially be used for the treatment of spinal cord injury, stroke, and neurodegenerative diseases [7-9].

The patient was given subcutaneously granulocyte colony-stimulating factor (5[micro]g/kg body/day) for 5 consecutive days during the first, second, and third months. Laboratory data and adverse events were analyzed. Abdominal ultrasonograph with a spleen assessment was performed before and after seven days of granulocyte colony-stimulating factor administration.

The Ethic Committee of the Medical University of Bialystok approved the study, and written informed consent was obtained from patient and parent.

Therefore, the purpose of this study was to evaluate a gastrocnemius muscle volume of this patient after six months of granulocyte-colony stimulating factor compared to baseline.

MRI

The Siemens Magnetom 0.3T MRI scanner was used to acquire the images of the right calf (Siemens Medical Systems, Inc., Germany) of the subject. The subject was placed in a supine position with a Siemens circularly polarizing (CP) no-tune transmit/receive extremity coil placed over the right calf muscle. The following MR parameters were used to acquire T1 and PD weighted MR images: TSE/TR 4500ms, TE 24 ms, in images SE, TR 468 ms TE 15 ms. The layer thickness was 4 mm, the distance between the layers 1 mm, matrix 512 x 512, 512 x 384.

The analysis of MR images used advanced biomedical imaging software-Analyze 10 Biomedical Imaging Software. (Analyze Direct, Overland Park, KS, USA). Analyze software system is the product of more than 25 years of research, design and development at Mayo Clinic's Biomedical Imaging Resource. It provides direct and automatic (user transparent) support for reading most standard computer image and picture formats, including the emergent DICOM standard for radiologic images. Analyze software system is designed to run on all modern workstation platforms and today's powerful "PC" systems. Used by thousands of imaging researchers worldwide, Analyze represents a mature, feature-rich, tested and proven product with a long history and internationally recognized tradition of continuous innovative development and reliable support [10].

Calf muscle, bones, and subcutaneous fat of the patient were easily seen in MRI findings (Figure 1,ab,c). Gastrocnemius muscle volume increased from 60,567.5 [mm.sup.3] to 70,795.6 [mm.sup.3] after six month of the treatment. The increased of muscle volume of this patient correlated with the improvements of muscle strength and EMG. Our results indicate a good reproducibility for volumetric measures of muscle tissues in the calf using MRI.

Conflict of interest

The authors declared no conflict of interest

REFERENCES

[1.] Commean PK, Tuttle LJ, Hastings MK, Strube MJ, Mueller MJ. Magnetic resonance imaging measurement reproducibility for calf muscle and adipose tissue volume. J Magn Reson Imaging. 2011 Dec; 34(6):1285-94.

[2.] Kan HE, Scheenen TW, Wohlgemuth M, Klomp DW, van Loosbroek-Wagenmans I, Padberg GW, Heerschap A. Quantitative MR imaging of individual muscle involvementin facioscapulo-humeral muscular dystrophy. Neuromuscul Disord. 2009 May; 19(5):357-62.

[3.] Hollingsworth KG, Garrood P, Eagle M, Bushby K, Straub V. Magnetic resonance imaging in duchenne muscular dystrophy: longitudinal assessment of natural history over 18 months. Muscle Nerve. 2013 Oc;t 48(4):586-8.

[4.] Matsumura K, Nakano I, Fukuda N, Ikehira H, Tateno Y, Aoki Y. Duchenne muscular dystrophy carriers. Proton spin-lattice relaxation times of skeletal muscles on magnetic resonance imaging. Neuroradiology. 1989 31(5):373-6.

[5.] Shieh PB. Muscular dystrophies and other genetic myopathies. Neurol Clin. 2013 Nov; 31(4):1009-29.

[6.] Safdar A, Rodriguez G, Zuniga J, Al Akhrass F, Pande A. Use of Healthy-Donor Granulocyte Transfusions to Treat Infections in Neutropenic Patients with Myeloid or Lymphoid Neoplasms: Experience in 74 Patients Treated with 373 Granulocyte Transfusions. Acta Haematol. 2013 Sep; 19; 131 (1):50-8.

[7.] Ringelstein EB, Thijs V, Norrving B, Chamorro A, Aichner F, Grond M, Saver J, Laage R, Schneider A, Rathgeb F, Vogt G, Charisse G, Fiebach JB, Schwab S, Schabitz WR, Kollmar R, Fisher M, Brozman M, Skoloudik D, Gruber F, Serena Leal J, Veltkamp R, Kohrmann M, Berrouschot J; AXIS 2 Investigators. Granulocyte colony-stimulating factor in patients with acute ischemic stroke: results of the AX200 for Ischemic Stroke trial. Stroke. 2013 Oct; 44(10):2681-7.

[8.] Kato K, Yamazaki M, Okawa A, Furuya T, Sakuma T, Takahashi H, Kamiya K, Inada T, Takahashi K, Koda M. Intravenous administration of granulocyte colony-stimulating factor for treating neuropathic pain associated with compression myelopathy: a phase I and IIa clinical trial. Eur Spine J. 2013 Jan; 22(1):197-204.

[9.] Sakuma T, Yamazaki M, Okawa A, Takahashi H, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Mannoji C, Miyashita T, Kadota R, Someya Y, Ikeda O, Yamauchi T, Hashimoto M, Aizawa T, Ono A, Imagama S, Kanemura T, Hanaoka H, Takahashi K, Koda M. Neuroprotective therapy using granulocyte colony-stimulating factor for patients with worsening symptoms of thoracic myelopathy: a multicenter prospective controlled trial. Spine (Phila Pa 1976). 2012 Aug; 1; 37(17):1475-8.

[10.] Erickson BJ, Langer S, Nagy P. The role of open-source software in innovation and standardization in radiology. J Am Coll Radiol. 2005 Nov; 2(11):927-31.

Goscik E. (1) *, Kulak P. (1), Sienkiewicz D. (2), Kulak W. (2), Okurowska-Zawada B. (2)

(1) Department of Pediatric Radiology, Medical University of Bialystok, Poland

(2) Department of Pediatric Rehabilitation, Medical University of Bialystok, Poland

* Corresponding author: Department of Pediatric Radiology Medical University of Bialystok Waszyngtona 17, 15-274 Bialystok, Poland Tel: +48 857450633 e-mail: rtgdsk@wp.pl

Received: 7.11.2013

Accepted: 23.112013
COPYRIGHT 2013 Medical University of Bialystok
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2013 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Author:Goscik, E.; Kulak, P.; Sienkiewicz, D.; Kulak, W.; Okurowska-Zawada, B.
Publication:Progress in Health Sciences
Article Type:Clinical report
Date:Dec 1, 2013
Words:1190
Previous Article:If your name is Beaumont-you must be a geologist! Well, sometimes ...: Funny Stories of the so-called "nominative determinism". Historical joyous...
Next Article:Fine needle aspiration cytology in the diagnosis of Sjogren's syndrome.
Topics:

Terms of use | Privacy policy | Copyright © 2021 Farlex, Inc. | Feedback | For webmasters