Viruses in the etiology of oral carcinogenesis: examination of evidence.
Cancer of the oral cavity is the sixth most common malignancy in developed countries, representing almost 3% of malignant tumours, and more than 95% of them are squamous cell carcinomas. Tobacco and alcohol use are the major risk factors for most cancers of the head and neck including oral cancer. About 15-20% of patients develop oral carcinoma in the absence of exposure to well known risk factors or without any obvious predisposing genetic defect strongly suggests that other risk factors, such as the presence of infectious agents can be involved in oral carcinogenesis. (1) In view of the changes in oncogenes and tumour suppressors in oral carcinogenesis, and the role of carcinogens such as tobacco and alcohol, it is reasonable to question whether viruses play any role. In malignant neoplasia of other squamous epithelia, viruses have been implicated and it is conceivable that viruses might contribute aetiologically in at least some cases of oral carcinoma.
WHAT IS IT THAT IMPARTS A VIRUS ITS ONCOGENIC POTENTIAL?
The first basic requirement for oncogenicity is that the virus be capable of a dynamic interaction with the cell in which the cell not only survives but multiplies and, a persistence of viral influence in that dividing cell.
A tumour virus must be capable of modifying normal mechanisms responsible for control of cell replication.
Since the nucleic acid of all viruses is the source of the code that determines their characteristics, the nucleic acids of tumour viruses should be examined for unique characteristics that might be correlated with oncogenic properties. It has been suggested that the percentage of guanine plus cytosine (G+C) might be significant in determining the oncogenic potential of a given virus. The hypothesis was that G+C content of any oncogenic DNA virus might have to be of approximately the same order as that of mammalian cell DNA, which is 43%.
However, the overall base composition of nucleic acid is only a crude characterization, since it gives no evidence of the spatial arrangement of the bases in the functioning molecule (2)
RELATIONSHIP OF VIRUS TO TRANSFORMED CELL (2)
In case of RNA viruses, the evidence is direct since virus continues to be produced and released. Even with the DNA viruses, all the present evidence points to the persistence of the viral genome in the transformed cell.
The evidence for persistence of viral genetic material in cells transformed by DNA viruses is summarized:
1. Infectious DNA extractable. Rabbit papilloma (Ito)
2. Viral DNA hybridization with tumour cell DNA. Polyoma (Alexrod)
3. Tumor cell RNA hybridization with viral DNA. Polyoma, SV40 (Benjamin)
4. Infectious virus release.SV40 (Gerber)
5. Viral marker rescue. Polyoma (Ting)
6. Viral structural antigen in tumour cell. Adenovirus (Huebner)
7. Virus specific antigens in tumour cells. Polyoma, SV40, Adenovirus (Habel), (Huebner)
CELL FACTORS INVOLVED IN TRANSFORMATION OF A VIRUS
1. Attachment, penetration and uncoating of virus particle.
2. Association of viral genome with cell genome, base sequence homology implicated.
3. Physiologic state of cell, need for cell division, increased DNA target suggested.
4. Karyologic state of cell, chromosome imperfections.
TENETS OF VIRAL CARCINOGENESIS (3)
1. Viruses can cause cancer in animals and humans.
2. Tumour viruses frequently establish persistent infections in natural hosts.
3. Host factors are important determinants of virus-induced tumorigenesis.
4. Viruses are seldom complete carcinogens.
5. Virus infections are more common than virus-related tumour formation.
6. Long latent periods usually elapse between initial virus infection and tumour appearance.
7. Viral strains may differ in oncogenic potential.
8. Viruses may be either direct- or indirect acting carcinogenic agents.
9. Oncogenic viruses modulate growth control pathways in cells.
10. Animal models may reveal mechanisms of viral carcinogenesis.
11. Viral markers are usually present in tumour cells.
12. One virus may be associated with more than one type of tumour.
TUMOUR VIRUSES ARE INFECTIOUS AGENTS
The infectious nature of viruses distinguishes them from all other cancer-causing agents. The recognized tumour viruses tend to establish long-term persistent infections in humans as compared with self-limited infections typical of most common viral diseases. (3)
HUMAN CANCER VIRUSES: HOW DO THEY ACT?
Different mechanisms of transformation of cell are involved in case of direct and indirect acting viruses. Direct acting viruses possess viral oncogenes whereas indirect acting viruses do not possess any oncogene and lack any transforming potential usually. However, no single mechanism is implicated in causation of viral carcinogenesis and many diverse mechanisms are believed to play a role. Even direct acting viruses are not complete carcinogens and are supposed to be initiating or promoting factors which act in synergism with additional changes to transform the normal regulatory pathways in a cell leading to malignancy.
Direct acting viruses such as papilloma virus and polyoma virus encode proteins that act on tumour suppressor proteins such as p53 and Rb which cause their inactivation and lead to cell transformation. The E5 protein of bovine papilloma virus coupled with platelet derived growth factor-[beta] causes the activation of latter and results in a sustained mitogenic signal. (4) However, if this takes place in human epithelial cells is yet to be ascertained. The viral proteins E5, E6 and E7 are involved in malignant transformation in Papilloma virus. EBV elaborates a protein LMP1 which associates with Tumour Necrosis Factor Receptor-Activated Factors (TRAFs) resulting in proliferation. Besides, EBV- encoded nuclear antigens (EBNAs) are important for a cell to be immortalized. Among the EBNAs, EBNA1 has been shown to be oncogenic. (5)
Tumour viruses such as HBV and HTLV-1 act by indirect mechanism of induction of tumour. HBV encodes HBV transactivator protein, the X protein, which is implicated in pathogenesis of hepatocellular carcinoma. This activates the Ras-Raf mitogen activated kinase signalling cascade by either activating the genes related to cellular proliferation or impairing the DNA repair mechanism, due to which, mutations are propagated. (6,7,8) HTLV1 is the only virus known to have a role in human carcinogenesis. Tax, a viral protein, is known to exert deleterious effects on the repair of damaged DNA. (9,10) A more indirect role for HIV in carcinogenesis has been suggested. (11) Immunosuppressed individuals are more prone to developing certain cancers such as EBV-positive lymphomas, HHV-8 and HPV positive tumours. However, much is to be elucidated about the role of retroviruses in human cancers.
PROBLEMS IN ESTABLISHING A VIRAL AETIOLOGY IN HUMAN CARCINOGENESIS
Evans and Mueller (12) and zur Hausen (13) stated the following difficulties that question the role of viruses in causation of cancer.
1. Virus, being ubiquitous in humans, but cancer does not always occur.
2. A long time period between disease onset and appearance of tumour is observed.
3. Time of initial occurrence of tumour may be not known.
4. The host factors which determine an individual being more prone to developing cancer vary.
5. Different virus species differ in their oncogenic potential.
6. Cofactors are needed for virus induced carcinogenesis.
7. Besides viruses, physical and chemical carcinogens may also be involved in causation.
8. Different geographic areas and individuals show different causative factors in causation of same tumour.
9. Detection techniques for viruses such as assays may not provide needed information.
10. The existence of animal model may be doubtful.
Certain criteria were proposed by Sir Austin Bradford Hill (14,15) to confirm disease causation and infectious and/or non-infectious environmental factors in establishment of disease. These were as follows:
1. How often are viruses found in the tumour?
2. Is the association between disease and virus seen repeatedly among different people living in different areas?
3. Is the virus specific to a tumour or not?
4. Does the virus infection occur before the development of tumour?
5. Does the virus load correlate with dose and response relationship?
6. Is it possible biologically that the virus causes the production of tumour?
7. Does the virus and tumour association correlate with the history and biology of disease process?
8. Does experimental data suggest the association of virus with tumour?
When considering a virus as a possible etiologic agent in carcinogenesis, the classical Henle-Koch postulates are not met and even the most recent guidelines pose a problem in arriving at a diagnosis. (16,17) However, data on role of viruses as possible carcinogenic agents has been proposed based on viral biology. Based on this, there is not much doubt regarding viruses targeting cellular pathways by the proteins encoded by them leading to malignant transformation of a cell. Considering the multistep model for viral carcinogenesis a plausible role played by viruses in causation of tumours may be supported. (18) However, more research is needed to elucidate the mechanisms that suggest role of viruses as cancer causing agents.
(1.) Migaldi M, Pecorari M, Forbicini G, Nanni N, Grottola A, Grandi T, Donne GD, Leocata P, Trovato D and Sgambato A. Low prevalence of human papillomavirus infection in the healthy oral mucosa of a Northern Italian population. J Oral Pathol Med 2012; 41:16-20.
(2.) Habel K. The biology of Viral Carcinogenesis. Cancer Research 1968; 28: 1825-1831.
(3.) Butel JS. Viral carcinogenesis: revelation of molecular mechanisms and etiology of human disease. Carcinogenesis 2000; 21 (3): 405-426.
(4.) DiMaio D, Lai CC, Klein O. Virocrine transformation: the intersection between viral transforming proteins and cellular signal transduction pathways. Annu. Rev. Microbiol. 1998;52:397-421.
(5.) Wilson JB, Bell JL, Levine AJ. Expression of Epstein-Barr virus nuclear antigen-1 induces B-cell neoplasia in transgenic mice.EMBO J. 1996; 15:3117-3126.
(6.) Butel JS, Lee TH, Slagle BL. Is the DNA repair system involved in Hepatitis-B-mediated hepatocellular carcinogenesis? Trends Microbiol. 1996; 4:119-124.
(7.) Becker SA, Lee TH, Butel JS, Slagle BL. Hepatitis B virus X protein interferes with cellular DNA repair. J. Virol. 1998; 72:266- 272.
(8.) Jia JL, Wang XW, Harris CC. Hepatitis B virus X protein inhibits nucleotide excision repair. Int. J. Cancer 1999;80: 875-879.
(9.) Kao SY and Marriott SJ. Disruption of nucleotide excision repair by the human T-cell leukemia virus type 1Tax protein. J. Virol. 1999;73: 4299-4304.
(10.) Philpott SM, Buehring GC. Defective DNA repair in cells with human T-cell leukemia / bovine leukemia viruses: role of tax gene. J. Natl. Cancer Inst. 1999:91:933-942.
(11.) Newton R, Beral V, Weiss R. Human immunodeficiency virus infection and cancer. Cancer Surv. 1999; 33:237-262.
(12.) Evans AS, Mueller NE. Viruses and cancer: causal associations. Ann. Epidemiol. 1990,1:71-92.
(13.) Zur Hausen H. Viral oncogenes. In Personnet, J. (ed.) 1999. Microbes and malignancy: Infection as a Cause of Human cancers. Oxford University Press. Oxford, UK, pp 107-130.
(14.) Hill AB, Hill ID. Bradford Hill's Principles of Medical Statistics 1991.12th Ed. Edward Arnold, London, UK.
(15.) Hill AB. Environment and disease: association or causation? Proc. R. Soc. Med. 1965; 58: 295-300.
(16.) Fredericks DN, Relman DA. Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates.Clin. mMicrobiol. Rev. 1996; 9:18-33.
(17.) Brower V. Connecting viruses to cancer: how research moves from association to causation. J. Natl. Cancer Inst. 2004; 96:256-257.
(18.) De Oliveira DE. DNA viruses in human cancer: An integrated overview on fundamental mechanisms of viral carcinogenesis. Cancer Letters 2007; 247:182-196.
doi: 10.5866 2013.541405
Xeeraj Grover , Priyanka Sharma , Harjeet Kaur , Sanjeet Singh ,Shamindra Sengupta , Karan Rajpal 
 Professor & Head
[2&6] Post Graduate Student
 Senior Lecturer
Department of Oral Pathology & Microbiology D.J College of Dental Sciences & Research Modinagar Dr. B.R Ambedkar University, Agra
Received: July 15, 2013
Review Completed: August 12, 2013
Accepted: September 11, 2013
Available Online: February, 2014 (www.nacd.in)
Email for correspondence: firstname.lastname@example.org
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|Author:||Grover, Neeraj; Sharma, Priyanka; Kaur, Harjeet; Singh, Sanjeet; Sengupta, Shamindra; Rajpal, Karan|
|Publication:||Indian Journal of Dental Advancements|
|Date:||Oct 1, 2013|
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