Printer Friendly

Viability and fertility of hybrid New Zealand tree weta Hemideina spp. (Orthoptera: Anostostomatidae).


Natural hybridization between species provides an opportunity to study the mechanisms that maintain independent lineages and may help us understand the process of speciation (Butlin 1987, Barton and Gale 1993). Hybrid animals often have lower fitness than individuals of their parent species (Harrison 1993 and references therein) as a result of incompatible genetic combinations, such as Dobzhansky-Muller incompatibilities (Dobzhansky 1937, Muller 1942, Bolnick and Near 2005), mismatched chromosomes (Shaw and Wilkinson 1980, Searle 1993), incompatible cellular organelles (e.g. mitochondria: Ellison and Burton 2008) and symbiotic bacteria (e.g. intracellular Wolbachia infections: Bordenstein et al. 2001; gut bacteria: Brucker and Bordenstein 2013). However, low hybrid fitness also arises through natural and sexual selection when an intermediate phenotype is a disadvantage (Svedin et al. 2008).

If hybrids are viable they might nevertheless have limited fertility. If fertility of [F.sub.1] hybrids is very low, fertility levels usually improve in subsequent generations of backcross hybrids (Mallet et al. 1998, Mallet 2005, Descimon and Mallet 2009), and even limited fertility provides the possibility for introgression that can have important consequences for species interactions. Adaptive alleles that arise in one population/species can be transferred to another through hybridization, known as adaptive introgression, as seen in wing coloration in Heliconius butterflies (Pardo-Diaz et al. 2012). In some cases, when hybridization reduces fertility a unimodal hybrid (or tension) zone forms. Unimodal hybrid zones are geographically constrained; with most individuals in the zone having mixed ancestry, and the width of the hybrid zone depending on hybrid disadvantage and dispersal of the species (Jiggins and Mallet 2000). If hybridization is more limited, a bimodal hybrid zone may result, where parental forms overlap and predominate with a few individuals of mixed ancestry amongst them. Bimodal hybrid zones are typically associated with assortative mating so, in tree weta, reproductive character displacement is a likely outcome that increases assortative mating (Dieckmann and Doebeli 1999, Jiggins and Mallet 2000). Where two species compete for the same resources, hybridization may prevent one from successfully out-competing the other. Alternatively sexual exclusion, where one species (often the males of that species) out-competes the other for mates, can limit fitness through reproductive interference (Groning and Hochkirch 2008), as observed between the tetrigids: Tetrix ceperoi and Tetrix subulata (Hochkirch et al. 2007).

Tree weta (Orthoptera: Anostostomatidae: Hemideina) are a genus of seven nocturnal arboreal insects, with high morphological and ecological similarity (Field and Bigelow 2001, Dewhurst 2012, Bulgarella et al. 2014). They are hypothesized to have speciated in allopatry during the Pliocene or earlier (Trewick and Morgan-Richards 2005), and they now have broadly parapatric distributions (Trewick and Morgan-Richards 1995, Bulgarella et al. 2014). Hybridisation in the wild between the New Zealand tree weta Hemideina thoracica (White) and both Hemideina crassidens (Blanchard) and Hemideina trewicki Morgan-Richards has recently been confirmed with genetic data, but so far only [F.sub.1] hybrids have been confirmed (Mckean 2014, Mckean et al. 2016). These three Hemideina species each have distinct karyotypes (i.e. different numbers of chromosomes with some differing in size and shape; Morgan-Richards 1995, 1997, 2000, Mckean et al. 2015). Karyotype differences are generally seen as presenting barriers to gene flow by disrupting meiosis and rendering [F.sub.1] hybrids infertile. However, some tree weta species naturally comprise multiple chromosome races that are capable of interbreeding in the wild (Morgan-Richards 1997, 2000, Morgan-Richards et al. 2000, Morgan-Richards and Wallis 2003). The apparent tolerance of chromosome rearrangements displayed in this orthopteran lineage might influence fertility of interspecies hybrids. Karyotype, mtDNA haplotypes, and alleles at four nuclear DNA loci were found to differentiate parent populations of H. thoracica and H. trewicki in a large area of sympatry in Hawke's Bay. These markers (except mtDNA, which is maternally inherited) were heterozygous in individuals who were phenotypically intermediate in abdominal coloration (orange rather than yellow or brown), abdominal bands (faint rather than striking or non-existent), abdominal stripe (a series of spots rather than a stripe or the absence of a stripe) and the number of spines on the prolateral hind tibia (typically between the three spines seen in H. thoracica and the four in H. crassidens/H. trewicki, with a half-sized medial spine on each leg being common, or three spines on one leg and four on the other). A similar situation was seen in the Manawatu area of sympatry between H. thoracica and H. crassidens, where karyotype, mtDNA and three nuclear DNA markers were found to differentiate the two species (with some introgression detected relative to allopatric populations). All individuals, which had an intermediate phenotype (the same phenotype as for H. thoracica x H. trewicki hybrids), were heterozygous for these markers (Mckean et al. 2016). Whether hybridization occurs between H. crassidens and H. trewicki is currently unknown due to the morphological similarities of these two species, and unknown distribution boundaries due in part to clearance of native forest where the two are hypothesized to have historically met (Trewick and Morgan-Richards 1995). A lack of gene flow suggests that H. thoracica x H. trewicki hybrids, which are found at a frequency of 1% of weta in sympatry, are infertile, but genetic and morphological data suggest a low, but potentially significant, level of introgression between H. thoracica and H. crassidens, where hybrid frequency is ~3 in every 100 weta (Mckean et al. 2016).

Introgression is the signal of past hybridization, and an ability to successfully hybridize might be of fundamental importance to the future of a species, while climates and environments continue to change (Grant and Grant 1993, Allendorf et al. 2001, Becker et al. 2013, Taylor et al. 2015, Sivyer et al. 2018). There is evidence that H. crassidens formerly occupied much of central North Island that is now the range of H. thoracica (Bulgarella et al. 2014). Isolated populations of H. crassidens remain in regions of high elevation, which suggests they are adapted to colder environments. With global warming, H. crassidens might continue to be displaced, but this depends on the ecological and sexual interaction between species. Adult tree weta often form harems in tree cavities during the summer and autumn (Wehi et al. 2013), and mixed species harems in areas of overlap suggest that species recognition is not complete (Trewick and Morgan-Richards 1995, Wehi et al. 2017). Of [F.sub.1] hybrids collected in the wild, the majority had an H. thoracica father and H. crassidens mother (Mckean et al. 2016), which suggests sexual exclusion by H. thoracica males; as in Hemideina spp. the females do not appear to actively choose their mates. Although females will sometimes resist mating, resistance times are similar whether mating occurs or not (Field and Jarman 2001). This gives rise to the possibility that hybridization may be an important factor in the coexistence/exclusion of these species.

Haldane (1922) observed that where one sex is absent, rare, or infertile in [F.sub.1] hybrids, it is usually the heterogametic sex. In tree weta this is the male, as tree weta, like most Orthoptera, have an XO sex determination system where females have two copies of the sex chromosome and males one (White 1940, Morgan-Richards 1997, Morgan-Richards and Wallis 2003). Based on this, if there is a difference between the sexes, we would expect male [F.sub.1] hybrids to have lower viability and/or fertility than female [F.sub.1] hybrids.

Given their apparent tolerance of karyotype variation, the high degree of infertility in weta might have another source. Wolbachia is an endosymbiotic intracellular bacteria that infects a large proportion of the arthropod and nematode phyla (Werren et al. 2008). In arthropods, Wolbachia is estimated to infect about 65% of species (Hilgenboecker et al. 2008) including many grasshoppers and crickets (Werren and Windsor 2000, Mandel et al. 2001, Bella et al. 2010). Wolbachia is known to manipulate the reproductive biology of many of its hosts to its own advantage (Werren et al. 2008). Some of the currently known host-reproductive manipulations include male killing, induction of parthenogenesis, feminization of genetic males, forced production of haploid individuals in haplodiploid systems, and cytoplasmic incompatibility (Werren 1997). Wolbachia is hypothesized to have a role in arthropod speciation via induction of cytoplasmic incompatibility (Werren 1998 and references therein). Wolbachia infections appear to be responsible for maintaining hybrid zones between the well-studied grasshopper subspecies Chorthippus parallelus parallelus and Chorthippus parallelus erythropus via two different forms of cytoplasmic incompatibility (Bella et al. 2010). Although recently detected in New Zealand insects (Bridgeman et al. 2018), it is not known whether the tree weta lineage (with many hybrid zones; Morgan-Richards and Wallis 2003) contains this intracellular parasite.

Here, we describe the viability and fertility of hybrids between Hemideina thoracica and H. crassidens, using [F.sub.1] hybrids collected in the wild and held in captivity. We sought evidence of Wolbachia infections to assess whether this common intracellular parasite has potential to limit reproductive compatibility among these weta species.


Sampling and captive conditions.--Eleven [F.sub.1] hybrid tree weta were captured from native forest in Turitea Valley (S40.47184, E175.60943) and Kahutawera Valley (S40.431725, E175.674595), Manawatu, New Zealand (Fig. 1, Table 1). Hybrid identity was tested and confirmed using genetic markers for eight of the 11 individuals which died or were euthanized during the course of this study, and were preserved as specimens in alcohol (Table 1; [F.sub.1] hybrids; Morgan-Richards 1995, Mckean et al. 2016). The other three putative hybrid individuals were assumed to be [F.sub.1] hybrids as their phenotypes were completely consistent with the [F.sub.1] hybrids that had been genetically identified. No cryptic hybrids were identified in previous studies of tree weta from this population (Trewick and Morgan-Richards 1995, Morgan-Richards and Gibbs 2001, Bulgarella et al. 2014, Mckean et al. 2016). Live weta were held in individual containers at a constant temperature of 14[degrees]C. They were given a suitable daytime roost cavity made from harakeke (Phormium tenax) flower stalk, and were fed palatable leaves from at least three native plant species each week and 80% soy protein pellets (Griffin et al. 2011).

Body size of [F.sub.1] hybrids.--No significant difference in body size between adult females of the two parent species has been found in this zone of sympatry (Mckean et al. 2016). To detect signs of impaired growth (hybrid inviability) the hind tibia length of both dead and living adult hybrids was measured with electronic calipers and compared via ANOVA to wild adult females of both parent species measured in a previous study (15 H. thoracica and 19 H. crassidens; Mckean et al. 2016), and to a separate sample of adult males from both species (25 H. thoracica and 22 H. crassidens), that were sampled from the same locations as the hybrids. Hind tibia length is a reliable proxy for body size in tree weta (Minards et al. 2014, Bulgarella et al. 2014.) The sex of hybrids and instar at maturity for male weta were both recorded. Maturity is determined in tree weta by the shape and size of the cerci or ovipositor. Tibia length data for each sex were compared via ANOVA.

Mating behavior.--Six hybrid weta (three males, three females) were provided with one potential mate of each parent species, on different nights, in a Perspex tank (60 cm x 60 cm x 60 cm) (Table 1). Mating trials were observed for 30 min in the evening when tree weta are most active (Kelly 2006a). For male weta, successful transfer of spermatophores was recorded as well as attempts to mate, defined as curling the abdomen to position for mating. Other mating behavior prior to this, such as following the female or rapid twitching of the palps that indicated the male had scented the female, and running the palps over the female's abdomen, were recorded (Field and Jarman 2001 and references therein). As male mating behavior has been well described elsewhere, the male [F.sub.1] hybrids' behavior was compared to what is known from previous work which details the parental species' behavior. Female tree weta do not appear to actively choose or approach male weta (Field and Jarman 2001 and references therein), so their acceptance or active resistance to mating was recorded. Resistance was defined as any behavior that appeared to obstruct mating attempts by the male including moving away, stridulating (a defensive/aggressive gesture in tree weta; Field 2001, Field and Glasgow 2001), and biting and kicking the male to dislodge him. Acceptance was defined as the female staying still and allowing copulation to be initiated and completed, as evidenced by the successful transfer of a spermatophore.

Egg production.--Females of both parent species begin producing eggs as soon as they reach maturity (N.E.M. personal observation, >50 females 2012-2013). Eggs inside the ovarioles of mature females typically vary in developmental stage and range from very small undeveloped yellow eggs through to large black mature eggs with a thick outer casing (Griffin 2011). After laying, the embryo case expands and turns from black to brown and eventually yellow (Stringer 2001). Four [F.sub.1] hybrid adult females and 18 H. crassidens females were given soil slightly deeper than the length of the ovipositor to lay eggs in (Table 2). Conditions were otherwise the same as detailed in captive conditions above. After approximately 100 days (StDev = 35.9) the eggs laid were removed and counted. Each weta was euthanized, dissected and the number of unlaid mature eggs counted under a dissecting microscope. Additional data were obtained from a preserved hybrid female euthanased before she laid eggs (n=5 in Table 2).

Male fertility.--Two adult [F.sub.1] hybrid males, which were adults at the time of the study, were each provided with virgin females of both parent species, as above (Table 3). They were observed until a mating occurred and then left together in the tank overnight. Female weta were removed the next morning and placed in a container with a layer of soil slightly deeper than the length of the ovipositor. After a period of oviposition the female was removed, the eggs counted and placed back into the soil. As little is known about triggers for embryo growth and hatching in weta, the eggs were stored outside, exposed to the ambient winter temperature fluctuations experienced by the wild population from which they were derived. Expansion and hatching were recorded the following summer (approximately 9 months after laying).

Wolbachia detection.--Two methods were used to obtain evidence of infection by the bacteria Wolbachia: amplification of DNA sequences using Wolbachia specific Polymerase Chain Reactions (PCR) primers, and whole genome sequencing and alignment to a reference Wolbachia genome. For amplification of specific Wolbachia DNA sequences, DNA was extracted from three tree weta specimens representing each of the three North Island species (H. thoracica, H. crassidens and H. trewicki). Tissue was taken from the hind femur and testes or ovariole of each tree weta specimen and DNA isolated using a salting out method (Trewick and Morgan-Richards 2005). Wolbachia-specific primers (Appendix 1) were used in PCR with weta DNA, and DNA from an introduced gregarious parasitoid wasp (Nasonia vitripennis) known to be infected with Wolbachia as a positive control. Standard PCR conditions for these primers were followed (Braig et al. 1998, Heddi et al. 1999, Baldo et al. 2006) (Appendix 1). PCRs were repeated to rule out problems with reaction conditions. One PCR product longer than the expected Wolbachia fragment from the CoxA primer pair was amplified. This long DNA fragment was sequenced at the Massey Genome Service with a capillary AB13730 Genetic Analyzer (Applied Biosystems Inc.), and then visualized and trimmed in Geneious 6.1.7 (Biomatters LTD; Kearse et al. 2012) software. The resulting 269 bp sequence was compared to public databases using the Basic Local Alignment Search Tool (BLAST) algorithm on the NCBI website.

Total genomic DNA from two tree weta specimens (an H. thoracica male collected from the Kahutawera Valley and an H. crassidens male collected from a South Island population) were separately processed through parallel, high-throughput sequencing (Illumina HiSeq 2500) for a separate phylogenetic study (Dowle 2013). Briefly, DNA was extracted from a single male individual (testes tissue), fragmented, prepared using the ThruPLEX DNA-seq Kit (Rubicon Genomics) and used to generate 100 bp paired-end sequence on a Hi-Seq 2000 (BGI). This resulted in 5,191,884 100 bp paired-end sequences 200 bp apart for the H. thoracica specimen and 17,434,429 100 bp paired-end sequences for the H. crassidens specimen. An annotated reference Wolbachia genome was obtained from New England Biolabs (, originating from infection of Brugia malayi; Foster et al. 2005). Reads were trimmed to remove index sequences using solexaQA (Cox et al. 2010) before mapping to the Wolbachia genome using the default settings with Bowtie 2 (Langmead and Salzberg 2012). Results were visualised with Tablet v1.7.0_35 (Milne et al. 2010). Sequences that matched parts of the Wolbachia genome were compared with published data using the NCBI (National Library of Medicine) GenBank BLAST search algorithm to determine their similarity to Wolbachia DNA sequences from other hosts. This enabled us to determine whether the sequences came from the Wolbachia genome or another related bacterial species, which could be determined by sequence similarity.


Phenotype [F.sub.1] hybrids.--Hybrids were identified by genetic markers and intermediate phenotypes, and no morphologically cryptic hybrids were identified (Mckean et al. 2016). The sex ratio of [F.sub.1] hybrids in our small sample was even (five females, six males). All but two hybrid weta examined were adults (or reached adulthood in captivity--two weta) providing no evidence of reduced hybrid viability. There was no significant size difference between adult [F.sub.1] hybrid females and adult females of the two parent species from the same location with ANOVA; F = 2.575, P = 0.09 (Fig. 2A), however male [F.sub.1] hybrids were significantly larger than males of either parent species (ANOVA; F = 8.969, P = 0.00049; Fig. 2B). The five adult male hybrids matured at the tenth instar as determined by comparing their hind tibia lengths to data of weta trimorphism in Hemideina crassidens (Kelly and Adams 2010, Bulgarella et al. 2015). Although one male did not reach maturity (Hybrid 10; Table 1), as a ninth instar sub-adult he would have been an adult at the tenth instar, as determined by growth/size charts from previous studies (Spencer 1995, Kelly and Adams 2010).

Mating behavior.--All three [F.sub.1] hybrid males mated with females of both species (Table 1). Each male exhibited normal and similar mating behavior to females of both species he was housed with (Field and Jarman 2001 and references therein), and was accepted by females of both species. In contrast, two of three hybrid females actively resisted mating. The third allowed the H. thoracica male to begin copulation several times, but then dislodged him and proceeded to bite him and display other resistance behaviors. She allowed mating to occur once with the H. crassidens male, and then resisted all subsequent mating attempts, and was the only hybrid female weta that was observed to accept a spermatophore.

Fertility.--None of the five female [F.sub.1] hybrids contained eggs in any stage of development when killed and dissected as adults. This contrasts with 18 H. crassidens females that each laid and/or contained an average of 91 eggs (Table 2). Females that were mated to the hybrid males laid 35-111 eggs (except one H. crassidens female that died soon after mating with Hybrid 2). Some eggs from every female showed signs of expansion after 6-8 months, with many eggs increasing in size and changing color from black to light brown or yellow (Table 3). Four eggs by male Hybrid 2 and his H. thoracica female mate expanded and then hatched to produce offspring. The nymphs were inferred to be phenotypically normal, as no obvious morphological differences were seen under a dissecting microscope. The color of nymphs is uniformly grey (dorsal) and yellowish white (ventral) at this stage regardless of species, so no inferences could be drawn about eventual color phenotype (whether the [F.sub.2] generation look the same as [F.sub.1], or resemble the weta of the parent species). No other eggs hatched during the study, including the eggs produced by the control weta (Table 3).

Wolbachia.--The fbpA and Wol16S primers failed to amplify a DNA fragment when used with tree weta DNA, but produced a DNA fragment with the positive control (a wasp know to be infected with Wolbachia). The Wsp and CoxA primers gave a series of weakly amplified DNA fragments longer than that expected from the Wolbachia genome. A consistent DNA fragment amplified with the CoxA primers was 200 bp longer than the positive control. No close sequence match was found when compared to DNA sequences on the database Genbank, including Wolbachia sequences.

None of the > 17 million H. crassidens next-generation short read DNA sequences mapped to the Wolbachia genome. However, eight 100 bp DNA sequences from genomic H. thoracica DNA shared similarity with Wolbachia. Six identical DNA sequence reads mapped to one location, all with the same ten mismatches. The other two reads mapped to a different location on the Wolbachia genome, differing at nine sites (mismatches). However, the paired-end for all eight of these sequence reads (100-300 bp downstream from the putative-bacteria DNA sequence) did not map to the Wolbachia genome sequence. Comparing the putative Wolbachia sequences to the Genbank database identified these sequences as: 1) 93% similarity with the 16S rRNA gene from various members of the Chlamydiae phylum, with six of these matches belonging to the Rhabdochlamydia genus, and 2) 93% match for three 28S gene fragments from Simkania negevensis, which also belongs to the Chlamydiae phylum. As similarity with Wolbachia sequences was lower (90-91%), it is likely that the H. thoracica weta was infected with a bacteria species from the chlamydia family, not closely related to Wolbachia. Both the 16S and 28S rRNA genes are highly conserved among bacteria, and of the > 22 million DNA short-sequences from the weta none mapped to Wolbachia-specific regions of the Wolbachia genome. A separate study of other Orthoptera confirmed that this level of data was sufficient for detection of Wolbachia infections (Bridgeman et al. 2018).


The size of H. thoracica x H. crassidens hybrids fell within the normal range expected for the parent species (with males at the larger end), and many hybrids were found as adults in the wild, therefore we have no positive evidence of hybrid inviability or abnormal development. There could be some inviability early in development, during the pre-hatching or early instar phases, but it appears that at least by the time [F.sub.1] hybrids have reached the larger instars (5th to 7th), they are as successful as a typical weta of either parent species. Female tree weta all mature at the tenth instar but males can mature at the eighth, ninth or tenth instar (Spencer 1995, Kelly and Adams 2010), resulting in a wide size range of adult males. All [F.sub.1] hybrid males in this study matured at the tenth instar, which may be important in understanding their reproductive success (if any). Male tree weta compete for females via competition for tree cavities (resources) that females use as refuges during the day (Spencer 1995, Field 2001). Tenth instar males have much larger mandibles than eighth instar adult males, which are used during male-male competition, but which also limit their mobility, leading to the hypothesis that smaller males actively search for and mate with females which are away from their tree cavities foraging at night (Field 2001, Kelly 2004, Kelly 2006a, Kelly and Adams 2010). If male [F.sub.1] hybrids all mature at a later instar, it is unclear whether this is an advantage or disadvantage for reproductive success. As the ratios of other male weta maturing at different instars may play a part in determining success in controlling harems, more research into reproductive success among males in this location is needed to determine the outcome of these 10th instar hybrid males.

Our observations of mating were limited to experimental pairs (rather than harems, which are common in the wild; Wehi et al. 2013) and this might have influenced the behaviors exhibited and observed. Female weta were not given a choice of mate, which could have also influenced mating behavior. However, mating behavior appeared to be normal for our limited sample of [F.sub.1] hybrid males when paired with adult H. thoracica and H. crassidens females. One of the hybrid males had been found in the wild with a harem consisting of two adult H. crassidens females. This male produced offspring in captivity, hence it is likely that this male, along with at least some others, are behaving in the wild in a manner typical of males from the parent species. One significant limitation of this study is the lack of control mating crosses and mating behavior comparisons for parental species from the same populations, as previous mating studies were conducted with Hemideina crassidens from southern populations (Field and Jarman 2001, Kelly 2006b, c). There have been few studies of H. thoracica mating behavior (Wehi et al. 2013) and there is a general lack of understanding of mating outcomes in this lineage (Field and Jarman 2001). Unfortunately, the triggers for embryo development and hatching are unknown for tree weta, making laboratory crosses difficult and prone to failure, as evidenced by the control weta embryos failing to hatch. Therefore no inferences can be drawn about the success of these crosses relative to parental crosses, but given that laboratory crosses are sometimes successful when virgin females are mated to single males, it does not appear that sperm competition has to take place in these species to induce fertility in females (Morgan-Richards 2000, Stringer 2001, present study), and can probably be ruled out as an explanation for lack of fertility.

In contrast to the males, the female [F.sub.1] hybrids did not show typical mating behavior, but this may be irrelevant to fertility if they cannot produce eggs. The lack of egg production in all five [F.sub.1] female hybrids is probably biologically important, despite the small sample, because it contrasts with that observed in adult H. crassidens females kept in the same conditions (Table 2). A lack of eggs was also never observed in more than 50 mature parent females of both species that were dissected (N.E.M. personal observation, 2012-2013). The absence of eggs suggests that [F.sub.1] females may typically be infertile, whereas at least some [F.sub.1] male hybrids are fertile, as was evident from the offspring produced in captivity.

Male [F.sub.1] hybrids being partially fertile while females are infertile contrasts with the usual variation between the sexes in reduced fertility (Haldane's rule) and may be of interest for future research. Haldane's rule applies across many animal taxa, including others with a XO sex determination system (Haldane 1922, and one analysis found that it applied in 99% of 223 cases of sex-specific hybrid sterility and 90% of 115 cases of sex-specific hybrid inviability (Laurie 1997). Infertility as opposed to inviability appears to be the most normal sex-skewed outcome, as heterogametic infertility is known to outnumber heterogametic inviability about 10:1 in Drosophila and mammals (Wu and Davis 1993). The mechanism behind Haldane's rule is still unclear although two main hypotheses are X chromosome to autosome imbalance, and incompatibilities between the sex chromosomes. It is also possible that there are multiple causes underlying this phenomenon, but with so few sex determination systems for comparison a conclusive inference is elusive (Coyne 1985, Wu and Davis 1993, Turelli 1998). There is also evidence that the genetic basis of inviability in heterogametic hybrids differs from the genetic basis for infertility (Coyne 1985). Exceptions such as this may eventually shed light on why this rule applies so well to the majority of species. There are some contradictions to Haldane's rule in other XO systems, such as the field crickets Teleogryllus oceanicus and Teleogryllus commodus (Moran et al. 2017), although whether contradictions to Haldane's rule are more common in XO systems is unknown.

One question remaining unanswered in the present study is where the barriers to reproduction are. As bimodal hybrid zones are typically associated with pre-mating rather than post-mating barriers (Jiggins and Mallet 2000 and references therein), the situation here is somewhat unusual. As there does not appear to be assortative mating between these species pairs (Field and Jarman 2001, Morgan-Richards et al. 2001, Wehi et al. 2017), it suggests that barriers are more likely to be the result of genetic constraints. It is not known at what stage the production of [F.sub.1] hybrids is limited, but as intermediate forms are far less common than expected if the species were freely interbreeding (Mckean et al. 2016) some reproductive constraint must operate. Barriers are hypothesised to be at the post-mating pre-zygotic stage or early in development, and it is possible that the weta use unknown behavioral mechanisms to limit interbreeding. A bimodal hybrid zone in two species of chrysomelid beetles (Chyrsochus cobaltinus and C. auratus) also involves stronger post-zygotic barriers than pre-zygotic barriers (Peterson et al. 2005), so the association of assortative mating and bimodal hybrid zones has exceptions. A later study of these same beetles also showed a significant sex-bias in the production of offspring (most had mtDNA haplotypes and hence mothers from one species), despite mating occurring in both directions in the wild, and offspring in both sex-pairings being produced in equal numbers and with equal viability in laboratory crosses at the first instar (Monsen et al. 2007). The proposed explanation was asymmetric post-mating pre-zygotic barriers, or possible asymmetric inviability later in development. As H. thoracica and H. crassidens appear to exhibit both a bimodal hybrid zone in the apparent absence of pre-mating barriers, and also a sex-biased production of [F.sub.1] offspring, there may be some similarities in the mechanisms causing reproductive isolation in these disparate species pairs. Examples such as these may give insights into how bimodal hybrid zones are typically formed and maintained.

Female infertility would prevent mtDNA passing the species boundary (introgressing), and this may explain why no evidence of mtDNA introgression has been seen in previous studies (Bulgarella et al. 2014, Mckean et al. 2016), despite evidence of a low level nuclear DNA and possible phenotypic introgression (Mckean et al. 2016). The low number of [F.sub.1] hybrids seen in the wild suggests that these weta are forming a bimodal hybrid zone (Mckean et al. 2016), and with reduction in fertility of at least a 50% (due to female infertility), production of hybrids is probably costly. The most likely outcome in this scenario would appear to be reproductive character displacement or niche divergence limiting hybridization and loss of reproductive potential, as loss of reproductive compatibility allowing merging of the species would be unlikely with such a significant reduction in fertility (Dieckmann and Doebeli 1999, Jiggins and Mallet 2000). However, if one species uses sexual exclusion to monopolize mates of the other, it could enable for the range expansion of this species. Given that most [F.sub.1] hybrids (8/9) were shown to have a H. thoracica father, this may in part explain how H. thoracica has been able to displace H. crassidens from much of its former range as climate has warmed since the last glacial maximum (Bulgarella et al. 2014). Introgression of adaptive alleles (e.g. cold tolerance) could enable continued expansion of H. thoracica, otherwise the hybrid zone is likely to settle where environmental selection and mate competition are at equilibrium. Further work to determine if male [F.sub.1] hybrids are more likely to successfully reproduce with H. thoracica or H. crassidens females would be valuable, because a bias at this point could influence introgressive asymmetry.

Neither of the two methods employed here provided evidence of Wolbachia infection in Hemideina. The primer pairs that amplify DNA from the common Wolbachia supergroups that infect arthropods (Simoes et al. 2011), and the mapping software used with NGS were sensitive enough to detect infection by another bacterial parasite, so it is highly likely that these weta did not contain Wolbachia although other New Zealand Orthoptera do (Bridgeman et al. 2018). A Chlamydia-like infection was detected in one of the weta in this study however, and as this bacteria also functions as an intracellular parasite (Wyrick 2000), it may be of interest. Weta make good candidates for sexually transmitted diseases, as they generally have some level of promiscuity and have overlapping adult generations (Knell and Webberley 2004).

Our sample of hybrid individuals was small, due to the low frequency of hybrids in the wild (Mckean et al. 2016), however, the results are biologically significant (i.e. [F.sub.1] males having at least some fertility, the infertility of female [F.sub.1] hybrids, thus being an exception to Haldane's Rule), and raise questions about future interaction and survival of these species. In summary, both male and female [F.sub.1] hybrids are capable of reaching maturity, and although Wolbachia is not involved in limiting hybridization, there is at least a 50% (probably higher) reduction in [F.sub.1] hybrid fertility due to female infertility, which might have a strong limiting effect on introgression in the wild. There appears to be a contrast between complete failure by female [F.sub.1] hybrids to produce eggs and partial fertility of some male hybrids, suggesting this system provides another exception to Haldane's rule that in interspecific hybrids the heterogametic sex (in this case males; XO) will have lower fertility than the homogametic sex (female Hemideina XX).

Tree weta are an interesting group for evolutionary studies, in part because they appear to have a high tolerance for chromosome rearrangement that leads to many intraspecific hybrid zones. Much remains unknown about weta biology, particularly with regard to species coexistence and production of hybrids where these weta meet in sympatry lends an extra layer of complexity to the situation. Given that these species meet in different zones of sympatry across the country (and in different species combinations), there is the possibility that different mechanisms have, or will, evolve in different areas, which could be another promising area for further study.


We thank Mariana Bulgarella, Emily Koot, Shaun Neilson, Anne Kim and Priscilla Wehi for help collecting hybrid weta. This work was supported by the Massey University Research Fund (M. Morgan-Richards; MURF2013: Can weta coexist?). The paper was much improved by constructive comments from Mike Ritchie and two anonymous reviewers.


Allendorf FW, Leary RF, Spruell P, Wenburg JK (2001) The problems with hybrids: setting conservation guidelines. Trends in Ecology and Evolution 16: 613-622.

Baldo L, Hotopp JCD, Jolley KA, Bordenstein SR, Biber SA, Choudhury RR, Hayashi C, Maiden MC, Tettelin H, Werren JH (2006) Multilocus sequence typing system for the endosymbiont Wolbachia pipientis. Applied and Environmental Microbiology 72: 7098-7110.

Barton NH, Gale KS (1993) Genetic analysis of hybrid zones. In: Harrison RG (Ed.) Hybrid zones and the evolutionary process. Oxford University Press, New York, 13-45.

Becker M, Gruenheit N, Steel M, Voelckel C, Deusch O, Heenan PB, McLenachan PA, Kardailsky O, Leigh JW, Lockhart PJ (2013) Hybridization may facilitate in situ survival of endemic species through periods of climate change. Nature Climate Change 3: 1039-43.

Bella JL, Martinez-Rodriguez P, Arroyo-Yebras F, Bernal A, Sarasa J, Fernandez-Calvin B, Mason PL, Zabal-Aguirre M (2010) Wolbachia infection in the Chorthippus parallelus hybrid zone: evidence for its role as a reproductive barrier. Journal of Orthoptera Research 19: 205-212.

Bolnick DI, Near TJ (2005) Tempo of hybrid inviability in centrarchid fishes (Teleostei: Centrarchidae). Evolution 59: 1754-1767.

Bordenstein SR, O'Hara FP, Werren JH (2001) Wolbachia-induced incompatibility precedes other hybrid incompatibilities in Nasonia. Nature 409: 707-710.

Braig HR, Zhou W, Dobson SL, O'Neill SL (1998) Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia pipientis. Journal of Bacteriology 180: 2373-2378.

Bridgeman B, Morgan-Richards M, Wheeler D, Trewick SA (2018) First detection of Wolbachia in the New Zealand biota. PLoS ONE 13: e0195517. pone.0195517

Brucker RM, Bordenstein SR (2013) The hologenomic basis of speciation: gut bacteria cause hybrid lethality in the genus Nasonia. Science 341: 667-669.

Bulgarella M, Trewick SA, Godfrey AJR, Sinclair BJ, Morgan-Richards M (2015) Elevational variation in adult body size and growth rate but not in metabolic rate in the tree weta Hemideina crassidens. Journal of Insect Physiology 75: 30-38.

Bulgarella M, Trewick SA, Minards NA, Jacobson MJ, Morgan-Richards M (2014) Shifting ranges of two tree weta species (Hemideina spp.): competitive exclusion and changing climate. Journal of Biogeography 41: 524-535.

Butlin R (1987) Speciation by reinforcement. Trends in Evolution and Ecology 2: 8-13.

Cox MP, Peterson DA, Biggs PJ (2010) SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data. BMC Bioinformatics 11: 485.

Coyne JA (1985) The genetic basis of Haldane's rule. Nature 314: 736.

Descimon H, Mallet J (2009) Bad species. In: Shreeve TG (Ed.) Ecology and Evolution of European Butterflies. Oxford University Press, London, 219-249.

Dewhurst R (2012) The diet of tree weta: natural and captive folivory preferences of Hemideina crassidens and Hemideina thoracica. MSc Thesis, Massey University, Palmerston North.

Dieckmann U, Doebeli M (1999) On the origin of species by sympatric speciation. Nature 400: 354-357.

Dobzhansky T (1937) Genetic nature of species differences. American Naturalist 71: 404-420.

Dowle EJ (2013) Rates of Molecular Evolution and Gene Flow. Doctoral Dissertation, Massey University, Palmerston North.

Ellison CK, Burton RS (2008) Interpopulation hybrid breakdown maps to the mitochondrial genome. Evolution 62: 631-638.

Field LH (2001) Aggression behaviour in New Zealand tree wetas. In: Field LH (Ed.) Biology of Wetas, King Crickets and their Allies. CAB International, 271-295.

Field LH, Bigelow RS (2001) Morphometric analysis of Hemideina spp. in New Zealand. In: Field LH (Ed.) Biology of Wetas, King Crickets and their Allies. CAB International, 63-177.

Field LH, Glasgow S (2001) Defence behaviour. In: Field LH (Ed.) Biology of Wetas, King Crickets and their Allies. CAB International, 271-295.

Field LH, Jarman TH (2001) Mating behaviour. In: Field LH (Ed.) Biology of Wetas, King Crickets and their Allies. CAB International, 271-295.

Foster J, Ganatra M, Kamal I, Ware J, Makarova K, Ivanova N, Bhattacharyya A, Kapatral V, Kumar S, Posfai J, Vincze T (2005) The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode. PLoS Biology 3: e121.

Grant BR, Grant PR (1993) Evolution of Darwin's finches caused by a rare climatic event. Proceedings of the Royal Society of London B: Biological Sciences 251: 111-117.

Griffin MJ (2011) Wellington Tree Weta (Hemideina crassidens) diet and the effect of some of their dietary choices. MSc Thesis, Massey University, Palmerston North.

Groning J, Hochkirch A (2008) Reproductive interference between animal species. The Quarterly Review of Biology 83: 257-282.

Haldane JB (1922) Sex ratio and unisexual sterility in hybrid animals. Journal of Genetics 12: 101-109.

Harrison RG (Ed.) (1993) Hybrid Zones and the Evolutionary Process. Oxford University Press, New York.

Heddi A, Grenier AM, Khatchadourian C, Charles H, Nardon P (1999) Four intracellular genomes direct weevil biology: nuclear, mitochondrial, principal endosymbiont, and Wolbachia. Proceedings of the National Academy of Sciences 96: 6814-6819.

Hilgenboecker K, Hammerstein P, Schlattmann P, Telschow A, Werren JH (2008) How many species are infected with Wolbachia? - A statistical analysis of current data. FEMS Microbiology Letters 281: 215-220.

Hochkirch A, Groning J, Bucker A (2007) Sympatry with the devil: reproductive interference could hamper species coexistence. Journal of Animal Ecology 76: 633-642.

Jiggins CD, Mallet J (2000) Bimodal hybrid zones and speciation. Trends in Ecology and Evolution 15: 250-255.

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647-1649.

Kelly CD (2004) Allometry and sexual selection of male weaponry in Wellington tree weta, Hemideina crassidens. Behavioral Ecology 16: 145-152.

Kelly CD (2006a) Movement patterns and gallery use by the sexually dimorphic Wellington tree weta. New Zealand Journal of Ecology 30: 273-278.

Kelly CD (2006b) Fighting for harems: assessment strategies during male-male contests in the sexually dimorphic Wellington tree weta. Animal Behaviour 72: 727-736.

Kelly CD (2006c) Resource quality or harem size: what influences male tenure at refuge sites in tree weta (Orthoptera: Anostostomatidae)? Behavioral Ecology and Sociobiology 60: 175e183.

Kelly CD, Adams DC (2010) Sexual selection, ontogenetic acceleration, and hypermorphosis generates male trimorphism in Wellington tree weta. Evolutionary Biology 37: 200-209.

Knell RJ, Webberley KM (2004) Sexually transmitted diseases of insects: distribution, evolution, ecology and host behaviour. Biological Reviews 79: 557-581.

Langmead B, Salzberg S (2012) Fast gapped-read alignment with Bowtie 2. Nature Methods 9: 357-359.

Laurie CC (1997) The weaker sex is heterogametic: 75 years of Haldane's rule. Genetics 147: 937-951.

Mallet J (2005) Hybridization as an invasion of the genome. Trends in Ecology and Evolution 20: 229-337.

Mallet J, McMillan WO, Jiggins CD (1998) Mimicry and warning color at the boundary between races and species. In: Howard D, Berlochers S (Eds) Endless Forms: Species and Speciation. Oxford University Press, Oxford, 390-403.

Mandel MJ, Ross CL, Harrison RG (2001) Do Wolbachia infections play a role in unidirectional incompatibilities in a field cricket hybrid zone? Molecular Ecology 10: 703-709.

Mckean NE (2014) Hybridization of North Island Tree Weta. MSc Thesis, Massey University, Palmerston North.

Mckean NE, Trewick SA, Morgan-Richards M (2015) Comparative cytogenetics of North Island tree weta in sympatry. NZ Journal of Zoology 42: 73-84.

Mckean NE, Trewick SA, Morgan-Richards M (2016) Little or no gene flow despite F1 hybrids at two interspecific contact zones. Ecology and Evolution 6: 2390-2404.

Milne I, Bayer M, Cardle L, Shaw P, Stephen G, Wright F, Marshall D (2010) Tablet--next generation sequence assembly visualization. Bioinformatics 26: 401-402.

Minards NA, Trewick SA, Godfrey AJR, Morgan-Richards M (2014) Convergent local adaptation in size and growth rate but not metabolic rate in a pair of parapatric Orthoptera species. Biological Journal of the Linnaean Society 113: 123-135.

Monsen KJ, Honchak BM, Locke SE, Peterson MA (2007) Cytonuclear disequilibrium in Chrysochus hybrids is not due to patterns of mate choice. Journal of Heredity 98: 325-330.

Moran PA, Ritchie MG, Bailey NW (2017) A rare exception to Haldane's rule: Are X chromosomes key to hybrid incompatibilities? Heredity 118: 554.

Morgan-Richards M (1995) A new species of tree weta from the North Island of New Zealand (Hemideina Stenopelmatidae: Orthoptera). New Zealand Entomologist 18: 15-23.

Morgan-Richards M (1997) Intraspecific karyotype variation is not concordant with allozyme variation in the Auckland tree weta of New Zealand, Hemideina thoracica (Orthoptera: Stenopelmatidae). Biological Journal of the Linnean Society 60: 423-442.

Morgan-Richards M (2000) Robertsonian translocations and B chromosomes in the Wellington tree weta, Hemideina crassidens (Orthoptera: Anostostomatidae). Hereditas 132: 49-54.

Morgan-Richards M, Gibbs GW (2001) A phylogenetic analysis of New Zealand giant and tree weta (Orthoptera: Anostostomatidae: Deinacrida and Hemideina) using morphological and genetic characters. Invertebrate Systematics 15: 1-12.

Morgan-Richards M, King T, Trewick SA (2001) The evolutionary history of tree weta: A genetic approach. In: Field LH (Ed.) Biology of Wetas, King Crickets and their Allies. CAB International, 111-126.

Morgan-Richards M, Trewick SA, Wallis GP (2000) Characterization of a hybrid zone between two chromosomal races of the weta Hemideina thoracica following a geologically recent volcanic eruption. Heredity 85: 586-592.

Morgan-Richards M, Wallis GP (2003) Degree of cytogenetic differentiation fails to predict hybrid zone width in the weta Hemideina thoracica (Orthoptera: Anostostomatidae). Evolution 57: 849-861.

Muller HJ (1942) Isolating mechanisms, evolution, and temperature. Biological Symposia 6: 71-125.

Pardo-Diaz C, Salazar C, Baxter SW, Merot C, Figueiredo-Ready W, Joron MW, McMillan O, Jiggins CD (2012) Adaptive introgression across species boundaries in Heliconius butterflies. PLoS Genetics 8: e1002752.

Peterson MA, Honchak BM, Locke SE, Beeman TE, Mendoza J, Green J, Monsen KJ (2005) Relative abundance and the species-specific reinforcement of male mating preference in the Chrysochus (Coleoptera: Chrysomelidae) hybrid zone. Evolution 59: 2639-2655.

Searle JB (1993) Chromosomal hybrid zones in eutherian mammals. In: Harrison RG (Ed.) Hybrid Zones and the Evolutionary Process. Oxford University Press, New York, 309-353.

Shaw DD, Wilkinson P (1980) Chromosome differentiation, hybrid breakdown and the maintenance of a narrow hybrid zone in Caledia. Chromosoma 80: 1-31.

Simoes PM, Mialdea G, Reiss D, Sagot MF, Charlat S (2011) Wolbachia detection: an assessment of standard PCR Protocols. Molecular Ecology Resources 11: 567-572.

Sivyer L, Morgan-Richards M, Koot E, Trewick SA (2018) Anthropogenic cause of range shifts and gene flow between two grasshopper species revealed by environmental modelling, geometric morphometrics and population genetics. Insect Conservation & Diversity

Spencer A (1995) Sexual Maturity in the Male Tree Weta Hemideina crassidens (Orthoptera: Stenopelmatidae). MSc Thesis, Victoria University of Wellington, Wellington.

Stringer AN (2001) The reproductive biology and the eggs of New Zealand Anostostomatidae. In: Field LH (Ed.) Biology of Wetas, King Crickets and their Allies. CAB International, 379-397.

Svedin N, Wiley C, Veen T, Gustafsson L, Qvarnstrom A (2008) Natural and sexual selection against hybrid flycatchers. Proceedings of the Royal Society of London B: Biological Sciences 275: 735-744.

Taylor SA, Larson EL, Harrison R G (2015) Hybrid zones: windows on climate change. Trends in Ecology and Evolution 30: 398-406.

Trewick SA, Morgan-Richards M (1995) On the distribution of tree weta in the North Island, New Zealand. Journal of the Royal Society of New Zealand 25: 485-493.

Trewick SA, Morgan-Richards M (2005) After the deluge: mitochondrial DNA indicates Miocene radiation and Pliocene adaptation of tree and giant weta (Orthoptera: Anostostomatidae). Journal of Biogeography 32: 295-309.

Turelli M (1998) The causes of Haldane's rule. Science 282: 889-891.

Wehi PM, Jorgensen M, Morgan-Richards M (2013) Sex- and season-dependent behavior of a flightless insect, the Auckland tree weta. New Zealand Journal Ecology 37: 75-83.

Wehi PM, Monks A, Morgan-Richards M (2017) Male tree weta are attracted to cuticular scent cues but do not discriminate according to sex or among two closely related species. Ethology 123: 825-834.

Werren JH (1997) Biology of Wolbachia. Annual Review of Entomology 42: 587-609.

Werren JH (1998) Wolbachia and speciation. In: Howard D, Berlocher S (Eds) Endless Forms: Species and Speciation. Oxford University Press, 45-260.

Werren JH, Baldo L, Clark ME (2008) Wolbachia: master manipulators of invertebrate biology. Nature Reviews Microbiology 6: 741-751.

Werren JH, Windsor DM (2000) Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proceedings of the Royal Society of London B: Biological Sciences 267: 1277-1285.

White MJD (1940) The origin and evolution of multiple sex-chromosome mechanisms. Journal of Genetics 40: 303.

Wu CI, Davis AW (1993) Evolution of postmating reproductive isolation: the composite nature of Haldane's rule and its genetic bases. The American Naturalist 142: 187-212.

Wyrick PB (2000) Intracellular survival by Chlamydia. Cellular Microbiology 2: 275-282.

Appendix 1


(1) Ecology Group, Institute of Agriculture and Environment, Massey University, Palmerston North 4442, New Zealand.

Corresponding author: Natasha E. Mckean (

Academic editor: Corinna S. Bazelet | Received 10 June 2017 | Accepted 21 January 2018 | Published 29 June 2018
Table 1. Sampling information, size and results for mating behavior in
both sexes and egg production in hybrid females.

  Weta      Sample        Location       Genetically  Sex     Age

Hybrid 1     Live     Kahutawera valley      Yes       M     Adult
Hybrid 2     Live      Turitea valley        Yes       M     Adult
Hybrid 3     Live      Turitea valley        Yes       M     Adult
Hybrid 4     Live     Kahutawera valley      Yes       F     Adult
Hybrid 5     Live     Kahutawera valley      No        F     Adult
Hybrid 6     Live     Kahutawera valley      No        F     Adult
Hybrid 7   Preserved  Kahutawera valley      Yes       F     Adult
Hybrid 8   Preserved  Kahutawera valley      No        F     Adult
Hybrid 9   Preserved  Kahutawera valley      Yes       M   Juvenile
Hybrid 10    Live     Kahutawera valley      Yes       M   Sub-adult
Hybrid 11  Preserved  Kahutawera valley      Yes       M     Adult

  Weta     Tibia    Instar          Behavior         Age since  Eggs
           Length     at                             Maturity
            (mm)   Maturity                           (Final

Hybrid 1   23.63      10       Normal; mated (*)        NA       NA
Hybrid 2   24.01      10       Normal; mated (*)        NA       NA
Hybrid 3   23.62      10       Normal; mated (*)        NA       NA
Hybrid 4   22.92      10      Resisted Mating (+)    6 months    No
Hybrid 5   21.26      10      Resisted Mating (+)    4 months    No
Hybrid 6   23.76      10     Partial Resistance (+)  3 months    No
Hybrid 7   21.26      10               NA            6 months    No
Hybrid 8   22.29      10               NA            3 months    No
Hybrid 9   16         10               NA               NA       NA
Hybrid 10  18.42      10               NA               NA       NA
Hybrid 11  21.11      10               NA               NA       NA

Legend: (*) See Table 3, (+) See section 'Mating Behavior' in methods.

Table 2. Average number of eggs +/- standard deviation for H. crassidens
females vs. [F.sub.1] hybrid females.

            Sample    Age since         Eggs        Eggs       Eggs
             size   maturity (days)   (unlaid)     (laid)     (total)

H.            18    201 +/- 70.7     26 +/- 30.9  65 +/- 32  91 +/- 26.5
[F.sub.1]      5    139 +/- 47.8      0            0          0

Table 3. Results of captive breeding experiments with [F.sub.1] hybrid
H. thoracica x H. crassidens fathers and mothers of both parent species.
Growth of eggs was both physical expansion and changing color from black
to brown or yellow.

  Male        Female        No.  Growth  Hatched

            H. crassidens   50    Yes       0
Hybrid 1 x  H. crassidens   35    Yes       0
            H. thoracica   111    Yes       0
Hybrid 2 x  H. thoracica    37    Yes       4
            H. crassidens    -     -        -

Table 4. Information for weta DNA amplification with Wolbachia-specific
PCR primers.

             Locus                     Source         Forward / Reverse

Wolbachia surface protein (wsp)    Braig et al. 1998  Wsp81F / Wsp691R
Fructose-bisphosphate aldolase     Baldo et al. 2006   fbpAF1 / fbpAR1
Cytochrome c oxidase, subunit I    Baldo et al. 2006   CoxAF1 / CoxAR1
Wolbachia specific portion of 16S  Heddi et al. 1999  Wol16SF / Wol16SR
ribosomal RNA gene (wol16S)
COPYRIGHT 2018 The Orthopterists' Society
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2018 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Research Article
Author:Natasha E. Mckean, Steven A. Trewick, Melissa J. Griffin, Eddy J. Dowle, Mary Morgan-Richards
Publication:Journal of Orthoptera Research
Date:Jul 1, 2018
Previous Article:Revision of the tropical African genus Tetraconcha (Orthoptera: Tettigoniidae: Phaneropterinae) with the description of ten new species.
Next Article:Revision of Aresceutica (Orthoptera: Acrididae: Catantopinae) with comments on related genera.

Terms of use | Privacy policy | Copyright © 2020 Farlex, Inc. | Feedback | For webmasters