Universita Degli Studi Di Milano obtains United States patent.
Universita Degli Studi Di Milano, Bicocca (Milan, Italy) has
patented a method of generating ascorbic acid from yeast. In one
embodiment, the yeast is a Zygosaccharomyces spp. or a Kluyveromyces
spp. growing in a medium comprising an ascorbic acid precursor. In a
second embodiment the yeast is a recombinant yeast growing in a medium
comprising an ascorbic acid precursor. Preferably the recombinant yeast
is transformed with a coding region encoding an enzyme selected from
L-galactose dehydrogenase, L-galactono-1,4-lactone dehydrogenase,
D-arabinose dehydrogenase, D-arabinono-1,4-lactone oxidase or
L-gulono-1,4-lactone oxidase. The ascorbic acid precursor is preferably
D-glucose, L-galactose, L-galactono-1,4-lactone, or
L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid
is accumulated in the medium at levels greater than background.
Preferably, the yield of the conversion of the precursor to ascorbic
acid is preferably at least about 35%. (US 7,579,171)