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Unique strain of Crimean-Congo hemorrhagic fever Virus, Mali.

To the Editor: Crimean-Congo hemorrhagic fever (CCHF) is an acute viral infection that causes mild to severe hemorrhagic fever characterized by petechiae, ecchymosis, disseminated intravascular coagulation, and multi-organ failure (1). The etiologic agent, CCHF virus (CCHFV; family Bunyaviridae, genus Nairovirus), is maintained in enzootic cycles involving agricultural and wild animals and the vector, Hyalomma ticks. (2). CCHF predominantly affects persons who have 1) substantial contact with ticks and/or agricultural animals in areas where CCHF is endemic or 2) close contact with infected persons, predominantly close relatives and health care workers. The case-fatality rate for CCHF is generally accepted as 30% (1).

CCHF has a wide geographic distribution; cases have been reported in >30 countries across Africa, southeastern Europe, the Middle East, and western Asia. In the western African countries of Nigeria, Mauritania, and Senegal, serologic evidence of CCHFV infections in humans and agricultural animals has been documented frequently (3-5); however, reports of the disease in humans have been limited to Senegal and Mauritania (6,7). In neighboring Mali, where the tick vector is known to be present, little information exists regarding the presence of CCHFV. Thus, to determine if the virus is circulating undetected in Mali, we conducted a study to determine if CCHFV is present in Hyalomma ticks in the country.

In November 2011 and March 2012, unfed Hyalomma ticks (adults and nymphs) were collected from 20 cattle at the Daral livestock market (12[degrees] 49.855' N, 08[degrees] 05.651' W) near the town of Kati, Mali, [approximately equal to]25 km from the capital, Bamako. In the field, ticks were visually identified to genus and pooled accordingly (3-4 ticks per pool, all collected from the same animal). A total of 23 tick pools, representing 80 ticks, were manually homogenized, and RNA was extracted and tested for the presence of CCHFV RNA by using in-house assays that selected for 3 virus genes. Of the 23 tick pools tested, 1 was positive for CCHFV by all 3 assays. Phylogenetic analysis of the complete nucleocapsid protein gene (KF793333) showed that the CCHFV strain from Mali most closely resembled a strain from Mauritania (GenBank accession no. ArD39554), sharing 98% sequence identity (Figure, panel A).

Further analysis of fragments of the medium segment (pre-Gn coding region, KF793334) and large segment (polymerase coding region, KF793335) confirmed these findings, showing sequence identities of 91% and 98%, respectively, with ArD39554 (Figure, panels B, C). In a Biosafety Level 4 facility at Rocky Mountain Laboratories, Hamilton, Montana, USA, the original homogenates from the positive pool were passaged in multiple cell lines. After 3 passages, no discernible cytopathic effect was observed and, aside from the initial passage, CCHFV RNA was not detected.

Genetic identification of ticks in the CCHFV RNA-positive pool was conducted as described (8,9). Amplified sequences most closely resembled those of H. dromedarii, (97.2%-100% sequence identity), although genetically, we cannot exclude the possibility that H. truncatum and H. rufipes were present with individual sequence identities of >97% to published sequences.

The Daral cattle market in Kati is the largest of its kind in Mali, and animals from across the country come into the market every week. Although the market provided a convenient opportunity for collecting ticks, we cannot determine where the infected ticks, and possibly cattle, contracted CCHFV because the animals traversed great distances on foot before arriving at the market. Nevertheless, this study demonstrates the presence of a distinct strain of CCHFV in Hyalomma ticks in Mali, thereby expanding the geographic distribution of this virus in western Africa. Not surprisingly, the highest sequence identity for the CCHFV strain from Mali is to strains known to circulate in neighboring countries (10). We propose Daral 2012 Mali as the temporary designation for this sequence. Unfortunately, our attempts to isolate the virus were unsuccessful, most likely because of processing and storage conditions for homogenates used in these studies.

Species of Hyalomma ticks are widely distributed across western Africa, and although reports of CCHF are limited to a few countries, CCHFV is most likely circulating undetected in vast areas of this region. No cases of CCHF have been reported in Mali; however, on the basis of our findings, the potential for human infections exists. Thus, CCHF should be considered in the differential diagnosis of febrile illnesses, with or without hemorrhagic symptoms, in residents of Mali and for persons with a recent history of travel to this country.

The ease of CCHFV transmission and the high case-fatality rate associated with infection could have a potentially substantial effect on public health. Future studies in Mali are required to define the geographic distribution of infected ticks and animals and to isolate CCHFV to help focus public health preparedness and countermeasures. In addition, across Mali, operational protocols should be reviewed for persons working at jobs in which the risk for CCHFV transmission is high (e.g., occupations with direct contact with agricultural animals and/or animal blood products), and appropriate countermeasures should be put in place to prevent transmission among such persons.


We are indebted to the chief of Daral for allowing us access to the Kati cattle market and to the individual ranchers for providing ticks from their cattle. In addition, we thank Seydou Doumbia, Sekou Traore, Richard Sakai, Joseph Shott, and Mark Pineda for logistics support; Robert J. Fischer, Brandi Williamson, Eric Dahlstrom, and Stephen Porcella for technical assistance; and Heather Murphy for help preparing the figures.

This work was funded by the International Centers for Excellence in Research program, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health.

Marko Zivcec, Ousmane Malga, Ashley Kelly, Friederike Feldmann, Nafomon Sogoba, Tom G. Schwan, Heinz Feldmann, and David Safronetz

Author affiliations: National Institutes of Health, Hamilton, Montana, USA (M. Zivcec, A. Kelly, F. Feldmann, T.G. Schwan, H. Feldmann, D. Safronetz); University of Manitoba, Winnipeg, Manitoba, Canada (M. Zivcec, H. Feldmann); and University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali (O. Maiga, N. Sogoba)



(1.) Ergonul O. Crimean-Congo hemorrhagic fever virus: new outbreaks, new discoveries. Curr Opin Virol. 2012;2:215-20. http://

(2.) Whitehouse CA. Crimean-Congo hemorrhagic fever. Antiviral Res. 2004;64:145-60. 2004.08.001

(3.) David-West TS, Cooke AR, David-West AS. Seroepidemiology of Congo virus (related to the virus of Crimean haemorrhagic fever) in Nigeria. Bull World Health Organ. 1974;51:543-6.

(4.) Gonzalez JP, LeGuenno B, Guillaud M, Wilson ML. A fatal case of Crimean-Congo haemorrhagic fever in Mauritania: virological and serological evidence suggesting epidemic transmission. Trans R Soc Trop Med Hyg. 1990;84:573-6. http://

(5.) Wilson ML, LeGuenno B, Guillaud M, Desoutter D, Gonzalez JP, Camicas JL. Distribution of Crimean-Congo hemorrhagic fever viral antibody in Senegal: environmental and vectorial correlates. Am J Trop Med Hyg. 1990;43:557-66.

(6.) Nabeth P, Cheikh DO, Lo B, Faye O, Vall IO, Niang M, et al. Crimean-Congo hemorrhagic fever, Mauritania. Emerg Infect Dis. 2004;10:2143-9. http://dx.doi. org/10.3201/eid1012.040535

(7.) Tall A, Sall AA, Faye O, Diatta B, Sylla R, Faye J, et al. Two cases of Crimean-Congo haemorrhagic fever (CCHF) in two tourists in Senegal in 2004. Bull Soc Pathol Exot. 2009;102:159-61.

(8.) Rees DJ, Dioli M, Kirkendall LR. Molecules and morphology: evidence for cryptic hybridization in African Hyalomma (Acari: Ixodidae). Mol Phylogenet Evol. 2003;27:131-42. http://dx.doi. org/10.1016/S1055-7903(02)00374-3

(9.) Barker SC. Distinguishing species and populations of rhipicephaline ticks with its 2 ribosomal RNA. J Parasitol. 1998;84:887-92. 3284614

(10.) Deyde VM, Khristova ML, Rollin PE, Ksiazek TG, Nichol ST. Crimean-Congo hemorrhagic fever virus genomics and global diversity. J Virol. 2006;80:8834-42. http://

Address for correspondence: David Safronetz, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S 4th St, Hamilton, MT 59840, USA; email: safronetzd@


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Title Annotation:LETTERS
Author:Zivcec, Marko; Malga, Ousmane; Kelly, Ashley; Feldmann, Friederike; Sogoba, Nafomon; Schwan, Tom G.;
Publication:Emerging Infectious Diseases
Article Type:Letter to the editor
Geographic Code:6MALI
Date:May 1, 2014
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