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Underground microclimate influence of Romanian Ocna Dej and Cacica salt mines on wistar white rats induced pathologies experimental model.

Keywords: Salt Mine Speleotherapy; Induced Pathology; Air Composition; Electrolyte Balance; Protein Electrophoresis

Introduction and Objectives

The aim of this study is to determine the effect of underground microclimate from romanian Cacica and Ocna Dej salt mines on protein metabolism and hidroelectrolyte balance in different age Wistar white rats with induced pathology subjected to speleotherapy cure in mentioned salt mines.

Materials and Methods

The study was performed on 80 young and adult Wistar white rats sensibilized with induced allergic pathology by ovalbumine or skin pathology (wounds, burns) divided in experimental and control groups. The rats from experimental groups were subjected to a speleotherapy cure in Ocna Dej or Cacica salt mines.

The air composition of underground environment in the Cacica and Ocna Dej salt mines were determined with RAE gas detection tubes and the following gases and volatile compounds were assayed: O2 (%), CO2 (%), CO (ppmv), SO2 (ppmv), O3 (ppmv), Cl2 (ppmv), NH3 (ppmv), NOX (ppmv), HC (ppmv), H2O (mg/L). Total protein concentration was assayed by microplate biuret reaction, the readings being made with a Modulus Multimode Microplate Reader (Turner Biosystems). Serum protein electrophoresis was performed on cellulose acetate using a Genio S (Interlab) electrophoresis automated system.

In order to study the electrolyte balance animals were kept for 24 hours in individual metabolic cages without food and with free access to a saline solution. After 24 hours were measured water volume (ml/24 h) and the amount of sodium intake (mEq/24 h), urine volume (ml/24 h) and concentrations of sodium and potassium in urine (mEq/24 h) using a Ciba Corning 480 flame photometer. From these values were calculated the urinary Na/K ratio as an expression of the mineralocorticoid response of adrenals in the experimental conditions.


The experimental data obtained show that the air composition of the both studied salines is characterized by an increased level of CO2, absence of toxic compounds and the presence of hydrocarbons. Onto the serum protein fractions the salt mine speleotherapy cause a variation of their values depending on the pathology and used salt mine.

Experimental cure of speleotherapy in Ocna Dej and Cacica salt mines normalize hydric and electrolyte balance parameters in most cases (water and sodium intake, diuresis and renal sodium concentration ability) of rats with induced pathologies due to the direct effect of salt microclimate on the wounds and burns, and also due to saline load of the body in the presence of NaCl aerosol.


Induced pathology (burns, wounds, sensitization) does not cause a significant change in mineralocorticoid function of the adrenal glands regardless of animal age, instead speleotherapy cure lead to an increase in that, possibly due to a stimulation of the rennin-angiotensin--aldosterone system in Wistar white rats exposed to salt mines microclimate.

Hoteteu M (1), Simionca I (1), Munteanu C (1), Cinteza D (1), Lazarescu H (1), Dumitrescu E (1)

(1) National Institute of Rehabilitation, Physical Medicine and Balneology, Bucharest, Romania

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Title Annotation:POSTERS PAPERS: Biology; texto en ingles
Author:Hoteteu, M.; Simionca, I.; Munteanu, C.; Cinteza, D.; Lazarescum, H.; Dumitrescu, E.
Publication:Anales de Hidrologia Medica
Date:Dec 1, 2012
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