True branched nostocalean cyanobacteria from tropical aerophytic habitats and molecular assessment of two species from field samples.
The classification system proposed by Hoffmann et al. (2005) to Cyanobacteria merged the former Nostocales and Stigonematales into Nostocales, after several studies have pointed out that branching type has no phylogenetic value for distinguishing orders. The presence of heterocytes, however, supports phylogeny and indicates the monophyly of the group (Seo & Yokota 2003, Gugger & Hoffmann 2004). Further contributions to the classification of cyanobacteria (Komarek 2006, 2010) have supported the systematic arrangement proposed by Hoffmann et al. (2005) for this group of organisms.
The taxonomy of cyanobacteria is now based on polyphasic approach including morphological characters (e.g., cell division and polarity, branching type and thallus morphology), molecular data, ultrastructural arrangement (mainly thylakoids) and ecological parameters, which are combined to define groups. However, the developmental biology of the true branched cyanobacteria is still in the early stages of knowledge (Finsinger et al. 2008). The problems related to the artificial cultivation of most of true branched cyanobacteria samples (e.g. environmental exigencies not totally understood, slow growth and low competitive ability under artificial conditions prior to isolation) difficult the assessment of morphological variability and molecular sequencing by the most common and regular methods.
Taxonomic studies on true branched cyanobacteria are not abundant. The few papers devoted to the knowledge of these organisms include just some genera (e.g., Capsosira, Chlorogloeopsis, Fischerella, Nostochopsis, Stigonema and Westiellopsis) with reduced number of species, mainly about populations from temperate regions, and, in fact, this is considered not sufficient to solve the evolutionary history of the true branched cyanobacteria (Tomitani 2004).
Some of the most extensive surveys of tropical aerophytic cyanobacteria that include true branched taxa are those of Gardner (1927), Kufferath (1929), Golubic (1967), Hoffmann (1986), Sarthou et al. (1995), Budel et al. (1994, 1997, 2002), Coute et al. (1999), Pattanaik & Adhikary (2002) and Kastovsky et al. (2011). Surveys of the Brazilian flora of true branched cyanobacteria (including those from freshwater habitats) are very scarce and practically restricted to the papers of Silva & Sant'Anna (1991, 1996). The contributions of Branco & Necchi-Junior (1999) and Branco et al. (2010) focused single true branched freshwater species (Doliocatella formosa and Pulvinularia suecica, respectively) occurring in Brazilian territory.
This paper aims to improve the taxonomic knowledge of an ecologically and evolutionary important group of organisms and to describe morphological and habitat data of species present in a tropical region. In addition, the molecular assessment from field samples constitutes a complementary objective in order to improve the characterization of the populations found.
MATERIALS AND METHODS
Detailed information on sampling area, as well as on collection, preservation and characterization of sites, samples and taxonomic study are described in Branco et al. (2009). Briefly, samples of true branched cyanobacteria were collected in the rainforest region along the Northern littoral area of the Sao Paulo State (Table 1). Visible growths of cyanobacteria on soil, tree trunks and rocky substrates were randomly collected with spatula or penknife. The samples were stored in paper bags and dried at room temperature (usually for 24 to 48h). For each collection (sometimes different samples-coded SAMA-were taken from the same site), data about global position and altitude (GPS Garmin, eTrex Summit), air and plant-mass temperature (as close as possible to the growths) (digital thermometer with a 11cm long metal probe), air and plant-mass humidity (ThermoHygrometer Hana Instruments, HI8564), total and relative irradiance (Li-Cor Quantameter, with spherical sensor--the relative values are a relation between the absolute value measured as close as possible to the mass by the absolute value measured at an open area) and substratum pH (Merck pH strips-pH was measured through a solution with distilled/deionized water and a sample of the substratum) of collection sites and samples were recorded.
In the laboratory, samples were rehydrated and then examined under stereoscope (Olympus SZX7) and light (Olympus BX-50) microscopes and microphotographs were taken by using a digital camera (Olympus DP71) coupled to the light microscope. Specimens were studied according to their taxonomic features and were identified based on specific literature on cyanobacteria. The classification system by Hoffmann et al. (2005), with the modifications proposed by Komarek (2006), was adopted.
Field samples were selected for molecular analysis and the DNA extractions were made using the "Ultra Clean Microbial DNA Isolation" Kit (MoBio Laboratories). The products were amplified using the primers 27F1 and 1494Rc (Neilan et al. 1997) and the "Pure Taq Ready To-Go PCR Beads" kit (GE Life Science). Reactions were cycled with an initial denaturation step at 94[degrees]C for 4min, followed by 30 cycles of denaturation at 94[degrees]C for 20sec, annealing at 50[degrees]C for 30sec, extension at 72[degrees]C for 2min and final extension step at 72[degrees]C for 7min. PCR products were purified with Wizard PCR Preps DNA Purification System (Promega) and cloned by using the "pGEM[R]-T Easy Vector Systems" Kit (Promega). Vector with the insert was introduced by thermal shock (Sambrook et al. 1989) in competent Escherichia coli DH5[alpha] cells. Plasmids with the interested insert were purified by alkaline hydrolysis (Birnboim & Doly 1979) and amplified with "DYEnamic ET Terminator Cycle Sequencing" Kit (Amersham Biosciences, Pis cataway, NJ, EUA). M13F and SP6R vector primers and 357F, 357R, 704F, 704R, 1114F and 1114R internal primers (Lane, 1991) were used to select DNA sequences of about 1500bp.
Products were sequenced (in triplicates and both directions) in an ABI PRISM[R] 3100 Genetic Analyzer Applied Biosystems) and the nucleotide sequences obtained were aligned, refined and edited using the software BioEdit v 7.0.5 (Hall 1999). The two sequences produced in this study were compared to other 19 sequences of 16S rRNA gene retrieved from GenBank (NCBI), mostly representing taxonomically similar genera.
Phylogenetic trees were constructed by neighbor-joining (NJ; using SeaVIEW4 software), maximum likelihood (ML; PhyML 3.0) and Bayesian inference (BI; Mr. Bayes 3.12). Bootstrap values for neighbor-joining and maximum likelihood were generated on the basis of 1 000 replicates and Bayesian probability was based on two million generations. The evolution model used was GTR as suggested by Treefinder (version March 2011).
Environmental data: Values of the parameters were variable, but the ranges of species occurrence were mostly overlapped (Table 2). Values of pH ranged from 4-5, but reached up to 10; the values higher than five were observed for the lime painted hard substrates (rocks or cement curbs) where some populations were growing on. Total irradiance values oscillated from 300-2 740 [micro]mol photons/[m.sup.2]/s and the relative values (2-100%) showed the occurrence of populations in open to shaded sites. Air relative humidity was usually high, ranging from 60-92%, but always slightly lower than the values observed close to the plant masses (62-94%).
Floristic survey: Nine true branched nostocalean species, pertaining to three families, were found in the 20 samples from 15 distinct sites along the studied area (Table 1).
Spelaeopogon sommierii Borzi, Nuovo Giorn. Bot. Ital. 24: 108, 1917. (Fig. 1)
Plant mass formed by entangled filaments, caespitose, sometimes isolate filaments or in small groups in the mucilage of other cyanobacteria; main filament and branches similar; filaments usually uniseriate, occasionally biseriate, 10.0-11.0 [micro]m wide, irregularly T-branched, Y-false branching present; sheath thin, hyaline; trichomes constricted, sometimes not constricted, 8.0-10.0(-13.0) [micro]m wide; cells cylindrical to rounded, 10.0-12.0(-15.0) urn long; heterocytes intercalary or apical, 9.016.0 [micro]m long, (8.0-)10.0-12.0(-13.0) [micro]m wide. Occurrence: n=1; SAMA 09.
Hapalosiphon cf. aureus W. West & G.S. West, J. Bot. 35: 241, 1897. (Fig. 2)
Plant mass formed by entangled filaments, caespitose; main filament and branches similar or with slightly distinct in diameter; filaments uniseriate, 7.0-15.0(-17.5) [micro]m wide; branches usually on one side of the filament; sheath thin to moderately thick, hyaline to yellowish brown; trichomes constricted, 5.0-13.0 [micro]m wide; cells 4.0-10.0(-12.0) [micro]m long, 0.4-1.5 times longer than wide; cell content granulated, blue-green; heterocytes not abundant, 6.09.3um long, 5.5-6.8um wide.
Occurrence: n=1, SAMA 01.
Note: This population corresponds well to the description of Hapalosiphon aureus, but differs from it by its shorter cells in the branches and by its biotope characteristics. H. aureus is known from aquatic habitats, inhabiting standing water bodies, while the studied population is aerophytic on soil in tropical rainforest.
Hapalosiphon sp. (Fig. 3)
Plant mass formed by densely entangled filaments, prostrate; main filament and branches similar; filaments uniseriate, 6.0-9.0(10.7) um wide; branches long, usually on one side of the filament, not narrowing at the apex; sheath thin to moderately thick, hyaline to yellowish brown; trichomes constricted, 4.0-7.6 [micro]m wide; cells 3.5-7.0(-10.5) [micro]m long, 0.7-1.3(-2.3) times longer than wide; cell content granulated, pale green to pale blue-green; heterocytes frequent, cylindrical to sub-quadrate, 4.0-8.5(-10.0) [micro]m long, 5.0-8.0 [micro]m wide; arthrospores cylindrical to rounded, sometimes in long chains, usually with one (or more) big refringent granule (oil?) inside, 8.0-11.0 [micro]m long, 5.5-7.0 [micro]m wide, epispore thick, smooth, dark yellowish brown.
Occurrence: n=1, SAMA 45.
Note: this population resembles Hapalosiphon welwitschii in filament diameter, however heterocytes are rare and, mainly, the branches are short and tapered in this species.
Stigonema hormoides Bornet & Flahault, Ann. Sci. nat., 7, 5: 68, 1887. (Fig. 4)
Plant mass formed by entangled filaments, erect, yellowish brown or dark brown, or filaments grouped or growing isolated in gelatinous masses of other cyanobacteria; main filament and branches distinct; filaments with one row of cells, rarely two, (7.0-)9.0-17.5(22.0) [micro]m wide; branches usually short; sheath thick, yellowish to yellowish brown; cells variable, discoid, spherical, oval, squarish, rounded or irregular, 2.4-9.6(-14.0) [micro]m long, (-2.5)4.0-12.0 [micro]m wide; cell content granulated, blue-green; heterocytes hemispherical or rounded, lateral or intercalar, 4.0-9.6 [micro]m long, 3.2-9.6 [micro]m wide; homorgones terminal in the branches, 25.0-30.0(-70.0) [micro]m long.
Occurrence: n=9, SAMA 04, SAMA 22, SAMA 33, SAMA 34, SAMA 35, SAMA 36, SAMA 38, SAMA 45, SAMA 54.
Note: According to literature data, S. hormoides is distributed along tropical and temperate regions and its metrical and morphological ranges are relatively wide.
Stigonema informe Kutzing ex Bornet & Flahault, Ann. Sci. nat., 7, 5: 75, 1887. (Fig. 5)
Plant mass formed by entangled filaments, caespitose, yellowish to yellowish brown; main filament and branches distinct; main filaments with up to four rows of cells, 56.087.4 [micro]m wide; branches 24.0-44.0 [micro]m wide; sheath thick, yellowish to yellowish brown; cells hemispherical to rounded, sometimes grouped forming "packs", 7.2-16.0 [micro]m long, 6.4-12.0 [micro]m wide; cell content granulated, bluegreen; heterocytes hemispherical, lateral, 7.212.0 [micro]m long, 3.2-11.2 [micro]m wide; homorgones terminal in the branches, short, 16.0-32.0 [micro]m long, 6.9-12.0 [micro]m wide.
Occurrence: n=1, SAMA 38.
Stigonema minutum Bornet & Flahault, Ann. Sci. nat., 7, 5: 72, 1886. (Fig. 6)
Plant mass formed by entangled filaments, caespitose (main filament prostrate and branches erect); main filament and branches distinct; main filaments with up to three rows of cells, 20.0-28.0 [micro]m wide; branches up to two rows of cells, not enlarging or tapering towards to the end, 14.0-18.2(-20.0) [micro]m wide; sheath thick, yellowish to yellowish brown; cells discoid to hemispherical, 5.0-12.0(-15.0) [micro]m long, 5.0-13.0 [micro]m wide; cell content granulated, bluegreen; heterocytes hemispherical, lateral, 7.011.0 [micro]m long, 6.0-13.0 [micro]m wide; homorgones terminal in the branches, short.
Occurrence: n=4, SAMA 24, SAMA 36, SAMA 48, SAMA 54.
Note: The species is commonly referred in literature with distinct morphotypes. In addition, populations identified as S. minutum are recorded occurring in very distinct climate regions, from temperate (type material) to tropical or even polar areas. Species revision is probably needed.
Stigonema ocellatum Thuret ex Bornet & Flahault, Ann. Sci. nat., 7, 5: 69, 1887. (Fig. 7)
Plant mass formed by entangled filaments, erect or filaments grouped or isolated growing in gelatinous masses of other cyanobacteria; main filament and branches similar; filaments with one row of cells, occasionally two, 18.7-45.0 [micro]m wide; sheath thick, yellowish to yellowish brown; cells variable, irregular, hemispherical, oval, squarish or, more commonly, rounded, 3.0-20.0 [micro]m long, 4.025.6 [micro]m wide; cell content granulated, bluegreen; heterocytes discoid, hemispherical or rounded, lateral, 8.7-21.6 [micro]m long, 6.4-22.4 [micro]m wide; homorgones terminal in the branches, long at maturity, (20.0-)48.0-180.0 [micro]m long, 9.6-20.0nm wide.
Occurrence: n=12, SAMA 04, SAMA 10, SAMA 13, SAMA 22, SAMA 23, SAMA 33, SAMA 34, SAMA 35, SAMA 36, SAMA 37, SAMA 38, SAMA 53.
Note: This is one of the most commonly recorded species of the genus Stigonema and the literature data indicate a cosmopolitan distribution, occurring in very distinct types of habitats. As well as, morphological and metrical ranges of S. ocellatum are quite wide and some authors (Geitler, 1932) suggest that it is a species complex instead a well-defined taxonomic entity. More detailed studies, including molecular approaches, are surely required to a more precise evaluation.
Stigonema turfaceum Cooke ex Bornet & Flahault, Ann. Sci. nat., 7, 5: 74, 1887. (Fig. 8)
Plant mass formed by entangled filaments, caespitose; main filament and branches similar; filaments with up to four rows of cells, (20.0-)26.0-35.0(-40.0) [micro]m wide; branches slightly tapering to the apex; sheath thick, yellowish to yellowish brown; cells rounded, 4.8-12.5 [micro]m long, 4.0-11.0 [micro]m wide; cell content granulated, blue-green; heterocytes hemispherical, lateral; homorgones terminal, 9.5-45.0 [micro]m long, 4.0-11.0 [micro]m wide.
Occurrence: n=5, SAMA 02, SAMA 34,
SAMA 35, SAMA 36, SAMA 49.
Stigonema sp. (Fig. 9)
Isolated filaments growing mixed with other Stigonema species; main filament and branches similar; filaments with one row of cells, 8.0-12.8 [micro]m wide; sheath thick, smooth, hyaline; trichomes 4.0-7.2 [micro]m diam.; cells cylindrical, squarish, 2.4-8.0 [micro]m long.; cell content yellowish green, homogeneous; heterocytes rare, lateral, 3.1-3.5 [micro]m wide, 5.0-5.5 [micro]m long; hormogones not observed.
Occurrence: n=1; SAMA 38.
Note: This population is close to Stigonema hormoides, however the structure of the filament is distinct between them (cylindrical cells in the studied population and usually nearly spherical in S. hormoides). In addition, metrics are not fully compatible.
Molecular characterization: Sequencing was tried for all species surveyed, but positive results were obtained only for Hapalosiphon sp. (three clones, 1 390bp) and Stigonema ocellatum (one clone, 1 352bp). GenBank accession codes for the new sequences are GQ354274 and GQ354275, respectively.
Phylogenetic analysis on the basis of Hapalosiphon sp. and S. ocellatum sequences and 19 strain sequences retrieved from GenBank resulted in a consensus tree (Fig. 10) with different bootstrap/probability clade values according to the method employed.
The phylogenetic tree shows that both species grouped with heterocytous cyanobacteria, as expected. Stigonema ocellatum grouped with another strain of the same species (SAG 48.90, GenBank access code AJ544082, 98% sequence similarity) constituting a well-supported cluster (C1) with bootstrap/probability values of 89.2%, 100% and 1 (respectively ML, NJ and BI). The two S. ocellatum strains are close to a Nostoc commune strain.
Hapalosiphon sp. was similar to H. welwitschii (access code AY034793, 98% sequence similarity) and, according to NJ test (77% boot-strap), grouped with three other species of the genus (C2), but this group is not formed in ML and BI tests. However, a well-supported clade constituted by Hapalosiphon species and one strain of Westiellopsis prolifica (C3; cluster bootstrap/probability of 82.7%, 100% and 1 for ML, NJ and BI, respectively) was consistently formed in all tests performed. This Hapalosiphon/Westiellopsis clade is nested in a major group (C4; 77% and 0.96 for NJ and BI, respectively) that includes other morphologically similar genera (Fischerella, Nostochopsis and Chlorogloeopsis) spread in the clade.
Results on spatial distribution reveal that some species were well distributed throughout the sampling area (Stigonema ocellatum, 12 different samples, nine distinct sites; S. hormoides, nine samples, six sites), while some are geographically more restricted (Spelaeopogon somierii, Hapalosiphon cf. aureus, Hapalosiphon sp., Sigonema informe and Stigonema sp., all of them occurring in just one sample). The ecological data obtained, however, do not permit to address any conclusion on the species environmental preferences or tolerances. As most of measurements are punctual and too variable (e.g. light) or reasonably homogeneous in rainforest area (e.g. temperature, humidity), those data must be used as a reference for habitat characterization, but not as habitat definition. Nevertheless, the more widespread species occurred under wider ranges of variation for most parameters evaluated, as obviously expected.
All the species with occurrence in two or more samples were found growing on different substrate types (soil and rock) and substrate fidelity was not observed for any species recorded. Even though there are species with restricted dependence or preference on a determined substrate, frequently it is not possible to establish a clear relationship between the species and the substrate, especially for the most widespread and morphologically variable species. Unfortunately, there were not found studies in literature developing this subject in detail and more robust conclusions and comparisons cannot be addressed.
Interestingly, it is the very common joint occurrence of different species of Stigonema in a same sample or within a centimeter spatial scale. In sample SAMA 38, for instance, four species (S. hormoides, S. informe, S. ocellatum and Stigonema sp.) were found growing together. Stigonema hormoides, S. ocellatum and S. turfaceum were all present in the samples SAMA 34, SAMA 35 and SAMA 36. Thus, it is possible to speculate that many species have similar environmental requirements, but a more robust evaluation would only be supported with additional data from populations of the same and other ecologically distinct habitats.
Considering biogeography, only two species found, Stigonema hormoides and S. minutum, were also recorded by Hoffmann (1986) for Luxemburg and this low number of common species among the surveys is probably connected to the distinct climatic areas studied. In comparison with the data presented by Coute et al. (1999), for New Caledonia, five species (Stigonema hormoides, S. minutum, S. ocellatum, S. informe and S. turfaceum) were common to both studies, possibly reflecting environmental similarities among the areas. Sant'Anna et al. (1991) found, in a small cave, four species (Spelaeopogon sommierii, Stigonema hormoides, S. minutum and S. ocellatum) also recorded in this study and this relatively low similarity is surely due to the size of the area studied.
Although it is possible to reach specific identification for most of populations according to the classical monographs on cyanobacteria, the criteria used for species circumscription seem to be very wide. Many recorded species in literature perfectly correspond to original and further descriptions of such taxon, but more detailed analyses of illustrations reveal distinct filament structures and cell arrangements. However, the exact value of each taxonomic criterion used on this group is still to be proved, probably with the help of additional tools.
Some contributions to the cyanobacterial classification system (Komarek & Anagnostidis 2000, 2005, Hoffmann et al. 2005, Komarek 2006, 2010) emphasize the necessity of also taking into account the environmental and geographical characteristics in the identification process. If on one hand it is a well-supported concept because it probably reflects species requirements, it also creates a practical problem on the other. During this work, these premises were considered to improve the taxonomic evaluation but it is still very difficult to assure that two very similar (or identical) morphotypes are different because the existence of some ecological deviation from the type. The central problem is that it is still not clear if identical genotypes can support distinct environmental pressures without phenotypic changes or in what extension these changes occur. The concept of ubiquitous occurrence of most species has been discarded (Komarek 1994), but few data on genetic variability of true branched complex cyanobacteria have been produced to provide a solid theoretical background about the biogeography of the species.
The results of the molecular assessment obtained from this study suggest that populations identified as same species can be also similar at genetic level even they are from distinct climatic regions. The S. ocellatum SAG 48.90 is a cultivated strain from specimens collected in a Sphagnum peat bog in Germany and the paper of Gugger & Hoffmann (2004) presents a brief morphological account of the specimens. In general, there is good metrical correspondence and the identification as S.
ocellatum seems to be correct for all. However, the structure of filaments is not always quite similar (cell size in the main filament, cell size in branches, stratification), but it is difficult to evaluate if the variations are really meaningful. A critical review of different Stigonema ocellatum descriptions and illustrations found in literature (Fremy 1930, Geitler 1932, Skinner & Entwisle 2001, Komarek & Hauer 2011, this paper) corroborates that a very wide morphological range is recorded for the species, even though most of the populations can be correctly identified on the basis of the species original description. The present results from morphological and molecular studies and the available data in literature allow considering that S. ocellatum is probably a complex of species rather than a well delimited species. As it is a very widespread "species", more detailed molecular and morphological studies with populations from distinct geographical and ecological habitats are strongly recommended.
Hapalosiphon welwitschii AY034793, which is the closest sequence to Hapalosiphon sp., is a strain from Australian soils, but, unfortunately, no morphological data could be found for further comparison to the Brazilian material. The phylogenetic tree demonstrates that the clade formed by different species of Hapalosiphon, Westiellopsis, Fischerella and Nostochopsis constitute a mixed and much supported group and that the distinction among the different genera and species needs to be improved.
The molecular approach of the heterocytous true branched cyanobacteria group is still at the very initial stage. The usual techniques for DNA extraction require considerable amount of unicyanobacterial growths (cultures), that have been proved to be very uncommon to be achieved, mainly for species of Stigonema or Hapalosiphon, for instance. They apparently have a special set of environmental requirements to grow and their growth rate is usually low, favoring the establishment of cyanobacteria from other groups prior to the final isolation. New methods to study directly from nature have been developed (e.g. DNA sequencing from one or few cells), but they need to be improved and extended. Some time will be taken until we can access a reasonable amount of data to incorporate to the taxonomical approach. Meanwhile, it is important and relevant to record the morphological and molecular (when possible) diversity of populations with special geographical occurrence and ecology.
The cyanobacterial systematics is still confused and its reorganization on the basis of traditional morphological characters seems to be not adequate (Garcia-Pichel et al. 1996, Palinska et al. 1996). Instead, a polyphasic approach has been employed to better define the taxonomic levels (Zehr et al. 2003, Rajaniemi et al. 2005, Marquardt & Palinska 2007) and the modern taxonomy is becoming a synthetic science that incorporates several tools to achieve a more robust evaluation of the biodiversity. However, mainly in tropical areas, there is an enormous contingent of still not described morphotypes that is extremely relevant for the understanding of evolutionary relationships and producing a more natural classification system for this important group of organisms.
The authors thank to Conselho Nacional de Pesquisa e Desenvolvimento Tecnologico (CNPq), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) and the grant LH12100 to JK for the financial support. We also thank to TomaU Hauer (University of South Bohemia and Institute of Botany of Academy of Science of Czech Republic) for improving the molecular analysis and tree construction.
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Viviani Ferreira (1), Luis Henrique Z. Branco (1,2) & Jan Kastovsky (3)
(1.) Microbiology Graduation Program, UNESP-Sao Paulo State University, IBILCE, Sao Jose do Rio Preto, SP, Brazil; email@example.com
(2.) UNESP-Sao Paulo State University, IBILCE, Department of Zoology and Botany, R. Cristovao Colombo, 2265, BR-15054-000, Sao Jose do Rio Preto, SP, Brazil; firstname.lastname@example.org
(3.) University of South Bohemia, Department of Botany, Branisovska, 31a, 370 05, Ceske Budejovice, Czech Republic; email@example.com
TABLE 1 Global position and altitude of the collection sites and their respective samples Site Sample Latitude (S) Longitude (W) Altitude (m) 01 SAMA 01 23[degrees]35'51" 46[degrees]10'08" 800 02 SAMA 02 23[degrees]41'07" 46[degrees]05'52" 791 03 SAMA 04 23[degrees]41'09" 46[degrees]05'52" 787 04 SAMA 10 23[degrees]46'21" 45[degrees]57'14" 22 05 SAMA 13 23[degrees]46'14" 45[degrees]43'14" 55 08 SAMA 22 23[degrees]36'10" 45[degrees]26'43" 183 09 SAMA 09 23[degrees]30'34" 45[degrees]07'56" 0 10 SAMA 23, 23[degrees]35'16" 45[degrees]26'57" 600 SAMA 24 13 SAMA 33 23[degrees]32'56" 45[degrees]14'47" 23 14 SAMA 34, 23[degrees]29'39" 45[degrees]08'38" 66 SAMA 35, SAMA 36, SAMA 37 15 SAMA 38 23[degrees]23'06" 45[degrees]07'19" 122 18 SAMA 45 23[degrees]22'37" 45[degrees]07'07" 309 19 SAMA 48, 23[degrees]24'50" 45[degrees]01'43" 77 SAMA 49 21 SAMA 53 23[degrees]10'52" 44[degrees]50'00" 1 239 22 SAMA 54 23[degrees]09'54" 44[degrees]50'21" 1 460 TABLE 2 Values (or range) of selected ecological parameters of the sites where the species were found n AT MT ARH Spelaeopogon sommierii 1 * * * Hapalosiphon cf. aureus 1 19.6 19.9 85 Hapalosiphon sp. 1 22.3 21.5 92 Stigonema hormoides 9 21.1- 30.6 19.5-38.4 59-92 S. informe 1 24.6 25.5 80 S. minutum 4 21.9-27.7 19.5-36.0 55-86 S. ocellatum 12 19.6-31.0 18.3-38.0 62-83 S. turfaceum 5 21.8-27.7 20.0-23.9 74-90 Stigonema sp. 1 24.6 25.5 80 n MRH Ir Pir Spelaeopogon sommierii 1 * * * Hapalosiphon cf. aureus 1 90 110 29 Hapalosiphon sp. 1 94 43 66 Stigonema hormoides 9 67-94 35-2740 2-100 S. informe 1 87 160 51 S. minutum 4 62-89 14-1700 3-100 S. ocellatum 12 70-87 25-2740 5-100 S. turfaceum 5 75-93 3-330 5-48 Stigonema sp. 1 87 160 5 n pH S Spelaeopogon sommierii 1 * rock Hapalosiphon cf. aureus 1 5 soil Hapalosiphon sp. 1 5 soil Stigonema hormoides 9 4-5 rock, soil S. informe 1 5 rock S. minutum 4 4-5 rock, soil S. ocellatum 12 4-10 rock, soil S. turfaceum 5 4-5 rock, soil Stigonema sp. 1 5 rock * data not available. n=number of samples with species record, AT=air temperature ([degrees]C), MT=plant mass temperature ([degrees]C), ARH=relative humidity of the air (%), MRH=relative humidity close to the plant mass (%), Ir=irradiance measured close to the plant mass ([micro]mol photons/[m.sup.2]/s), PIr=percentage of irradiance reaching the plant mass in relation to the irradiance at a near open site (%), S=substratum type.
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|Author:||Ferreira, Viviani; Branco, Luis Henrique Z.; Kastovsky, Jan|
|Publication:||Revista de Biologia Tropical|
|Date:||Mar 1, 2013|
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