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Timeliness of yellow fever surveillance, Central African Republic.

Because the number of reported cases of yellow fever has increased over the past 2 decades, it is considered a reemerging disease (1,2). The World Health Organization (WHO) requires that all affected countries report yellow fever cases. Increasing risk for resurgence, potential severity of an epidemic, and possibility of preventing its spread by vaccination make early detection of yellow fever outbreaks essential, especially in a country such as the Central African Republic, where access to healthcare is difficult because of security concerns in several areas.

There are minimal data for yellow fever surveillance in Africa. The purpose of this study was to describe the timeliness, which was defined as the delay between date of onset of jaundice reported by the patient and date of an ELISA result, of the yellow fever surveillance system in the Central African Republic and identify temporal and spatial patterns and factors associated with delays in reporting.

The Study

This study was conducted as part of epidemiologic surveillance activities of the Ministry of Public Health of the Central African Republic. Data were obtained through the yellow fever surveillance system and approved by the Ministry of Health and WHO. All suspected cases reported during January 2007-July 2012 were included. A suspected case of yellow fever was defined as an acute onset of fever in a patient followed by jaundice within 2 weeks (3).

All blood samples from patients with suspected cases were tested at the Institut Pasteur (Bangui, Central African Republic) (IPB) for yellow fever virus-specific IgM by using the ELISA developed by the US Centers for Disease Control and Prevention (4). Positive samples were sent to the regional reference laboratory at the Institut Pasteur in Dakar, Senegal, where a plaque-reduction neutralization test (PRNT) was performed for case confirmation. A suspected yellow fever case was ruled out if ELISA or PRNT results were negative. When a suspected case was not ruled out by ELISA, health authorities were informed and an investigation was conducted so that vaccination could be implemented without delay if the case was confirmed.

The main study outcome was timeliness of the yellow fever surveillance system. Each intermediate step was studied: collection (from onset of jaundice to blood sample collection), field storage (from sample collection to shipping), transportation (from shipping to reception at IPB) and testing (from reception of sample to ELISA result).

Survival analysis was performed to determine how the following factors affected time to confirmed cases: age, sex, onset during the rainy season (May 1-October 31), province of residence, history of vaccination against yellow fever, and year of onset. Because the proportional hazard assumption was not verified for several variables, we used a parametric survival model and assumed a log-normal distribution of event times to estimate mutually adjusted time ratios. Subgroups were analyzed by confirmation status (confirmed or ruled out cases). Data were analyzed with Stata software version 11.0 (StataCorp LP, College Station, TX, USA) Maps were drawn with ArcGIS version 10.1 (Esri, Redlands, CA, USA).

During January 2007-July 2012, a total of 3,220 suspected cases of yellow fever were reported to IPB (online Technical Appendix Figure 1, article/20/6/13-0671-Techapp1.pdf). Suspected cases were reported in all provinces but mostly in Bangui (32.7%) and in neighboring Ombella M'Poko Province (24.7%) (online Technical Appendix Figure 2). Median age of patients with suspected cases was 21 years (interquartile range 10-30 years) and 57.5% were men. Only 21.9% reported having been vaccinated against yellow fever virus within the previous decade (Table 1).

Mean time to confirmation was 16.6 days (95% CI 16.2-16.9 days). Mean delay was 9.9 days (95% CI 9.510.2 days) for blood sample collection, 1.5 days (95% CI 1.4-1.6 days) for field storage, 1.1 days (95% CI 1.0-1.1 days) for transportation, and 4.2 days (95% CI 4.1-4.3 days) for testing. Detection of yellow fever (Figure 1, panel A) and delay for blood collection (Figure 1, panels B-E) were shortest in 2009.

Mean time until blood collection was shortest in Bangui (14.8 days) and surrounding areas and longest in Mbomou (26.2 days) (Figure 2, panel A). Mean delay for blood sample collection was shorter in eastern and northern regions of the country and longer in central and western regions (Figure 2, panel B). Mean length of transportation varied along a west-east gradient and ranged from 0.1 days in Bangui to 4.9 days in Haut Mbomou (Figure 2, panel D). A longer period to blood sample testing for the eastern part of the country was related to longer times of field storage and transportation (Figure 2, panels C, D). These areas are least accessible because of distance and security concerns. Transportation time was longer in the southwestern region despite its relative proximity to Bangui. Mean time for testing remained stable at [approximately equal to] 4-5 days (Figure 2, panel E).

Province of residence and year of onset were associated with a shorter period to diagnosis (p<0.001) (Table 2). Time to blood sample testing increased with patient age (p<0.001). No association was found between other characteristics and time required to confirm cases.

A total of 55 yellow fever cases were confirmed and 11 case-patients died. Of the confirmed case-patients, 22 (40.0%) were 15-24 years of age, 35 (63.6%) were male, and for 13 (23.6%) date of vaccination against yellow fever virus was unknown. Age and history of vaccination differed between patients who had confirmed yellow fever cases and those who did not (Table 1). Timelines were similar for confirmed and ruled out cases.


This study shows that in the Central African Republic, a country with limited health care and transportation facilities, confirmation of a yellow fever case takes 2-3 weeks. Approximately 10 days (60% of the delay) are required from the onset of symptoms to blood collection.

A fast time to case confirmation was observed in 2009, which was largely caused by a decrease in time required for blood collection, could be related to greater public awareness resulting from a yellow fever outbreak in Ombella M'Poko and Lobaye (5) and from a large outbreak of hepatitis E. This study showed an unexpected association between younger age and more rapid time to diagnosis, which was caused mainly by a shorter delay until blood collection, suggesting that parents have their children tested earlier. These 2 results indicate that the delay to blood collection could be reduced by better awareness of the population to the need for testing when they have symptoms compatible with yellow fever.

The 4-5 days of delay until testing is below the threshold of 7 days recommended by WHO. However, it could be decreased by implementation of a method of yellow fever detection (Bioplex Technology; BioRad Laboratories, Hercules, CA, USA), which is now in progress at IPB.

The need for confirmation by PRNT might delay vaccination because of time necessary for transportation of samples to Dakar and testing. The Central African Republic is landlocked and surrounded by countries to which yellow fever is endemic (6). Thus, PRNT for this region could be implemented at IPB, where biosafety level 3 facilities are functional. This factor could improve timeliness of diagnosis for rapid introduction of prevention measures.

Delays in reporting cases observed in this study are consistent with time between onset of symptoms of yellow fever in index case-persons and in persons infected by them, which is estimated to be -2 weeks (7). However, reasonable time for reporting is difficult to define. A comparison of delays found in this study with those in other countries would be helpful.


We thank the WHO office in Bangui for providing funds for transportation of samples to the laboratory and diagnostic reagents and the IPB for providing diagnostic tests.

Dr Rachas is an assistant professor at the European Hospital Georges Pompidou, Paris France. His primary research interest is the epidemiology of communicable diseases.


(1.) Gardner CL, Ryman KD. Yellow fever: a reemerging threat. Clin Lab Med. 2010;30:237-60.

(2.) Robertson SE, Hull BP, Tomori O, Bele O, LeDuc JW, Esteves K. Yellow fever: a decade of reemergence. JAMA. 1996;276:1157-62.

(3.) World Health Organization. WHO-recommended surveillance standard of yellow fever [cited 2013 Mar 25]. immunization_monitoring/diseases/YF_surveillance/en/index.html

(4.) World Health Organization. Manual for the monitoring of yellow fever virus infection. Geneva: The Organization; 2004.

(5.) World Health Organization. Yellow fever in the Central African Republic [cited 2012 Jul 20]. don/2009_12_01/en/print.html

(6.) Mayaux P, Bartholome E, Massart M, Van Cutsem C, Cabral A, Non-guierma A, et al. A land cover map of Africa. European Commission Joint Research Centre; 2003 [cited 2014 Mar 1]. http://bioval.jrc. ec.

(7.) Heymann DL, editor. Control of communicable diseases manual. 19th ed. Washington (DC): American Public Health Association; 2008.

Address for correspondence: Mirdad Kazanji, Institut Pasteur de Bangui, BP 923, Bangui, Central African Republic; email: mirdad.kazanji@

Antoine Rachas, [1] Emmanuel Nakoune, Julie Bouscaillou, Juliette Paireau, Benjamin Selekon, Dominique Senekian, Arnaud Fontanet, and Mirdad Kazanji

[1] Current affiliation: European Hospital Georges Pompidou and Descartes University, Paris, France.

Author affiliations: Institut Pasteur, Bangui, Central African Republic (A. Rachas, E. Nakoune, J. Bouscaillou, B. Selekon, M. Kazanji); Institut Pasteur, Paris, France (A. Rachas, J. Bouscaillou, J. Paireau, A, Fontanet); Ministry of Health, Bangui, (D. Senekian); and Conservatoire National des Arts et Metiers, Paris (A. Fontanet)


Table 1. Characteristics of 3,220 case-patients with suspected
yellow fever, Central African Republic, 2007-2012 *

Characteristic                          Suspected        Confirmed
                                      case-patients,   case-patients,
                                        n = 3,220          n = 55

Age, y
  <14                                  1,017 (31.7)       9 (16.4)
  15-24                                 882 (27.5)       22 (40.0)
  25-34                                 673 (21.0)       16 (29.1)
  [greater than or equal to] 35         633 (19.8)        8 (14.6)
  Median (IQR)                          21 (10-30)       23 (17-28)
  M                                    1,851 (57.5)      35 (63.6)
  F                                    1,366 (42.5)      20 (36.4)
Onset during rainy season
  No                                   1,446 (45.4)      27 (50.0)
  Yes                                  1,738 (54.6)      27 (50.0)
Province of residence
  Bangui                               1,053 (32.7)      11 (20.0)
  Ombella M'Poko                        794 (24.7)       30 (54.5)
  Lobaye                                189 (5.9)         5 (9.1)
  Sangha Mbaere                          20 (0.6)            0
  Mambere Kadei                          43 (1.3)            0
  Nana Mambere                           40 (1.2)            0
  Ouham Pende                           178 (5.5)         1 (1.8)
  Ouham                                 111 (3.5)            0
  Nana Grigbizi                          71 (2.2)            0
  Kemo                                   88 (2.7)            0
  Bamingui Bangoran                      23 (0.7)            0
  Ouaka                                 188 (5.8)         1 (1.8)
  Basse Kotto                           178 (5.3)         4 (7.3)
  Vakaga                                 33 (1.0)            0
  Haute Kotto                           114 (3.5)         3 (5.5)
  Mbomou                                 78 (2.4)            0
  Haut Mbomou                            19 (0.6)            0
Vaccination against yellow fever
  Never                                2,301 (71.5)      28 (50.9)
  >10 y ago                             160 (5.0)         3 (5.5)
  [less than or equal to] 10 y ago      705 (21.9)       11 (20.0)
  Unknown date                           54 (1.7)        13 (23.6)

Characteristic                          Ruled out      p value
                                        n = 3,165

Age, y
  <14                                  1,008 (32.0)      NA
  15-24                                 860 (27.3)       NA
  25-34                                 657 (20.9)       NA
  [greater than or equal to] 35         625 (19.8)      0.020
  Median (IQR)                          21 (10-31)      0.32
  M                                    1,816 (57.4)      NA
  F                                    1,346 (42.6)     0.36
Onset during rainy season
  No                                   1,419 (45.3)      NA
  Yes                                  1,711 (54.7)     0.50
Province of residence
  Bangui                               1,042 (32.9)      NA
  Ombella M'Poko                        764 (24.1)       NA
  Lobaye                                184 (5.8)        NA
  Sangha Mbaere                          20 (0.6)        NA
  Mambere Kadei                          43 (1.4)        NA
  Nana Mambere                           40 (1.3)        NA
  Ouham Pende                           177 (5.6)        NA
  Ouham                                 111 (3.5)        NA
  Nana Grigbizi                          71 (2.2)        NA
  Kemo                                   88 (2.8)        NA
  Bamingui Bangoran                      23 (0.7)        NA
  Ouaka                                 187 (5.9)        NA
  Basse Kotto                           174 (5.5)        NA
  Vakaga                                 33 (1.0)        NA
  Haute Kotto                           111 (3.5)        NA
  Mbomou                                 78 (2.5)        NA
  Haut Mbomou                            19 (0.6)        NC
Vaccination against yellow fever
  Never                                2,273 (71.8)      NA
  >10 y ago                             157 (5.0)        NA
  [less than or equal to] 10 y ago      694 (21.9)       NA
  Unknown date                           41 (1.3)      <0.001

* Values are no. (%) except as indicated. NA, not applicable;
IQR, interquartile range; NC, not computable.

Table 2. Factors associated with timeliness of yellow fever
surveillance, Central African Republic, 2007-2012 *

Factor                      Mean         Adjusted time      p value
                        timeliness, d    ratio (95% CI)    ([dagger])

Age, y

  <14                       15.3              1.00             NA
  15-24                     16.5        1.13 (1.08-1.18)       NA
  25-34                     16.8        1.15 (1.09-1.21)       NA
  [greater                  18.6        1.21 (1.15-1.27)     <0.001
    than or
    equal to]35


  M                         16.6              1.00             NA
  F                         16.5        1.01 (0.98-1.05)      0.43

Onset during
rainy season

  No                        16.7              1.00             NA
  Yes                       16.5        1.01 (0.97-1.04)       NA

Province of

  Bangui                    14.8              1.00             NA
  Ombella M'Poko            15.7        1.07 (1.02-1.12)       NA
  Lobaye                    16.8        1.18 (1.09-1.28)       NA
  Sangha Mbaere             17.6        1.17 (0.93-1.46)       NA
  Mambere Kadei             18.0        1.21 (1.03-1.41)       NA
  Nana Mambere              20.7        1.43 (1.22-1.67)       NA
  Ouham Pende               15.5        1.10 (1.01-1.19)       NA
  Ouham                     17.0        1.19 (1.08-1.32)       NA
  Nana Grigbizi             22.9        1.56 (1.39-1.77)       NA
  Kemo                      19.4        1.35 (1.21-1.51)       NA
  Bamingui Bangoran         17.5        1.24 (1.01-1.53)       NA
  Ouaka                     19.2        1.34 (1.24-1.46)       NA
  Basse Kotto               16.5        1.19 (1.10-1.29)       NA
  Vakaga                    20.1        1.38 (1.16-1.65)       NA
  Haute Kotto               17.4        1.25 (1.13-1.39)       NA
  Mbomou                    26.2        1.86 (1.66-2.09)       NA
  Haut Mbomou               23.9        1.72 (1.37-2.15)     <0.001

Vaccination against
yellow fever

  Never                     16.6              1.00             NA
  >10 y ago                 16.9        1.01 (0.93-1.09)       NA
  [less than or             16.3        1.01 (0.96-1.05)       NA
    equal to]10 y ago
  Unknown date              16.1        1.06 (0.92-1.23)      0.85

Year of onset

  2007                      17.8              1.00             NA
  2008                      17.3        1.02 (0.95-1.09)       NA
  2009                      14.7        0.91 (0.85-0.98)       NA
  2010                      16.8        1.04 (0.97-1.12)       NA
  2011                      17.8        1.10 (1.02-1.18)       NA
  2012                      16.6        1.05 (0.96-1.15)     <0.001

* Adjusted time ratios are from a parametric survival model assuming
log-normal distribution of the event times. A time ratio represents
a relative increase in time between 2 groups. Timeliness was defined
as the delay between the date of onset of jaundice (reported by the
patient) and the date of ELISA result. NA, not applicable.

([dagger]) By global Wald test.
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Article Details
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Title Annotation:DISPATCHES
Author:Rachas, Antoine; Nakoune, Emmanuel; Bouscaillou, Julie; Paireau, Juliette; Selekon, Benjamin; Seneki
Publication:Emerging Infectious Diseases
Geographic Code:6CENT
Date:Jun 1, 2014
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