The possible potential therapeutic targets for drug induced gingival overgrowth.
Drug-induced gingival overgrowth is an adverse drug reaction mainly described with three types of commonly prescribed drugs, namely, calcium channel blockers (nifedipine, diltiazem, and verapamil) [1-4], antiepileptic drugs (phenytoin)  and, immunosuppressants (cyclosporine) . Drug-induced gingival overgrowth usually occurs within the first 3 months of starting the medication and begins as an enlargement of the interdental papilla . As mentioned elsewhere, not all the patients taking these drugs develop drug-induced gingival overgrowth. While the incidence of this side effect can be as high as 65% in epileptics, 70% in transplant patients, and 30% in hypertension subjects, variation exists in the reported prevalence and severity of the clinical problem [7-10]. The degrees of inflammation, fibrosis, and cellularity depend on the duration, dose, and identity of the drug and on the quality of oral hygiene. Evidence suggests that genetic factors also might have a significant role in the pathogenesis of drug-induced gingival overgrowth and in the patient's susceptibility to this unwanted effect. A genetic predisposition could influence a variety of factors in the drug-plaque-induced inflammation. These include gingival fibroblast functional heterogeneity, collagenolytic activity, drug metabolism, and collagen synthesis [7,11]. The effective management of these patients clearly requires the active involvement of both dental and medical professionals to minimize the possibility of complications. The rationally designed antifibrotic therapies are likely to be invaluable in curbing this problem largely because the underlying basis of fibrosis is unclear. However, numerous studies conducted over three decades have suggested that chronic inappropriate increases in levels of local and circulating chemical mediators such as angiotensin II (Ang II) and endothelin-1 (ET-1) and fibrogenic cytokines/proteins such as transforming growth factor (TGF), connective tissue growth factor (CTGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), and mast cell chymase and tryptase enzymes are likely to be key driving forces culminating in gingival overgrowth (Figure 1). Collectively, these hormones and cytokines result in the activation of gingival fibroblasts cells within connective tissue. Although gingival fibroblasts are induced in response to normal tissue injury, these cells disappear, thereafter, probably by apoptosis. In DIGO, however, gingival fibroblasts persist, resulting in the excessive production and remodeling of extracellular matrix (ECM). Some studies demonstrated that drugs such as cyclosporine A are able to inhibit production of ECM by gingival fibroblast and cell proliferation in vitro . In contrast, others showed that the accumulation of proteins in ECM, particularly collagen, may occur due to an imbalance between the synthesis and the degradation of ECM, being the possible cause of the drug-induced gingival overgrowth . These results suggested the imbalance in the ECM synthesis and degradation that leads to drug-induced gingival overgrowth. The mechanisms that trigger drug-induced gingival overgrowth have not been completely understood, and, although the literature data are extensive, they are quite contradictory. Recent evidence has suggested that differentiation of resident fibroblasts occurs in response to above-mentioned mediators, which are all likely to play key roles in this process. In this review, we focused on growth factors such as, TGF-[beta], CTGF, IGF, and PDGF; mast cell enzymes chymase and tryptase; circulating mediators Ang II and ET-1, and discussed their roles in the pathogenesis of drug-induced gingival overgrowth.
2. Growth Factors
2.1. Transforming Growth Factor-[beta]. TGF-[beta] expression is elevated in response to injury . There is an extensive literature that discusses the basics of TGF-[beta] signaling and its relationship to fibrosis [15-17]. Briefly, there are 3 TGF-[beta] isoforms, namely, TGF-[beta] 1, TGF-[beta] 2, and TGF-[beta] 3. These are initially present within a complex containing latent TGF-[beta]-binding proteins that are proteolytically removed to release active TGF-[beta]. Substantial evidence supports a central role for TGF-[beta] in fibroblast activation. When applied to fibroblasts, TGF-[beta] directly induces ECM gene expression and promotes ECM deposition by simultaneously suppressing matrix metalloproteinase gene expression and inducing tissue inhibitors of matrix metalloproteinase gene expression [18-20]. It is well established that the immunosuppressive drug, cyclosporine, upregulates TGF-[beta] 1 synthesis, a fact that might explain the fibrogenic effect of this drug in a variety of cells and tissues [21-26]. Significantly, in vivo studies in animal models have shown that cyclosporine increases kidney TGF-[beta] 1 levels . Evidence from the study of isolated gingival fibroblasts in culture demonstrates that these cells exhibit a proliferative response to TGF-[beta] 1 [25-29]. Furthermore, hyper-responsiveness of gingival fibroblasts to autocrine effects of TGF-[beta] 1, coupled with increased levels of synthesis in these cells, may be important pathogenic elements in hereditary gingival fibromatosis, a condition that has histopathological features in common with drug-induced gingival overgrowth [30-32]. TGF-[beta] 1 has an important profibrogenic role, not only by inhibiting the synthesis of metalloproteinases but also by stimulating synthesis of collagen . In fibroblasts TGF-[beta] generally is signaled through TGF-[beta] type I receptor (Activin-Linked kinase 5) . Activin-Linked kinase 5 (ALK5) inhibitors have been considered as potential antifibrotic compounds, although these small molecules have not yet been thoroughly investigated in gingival fibroblasts. These results suggest that blocking ALK5 may be useful in blocking the profibrotic effects of TGF-[beta] in gingival fibroblasts. In gingival fibroblasts cells, ALK5 inhibition reduces profibrotic effect of TGF-[beta] . In addition to ALK5 inhibitors, anti-TGF-[beta] antibodies have also been under consideration as potential antifibrotic agents . All these findings suggest that broad targeting of TGF-[beta] ligand might not be a viable antifibrotic strategy, but ALK5 inhibition may be a useful approach.
2.2. Insulin-Like Growth Factor. Insulin-like-growth-factor (IGFs-) I and II are well known to play essential roles in significant biological activities such as proliferation, differentiation, apoptosis, and adaptation in various kinds of tissues . The actions of IGFs are mainly mediated via IGF receptor 1 (IGFR-1) and are modulated by six binding proteins (IGFBPs), designated as IGFBP-1 to IGFBP-6 [35, 36]. Unlike most of the other binding proteins, which act as competitive inhibitors of IGF-I receptor, IGFBP-5 acts to enhance IGF-I actions. IGF-I increases the synthesis of both IGFBP-5 and collagen . Moreover, IGF-I's biological activity on fibroblasts includes stimulation of collagen production and downregulation of collagenase production, suggesting that IGF-I may be an important mediator in the development of gingival fibrosis through IGFBP-5 . The greater expression of IGFBP-5 in periodontal ligament fibroblast (PDLF) together with IGF-I induced reduction of apoptosis in PDLF suggests a potential role of IGFBP-5 in the upregulation of IGF-I pathway . Furthermore, augmented expressions of IGFBP-5 were identified in cyclosporine-induced gingival overgrowth and associated with increased collagen and fibrosis . It also demonstrated that, in rat gingival cells, the cyclosporine triggers IGF-I induced proliferation and extracellular matrix synthesis . IGF-I receptor inhibitors have been considered as potential target, and IGF-I receptor antagonist (A12) administration decreased lung fibrosis in bleomycin-treated mice . IGF-I receptor antagonist has been identified as possible new therapeutic approaches in kidney, lung, and cardiac fibrosis, but it has not yet been investigated thoroughly in gingival fibrosis.
2.3. Platelet-Derived Growth Factor. PDGF comprises a family of homo- or heterodimeric growth factors including PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGFDD. There are 2 different PDGF receptors, [alpha] and [beta] . PDGF causes neutrophils, macrophages, fibroblasts, and smooth muscle cells proliferation and migration [43, 44]. PDGF-B expression has been shown to occur early in gingival wound healing  and is increased in cyclosporine treatment . The PDGF-BB isoform is also known to cause proliferation of periodontal fibroblasts and modulate their production of extracellular matrix components . It has been demonstrated that many cell types within the overgrown gingival tissues express PDGF-A, PDGF-B, and both PDGF-receptors and are in agreement with the fact that PDGF is produced by a variety of cell types [48, 49]. Both phenytoin and cyclosporine cause increased production of PDGF AB/BB by macrophages in vitro [48, 50]. Furthermore, limited analysis of both control and cyclosporine-induced overgrowth tissues by immunohistochemistry and in situ hybridisation revealed that PDGF-B expression was restricted to some of the CD51-positive macrophages within the papillary lamina propria [49, 51]. Taken together these findings support a role for PDGF in the pathogenesis of gingival overgrowth. It has also been shown that injection of anti-PDGF-B antibodies reduces liver fibrosis , kidney fibrosis , and atrial fibrosis . Although there was no specific published data available on gingival fibrosis, there is substantial evidence strongly suggesting that PGDF may be a good target for antifibrotic therapy in drug-induced gingival overgrowth.
2.4. Connective Tissue Growth Factor. CTGF or CCN2, is a 38 kDa secreted protein belonging to the CCN family of growth factors [55, 56]. CCN2/CTGF has been shown to promote the synthesis of various constituents of the extracellular matrix [56, 57], and its overexpression is associated with the onset and progression of fibrosis in human gingiva [58, 59]. CCN2 promotes cell adhesion and enhances adhesive signaling in response to extracellular ligands. CCN2 is an excellent surrogate marker for activated fibroblasts in fibrosis; for example, in the process of gingival overgrowth, connective tissue growth factor (CTGF/CCN2) is significantly induced in gingival fibroblasts . Furthermore, CTGF/CCN2 is expressed in gingival epithelial cells in vivo in fibrotic tissues but not in normal tissues . CTGF is rapidly and potently induced by TGF-[beta] 1 in fibroblastic cells from a variety of different tissues and contributes to the regulation of extracellular matrix genes . However, on its own, CCN2 is considered to only weakly promote fibrosis; rather, what CCN2 appears to do is to create an environment favorable for fibrogenic stimuli to act [61, 62]. Levels of connective tissue growth factor (CTGF/CCN2) are highest in gingival tissues from phenytoin-induced lesions, intermediate in nifedipine-induced lesions, and nearly absent from cyclosporine-A-induced overgrowth. CTGF/CCN2 levels correlate positively with fibrosis, consistent with the role of CTGF/CCN2 in promoting and maintaining fibrosis . CTGF expression is related to the degree of fibrosis, as cyclosporine tissues show little CTGF expression and were demonstrated to be highly inflamed and not fibrotic.
Drugs targeting the action of CCN2, such as small interfering RNAs or neutralizing antibodies, are currently under development . A CCN2 response element exists in the COL1A2 promoter, and recently it has been shown that blocking CCN2 action using an anti-CCN2 antibody or small interfering RNA reduces aspects of bleomycin-induced lung fibrosis . Overall, strong in vivo evidence directly supporting the notion of anti-CCN2 therapies in pathologies of gingival over growth is lacking. However, it is possible that CCN2 may be a key selective modulator in the gingival overgrowth, operating downstream and in concert with TGF-[beta].
3. Mast Cell Proteases
Mast cells are derived from precursor cells in the bone marrow and mature under the influence of the c-kit ligand, stem cell factor with their final phenotype being dependent on the microenvironment in which they reside. While mast cells throughout the body are involved in the pathogenesis of many diseases, the role of gingival mast cells in gingival overgrowth has been understudied despite circumstantial evidence indicating their potential involvement. In addition to the storage of histamines and proteases such as tryptase and chymase in their secretory granules, gingival mast cells also produce a wide variety of cytokines, growth factors, and other biologically active mediators that are capable of mediating tissue remodeling. Several in vitro studies have pointed out the potential role of mast cells inducing gingival fibroblast proliferation and collagen synthesis . A recently published study demonstrated that mast cell tryptase and chymase enzymes were expressed significantly higher in DIGO tissues compared to healthy gingiva and may contribute to alterations in basement membranes . In addition, tryptase can interact with a number of cell types and has been shown to be mitogenic for fibroblasts  and epithelial cells . The immunohistochemical expression of mast cell tryptase in inflammatory fibrous gingival hyperplasia demonstrated the involvement of mast cells in the induction of fibrosis by observing increased mast cell degranulation in fibrous tissues . Mast cell chymase-positive gingival overgrowth patients showed significantly increased fibrotic lesions in the gingival tissues compared with chymase-negative patients .
Ang II, which is generated through angiotensinconverting enzyme (ACE), stimulates growth of the gingival tissue. However, an alternative generation pathway, chymase, also exists in the gingiva, and chymase has a higher specificity for the conversion of Ang I to Ang II. In addition, mast cells store latent transforming-growth-factor-(TGF-) [beta] in an intracellular granule and secrete TGF-[beta], which is activated by chymase . Locally synthesized chymase seems to promote gingival remodeling via the formation of Ang II and proliferative properties. Moreover, mast cells are likely to contribute indirectly to the formation of ET-1 by releasing chymase, which can generate ET-1, by cleavage of big ET-1 . ET-1 is a powerful vasoconstrictor with mitogenic activity on gingival fibroblasts and facilitates gingival fibrosis . Thus, the role of mast cells in the generation of fibrosis involves the direct effect of mast cell-derived ET-1 and Ang II, the exacerbation of preexisting inflammatory mediators, and the release of mast cell enzymes, such as tryptase and chymase.
The chymase and tryptase inhibitors have been considered as potential antifibrotic compounds although these inhibitors have not yet been thoroughly investigated in drug-induced gingival overgrowth. In skin mast cells, the selective inhibitor of chymase ZIGPFM inhibited histamine release and proliferation; moreover, with chymostatin, which is also a potent inhibitor of chymase, inhibition of 70 or 80% of mast cell mediators was achieved . It has been demonstrated that in cardiac fibrosis the chronic chymase inhibition prevented cardiac remodeling and fibrosis [75,76]. The similar degree of inhibition was observed with tryptase inhibitor APC366 and with certain other inhibitors of tryptic proteinases . These results suggest that inhibitors of chymase and tryptase canbe potent stabilizers ofhuman mast cells and particularly those in relation to overgrown gingival tissues. It is possible that the development of potent and selective chymase/tryptase inhibitors will lead to useful new drugs for the treatment of mast cell-mediated drug-induced gingival overgrowth.
4. Angiotensin II
Elevated intragingival Ang II production is found in drug-induced gingival overgrowth . Angiotensin is an oligopeptide that causes vasoconstriction and increased blood pressure. Although numerous factors have been implicated in the pathogenesis of drug-induced gingival overgrowth, several lines of evidence strongly suggest the involvement of Ang II in the development of DIGO  Figure 2). Ohuchi et al.  have reported that Ang II generated by gingival fibroblasts in response to phenytoin and nifedipine may contribute to the development of drug-induced gingival overgrowth. Recent studies indicate that Ang II and TGF-[beta] 1 do not act independently from one another but act as part of an integrated signaling network that promotes cardiac remodeling and possibly fibrosis. Ang II upregulates TGF-[beta] 1 expression through the angiotensin type 1 (AT1) receptor in gingival fibroblasts . In an important study, it was shown that Ang II was not able to induce fibrosis in vivo in the absence of TGF-[beta] . Providing further support for this notion, Ang II induces collagen in gingival fibroblasts through TGF-[beta] and ERK [81, 82]. Ang II and TGF-[beta] 1 appear to act in an autocrine loop, as TGF-[beta] 1 can directly stimulate AT1 receptor expression through ALK5 and Smads 2/3/4, providing a further indication of crosstalk between the TGF-[beta] and angiotensin pathways . These results indicate that Ang II and TGF-[beta] pathways are likely to cooperate to drive fibrogenic responses in vivo. Drugs that inhibit the angiotensin pathway, namely, angiotensin-converting enzyme inhibitors and angiotensin receptor antagonists, are widely used to treat various fibroses. Angiotensin receptor inhibitors such as losartan appear to be effective in reducing gingival fibrosis . Hilgers and Mann  demonstrated that treatment with an ACE inhibitor or AT1 receptor antagonist abrogates drug-induced hypertrophy. Further clinical studies showed that treatment with ACE inhibitor or AT1 receptor antagonist reduced fibrogenesis in CsA-treated patients after renal transplantation [85, 86]. Thus, compared to generally antagonizing TGF-[beta] signaling, angiotensin-converting enzyme inhibitors and angiotensin receptor antagonists may be useful approaches to control gingival overgrowth.
Endothelin is a protein secreted from endothelial cells, and it is a powerful vasoconstrictor with mitogenic or comitogenic properties, which acts through the stimulation of 2 subtypes of receptors (ETA and ETB) [87, 88]. ET-1 is first produced in the form of a 212-aa precursor (prepro-ET-1) and then cleaved twice to form a biologically active 21-aa peptide, the last cleavage mediated by ET-converting enzyme . Studies have shownthe expressionofET-1ininflamed gingival tissues and threefold increase in drug-induced gingival overgrowth tissues  Teder and Noble,  also found that ET-1 was localized in inflamed gingival epithelial cells. Similar results found that ET-1 was increased in cardiac, renal, and gingival fibrosis [89-91]. The increased ET-1 can modulate the synthesis of TGF-[beta] and TNF-[alpha], which are important in mediating fibroses. Our previous study demonstrated the augmented expression of TNF-a in drug-induced gingival overgrowth tissues compared to healthy gingival tissues . ET-1 is also profibrotic by stimulating fibroblast replication, migration, and contraction, increasing fibronectin, collagen synthesis, and decreasing collagen degradation [93-96]. All of these processes have been proposed for the pathogenesis of DIGO (Figure 3). Ohuchi et al.  showed that phenytoinand nifedipine-induced gingival fibroblasts proliferation was mediated through the induction of Ang II and ET-1 and probably mediated through Ang II receptor-1 and ETA in gingival fibroblasts. Some studies have suggested that TGF-[beta] works together with ET-1 to promote myofibroblast differentiation . TGF-[beta] induces ET-1 via JNK, and ET-1 is a downstream mediator of the fibrogenic responses of TGF-[beta] in normal fibroblasts . Intriguingly, Ang II also induces ET-1 via ERK and reactive oxygen species . These results suggest that ET operates downstream of the TGF-[beta]/Ang II system to drive fibroblast activation and fibrosis.
ET receptor antagonism might be considered as an appropriate therapy for the fibrosis. Recent data demonstrate the fundamental role of endothelin in the pathogenesis of fibrosis and the antifibrotic potential of dual endothelin receptor antagonists such as bosentan . It is an orally active, dual endothelin receptor antagonist, which competitively antagonizes the binding of endothelin to both endothelin receptors ETA and ETB, and prevents endothelin-induced fibroblast proliferation and extracellular matrix deposition and contraction, and reduces fibrosis . Bosentan has already been approved by the Food and Drug Administration for the treatment of pulmonary arterial hypertension [99, 100]. Furthermore, macitentan is an orally active, nonpeptide dual endothelin ETA and ETB receptor antagonists for the potential treatment of idiopathic pulmonary fibrosis and pulmonary arterial hypertension . Since it has the ability to target the tissues and to blockboth ETA and ETB receptors, macitentan is emerging as a new agent to treat cardiovascular disorders associated with chronic tissue ET system activation . It is interesting to note that dual-acting angiotensin II and ET receptor blockers have been shown to reduce systemic blood pressure in animal models and in hypertensive patients [102, 103]. Preliminary data in smaller human studies have shown that these agents are safe and well tolerated [102, 103]. Thus combination ET/Ang II therapies have promise in controlling gingival overgrowth.
Drugs that modulate TGF-[beta], PDGF, CTGF, IGF, mast cell chymase/tryptase, Ang II, and ET-1 are being considered for protective gingival overgrowth effects and can ameliorate fibrosis. Even though these proteins are likely to cooperate in driving tissue remodeling and fibrogenic responses in fibroblasts, each protein also has certain unique features, providing a rationale that therapies targeting individual molecules might be useful. Current strategies in clinical practice combine treatments with these inhibitors and receptor blockers, due to their potential additive beneficial effects. The data presented here highlight potential interesting candidates for antifibrotic treatments. Future studies are necessary to evaluate their potential beneficial effects fully in gingival overgrowth.
 R. A. Seymour, "Calcium channel blockers and gingival overgrowth," British Dental Journal, vol. 170, no. 10, pp. 376-379, 1991.
 C. S. Miller and D. D. Damm, "Incidence of verapamil-induced gingival hyperplasia in a dental population," Journal of Periodontology, vol. 63, no. 5, pp. 453-456, 1992.
 S. Nishikawa, T. Nagata, I. Morisaki, T. Oka, and H. Ishida, "Pathogenesis of drug-induced gingival overgrowth. A review of studies in the rat model," Journal of Periodontology, vol. 67, no. 5, pp. 463-471, 1996.
 J. S. Ellis, R. A. Seymour, J. G. Steele, P. Robertson, T. J. Butler, and J. M. Thomason, "Prevalence of gingival overgrowth induced by calcium channel blockers: a community-based study," Journal of Periodontology, vol. 70, no. 3, pp. 63-67, 1999.
 F. Perlik, M. Kolinova, J. Zvarova, and V Patzelova, "Phenytoin as a risk factor in gingival hyperplasia," Therapeutic Drug Monitoring, vol. 17, no. 5, pp. 445-448, 1995.
 T McGaw, S. Lam, and J. Coates, "Cyclosporin-induced gingival overgrowth: correlation with dental plaque scores, gingivitis scores, and cyclosporin levels in serum and saliva," Oral Surgery Oral Medicine and Oral Pathology, vol. 64, no. 3, pp. 293-297, 1987.
 T. M. Hassell and A. F. Hefti, "Drug-induced gingival overgrowth: old problem, new problem," Critical Reviews in Oral Biology and Medicine, vol. 2, no. 1, pp. 103-137, 1991.
 R. M. Lucas, L. P. Howell, and B. A. Wall, "Nifedipine-induced gingival hyperplasia. A histochemical and ultrastructural study," Journal of Periodontology, vol. 56, no. 4, pp. 211-215, 1985.
 S. Barclay, J. M. Thomason, J. R. Idle, and R. A. Seymour, "The incidence and severity of nifedipine-induced gingival overgrowth," Journal of Clinical Periodontology, vol. 19, no. 5, pp. 311-314, 1992.
 J. M. Thomason, "Determinants of gingival overgrowth severity in organ transplant patients. An examination of the role of HLA phenotype," Journal of Clinical Periodontology, vol. 23, no. 7, pp. 628-634, 1996.
 T. M. Hassell, R. C. Page, A. S. Narayanan, and C. G. Cooper, "Diphenylhydantoin (dilantin) gingival hyperplasia: drug induced abnormality of connective tissue," Proceedings of the National Academy of Sciences of the United States of America, vol. 73, no. 8, pp. 2909-2912, 1976.
 T. Modeer, I. Anduren, A. Bengtsson, and G. Andersson, "Interleukin-1 beta and phenytoin reduce alpha 1 procollagen mRNA expression in human gingival fibroblasts," Journal of Periodontal Research, vol. 31, no. 8, pp. 563-569, 1996.
 L. C. Spolidorio, H. F. G. Gonzaga, and D. M. P. Spolidorio, "Quantitative analysis of gingival tissues of rats treated with phenytoin and cyclosporine," Pesquisa Odontologica Brasileira, vol. 14, pp. 327-333, 2000.
 C. J. M. Kane, P. A. Hebda, J. N. Mansbridge, and P. C. Hanawalt, "Direct evidence for spatial and temporal regulation of transforming growth factor [beta]1 expression during cutaneous wound healing," Journal of Cellular Physiology, vol. 148, no. 1, pp. 157-173, 1991.
 J. Massague, "TGF-beta signal transduction," Annual Review of Biochemistry, vol. 67, pp. 753-791, 1998.
 A. Leask and D. J. Abraham, "TGF-beta signaling and the fibrotic response," The FASEB Journal, vol. 18, no. 7, pp. 816-827, 2004.
 A. B. Roberts, "TGF-beta signaling from receptors to the nucleus," Microbes and Infection, vol. 1, no. 15, pp. 1265-1273, 1999.
 M. H. Branton and J. B. Kopp, "TGF-beta and fibrosis," Microbes and Infection, vol. 1, no. 15, pp. 1349-1365, 1999.
 O. Eickelberg, "Endless healing: TGF-[beta], SMADs, and fibrosis," FEBS Letters, vol. 506, no. 1, pp. 11-14, 2001.
 P. J. Sime and K. M. A. O'Reilly, "Fibrosis of the lung and other tissues: new concepts in pathogenesis and treatment," Clinical Immunology, vol. 99, no. 3, pp. 308-319, 2001.
 B. Li, P. K. Sehajpal, A. Khanna et al., "Differential regulation of transforming growth factor [beta] and interleukin 2 genes in human T cells: demonstration by usage of novel competitor DNA constructs in the quantitative polymerase chain reaction," Journal of Experimental Medicine, vol. 174, no. 5, pp. 1259-1262, 1991.
 A. Khanna, B. Li, K. H. Stenzel, and M. Suthanthiran, "Regulation of new DNA synthesis in mammalian cells by cyclosporine: demonstration of a transforming growth factor [beta]-dependent mechanism of inhibition of cell growth," Transplantation, vol. 57, no. 4, pp. 577-582, 1994.
 M. Shehata, G. H. Cope, T. S. Johnson, A. T. Raftery, and A. M. El Nahas, "Cyclosporine enhances the expression of TGF-[beta] in the juxtaglomerular cells of the rat kidney," Kidney International, vol. 48, no. 5, pp. 1487-1496, 1995.
 G. Wolf, F. Thaiss, and R. A. K. Stahl, "Cyclosporine stimulates expression of transforming growth factor-in renal cells: possible mechanism of cyclosporines antiproliferative effects," Transplantation, vol. 60, no. 3, pp. 237-241, 1995.
 A. K. Khanna, V R. Cairns, C. G. Becker, and J. D. Hosenpud, "TGF-[beta]: a link between immunosuppression, nephrotoxicity, and CsA," Transplantation Proceedings, vol. 30, no. 4, pp. 944-645, 1998.
 A. Khanna, V. Cairns, and J. D. Hosenpud, "Tacrolimus induces increased expression of transforming growth factor- [beta]1 in mammalian lymphoid as well as nonlymphoid cells," Transplantation, vol. 67, no. 4, pp. 614-619, 1999.
 D. K. Dennison, D. R. Vallone, G. J. Pinero, B. Rittman, and R. G. Caffesse, "Differential effect of TGF-[beta]1 and PDGF on proliferation of periodontal ligament cells and gingival fibroblasts," Journal of Periodontology, vol. 65, no. 7, pp. 641-648, 1994.
 T. J. Anderson, C. A. Lapp, M. A. Billman, and G. S. Schuster, "Effects of transforming growth factor-[beta] and platelet-derived growth factor on human gingival fibroblasts grown in serum-containing and serum-free medium," Journal of Clinical Periodontology, vol. 25, no. 1, pp. 48-55, 1998.
 J. A. James, C. R. Irwin, and G. J. Linden, "Gingival fibroblast response to cyclosporin A and transforming growth factor [beta]1," Journal of Periodontal Research, vol. 33, no. 1, pp. 40-48, 1998.
 D. A. Tipton and M. K. Dabbous, "Autocrine transforming growth factor [beta] stimulation of extracellular matrix production by fibroblasts from fibrotic human gingiva," Journal ofPeriodontology, vol. 69, no. 6, pp. 609-619, 1998.
 R. D. Coletta, O. P. Almeida, M. A. Reynolds, and J. J. Sauk, "Alteration in expression of MMP-1 and MMP-2 but not TIMP1 and TIMP-2 in hereditary gingival fibromatosis is mediated by TGF-[beta]1 autocrine stimulation," Journal of Periodontal Research, vol. 34, no. 8, pp. 457-463, 1999.
 C. R. de Andrade, P. Cotrin, E. Graner, O. P Almeida, J. J. Sauk, and R. D. Coletta, "Transforming growth factor-[beta]1 autocrine stimulation regulates fibroblast proliferation in hereditary gingival fibromatosis," Journal of Periodontology, vol. 72, no. 12, pp. 1726-1733, 2001.
 T. Kisseleva and D. A. Brenner, "Mechanisms of fibrogenesis," Experimental Biology and Medicine, vol. 233, no. 2, pp. 109-122, 2008.
 K. Thompson, D. W Hamilton, and A. Leask, "ALK5 inhibition blocks TGF[beta]-induced CCN2 expression in gingival fibroblasts," Journal of Dental Research, vol. 89, no. 12, pp. 1450-1454, 2010.
 C. E. H. Stewart and P. Rotwein, "Growth, differentiation, and survival: multiple physiological functions for insulin-like growth factors," Physiological Reviews, vol. 76, no. 4, pp. 1005-1026, 1996.
 J. I. Jones and D. R. Clemmons, "Insulin-like growth factors and their binding proteins: biological actions," Endocrine Reviews, vol. 16, no. 1, pp. 3-34, 1995.
 X. Xin, Y. T. Hou, L. Li et al., "IGF-I increases IGFBP-5 and collagen [alpha]1(I) mRNAs by the MAPK pathway in rat intestinal smooth muscle cells," American Journal of Physiology, vol. 286, no. 5, pp. G777-G783, 2004.
 X. Han and S. Amar, "IGF-1 signaling enhances cell survival in periodontal ligament fibroblasts vs. gingival fibroblasts," Journal of Dental Research, vol. 82, no. 6, pp. 454-459, 2003.
 T. Subramani, A. Sakkarai, K. Senthilkumar, S. Periasamy, G. Abraham, and S. Rao, "Expression of insulin like growth factor binding protein-5 in drug induced human gingival overgrowth," Indian Journal of Medical Research, vol. 125, no. 1, pp. 43-48, 2007.
 T. Yoshida, J. Nagata, and A. Yamane, "Growth factors and proliferation of cultured rat gingival cells in response to cyclosporin A," Journal of Periodontal Research, vol. 40, no. 1, pp. 11-19, 2005.
 J. E. Choi, S. S. Lee, D. A. Sunde et al., "Insulin-Like growth factor-i receptor blockade improves outcome in mouse model of lung injury," American Journal of Respiratory and Critical Care Medicine, vol. 179, no. 3, pp. 212-219, 2009.
 J. C. Bonner, "Regulation of PDGF and its receptors in fibrotic diseases," Cytokine and Growth Factor Reviews, vol. 15, no. 4, pp. 255-273, 2004.
 C. H. Heldin and B. Westermark, "Mechanism of action and in vivo role of platelet-derived growth factor," Physiological Reviews, vol. 79, no. 4, pp. 1283-1316, 1999.
 M. Uutela, M. Wirzenius, K. Paavonen et al., "PDGF-D induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis," Blood, vol. 104, no. 10, pp. 3198-3204, 2004.
 R. J. Green, M. L. Usui, C. E. Hart, W. F. Ammons, and A. S. Narayanan, "Immunolocalization of platelet-derived growth factor A and B chains and PDGF-[alpha] and [beta] receptors in human gingival wounds," Journal of Periodontal Research, vol. 32, no. 2, pp. 209-214, 1997
 M. Shehata, A. M. El Nahas, E. Barkworth, G. H. Cope, and A. T. Raftery, "Increased platelet-derived growth factor in the kidneys of cyclosporin-treated rats," Kidney International, vol. 46, no. 3, pp. 726-732, 1994.
 J. M. Mailhot, G. S. Schuster, J. J. Garnick, P. J. Hanes, C. A. Lapp, and J. B. Lewis, "Human periodontal ligament and gingival fibroblast response to TGF-beta 1 stimulation," Journal of Clinical Periodontology, vol. 22, no. 9, pp. 679-685, 1995.
 R. E. Dill, E. K. Miller, T Weil, S. Lesley, G. R. Farmer, and A. M. Iacopino, "Phenytoin increases gene expression for platelet-derived growth factor B chain in macrophages and monocytes," Journal of Periodontology, vol. 64, no. 3, pp. 169-173, 1993.
 J. M. Plemons, R. E. Dill, T. D. Rees, B. J. Dyer, M. C. Ng, and A. M. Iacopino, "PDGF-B producing cells and PDGF-B gene expression in normal gingiva and cyclosporine A-induced gingival overgrowth," Journal of Periodontology, vol. 67, no. 3, pp. 264-270, 1996.
 A. M. Iacopino, D. Doxey, C. W. Cutler et al., "Phenytoin and cyclosporine A specifically regulate macrophage phenotype and expression of platelet-derived growth factor and interleukin-1 in vitro and in vivo: possible molecular mechanism of drug-induced gingival hyperplasia," Journal of Periodontology, vol. 68, no. 1, pp. 73-83, 1997
 S. Nares, M. C. Ng, R. E. Dill, B. Park, C. W. Cutler, and A. M. Iacopino, "Cyclosporine A upregulates platelet-derived growth factor B chain in hyperplastic human gingiva," Journal of Periodontology, vol. 67, no. 3, pp. 271-278, 1996.
 S. Ogawa, T Ochi, H. Shimada et al., "Anti-PDGF-B monoclonal antibody reduces liver fibrosis development," Hepatology Research, vol. 40, no. 11, pp. 1128-1141, 2010.
 I. V. Martin, E. Borkham-Kamphorst, S. Zok et al., "Platelet-derived growth factor (PDGF)-C neutralization reveals differential roles of PDGF receptors in liver and kidney fibrosis," The American Journal of Pathology, vol. 182, no. 1, pp. 107-117, 2013.
 C. H. Liao, H. Akazawa, M. Tamagawa et al., "Cardiac mast cells cause atrial fibrillation through PDGF-A--mediated fibrosis in pressure-overloaded mouse hearts," Journal of Clinical Investigation, vol. 120, no. 1, pp. 242-253, 2010.
 B. S. Oemar and T. F. Luscher, "Connective tissue growth factor. Friend or foe?" Arterioscler Thrombosis Vascular Biology, vol. 17, no. 8, pp. 1483-1489, 1997.
 D. R. Brigstock, "The connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (CCN) family," Endocrine Reviews, vol. 20, no. 2, pp. 189-206, 1999.
 A. K. Ghosh, "Factors involved in the regulation of type I collagen gene expression: implication in fibrosis," Experimental Biology and Medicine, vol. 227, no. 5, pp. 301-314, 2002.
 M. I. Uzel, A. Kantarci, H. H. Hong et al., "Connective tissue growth factor in drug-induced gingival overgrowth," Journal of Periodontology, vol. 72, no. 7, pp. 921-931, 2001.
 A. Kantarci, S. A. Black, C. E. Xydas et al., "Epithelial and connective tissue cell CTGF/CCN2 expression in gingival fibrosis," Journal of Pathology, vol. 210, no. 1, pp. 59-66, 2006.
 I. E. Blom, R. Goldschmeding, and A. Leask, "Gene regulation of connective tissue growth factor: new targets for antifibrotic therapy?" Matrix Biology, vol. 21, no. 6, pp. 473-482, 2002.
 L. Kennedy, S. Liu, X. Shi-Wen et al., "CCN2 is essential for fibroblast function," Experimental Cell Research, vol. 313, no. 5, pp. 952-964, 2007.
 N. Hayata, Y. Fujio, Y. Yamamoto et al., "Connective tissue growth factor induces cardiac hypertrophy through Akt signaling," Biochemical and Biophysical Research Communications, vol. 370, no. 2, pp. 274-278, 2008.
 D. R. Brigstock, "Strategies for blockingthe fibrogenic actions of connective tissue growth factor (CCN2): from pharmacological inhibition in vitro to targeted siRNA therapy in vivo," Journal of Cell Communication and Signaling, vol. 3, no. 1, pp. 5-18, 2009.
 M. Ponticos, A. M. Holmes, X. Shi-wen et al., "Pivotal role of connective tissue growth factor in lung fibrosis: MAPK-dependent transcriptional activation of type I collagen," Arthritis and Rheumatism, vol. 60, no. 7, pp. 2142-2155, 2009.
 Y. Takeda, "Phagocytosis of mast cell granules by fibroblasts of the human gingiva," Virchows Archiv, vol. 406, no. 2, pp. 197-201, 1985.
 P. K. Nurmenniemi, H. E. Pernu, and M. L. E. Knuuttila, "Mast cell subpopulations in gingival overgrowth induced by immunosuppressive and nifedipine medication," Journal of Periodontology, vol. 75, no. 7, pp. 933-938, 2004.
 S. J. Ruoss, T. Hartmann, and G. H. Caughey, "Mast cell tryptase is a mitogen for cultured fibroblasts," Journal of Clinical Investigation, vol. 88, no. 2, pp. 493-499, 1991.
 J. A. Cairns and A. F. Walls, "Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblasts," Journal of Clinical Investigation, vol. 99, no. 6, pp. 1313-1321, 1997
 M. Artuc, U. Muscha Steckelings, and B. M. Henz, "Mast cell-fibroblast interactions: human mast cells as source and inducers of fibroblast and epithelial growth factors," Journal of Investigative Dermatology, vol. 118, no. 3, pp. 391-395, 2002.
 S. S. Craig and L. B. Schwartz, "Tryptase and chymase, markers of distinct types of human mast cells," Immunologic Research, vol. 8, no. 2, pp. 130-148, 1989.
 K. A. Lindstedt, Y. Wang, N. Shiota et al., "Activation of paracrine TGF-[beta]1 signaling upon stimulation and degranulation of rat serosal mast cells: a novel function for chymase," The Journal of the Federation of American Societies for Experimental Biology, vol. 15, no. 8, pp. 1377-1388, 2001.
 N. Ohuchi, K. Koike, M. Sano et al., "Proliferative effects of angiotensin II and endothelin-1 on guinea pig gingival fibroblast cells in culture," Comparative Biochemistry and Physiology C, vol. 132, no. 4, pp. 451-460, 2002.
 S. Tamilselvan, S. N. Raju, D. Loganathan, S. Kamatchiammal, G. Abraham, and R. Suresh, "Endothelin-1 and its receptors ET(A) and ET(B) in drug-induced gingival overgrowth," Journal of Periodontology, vol. 78, no. 2, pp. 290-295, 2007
 S. He, M. D. A. Gaca, A. R. Mceuen, and A. F. Walls, "Inhibitors of chymase as mast cell-stabilizing agents: contribution of chymase in the activation of human mast cells," Journal of Pharmacology and Experimental Therapeutics, vol. 291, no. 2, pp. 517-523, 1999.
 T. Matsumoto, A. Wada, T. Tsutamoto, M. Ohnishi, T. Isono, and M. Kinoshita, "Chymase inhibition prevents cardiac fibrosis and improves diastolic dysfunction in the progression of heart failure," Circulation, vol. 107, no. 20, pp. 2555-2558, 2003.
 S. Takai, D. Jin, M. Muramatsu, Y. Okamoto, and M. Miyazaki, "Therapeutic applications of chymase inhibitors in cardiovascular diseases and fibrosis," European Journal of Pharmacology, vol. 501, no. 1-3, pp. 1-8, 2004.
 S. He, M. D. A. Gaca, and A. F. Walls, "A role for tryptase in the activation of human mast cells: modulation of histamine release by tryptase and inhibitors of tryptase," The Journal of Pharmacology and Experimental Therapeutics, vol. 286, no. 1, pp. 289-297, 1998.
 T. Subramani, K. Senthilkumar, S. Periasamy, and S. Rao, "Expression of angiotensin II and its receptors in cyclosporine-induced gingival overgrowth," Journal of Periodontal Research, 2012.
 S. E. Campbell and L. C. Katwa, "Angiotensin II stimulated expression of transforming growth factor-[beta]1 in cardiac fibroblasts and myofibroblasts," Journal of Molecular and Cellular Cardiology, vol. 29, no. 7, pp. 1947-1958, 1997
 J. E. J. Schultz, S. A. Witt, B. J. Glascock et al., "TGF-[beta]1 mediates the hypertrophic cardiomyocyte growth induced by angiotensin II," Journal of Clinical Investigation, vol. 109, no. 6, pp. 787-796, 2002.
 F. Yang, A. C. K. Chung, X. Ru Huang, and H. Y. Lan, "Angiotensin II induces connective tissue growth factor and collagen i expression via transforming growth factory-[beta]-dependent and -independent Smad pathways: the role of Smad3," Hypertension, vol. 54, no. 4, pp. 877-884, 2009.
 X. Gao, X. He, B. Luo, L. Peng, J. Lin, and Z. Zuo, "Angiotensin II increases collagen I expression via transforming growth factor-beta1 and extracellular signal-regulated kinase in cardiac fibroblasts," European Journal of Pharmacology, vol. 606, no. 1-3, pp. 115-120, 2009.
 M. M. Martin, J. A. Buckenberger, J. Jiang et al., "TGF-[beta]1 stimulates human at1 receptor expression in lung fibroblasts by cross talk between the Smad, p38 MAPK, JNK, and PI3K signaling pathways," American Journal of Physiology, vol. 293, no. 3, pp. L790-L799, 2007
 K. F. Hilgers and J. F. E. Mann, "ACE inhibitors versus AT1 receptor antagonists in patients with chronic renal disease," Journal of the American Society of Nephrology, vol. 13, no. 4, pp. 1100-1108, 2002.
 A. Schmidt, U. Gruber, G. Bohmig, E. Koller, and G. Mayer, "The effect of ACE inhibitor and angiotensin II receptor antagonist therapy on serum uric acid levels and potassium homeostasis in hypertensive renal transplant recipients treated with CsA," Nephrology Dialysis Transplantation, vol. 16, no. 5, pp. 1034-1037, 2001.
 L. A. Calo, P. A. Davis, B. Giacon et al., "Oxidative stress in kidney transplant patients with calcineurin inhibitor-induced hypertension: effect of ramipril," Journal of Cardiovascular Pharmacology, vol. 40, no. 4, pp. 625-631, 2002.
 M. Clozel and H. Salloukh, "Role of endothelin in fibrosis and anti-fibrotic potential of bosentan," Annals of Medicine, vol. 37, no. 1, pp. 2-12, 2005.
 P. Teder and P. W. Noble, "A cytokine reborn? Endothelin-1 in pulmonary inflammation and fibrosis," American Journal of Respiratory Cell and Molecular Biology, vol. 23, no. 1, pp. 7-10, 2000.
 A. Ishihata and Y. Katano, "Role of angiotensin II and endothelin-1 receptors in aging-related functional changes in rat cardiovascular system," Annals of the New York Academy of Sciences, vol. 1067, no. 1, pp. 173-181, 2006.
 W. Neuhofer and D. Pittrow, "Role of endothelin and endothelin receptor antagonists in renal disease," European Journal of Clinical Investigation, vol. 36, no. 3, pp. 78-88, 2006.
 K. Ahmadi-Simab, B. Hellmich, and W. L. Gross, "Bosentan for severe pulmonary arterial hypertension related to systemic sclerosis with interstitial lung disease," European Journal of Clinical Investigation, vol. 36, no. 3, pp. 44-48, 2006.
 T. Subramani, L. Dhanaraj, K. Senthilkumar, S. Periasamy, G. Abraham, and S. Rao, "Expression of TNF- [alpha]and RANTES in drug-induced human gingival overgrowth," Indian Journal of Pharmacology, vol. 42, no. 3, pp. 174-177, 2010.
 T. Masaki, S. Miwa, T. Sawamura, H. Ninomiya, and Y. Okamoto, "Subcellular mechanisms of endothelin action in vascular system," European Journal of Pharmacology, vol. 375, no. 1-3, pp. 133-138, 1999.
 A. Ergul, D. Jupin, M. H. Johnson, and L. M. Prisant, "Elevated endothelin-1 levels are associated with decreased arterial elasticity in hypertensive patients," Journal of Clinical Hypertension, vol. 8, no. 8, pp. 549-554, 2006.
 A. Horstmeyer, C. Licht, G. Scherr, B. Eckes, and T. Krieg, "Signalling and regulation of collagen I synthesis by ET-1 and TGF-[beta]1," FEBS Journal, vol. 272, no. 24, pp. 6297-6309, 2005.
 Z. A. Khan, H. Farhangkhoee, J. L. Mahon et al., "Endothelins: regulators of extracellular matrix protein production in diabetes," Experimental Biology and Medicine, vol. 231, no. 6, pp. 1022-1029, 2006.
 X. Shi-wen, L. Kennedy, E. A. Renzoni et al., "Endothelin is a downstream mediator of profibrotic responses to transforming growth factor [beta] in human lung fibroblasts," Arthritis and Rheumatism, vol. 56, no. 12, pp. 4189-4194, 2007
 P. Shephard, B. Hinz, S. Smola-Hess, J. J. Meister, T. Krieg, and H. Smola, "Dissecting the roles of endothelin, TGF-[beta] and GM-CSF on myofibroblast differentiation by keratinocytes," Thrombosis and Haemostasis, vol. 92, no. 2, pp. 262-274, 2004.
 T. H. Cheng, P Y. Cheng, N. L. Shih, I. B. Chen, D. L. Wang, and J. J. Chen, "Involvement of reactive oxygen species in angiotensin II-induced endothelin-1 gene expression in rat cardiac fibroblasts," Journal of the American College of Cardiology, vol. 42, no. 10, pp. 1845-1854, 2003.
 H. A. M. Mucke, "Pulmonary arterial hypertension: on the way to a manageable disease," Current Opinion in Investigational Drugs, vol. 9, no. 9, pp. 957-962, 2008.
 S. G. Raja, "Macitentan, a tissue-targeting endothelin receptor antagonist for the potential oral treatment of pulmonary arterial hypertension and idiopathic pulmonary fibrosis," Current Opinion in Investigational Drugs, vol. 11, no. 9, pp. 1066-1073, 2010.
 M. C. Kowala, N. Murugesan, J. Tellew et al., "Novel dual action AT1 and ETA receptor antagonists reduce blood pressure in experimental hypertension," Journal of Pharmacology and Experimental Therapeutics, vol. 309, no. 1, pp. 275-284, 2004.
 C. Palaniswamy, D. R. Selvaraj, and D. Palaniappan, "Dual angiotensin II and endothelin receptor antagonists," American Journal of Therapeutics, vol. 18, no. 3, pp. e67-e70, 2011.
Tamilselvan Subramani, (1) Vidhya Rathnavelu, (2) and Noorjahan Banu Alitheen (1)
(1) Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
(2) Department of Oral and Maxillofacial Pathology, Faculty of Dental Science, Sri Ramachandra University, Porur, Chennai 600116, India
Correspondence should be addressed to Noorjahan Banu Alitheen; firstname.lastname@example.org
Received 4 March 2013; Accepted 29 March 2013
Academic Editor: Giamila Fantuzzi
|Printer friendly Cite/link Email Feedback|
|Author:||Subramani, Tamilselvan; Rathnavelu, Vidhya; Alitheen, Noorjahan Banu|
|Publication:||Mediators of Inflammation|
|Date:||Jan 1, 2013|
|Previous Article:||Green tea extract supplementation induces the lipolytic pathway, attenuates obesity, and reduces low-grade inflammation in mice fed a high-fat diet.|
|Next Article:||NAG-1/GDF15 transgenic mouse has less white adipose tissue and a reduced inflammatory response.|