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The actions of methoprene acid on a developing urodele.

The objective of this study was to characterize the biological actions of methoprene acid (MA), a metabolite of the insecticide methoprene, on a developing axolotl, Ambystoma mexicanum, and to assess levels of developmental stability following treatment of embryos and hatchlings. We tested the null hypotheses that (1) MA would produce no developmental malformations similar to those produced by the positive control, retinoic acid (RA) and (2) the degree of vulnerability to treatment would not differ between embryos and hatchlings. Animals were exposed to 1 of 4 concentrations of MA (0, 30, 150, and 300 ug/L), or RA (0, 0.3, 1.5, 3.0 ug/L), depending on the respective treatment group. Additionally, half the subjects were treated from blastula stage to hatching only, while the second half were treated beginning at hatching for the duration of the experiment. The experiment was terminated when all animals reached full hind limb development. Developmental stability was determined by measuring fluctuating asymmetry of the hind limbs, forelimbs, and eyes. Craniofacial and limb malformations that were previously characterized as retinoid-like were seen in both MA- and RA- treated animals. Effects on developmental stability were consistently observed as fluctuating asymmetry of the forelimbs and hind limbs in MA- and RA- treated animals.

Elena M. Lopez and David O. Norris *. Department of EPO Biology, University of Colorado, Boulder.

* Denotes membership in the Colorado-Wyoming Academy of Science.
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Author:Lopez, Elena M.; Norris, David O.
Publication:Journal of the Colorado-Wyoming Academy of Science
Article Type:Brief Article
Geographic Code:1USA
Date:Apr 1, 2002
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