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The STR polymorphism [(AAAAT).sub.n] within the intron 1 of the tumor protein 53 (TP53) locus in 17 populations of different ethnic groups of Africa, America, Asia and Europe.

RESUMEN

Se estudio el polimorfismo del microsatelite [(AAAAT).sub.n] del intron 1 del gene TP53 en 17 poblaciones de 3 grupos etnicos: europeos, asiaticos, y africanos subsaharianos, asi como de la poblacion hibrida de Costa Rica (en total 1968 muestras). Tres alelos, 126/7 (pares de bases/copias de la repeticion), 131/8 y 136/9 fueron los mas frecuentes en todas las poblaciones, aunque se observaron otros alelos usualmente a frecuencias menores al 10%. Las heterocigosis observadas variaron de 0.351 a 0.829. La distribucion de la diversidad parece concordar con el origen geografico de las muestras y con la historia de las poblaciones estudiadas. Una prueba estadistica indica que el evento mutacional que mas alelos nuevos produce en este marcador es el de un solo paso (expansion o contraccion de una sola copia de la repeticion). El indice de fijacion RST mostro los efectos de la subdivision de poblaciones solo dentro del grupo de los asiaticos y mostro falta de sensibilidad cuando los grupos comparados eran de niveles superiores de clasificacion (europeos, asiaticos, y africanos) o cuando la comparacion se hizo entre los grupos mas antiguos (africanos y europeos).

Palabras clave: genetica de poblaciones, microsatelites, polimorfismos, europeos, asiaticos, africanos del sub-Sahara.

Abstract: The STR [(AAAAT).sub.n] within intron 1 of the TP53 locus was screened in 17 populations from 3 main ethnic groups: Europeans, Asiatics, and Africans, and from the hybrid population of Costa Rica (1968 samples). Three alleles, 126/7 (bp/copies of the repeat), 131/8 and 136/9 were the most prevalent in all populations. Other alleles rarely reached frequencies of 10% or higher. Observed heterozygosities ranged between 0.351 and 0.829. Patterns of diversity fit well with both the geographic origin of the samples and the history of the populations screened. A statistical test suggests that single-step mutational events have been the main mechanism producing new alleles at this locus. Fixation indexes ([R.sub.ST]) for this marker showed an effect of population subdivision on divergence only within the Asiatic group; they were insensitive at the level of major ethnic groups as well as within Africans and within Europeans.

Key words: population genetics, microsatellite polymorphism, Europeans, Asiatics, sub-Saharan Africans.

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The tumor protein 53 locus (TP53) (MIM No. 191170, GDB ID 120445) maps to 17p13.1 (Isobe et al. 1986). The gene spans 12.5 kb, is segmented in 11 exons, the first of which is not translated, and codes for a 43.5 kDa protein (p53) involved in cellular responses to genetic damage as mediator in DNA repair, in growth arrest at the G 1-S checkpoint of the cell cycle, or as an inductor of apoptosis (Hesketh 1997, Janus et al. 1999). Although several polymorphic regions have been described within the locus, population genetic studies are not abundant. Exceptional are the works of two groups which have studied the haplotypic variation defined by the presence/absence of a 16bp duplication within intron 3, of a BstU1 restriction site within exon 4 and of an MspI restriction site within intron 6 in Northern Europeans, Chinese and Africans (Sjalander et al. 1995, 1996), and in Amerindians, Blacks and European descendants from Brazil (Gaspar et al. 2001, 2002). On the other hand, several mutations have been directly implicated in approximately 50% of all human cancers (Janus et al. 1999) -for an extensive review see OMIM. In this association, Ridanpaa et al. (1995) have studied samples of archival lung cancer tissue using a pentanucleotide [(AAAAT).sub.n] within the 6.1 kb-long intron 1 of the locus, a polymorphism first reported by Futreal et al. (1991) and further characterized by Hahn et al. (1995). The methodological improvements developed by Hahn et al. (1993) facilitated the screening of large samples. Hence, population data of this polymorphism have been reported for two samples, one from Germany (Hahn et al. 1995) and the other from Northern Portugal (Gusmao et al. 1997).

In this study, new population data of this STR are reported for 17 populations of 3 major ethnic groups, Europeans, Asiatics and Africans. The data permitted documentation of some of the effects caused by the human spread through the world on the diversity patterns of this STR, and also to statistically explore the main mutational mechanism from which new alleles originate and to test the potential of this marker in phylogenetic studies.

MATERIALS AND METHODS

Data were independently obtained by two groups, one from the Section of Human Genetics, Institute for Health Research (INISA), University of Costa Rica, Costa Rica, and the other from the Institute of Biochemistry, Justus-Liebig University at Giessen, Germany. We decided to pool our results to achieve a wider perspective on the distribution and evolution of the polymorphism; therefore, some methodological differences in laboratory methods are to be noted.

Populations

Samples analyzed in Costa Rica are from 10 Amerindian populations, 1 group of Afro-Costa Ricans and 1 sample of the general Costa Rican population. Groups screened in Germany are 2 from Africa, 1 from Germany, 1 from Turks living or born in Germany and 1 from The Philippines. The populations were grouped into 3 major ethnic categories: (1) Asiatics, the Amerindians and the Filipinos; (2) sub-Saharan Africans (in this paper refered as Africans), the Namibian, Ugandan and the Afro-Costa Ricans; (3) Europeans, the Germans and the Turks. Data for the same marker from Northern Portugal reported by Gusmao et al. (1997) were added to the European group. The general Costa Rican population was considered as hybrid because of its multiethnic origin (Morera et al. 2001, 2003).

1. Asiatics

1.a. Amerindians: A total of 576 individuals belonging to six Chibchan-speaking tribal groups of Lower Central America (38 Bribri from Mojoncito, 129 Cabecar from Alto Telire, 58 Cabecar from Ujarras, 34 Guatuso from Tonjibe/Margarita, 74 Guaymi from Abrojo, 33 Guaymi from Dos Brazos, 34 Huetar from Quitirrisi, 100 Huetar from Zapaton, all in Costa Rica, and 76 Teribe from Sieyik, in Panama) were sampled between 1986 and 1998. The Chibchan Stock comprises 21 Central and South American aboriginal languages distributed along the Atlantic coasts of Honduras and Nicaragua, most of Costa Rica, Panama, almost the whole Colombian territory and the North of Venezuela (Constenla-Umana 1991). An additional sample of 75 Amerindians was obtained in Matambu, Costa Rica, from a group whose ancestors belonged to an area of Mesoamerican (Central Amerind) cultural tradition and their now extinct language belonged to the Oto-Manguean Family (e.g. Newson 1987). Sampling methods were as described elsewhere (Barrantes et al. 1990).

1.b. Filipinos: 84 Filipino samples were obtained at the Panay Island, The Philippines, between 1992 and 1995. All are unrelated donors, collaborating as controls in a study on X-linked dystonia parkinsonism. Samples were cordially provided by Prof. Ulrich Muller, Institute of Human Genetics, Justus-Liebig University, Giessen.

2. Africans

2.a. Namibian: 99 samples from an Ovambo group, a Bantu tribe, were obtained in Northern Namibia, from voluntary blood donors to the Namibian Red Cross in 1994. Prof. Carsten Wolff, Halberstadt, kindly made them available to us.

2.b. Ugandan: 81 samples from a mixed population of Bantu people (Toro, Kiga and Tutsi), were obtained during a study on onchocercosis at the Kigoyera parish, Kabarole district, Western Uganda in October 1991. They were kindly contributed by Prof. Dietrich W. Buttner, Bernhard-Nocht Institute for Tropical Medicine, Hamburg.

2.c. Afro-Costa Rican: 193 samples of black donors were obtained at the seaport city Limon between 1999 and 2000. The origins of this group in Costa Rica dates back to 1872 when they were brought from Jamaica to labor in the construction of the railroad linking Limon with San Jose, the capital city of the country (Melendez and Duncan 1979). Their exact African origins cannot be determined, nonetheless, it is certainly true that they are a mixture of peoples from different places (Melendez and Duncan 1979, Lobo-Wiehoff and Melendez-Obando 1997).

3. Europeans

3.a. Germans: The total sample adds up to 263 individuals: Bad Oeynhausen, North Rhine-Westphalia, 147 blood donors collected in 1998 and kindly provided by Prof. Carsten Wolff, Halberstadt; Hanover, 20 patients (bladder cancer) of the School of Medicine, sampled between 1995 and 1998, and, 96 donors from the whole area of Hanover, Lower Saxony, sampled between 1990 and 1992.

3.b. Turks: The sample, 88 individuals, was obtained in the Rhine-Main region, Germany, between 1994 and 1998. It comprises non-related patients afflicted with cystic fibrosis or persons suspected to be at risk of the disease. They were either immigrants from different regions of Turkey or individuals born to Turkish parents in Germany. The samples were kindly provided by Daniela Steinberger, Institute of Human Genetics, Justus-Liebig University, Giessen.

3.c. Portuguese: Data from Northern Portugal were taken from a published work (Gusmao et al. 1997) and added to the Europeans for the statistical analyses.

4. Hybrid

4.a. Costa Rican: 407 samples were obtained in 7 different regions of the country between 1996 and 1998.

Molecular screening

DNA extraction

DNAs analyzed in Costa Rica were extracted from whole peripheral blood (ACD) either with phenol-chloroform (Hermann and Frischauf 1987) or with NaCl (Miller et al. 1988). DNAs screened in Germany were also extracted from whole blood (EDTA) with phenol-chloroform.

PCR conditions

a. Conditions employed in Costa Rica. The oligonucleotide primers used were those described by Hahn et al. (1993). PCR-conditions were those of Ridanpaa et al. (1995) but scaling down reaction volumes to 25[micron]l instead of 50 [micron]l.

b. Conditions employed in Germany: Two combinations of primers were used after Hahn et al. (1993, 2001). PCR conditions were according to Hahn et al. (2001).

Separation of PCR products

a. Conditions employed in Costa Rica. Native PAGE (3% stacking, 2cm below the comb level, and 10% resolving). Gels were stained with silver nitrate.

b. Conditions employed in Germany. Native and denaturing PAGE, and, denaturing capillary electrophoreses (Hahn et al. 2001).

Statistical analyses

Allele frequencies were estimated by gene counting and their standard deviations were calculated assuming a multinomial distribution; gene diversities were calculated after Nei (1987). Goodness of fit to the Hardy-Weinberg expectations, evaluated by a modified Fisher's exact test (Guo and Thompson 1992), and fixation indexes, [R.sub.IS], [R.sub.ST] and [R.sub.IT], and genetic distances were estimated according to Slatkin (1995) using the ARLEQUIN program (Schneider et al. 1997). A multidimensional analysis (Borg 1997) was performed using the STATISTICA for Windows, release 4.5 Package (StatSoft Inc. 1993).

The mutational origin of the allele-frequency distributions observed was assumed to follow a step-wise mutation model (Ohta and Kimura 1973, Moran 1975, Wehrhan 1975). The null hypothesis, that mutation occurs at one step events, was tested using the Markov-chains recursive method (Griffiths and Tavare 1994, Nielsen 1997) which takes the form of a likelihood ratio; i.e. the probability of a one-step model relative to that of a multi-step model (-2log[L([theta],q=0)/L([theta],q)]), where [theta] = 4Ne[my], Ne is the effective population size, [my] the mutation rate (Ohta and Kimura 1973) and q the proportion of multi-step mutations (0 at one-step). Nielsen (1997) showed that if q = 0 the likelihood ratio resembles a [ji al cuadrado] distribution when sample sizes are large enough. [theta] was estimated by maximum likelihood and both components of the likelihood ratio were calculated by series of one million iterations using the Microsatellite Analysis (MISAT) Program (Nielsen 1997).

RESULTS

The absolute and relative phenotype frequencies obtained in the Asiatic group are presented in Table 1. Those found in the Africans, Europeans and the Hybrid population of Costa Rica are shown in Table 2. Their corresponding allele frequencies, observed and expected (gene diversities) heterozygosities and their Hardy-Weinberg fit are summarized in Tables 3 and 4.

Although three alleles (126, 131, 136 bp) are the most frequent in all populations, the number of phenotypes and gene diversities follow patterns concordant with the geographic origin of each population as if they had spread from an African centroid. Africans, the oldest of them under this perspective, have, as would be expected, more alleles (7-9) more phenotypes (18-19) and higher gene diversities (0.739-0.784) than the other groups. Europeans, have 4-7 alleles, 6-14 phenotypes and gene diversities ranging from 0.55-0.705. Amerindians have 3-6 alleles, the number of phenotypes ranged between 3 and 12 and gene diversities between 0.377 and 0.729. However, setting apart the Guatuso, Huetar and Chorotega, tribes with the highest degree of interethnic admixture (Barrantes 1993), the rest of the tribes show an even lower diversity: 3 alleles, the number of phenotypes is 4 or 5 and the gene diversities range between 0.351 and 0.697. Filipinos have 9 alleles, 16 phenotypes and a gene diversity of 0.704, values intermediate between Africans and Europeans. The hybrid population of Costa Rica reflects, through its high diversity, 7 alleles, 20 phenotypes and a gene diversity of 0.705, its multiethnic origin.

Genetic distances (Table 5) and a multidimensional analysis (Fig. 1) were performed to obtain a complementary perspective of the path followed by this STR during the divergence of these populations and to test the confidence of the data obtained from the samples analyzed (however, these statistics were not intended to estimate the actual genetic distances among the populations compared, i.e. they must be regarded in their qualitative sense). Populations clustered in three groups, an array in agreement with expectations according to their geographic origin and history: the Europeans, the Amerindians and a third one including the Africans, the Filipino and the hybrid population of Costa Rica.

The fixation indexes for the major groups, Europeans, Asiatics, Africans and hybrid were [R.sub.IS] = 0.0112 (p < 0.001), [R.sub.IT] = 0.0197 (p < 0.001) and [R.sub.ST] = 0.0085 (p > 0.05). Thus variation is significant among individuals within subpopulations ([R.sub.IS]) as well as among individuals within the total of all populations ([R.sub.IT]). In contrast, [R.sub.ST] did not disclose any significant effect of population subdivision on the divergence of this STR at this level of population subdivision. The same was the case within Africans ([R.sub.ST] = 0.0015, p > 0.05) and within Europeans ([R.sub.ST] = -0.0005, p > 0.05). However, within the Asiatics [R.sub.ST] showed that subdivision in the three possible combinations has been significant: within the Chibchan ([R.sub.ST] = 0.1411, p < 0.001), Chibchan total vs. Mesoamerican ([R.sub.ST] = 0.0199, p < 0.05), and Chibchan total vs. Mesoamerican vs. Filipino ([R.sub.ST] = 0.0324, p< 0.001).

The data obtained also permitted a statistical exploration of the possible molecular mechanisms originating new alleles at this STR. Expansion or contraction in the number of tandem repeats could occur by single- or by multi-step events, i.e. the original allele could win or lose a single repeat or multiple repeats per mutational event. Table 6 summarizes likelihood ratios for a one-step mutation model relative to a multi-step mutation model in ten populations (the Chibchan tribes were pooled asa single group). All ratios indicate that the single-step model better explains the observed allele-frequency distributions. Therefore, as [theta] measures the joint effect of mutation, through its rate, and population structure, through Ne, ir would be safe to postulate that mutation on this marker acts mainly by single steps.

DISCUSSION

The characterization of genetic variation at an STR, the [(AAAAT).sub.n] within intron 1 of the TP53 locus in 17 populations of 3 major ethnic groups, permitted the documentation of some of the effects of the spread of the human species through the world on its patterns of diversity. The subdivisions of ancestral populations, mainly by fissions, were events often associated with bottlenecks. Since the derived groups were small groups of related individuals, one of the main consequences during differentiation was the loss of alleles by genetic drifl (Fix 1999). In the groups screened here the reduction of diversity follows an almost linear fashion from the African Continent: Africans, the oldest and most diverse, Europeans and Filipinos intermediate between the former and the youngest and less diverse Amerindians. Assuming a dispersal of the modern humans from an African centroid, this is an indication of the relative good representation of the samples screened and the discriminative power of the marker. The multidimensional analysis performed supports this interpretation, which is in very good agreement with worldwide studies based on many more genetic markers (e.g. Cavalli-Sforza et al. 1994). However, it must be kept in mind that it is based on a single marker, and, second, as noted by Harpending and Rogers (2000), measures of genetic distances of populations covering large geographic areas are often misleading.

Among the fixation indexes, [R.sub.ST], like genetic distances, also showed to be insensitive at major scales. A possible cause for this is the fact that three alleles (136/9, 131/8 and 126/7) are the most common in all populations and even though the size of the alleles ranged between 111/4 and 151/12, alleles other than the commonest rarely reached 10% or a higher frequency, which in turn could be the result of random fixation of alleles that can act differently on different loci. Some of these loci would have fixed different alleles in different parts of the world, as has been documented (Zerylnick et al. 1995, Deka et al. 1996, 1999). It could also be the case that they preserve the same alleles in all populations as has been shown for the CSF1R locus (Deka et al. 1999). Balancing selection cannot be ruled out given the important role of TP53 in genomic stability (Janus et al. 1999), and although the marker screened is located at an intron, increased instability of mRNA from alleles with motif-repeat numbers outside the range observed could affect the normal function of the gene. In addition, some alleles might have been indirectly selected by hitchhiking if they were in cis with alleles conferring increased fitness. The latter hypothesis could be tested extending the screening to haplotypic variation.

The Filipino and the hybrid Costa Rican population deserve comments aside. The Filipino shows an intermediate diversity between the Africans and Europeans and was unexpectedly placed at the multidimensional analysis closer to the Africans rather than to the Asiatics. Cavalli-Sforza et al. (1994) found a high diversity in this group using classical markers (blood groups and blood proteins) and explained the fact as a sign of a putative multiple origin (Negrito, Malayan and Chinese). In addition, the populations of Southeast Asia stem from an early split from the group that led to the other Asiatic groups (Cavalli-Sforza et al. 1994). Both facts, fusion of different ethnic groups each originating from a time when the ancestral (African) gene diversity was not diminished by bottlenecks could explain their higher diversity and closer proximity to the Africans than to the Amerindians. Regarding the high diversity value shown by the hybrid population of Costa Rica, it also reflects its multiethnic origin. Morera et al. (2003) estimated that the Costa Rican population has 61% European -mainly Spanish-, 30% Amerindian and 9% Africans genetic ancestry. Its nearer position to the African cluster is in accordance with that estimate. In the end, admixture can be seen as a regression to the ancestral conditions.

The data obtained also permitted a statistical analysis of the main molecular mechanism producing new alleles at this marker. The test favors a model of expansion or contraction in the number of tandem repeats by single-steps, although this does not rule out multi-step mutations. This conclusion is concordant with empirical data of Brinkmann et al. (1998) who analyzed 10 844 parent/child allelic transfers at 23 STRs and found that 22 out of 23 mismatches detected were single-step and only 1 a two-step.

AKNOWLEDGMENTS

We thank Omar Achi for his fine laboratory work. We also thank Carsten Wolff (Halberstadt, Germany), Ulrich Muller, Daniela Steinberger (Giessen, Germany) and Dietrich Buttner (Hamburg, Germany) for providing us the samples from Uganda, Namibia, The Philippines, Germany and Turkey. Support of Alfred Pingoud (Giessen) and Peter Lichter (Heidelberg), is also greatly acknowledged. This research was partially funded by the Universidad de Costa Rica (Grants 742-93-903, 111-97-522 and 742-A1-502) and by the Consejo Nacional de Investigaciones Cientificas y Tecnologicas de Costa Rica (CONICIT).

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Deka, R, P.P. Majumder, M.D. Shriver, D.N. Stivers, Y. Zhong, L.M. Yu, R. Barrantes, S.J. Yin, T. Miki, J. Hundrieser, C.H. Bunker, S.T. McGarvey, S. Sakallah, R.E. Ferrell & R. Chakraborty. 1996. Distribution and evolution of CTG repeats at the myotonin protein kinase gene in human populations. Genome Res. 6: 142-154.

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Fix, A.G. 1999. Migration and Colonization in Human Microevolution. Cambridge University, Cambridge.

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Gaspar, P.A., M.H. Hutz, F.M. Salzano, K. Hill, A.M. Hurtado, ML. Petz-Erler, L.T. Tsuneto & T.A. Weimer. 2002. Polymorphisms of CYP1A1, CYP2E1, GSTMI, GSTT1, and TP53 genes in Amerindians. Am. J. Phys. Anthrop. 119: 249-256.

Griffiths, R.C. & S. Tavare. 1994. Simulating probability distributions. Theor. Pop. Biol. 46: 131-159.

Guo, S. & E. Thompson. 1992. Performing the exact test of the Hardy-Weinberg proportion for multiple alleles. Biometrics 48: 361-372.

Gusmao, L., M. J. Prata, A. Amorim, F. Silva & I. Bessa. 1997. Characterization of four short tandem repeat loci (THO1, VWA31/A, CD4, and TP53) in Northern Portugal. Hum. Biol. 69: 31-40.

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INTERNET REFERENCES

GDB (TM). 2003. Human Genome Database [database online]. Available from Internet: URL http://www.gdb.org (Downloaded: 11 Aug. 2003; GDB node: The Netherlands: http://gdb.cmbi.kun.nl/gdb/) GDB Data Type: Gene; object name: TP53; Accession ID: GDB: 120445.

OMIM (TM). 2000. Online Mendelian Inheritance in Man. Johns Hopkins University, Baltimore, MD, MIM 191170. Date of last modification 7/24/2003. (Downloaded: http://www.ncbi.nlm.nih.gov/latbinpost/Omirrddispmim? 191170#.).

Jorge Azofeifa (1) *, Meinhard Hahn (2), Edward Ruiz (1), Lars Hummerich (2), Ana I. Morales (3), Gerardo Jimenez (4) & Ramiro Barrantes (1)

(1) Escuela de Biologia and Instituto de Investigaciones en Salud (INISA), Universidad de Costa Rica

(2) Institut fur Biochemie, Justus-Liebig-Universitat Giessen, Germany (Present address: Deutsches Kxebsforschungszentrum, Abteilung Molekulare Genetik (H0700), Heidelberg, Germany)

(3) Organismo de Investigaciones Judiciales, Costa Rica

(4) Centro de Investigaciones en Hemoglobinas Anormales y Trastornos Afines (CIHATA), Universidad de Costa Rica

* Correspondence to: Jorge Azofeifa, Instituto de Investigaciones en Salud (INISA), Universidad de Costa Rica, Ciudad Universitaria "Rodrigo Facio", San Jose, Costa Rica, Central America. Tel.: (+506) 224 36 68, Fax: (+506) 207 51 30; azofeifa@biologia.ucr.ac.cr

Received 13-VIII-2003. Corrected 15-X-2003. Accepted 02-XI-2003.
TABLE 1

Phenotype frequencies of the STR [(AAAAT).sub.n] within intron 1 of the
TP53 locus in Mongoloids

                                                           Guatuso
                  Bribri        Cabecar       Cabecar     Tonjibe/
Phenotype (1)    Mojoncito    Alto Telire     Ujarras     Margarita

141/136                                                   3(0.088)
141/131
141/126
141/121
141/116
136               5(0.132)      4(0.031)      5(0.086)
136/131                                       5(0.086)
136/126          15(0.395)     45(0.349)     20(0.345)    19(0.559)
136/121
136/116
131
131/126           1(0.026)     13(0.101)     13(0.224)     2(0.059)
131/121
131/116
126              17(0.447)     67(0.519)     15(0.259)    10(0.294)
126/121
126/116
n                   38            129           58           34

                  Guaymi        Guaymi        Huetar       Huetar
Phenotype (1)     Abrojo      Dos Brazos    Quitirrisi    Zapaton

141/136
141/131
141/126
141/121
141/116
136              45(0.608)     9(0.273)      3(0.088)     27(0.27)
136/131           7(0.095)     3(0.091)      3(0.088)      5(0.05)
136/126          17(0.230)    20(0.606)      9(0.265)     22(0.22)
136/121                                      4(0.118)      1(0.01)
136/116                                                    2(0.02)
131                                          1(0.029)      7(0.07)
131/126           2(0.027)                   5(0.147)     16(0.16)
131/121                                      2(0.059)      1(0.01)
131/116                                                    1(0.01)
126               3(0.040)     1(0.030)      3(0.088)     15(0.15)
126/121                                      4(0.118)      1(0.01)
126/116                                                    2(0.02)
n                   74            33            34          100

                                                         Filipino
                  Teribe        Total       Chorotega      Panay
Phenotype (1)     Sieyik       Chibcha       Matambu      Island

141/136                         3(0.005)                  1(0.012)
141/131                                                   1(0.012)
141/126                                                   8(0.095)
141/121                                                   1(0.012)
141/116                                                   1(0.012)
136               7(0.092)    105(0.182)    16(0.213)     1(0.012)
136/131           3(0.039)     26(0.045)    10(0.133)     5(0.06)
136/126          33(0.434)    200(0.347)    28(0.373)     9(0.107)
136/121                         5(0.009)     3(0.04)
136/116                         2(0.003)                  1(0.012)
131                             8(0.014)                  8(0.095)
131/126           4(0.053)     56(0.097)     9(0.12)     21(0.25)
131/121                         3(0.005)                  2(0.024)
131/116                         1(0.002)                  4(0.048)
126              29(0.382)    160(0.278)     7(0.093)    15(0.179)
126/121                         5(0.009)     2(0.027)     2(0.024)
126/116                         2(0.003)                  4(0.048)
n                   76           576           75           84

(1) Numbers indicate size of the bands in base pairs (bp). The band of
141bp contains 10 (AAAAT) repeats and the 116bp 5 repeats (Hahn et al.
1995).

TABLE 2

Phenotype frequencies of the STR [(AAAAT).sub.n] within intron 1 of the
TP53 locus in Negroid, Hybrid and Caucasoid populations

                               Negroid
                  African      African       Afro-         Hybrid
Phenotype (1)     Namibia      Uganda      Costa Rica    Costa Rica

151/141           1(0.01)
151/136           2(0.02)
151/126                        1(0.012)
146/136                                     2(0.01)       1(0.002)
146/131                        1(0.012)
146/126                        1(0.012)                   3(0.007)
146/116           1(0.01)                   1(0.005)
141                                         1(0.005)      4(0.01)
141/136           4(0.04)                   3(0.016)      8(0.02)
141/131                       10(0.101)     4(0.049)      5(0.026)
141/126                        4(0.04)      3(0.037)      6(0.031)
141/121
141/116                        1(0.01)      2(0.025)
136               2(0.02)      2(0.025)    11(0.057)     25(0.061)
136/131          12(0.121)     9(0.111)    35(0.181)     88(0.216)
136/126           9(0.091)     7(0.086)    23(0.119)     75(0.184)
136/121                        1(0.012)     3(0.016)      3(0.007)
136/116           6(0.061)     1(0.012)     5(0.026)      3(0.007)
131               2(0.02)      8(0.099)    16(0.083)     64(0.157)
131/126          17(0.172)    17(0.21)     40(0.207)     71(0.174)
131/121           1(0.01)      3(0.037)     7(0.036)      5(0.012)
131/116           6(0.061      2(0.025)     4(0.021)      4(0.01)
126              13(0.131)    12(0.148)    21(0.109)     34(0.084)
126/121           4(0.04)                   7(0.036)      7(0.017)
126/116           3(0.03)      4(0.049)     2(0.01)       1(0.002)
126/111                        3(0.037)
121/116           1(0.01)                                 1(0.002)
116                                         1(0.005)
n                   99           81           193           407

                                Caucasoid

                  European       Asian        European
Phenotype (1)     Germany       Turkey      Portugal (2)

151/141
151/136
151/126
146/136
146/131                                       1(0.004)
146/126
146/116
141
141/136           1(0.004)                    2(0.008)
141/131           7(0.017)      3(0.011)      3(0.012)
141/126           2(0.005)      1(0.004)
141/121           1(0.002)
141/116
136              14(0.053)      5(0.057)     18(0.072)
136/131          77(0.293)     25(0.284)     75(0.301)
136/126          14(0.053)     10(0.114)     18(0.072)
136/121           4(0.015)                    10(0.04)
136/116                                       2(0.008)
131              104(0.395)    29(0.33)      63(0.253)
131/126           27(0.103)    14(0.159)     22(0.088)
131/121           11(0.042)     5(0.057)     17(0.068)
131/116                                       3(0.012)
126               5(0.019)                    10(0.04)
126/121           2(0.008)                     5(0.02)
126/116
126/111
121/116
116
n                   263           88            249

(1) Numbers indicate size of the bands in base pairs (bp). The band of
151bp contains 12 (AAAAT) repeats and the 11bbp 5 repeats (Hahn et al.
1995).

(2) Data from Gusmao et al. (1197).

TABLE 3

Allele frequencies, observed and expected heterozygosities, and
Hardy-Weinberg equilibrium test of the STR [(AAAAT).sub.n] within
intron 1 of the TP53 locus in Mongoloid populations: Amerindians of
Costa Rica and Panama and in Filipinos

                      Bribri            Cabecar           Cabecar
Allele (1)          Mojoncito         Alto Telire         Ujarras

141 (10)
136 (9)                    0.329             0.205             0.302
                   [+ o -] 0.054     [+ o -] 0.025     [+ o -] 0.043
131 (8)                    0.013             0.050             0.155
                   [+ o -] 0.013     [+ o -] 0.014     [+ o -] 0.034
126 (7)                    0.658             0.744             0.543
                   [+ o -] 0.055     [+ o -] 0.027     [+ o -] 0.047
121 (6)
116 (5)
Observed                   0.421             0.450             0.655
Heterozygosity
Gene                       0.465             0.403             0.595
Diversity          [+ o -] 0.039     [+ o -] 0.031     [+ o -] 029
H-W P (2)                  0.803             0.144             0.555
                   [+ o -] 0.001     [+ o -] 0.001     [+ o -] 0.002

                   Guatuso Ton-         Guaymi            Guaymi
Allele (1)        jibe/Margarita        Abrojo          Dos Brazos

141 (10)                   0.044
                   [+ o -] 0.025
136 (9)                    0.324             0.0770            0.621
                   [+ o -] 0.057     [+ o -] 0.035     [+ o -] 0.060
131 (8)                    0.029             0.061             0.046
                   [+ o -] 0.021     [+ o -] 0.020     [+ o -] 0.026
126 (7)                    0.603             0.169             0.333
                   [+ o -] 0.060     [+ o -] 0.031     [+ o -] 0.059
121 (6)
116 (5)
Observed                   0.706             0.351             0.697
Heterozygosity
Gene                       0.537             0.377             0.509
Diversity          [+ o -] 0.044     [+ o -] 0.044     [+ o -] 0.042
H-W P (2)                  0.005             0.657             0.054
                   [+ o -] 0.0002    [+ o -] 0.001     [+ o -] 0.001

                      Huetar            Huetar            Teribe
Allele (1)          Quitirrisi          Zapaton           Sieyik

141 (10)

136 (9)                    0.324             0.420             0.329
                   [+ o -] 0.057     [+ o -] 0.035     [+ o -] 0.038
131 (8)                    0.177             0.185             0.046
                   [+ o -] 0.047     [+ o -] 0.028     [+ o -] 0.017
126 (7)                    0.353             0.355             0.625
                   [+ o -] 0.058     [+ o -] 0.034     [+ o -] 0.039
121 (6)                    0.147             0.015
                   [+ o -] 0.043     [+ o -] 0.009
116 (5)                                      0.025
                                     [+ o -] 0.011
Observed                   0.794             0.510             0.526
Heterozygosity
Gene                       0.729             0.666             0.502
Diversity          [+ o -] 0.022     [+ o -] 0.015     [+ o -] 0.028
H-W P (2)                  0.978             0.001             0.792
                   [+ o -] 0.0004    [+ o -] 0.0001    [+ o -] 0.001

                      Total            Chorotega         Filipino
Allele (1)           Chibcha            Matambu        Panai Island

141 (10)                   0.003                               0.071
                   [+ o -] 0.002                       [+ o -] 0.02
136 (9)                    0.387             0.487             0.107
                   [+ o -] 0.014     [+ o -] 0.041     [+ o -] 0.024
131 (8)                    0.089             0.127             0.292
                   [+ o -] 0.008     [+ o -] 0.027     [+ o -] 0.035
126 (7)                    0.506             0.353             0.441
                   [+ o -] 0.015     [+ o -] 0.039     [+ o -] 0.038
121 (6)                    0.011             0.033             0.03
                   [+ o -] 0.003     [+ o -] 0.015     [+ o -] 0.013
116 (5)                    0.004                               0.06
                   [+ o -] 0.002                       [+ o -] 0.018
Observed                   0.526             0.693             0.714
Heterozygosity
Gene                       0.587             0.625             0.704
Diversity          [+ o -] 0.077     [+ o -] 0.022     [+ o -] 0.023
H-W P (2)                  0.003             0.763             0.924
                   [+ o -] 0.0001    [+ o -] 0.001     [+ o -] 0.001

(1) Allele size in bp (number of copies of the repeat).

(2) Hardy-Weinberg fit (probability values).

TABLE 4

Allele frequencies, observed and expected heterozygosities, and
Hardy-Weinberg equilibrium test of the STR [(AAAAT).sub.n] within
intron 1 of the TP53 locus in Africans, Costa Ricans, Germans, Turks
and Portuguese

                                     Negroid

                    African          African           Afro-
Allele (1)          Namibia          Uganda         Costa Rica

151 (12)                 0.015            0.006
                 [+ o -] 0.009    [+ o -] 0.006
146 (11)                 0.005            0.012            0.008
                 [+ o -] 0.005    [+ o -] 0.009    [+ o -] 0.005
141 (10)                 0.101            0.056            0.042
                 [+ o -] 0.022    [+ o -] 0.018    [+ o -] 0.01
136 (9)                  0.187            0.136            0.241
                 [+ o -] 0.028    [+ o -] 0.027    [+ o -] 0.022
131 (8)                  0.253            0.321            0.319
                 [+ o -] 0.031    [+ o -] 0.037    [+ o -] 0.024
126 (7)                  0.318            0.37             0.311
                 [+ o -] 0.033    [+ o -] 0.038    [+ o -] 0.024
121 (6)                  0.03             0.025            0.044
                 [+ o -] 0.012    [+ o -] 0.012    [+ o -] 0.011
116 (5)                  0.091            0.056            0.036
                 [+ o -] 0.021    [+ o -] 0.018    [+ o -] 0.01
111 (4)                                   0.019
                                  [+ o -] 0.011
Observed                 0.829            0.728            0.741
Heterozygosty
Gene                     0.784            0.739            0.741
Diversity        [+ o -] 0.013    [+ o -] 0.02     [+ o -] 009
H-W P (3)                0.039            0.828            0.324
                 [+ o -] 0.001    [+ o -] 0.001    [+ o -] 0.001

                    Negroid                  Caucasoid

                    Hybrid          European           Asian
Allele (1)        Costa Rica         Germany          Turkey

151 (12)
146 (11)                 0.005
                 [+ o -] 0.003
141 (10)                 0.032            0.01
                 [+ o -] 0.006    [+ o -] 0.004
136 (9)                  0.28             0.236            0.256
                 [+ o -] 0.016    [+ o -] 0.019    [+ o -] 0.033
131 (8)                  0.372            0.62             0.58
                 [+ o -] 0.017    [+ o -] 0.021    [+ o -] 0.037
126 (7)                  0.279            0.103            0.136
                 [+ o -] 0.016    [+ o -] 0.013    [+ o -] 0.026
121 (6)                  0.021            0.032            0.028
                 [+ o -] 0.005    [+ o -] 0.008    [+ o -] 0.013
116 (5)                  0.011
                 [+ o -] 0.004
111 (4)

Observed                 0.688            0.533            0.613
Heterozygosty
Gene                     0.705            0.55             0.583
Diversity        [+ o -] 0.006    [+ o -] 0.019    [+ o -] 0.029
H-W P (3)                0.002            0.771            0.329
                 [+ o -] 0.001    [+ o -] 0.001    [+ o -] 0.002

                   Caucasoid

                   European
Allele (1)       Portugal (2)

151 (12)
146 (11)                 0.002
141 (10)                 0.01
136 (9)                  0.287
131 (8)                  0.496
126 (7)                  0.131
121 (6)                  0.064
116 (5)                  0.01
111 (4)
Observed                 0.635
Heterozygosty
Gene                     0.651
Diversity
H-W P (3)                0.447
                 [+ o -] 0.001

(1) Allele size in by (number of copies of the repeat).

(2) Data from Gusmao et al. (1997).

(3) Hardy-Weinberg fit (probability values).

TABLE 5

Genetic distances (X100) based on the [(AAAAT).sub.n] polymorphism
within intron 1 of the TP53 locus in ten populations

                Chibcha    Mesoamerican    Filipino    Namibia

Mesoamerican      2.601
Filipino         10.641       13.427
Namibia           9.462        8.192         1.119
Uganda           11.361       11.631         0.000      0.345
Afro-CR           9.112        6.754         2.117      0.565
German           40.592       32.150        20.537     15.996
Turks            33.407       24.749        15.468     11.317
Portuguese       26.250       17.152        12.963      8.407
Costa Rica       11.274        7.539         4.364      2.188

                Uganda    Afro-CR    German    Turks    Portuguese (1)

Mesoamerican
Filipino
Namibia
Uganda
Afro-CR          0.639
German          14.472     10.608
Turks           10.261      6.970    0.000
Portuguese       8.305      4.711    1.462     0.365
Costa Rica       2.081      0.277    7.228     4.421        2.7421

(1) From Gusmao et al. (1997).

TABLE 6

Likelihood ratio tests for step-wise mutational mechanisms related to
the STR [(AAAAT).sub.n] within intron 1 of the TP53 locus in ten
populations

                            One-step           Multi-step

                        q      1([theta])      q       q

Mongoloid
  Chibcha              2.41      -28.30       1.86    0.08
  Mesoamerican         1.50      -14.99       1.50    0.01
  Filipino             5.01      -21.82       5.01    0.01
Negroid
  Namibia              8.07      -28.80      11.28    0.01
  Uganda               7.88      -29.34      10.49    0.01
  Costa Rica           5.81      -27.98       7.25    0.01
Caucasoid
  German               2.38      -20.52       3.23    0.01
  Portuguese           5.49      -27.39       2.37    0.07
  Turk                 2.12      -14.19       2.69    0.01
Hybrid
  Costa Rica           4.02      -31.73       6.97    0.01

                        Multi-step

                       1([theta]q)    [[ji al cuadro].sub.1]      p

Mongoloid
  Chibcha                -28.53                0.00              1.00
  Mesoamerican           -15.09                0.00              1.00
  Filipino               -22.00                0.00              1.00
Negroid
  Namibia                -29.40                0.00              1.00
  Uganda                 -26.69                0.00              1.00
  Costa Rica             -27.79                0.38              0.54
Caucasoid
  German                 -20.40                0.24              0.62
  Portuguese             -29.33                0.00              1.00
  Turk                   -14.23                0.00              1.00
Hybrid
  Costa Rica             -31.03                1.40              0.24

[[ji al cuadro].sub.1] = -2 log [L([theta],q=0)/L([theta],q)], [theta]
= 4Ne[my] , q = estimated proportion of multi-step mutations (Nielsen
1997).
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Article Details
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Author:Azofeifa, Jorge; Hahn, Meinhard; Ruiz, Edward; Hummerich, Lars; Morales, Ana I.; Jimenez, Gerardo; B
Publication:Revista de Biologia Tropical
Date:Sep 1, 2004
Words:6866
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