The PV-92 Alu element. (Senior Division).
The purpose of this project was to show the correlation between the
evolution of the PV-92 Alu element and the common ancestry of members of
the Woodlin community. I hypothesized that 50% of the volunteers would
test heterozygous for the PV-92 Alu element, showing a correlation
between related volunteers. Use Chelex[R] resin to isolate DNA from
cheek cells. Amplify DNA by PCR. Analyze DNA by gel electrophoresis. The
PV-92 Alu element was present on at least one allele in 50% of the
volunteers tested. However, not all of these volunteers were
heterozygous. Volunteers one and four were homozygous for PV-92.
Volunteers three, five, ten, twelve, and thirteen were heterozygous for
PV-92. Volunteers two, six, seven, eight, nine, and eleven were
homozygous for the absence of PV-92. Volunteers five, twelve, and
thirteen were all related and tested heterozygous for PV-92. This
confirmed the second part of my hypothesis. The first part of my
hypothesis was not confirmed, because 50% of the volunteers tested were
not heterozygous for PV-92. I contribute this to the small size of the
volunteer group used in my experiment. A larger group might have shown
numbers closer to those of the general population.
Holly McGuire, Woodlin High School.