The Association between Earlobe Crease (Frank's Sign) and Abnormal Ankle-Brachial Index Determination Is Related to Age: A Population-Based Study.
Earlobe crease (ELC), also known as Frank's sign, is a wrinkle extending from the tragus to the outer border of the earlobe . Studies have linked ELC with cardiovascular events [2, 3]. However, others believe that ELC may be an unrelated bystander . ELC has also been associated with increased carotid artery intima-media thickness and arterial stiffness . These findings have opened new venues for the use of ELC for identifying apparently healthy individuals at risk of developing vascular events.
A recent study showing that ELC may detect asymptomatic individuals with peripheral artery disease (PAD) is promising , since untreated PAD has been associated with foot ulcers, lower limb amputations, cardiovascular events, and all-cause mortality . However, information on the predictive value of ELC for detection of subjects with PAD is limited and may not be representative of the population at large. Here, we aimed to assess the relationship between ELC and abnormal ABI determinations in community-dwelling older adults living in rural Ecuador (Atahualpa).
2. Material and Methods
Atahualpa represents an optimal setting for the practice of epidemiological studies. As previously described, villagers are homogeneous regarding race/ethnicity, lifestyle, socioeconomic status, and diet . The IRB of Hospital-Clinica Kennedy, Guayaquil, Ecuador (FWA 00006867), approved the protocol and the informed consent. All Atahualpa residents aged [greater than or equal to] 60 years were invited to participate for evaluating the association between the presence and characteristics of ELC and results of ABI determinations, after adjusting for relevant confounders.
Both earlobes were examined with the subject in the sitting position. According to Rodriguez-Lopez et al. , an ELC was considered to be present when the individual has a wrinkle (of different shapes and length) extending from the tragus to the outer border of the earlobe (Figure 1). Subjects with creases related to earrings and those who have physical damage distorting earlobe anatomy were excluded. Two investigators, blinded to each other's assessments and to clinical data assessed the earlobes. Interrater agreement was adequate for ELC presence (kappa=0.95), and discrepancies were resolved by consensus.
ABI determinations followed the recommendations of the American Heart Association . A manual sphygmomanometer and a portable vascular Doppler with an 8 MHz probe were used for blood pressure (BP) measurements. The ABI was calculated by dividing the highest of the posterior tibial or dorsalis pedis BP in each leg by the higher arm systolic BP (right or left). The lowest ABI between the two ankles was used for classifying purposes. ABI determinations <0.9 and [greater than or equal to] 1.4 were considered abnormal, the former reflecting PAD and the latter calcinosis.
Demographics and cardiovascular risk factors, selected as confounding variables, were assessed through interviews and procedures previously described in the Atahualpa Project. These included age, sex, smoking status, physical activity, diet, the body mass index, BP, fasting glucose, and total cholesterol blood levels .
Data analyses are carried out by using STATA version 14 (College Station, TX, USA). In univariate analyses, continuous variables were compared by linear models and categorical variables by [x.sup.2] or Fisher exact test as appropriate. Using logistic regression models, we evaluated whether ELC was associated with an abnormal ABI (dependent variable), after adjusting for demographics and cardiovascular risk factors. We anticipated a potential effect modification of age in the association between ELC and abnormal ABI determinations and fitted multivariate probability models with participants stratified according to the median age of the population.
Of 437 Atahualpa residents aged [greater than or equal to] 60 years identified during door-to-door surveys, 294 (67%) underwent ABI determination and earlobe examinations. Of the 143 nonincluded individuals, earlobe deformities did not allow characterization of ELC in 14, and the ABI could not be obtained in 72 cases because of refusal to consent (n=57), leg amputations (n=10), and uncontrolled tremor in the legs (n=5). In addition, 57 subjects had died or emigrated between enrollment and the invitation.
Table 1 shows the characteristics of participants across categories of ABI. As noted, individuals with abnormal ABI determinations were older, had worse physical activity, and had more often BP levels [greater than or equal to] 140/90 mmHg than those with normal ABIs. Regarding ELC, univariate analysis showed a nonsignificant trend for being more frequent among individuals with abnormal ABI determinations (p=0.068).
A logistic regression model, adjusted for demographics and cardiovascular risk factors, revealed no significant association between ELC and abnormal ABI determinations (OR: 1.71; 95% CI: 0.89 - 3.26; p=0.104). In this model, the only significant covariate was age (p<0.001). Probability margins of abnormal ABI determinations, with individuals stratified according to their median age (69 years), showed that probabilities of abnormal ABI determinations nonsignificantly increased among older individuals with ELC (Figure 2).
Results of this study suggest that the association between ELC and abnormal ABI determinations could be attributed to age. These findings might be explained by the high prevalence of both variables in older adults, confirming previous hypotheses that ELC and atherosclerosis could be related to the effects of aging [4,11].
In a study aimed at correlating ELC with ABI determination , 136 of 253 (54%) individuals free of symptomatic atherosclerosis had ELC, and ABI determinations were significantly lower than in those without ELC. However, the authors used a different ABI cutoff value for defining PAD and results may not be comparable with our findings. Moreover, the influence of age in the relationship between ELC and abnormal ABI determinations was not specifically investigated. Yet, in another study, the authors found no significant association between the presence of an ELC and peripheral artery disease .
There is limited information about pathogenetic mechanisms explaining how ELC might correlate with atherosclerosis. In an autopsy-based report, it was suggested that "one of the underlying pathogenetic mechanisms involved in the progression of atherosclerosis, possible related to collagen metabolism, may also occur in the skin" . It has also been suggested that the ELC might be genetically determined and related to the occurrence of atherosclerosis, which might explain its different prevalence according to race/ethnicity .
The present study has limitations. Results may not be generalized to other races/ethnic groups but are of value for Amerindians, a population where the association between ELC and atherosclerosis has not been previously investigated. In addition, we cannot rule out the presence of some hidden confounders (such as asymptomatic coronary artery disease), which can modify the final results obtained in the multivariate model. Also, the possibility of a statistical type 2 error cannot be completely ruled out, because the statistical power of our sample was 57.5%. Major strengths include the population-based design with unbiased enrollment of participants and the models used to evaluate the influence of age on the association between ELC and abnormal ABI determinations.
This study suggests that the association between ELC and abnormal ABI determinations is attenuated by the high prevalence of both conditions in older persons. ELC might not be useful for identifying candidates for ABI determination.
The data is confidential according to the policy of the authors' institution.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
This study was supported by Universidad Espiritu Santo Ecuador.
 S. T. Frank, "Aural Sign of Coronary-Artery Disease," The New England Journal of Medicine, vol. 289, no. 6, pp. 327-328, 1973.
 H. Shmilovich, V. Y. Cheng, R. Rajani et al., "Relation of diagonal ear lobe crease to the presence, extent, and severity of coronary artery disease determined by coronary computed tomography angiography," American Journal of Cardiology, vol. 109, no. 9, pp. 1283-1287, 2012.
 M. Christoffersen, R. Frikke-Schmidt, P. Schnohr, G. B. Jensen, B. G. Nordestgaard, and A. Tybjoerg-Hansen, "Visible age-related signs and risk of ischemic heart disease in the general population: A prospective cohort study," Circulation, vol. 129, no. 9, pp. 990-998, 2014.
 I. A. Gutiu, E. Galetescu, and L. I. Gutiu, "Diagonal earlobe crease: a coronary risk factor, a genetic marker of coronary heart disease, or a mere wrinkle. Ancient Greco-Roman evidence," Romanian Journal of Internal Medicine, vol. 34, pp. 217-278, 1996.
 P. Rerknimitr, K. Pongpairoj, C. Kumtornrat et al., "Anterior Tragal Crease Is Associated with Atherosclerosis: A Study Evaluating Carotid Artery Intima-Media Thickness," Angiology, vol. 68, no. 8, pp. 683-687, 2017.
 L. Korkmaz, M. T. Agac, Z. Acar et al., "Earlobe crease may provide predictive information on asymptomatic peripheral arterial disease in patients clinically free of atherosclerotic vascular Disease," Angiology, vol. 65, no. 4, pp. 303-307, 2014.
 V. Aboyans, M. H. Criqui, P. Abraham et al., "Measurement and interpretation of the ankle-brachial index: a scientific statement from the American Heart Association," Circulation, vol. 126, no. 24, pp. 2890-2909, 2012.
 O. H. Del Brutto, E. Penaherrera, E. Ochoa, M. Santamaria, M. Zambrano, and V. J. Del Brutto, "Door-to-door survey of cardiovascular health, stroke, and ischemic heart disease in rural coastal Ecuador--the Atahualpa Project: Methodology and operational definitions," International Journal of Stroke, vol. 9, no. 3, pp. 367-371, 2014.
 C. Rodriguez-Lopez, H. Garlito-Diaz, R. Madronero-Mariscal et al., "Earlobe Crease Shapes and Cardiovascular Events," American Journal of Cardiology, vol. 116, no. 2, pp. 286-293, 2015.
 O. H. Del Brutto, M. Santamaria, E. Ochoa et al., "Population-based study of cardiovascular health in Atahualpa, a rural village of coastal Ecuador," International Journal of Cardiology, vol. 168, no. 2, pp. 1618-1620, 2013.
 P. M. Brady, M. A. Zive, R. J. Goldberg, J. M. Gore, and J. E. Dalen, "A New Wrinkle to the Earlobe Crease," JAMA Internal Medicine, vol. 147, no. 1, pp. 65-66,1987.
 D. Moraes, P. McCormack, J. Tyrrell, and J. Feely, "Ear lobe crease and coronary heart disease.," Irish Medical Journal, vol. 85, no. 4, pp. 131-132, 1992.
 V. Patel, C. Champ, P. S. Andrews, B. E. Gostelow, N. P. Gunasekara, and A. R. Davidson, "Diagonal earlobe creases and atheromatous disease: a postmortem study.," Journal of the Royal College of Physicians, vol. 26, no. 3, pp. 274-277,1992.
Oscar H. Del Brutto, (1) Robertino M. Mera, (2) Aldo F. Costa, (1) Mauricio Zambrano, (3) and Mark J. Sedler (4)
(1) School of Medicine, Universidad Espiritu Santo-Ecuador, Guayaquil, Ecuador
(2) Vanderbilt University Medical Center, Nashville, TN, USA
(3) Community Center, The Atahualpa Project, Atahualpa, Ecuador
(4) School of Medicine, Stony Brook University, New York, NY, USA
Correspondence should be addressed to Oscar H. Del Brutto; email@example.com
Received 27 April 2018; Revised 31 July 2018; Accepted 9 August 2018; Published 2 September 2018
Academic Editor: Robert M. Schainfeld
Caption: FIGURE 1: Diagonal earlobe crease in an Atahualpa resident.
Caption: FIGURE 2: Probability margins of abnormal ankle index (ABI) determination according to earlobe crease (ELC) presence, with participants stratified by the median age of the population (69 years). In both age groups, the probability of abnormal ABI is higher among individuals with an ELC, but differences are not significant due to overlapping 95% confidence intervals.
Table 1: Characteristics of Atahualpa residents aged [greater than or equal to] 60 years across categories of ankle-brachial index determinations (univariate analyses). Total series (n=294) Normal ABI (n=238) Age, years (mean 70.9 [+ or -] 7.9 69.6 [+ or -] 6.9 [+ or -] SD) Women, n (%) 171 (58.2) 138 (58) Current smokers, n (%) 4 (1.4) 4 (1.7) Body mass index [greater 64 (21.8) 53 (22.3) than or equal to] 30 kg/[m.sup.2], n (%) Poor physical activity, 23 (7.8) 14 (5.9) n (%) Poor diet, n (%) 13 (4.4) 8 (3.4) Blood pressure [greater 131 (44.6) 99 (41.6) than or equal to] 140/90 mmHg, n (%) Fasting glucose [greater 96 (32.7) 78 (32.8) than or equal to] 126 mg/dL, n (%) Total cholesterol [greater 37 (12.6) 27 (11.3) than or equal to] 240 mg/dL, n (%) Presence of earlobe 141 (48) 108 (45.4) crease, n (%) Abnormal ABI (n=56) p value Age, years (mean 76.5 [+ or -] 9.3 <0.001 [+ or -] SD) Women, n (%) 33 (58.9) 0.897 Current smokers, n (%) 0 0.741 Body mass index [greater 11 (19.6) 0.668 than or equal to] 30 kg/[m.sup.2], n (%) Poor physical activity, 9 (16.1) 0.011 n (%) Poor diet, n (%) 5 (8.9) 0.079 Blood pressure [greater 32 (57.1) 0.035 than or equal to] 140/90 mmHg, n (%) Fasting glucose [greater 18 (32.1) 0.928 than or equal to] 126 mg/dL, n (%) Total cholesterol [greater 10 (17.9) 0.671 than or equal to] 240 mg/dL, n (%) Presence of earlobe 33 (58.9) 0.068 crease, n (%)
|Printer friendly Cite/link Email Feedback|
|Title Annotation:||Research Article|
|Author:||Del Brutto, Oscar H.; Mera, Robertino M.; Costa, Aldo F.; Zambrano, Mauricio; Sedler, Mark J.|
|Publication:||International Journal of Vascular Medicine|
|Date:||Jan 1, 2018|
|Previous Article:||Comparative Study of Protein Expression Levels of Five Plaque Biomarkers and Relation with Carotid Plaque Type Classification in Patients after...|
|Next Article:||Comment on "Effect of Exercise Intervention on Flow-Mediated Dilation in Overweight and Obese Adults: Meta-Analysis".|