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Sustained Viremic Control in HIV-infected Patient: Case Report from Nepal.

1. General Background

The incidence of HIV infection is increasing globally. WHO reported 34 million people living with HIV at the end of 2011 [1]. The prevalence of HIV infection among adults was reported to be 0.20% in the year 2014 in Nepal [2]. It is estimated that 7,400 new cases of HIV are added each day worldwide. In the year 2011, 1.7 million deaths occurred due to HIV-AIDS, and in the same year, 2.5 million new cases were diagnosed of HIV-AIDS [3].

Many attempts have been made to control as well as provide successful treatment to the deadly disease, yet there is not an easy-to-follow strict regimen for treatment. One of the major causes of the situation the unclear understanding about the various complicated modes of HIV pathogenesis. Different research works suggest that the previously known and newly emerging HIV pathogenesis is somehow different due to which some people can constantly control over viral replication thus resulting in long-term nonprogressors and elite controllers [4].

Elite controllers are those HIV-positive individuals that show plasma HIV-RNA value persistently below 50 copies/ml or below the lower detection threshold of clinical assays without antiretroviral therapy throughout the course of infection or for at least 12 months [4-7]. On the other hand, long-term nonprogressors are those individuals showing low-detectable plasma viremia, that is, less than 5000 HIV-RNA copies/ml [4].

Here, we presented a case that has an extraordinary control over HIV replication with constant maintenance of CD4 count and HIV-RNA level below the lower detection threshold for more than 10 years. The case was identified in 2016 by retrospective analysis of the laboratory report and clinical history explained by the patient. Though this type of case has been reported from developed countries, to our knowledge, this is the first case report from a developing country like Nepal.

This type of case is important because this may aid in understanding the pathogenesis of HIV in elite controllers that is still under investigation. Furthermore, these candidates may be used for therapeutics and vaccine development which may contribute to the establishment of definite treatment guidelines for HIV-related cases.

2. Case Description

A 40-year-old male resident from Kathmandu, Nepal, was tested positive for HIV infection by the ELISA method in 2008. In addition to ELISA, supplementary testing for HIV serology was done by using rapid test kits (Determine, Uni-Gold, and Stat-Pak) which are recommended by national as well as WHO guidelines for confirmation. In the described case, all the three tests were positive; hence, the case was considered as HIV infected and requested for follow-up clinic visit for CD4 testing, viral load, and opportunistic infections before initiation of antiretroviral therapy.

He shared that he was engaged in needle sharing while injecting drugs, possibly a mode of transmission. In the initial finding, CD4 count was 450 cells/[micro]l, and the viral load was less than the detectable limit. He had his CD4 count performed every year since 2008 and viral load testing, which was always below lower detection threshold. The detailed data is presented in Table 1. In 2016, western blot investigation was performed after finding of strikingly continuous undetectable viral load and high CD4 count to reassess the HIV status. The result of HIV western blot was positive. Until this time, the patient was not enrolled on ART. The patient had not any kinds of opportunistic infection till date and had not suffered from any kinds of serious illness. He is married now, and his partner is HIV negative till now.

All the tests were performed at National Public Health Laboratory (NPHL), Teku, Kathmandu. CD4 count was performed using BD fluorescent-activated cell sorter system (BD Biosciences, San Jose, CA, USA). For this, whole blood sample was collected in EDTA vial, and the count was performed as per the manufacturer's instructions. For viral load testing, whole blood sample was collected, and plasma was used for further testing. Viral RNA was extracted using QIAamp Viral RNA Mini Kit or Roche's High Pure Viral Nucleic Acid Kit. The extracted RNA was then analyzed by real time polymerase chain reaction (RT-PCR) using Corbett Rotor-Gene 6000, COBAS[R]TaqMan[R] 48 Analyzer.

3. Discussion

Elite controllers are very rare group of populations harboring human immunodeficiency virus. It is known that such type of population possess HIV virus but could not transmit infection to other individuals. The mechanism by which they could control viral replication has not been fully understood till date [4, 8, 9]. Some studies suggest that the host immune factor plays an important role in controlling replication while others suggest that it is the result of infection with defective viral strain [10]. Finding such type of cases may aid in understanding the mechanism involved in the control of HIV replication in them. This may be useful in the development of therapeutics and vaccines of HIV-AIDS [6].

In this report, we present a case who had sustained viremic control over HIV infection and maintenance of plasma RNA viral load below 50 copies/ml for more than 10 years. The strong evidence in identifying this case is the laboratory report of viral load and CD4 count with the patient. We found that during the course of 10 years, the patient had constantly maintained the plasma HIV-RNA viral load less than 50 copies/ml and possessed increasing slope of CD4 count (Table 1 and Figure 1). We noted that the patient had decreasing slope of CD4 count in the years 2011 and 2012 though the HIV-RNA viral load was less than 50 copies/ml. We also noted that during such period, the patient had comparatively high percent of CD4 cells. This might be due to some kinds of infection in the patient that have decreased the total WBC count and CD4 count as well.

Several studies have been performed in developed countries regarding elite controllers as well as pathogenesis and host factors but yet have not been able to find any kind of single case from a developing country like Nepal. Probably, this is the first case reporting the evidence of an elite controller in Nepal.

4. Conclusions

Detection of elite controllers in HIV-infected patients is a boon for such patients as they could live a normal life without antiretroviral therapy despite HIV infection. On the other hand, this type of cases added further challenges to the researcher in understanding the pathogenesis of HIV as well as other factors responsible for sustained control of viremia in HIV-infected patients. Furthermore, this type of individuals could be used in near future for the development of vaccines and other therapeutics.

Consent

Written consent was obtained from the patient for publication of this case report. https://doi.org/10.1155/2018/5279595

Conflicts of Interest

The authors declare they have no conflicts of interest.

Authors' Contributions

All authors read and approved the final manuscript.

References

[1] World Health Organization, Antimicrobial Resistance Global Report on Surveillance: 2014 Summary, WHO, Geneva, Switzerland, 2014.

[2] T. Paudel, N. Singh, M. R. Banjara et al., "Epidemiology of HIV, programmatic progress and gaps in last 10 years in Nepal," Journal of Virus Eradication, vol. 2, no. 4, pp. 35-40, 2016.

[3] World Health Organization, Urinary Tract Infections in Infants and Children in Developing Countries in the Context of IMCI, WHO, Geneva, Switzerland, 2005.

[4] N. K. Saksena, B. Rodes, B. Wang, and V. Soriano, "Elite HIV controllers: myth or reality?," AIDS Reviews, vol. 9, no. 4, pp. 195-207, 2007.

[5] A. Rachinger, M. Navis, S. van Assen, P. H. Groeneveld, and H. Schuitemaker, "Recovery of viremic control after super-infection with pathogenic HIV type 1 in a long-term elite controller of HIV type 1 infection," Clinical Infectious Diseases, vol. 47, no. 11, pp. e86-e89, 2008.

[6] B. D. Walker, "Elite control of HIV infection: implications for vaccines and treatment," Topics in HIV Medicine, vol. 15, no. 4, pp. 134-136, 2007.

[7] D. Mendoza, S. A. Johnson, B. A. Peterson et al., "Comprehensive analysis of unique cases with extraordinary control over HIV replication," Blood, vol. 119, no. 20, pp. 4645-4655, 2012.

[8] M. Salgado, S. A. Rabi, K. A. O'Connell et al., "Prolonged control of replication-competent dual-tropic human immunodeficiency virus-1 following cessation of highly active antiretroviral therapy," Retrovirology, vol. 8, no. 1, p. 97, 2011.

[9] S. Gaillard, J. B. Dinoso, J. A. Marsh et al., "Sustained elite suppression of replication competent HIV-1 in a patient treated with rituximab based chemotherapy," Journal of Clinical Virology, vol. 51, no. 3, pp. 195-198, 2011.

[10] R. W. Buckheit III, T. G. Allen, A. Alme et al., "Host factors dictate control of viral replication in two HIV-1 controller/chronic progressor transmission pairs," Nature Communications, vol. 3, no. 1, p. 716, 2012.

Sundar Khadka (iD), (1) Subhash Dhital (iD), (1) Roshan Pandit, (1) and Anup Bastola (2)

(1) HIV Reference Unit, National Public Health Laboratory, Kathmandu, Nepal

(2) Sukraraj Tropical & Infectious Disease Hospital, Teku, Kathmandu, Nepal

Correspondence should be addressed to Sundar Khadka; cls.sundar@gmail.com

Received 15 January 2018; Accepted 18 February 2018; Published 13 March 2018

Academic Editor: Paola Di Carlo

Caption: Figure 1: Timeline showing CD4 cell count and its percentage.
Table 1: Result of the patient's test report.

Tests             2008     2009   2010   2011   2012   2013   2014

ELISA *         Positive    ND     ND     ND     ND     ND     ND
PCR-RNA            ND      <34    TND    TND    <34    TND    TND
Western blot       ND       ND     ND     ND     ND     ND     ND

Tests           2015     2016     2017

ELISA *          ND       ND       ND
PCR-RNA         TND      <34      <34
Western blot     ND    Positive    ND

TND = target not detected; ND = test not done. * Supplementary testing
was done along with ELISA before confirmation.
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Title Annotation:Case Report
Author:Khadka, Sundar; Dhital, Subhash; Pandit, Roshan; Bastola, Anup
Publication:Case Reports in Infectious Diseases
Date:Jan 1, 2018
Words:1598
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