Study on the Role of the Inclusion Complexes with 2-Hydroxypropyl-[beta]-cyclodextrin for Oral Administration of Amiodarone.
Amiodarone hydrochloride (AMD) is a highly effective antiarrhythmic drug used in the management of severe ventricular and supraventricular arrhythmias . It is considered a class II antiarrhythmic drug according to Vaughan Williams' classification, but it possesses multiple electrophysiological effects (the characteristics of all four Vaughan Williams classes). Although it generates pulmonary and hepatic toxicity and thyroid dysfunction, AMD remained the most efficient drug for the treatment of a wide variety of arrhythmias because it has demonstrated high clinical efficacy including for those complicated with Wolff-Parkinson-White syndrome or refractory to adequate doses of other antiarrhythmic drugs [2, 3].
The use of AMD, a highly lipophilic drug, is complicated by its complex pharmacokinetics . The main therapeutic disadvantage of AMD is its low oral bioavailability and low water solubility; therefore, intravenously administered amiodarone is preferred in the acute treatment. The absorption of orally administrated AMD is slow and variable, with peak plasma concentrations that occur at 2 to 7 hours following a single oral dose. Oral bioavailability is extremely variable and ranges from 22 to 86%. However, if AMD is ingested with fatty foods, the rate and extent of absorption are increased . The plasma levels resulting from orally administered AMD vary considerably between individuals, while efficacy has been observed for AMD plasma concentrations as low as 0.1 [micro]g/mL, other studies showed therapeutic efficacy for AMD plasma concentration of 1-2.5 [micro]g/mL [6, 7]. The low bioavailability may be related to intestinal wall metabolism mediated by CYP3A4 enzyme and gastrointestinal excretion mediated by P-glycoprotein. After absorption, AMD undergoes extensive enterohepatic circulation before being distributed to the central compartment and tissues. The maximum time ([t.sub.max]) from oral intake to reaching peak plasma concentration ([C.sub.max]) after a single-dose administration ranges from 2 to 10 h. AMD is eliminated primarily by hepatic metabolism and biliary excretion.
N-Desethylamiodarone is the CYP3A4 metabolite of amiodarone identified in plasma, which also suppressed ventricular arrhythmias [6, 7]. The low water solubility of AMD extensive first pass effect might be other reasons for its large interindividual variation in systemic bioavailability. Moreover, AMD presents poor dissolution characteristics, which is one of the main factors limiting its gastrointestinal absorption. As it was mentioned, due to its slow dissolution rate and high membrane permeability, AMD is classified as a class II drug based on the Biopharmaceutical Classification System [8, 9].
Low solubility affects the pharmacokinetic properties of the active compounds; therefore, some solutions have been proposed, as complexation or conjugation with cyclodextrin as a method to optimize the therapeutic performance of the insoluble drugs including AMD [10-13].
Cyclodextrins (CDs) are cyclic oligosaccharides containing at least 6 D-(+) glucopyranose units attached by [alpha]-1,4-glycosidic bonds. They contain a hydrophobic cavity in which the active compound is internalized forming an inclusion complex with the drug. Both inclusion complex formation and its dissociation occur in very short time ([t.sub.1/2] < 1 sec) , and such processes are generally expected to be much faster than many physiological processes . CDs have significant influence on drug dissolution, bioavailability, safety, and stability, and therefore, they are increasingly used as excipients in drug formulations . CDs are also widely used in the pharmaceutical applications because of their ready availability and cavity size suitable for the widest variety of drugs. However, the low aqueous solubility and nephrotoxicity limited the use of [beta]-CD especially in parenteral drug delivery. Chemically modified CD or their derivatives have been prepared to extend the physicochemical properties (such as enhanced aqueous solubility, physical and microbiological stability, and reduced parenteral toxicity) and inclusion capacity of parent CDs [17, 18].
The hydroxypropyl-[beta]-cyclodextrin (HP-[beta]-CD) shows the best solubility (500 mg/mL) and lowest toxicity . From the pharmaceutical point of view, the HP-[beta]-CD presents the following advantages: increases the active substance solubility, bioavailability, and dissolution rate; reduces the side effects; and contributes to the stabilization of the pharmaceutical formulations . Inclusion complexes of HP-[beta]-CD are of great interest in pharmaceutical application, and they have been used to increase the solubility of a great number of various drugs [16, 21-26]. Rubim et al.  followed the effect of the CD type as [beta]-cyclodextrin, methyl-[beta]-cyclodextrin, and 2-hydroxypropyl-[beta]-cyclodextrin on the complexation with AMD and release ability. The enhanced solubility and dissolution may help to improve in vivo performance. Only a few studies are reported on the formation and characterization of inclusion complexes of HP-[beta]-CD with amiodarone , but no detailed studies on loading and release of the drug have been found, and there are no pharmacokinetic data available about these systems.
In our previous papers, it was established that the dissolution rate of amiodarone from the inclusion complex was considerably increased as compared to dissolution of the pure drug. It has been established that the complexation of amiodarone with HP-[beta]-CD offers the possibility to increase its water solubility without the modification of its original structure [28-31]. It is well known that the type of [beta]-CD can influence the formation as well as the performance of drug/[beta]-CD complexes [32, 33].
In the present study, the effect of the substitution degree and molecular weight of the 2-hydroxypropyl-[beta]-cyclodextrin on the inclusion complex formation with the amiodarone and their physicochemical properties have been followed; also, the ability of loading and controlled release was studied by in vitro studies. The objective of this research was to evaluate the AMD bioavailability from the inclusion complexes as a formulation for oral administration with modified release and with optimal biopharmaceutical properties. Therefore, the second part of the paper reports some pharmacotechnical studies, where the inclusion complex with the best solubility was included in matrix tablets in order to obtain a modified-release behavior. In this respect, two formulations of matrix tablets were developed: one containing the inclusion complex with the optimal solubility and one containing only pure AMD as a control sample. The kinetics of AMD release from the two formulations was evaluated by in vitro and in vivo studies.
The disadvantage of oral administration is the extremely slow absorption of AMD from the oral forms and also low and variable bioavailability. The optimization of the pharmacokinetic properties was achieved by incorporation of the inclusion complexes in matrix tablets containing hydrophilic polymers such as Kollidon[R] SR (KOL) and chitosan (CHT) as excipients; finally, therapeutic systems with controlled prolonged release for oral delivery have been obtained.
The solubility enhancement of AMD by the inclusion in complexes with HP-[beta]-CD was of 4-22 times. Depending on HP-[beta]-CD amount in the complex composition at a faster dissolution rate with small difference concerning the HP-[beta]-CD type was found. The formulations of AMD/HP-[beta]-CD inclusion complexes both as powdered form and matrix tablets showed superior pharmacokinetic performance in improving loading and release properties of the insoluble AMD drug. An in vitro kinetic dissolution test reveals a complex mechanism occurring in three steps: the first one being attributed to a burst effect and the other two to different bonding existing in inclusion complexes. In vivo pharmacokinetic study from matrix tablets containing Kollidon[R] and chitosan also gave a multiple (at least two) peaks release diagram because of both structures of the inclusion complexes and also of different sites of absorption in biological media (digestive tract).
2.1. Materials. AMD (Mw = 645.32 Da) of 99.85% purity was delivered by Zhejiang Sanmen Hengkang Pharmaceutical Co. Ltd., China. The three hydroxypropyl-[beta]-cyclodextrins (HP-[beta]-CDs) of 99.70% purity and with different substitution degrees and average molecular weights (Mw) have been obtained from Roquette, France. Their characteristics are given in Table 1.
Polysorbate 80 with 99.50% purity was purchased from Sigma-Aldrich, Germany. It was used as a nonionic surfactant and emulsifier for AMD suspension.
Kollidon[R] SR (KOL) is a physical mixture constituted from 80% poly(vinyl acetate) with an average molecular weight (Mw) of 450000 Da and 20% polyvinylpyrrolidone (povidone) of Mw = 40000 Da . KOL is a hydrophilic excipient used in formulations and preparation of the matrix tablets with modified-release dosage (delayed, prolonged, or targeted release) being both efficient and versatile .
Chitosan (CHT) with high molecular weight Mw = 190000-375000 Da and deacetylation degree [greater than or equal to] 75 of practical grade was purchased from BASF, Germany. It is a biodegradable and biocompatible polymer which acts as a promoter for the absorption at the gastrointestinal level of the hydrophobic substances [36, 37].
Avicel[R] PH: Microcrystalline Cellulose Avicel (Chemtrec, U.S.A., and Canada), Aerosil[R]: hydrophilic fumed silica with a specific surface area of 200 [m.sup.2] [g.sup.-1] (Degussa, Germany), and Magnesium Stearate (Union Derivan S.A., Spain) have been also used. All used compounds accomplish the quality requirements according with laws in forces. These excipients facilitate the application of the direct compression method, to obtain an optima AMD dispersibility in the powdered mixtures and association of hydrophilic (HP-[beta]-CD, CHT) and hydrophobic substances (AMD).
2.2.1. Preparation of the Inclusion Complexes. To obtain the inclusion complexes of the bioactive compound AMD and the three HP-[beta]-CDs, a 0.179 g amount of HP-[beta]-CD was dissolved in 5 mL of water, and then, AMD was added in a molar ratio of 1: 1. The mixtures have been stirred for 3 days at room temperature, and finally, three opaque solutions have been obtained, the inclusion complexes being partially soluble. The free noncomplexed AMD is separated by centrifugation at 600 rpm for 10 min. at room temperature. Then, the balloon flasks containing solution mixtures have been frozen with liquid nitrogen and dried by lyophilization for 24 h by means of a Labconco FreeZone 2.5 (Kansas City, Mo, SUA) system, and they have been kept in a desiccator. The AMD/HP-[beta]-CD A, AMD/HP-[beta]-CD B, and AMD/HP-[beta]-CD C complexes have been obtained with the three corresponding HP-[beta]-CDs. In the dry state, all samples look like homogeneous white masses with flake-like structures, the compactness decreasing from complex A to complex C (Figure 1).
2.2.2. Characterization of the Inclusion Complexes
(1) Scanning Electronic Microscopy (SEM) Examination. Scanning electronic microscopy (SEM) examination was carried out by using a Quanta 200 scanning electronic microscope (FEI Company, Hillsboro, OR, USA), with an integrated EDX system, and GENESIS XM 2i EDAX (FEI Company, Hillsboro, OR, USA) and with a SUTW detector, at an accelerating voltage of 20 kV and without any further treatments, at different magnifications given on micrographs.
(2) Dynamic Light Scattering (DLS) and Zeta Potential Measurements. The particle size and distribution of the HP-[beta]-CDs and HP-[beta]-CD/AMD complexes were analyzed by the dynamic light scattering (DLS) method using a Zetasizer Nano ZS apparatus (Malvern Instruments, Enigma Business Park, UK) equipped with a He-Ne laser ([lambda] = 633 nm) and operated at a scattering angle of 173[degrees]. The system uses a noninvasive back scatter (NIBS) technology (which reduces the multiple scattering effects) wherein the optic is not in contact with the sample. During determinations, the Mie method is applied over the whole measuring range from 0.6 nm to 6 [micro]m.
The apparent hydrodynamic diameter ([D.sub.H]) of the analyzed samples was determined using the Stokes-Einstein relation:
[D.sub.H] = kT/3[pi][eta]D, (1)
where [D.sub.H] is the hydrodynamic diameter, k is the Boltzmann constant, T is the absolute temperature of 298.15 K, [eta] is the viscosity, and D is the diffusion coefficient.
Zeta potential ([zeta]) was determined on the same Zetasizer Nano ZS device by using the Smoluchowski relationship:
[zeta] = [eta][mu]/[epsilon], (2)
k[alpha][much greater than]1, where [mu] is the electrophoretic mobility, [eta] is viscosity, [epsilon] is dielectric constant, and k and [alpha] are the Debye-Huckel parameter and particle radius, respectively.
From each sample dispersed in ultrapure water at a concentration of 0.06 g [dL.sup.-1], a good dispersion was obtained by simple dissolution, and to improve it, the ultrasonication was applied for 2 h, and then, the samples were analyzed in triplicate at a constant temperature (25[degrees]C) after 2 min of equilibration.
(3) ATR-FTIR Spectroscopy. The ATR-FTIR spectra have been recorded at 2 [cm.sup.-1] resolution with 64 scans by means of a Bruker VERTEX 70 spectrometer (Bruker, Ettlingen, Germany), in the absorbance mode, by the Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy (ATR-FTIR) technique using a Golden Gate system equipped with diamond crystal with an incidence angle of 45[degrees]. Penetration thickness was about 100 microns. Background and sample spectra were recorded in the 600 to 4000 [cm.sup.-1] wavenumber range with a resolution of 2 [cm.sup.-1]. For each sample, the evaluations were made on the average spectrum obtained from three recordings. The processing of spectra was achieved using ORIGIN programs.
(4) [sup.1]H-NMR Spectroscopy. The [sup.1]H-NMR spectra and two-dimensional [sup.1]H-[sup.1]H chemical shift correlation spectra (COSY) were acquired on a Bruker NEO-1 400 MHz spectrometer (Bruker, Germany) equipped with a 5 mm QNP direct detection probe and z-gradients. For the NMR analysis, the compounds (HP-[beta]-CD and complexes) were dissolved in deuterated dimethyl sulfoxide, DMSO-d6, while the amiodarone drug was dissolved in deuterated chloroform, CD[Cl.sub.3]-d, because it is not soluble in DMSO-d6. Deuterated solvents have been purchased from VWR Chemicals, and they have a purity of 99.80% D.
(5) UV-Visible Spectroscopy and Phase Solubility Studies. The direct titration method was performed by using an UV-VIS spectrophotometric method (Cary 60 UV-VIS spectrophotometer; Agilent Technologies) at room temperature. One component of the complex (generally the HP-[beta]-CD) is gradually added at a fixed concentration of the other component of the system (the guest--AMD). Thus, for all experiments, a 0.018 * [10.sup.3] M aqueous solution of AMD was prepared. While the AMD guest was kept at a constant concentration, the HP-[beta]-CD host concentration varied from 0.4 to 8 * [10.sup.3] M. Each solution was kept under agitation for 12 hours. The UV absorption spectrum of each sample was recorded with a 1 mm thick quartz cuvette, and the variation in the absorbance peak of the AMD guest was monitored at 242 nm. Because HP-[beta]-CDs are silent (they do not absorb), the analysis complexity is reduced.
The phase solubility method is widely used to study inclusion complexation. It examines the effect of a solubilizer such as CD or ligand, on the drug which should be solubilized. The phase solubility tests were carried out according to the method described by Higuchi and Connors . Briefly, an excess amount of AMD was added to 10 mL HP-[beta]-CDs in aqueous solutions at concentrations ranging from 0.2 to 10 [M.sup.-3]. The flasks were covered to avoid solvent loss and then stirred for 24 h at room temperature. The resulting dispersions of AMD/HP-[beta]-CD were filtered, and the solution concentration was determined by an UV-VIS spectrophotometric method, the recording being made at 242 nm wavelength characteristic to AMD for which a calibration curve was previously drawn. All the experiments were performed in triplicate. The apparent stability constants ([K.sub.c]) of the complexes were calculated in accordance with Equation (3) from the slope of phase solubility diagrams, where the intercept ([S.sub.0]) is the intrinsic solubility of AMD in water, in the absence of cyclodextrins:
[K.sub.c] = slope/[S.sub.0](1 - slope). (3)
Gibbs free energy of complexation was evaluated using the well-known relation:
[DELTA]G = -RT 1n[K.sub.c], (4)
where R is the gas constant (8.314 J [mol.sup.-1] [K.sup.-1]) and T is absolute temperature in Kelvin (298.15 K).
(6) Differential Scanning Calorimetry (DSC). Differential scanning calorimetry (DSC) was performed for thermal characterization of the samples, using a DSC 823e from Mettler Toledo instrument (Columbus, Ohio, USA) calibrated with indium as the standard. The samples weighing between 2 and 3.5 mg were packed in aluminum pans and placed in the DSC cell. They were heated from ambient temperature to 180[degrees]C at a heating rate of 10[degrees]C [min.sup.-1]. Melting temperature ([T.sub.m]), melting enthalpy ([DELTA]Hm), and degradation temperature ([T.sub.d]) were obtained from first heating run.
(7) Loading Degree of the AMD into HP-[beta]-CD/AMD Inclusion Complexes. The quantitative determination of the loading degree was realized by the HPLC method. In order to determine the loading capacity of the complexes with AMD, the 30 mg complex samples were used. The 3.0-5.0 mg inclusion complex was dissolved in 10 mL methanol, and then, 1.0 mL from the obtained solution was diluted to 10 mL mobile phase resulting in 0.0155 mg AMD [mL.sup.-1]. The free (noncomplexed) AMD is separated by centrifugation at 600 rpm for 10 min at room temperature. The supernatant is collected and analyzed by the HPLC method. The reference sample was methanolic solution of Cordarone (AMD-HCl commercial product) (c = 0.05 mg [mL.sup.-1]).
HPLC was performed by means of a chromatograph of a Thermo Fisher Surveyor type (Thermo Fisher, San Jose, USA) equipped with a UV-VIS detector with multiple Diode Array Detectors and a Thermo Fisher-Hypersil Betasil C18 150 mm * 4.6 mm column (Thermo Fisher, San Jose, USA); the particle size dimension was of 5 [micro]m. The column temperature was kept constant at 45 [+ or -] 0.2[degrees]C. As the mobile phase, a mixture of formic acid 0.5% and methanol in the 25/75 v/v ratio was used at a flow rate of 0.7 mL [min.sup.-1]. The injection volume for each determination was 20 [micro]L. AMD was detected by UV spectrum at its characteristic wavelength at 254 nm. The recorded retention time was 4.51 min. The procedure is described in detail in our previous paper .
(8) In Vitro Dissolution Tests of AMD from Inclusion Complexes. The AMD dissolution profiles have been studied into two dissolution media with different pHs, namely, HCl 0.1 N solution with pH = 1.2 (simulating media for gastric fluids) and phosphate buffer solution with pH = 6.8 (simulating media for intestinal fluids). The experiments were carried out by means of a dissolution test station type II, Hanson SR 8 Plus Series (Hanson Research Co., Chatsworth, USA) provided with two blades. The experiments have been performed according to the requirements described in Romanian , European , and United States Pharmacopoeia (USP)  with specifications for liquid and solid pharmaceutical formulations. The following protocol was followed: 100 mg of pure AMD quantity and equivalent amount of complexes which contain also 100 mg AMD evaluated according to loading degree were added into 500 mL of each above-mentioned dissolution medium. Temperature was kept constant at 37 [+ or -] 0.5[degrees]C, and the stirring speed was of 60 rpm. Aliquots (2 mL) of the dissolution medium were withdrawn, examined, and replaced with fresh dissolution media at predetermined time intervals of 5 minutes in the first 30 minutes, then at intervals of 10 minutes for the next 2 h and then at intervals of 30 minutes for the next 2 h. The AMD in prevailed solution was quantitatively determined by the above-described and validated HPLC method . Tests have been made in triplicate, and the average value was given.
(9) Kinetic Release of AMD from Inclusion Complexes. The release kinetic parameters were calculated using the equation proposed by Korsmeyer et al. :
[mathematical expression not reproducible], (5)
where [M.sub.t]/[M.sub.[infinity]] represents the fraction of the drug released at time t, [k.sub.r] is a constant incorporating characteristics of the macromolecular matrix, and [n.sub.r] is the diffusion exponent, which is indicative for the release mechanism. In the above equations, a value of [n.sub.r] = 0.5 or smaller indicates a Fickian diffusion mechanism of the drug from the matrix--as for step 1 at both pHs and step 2 at pH 6.8; a value 0.5 < [n.sub.r] <1 indicates an anomalous or non-Fickian behavior. This is characteristic to step 1 and step 2 at pH 6.8. When [n.sub.r] = 1, a case II transport mechanism is involved while [n.sub.r] > 1 indicates a special case II transport mechanism of step 3 at pH 1.2 (see below).
2.2.3. Matrix Tablet Formulation Preparation. Two formulations based on KOL and CHT as matrix constituents have been prepared, one containing only pure AMD as the active compound (designed as F) and another containing AMD/ HP-[beta]-CD B complex (designed as Fc). The AMD/HP-[beta]-CD B inclusion complex was selected for the oral therapeutic system preparation because of its good physicochemical and pharmacotechnical properties in comparison with the other two AMD/HP-[beta]-CD A and AMD/HP-[beta]-CD C complexes as it was demonstrated by their characterization (see below). The purpose of this strategy was to evaluate the influence of the therapeutic system type on the oral biodisponibility of AMD and to analyze the influence of AMD complexation with HP-[beta]-CD B on this pharmacokinetic parameter. In Table 2 are presented the compositions of the two oral formulations, where KOL and CHT and other specific auxiliary excipients were included [37, 44, 45].
An Erweka AR 403 device (Erweka GmbH, Heusenstamm, Germany) was used for these preparations in the following conditions: rotation speed 400 rpm for 5 min; after that, the mixtures were sieved with an electromagnetic sieve EM-8 (Erweka GmbH, Heusenstamm, Germany). Direct compression of the mixtures was done with a Korsch EK0 single-punch station press (Korsch AG, Berlin, Germany) (punch diameter 9 mm, compression force 8-10 kN). The matrix tablets with an average diameter of 12 mm and thickness of 4.6 mm have been obtained (Table 2).
(1) Pharmacotechnical Characterization of the Matrix Tablets Containing AMD and AMD/HP-[beta]-CD B. The evaluation of the pharmacotechnical characteristics (diameter, thickness, and average mass) of the tablets was performed according to the 104 Romanian Pharmacopoeia and 8th European Pharmacopoeia [40, 41]. Weighing was made by means of an electronic balance Radwag WPE 60, on 20 tablets for mass and dose uniformity determination. Mechanical resistance was performed on 10 tablets on a Schleuniger Pharmatron tablet hardness tester 8M (Sotax AG, Aesch, Switzerland) and the friability on 20 tablets on Schleuniger Pharmatron FT II friability tester (Sotax AG, Aesch, Switzerland), at 100 rpm for 4 min. All pharmacotechnical characteristics have been performed comparatively for the two studied formulations, F and Fc. The dose uniformity was established using a validated HPLC procedure through the quantitative determination of AMD from a fine powder obtained by the trituration and the homogenization of 20 tablets with known average masses. The SD should be of [+ or -] 5%  in all cases. According to pharmacotechnical specifications for the preparation of modified-release tablets, the release profiles of the active substance from such type of tablets must be analyzed by determining the difference factor f1 and the similarity factor f2 between two or more formulations .
Hydration capacity or swelling degree was determined by using a dissolution test station type II, Hanson SR 8 Plus Series (Hanson Research Co., Chatsworth, USA). Matrix tablets have been introduced in 1000 mL distilled water at 37[degrees]C at 60 rpm. At the predetermined time intervals (1 h), samples were prevailed from hydration medium; the water excess on the surface was removed by wiping with filter paper, and then, they have been weighed. The swelling degree was evaluated using relation:
SD = [[W.sub.t] - [W.sub.o]/[W.sub.o]]100, (6)
where SD is the swelling degree, [W.sub.t] is mass of the sample at time t, and [W.sub.o] is dry mass of the tablet.
(2) In Vitro Release Tests of AMD from Matrix Tablets. In vitro release of AMD from the matrix tablets F and Fc have been determined into simulating gastric fluid with pH = 1.2 and into simulating intestinal fluid with pH = 6.8 until the release amount reached equilibrium (maximum of 10 hours). The method uses the same equipment and working conditions as described above at In Vitro Dissolution Tests of AMD from Inclusion Complexes. Tests have been made in triplicate, and the average value was given.
(3) In Vivo Single-Dose Pharmacokinetic Study. The single-dose pharmacokinetics of the new formulation Fc, which contains AMD/HP-[beta]-CD B complex, was investigated comparatively with formulation F1 containing pure AMD.
Ethics Statement. The experiment was approved by the Ethics Committee of "Grigore T. Popa" University of Medicine and Pharmacy of Iasi, Romania (No. 23983/2014) and was carried out in accordance with the European regulations concerning the studies with animals. The study was carried out on male Wistar rats (average weight of 250-300 g) provided by the Cantacuzino Institute--Bucharest, Romania.
Experimental Design and Sampling. The rats were housed in polypropylene cages with controlled environmental conditions of temperature (21 [+ or -] 2[degrees]C) and humidity (50-70%) and successive light and dark of 12 h cycles and on ad libitum access to food and water. After three days of acclimation, on the evening of the day before administration of the substances and until 4 h post administration, no food was administered to all rats, but they had free access to drinking water. A temporary cannula with a 24 G catheter was placed in the lateral tail vein for blood collection as "free flowing," after warming the animals at 37[degrees]C for 10 minutes in order to allow tail vein vasodilation. The substances were administered by oral gavage as freshly prepared solutions, as a single oral dose of 100 mg substance/kg body in a volume of 0.2 mL [(100 g).sup.-1] body. Each animal was weighed before the administration of the substances. Rats were randomly assigned into three groups of six animals each, according to the following protocol: Group 1 (control AMD group) received free drug suspension of AMD in aqueous polysorbate 80 (c = 0.1%w/v), Group 2 (F group) received F formulation, and Group 3 (Fc group) received Fc formulation. Approximately 0.3 mL of blood was collected into heparinized tubes at 0 (predose), 0.25, 0.5, 1, 2, 3, 4, 5, 6, 8, 10, 12, 24, and 48 h postdose. A heparin flush of 0.1 mL was used after placement and between blood samples to prevent clotting. Blood samples were centrifuged at 4000 rpm for 10 min; then, plasma was stored at -20[degrees]C until analysis. Plasma samples were analyzed using the HPLC method. The lower limit of quantification for AMD was established at 5.83 [micro]g [mL.sup.-1].
Pharmacokinetics and Statistical Analysis. The plasma pharmacokinetic parameters directly determined by inspection of the individual drug plasma concentration versus time curves included the observed maximum plasma concentration ([C.sub.max]) and the observed time ([t.sub.max]) to reach maximum concentration. The area under the curve from 0 h to last determined experimental data ([AUC.sub.0-t]) was estimated by the linear trapezoidal rule calculated from the individual AUCs from the plasma concentrations versus time curves. Noncompartmental pharmacokinetic analysis was performed using TopFit 2.0 Pharmacokinetic Software (Thomae GmbH, Germany) to determine other main pharmacokinetic parameters: area under the plasma concentration time curve from time zero to infinity ([AUC.sub.0-[infinity]]), elimination rate constant ([k.sub.e]), and half-life ([t.sub.1/2]). The elimination rate constant ([k.sub.e]) for each rat was estimated from the slope of the regression line of plasma concentrations versus time curves by the least squares regression analysis, and the apparent elimination half-life ([t.sub.1/2]) was calculated from the equation [t.sub.1/2] = ln (2)/[k.sub.e]. Systemic relative bioavailability ([F.sub.rel]) was calculated by the following equation:
[F.sub.rel] = [AUC.sub.test]/[AUC.sub.reference], (7)
where [AUC.sub.reference] is the AUC of free AMD and [AUC.sub.test] is the AUC of the studied formulations. Results of pharmacokinetic studies were expressed as the mean [+ or -] standard deviation (SD). Differences in the pharmacokinetic parameters were analyzed with the one-way analysis of variance (ANOVA) test using SigmaPlot 11 (SxST.it, Italy) software, and a p value of <0.05 was considered significant.
3. Results and Discussion
3.1. Characterization of the 2-Hydroxypropyl-beta-cyclodextrins and Corresponding Inclusion Complexes
3.1.1. Scanning Electron Microscopy (SEM) Results. Given in Figure 2 are the SEM images of AMD, 2-hydroxypropyl-beta-cyclodextrins, and corresponding inclusion complexes.
Powder of amiodarone exhibits cylindrical microcrystals of various sizes with tendency of aggregation. 2-HP-[beta]-CDs in the dry state look as spherical particles of various dimensions. The HP-[beta]-CD A particles show a smooth surface, while the other two HP-[beta]-CD B and HP-[beta]-CD C show also spherical shapes, but on their surfaces, some porosities are evident, probably because of the most stable configuration at the high substitution degree. The morphology of the inclusion complexes is homogeneous, totally different in respect of those of the components (AMD and HP-[beta]-CDs), and it is specific for each complex. Generally, they look as fibrous materials with a particular aspect and integrity for each of them depending on both substitution degree and molecular weight. The SEM image of the AMD/HP-[beta]-CD B complex is very different in respect of those of other two complexes (AMD/HP-[beta]-CD A and AMD/HP-[beta]-CD C). This particular morphology and particle shape are indicative of the formation of the new solid phase.
The histograms in Figure 3 look as the curves with one peak for HP-[beta]-CD A and HP-[beta]-CD C samples. From these curves, the average diameters of the particles were determined at 20 [micro]m and 9 [micro]m, respectively. The HP-[beta]-CD B showed a bimodal histogram with two characteristic particle diameters of 20 [micro]m and 70 [micro]m; therefore, in this case, the particle distribution is particular.
3.1.2. Dynamic Light Scattering (DLS) and Zeta Potential Measurements. The DLS technique was employed to monitor the hydrodynamic size and colloidal stability of the HP-[beta]-CDs and inclusion complexes dispersed in water. The obtained results are shown in Figure 4 and Table 3.
The size distribution profiles of HP-[beta]-CD A and HP-[beta]-CD C resemble each other, showing one low-intensity peak at around 3 nm and a second main one which ranges from 140 nm to 165 nm--Figures 4(a) and 4(b). Three different particle size populations coexist in the HP-[beta]-CD B sample. The first one is the size population that can be observed as the first part of the curve with a diameter of ~3 nm which is associated to the formation of small assemblies containing 2 or 3 HP-[beta]-CD units . A second size population appears at around 77 nm and a third one with the highest DH at around 530 nm. These results can be explained by the fact that HP-[beta]-CDs are known to self-assemble in aqueous solutions to form clusters with intermolecular linkage attributed to the OH groups located at the rims of the donut-shaped molecules . Similar results have been obtained by Gonzalez-Gaitano et al.  who also used dynamic light scattering technology to study the association of CDs. They showed the formation of aggregates in aqueous solutions (with a DH ranging from 124 to 172 nm) together with nonassociated molecules.
The inclusion complexes displayed the different size distribution curves and histograms, being much homogeneous--Figures 4(a) and 4(b). The [D.sub.H] range from 189 to 295 nm and lower polydispersity index values when compared to those of the pure HP-[beta]-CDs. The PDI is an indicator that measures the heterogeneity of the materials in aqueous solution, a smaller value indicating a narrower size distribution which is most likely related to a lesser tendency of the complexes to form aggregates.
The stability of the formed complexes can also be estimated by zeta potential assessment. The results (Table 3) demonstrate a stabilization of the complexes, AMD/HP-[beta]-CD B showing the highest zeta potential value (17.3 mV) which suggests that this is the most stable formulation, followed by AMD/HP-[beta]-CD A (15.7 mV) and AMD/HP-[beta]-CD C (10.9 mV). The DLS results are in a good accordance with those obtained by SEM examination.
3.1.3. ATR-FTIR Results. The ATR-FTIR spectra of the three HP-[beta]-CDs are similar (Figures 5(a)-5(c)) and are in agreement with those reported in other papers [14, 50-55]. A characteristic absorption band of [alpha]-type glycosidic bond was found at 850 [cm.sup.-1], which indicates that HP-[beta]-CDs were formed by glucopyranose units through [alpha]-1,4-glycosidic bond .
The main bands corresponding to AMD are the following: 2964 and 2934 [cm.sup.-1] assigned to a CH aliphatic stretching, 2457 [cm.sup.-1] corresponding to tertiary amine stretch, and in the region of 1285 [cm.sup.-1] the band referred to as ketone C=O group bending . The three complexes (AMD/HP-[beta]-CDs A, B, and C) show some differences in respect of spectra of their components (HP-[beta]-CD and AMD), which were evidenced in different spectral regions (Figure 5).
The 1022 [cm.sup.-1] and 755 [cm.sup.-1] bands from HP-[beta]-CD had shifted to 1031 [cm.sup.-1] and 750 [cm.sup.-1] in case of the inclusion complexes formed with AMD. The 2457 [cm.sup.-1] band in the AMD spectrum is much wide in the spectra of the inclusion complexes because of the combination of the stretching of the C-H and C-C bonds. The intensity of the group of bands placed between 1600 [cm.sup.-1] and 950 [cm.sup.-1] characteristic to the amiodarone decreases in the spectra of the inclusion complexes. The most evident decrease in intensity was observed in the case of the band from 1629 [cm.sup.-1]. Changes of the bands in spectra of the components of the inclusion complexes such as shifts and increase or decrease in intensity were reported by other authors  which were explained by the insertion of the benzene part ring into the electron-rich cavity of [beta]-cyclodextrin [16, 53] and due to the changes in the microenvironment which lead to the formation of hydrogen bonds and the presence of van der Waals forces during their interaction to form the inclusion complexes [54, 55].
As it can be seen in Figure 6(a) (3600-2700 [cm.sup.-1] region), the bands in the spectrum of the inclusion complex AMD/HP-[beta]-CD C are much broader than those of the other two complexes, and also, some differences appear in the position of the OH and CH stretching, namely, 3373 [cm.sup.-1] and 2924 [cm.sup.-1].
The shapes of the spectra in the 1700-1200 [cm.sup.-1] region are specific to each complex because of the variation in band position and in intensity, as observed in Figures 6(b) and 6(c).
Thus, differences between the three inclusion complexes of some characteristic band positions were found as complex A: 1657 [cm.sup.-1],1563 [cm.sup.-1], and 1555 [cm.sup.-1]; complex B: 1653 [cm.sup.-1], 1558 [cm.sup.-1]; and complex C: 1655 [cm.sup.-1],1646 [cm.sup.-1] which can be assigned to OH bending and amide II. The band position is characteristic to each complex which means that also some interactions with substituent characterize these complexes due to the appearance of host-guest interactions.
3.1.4. [sup.1]H-NMR Results. NMR study is the most important tool which ascertains the inclusion phenomena of the guest drug molecule inside the host CD molecule. The raw materials and the obtained complexes were characterized by [sup.1]H-NMR--Figures 7(a) and 7(b).
Comparing the three superposed spectra, in the complex spectrum, the signals for the raw materials can be identified and the appearance of a new signal, as a singlet at 10.44 ppm--Figure 7.
In the 2D NMR spectrum (Figure 8), the couplings between HP-[beta]-CD signals of 3.38 and 3.68 ppm and a new signal at 10.44 ppm in the obtained complex can be observed. This signal indicates a direct H-H connection between components of the complexes  and also suggested that the lipophilic aromatic ring of the AMD entered into the cavity of HP-[beta]-CD from the wider side .
3.1.5. UV-Visible Spectroscopy and Phase Solubility Study Results. The formation of inclusion complexes of AMD with the three types of HP-[beta]-CDs in aqueous solutions was followed by the measurements of the absorbance at 242 nm which is characteristic to AMD as a function of HP-[beta]-CD concentration--Figure 9. The results show that the absorbance at 242 nm increases with the concentration of HP-[beta]-CD which is due to the increase in AMD solubility (increased AMD solution concentration) as it is being included in the HP-[beta]-CD cavity. It was clear that the solubility of AMD/HP-[beta]-CD was much higher than that of pure AMD. These results indicated that HP-[beta]-CD formed inclusion complexes with AMD, which improved the aqueous solubility of AMD. It seems that the shapes of the UV-VIS spectra of the complex B are a little different from the other two; the main absorbance band (242 nm) is narrower, and values of maximum absorbance are smaller at each concentration.
The physicochemical properties of CDs, including their complexation ability, are affected by the type, number, and position of the substituents linked on the CD molecule . The degree of substitution of the HP-[beta]-CD, the substituent position, and purity also influence these properties. During the substitution reaction, new reactive sites are generated in HP-[beta]-CD derivatives. Derivatives with a low degree of substitution showed the best complexation properties with low surface activities. As the substitution increased, the steric hindrances weakened the binding. All these effects are also dependent upon the particular guest, as the drug nature. In many cases, the effect of the degree of substitution differences in the binding capacity was small .
As observed in Figure 10, the AMD guest solubility linearly increased with increasing concentrations of HP-[beta]-CDs over the concentration range evaluated, indicating a 1 : 1 molecular complex formation between AMD and the three HP-[beta]-CDs. For further experimental analyses, the molar ratio (1:1, AMD:HP-[beta]-CD) was chosen to prepare the inclusion complexes as this ratio was also established in our previous study . This is a phase AL type diagram which indicates the formation of water soluble inclusion complexes with linear increases of drug solubility as a function of HP-[beta]-CD concentration. The AMD precipitation does not occur during dilution. The formation of the inclusion complexes was demonstrated by the increase of the solubility in water (to 0.0879 g [L.sup.-1] of about 2.2 times) of AMD in the presence of HP-[beta]-CD in comparison with the free AMD (of 0.04 g [L.sup.-1]).
The stability constants ([K.sub.c]) of AMD/HP-[beta]-CD A, AMD/HP-[beta]-CD B, and AMD/HP-[beta]-CD C complexes (1:1) were evaluated as 1218, 1462, and 1736 [M.sup.-1], respectively, from the linear plot of the phase solubility diagram (Figure 10). The corresponding free energy changes (AG) are -17.60 kJ [mol.sup.-1], -18.06kJ [mol.sup.-1], and -18.48 kJ [mol.sup.-1], respectively. These values are in the same limits with those found for other inclusion complexes of HP-[beta]-CD with various drugs .
For the three HP-[beta]-CDs, the stability constants and negative [DELTA]G increased with the increase in the molecular weight (degree of substitution) of the HP-[beta]-CD and the decrease of the particle size, behavior which was also observed by Liu et al. . This is probably due to the enhancement of their binding capacity with AMD as a result of the increased stability with increasing substitution by hydroxypropyl groups. Yuan et al.  found that increasing substitution on the secondary hydroxyl groups of CDs could extend their cavity and thus improve the complex forming ability, which agreed with our results. The AMD/HP-[beta]-CD B and AMD/HP-[beta]-CD C complex seems to be more stable.
Taking into consideration the interactions between the inclusion complex partners evidenced in spectral study, the following chemical structure may be assigned to the complex (Figure 11).
Amiodarone as an iodine-rich benzofuran derivative is loaded in cavity of HP-[beta]-CDs; its functional groups interact both with the base molecule (CD) and substituent (HP) by their OH groups.
3.1.6. Thermal Characterization. The DSC curves of the three complexes and their components are given in Figure 12. AMD has a particular curve, characteristic to the crystalline compounds with a sharp melting peak with a melting temperature ([T.sub.m]) of 163.8[degrees]C and a melting heat ([DELTA][H.sub.m]) of 112.0 J [g.sup.-1] (Table 4).
The thermal characteristics of the HP-[beta]-CD A are different from those of the other two HP-[beta]-CDs. Both [T.sub.m] and A [H.sub.m] take higher values of 89.3[degrees]C and 103 J [g.sup.-1] while the values for HP-[beta]-CD B and HP-[beta]-CD C cyclodextrins are of [T.sub.m] of 68[degrees]C and [DELTA][H.sub.m] of 33.8 and 40.9 J [g.sup.-1], respectively (Table 4). The complexes have two melting peaks corresponding to both components, but they are shifted at lower temperature. The melting peak of AMD decreases from 163.8[degrees]C to 153-154[degrees]C, while those of HP-[beta]-CDs A, B, and C are placed at 65.5[degrees]C, 70.2[degrees]C, and 71.4[degrees]C, respectively. The melting heat of the first peak increases in the order complex A < complex B < complex C and decreases in the same order in the case of the second melting peak. This means an important decrease of the crystallinity of AMD by complexation which explains its higher solubility. This also can be explained by the particularities of each complex which determine finally their differences in solubility, loading, and release behavior of AMD.
3.1.7. Loading Degree of AMD in Inclusion Complexes. In Figure 13 are presented the HPLC chromatograms of the three inclusion complexes comparatively with AMD. Comparing the peak areas, it can easily be observed that the AMD quantity included in complexes varies in the order: AMD/HP-[beta]-CD B > AMD/HP-[beta] A > AMD/HP-[beta] C. The complex C shows the smallest area under the curve as it includes the smallest AMD amount.
The sample concentration Cp in mg [mL.sup.-1] of AMD was obtained from HPLC results using the following equation:
Cp = (Peak Area + [39.693/54377]) mg [mL.sup.-1], (8)
Complexation efficiency (CE) or loading efficacy of the complexes with AMD was evaluated as the ratio of AMD content in complexes (AMD/HP-[beta]-CD A, AMD/HP-[beta]-CD B, and AMD/HP-[beta]-CD C), determined by HPLC, to the theoretical AMD content, and was expressed in percentages by using the following relation:
%CE = [(AMD total - AMD free/AMD total)] * 100. (9)
The obtained results are summarized in Table 5.
The complexation efficiency is very high of 96.09-99.49%, while the free AMD (not included in complex) is low varying in the 0.5%-3.91% interval. The values of the relative standard deviation (RSD) indicate that there are no significant modifications by the sample processing. Average recovery was 99.38%, while recovery at the three complexes loadings is in the limits of [+ or -] 5% [39, 61].
3.1.8. In Vitro Release Test. Release profiles of neat AMD and those incorporated into inclusion complexes at two pHs, obtained according to the above-described protocol, are given in Figure 14.
The dependence on pH of the dissolution/release behavior was also established in our previous paper  for the AMD/HP-[beta]-CD system. An increase in pH results in the increase of the released AMD quantity from complexes especially in the first 100 min, but not in increased solubility of pure AMD (Table 5 and Figure 14). This can be explained due to the formation of partially insoluble complex between drug and anions dissolved in buffer solution [62-64].
The release of AMD from complexes starts with a burst effect which occurs in the first 6-7 minutes, where the AMD released concentration increased very fast. The released AMD amount at the end of this stage was AMD/HP-[beta]-CDB (7.7 %) > AMD/HP-[beta]-CD A (5.99%) > AMD/HP-[beta]-CD C (4.95 %) > free AMD (3.8%) for pH 1.2, while at pH 6.8, the order is kept but the released amount is higher as AMD/HP-[beta]-CD B (28.2%) > AMD/HP-[beta]-CD A (25.8%) > AMD/HP-[beta]CD C (24.4%) > free AMD (4.94%) (Table 6).
After 120 minutes, at pH 1.2, the released AMD varies in close limits for all complexes being of AMD/HP-[beta]-CD B (19.58%) > AMD/HP-[beta]-CD A (18.90%) > AMD/HP-[beta]-CD C (17.02%) comparative with values of only 3.8% for free AMD. This means an increase of about 5 times of drug solubility. The dissolution of free AMD of 4.25% remains almost constant for the entire studied time period while the AMD release from inclusion complexes occurred in three stages, and at the equilibrium (up to 300 min), the total amount of the released AMD was AMD/HP-[beta]-CD B (83.7%) > AMD/ HP-[beta]-CD A (78.5%) > AMD/HP-[beta]-CD C (72.4%).
At pH 6.8, the release behavior is different in respect of that at pH 1.2. The release takes place very fast in the first 2-3 min reaching 25-30% that means a significant burst effect, followed by a step with a very slow release rate. The three steps are also evident: the first two occurs until 120 min with a ADM released of about 28-35.5%, and the last one took place in 120-300 min period, the released AMD reaching about 88% a little higher than that at pH 1.2. The AMD released rate from complexes is higher at pH 6.8 as a consequence of the fact that this pH value is close of p[K.sub.a] = 6.64 characteristic to amiodarone. The release rate from inclusion complexes increases as a consequence of the crystallinity decrease of the drug by complexation according to DSC results, which determines an increase of the solubility. At the end of the experiment, the order of the AMD released amount was AMD/HP-[beta]-CD B (89.2%) > AMD/HP-[beta]-CD A (87.5%) > AMD/HP-[beta]-CDC (85.5%) in all cases the percentage released being higher than that at pH 1.2. In respect of the free AMD, an increase of 22% of AMD is released from inclusion complexes with the highest amount from the AMD/HP-[beta]-CD B complex.
In Table 7 are reported the released kinetic parameters calculated using Equation (5) which was applied to the initial (0-50 min), second (60-120 min), and third (120-300 min) steps of drug release.
At pH 1.2, a shift of the drug transport mechanism from pseudo-Fickian to non-Fickian behavior was observed between the first and the second kinetic release steps, where the values of the release exponent were under 0.5 ([n.sub.r] = 0.18-0.28) in the first kinetic release step and ranged between 0.5 and 1 ([n.sub.r] = 0.68 - 0.95) in the second kinetic release step (Table 7). At pH 6.8, only one type of drug transport mechanism was reported, namely, a pseudo-Fickian diffusion mechanism. A case II of the transport mechanism was observed in the third kinetic release step at pH 6.8 ([n.sub.r] = 0.93 - 1.00) and a special case II transport mechanism at pH 1.2 ([n.sub.r] = 1.53 - 1.60). Both drug release media of pH 1.2 and pH 6.8 caused the most increased values of the release exponent ([n.sub.r]) in case of the AMD/HP-[beta]-CD B inclusion complex.
The kinetic constant [k.sub.r] values, also reported in Table 7, are higher in the case of the drug release profile of inclusion AMD/HP-[beta]-CD B complex, and this fact suggests that the drug diffusion degree is much increased in comparison with the inclusion AMD/HP-[beta]-CD A complex and AMD/HP-[beta]-CD C complex. This behavior was observed in both simulating media of pH 1.2 and 6.8.
The obtained values for AMD released from the three complexes after 300 minutes support the hypothesis that the AMD incorporation into inclusion complexes is a good way to realize pharmaceutical formulations with oral delivery and controlled release. If at the pH 1.2 the solubility is still reduced, it is optimized at pH 6.8 for such way of administration of AMD which is more convenient for patients. The prolonged release in the 6-8 h time interval is in accordance with optima pharmaceutical requirements for such formulations, to avoid the risk of AMD accumulation in the organism which should lead to the major toxic phenomena [65, 66].
3.2. Characterization of the Matrix Tablets of AMD and Corresponding Inclusion Complex
3.2.1. Pharmacotechnical Characterization. Pharmacotechnical characteristics given in Table 8 demonstrated that incorporation of the AMD as the inclusion complex with HP-[beta]-CD especially AMD/HP-[beta]-CD B complex in matrix tablets based on KOL and CHT does not change the pharmacotechnical properties of the tablets. Only one relevant modification was observed in the values of the hydration degree which was found superior to Fc (124% in respect of 115% for F). This behavior is expected to positively influence the release profile and bioavailability of the active principle.
The mass uniformity shows a deviation of [+ or -] 5% in respect of the mass of common tablets of [greater than or equal to]250 mg while the dose uniformity values correspond to the individual content of active substance of each unit which should be of 85% to 115% in respect of average content. All obtained values of the pharmacotechnical characteristics are in the limits of the specifications reported in literature . Friability and mechanical strength values indicate that by complexation, the AMD properties as flowability and compressibility are not changed, because between values, significant differences are not registered and these values are very close to those of the tablets with optima pharmacotechnical characteristics, namely, approximately 1% for friability and 90-100 N for mechanical strength. These results proved that the pharmacotechnical characteristics of the matrix tablets of AMD/HP-[beta]-CD inclusion complexes are very good and the selected formulations included necessary excipients to obtain optima tablet formulations .
3.2.2. In Vivo Single-Dose Pharmacokinetic Study from the Matrix Tablets. Pharmacokinetic behavior as the mean plasma concentration vs. time curves of formulation Fc comparative with control samples of formulation F and of pure AMD suspension (corresponding to free AMD), in male Wistar rats after single oral administration of 100 mg [kg.sup.-1], is plotted in Figure 15.
Individual values of [C.sub.max], [t.sub.max], and [AUC.sub.0-t] of formulation Fc comparative with control samples of formulation F and of free AMD suspension from experimental data are presented in Table 9.
The areas under curves from 0 h to the last determined experimental data ([AUC.sub.0-t]) shown in Table 10 are 3143 [+ or -] 934 ng [mL.sup.-1] [h.sup.-1] for pure AMD suspension, 3591 [+ or -] 1052 ng [mL.sup.-1] [h.sup.-1] for formulation F, and 16184 [+ or -] 2924 [mL.sup.-1] [h.sup.-1] for Fc, a value of 5 times higher for Fc in respect of pure AMD suspension and a value of approximately 4.5 times higher for Fc in respect of F.
A high value of variability (CV %) of observed pharmacokinetic parameters is in accordance with the high interindividual variability of AMD.
Comparative pharmacokinetic parameters of formulation Fc in respect with control samples of formulation F and of free AMD are listed in Table 10.
Noncompartmental pharmacokinetic analysis was performed using TopFit 2.0 Pharmacokinetic Software (Thomae GmbH, Germany) to determine other main pharmacokinetic parameters: area under the plasma concentration time curve from time zero to infinity ([AUC.sub.0-[infinity]]), elimination rate constant ([k.sub.e]), and half-life ([t.sub.1/2]). The elimination rate constant ([k.sub.e]) describes the rate at which a drug is removed from the body of each rat which was estimated from the slope of the regression line of plasma concentrations versus time curves by the least squares regression analysis, and the apparent elimination half-life ([t.sub.1/2]) was calculated from the equation:
[t.sub.1/2] = ln (2)/[k.sub.e]. (10)
The AMD was better absorbed at the tested dose from the formulation Fc and poorly absorbed from the pure AMD suspension and from the F1 formulation. It was noted that the [C.sub.max] obtained following the oral administration of a single dose of formulation Fc in rats was 1.5 and 3.8 times higher in comparison to the [C.sub.max] obtained following formulation F and pure AMD suspension oral administration, respectively. The values of the AMD plasma concentrations released from Fc formulation independent of time belong to <2000 ng[mL.sup.-1], the same therapeutic interval as that found by other authors for humans [68, 69], and these values are similar with those reported in other studies concerning the pharmacokinetic studies using rats [70-72]. After 24 h from administration, the values of the plasma concentration released from Fc are also higher than those of the two control samples. This means a better absorption of AMD and a prolonged release from this new Fc formulation.
Further, the relative bioavailability of AMD following oral administration of formulation Fc was 4.5-fold and 5.1-fold higher in Wistar rats than that of formulation F and pure AMD suspension, respectively. This is an incontestable proof in favor of practical use of such matrix tablets. Relative bioavailability of F formulation was about 1.1 times higher compared to pure AMD suspension and also could be explained by the effect of complexation of AMD in the formulation Fc, which ultimately enhanced the digestive absorption.
A new modified-release formulation not only alters the release of a drug but also influence the release and dissolution rate in the different digestive sites because of different rates of absorption, leading to multiple peaking phenomena [73-75].
The double peak in the pharmacokinetic profile of the new formulation Fc (Figure 12), compared with the references pure AMD suspension and F formulation, may indicate differential absorption rates at the digestive level. These data are also in accordance with those found by in vitro study which evidences three steps of AMD release from the inclusion complexes, which could be explained both by the free AMD presence for the first stage of release as burst effect and by different kinds of bonds in complexes because of substituents and their distribution and [beta]-CD chains. As compared to the pure AMD suspension reference, the new formulation Fc presented a 1.5-hour delay in the starting of each peak in plasma (see insert), and the amount of AMD absorbed from this new modified-release formulation is higher. The first peak of plasma concentration of AMD in the pure AMD suspension is 136.67 [+ or -] 52.0 ng [mL.sup.-1] and occurs at 4 hours after administration, while the first peak of plasma concentration of AMD in the formulation F is 186.0 [+ or -] 56.4 ng [mL.sup.-1] and occurs at 3 hours after administration while in the formulation Fc, the concentration was 495.67 [+ or -] 95.4 ng [mL.sup.-1] and occurs at 4 hours after administration. The second peak of plasma concentration of AMD in the pure AMD suspension is 231.33 [+ or -] 40.4 ng [mL.sup.-1] and occurs at 4 hours after administration, while the first peak of plasma concentration of AMD in the formulation F is 188.0 [+ or -] 98.0 ng [mL.sup.-1] and occurs at 5 hours after administration and in the formulation Fc is 625.33 [+ or -] 95.4 ng [mL.sup.-1] and occurs at 8 hours after administration.
Following oral administration, the Fc formulation showed statistical improvement in the pharmacokinetics of AMD formulation as determined by AUC (increased by approximately 5 times, p < 0.05) and [C.sub.max] (increased by 3.6 times, p < 0.05). The [t.sub.max] time needed to obtain the [C.sub.max] of AMD was delayed for Fc formulation ([t.sub.max] = 6.3 [+ or -] 2.0 h), a value higher than those of reference compounds pure AMD suspension ([t.sub.max] = 5.5 [+ or -] 1.9 h) and formulation F ([t.sub.max] = 5.0 [+ or -] 2.2 h), respectively. Therefore, the release of active substance from Fc formulation occurred later, but with better bioavailability than those of the reference samples.
Matrix formulation Fc containing complexed AMD enhanced the bioavailability of insoluble hydrophobic AMD by increasing the drug solubility, dissolution, and/or drug permeability, making it available at the surface of the biological barrier, from where it partitions into the membrane without disrupting the lipid layers of the barrier. The increased drug efficacy and potency (i.e., reduction of the dose required for optimum therapeutic activity), caused by HP-[beta]-CD presence increased drug solubility, may reduce drug toxicity by making the drug effective at lower doses. The inclusion complex of AMD with HP-[beta]-CD might reduce the side effects by limiting the interaction and might show better tolerance with lower incidence and severity of side effects compared with the free drug. The HP-[beta]-CD complexation provides better absorption of drugs with poor and erratic absorption and might enhance the drug activity on oral administration. HP-[beta]-CDs were shown a better oral safety profile. The inclusion of HP-[beta]-CD/AMD complexes exhibited a much higher aqueous solubility and allows also a parenteral administration of various drugs with no significant toxicity problems and hence are more often used in parenteral formulations [76-78].
AMD/HP-[beta]-CD B complex as such and as Fc formulation exhibited reduced drug tissue irritation and precipitation tendency because their pH values were significantly closer to the physiological value (pH 7.4). AMD/HP-[beta]-CD complexes are useful as slow-release carriers, in prolonged release formulations of water insoluble AMD drug.
In order to develop oral AMD pharmaceutical formulations with prolonged release, three inclusion complexes with different kinds of HP-[beta]-CDs have been prepared. The HP-[beta]-CDs differ by their average molecular weight and substitution degrees. Their structural, morphological, and thermal characterization evidenced differences in particle size, types of bonding, and crystallinity on the HP-[beta]-CD type. A substitution degree of 0.8 assured the most suitable characteristics for complex with amiodarone as stability and loading and release behavior. All studied complexes led to the increased AMD solubility and also determined different behaviors in AMD dissolution/release proved both by in vitro and by in vivo tests performed into media with two different pHs.
It has been established that in all complexes the ratio between the two components is roughly 1 : 1. Since the loading efficacy with AMD of the three complexes varied from 96.09 to 99.49% in the order: AMD/HP-[beta]-CD B > AMD/ HP-[beta]-CD A > AMD/HP-[beta]-CD C; the highest efficacy was found for the AMD/HP-[beta]-CD B complex containing 2-HP-[beta]-CD with the degree of substitution 0.8 and molecular weight 1460 Da. The AMD release from inclusion complexes occurred at the higher amount up to 22% times more than that from free AMD.
Matrix tablets containing Kollidon[R] and chitosan as available and cheap excipients have been obtained by direct compression using high amounts of complex (200 mg/matrix tablet). Such matrix tablets showed a prolonged and sustain modified release and a bioavailability of 5 times higher than those of conventional tablets. This offers a prolonged oral delivery at the plasmatic concentrations belonging to the efficient therapeutic concentrations.
The formulations of AMD/HP-[beta]-CD inclusion complexes both from powdered form and from matrix tablets showed superior pharmacokinetic performance by improving loading and release properties of the insoluble AMD drug. In vitro kinetic study revealed a complex mechanism of release occurring in three steps: the first one being attributed to a burst effect and the other two to different bonding in inclusion complexes. The in vivo test on matrix tablets containing Kollidon[R] and chitosan, similarly, shows significant difference between the plasma drug levels over time between the free drugs as pure AMD suspension and the new formulation, also being characterized by multiple peak plasma concentrations because of both structures of the inclusion complexes which determined prolonged and increased releases of AMD and also of different sites of absorption in biological media (digestive tract).
Data available on request
Conflicts of Interest
The authors declare that there is no conflict of interest regarding the publication of this paper.
All authors contributed equally to this work.
The authors thank Dr. Oana-Maria Mocanu for valuable support on helping us to conduct some experiments. The research leading to these results has received funding from Universitatea de Medicina si Farmacie Grigore T. Popa-Iasi through the project 29025/2016.
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Andreea Creteanu [ID], (1) Daniela Pamfil [ID], (2) Cornelia Vasile [ID], (2) Gladiola Tantaru [ID], (3) Cristina Mihaela Ghiciuc [ID], (4) Lacramioara Ochiuz [ID], (1) Alina Ghilan [ID], (2) and Ana Maria Macsim [ID] (2)
(1) Department of Pharmaceutical Technology, Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy of Iasi, 16, Universitatii Str., 700115 Iasi, Romania
(2) Physical Chemistry of Polymers Department, Petru Poni Institute of Macromolecular Chemistry, 41A Gr. Ghica Voda Alley, RO700487, Iasi, Romania
(3) Department of Analytical Chemistry, Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy, 16, University Str., 700115 Iasi, Romania
(4) Department of Pharmacology, Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 16, University Str., 700115 Iasi, Romania
Correspondence should be addressed to Andreea Creteanu; email@example.com and Cornelia Vasile; firstname.lastname@example.org
Andreea Creteanu, Daniela Pamfil, Cornelia Vasile, Gladiola Tantaru, Cristina Mihaela Ghiciuc, Lacramioara Ochiuz, Alina Ghilan, and Ana Maria Macsim contributed equally to this work.
Received 24 July 2019; Revised 18 September 2019; Accepted 25 September 2019; Published 28 November 2019
Academic Editor: Qinglin Wu
Caption: Figure 1: The aspects of the three amiodarone/2-hydroxypropyl-[beta]-cyclodextrin complexes after freeze-drying.
Caption: Figure 2: SEM images of AMD, HP-[beta]-CD, and corresponding inclusion complexes; magnification 100 [micro]m indicated on figure.
Caption: Figure 3: Size distributions of the particles in pure cyclodextrins: HP-[beta]-CD A (a), HP-[beta]-CD B (b), and HP-[beta]-CD C (c). The mean diameter is given in legend.
Caption: Figure 4: Size distribution curves of cyclodextrins and corresponding inclusion complexes: (a) of HP-[beta]-CD A, HP-[beta]-CD B, and HP-[beta]-CD C and (b) of AMD/HP-[beta]-CD A, AMD/HP-[beta]-CD B, and AMD/HP-[beta]-CD C in aqueous solution (c = 0.07 g [dL.sup.-1]) as determined by DLS.
Caption: Figure 5: ATR-FTIR spectra of the inclusion complexes of AMD with the three 2-HP-[beta]-CDs: (a) AMD/HP-[beta]-CD A, (b) AMD/HP-[beta]-CD B, and (c) AMD/HP-[beta]-CD C in respect of their components.
Caption: Figure 6: ATR-FTIR spectra of the three inclusion complexes in different spectral regions: (a) 2600-3600 [cm.sup.-1], (b) 1530-1680 [cm.sup.-1], and (c) 1200-1500 [cm.sup.-1].
Caption: Figure 7: [sup.1]H-NMR spectra of the complexes AMD/HP-[beta]-CD A and AMD/HP-[beta]-CD B and corresponding HP-[beta]-CDs dissolved in DMSO-d6 and AMD dissolved in CD[Cl.sub.3]-d.
Caption: Figure 8: The 2D NMR spectrum of the complex A.
Caption: Figure 9: UV-VIS absorption spectra of AMD at constant concentration (0.018 * [10.sup.-3] M in water) in the absence and presence of different concentrations of HP-[beta]-CD. Curves a - e for concentrations of 0.4, 0.6, 2.0, 4.0, and 6.0 mM of HP-[beta]-CD.
Caption: Figure 10: Solubility diagram of the three inclusion complexes in water: AMD/HP-[beta]-CD A, AMD/HP-[beta]-CD B, and AMD/HP-[beta]-CD C.
Caption: Figure 11: Proposed chemical structure of the inclusion complex of AMD into HP-[beta]-CD cavity.
Caption: Figure 12: The DSC curves of the three AMD/HP-[beta]-CD inclusion complexes and their components.
Caption: Figure 13: HPLC chromatograms of the three inclusion complexes: AMD/HP-[beta] A, AMD/HP-[beta] B, and AMD/HP-[beta] C and AMD.
Caption: Figure 14: Release profiles of AMD at (a) pH 1.2 and (b) pH 6.8 from the inclusion complexes comparatively with the AMD solubility curve.
Caption: Figure 15: Plasma AMD concentration versus time profiles in Wistar rats following oral administration of formulation Fc, formulation F, and pure AMD suspension (mean [+ or -] SD, n = 6).
Table 1: Characteristics of the HP-[beta]-CD and codes of the samples. Compound Abbreviation 2-Hydroxypropyl-[beta]-cyclodextrin A HP-[beta]-CD A 2-Hydroxypropyl-[beta]-cyclodextrin B HP-[beta]-CD B 2-Hydroxypropyl-[beta]-cyclodextrin C HP-[beta]-CD C Substitution degree (hydroxypropyl groups per Compound glucose unit) Mw (Da) 2-Hydroxypropyl-[beta]-cyclodextrin A 0.6 1380 2-Hydroxypropyl-[beta]-cyclodextrin B 0.8 1460 2-Hydroxypropyl-[beta]-cyclodextrin C 1.0 1540 Table 2: Matrix tablet formulations containing AMD and/or HP-[beta]- CD/AMD B complex and auxiliary excipients. Substance Formulation composition (mg/tablet) Formulation F Formulation Fc AMD 200 -- AMD/HP-[beta]-CD B -- 200 KOL 240 240 CHT 18 18 Aerosil 6 6 Stearate 3 3 Avicel Up to 600 mg Table 3: Z-average, PDI (polydispersity index), and zeta potential results. Size (nm) Sample Z-average (nm) Peak 1 Peak 2 Peak 3 HP-[beta]-CD A 1174 3.7 164 -- HP-[beta]-CD B 1172 3.6 77 530 HP-[beta]-CD C 2339 2.1 141 -- AMD/HP-[beta]-CD A 882 33 221 -- AMD/HP-[beta]-CD B 445 -- 295 -- AMD/HP-[beta]-CD C 1076 -- 189 -- Sample PDI Zeta potential (mV) HP-[beta]-CD A 0.945 -0.222 HP-[beta]-CD B 0.819 -1.43 HP-[beta]-CD C 1.000 -0.802 AMD/HP-[beta]-CD A 0.685 15.7 AMD/HP-[beta]-CD B 0.482 17.3 AMD/HP-[beta]-CD C 0.932 10.9 Table 4: DSC data for inclusion complexes and their components. Code sample [DELTA][H.sub.m] [T.sub.m] ([degrees]C) (J [g.sup.-1]) AMD 112.0 163.8 HP-[beta]-CD A 103.7 89.3 HP-[beta]-CD B 33.8 68.1 HP-[beta]-CD C 40.9 68.1 AMD/HP-[beta]-CD A 22.7 65.5 24.2 153.3 AMD/HP-[beta]-CD B 27.6 70.2 19.7 154.0 AMD/HP-[beta]-CD C 30.1 71.4 18.3 154.1 Table 5: AMD loading efficiency in inclusion complexes. Determined concentration Inclusion complex ([micro]g [mL.sup.-1]) AMD/HP-[beta]-CD A 1488 AMD/HP-[beta]-CD B 1508 AMD/HP-[beta]-CD C 1492 Average 1496 Loading efficiency of the AMD in Inclusion complex inclusion complex CE (%) AMD/HP-[beta]-CD A 97.87 AMD/HP-[beta]-CD B 99.49 AMD/HP-[beta]-CD C 96.09 Average Recovery = 97.96% Concentration of Inclusion complex the free AMD (%) Statistic results n = 6 AMD/HP-[beta]-CD A 1.45 SD = 0.373 RSD = 0.390% AMD/HP-[beta]-CD B 0.51 SD = 0.204 RSD = 0.202% AMD/HP-[beta]-CD C 3.91 SD = 0.324 RSD = 0.352% Average Table 6: Drug release parameters of the inclusion complexes (AMD/ HP-[beta]-CD A, AMD/HP-[beta]-CD B, and AMD/HP-[beta]-CD C) and pure AMD. pH 1.2 Release characteristic A B C AMD [Q.sub.max] (%) 79.3 83.2 72.1 6.2 [t.sub.r1/2] (min) 177.6 169.3 191.2 40.3 pH 6.8 Release characteristic A B C AMD [Q.sub.max] (%) 88.1 89.3 85.6 7.6 [t.sub.r1/2] (min) 138.8 136.5 140.8 4.4 [Q.sub.max]: maximum release amount; [t.sub.r1/2]: half release time. Table 7: Kinetic parameters of the AMD release from the inclusion complexes and for the AMD dissolution in the two media. First step of kinetic Samples pH release profile (0-50 min) [n.sub.r] [R.sub.n.sup.2] AMD/HP-[beta]-CD A 1.2 0.21 0.986 AMD/HP-[beta]-CD B 0.28 0.986 AMD/HP-[beta]-CD C 0.18 0.934 AMD 0.05 0.954 AMD/HP-[beta]-CD A 6.8 0.038 0.972 AMD/HP-[beta]-CD B 0.041 0.867 AMD/HP-[beta]-CD C 0.038 0.993 AMD 0.024 0.842 First step of kinetic release profile (0-50 min) K [(min).sup.-n] Samples [10.sup.2] [R.sub.k.sup.2] AMD/HP-[beta]-CD A 4.33 0.997 AMD/HP-[beta]-CD B 4.1 0.998 AMD/HP-[beta]-CD C 3.66 0.993 AMD 2.55 0.999 AMD/HP-[beta]-CD A 23.8 0.999 AMD/HP-[beta]-CD B 25.79 0.998 AMD/HP-[beta]-CD C 21.72 0.999 AMD 4.55 0.999 Second step of kinetic Samples release profile (60-120 min) [n.sub.r] [R.sub.n.sup.2] AMD/HP-[beta]-CD A 0.83 0.992 AMD/HP-[beta]-CD B 0.68 0.997 AMD/HP-[beta]-CD C 0.95 0.990 AMD -- -- AMD/HP-[beta]-CD A 0.25 0.989 AMD/HP-[beta]-CD B 0.22 0.972 AMD/HP-[beta]-CD C 0.26 0.967 AMD -- -- Second step of kinetic release profile (60-120 min) K [(min).sup.-n] Samples [10.sup.3] [R.sub.k.sup.2] AMD/HP-[beta]-CD A 3.46 0.993 AMD/HP-[beta]-CD B 7.53 0.997 AMD/HP-[beta]-CD C 1.82 0.988 AMD -- -- AMD/HP-[beta]-CD A 10.56 0.989 AMD/HP-[beta]-CD B 12.85 0.971 AMD/HP-[beta]-CD C 9.36 0.966 AMD -- -- Third step of kinetic release profile (120-300 min) Samples [n.sub.r] [R.sub.n.sup.2] AMD/HP-[beta]-CD A 1.53 0.985 AMD/HP-[beta]-CD B 1.60 0.953 AMD/HP-[beta]-CD C 1.54 0.995 AMD -- -- AMD/HP-[beta]-CD A 0.98 0.929 AMD/HP-[beta]-CD B 1.00 0.941 AMD/HP-[beta]-CD C 0.93 0.971 AMD -- -- Third step of kinetic release profile (120-300 min) K [(min).sup.-n] Samples [10.sup.3] [R.sub.k.sup.2] AMD/HP-[beta]-CD A 13.7 0.9885 AMD/HP-[beta]-CD B 51.8 0.939 AMD/HP-[beta]-CD C 2.4 0.998 AMD -- -- AMD/HP-[beta]-CD A 3.2 0.938 AMD/HP-[beta]-CD B 4.2 0.950 AMD/HP-[beta]-CD C 2.8 0.976 AMD -- -- [n.sub.r]: diffusional exponent that characterizes the drug release mechanism; [k.sub.r]: release kinetic constant; [R.sub.nr.sup.2] and [R.sub.kr.sup.2]: correlation coefficients. Table 8. Pharmacotechnical parameters of the matrix tablet formulations. Pharmacotechnical Formulation characteristics F Diameter (mm) 12.076 [+ or -] 0.010 Thickness (mm) 4.616 [+ or -] 0.016 Average mass (g) 0.576 [+ or -] 1.126 Mass uniformity (% minus) -2.777 Mass uniformity (% plus) +2.430 Dose uniformity (mg/cp) 199.42 [+ or -] 0.9981 Mechanical strength (N) 100.10 [+ or -] 3.334 Friability (%) 1.036 [+ or -] 0.019 Hydration degree (%) 115 Pharmacotechnical Formulation characteristics Fc Diameter (mm) 12.073 [+ or -] 0.012 Thickness (mm) 4.625 [+ or -] 0.030 Average mass (g) 0.595 [+ or -] 0.530 Mass uniformity (% minus) -3.333 Mass uniformity (% plus) +3.333 Dose uniformity (mg/cp) 101.71 [+ or -] 0.596 Mechanical strength (N) 99.93 [+ or -] 1.898 Friability (%) 1.054 [+ or -] 0.025 Hydration degree (%) 124 Table 9: [C.sub.max], [t.sub.max], and [AUC.sub.0-t] of pure AMD suspension and F10 and F1 formulations in Wistar rats following single oral administration (mean [+ or -] SD, n = 6). [C.sub.max] (ng/mL) Group Pure AMD Formulation Formulation suspension F Fc 1 130 216 860 2 145 229 501 3 78 374 515 4 241 319 769 5 187 261 719 6 266 99 418 Average 174.50 249.67 630.33 SD 70.87 94.50 151.37 CV (%) 40.61 37.85 24,01 [t.sub.max] (h) Group Pure AMD Formulation Formulation suspension F Fc 1 5 5 8 2 8 8 8 3 8 3 8 4 4 2 4 5 4 6 6 6 4 6 4 Average 5.50 5.00 6.33 SD 1.97 2.19 2.00 CV (%) 35.91 43.82 31.58 [AUC.sub.0-t] (mg/mL*h) Group Pure AMD Formulation Formulation suspension F Fc 1 1373.38 4925.13 21905.88 2 3954.50 3472.88 14752.50 3 2072.63 2548.25 11236.25 4 3119.00 2322.25 15540.75 5 2829.50 4197.50 16160.00 6 4440.00 2678.25 8889.00 Average 2964.83 3357.38 14747.40 SD 1142.49 1035.80 3123.50 CV (%) 38.53 30.85 21.18 [C.sub.max]: peak plasma concentration; [t.sub.max]: time to reach peak plasma concentration; [AUC.sub.0-t]: area under the curve from 0 h to last determined experimental data; SD: standard deviation; CV: coefficient of variation. Table 10: Pharmacokinetic parameters of pure AMD suspension and F and Fc0 formulations in Wistar rats following single-dose oral administration (mean [+ or -] SD, n = 6). Pharmacokinetic parameter Pure AMD suspension [C.sub.max] (ng/mL) 174.50 [+ or -]40.61 [t.sub.max] (h) 5.50 [+ or -] 1.97 [AUC.sub.0-t] (ng/mL*h) 2964.83 [+ or -] 1242.49 [AUC.sub.0-[infinity]] (ng/mL*h) 3224.06 [+ or -] 1202.28 [k.sub.e] ([h.sup.-1]) 0.053 [+ or -]0.01 [t.sub.1/2] (h) 12.88 [+ or -]0.85 [F.sub.rel] (%) Pharmacokinetic parameter Formulation F [C.sub.max] (ng/mL) 249.67 [+ or -] 94.50 * [t.sub.max] (h) 5.00 [+ or -]2.19 [AUC.sub.0-t] (ng/mL*h) 3357[+ or -]1036 [AUC.sub.0-[infinity]] (ng/mL*h) 3591[+ or -]1052 [k.sub.e] ([h.sup.-1]) 0:062 [+ or -] 0:01 [t.sub.1/2] (h) 11.46 [+ or -]2.07 [F.sub.rel] (%) 111.4 (a) Pharmacokinetic parameter Formulation Fc [C.sub.max] (ng/mL) 630.33 [+ or -]151.37 * [t.sub.max] (h) 6.33 [+ or -]2.00 * [AUC.sub.0-t] (ng/mL*h) 14747 [+ or -]3124 * [AUC.sub.0-[infinity]] (ng/mL*h) 16184 [+ or -]2924 * [k.sub.e] ([h.sup.-1]) 0.052 [+ or -]0.01 [t.sub.1/2] (h) 13.71 [+ or -]2.47 [F.sub.rel] (%) 501.9 (a) 450.1 (b) [C.sub.max]: peak plasma concentration; [t.sub.max]: time to reach peak plasma concentration; [AUC.sub.0-t]: area under curve from 0 h to last determined experimental data; [AUC.sub.0-[infinity]]: area under the plasma concentration time curve from time zero to infinity; [k.sub.e]: elimination rate constant; [t.sub.1-2]: elimination half- life; [F.sub.rel]: systemic relative bioavailability. (a) With reference to pure AMD suspension; (b) with reference to formulation F1. * p < 0.05 vs. AMD.
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|Title Annotation:||Research Article|
|Author:||Creteanu, Andreea; Pamfil, Daniela; Vasile, Cornelia; Tantaru, Gladiola; Ghiciuc, Cristina Mihaela;|
|Publication:||International Journal of Polymer Science|
|Date:||Dec 1, 2019|
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