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Structural Basis for pH-Dependent Oligomerization of Dihydropyrimidinase from Pseudomonas aeruginosa PAO1.

1. Introduction

Dihydropyrimidinase is a key enzyme for pyrimidine catabolism [1, 2]. Dihydropyrimidinase catalyzes the reversible cyclization of dihydrouracil to N-carbamoyl-[beta]-alanine in the second step of the pyrimidine degradation pathway (Figure 1). Dihydropyrimidinase can also detoxify xenobiotics with an imide functional group, ranging from linear imides to heterocyclic imides [3-9]. Homologous enzymes from microorganisms are known as hydantoinase, used as biocatalyst for hydrolysis of 5-monosubstituted hydantoins in the synthesis of D- and L-amino acids [10,11]. Optically pure amino acids have been widely used as intermediates for semisynthesis of antibiotics, active peptides, hormones, antifungal agents, pesticides, and sweeteners. Dihydropyrimidinase and hydantoinase generally possess a similar active site, but their overall sequence identity and substrate specificity may differ [3,12]. For example, hydantoinase purified from Agrobacterium species has no 5,6-dihydropyrimidine amidohydrolase activity [13]. Dihydropyrimidinases from the yeast Saccharomyces kluyveri and the slime mold Dictyostelium discoideum do not hydrolyze hydantoin [14]. Thus, several bacterial hydantoinases are still named and identified as dihydropyrimidinase because of their catalytic activity toward natural substrates, namely, dihydrouracil and dihydrothymine. These bacterial enzymes include Pseudomonas aeruginosa and Thermus sp. dihydropyrimidinases [15, 16].

Dihydropyrimidinase, hydantoinase, imidase, allantoinase, and dihydroorotase belong to the cyclic amidohydrolase family because of their functional and structural similarities [17]. Members of this enzyme family catalyze the ring-opening hydrolysis of the cyclic amide bond of each substrate in either five- or six-membered rings. Even if these enzymes have similar functions, they have relatively low amino acid sequence identity. In addition, the substrate selectivity and specificity of these enzymes highly differ [18, 19]. Most of the active sites of dihydropyrimidinases, hydantoinases, allantoinases, and dihydroorotases contain four histidines, one aspartate, and one carboxylated lysine residue, which are required for metal binding and catalytic activity [8, 15, 18, 20, 21]. The presence of a carboxylated lysine in hydantoinase is also required for the self-assembly of the binuclear metal center [12, 20, 22] and increases the nucleophilicity of the hydroxide for catalysis [23]. The global architecture of the dihydropyrimidinase monomer consists of two domains, namely, a large domain with a classic [([beta]/[alpha]).sub.8]-barrel structure core embedding the catalytic dimetal center and a small [beta]-sandwich domain [16, 22, 24, 25].

All known dihydropyrimidinases are tetramers except pseudomonal enzymes. Hydantoinase from P. putida YZ-26 functions as a dimer [26, 27]. Recently, we identified that dihydropyrimidinase from P. aeruginosa PAO1 also forms a dimer [28]. In addition, the crystal structure of P. aeruginosa PAO1 dihydropyrimidinase indicated that several residues crucial for tetramerization are not found in P. aeruginosa dihydropyrimidinase [28]. In this study, we found that the oligomerization of P. aeruginosa PAO1 dihydropyrimidinase is a pH-dependent process. At pH 5.9, P. aeruginosa PAO1 dihydropyrimidinase mainly formed a tetramer. To confirm this result and determine how this enzyme can also form a tetramer, we also determined the crystal structure of P. aeruginosa PAO1 dihydropyrimidinase at 2.17 [Angstrom] resolution at acidic environment. Structural comparison indicated that although P. aeruginosa PAO1 dihydropyrimidinase can also form a tetramer, the residues being crucial for tetramerization are different from those in Thermus sp. dihydropyrimidinases.

2. Materials and Methods

2.1. Cloning, Protein Expression, and Purification. Construction of the P. aeruginosa dihydropyrimidinase expression plasmid has been reported [15]. Recombinant P. aeruginosa dihydropyrimidinase was expressed and purified using the protocol described previously [15]. The protein purified from the soluble supernatant by [Ni.sup.2+]-affinity chromatography (HiTrap HP; GE Healthcare Bio-Sciences, Piscataway, NJ, USA) was eluted with Buffer A (20 mM Tris-HCl, 250 mM imidazole, and 0.5 M NaCl, pH 7.9) and dialyzed against a dialysis buffer (20 mM HEPES and 100 mM NaCl, pH 7.0; Buffer B). Protein purity remained > 97% as determined by SDS-PAGE (Mini-PROTEAN Tetra System; Bio-Rad, CA, USA).

2.2. Gel Filtration Chromatography. Gel filtration chromatography was carried out by the AKTA-FPLC system (GE Healthcare Bio-Sciences, Piscataway, NJ, USA). In brief, purified protein (5 mg/mL) in Buffer C (20 mM MES and 100 mM NaCl, pH 5.9) was applied to a Superdex 200 prep grade column (GE Healthcare Bio-Sciences, Piscataway, NJ, USA) equilibrated with the same buffer [29]. The column was operated at a flow rate of 0.5 mL/min, and the proteins were detected at 280 nm. The column was calibrated with proteins of known molecular weight: thyroglobulin (670 kDa), [gamma]-globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin [B.sub.12] (1.35 kDa).

2.3. Crystallography. Before crystallization, P. aeruginosa dihydropyrimidinase was concentrated to 20 mg/mL in Buffer C. Crystals were grown at room temperature by hanging drop vapor diffusion in 10% PEG 8000, 100 mM HEPES, 200 mM calcium acetate, pH 5.9. Data collection and refinement statistics for the crystal of P. aeruginosa dihydropyrimidinase are shown in Table 1. Data were collected using an ADSC Quantum-315r CCD area detector at SPXF beamline BL13C1 at NSRRC (Taiwan, ROC). All data integration and scaling were carried out using HKL-2000 [30]. There were four P. aeruginosa dihydropyrimidinase monomers per asymmetric unit. The crystal structure of P. aeruginosa dihydropyrimidinase was solved at 2.17 [Angstrom] resolution with the molecular replacement software AMoRe [31] using the dihydropyrimidinase (PDB entry 5E5C) [28] as model. After molecular replacement, model building was carried out using XtalView [32]. CNS was used for molecular dynamics refinement [33]. The final structure was refined to an R-factor of 0.1759 and an [R.sub.free] of 0.2312. Atomic coordinates and related structural factors have been deposited in the PDB with accession code 5YKD.

3. Results and Discussion

3.1. Structure of the P. aeruginosa Dihydropyrimidinase Monomer. Crystals of P. aeruginosa dihydropyrimidinase were grown at room temperature by hanging drop vapor diffusion in 10% PEG 8000, 100 mM HEPES, 200 mM calcium acetate, pH 5.9. The crystals of P. aeruginosa dihydropyrimidinase grown under this condition belonged to space group [C222.sub.1] with cell dimensions of a = 108.9, b = 155.7, and c = 235.6 [Angstrom]. The crystal structure of P. aeruginosa dihydropyrimidinase was solved at 2.17 [Angstrom] resolution (Table 1). The unit cell contained eight molecules. An asymmetric unit of the crystal contained four crystallographically independent P. aeruginosa dihydropyrimidinase monomers, in which two zinc ions were found in the active site per monomer (Figure 2(a)). The majority of the electron density for P. aeruginosa dihydropyrimidinase exhibited good quality, and no discontinuity was observed. Briefly, the overall structure of each P. aeruginosa dihydropyrimidinase unit consists of 17 [alpha]-helices, 19 [beta]-sheets, and two zinc ions (Figure 2(b)). At pH 5.9, the architecture of the P. aeruginosa dihydropyrimidinase monomer consists of two domains, namely, a large domain with a classic [([beta]/[alpha]).sub.8]-barrel structure core embedding the catalytic dimetal center and a small [beta]-sandwich domain.

3.2. Structural Comparison. The overall structure and architecture of the active site of P. aeruginosa dihydropyrimidinase are similar to those of other dihydropyrimidinases (Figure 3(a)) and other members of the amidohydrolase family of enzymes, such as hydantoinases, dihydroorotases, and allantoinases (Figure 3(b)). The active sites of these enzymes contain four histidines, one aspartate, and one carboxylated lysine residue, which are required for metal binding and catalytic activity [12, 14, 15, 19, 20, 34, 35].

3.3. pH-Dependent Oligomerization of P. aeruginosa Dihydropyrimidinase. It was noted that the crystals of the dimeric P. aeruginosa dihydropyrimidinase belonged to space group [P3.sub.1]21 grown at the condition of 28% PEG 6000, 100 mM HEPES, 200 mM lithium acetate, pH 7.5 [28]. Due to the different crystallization condition, we attempted to test whether the oligomerization of P. aeruginosa dihydropyrimidinase is pH-dependent. All known dihydropyrimidinases are tetramers. However, pseudomonal dihydropyrimidinase/hydantoinase forms a dimer at neutral pH [26-28]. Given that the structure implies that P. aeruginosa dihydropyrimidinase may also form a tetramer in the crystalline state at pH 5.9 (Figure 2(a)), we performed biochemical verification to confirm the oligomerization state. To confirm whether or not the oligomerization of P. aeruginosa dihydropyrimidinase is pH-dependent, we conducted gel filtration chromatography at pH 5.9. As shown in Figure 4, the results revealed that two species with elution volume of 63.25 and 69. 26 mL did coexist. The molecular mass of a P. aeruginosa dihydropyrimidinase monomer, as calculated from the amino acid sequence, is 53 kDa. Assuming that these two forms of P. aeruginosa dihydropyrimidinase have a shape and partial specific volume similar to the standard proteins, the native molecular masses of P. aeruginosa dihydropyrimidinase were estimated to be 105 and 180 kDa, approximately 1.9 and 3.5 times the molecular mass of a P. aeruginosa dihydropyrimidinase monomer, respectively. In comparison at pH 7.5, gel filtration chromatographic analysis of P. aeruginosa dihydropyrimidinase revealed a single peak; the native molecular mass was estimated to be 117 kDa [28]. The two forms of this enzyme obtained from the gel filtration chromatography at pH 5.9 had similar specific activity (data not shown). Thus, P. aeruginosa dihydropyrimidinase did exist as a mixture of dimers and tetramers at pH 5.9.

3.4. Structural Insights into Dimer of Dimer (Tetramer) Formation of Dihydropyrimidinase. In this study, we have identified that P. aeruginosa dihydropyrimidinase did exist as a mixture of dimers and tetramers at pH 5.9. To assess how P. aeruginosa dihydropyrimidinase can form a stable tetramer, the dimer-dimer interface was analyzed. In the crystal of P. aeruginosa dihydropyrimidinase, the four molecules formed two pairs of dimers, B-A and C-D, respectively (Figure 5). Since the two dimers of P. aeruginosa dihydropyrimidinase associate via few contacts to create the tetramer, it was thought that the tetrameric state may be possibly due to crystal packing forces. We noted that in the crystal, another crystallographically related tetramer B-AC'-D' (Figure 5) was formed and further stabilized via many hydrogen bonds and salt bridges (Tables 2 and 3). This tetramerization structure was similar to that of Thermus sp. dihydropyrimidinase (PDB entry 1GKQ).

We also compared the residues important for tetramerization located at the B-A-C'-D' dimer-dimer interface with those of Thermus sp. dihydropyrimidinase (Figure 6). Although their overall structures are similar, the important residues for tetramer (dimer B-C' with dimer A-D') formation are quite different. For the tetramer formation of P. aeruginosa dihydropyrimidinase, many hydrogen bonds with close distance were found: these bonds (<3 [Angstrom]) include K374(A)-E14(B), H13(A)-E14(B), R386(A)-E14(B), R386 (A)-E15(B), R467(A)-V354(B), R468(A)-G357(B), E14(A)-H13(B), E15(A)-R386(B), V354(A)-R467(B), G357(A)-R468(B), H13(C')-E14(D'), R386(C')-E15(D'), R468(C')-G357(D'), E14(C')-H13(D'), E14(C')-K374(D'), E15(C')-R386(D'), and G357(C')-R468(D'); however, these residues were not found for the tetramer formation of Thermus sp. dihydropyrimidinase (Figure 6). Only A13-D14 hydrogen bond was found in Thermus sp. dihydropyrimidinase (i.e., H13-E14 in P. aeruginosa dihydropyrimidinase). Thus, the dimer-dimer interface between P. aeruginosa dihydropyrimidinase and Thermus sp. dihydropyrimidinase was significantly different (Figure 7). Comparison by superimposition indicated that many Arg residues (R253, R358, R386, R467, and R468) found in P. aeruginosa dihydropyrimidinase, but not in Thermus sp. dihydropyrimidinase, may play a crucial role for the pH-dependent oligomerization. If consider the pKa, a much better candidate is His13, which is involved in intermolecular interactions and, dependent on the environment of its side chain, which may easily change protonation state between pH 5.9 and pH 7.5. However, this speculation needs to be confirmed by further biochemical experiments.

3.5. Different Mechanisms for Tetramer Formation of Dihydropyrimidinases. In this study, we identified P. aeruginosa dihydropyrimidinase can be a tetramer both in the crystalline state and in solution (Figure 4). The structure of the tetrameric Thermus sp. dihydropyrimidinase and P. aeruginosa dihydropyrimidinase was compared (Figure 6). Many important residues for Thermus sp. dihydropyrimidinase tetramer formation are different from those for P. aeruginosa dihydropyrimidinase (Figure 7). On the basis of these results, we concluded that P. aeruginosa dihydropyrimidinase could form a tetramer, but its oligomerization mechanism differed from those of other dihydropyrimidinases such as Thermus sp. dihydropyrimidinase.

https://doi.org/10.1155/2018/9564391

Conflicts of Interest

The authors declare that they have no conflicts of interest regarding the publication of this paper.

Acknowledgments

The authors thank the technical services provided by the Synchrotron Radiation Protein Crystallography Facility of the National Core Facility Program for Biotechnology, Ministry of Science and Technology, and the National Synchrotron Radiation Research Center, a national user facility supported by the Ministry of Science and Technology, Taiwan, ROC. This research was supported by a grant from the Ministry of Science and Technology, Taiwan (MOST 106-2320-B-040-004) to Cheng-Yang Huang.

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Jen-Hao Cheng, (1) Chien-Chih Huang, (1) Yen-Hua Huang, (1) and Cheng-Yang Huang (iD) (1,2)

(1) School of Biomedical Sciences, Chung Shan Medical University, No. 110, Sec. 1, Chien-Kuo N. Rd., Taichung, Taiwan

(2) Department of Medical Research, Chung Shan Medical University Hospital, No. 110, Sec. 1, Chien-Kuo N. Rd., Taichung, Taiwan

Correspondence should be addressed to Cheng-Yang Huang; cyhuang@csmu.edu.tw

Received 17 October 2017; Revised 20 November 2017; Accepted 3 December 2017; Published 30 January 2018

Academic Editor: Luigi Casella

Caption: Figure 1: The physiological reaction of dihydropyrimidinase. Dihydropyrimidinase catalyzes the reversible cyclization of dihydrouracil to N-carbamoyl-[beta]-alanine in the second step of the pyrimidine degradation pathway.

Caption: Figure 2: Crystal structure of P. aeruginosa dihydropyrimidinase. (a) Ribbon diagram of a P. aeruginosa dihydropyrimidinase tetramer. Each P. aeruginosa dihydropyrimidinase monomer is color-coded. Two zinc ions in the active site are presented as black spheres. (b) Ribbon diagram of a P. aeruginosa dihydropyrimidinase monomer with the secondary structures labeled.

Caption: Figure 3: Structural comparison. (a) Superposition of the active site of dihydropyrimidinases. Their active sites contain four histidines, one aspartate, and one carboxylated lysine residue, which are required for metal binding and catalytic activity. Dihydropyrimidinases from P. aeruginosa (PDB entry 5E5C; green), Thermus sp. (PDB entry 1GKQ; salmon), Tetraodon nigroviridis (PDB entry 4H01; pale yellow), and the structure (PDB entry 5YKD; purple blue) in this study are shown. The architecture of these active sites is similar. (b) Superposition of the active site of members of the amidohydrolase family. Their active sites contain four histidines, one aspartate, and one carboxylated lysine residue, which are required for metal binding and catalytic activity. P. aeruginosa dihydropyrimidinase (PDB entry 5YKD; purple blue), Escherichia coli allantoinase (PDB entry 3E74; bright orange), Burkholderia pickettii hydantoinase (PDB entry 1NFG; aquamarine), and E. coli dihydroorotase (PDB entry 1J79; brown) are shown. The architecture of these active sites is similar.

Caption: Figure 4: Gel filtration chromatographic analysis. Gel filtration chromatography was carried out by the AKTA-FPLC system in Buffer C (20 mM MES and 100 mM NaCl, pH 5.9). The corresponding peaks show the eluting P. aeruginosa dihydropyrimidinase. The column was calibrated with proteins of known molecular weight: thyroglobulin (670 kDa), [gamma]-globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin [B.sub.12] (1.35 kDa).

Caption: Figure 5: The structure of P. aeruginosa dihydropyrimidinase tetramer. An asymmetric unit contains four crystallographically independent P. aeruginosa dihydropyrimidinase monomers B-A-C-D. Crystallographically related tetramer B-A-C'-D' was formed and further stabilized via many hydrogen bonds and salt bridges. This tetramerization structure was similar to that of Thermus sp. dihydropyrimidinase (PDB entry 1GKQ).

Caption: Figure 6: Comparison of the tetrameric structures of Thermus sp. dihydropyrimidinase and P. aeruginosa dihydropyrimidinase. (a) Structural analysis of the dimer-dimer interface of P. aeruginosa dihydropyrimidinase. The distance ([Angstrom]) of the residues is shown. (b) Many residues crucial for forming hydrogen bonds at the dimer-dimer interface of P. aeruginosa dihydropyrimidinase were not found in the dimer-dimer interface of Thermus sp. dihydropyrimidinase.

Caption: Figure 7: Sequence alignment of dihydropyrimidinases from P. aeruginosa and Thermus sp. The amino acids that are involved in dimerdimer interface of P. aeruginosa and Thermus sp. dihydropyrimidinase are boxed, respectively.
Table 1: Data collection and refinement statistics.

Data collection
  Crystal                           P. aeruginosa dihydropyrimidinase
  Wavelength ([Angstrom])                         0.975
  Resolution ([Angstrom])                        30-2.17
  Space group                                  [C222.sub.1]
                                         a = 108.9, [alpha] = 90
  Cell dimension ([Angstrom])             b = 155.7, [beta] = 90
                                         c = 235.6, [gamma] = 120
Completeness (%)                               99.8 (100) *
<I/[sigma]I>                                   15.13 (3.7)
[R.sub.sym] or [R.sub.merge] (%)              0.122 (0.599)
Redundancy                                      7.1 (7.3)
Refinement
  Resolution ([Angstrom])                        30-2.17
  Number of reflections                           100197
  [R.sub.work]/[R.sub.free]                   0.1759/0.2312
  Number of atoms
    Protein                                        1912
    Water                                          312
RMS deviation
  Bond lengths ([Angstrom])                       0.0151
  Bond angles ([degrees])                         1.6495
Ramachandran plot
  In preferred regions                        1345 (94.19%)
  In allowed regions                            68 (4.76%)
  Outliers                                      15 (1.05%)
PDB entry                                          5YKD

* Values in parentheses are for the highest resolution shell.

Table 2: The formation of hydrogen bonds at the dimer-dimer
interface of P. aeruginosa dihydropyrimidinase.

Subunit 1         Distance [[Angstrom]]     Subunit 2

A: K374 [NZ]              3.00             B: E14 [OE1]
A: H13 [NE2]              2.88             B: E14 [OE1]
A: R386 [NH2]             3.86             B: E14 [OE2]
A: R386 [NH1]             2.81             B: E15 [OE2]
A: R386 [NH2]             2.83             B: E15 [OE2]
A: R468 [NH2]             3.61            B: Q306 [OE1]
A: R253 [NH1]             3.27             B: S307 [O]
A: R253 [NH2]             3.13             B: S307 [O]
A: R467 [NH1]             2.92             B: V354 [O]
A: R468 [NE]              2.95             B: G357 [O]
A: R468 [NH2]             3.09             B: G357 [O]
A: R468 [NH2]             3.40             B: R358 [O]
A: R467 [NH1]             3.24             B: L359 [O]
A: E14 [OE1]              3.09             B: K374 [NZ]
A: E14 [OE1]              2.47             B: H13 [NE2]
A: E15 [OE2]              2.70            B: R386 [NH1]
A: S307 [O]               3.30            B: R253 [NH1]
A: S307 [O]               3.55            B: R253 [NH2]
A: V354 [O]               2.91            B: R467 [NH1]
A: G357 [O]               2.94            B: R468 [NH2]
A: G357 [O]               2.94             B: R468 [NE]
A: R358 [O]               3.56            B: R468 [NH2]
A: L359 [O]               3.16            B: R467 [NH1]
C': H13 [NE2]             2.79            D': E14 [OE1]
C': K374 [NZ]             3.25            D': E14 [OE1]
C': R386 [NH1]            2.85            D': E15 [OE1]
C': R386 [NH2]            2.59            D': E15 [OE2]
C': R468 [NH2]            3.26            D': Q306 [OE1]
C': R253 [NH1]            3.13             D': S307 [O]
C': R253 [NH2]            3.16             D': S307 [O]
C': R468 [NE]             2.71             D': G357 [O]
C': R468 [NH2]            3.11             D': R358 [O]
C': E14 [OE1]             2.88            D': H13 [NE2]
C': E14 [OE1]             2.89            D': K374 [NZ]
C': E15 [OE2]             2.88            D': R386 [NH1]
C': E15 [OE2]             2.73            D': R386 [NH2]
C': Q306 [OE1]            3.53            D': R468 [NH2]
C': S307 [O]              3.21            D': R253 [NH1]
C': S307 [O]              3.59            D': R253 [NH2]
C': G357 [O]              2.65            D': R468 [NE]
C': R358 [O]              3.33            D': R468 [NH2]

Table 3: The formation of salt bridges at the dimer-dimer interface
of P. aeruginosa dihydropyrimidinase.

Subunit 1        Distance [[Angstrom]]     Subunit 2

A: K374 [NZ]             3.00             B: E14 [OE1]
A: H13 [NE2]             2.88             B: E14 [OE1]
A: R386 [NH2]            3.86             B: E14 [OE2]
A: H13 [NE2]             3.75             B: E14 [OE2]
A: R386 [NH1]            3.55             B: E15 [OE1]
A: R386 [NH1]            2.81             B: E15 [OE2]
A: R386 [NH2]            2.83             B: E15 [OE2]
A: E14 [OE1]             3.09             B: K374 [NZ]
A: E14 [OE1]             2.47             B: H13 [NE2]
A: E14 [OE2]             3.93             B: H13 [NE2]
A: E15 [OE1]             3.69            B: R386 [NH1]
A: E15 [OE2]             3.00            B: R386 [NH2]
A: E15 [OE2]             2.70            B: R386 [NH1]
C': H13 [NE2]            2.79            D': E14 [OE1]
C': K374 [NZ]            3.25            D': E14 [OE1]
C': H13 [NE2]            3.86            D': E14 [OE2]
C': R386 [NH1]           2.85            D': E15 [OE1]
C': R386 [NH2]           3.84            D': E15 [OE1]
C': R386 [NH1]           2.96            D': E15 [OE2]
C': R386 [NH2]           2.59            D': E15 [OE2]
C': E14 [OE1]            2.88            D': H13 [NE2]
C': E14 [OE1]            2.89            D': K374 [NZ]
C': E14 [OE2]            3.78            D': H13 [NE2]
C': E15 [OE1]            3.34            D': R386 [NH1]
C': E15 [OE2]            2.88            D': R386 [NH1]
C': E15 [OE2]            2.73            D': R386 [NH2]
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Title Annotation:Research Article
Author:Cheng, Jen-Hao; Huang, Chien-Chih; Huang, Yen-Hua; Huang, Cheng-Yang
Publication:Bioinorganic Chemistry and Applications
Date:Jan 1, 2018
Words:4542
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