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Sodium titanate--Bacillus as a new nanopesticide for cotton leaf-worm.

One of the most harmful pests which cause a great damage to several crops is the cotton leaf-worm Spodoptera Littoris (Boisd.) (Lepidoptera:Noctuidae) which is the maj or pest on cotton, corn, peanuts, vegetables, Lucerne, clover, ornamentals, shade trees and other types of crops and plants (Campion et al. 2009; Farag 2008). This worm puts hundreds of eggs in the form of egg masses and passes through six instars in its life cycle from 15-23 days (Miyahara et al. 1971). In Egypt, cotton leaf-worm is one of the most harmful and damaging pest for cotton crop and farmers used to resist it by using some traditional synthetic organophosphorous pesticides like pyrethroids and methyl parathion, but unfortunately the pest generations gained a resistance over the continuous use of these pesticides (Issa et al. 1984).

The extensive use of organochemical insecticides led to a serious types of environmental pollution (Bulmer et al. 2009; Ditta 2012; En et al. 1999; Yadav 2010). So, finding alternatives to replace these traditional pesticides has become a point of interest for a large number of researchers. Different safe alternative natural organisms have been recently used as biopesticides, from these are bacteria, fungi and viruses. Theses biopesticides have a unique mode of action (Ascher 1993; Rao et al. 1990; Thompson et al. 1999). These biopesticides are called entomopathogenic pesticides and the most used biological species from them are bacteria and fungi. They infect the digestive tract of pests (Rai and Ingle 2012).

Bacteria, Bacillus thuringiensis (Bt), is the most used biopesticide but inspite of all of these ecological and insecticidal advantages of (Bt), it has also disadvantages such as a lack of broad spectrum activity, slow rate of killing pests and low mortality percent (Hesketh and Hails 2015). The mortality % caused by (Bt) in a lab experiment was found to be about 10% only after 48hr (Servin et al. 2015). Bacillus thuringiensis was used as a commercial pesticide in USA by Edward Steinhaus since 1958 (El-ghareeb 2015).

Nanotechnology is one of the new technologies used in the agriculture field which offers the synthesis of materials having a particle size in the nanoscale with new chemical, physical , optical and magnetic properties. In addition to these properties, it has also antimicrobial and antipesticidal activities toward a variety of microorganisms and pests (Khot et al. 2012; Mohamed Ragaei and Sabry 2014; Servin et al. 2015). Nanosized particles have a wide applications in biological, physical, chemical, environmental, agricultural, industrial and pharmaceutical science (Biswal et al. 2012). Nano is a Greek word which means dwarf or very small and in scientific and mathematical field it is a metrical unit called nanometer which equals one billionth part of a meter ([10.sup.-9] m) (Biswal et al. 2012). Nanomaterials exist in different formulations (suspension, emulsion, gels, polymer based, capsules and spheres) (Kah and Hofmann 2014) and morphologies (tubes, sheets, rods, fibers and wires). This variation gives these materials a variety of surface to volume ratios which has a great effect on surface activity. So, several nanomaterials especially those of metal oxides like ; SiO2, ZnO, CuO, MnO and Ag nanoparticles have been used as nanopesticides (Servin et al. 2015). Nanopesticides may be found in the form of creams, gels or liquids. It has a long shelf life time and more killing activity toward the targeted pests (Yang et al. 2007).

Bioinsecticides are currently studied more and more because of the possibility of their use in plant protection as an alternative method to the broad use of conventional toxic and polluting pesticides. On the other hand, nanomaterials in different formulations are now being used but over a limited range of use. Nanopesticides may consist of organic ingredients (polymers) and/ or inorganic ingredients such as metal oxides in various forms (particles and micelles). The use of metallic nanoparticles like carbon (C), silver (Ag), copper oxide (CuO), zinc oxide (ZnO), iron oxide (FeO) and titanium oxides (Ti[O.sub.3], Ti[O.sub.2]) are the most used nanoparticles in plant protection field (M. Ragaei and A. Sabry 2014). Take for example CdSe, Ag and Ti[O.sub.2] nanoparticles when used as nanopesticides against Spodoptera Littoralis, it was found that they increase the larval mortality in the following trend CdSe, Ti[O.sub.2] and Ag respectively (M. Ragaei and A. Sabry 2014). Hydrophobic silica nanoparticles come to achieve high mortality percent at 300 and 350ppm concentrations ,respectively, when they are used for tomato to resist Spodoptera Littoralis (H. M. El-bendary and A. A. El-Helaly 2013). Silica nanoparticles leave no pesticidal residues in food or soil and can be used with other pest management activities (Laing, M. D. and M. C. Gatarayiha 2006).

The aim of the present work is to examine the use of sodium titanate nanotubes (TNTs) as a new nanopesticide and to enhance the activity of the ecofriendly Bacillus thuringiensis (Bt) based biopesticides via making nanocomposite between it and (TNTs) to be used as a nanopesticide against cotton leaf-worm Spodoptera Littoris (Boisd.) (Lepidoptera:Noctuidae).

MATERIALS AND METHODS

Chemicals

Ti[O.sub.2] powder was purchased from Luba Chemie-India, sodium hydroxide and hydrochloric acid were purchased from EL Nasr Company-Egypt and Bacillus was obtained from Biopesticides unit-Agricultural research center (ARC)--Egyptian ministry of agriculture.

Insect rearing

The cotton leaf worm, Spodoptera littoralis was reared in the laboratory for several generations at room temperature ranged between 25-28[degrees]C and 60-65% R.H. Larvae were fed on castor bean leaves, Ricinus communis (L.) in wide glass jars until pupation period and adults emergence. The newly emerged adults were mated inside glass jars supplied with a piece of cotton wetted with 10% sugar solution as a feeding source for the emerged moths and branches of Tafla (Nerium oleander L.) or castor bean leaves as an ovipositor site (Mansour, N. A. 1966). Egg masses were kept in plastic jars until hatching. The obtained second and fourth instars larvae were used for bioassay tests. The bioassay evaluations were performed under the same laboratory condition for 12h photophase.

Synthesis of nanocompounds

Synthesis of sodium titanate nanotubes

All the reactants used were of analytical grade and were used without further purification. Five grams of pure bulk anatase Ti[O.sub.2] powder was mixed with 250 ml 10M aqueous NaOH solution under magnetic stirring for about 45 min till a milky white solutions were obtained. Then, the obtained solution were transferred to 500 ml capacity teflon-lined stainless steel autoclave, and after that temperature treatment was carried out at 160 [degrees]C for 16 hr for the preparation of sodium titanate nanotubes. The autoclave chamber was allowed to cool down till it reached to room temperature. The formed white precipitate was collected and washed several times using distilled water(A A Farghali et al. 2014; Ahmed A. Farghali et al. 2016).

Synthesis of Bt-TNTs anate nanocomposite

The composite of sodium titanate with (Bt) was prepared in the ratio of 1:2gm nanomaterial and bacillus, respectively. The powders were mixed according to this ratio in 200 ml distilled water, sonicated for 10 min, stirred for 1hr using magnetic stirrer and finally dried at 50[degrees]C for 24 hr.

Characterization

Sodium titanate, Bacillus and their nanocomposite were characterized by X-ray diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM), Zetasizer for zeta potential measurements and FT-IR spectroscopy.

Bioassay

A weighted amount of 0.25gm powder of sodium titanate (TNTs), Bacillus (Bt) and their nanocomposite (Bt-TNTs) was dissolved in 250 ml distilled water. After that we studied the impacts appeared on the different biological features of 2nd and 4th instars larvae of S. littoralis after feeding on castor bean leaves (Ricinus communis) (Schuster 1973) treated with these materials using dipping method. larval and pupal mortality, pupation and adult emergence %, larval and pupal duration, fecundity, eggs hatching, adult longevity and sex ratios were studied for 48hr. Four replicates (i.e. eight groups devided to four sets) each of 2nd and 4th instars of S.littoralis were prepared to contain 10 larvae for each replicate. The replicates were placed over a sawdust inside a transparent plastic can (10x10x4[cm.sup.3]). The larvae in the first three replicates were fed on the recinus leaves immersed in the prepared solutions of (TNTs, Bt-TNTs) and Bacillus (Bt), respectively, whereas the larvae in the fourth replicate were fed on untreated leaves (control sample).

Statistical analysis

The total percent of larval mortality of 2nd and 4th instars larvae until pupation was recorded and corrected using Abbott's formula (Abbott 1925) and different biological parameters were evaluated at the tested concentration. The obtained data of the biology were statistically analysed to determine F-value, P-value and L.S.D (least significant difference) at 0.05 and 0.01 degrees of freedom.

RESULTS AND DISCUSSION

Physical characterization

Figure 1 shows the XRD patterns of all samples, it is clear from patterns that the mean peaks of the as prepared samples Na-titanate nanotubes (TNT); at 9.8[degrees], 24.2[degrees], 28.2[degrees], 48.2[degrees]; are the characteristic peaks of the tubular titanate. This figure also shows the XRD pattern of Bacillus bacteria where many peaks are observed at 18[degrees], 22[degrees], 31.9[degrees], 45.3[degrees] which may be attributed to the presence of crystalline proteins in this bacteria. While in case of Bt-TNT only the peaks of TNT and TNS can be observed with some change in their intensities and this may be attributed to the coverage of the Bacteria surfaces with TNT and TNS.

Figure (2-a) shows (FESEM) picture of (TNTs) at the nanoscale appearing as agglomerations and bundles of nanotubes and figure (2-b) shows (FESEM) of Bacillus thuringiensis (Bt) colony. Figure (2-c) shows (FESEM) picture of (Bt-TNTs) nanocomposite and demonstrates the adsorption of nanotubes on the bacterial surfaces revealing the success of interaction between them and explains the difference in the bacterial activity after composite formation.

Latent effect

Total mortality, pupation and emergence

The data presented in Table (1) show 20 % total mortality percent for both 2nd and 4th instars in case of (BT-TNTs) composite while Bacillus Thuringiensis (Bt) resulted in 30% total mortality compared to 0% in case of control. The table also illustrate that all treatments showed 100% pupation except (Bt) which caused 90% pupation for the 4th instar.

It is obvious also from the data presented in table (1) that (Bt-TNTs) resulted in 80% emergence for both 2nd and 4th instars. There was also a decrease in emergence percent to be 70% for the 4th instar in case of (Bt) alone compared to 100% emergence for both 2nd and 4th instars in case of control sample. There was no effect on the emergence % after using (Bt) for the 4th instar and (TNTs) for both instars.

Larval and Pupal duration %

The data presented in table (2) illustrate the deviation in larval duration period compared to that of control sample. The use of (TNTs) led to 4 and 25% decrease in larval duration for 2nd and 4th instars, respectively. On the other hand (Bt-TNTs) caused 11% increase in larval duration period for the 2nd instar. Bacillus Thuringiensis (Bt) treatment led to 16 and 18% larval duration increase for 2nd and 4th instars, respectively, compared to control. The table also illustrates that (TNTs) treatment resulted in 7 and 18% pupal duration increase for the 2nd and 4th instars, respectively, while its composite (Bt-TNTs) led to 32% pupal duration increase for the 4th instar. There was no effect on the pupal duration% after using (Bt).

Malformations

Table (2) shows that there was no effect of all samples on the larval and pupal malformation of both 2nd and 4th instars. TNTs resulted in 20 and 30% adult malformation for 2nd and 4th instars, respectively, while (Bt) caused 12.5% adult malformation for the 4th instar. Bt-TNTs showed no effect on the adult malformation %.

Adult fecundity and hatchability %

Table 3 show that (TNTs) resulted in 37 and 72% adult fecundity decrease for 2nd and 4th instars, respectively, while (Bt-TNTs) resulted in 42% fecundity increase and 82% fecundity decrease for 2nd and 4th instars, respectively. On the other hand, (Bt) alone resulted in 79 and 86 % adult fecundity decrease for 2nd and 4th instars, respectively, compared to control.

The data presented in table 3 show 34.9% hatchability for the 2nd instar after using (TNTs) and 16.7% hatchability for the 4th instar after using (BtTNTs) while (Bt) resulted in 76.7% hatchability for the 2nd instar only compared to 100% in case of control.

Adult longevity and Sex ratio

The data presented in table 4 illustrate 12 and 4% adult longevity decrease for 2nd and 4th instars, respectively, after using (TNTs) whereas (Bt-TNTs) caused 33% adult longevity increase and 22% decrease for 2nd and 4th instars, respectively. The treatment using (Bt) resulted in 7% adult longevity increase and 7% decrease for 2nd and 4th instars, respectively, compared to that of control.

Also the data shown in table 4 illustrate the effect of the prepared nanomaterials on the adult sex ratio of females (F) and males (M). The adult sex ratio was (30 F : 70 M), (62.5 F : 37.5 M) and (60 F : 40 M) after using (TNTs), (Bt-TNTs) and (Bt), respectively, for the 2nd instar compared to (40 F : 60 M) in case of control. It is obvious also from the table that the sex ratio of the 4th instar was (40 F : 60 M), (62.5 F : 37.5 M) and (50 F : 50 M) after using (TNTs), (Bt-TNTs) and (Bt), respectively, compared to (60 F : 40 M) of control.

All the previous results match to a great extend those results obtained by Salama et al. (1981) who studied the effect of Bacillus Thuringiensis Kurustaki (Bt) on some biological parameters of three Lepidopterous cotton pests such as larval and pupal mortality, larval and pupal period which are increased on using (Bt) specially at low toxin concentration (Salama et al. 1981). Also, pupal and larval weight are reduced under the effect of (Bt) treatment (Arshad et al. 2009; Ashfaq et al. 2001; Salama et al. 1981). In addition to that, weak effect of (Bt) on fecundity, hatchability, pupal and adult deformation was observed (Abdel-Rahim 2011; Salama et al. 1981). Moreover, the bacterial nanocomposites show an improvement in the larval activity and a stronger effect on the pest different biological features than bacteria alone.

CONCLUSION

The present work proved the promising use of sodium titanate nanotubes and its nanocomposite with Bacillus Thuringiensis as a new nanopesticide against cotton leaf-worm Spodoptera littoralis (Boisd.) (Lepidoptera:Noctuidae) and how they affected the different biological features such as larval and pupal duration, adult fecundity, larval and pupal malformation, adult longevity and adult sex ratio.

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A.M. Zaki (1), A.H. Zaki (2), A.A. Farghali (2), and Elham F. Abdel-Rahim (1)

(1) Sids Agric. Res. Station, Plant Protection Res. Institute, ARC, Giza, Egypt.

(2) Materials Science and Nanotechnology Dept., Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt.

http://dx.doi.org/ 10.22207/JPAM.11.2.11

(Received: 02 May 2017; accepted: 15 June 2017)

* To whom all correspondence should be addressed. E-mail: ayman_h_zaki@yahoo.com

Caption: Fig. 1. XRD patterns of Bt, TNTs and Bt-TNTs nanocomposites

Caption: Fig. 2. FESEM monographs of a-) TNTs, (b) Bt and (c) Bt-TNTs
Table 1. Effect of different samples on larval and pupal mortality %,
pupation and emergence % of the 2nd and 4th instars of Spodoptera
littoralis

Samples             % of         % of Pupal               Total
                    larval       mortality    Mortality
                   mortality                               %

               2nd       4th        2nd          4th       2nd
             instar     instar     instar      instar     instar

(TNTs)          0         0          0            0         0
(Bt-TNTs)       0         0          20          20         20
(Bt)            0         10         0           20         0
Control         0         0          0            0         0

Samples             %         Pupation             Emergence
                                            %

             4th      2nd        4th       2nd        4th
            instar   instar    instar     instar    instar

(TNTs)        0       100        100       100        100
(Bt-TNTs)     20      100        100        80        80
(Bt)          30      100        90        100        70
Control       0       100        100       100        100

Table 2. Effect of different samples on larval and pupal duration and
malformations of 2nd and 4th instars of S. littoralis.

Samples                          Larval duration
                                  Mean [+ or -]
                                    S.D. (Days)

                         2nd                    4th
                        instar                instar

(TNTs)          18.5 [+ or -] 3.8 n.s.   14.2 [+ or -] 0.6
                                                 j
(Bt-TNTs)             21.3+3.5*          18.9 [+ or -] 2.1
                                                n.s
(Bt)              22.2 [+ or -] 4.3      22.3 [+ or -] 5.2
                          **                    **
Control           19.2 [+ or -] 1.6      18.9 [+ or -] 1.5
F value                 7.438                  17.75
P value                 0.0298                 0.03
L.S.D at 0.05           1.513                  1.48
0.01                    2.128                   2.1

Samples                Larval
                       mallo. %

                  2nd       4th
                 instar    instar

(TNTs)             0         0

(Bt-TNTs)          0         0

(Bt)               0         0

Control            0         0
F value
P value
L.S.D at 0.05
0.01

Samples                     Pupal duration
                             Mean [+ or -]
                              S.D. (Days)

                       2nd                 4th
                     instar              instar

(TNTs)           18 [+ or -] 3.1     20.1 [+ or -] 1.5
                      n.s.               (a) **
(Bt-TNTs)       18.6 [+ or -] 1.5   22.4 [+ or -] 1.6
                       **
(Bt)            16.8 [+ or -] 2.3    17 [+ or -] 4.4
                       n.s                 n.s
Control         16.9 [+ or -] 0.3         0.317
F value              42.941              95.546
P value              0.00922              0.01
L.S.D at 0.05        0.6375               0.892
0.01                 0.9275               1.268

Samples             % Pupal        Adult Malfo. %
                    malfo.

                 2nd      4th      2nd      4th
                instar   instar   instar   instar

(TNTs)            0        0        20       30

(Bt-TNTs)         0        0        0        0

(Bt)              0        0        0       12.5

Control           0        0        0        0
F value
P value
L.S.D at 0.05
0.01

Table 3. Effect of different samples on adult fecundity and eggs
hatchability

Samples             Adult fecundity (eggs/female)
                          Mean [+ or -] S.D.

                    2nd instar            4th instar

(TNTs)          71.7 [+ or -] 30 *    37.3 [+ or -] 32 **
(Bt-TNTs)       160 [+ or -] 53n.s    24 [+ or -] 3.6 **
(Bt)            25 [+ or -] 16 **    19.3 [+ or -] 10.1 **
Control          113 [+ or -] 23       133.3 [+ or -] 2
F value              127.0408               33.0572
P value                0.02                 0.0324
L.S.D at 0.05         17.77                 41.1417
0.01                  32.622                75.518

Samples                 Eggs hatchability%

                    2nd instar       4th instar

(TNTs)                 34.9             zero
(Bt-TNTs)              zero             16.7
(Bt)                   76.7             zero
Control                100              100
F value
P value
L.S.D at 0.05
0.01

Table. 4. Effect of different samples on adult longevity and sex
ratios.

Samples                    Adult longevity Mean
                               [+ or -] S.D.

                     2nd instar             4th instar

(TNTs)          7.5 [+ or -] 0.7 n.s   7.8 [+ or -] 0.9 n.s
(Bt-TNTs)       10.9 [+ or -] 21 **    6.3 [+ or -] 1.6 **
(Bt)            8.8 [+ or -] 1.5n.s     6.3 [+ or -] 2 **
Control           8.2 [+ or -] 1.0       8.1 [+ or -] 1.7
F value               20.2557                10.8188
P value                0.0233                0.022965
L.S.D at 0.05           0.77                   1.05
0.01                   1.102                  1.502

Samples                           Sex ratios %

                    2nd instar                 4th instar

                Females           Males    Females          Males

(TNTs)                30           70           40           60
(Bt-TNTs)            62.5         37.5         62.5         37.5
(Bt)                  60           40           50           50
Control               40           60           60           40
F value
P value
L.S.D at 0.05
0.01
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Author:Zaki, A.M.; Zaki, A.H.; Farghali, A.A.; Abdel-Rahim, Elham F.
Publication:Journal of Pure and Applied Microbiology
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Date:Jun 1, 2017
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