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Sistematizacao da arteria testicular em caprinos segundo a configuracao escrotal.

Testicular arteries systematization based on different levels of scrotal configuration in caprines

INTRODUCTION

Caprines live harmoniously in adverse environmental of the tropical regions, showing efficient mechanisms of self-adaptation. The angioarchitecture of the testicle plays an important role in the thermoregulation of this organ in many species, such as in bovine (HESS et al., 1990) and ovine (PASCUALY & ELOY, 1985).

In native caprines that have been born in Brazilian Northeast region, it was reported to have a two-compartment scrotum division, conferring to them a greater volume and number of ejaculated spermatozoa when compared to caprines that possess an entire scrotum (PASCUALY & ELOY, 1985)

It is not well understood the correlation between bipartition in the scrotum in caprines and testicular thermoregulation. In the literature, mainly concerning ovine species, descriptions about the vascular arrangement of the spermatic funiculus have been observed (NOORDHUIZEN-STASSEN et al., 1985; CARVALHAL et al., 2000) and little attention has been given to caprine species (SOUZA et al., 1992; OLIVEIRA et al., 2002). No report was found on vascular configuration in caprines testicles with scrotal bipartition.

The objective of the present study was to analyze the origin and the distribution of the arterial vessels in caprines testicles with different degrees of scrotal division.

MATERIALS AND METHODS

This research was conducted at the Centre of Agrarian Science at the Federal University of Piaui, Brazil. Thirty native caprines without defined breed, from 8 to 24 months of age, from the Brazilian state of Piaui were used in this study.

Three groups of ten animals each were classified according to the scrotal configuration as follows: scrotum without bipartition (group I); scrotum showing a ventral division until the middle of the testis (group II); and, scrotal separation extended beyond the middle of the testis (group III).

Testicles were removed from abdominal cavity to evaluate the origin and the distribution of the testicular arteries. After dissection, vessels were canulated with 20-gauge needle and vascular system was washed with saline solution 0.9%. Samples were injected with 3mL of acetone in each artery followed by an injection with 3mL solution of vinyl acetate (VMCH-B:1099--Union Carbide Corporation, Chemical and Plastic) colored with a nitrocellulose red-molibdate lacquer dye (Glassurite do Brazil S.A.--Paint Industry--Brazil). Samples were washed in running water for a period of three hours, immersed in 30% sulfuric acid solution for 72- to 96-hour period, and then washed with distilled water. This technique allowed us to maintain vessels vascular structure.

Corrosion cast were used to evaluate morphology of testicular artery and the number of cranial, caudal and penetrating branches. The distributions of the vessels in the dorsomedial (DM), ventromedial (VM), dorsolateral (DL) and ventrolateral (VL) quadrants were achieved through an imaginary trace of two planes perpendicular among themselves. A long axis longitudinally to each testis was used to delimit the lateral and medial halves, and other axes horizontally to the testis were used to delimit the dorsal and ventral half. Images were captured using a Nikon digital photographic camera.

Numeric values were submitted to analysis of variance and their means compared by Duncan's test. Statistical Analysis System (SAS) software was used and P-value was set at 0.05.

RESULTS

Origin of the testicular artery

Thirty pairs of caprine testicles were used in this experiment. After abdominal dissection we observed that, in all cases, the origin of the testicular arteries is situated in the ventral surface of the abdominal portion of the aorta. These arteries showed a close relationship to the caudal mesenteric artery and the right testicular artery more cranial (53.3%), or more caudal (20.0%) or at the same level (26.7%) to the left testicular artery.

Testicular arteries showed a rectilinear itinerary in the ventrocaudal direction towards to the vaginal opening and together with veins and ductus deferens as part of the spermatic funiculus. At this point, the arteries are coiled until they reach the cranial extremity of the testis (Figure 1A). In this funicular segment, the testicular artery emits two epididymal branches (Figure 1B), one lateral and another medial, which can be originated from the common (52.2%) or the independent (47.8%) trunk.

The epididymis lateral artery is predominantly responsible for the vascularization of the epididymis head, while the medial artery vascularizes the body and the tail of the epididymis. However, in all analyzed testicles the lateral vessels emit branches to the epididymis body and the medial artery vessels emit branches to the epididymis head. The lateral branches arise dorsally to the medial branches of the testicles in 56.25%, ventrally in 12.5% and at the same level in 31.25% of the cases studied.

Ramification and distribution of the testicular arteries At the cranial extremity of the testis, the testicular arteries are superficially located throughout the tunica albuginea. The pampiniform plexus represented by a fine network of testicular veins almost completely involves the segment of the testicular artery in the spermatic funiculus. These testicular veins are prolonged to embrace the epididymis arterial branches and to give a conical shape to the funiculus to build part of the cranial extremity of the testis.

[FIGURE 1 OMITTED]

Testicular artery reaches the caudal extremity of the testis and becomes divided in cranial and caudal branches in 80% of the samples (Figure 1C), they will originate a variable number of cranial (1 to 6) and caudal branches (1 to 4) in 20% of the remaining samples (Figure 1D).

In our study, the cranial and caudal arterial branches did not surround the entire testis. These arterial branches form collateral branches that continue into the parenchyma until reach the testicular mediastinum where they are called penetrating branches. The collateral branches are observed at the caudal branch in 48.8%, at the cranial branch in 39.5%, and at the same level of bifurcation in 11.6% of the cases. The cranial collateral branches supply the cranial region of the testicle (39.5%) and the caudal collateral branches contribute to the caudal region in 9.3% of the cases.

The mean values of penetrating branches originated from the cranial and caudal arterial branches are presented in table 1. The number of total animals classified in Group I showed a tendency to have a larger number of penetrating cranial and caudal branches when compared to animals located in Group II and Group III, although the data could not be statistically confirmed due to the small sampling used in our study.

The means values of penetrating branches according to their distribution in the dorsomedial (DM), dorsolateral (DL), ventromedial (VM) and ventrolateral (VL) quadrants, are shown in table 2. All the animals showed a large amount of the penetrating branches with different degrees of scrotal division in caprine testicles. The total amount of penetrating branches in caprine testicles with different scrotal division levels is shown in table 3.

DISCUSSION

The origin of the testicular arteries in the ventral surface of the abdominal portion of the aorta, close to the caudal mesenteric artery, is in agreement with GETTY'S (1986) findings in ruminants. In the literature, BARONE (2003) reported that the right testicular artery could be originated from the renal artery in caprines. It was observed that 53.3% of the paired testis had the right testicular artery more cranial (53.3%), or more caudal (20.0%) or at the same level (26.7%) of the left testicular artery.

The distribution of testicular arteries in caprines were similar to described for bovines (HESS et al., 1990; CARVALHAL et al., 2000) and buffalos (MACHADO et al., 1996). The present study showed that lateral and medial epididymis branches originate from a common or separated trunk, although MACHADO et al., (1996) described as a unique branch in buffalos.

In bovines, the branch that supplies the head portion of the epididymis is denominated "cranial branch", and the one that supplies the body is called "caudal branch" of the epididymus (DYCE et al., 1997). There are no reports in the literature describing the level in which the lateral branch is originated from the medial testicular artery.

The fine network of the testicular veins in the pampiniform plexus evaluated by this study agrees with that described by different authors in bovines (HESS et al., 1990; MACHADO et al., 1996; CARVALHAL et al., 2000). The conical shape of the spermatic funiculus in the testis is similar to the description made by CARVALHAL et al. (2000) in ovines.

Cranial and caudal testicular arteries branches were observed in caprines (OLIVEIRA et al., 2002) and in buffalos (PASSIPIERI et al., 1998). Sinuous branches, originated from cranial and caudal arterial branches, are denominated testicular or collateral branches by RODRIGUES & BORELLI (1990) and SOUZA et al. (1992), the same classification adopted in the present study.

In groups I and III, the total number of testicular penetrating branches in each particular quadrants differs from RODRIGUES & BORELLI (1990) observations in ovine, where the most populated quadrant was the dorsomedial and the less populated was the ventrolateral in the right and left testicles. The most populated quadrants in the group II (dorsolateral and ventrolateral) differ from the study described by RODRIGUES & BORELLI (1990) in ovine species. Among the group II, vascular casts showed that the ventromedial quadrant had less number of branches than the dorsomedial quadrant.

The similar number of penetrating branches among the right and left testicles were also observed in ovine by RODRIGUES & BORELLI (1990); however, results that compare the testicular vascularization among the animals with different levels of scrotal division were not found in the literature.

In summary, macroscopic study showed that the origin, distribution and localization of the testicular arteries are similar to other domestic ruminants, and they do not show any differences related to the degree of scrotal division in caprines.

ACKNOWLEDGEMENTS

This study was supported by The National Council for Scientific and Technological Development (CNPq), no.478080/03-4.

Received 17.07.07 Approved em 02.01.08

REFERENCES

BARONE, R. Trattato di anatomia comparata dei mammiferi domestici. Angiologia: cuore e arterie, Bologna: Edagricole, 2003. 5v.

CARVALHAL, R. et al. Estudo morfologico do funiculo espermatico em ovinos da raca Corriedale (Ovis aries, L. 1758). Brazilian Journal of Veterinary Research and Animal Sciense, Sao Paulo, v.37, n.1, p.348-354, 2000.

DYCE, K.M. et al. Tratado de anatomia veterinaria. Rio de Janeiro: Guanabara, 1997. 1v.

GETTY, R. Sisson/Grossman anatomia dos animais domesticos. Rio de Janeiro: Interamericana, 1986. 2v.

HESS, H. et al. Vascular morphology of the bovine testis. Anatomischer Anzeiger, Helle, v.170, p.119-132, 1990.

MACHADO, M.R.F. et al. Contribution to the study of the funiculus spermaticus in murrah buffaloes (Bubalus bubalis--LINNAEUS, 1978). Brazilian Journal Veterinary Research and Animal Science, Sao Paulo, v.13, n.1, p.7-13, 1996.

NOORDHUIZEN-STASSEN, E.N. et al. Functional arterio-venous anastomoses between the testicular artery and the pampiniform plexus in the spermatic cord of rams. Journal Reproduction and Fertility, Southamptam, v.75, p.193-201, 1985.

OLIVEIRA, R.R. et al. Aspectos morfometricos e irrigacao arterial do escroto e testiculo em caprinos nativos do estado de Pernambuco. Revista Brasileira de Reproducao Animal, Belo Horizonte, v.5, p.129-131, 2002.

PASCUALY, O.S.; ELOY, A.X. Evaluacion de parametros reproductivos en machos ovinos y caprinos en el CNPC. Sobral: IICA/EMBRAPA/CNPC, 1985. 22p. (Boletim Tecnico).

PASSIPIERI, M. et al. Contribuicao ao estudo da vascularizacao arterial do testiculo de bufalos da raca Murrah. Brazilian Journal Veterinary Research and Animal Science, Sao Paulo, v.35, p.24-66, 1998.

RODRIGUES, C.A., BORRELLI, V. Contribuicao ao estudo da vascularizacao arterial do testiculo em ovinos da raca Corriedale (Ovis aries Linnaeus, 1758). Brazilian Journal Veterinary Research and Animal Science, Sao Paulo, v.27, p.268-274, 1990.

SOUZA, M.R.Q. et al. Contribuicao ao estudo da vascularizacao arterial do testiculo em caprinos (Capra hircus--Linnaeus, 1758), da raca Bhuj Brasileira. Ars Veterinaria, Porto Alegre, v.8, p.100-117, 1992.

Monica Marco de Almeida (I), Antonio Chaves de Assis Neto (II)* Alexandra Klindline Penno (I) Airton Mendes Conde Junior (I) Danilo Jose Ayres de Menezes (III) Gabriel Ribas Pereira (IV) Laurita Martins de Azevedo (I) Maria Acelina Martins de Carvalho (I)

(I) Departamento Morfologia Veterinaria, Centro de Ciencias Agrarias, Universidade Federal do Piaui (UFPI), Bairro Ininga, Teresina, PI, Brasil.

(II) Unidade Experimental de Dracena, Universidade Estadual Paulista "Julio de Mesquita Filho", Rod. Cmte. Joao Ribeiro de Barros, 294, km 651, 17900-000, Dracena, SP, Brasil. E-mail: antonioassis@dracena.unesp.br. *Autor para correspondencia.

(III) Unidade Academica de Medicina Veterinaria, Universidade Federal de Campina Grande (UFCG), 58700-970, Patos, PR, Brasil.

(IV) Department Health Population and Reproduction, School of Medicine Veterinary, 1114, Tupper Hall, University of California Davis, 95616, CA, USA.
Table 1 - Mean values and respective standard deviations
corresponding to the number of penetrating vessels
derived from the cranial and caudal branches with
defferente degrees of scrotal division in caprines.

Branches       Group I            Group II

Cranial    17 [+ or -] 7.35    9 [+ or -] 3.83
Caudal     19 [+ or -] 2.12   15 [+ or -] 7.78
Total      41 [+ or -] 4.95   31 [+ or -] 7.78

Branches      Group III

Cranial    12 [+ or -] 8.42
Caudal     16 [+ or -] 9.96
Total      28 [+ or -] 17.66

Statistical analysis using Duncan's test (P > 0.05). Scrotum
without bipartition (group I); Scrotum showing a ventral division
until the middle of the testis (group II); and Scrotum separation
extended beyond the middle of the testis (group III).

Table 2 - Mean and relative standart deviations of the
penetrating branches with different degrees of scrotal
division in caprines testicles.

Quadrante       Group I             Group II

DM          4 [+ or -] 3.23b    1 [+ or -] 1.43c
DL         11 [+ or -] 4.33a    6 [+ or -] 2.95a
VM          6 [+ or -] 4.46b    3 [+ or -] 2.10b
VL         11 [+ or -] 7.23a    5 [+ or -] 3.69a

Quadrante      Group III

DM          2 [+ or -] 2.40b
DL         10 [+ or -] 6.29a
VM          4 [+ or -] 2.32b
VL         10 [+ or -] 8.78a

Means followed of different letters in the same column differ
between it self by Duncan's test (P < 0.05). DM: dorsomedial
quadrant; DL: dorsolateral quadrant; VM: ventromedial quadrants;
VL: ventrolateral quadrant. Scrotum without bipartition (group I);
Scrotum showing a ventral division until the middle of the testis
(group II); and Scrotum separation extended beyond the middle of
the testis (group III).

Table 3 - Mean and respective standard deviations relative
to the total amount of penetrating branches in caprines
testicles with different scrotal division levels.

Groups   Penetrating branches

I         32 [+ or -] 14.22a
II        15 [+ or -] 7.29b
III       26 [+ or -] 16.92a

Means followed of different letters in the same column differ
between it self by Duncan's test (P < 0.05). Scrotum without
bipartition (group I); Scrotum showing a ventral division until
the middle of the testis (group II); and Scrotum separation
extended beyond the middle of the testis (group III).
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Author:Marco de Almeida, Monica; de Assis Neto, Antonio Chaves; Klindline Penno, Alexandra; Mendes Conde, A
Publication:Ciencia Rural
Date:Aug 1, 2008
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