Silica nanoparticle-based zinc sensor for in vitro study of intracellular zinc.
Zn(II) sensors are very popular because of its diverse role within biological systems. For instance, it is a necessary component in DNA synthesis and apoptosis. It also plays an important role in protein function. Zinc is also related to some diseases it has been found that the zinc concentration is very high for Alzheimer's patients.
Nanotechnology presents exciting and truly revolutionary approaches to address global challenges in the scientific world. A unique property of the nanoparticles is their size, resulting in two useful features, high mobility and high surface area to volume ratio. These features provide nanoparticles with very high sensitivity as a signaling reagent when combined with target-induced fluorescent compounds. By binding about 15,000 CDAPAP molecules to a single nanoparticle, the fluorescence signal can be localized for enhancement of the signal of low levels of Zn(II) present. This is particularly useful when using the fluorescence microscope to image cellular samples, and to monitor change over time.
Carrie L. Amiot, Yuhui Jin, David T. Pierce, Julia Xiaojun Zhao
University of North Dakota
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|Author:||Amiot, Carrie L.; Jin, Yuhui; Pierce, David T.; Zhao, Julia Xiaojun|
|Publication:||Proceedings of the North Dakota Academy of Science|
|Date:||Apr 1, 2008|
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