Shimadzu releases LC/MS/MS Sample Prep Kit.
Shimadzu Scientific Instruments (SSI) has released the nSMOL (nano-surface and molecular orientation limited proteolysis) Antibody Bio-Analysis Kit, applicable to a variety of pharmaceutical antibodies. This technique enables selective proteolysis of the Fab region of monoclonal antibodies to improve the productivity and robustness of LCMS mAB bioanalysis.
The nSMOL Antibody Bio-Analysis Kit is a ready-to-use reagent kit for collecting monoclonal antibodies from blood or other biological samples using immunoglobulin collection resin, and then performing selective proteolysis of the Fab region of these antibodies via trypsin-immobilized nanoparticles. Variable region-derived peptides produced by limited proteolysis can then be quantified via MRM measurements utilizing Shimadzu's LCMS-8050/8060 triple quadrupole liquid chromatograph mass spectrometer.
The simplified workflow eliminates the steps of denaturing, reduction, and alkyation normally associated with protein digestion, resulting in more efficient sample preparation and analysis, according to the company.
With the nSMOL Antibody Bio-Analysis Kit, the region of the antibodies subject to analysis (Fab region) is selectively fragmented. According to the company, this results in a sample preparation protocol that dramatically suppresses background noise and ion suppression, which leads to improved response and quantitative repeatability. In addition, the selective collection of Fab peptides limits contamination from excessive peptides or trypsin.
More info: www.ssi.shimadzu.com
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|Title Annotation:||READERS SHOWCASE|
|Date:||Sep 1, 2017|
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