Shape of male palpal hook affects female sexual cannibalism in Leucauge mariana (Araneae: Tetragnathidae).
Leucauge mariana (Taczanowski, 1881) females produce mating plugs that totally or partially cover their epigynum (Eberhard & Huber 1998; Aisenberg et al. 2015a). Mating plugs in this species are substances provided by both sexes (Eberhard & Huber 1998). Female participation in plug formation has been suggested as evidence for cryptic female choice in this species due to the fact that females evaluate male sexual performance during courtship and mating, and supply--or not--a substance to the mating plug (Aisenberg & Eberhard 2009). This substance is necessary to form a plug that reduces the probability of future matings, favoring the deposited sperm over sperm of potential male suitors (Eberhard & Huber 1998; Aisenberg & Eberhard 2009). Males of this species possess a chitinized structure located beside the conductor process, the conductor hook (Fig. 1; Mendez & Eberhard (2014)). Its function has been related to plug removal, sperm transfer and it could be involved in stimulation to females during mating (Eberhard & Huber 1998; Mendez & Eberhard 2014).
In a population of L. mariana from Colombia (CO), females showed remarkable differences in sexual behavior compared with a population previously studied in Costa Rica (CR) (Aisenberg & Eberhard 2009; Hernaindez et al. 2018). L. mariana females from CO tended to be more aggressive at the end of the mating and on occasions cannibalized males, a fact which was related to male performance during mating (Hernandez et al. 2018). Previous studies have shown that males' structures such as the conductor hook can play an important role to stabilize the couple during mating (Meindez & Eberhard 2014; Aisenberg et al. 2015a). In this study we tested if the male conductor hook shape in L. mariana from CO affects mating duration, sperm transfer, and female cannibalistic attempts. We predicted that wider male conductor hooks that provide more stability to the genital coupling would determine longer matings, with higher sperm counts and lower frequencies of cannibalistic attempts.
We collected 23 males and 23 sub-adult females of L. mariana between June and August 2015 in San Francisco, Pueblo Viejo, Cundinamarca Province, Colombia (4[degrees]56'30.8"N 74[degrees]16'45.9"W, elevation 1700 m). We carried out 20 male-female pairings, each one inside a terrarium measuring 25 cm long x 10 cm wide x 15 cm high, furnished with small wooden sticks to provide support for the web. The temperature during the trials averaged 23.4[degrees]C([+ or -] 0.2) and relative humidity was 53%. We used males 5 days after their collection from the wild. Trials started when we placed a male on the web at the opposite corner of the terrarium regarding the female. A trial finished when the male or the female remained motionless for 30 min, after 30 min of courtship but without mating, or after mating. All pairs mated on their first exposition. We considered as cannibalistic attempt when the female attacked the male resulting in male injury and/or cannibalization. We deposited voucher specimens at the Museo de Historia Natural de la Universidad de los Andes, Bogota, Colombia.
We determined the shape of the conductor hook tip taking into account a previous study on L. mariana from CR (Mendez & Eberhard 2014). For that purpose, we took photographs of 20 right palpal organs oriented in lateral view and with the same inclination, incidence of light and magnification. We placed each male's hook in the same position using sand and alcohol to stabilize the structure. For each palpal hook, we took three pictures with a Nikon[R] Digital Sight DS Fi1 camera adapted to a stereomicroscope UNITRON Z850. We obtained hook shapes through standard geometric-morphometric methods (Adams et al. 2004). All morphometric measurements were performed on the same day. We placed all the landmarks (three) considering the homology of the structures; the first one corresponded to the tip of the hook, the second and third were located on the base of the hook. This allowed us to place all the semi-landmarks (seven) to follow the shape of the structure. We used tpsDig2 version 2.19 (Rohlf 2015) to place landmarks and semi-landmarks. We placed semi-landmarks because it was not possible to find biologically constant landmarks along the hook. Therefore, we used a grid with the lines consistently separated by 0.01 mm (Fig. 1b). Finally, we used tpsRelw to obtain the uniform and non-uniform shape components, weight matrix, and the centroid size (square root of the sum of squared distances of all the landmarks of an object from their centroid) as a representation of the size component (Rohlf & Slice 1990; Swiderski 2003; Zelditch et al. 2004; Rohlf 2010). The carapace width was measured as a reference for male body size in spiders (Eberhard et al. 1998), and we quantified the amount of the sperm stored in female spermatheca following the modified protocol of Albo et al. (2013).
To determine if hook shape influences mating duration, cannibalism attempts and sperm counts, we ran linear models using lm or glm functions. We visually inspected model assumptions and the appropriate error distribution was chosen accordingly, as well as by comparing the Akaike information criteria (AIC). We used generalized linear models for analyzing sperm counts (Poisson) and cannibalistic attempts (binomial). We performed a multivariate regression between hook centroid size (CS) and shape matrix. To shape the weight matrix (matrix of partial warp scores together with the uniform component for a sample of shapes), we performed a principal component analysis (PCA) and found that the first three components explained 79% of total shape variation (PC1 = 43%, PC2 = 67%, PC3 = 79%). We carried out all statistical analyses in R, version 3.2.3 (R Development Core Team 2015).
We did not find a statistically significant correlation between hook centroid size and male body size (rho = 0.407, df = 1, P = 0.683), meaning that the size of the hook is not explained by body size. Also, we did not find a significant correlation between the hook shape and centroid size (Pillai = 0.872, P = 0.477), meaning that the shape of the hook is not changing with its size. In addition, we did not find any relationship between hook shape and mating duration ([r.sup.2] = 0.074, PC1: P = 0.734; PC2, P = 0.294; PC3: P = 0.98, df = 3). However, when we compared cannibalistic attempts (seven cases) with the hook shape matrix, we obtained a negative relationship with PC2, which represents the base of the hook (Logistic GLM [chi square] = 20.213, df = 1; PC2: P = 0.017, Fig. 2). In this case, hooks with a wider base were related to an increase in cannibalistic attempts. Finally, when we compared sperm counts with hook shape, we did not find any significant relationship between them (Poisson GLM [chi square] = 0.640; PC1, P = 0.865; PC2 P = 0.870; PC3, P = 0.908, df = 3, P = 0.511).
In our study we found that the shape of the hook is related to female cannibalistic attempts. It is probable that a wider base of the tip hook prevents a better coupling of the palp during mating, which could be punished through female attacks (Barrantes et al. 2013) Similarly, in the spider Hemicloea sundevalli Thorell, 1870 (Trochanteriidae, formerly Gnaphosidae), the male tibial retrolateral apophysis is used to anchor the male palp to the epigynum to avoid rotation (Zakharov & Ovtcharenko 2013). It would be interesting to determine if males mechanically stabilize female position during mating depending on hook shape by performing reinforcing behaviors and/or intensifying female stimulation. It is important to highlight that only the base of the hook and not the centroid size was related to cannibalistic attempts. This would mean that the shape of the hook but not its size would have a relationship with cannibalistic attempts. The lack of correlation with male body size suggests that there is no allometric effect on the hook. Though some characteristics under sexual selection can show steep allometries, genital features in general show isometry or shallow allometries depending on the type of selection (Bonduriansky 2007; Eberhard 2009; Cayetano et al. 2011). It would be important to test if longer hooks could play a role in male copulatory courtship in a context of female choice, similarly as has been reported for other arthropod species (Arnaud et al. 2001; Rodriguez et al. 2004; Eberhard 2010; van Lieshout 2011; Dougherty et al. 2015).
Regarding hook shape and mating duration, some reproductive traits like copulation duration depend on the interaction between males and females and the influence of each sex over this (Bretman et al. 2013). For example, in the L. mariana pairs from CR, the duration of copulation is determined by the female and depends on male behaviors such as stimulation by cheliceral setae (Aisenberg et al. 2015b). Hernandez et al. (2018) showed in the L. mariana population from CO that the amount of the sperm in female receptacles is correlated with the rate of long palpal insertions. However, we did not find a relationship between copulation duration, sperm counts and hook shape. Consequently, if the hook provides stability for the mating pair, this would not allow males to extend mating or increase sperm transfer in this species.
L. mariana females from CO perform post-copulatory sexual cannibalism (Hernandez et al. 2018), unlike females from CR (Aisenberg & Eberhard 2009). This could function as a mechanism of cryptic female choice based on male genital traits and sexual performance. Similarly, in Araneus diadematus Clerck, 1757, females tend to cannibalize small males and those that mate but do not transfer sperm (Prenter et al. 2006). Although the conductor hook of L. mariana is not an intromittent structure directly involved with sperm transfer, the shape could play an important role in providing stability and allowing the embolus to be pushed inside the female duct (Eberhard & Huber 1998), as well as in puncturing or removing mating plugs (Aisenberg et al. 2015a). Future studies will analyze other structures involved in genital stability, as well as experiments modifying them and recording the consequences on female and male reproductive success. Moreover, histological procedures performed to male-female pairs during mating could be useful to determine how the male hook is coupled on female genitalia.
The capture of specimens for our study was performed under the permit resolution IDB0359. We thank Luis Hernandez, Eudoro Mora, Valeria Ramirez, Ivan Beltran, Alex Sabogal, and Francisco Quiroz for their help in fieldwork. We thank Alex Sabogal for his collaboration with equipment and Ivam Beltran for his statistical advice. The suggestions of two anonymous reviewers improved the final version of the manuscript. This work was supported by ''Proyecto Semilla'' from the Faculty of Sciences, Universidad de Los Andes. A. Aisenberg acknowledges financial support by PEDECIBA (Programa de Desarrollo de Ciencias Basicas), and SNI (Sistema Nacional de Investigadores, ANII).
Adams, D.C., F.J. Rohlf & D. Slice. 2004. Geometric morphometrics: Ten years of progress following the 'revolution'. Italian Journal Zoology 71:5-16.
Aisenberg, A. & W.G. Eberhard. 2009. Female cooperation in plug formation in a spider: Effects of male copulatory courtship. Behavioral Ecology 20:1236-1241.
Aisenberg, A., G. Barrantes & W.G. Eberhard. 2015a. Postcopulatory sexual selection in two tropical orb-weaving Leucauge spiders. Pp. 79-108. In Cryptic Female Choice in Arthropods: Patterns, Mechanisms and Prospects. (A.V. Peretti & A. Aisenberg, eds.) Springer, Cham, Switzerland.
Aisenberg, A., G. Barrantes & W.G. Eberhard. 2015b. Hairy kisses: Tactile cheliceral courtship affects female mating decisions in Leucauge mariana (Araneae, Tetragnathidae). Behavioral Ecology and Sociobiology 69:313-323.
Albo, M.J., S. Toft & T.B. Bilde. 2013. Sperm storage mediated by cryptic female choice in a gift-giving spider. Proceedings of the Royal Society B: Biological Sciences 280:20131735, doi.org/10.1098/rspb. 2013.1735
Alvarez-Padilla, F. & G. Hormiga. 2011. Morphological and phylogenetic atlas of the orb-weaving spider family Tetragnathidae (Araneae: Araneoidea). Zoological Journal of the Linnean Society 162:713-879.
Arnaud, L., E. Haubruge & M. Gage. 2001. Morphology of Tribolium castaneum male genitalia and its possible role in sperm competition and cryptic female choice. Belgian Journal of Zoology 131:111-115.
Arnqvist, G. 1998. Comparative evidence for the evolution of genitalia by sexual selection. Nature 393:784-786.
Barrantes, G., A. Aisenberg & W.G. Eberhard. 2013. Functional aspects of genital differences in Leucauge argyra and L. mariana (Araneae, Tetragnathidae). Journal of Arachnology 41:59-69.
Bonduriansky, R. 2007. Review Sexual selection and allometry: A critical reappraisal of the evidence and ideas. Evolution 61:838-849.
Brennan, P.L.R. & R.O. Prum. 2015. Mechanisms and evidence of genital coevolution: The roles of natural selection, mate choice, and sexual conflict. Cold Spring Harbor Perspectives in Biology 7:a017749.
Bretman, A., J.D. Westmancoat & T. Chapman. 2013. Male control of mating duration following exposure to rivals in fruitflies. Journal of Insect Physiology 59:824-827.
Cayetano, L., A. Maklakov, R. Brooks & R. Bonduriansky. 2011. Evolution of male and female genitalia following release from sexual selection. Evolution 65:2171-2183.
Dougherty, L.R., I.A. Rahman, E.R. Burdfield-Steel, E.V. Greenway & D.M. Shuker. 2015. Experimental reduction of intromittent organ length reduces male reproductive success in a bug. Proceedings of the Royal Society B: Biological Sciences 282:20150724.
Eberhard, W.G. 1985. Sexual Selection and Animal Genitalia. Harvard University Press, Cambridge, MA.
Eberhard, W.G. 1996. Female Control: Sexual Selection by Cryptic Female Choice. Princeton University Press, Princeton, NJ.
Eberhard, W.G. 2009. Static allometry and animal genitalia. Evolution 63:48-66.
Eberhard, W.G. 2010. Evolution of genitalia: theories, evidence, and new directions. Genetica 138:5-18.
Eberhard, W.G. & B.A. Huber. 1998. Courtship, copulation and sperm transfer in Leucauge mariana (Araneae, Tetragnathidae) with implications for higher classification. Journal of Arachnology 26:342-368.
Eberhard, W.G., B. Huber, R.L. Rodriguez, R. Briceno, I. Salas & V. Rodriguez. 1998. One size fits all? Relationships between the size and degree of variation in genitalia and other body parts in twenty species of insects and spiders. Evolution 52:415-431.
Hernandez, L., A. Aisenberg & J. Molina. 2018. Mating plugs and sexual cannibalism in the Colombian orb-web spider Leucauge mariana. Ethology 2017:1-13.
Johnstone, R.A. 1995. Sexual selection, honest advertisement and the handicap principle: reviewing the evidence. Biological Reviews of the Cambridge Philosophical Society 70:1-65.
Mendez, V. & W.G. Eberhard. 2014. Removal of genital plugs and insemination by males with normal and experimentally modified palps in Leucauge mariana (Araneae, Tetragnathidae). Journal of Arachnology 42:284-292.
Parker, G.A. 1970. Sperm competition and its evolutionary consequences. Biological Reviews 45:525-567.
Prenter, J., C. R. MacNeil & W. Elwood. 2006. Sexual cannibalism and mate choice. Animal Behaviour 71:481-490.
R Development Core Team (2015). R: A language and environment for statistical computing. Version 3.2.3. Online at http://www.R-project.org
Rodriguez, V., D.M. Windsor & W.G. Eberhard. 2004. Tortoise beetle genitalia and demonstrations of a sexually selected advantage for flagellum length in Chelymorpha alternans (Chrysomelidae, Cassidini, Stolaini). Pp. 739-748. In Developments in the Biology of the Chrysomelidae. (P. Jolivet., J.A. Santiago-Blay & M. Schmitt, eds.), SPB Academic Publishing, The Hague.
Rohlf, F.J. 2010. Relative warps version 1.49. Online at http://life.bio.sunysb.edu/morph/index.html
Rohlf, F.J. 2015. tpsDig2 version 2.19. Available at http://life.bio.sunysb.edu/morph/index.html
Rohlf, F.J. & D. Slice. 1990. Extension of the Procrustes method for the optimal superimposition of landmarks. Systematic Zoology 39:40-59.
Swiderski, D.L. 2003. Separating size from allometry: analysis of lower jaw morphology in the fox squirrel, Sciurus niger. Journal of Mammalogy 84:861-876.
Van Lieshout, E. 2011. Male genital length and mating status differentially affect mating behaviour in an earwig. Behavioral Ecology and Sociobiology 65:149-156.
World Spider Catalog. 2018. World Spider Catalog. Online at http://www.wsc.nmbe.ch/ Last accessed 16 January 2018.
Zakharov, B. & V. Ovtcharenko. 2013. Structure of genital organs and mating process of the spider Hemicloea sundevalli Thorell, 1870 (Araneae: Gnaphosidae). Entomologica Americana 119:47-56.
Zelditch, M.L., D.L, Swidersky, H.D. Sheets & W.L. Fink. 2004. Geometric Morphometrics for Biologists: A Primer. Elsevier Academic Press. New York.
Linda Hernandez (1), Jorge Molina (1) and Anita Aisenberg (2): (1) Centro de Investigaciones en Microbiologia y Parasitologia Tropical - CIMPAT, Bogota Universidad de los Andes, Bogota, Colombia; E-mail: email@example.com; (2) Departamento de Ecologia y Biologia Evolutiva, Instituto de Investigaciones Biologicas Clemente Estable, Montevideo, Uruguay
Manuscript received 26 June 2018, revised 25 January 2019.
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|Title Annotation:||SHORT COMMUNICATION|
|Author:||Hernandez, Linda; Molina, Jorge; Aisenberg, Anita|
|Publication:||The Journal of Arachnology|
|Date:||May 1, 2019|
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