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Session 8 medicine, health & physiology Nitschke Rm 3004 T.B.A., presiding.

9:00 AM

DIFFERENTIAL EXPRESSION OF SIX SRY ISOFORMS IN SKELETAL MUSCLE FROM TWO STRAINS OF Rattus norvegicus. Lauren A. Playl, Jeff Dunmire, Monte Turner, Amy Milsted, The University of Akron, Dept of Biology, 302 Buchtel Commons, Akron OH 44325-3908.

Sry, a Y-linked transcription factor expressed in male genital ridge embryonic cells, directs testis development. Sry transcripts have been reported in adult mouse brain and human testis, prostate, and prostate tumors. The rat genome contains six Sry genes. Tissue and strain specific differences in Sry expression profiles have been reported in testis, adrenal, and kidney of borderline hypertensive SHR/y and normotensive WKY rats. It has been proposed that rat Sry 1 confers elevated blood pressure in SHR/y rats compared to WKY controls. To begin to study Sry expression and function in other adult tissues, we hypothesized that Sry is expressed in adult male rat skeletal muscle with strain specific differences in the gene or isoform expression profiles. Skeletal muscle RNA samples from 15-20 week old WKY and SHR/y rats were reverse transcribed to generate cDNA. Sry cDNA was amplified with fluorescent-tagged primers and relative proportions of Sry isoforms determined by fragment analysis. Each sample (n=2 per strain) was run in duplicate and results individually averaged for each isoform. WKY skeletal muscle expressed Sry 2 (91.1 [+ or -] 8.6%) and Sry 1 (8.2 [+ or -] 8.2%). SHR/y skeletal muscle expressed Sry 2 (55.3 [+ or -] 38.9%), Sry 1 (12.8 [+ or -] 12.8%), Sry 3 (28.9 [+ or -] 21.6%), and combined Sry 3B/ 3B1 (3.84 [+ or -] 3.84%). Neither strain expressed Sry 3C. Strain specific differences in Sry expression profiles existed for all Sry isoforms except Sry 1. Differences in expression profiles of the Sry isoforms are consistent with differences in regulation of these genes in SHR/y versus WKY rats.

9:15 AM TOY CHOICE IN AN AUTISTIC FEMALE Lauren R. Meyer Denison University, 8342 Slayter Union, Granville, OH 43023.

Previous studies have demonstrated that children with autism tend to play with human-like toys less often than their typically developing peers. Since autism affects males four times as often as females, these previous studies may be showing that the predominantly male subjects used were exhibiting gender-related preferences not influenced by autism. This IRB-approved investigation studied an autistic female to determine whether she differed in toy choice from a typically developing female peer (both aged five). It was hypothesized that the autistic subject would choose dolls and figurines less often than her normally developing peer. Three sessions, each consisting of five trials each lasting up to 7 minutes, were conducted with each child. They were presented with two toys, one "human-like" figurine, and one "non-humanlike" toy such as a car or manipulative. The autistic girl chose to play with the human-like toys 2/15 times while the typically developing girl did 6/15. When human toys were chosen, the autistic girl spent an average of 82 seconds in engaged play and the typically developing girl spent 139 seconds. The small number of trials renders the difference in toy choice and engagement time statistically insignificant. The autistic girl played longer with the "other" toys (average of 165 seconds) than with the human-like toys (82 seconds). These trends are consistent with previous studies and suggest that autism itself, not merely gender, may be the cause of observed differences in play.

9:30 AM POSTPRANDIAL TEMPERATURE SELECTION IN NOTOPHTHALAMUS VIRIDESCENS. Kimberly Ann Gieras,, Spiro Mavroidis,, Defiance College, 701 N. Clinton St. Defiance OH 43512.

Body temperature in animals greatly influences biochemical processes, especially in ectoderms, as they are unable to generate their own body heat and must rely on their immediate environment for thermoregulation. This study will focus on Notophthalamus viridescens, the Eastern red-spotted newt, which is native to the northern United States and Canada and leads an aquatic lifestyle as an adult. The purpose of this study is to compare the animal's preferred postprandial body temperature to that of its preferred normal temperature. It is hypothesized that the newt will move to a higher temperature during digestion. A Plexiglas arena has been constructed with separate quadrants, each set at a specific water temperature (15, 18, 21 and 24 [degrees]C) and easily accessible to the animals. Aquarium heaters, filters and temperature probes will be used for regulating and monitoring temperature. It is assumed that due to the small size of N. viridescens, water temperature will be equal to the animal's body temperature. The newts will be led once each week, with the mass of each animal being measured before and after feeding to calculate consumption. Data collection will begin in mid-November 2007 and continue for 6 weeks. It is be expected that the newts will prefer a higher postprandial temperature compared to that found during fasting. Also, it is expected that the animals will follow a daily routine of temperature regulation on non-feeding days, choosing a higher temperature before 9:00 in the morning and a lower temperature after 6:00 in the evening.


This project investigated the effectiveness of cleaning procedures used in the fitness, weight, and locker rooms of Ohio Northern University (ONU) athletic facilities by monitoring bacterial contamination of fomites. Additional tests were used to evaluate the presence of a common skin microbe, Staphylococcus spp., and in particular, Methicillin-resistant Staphylococcus aureus (MRSA). Using swabs moistened in sterile nutrient broth, samples were collected from different fomites (N=23) in the facilities18 hours after scheduled cleaning, once monthly from August- November, 2005. Samples were transported using Stuart medium. Following vortex extraction in 2 ml of sterile saline, each was streaked for isolation on blood agar (growth control) and on Mannitol Salt Agar (MSA) and incubated at 37[degrees]C for 24-48 hours. Overall microbial contamination was quantified via colony count. Yellow colonies on the MSA were tested for coagulase activity using a latex agglutination assay. Gram positive Staphylococci colonies with a positive coagulase test (17) were inoculated onto Mueller-Hinton agar containing 6 ig/ml Oxacillin and incubated for 24 hours at 37[degrees] C to determine their sensitivity to the antibiotic. No growth was recorded indicating the S. aureus collected was not MRSA. Uncleaned facilities demonstrated distinct trends of increased overall microbial contamination, and specifically S. aureus growth, as compared to sites that received stringent, daily cleaning. The data suggest a correlation between effective cleaning and decreased microbial contamination.

10:00 AM EFFECTS OF THERAPY AND EXERCISE ON DELAYED ONSET MUSCLE SORENESS Alexandra L. Miller Ohio Northern University 402 W College Ave Unit# 3412 Ada OH 45810.

Delayed onset muscle soreness (DOMS) is the pain and discomfort that occurs after unaccustomed eccentric activity, and it can interfere with one's daily activities. The purpose of this study was to investigate whether twenty minutes of thermotherapy, cryotherapy, or moderate-intensity cardiovascular exercise could effectively reduce the severity of delayed-onset muscle soreness. To induce DOMS, twenty four untrained females performed three sets of 10 maximal eccentric repetitions on a Paramount AP-2000[TM] leg extension machine using only one leg. The subjects were randomly assigned to a thermotherapy, cryotherapy, moderate-intensity cardiovascular exercise, or a no therapy (control) group. Treatments were administered for 20 minutes 24 hours after the eccentric exercises were completed. Range of motion and muscle soreness were measured pre, 24, and 48 hours post-exercise. ANOVA test (a=0.05) demonstrated that baseline range of motion and 24 hour post-exercise pain levels were not significantly different among groups. Paired two sample means t-Tests (a=0.05) showed that cryotherapy (p=0.034), thermotherapy (p=0.021), and exercise (p=0.004) all significantly improved knee flexion. However, only thermotherapy and moderate-intensity cardiovascular exercise significantly improved flexion and reduced muscle soreness (p=0.024 and p=0.008, respectively). These results indicate that both thermotherapy and moderate-intensity cardiovascular exercise effectively alleviate DOMS.

10:15 AM

BLADDER DYSFUNCTION. De Nguyen, No. 0140, 9205 Telfer Run, Orlando FL 32817.

The purpose of this paper is to present the possibility of a bladder dysfunction. Patrick C. Walsh found that many men bad all the symptoms of a benign prostatic hypertrophy without an enlarged prostate. Three male patients of the Floyd Medical Clinic with hypertension exhibited urinary frequency, urgency, and urge incontinence at the age of 50 years. The examination of their prostate was normal. Their laboratory test results were normal including a PSA below 3 ng/ml. Their blood pressure of 145/92 mmHg was treated with Nifedipine XL 30 mg daily. They returned a week later for follow up. Surprisingly, their urinary irritative symptoms were controlled as well as their blood pressure. They were followed up to 4 years with yearly PSA levels below 4 ng/ml. These cases suggested the possibility of age-related bladder dysfunction which is probably secondary to atherosclerosis. A hypothesis of local arterial stenosis induces a metabolic acidosis that triggers uncontrolled and erratic muscle contractions of the bladder leading to clinical symptoms. Vasodilator agents release hypoxia in the early stage. A better study with much larger sample size of patients is needed with angiographic investigation and urinary flow measurement to clarify the hypothesis.
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Publication:The Ohio Journal of Science
Geographic Code:1U3OH
Date:Mar 1, 2008
Previous Article:Session 7 ecology Nitshcke Rm 2004 T.B.A., presiding.
Next Article:Poster session: Nitschke Hall 9:00-11:00 AM.

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