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Seed germination and seedling growth of two Pseudobombax species (Malvaceae) with contrasting habitats from Brazilian Cerrado.

Seed germination patterns differ in the same family, genus and even for the same species (Baskin & Baskin 1998). Inside the Bombacaceae, now the subfamily Bombacoideae, Malvaceae sensu lato (APG 2003), there are species with dormant and non-dormant seeds (Vazquez-Yanes 1974, Joly & Crawford 1983, Danthu et al. 1995), but information about seed germination patterns is not available yet for most taxa of this group.

Germination within the large Neotropical genus Pseudobombax is still poorly studied and the few published works are restricted to the germinability assessment, and sometimes to the speed of the process, based on the first and the last seed germinated in experimental conditions (Souza & Valio 2001, Souza-Silva et al. 2001, Zamith & Scarano 2004, Wittmann et al. 2007, Lopes et al. 2008). Other studies for the family focused on the ratio between dry mass of roots and shoots or on seedling growing under different light and soil conditions, without records for Pseudobombax (Moreira & Klink 2000, Scalon et al. 2003).

Among the species of Bombacoideae from cerrado, P. longiflorum and P. tomentosum are species that occur in open fire-prone plant formations, but also at the edge or inside gallery, deciduous and mesophyllous forests (Gribel 1988, Silva & Scariot 2004). P. longiflorum is much more common in open savanna formations and has hanging bat pollinated flowers (Coelho & Marinho Filho 2002). P. tomentosum is much more common in forest habitats where its sturdy flowers depend on non-flying mammals passing from crown to crown for pollination (Gribel 1988). Except from the differences in habitat, the species are similar in relation to the distribution, phenology and seed dispersion period (Lorenzi 1992, Silva Junior et al. 2005). The absence of exclusive habitats between the two species reflect the presence of physiognomic gradients and transitions among savanna and forest areas in the Cerrado Biome (Ribeiro & Walter 1998). The water stress and the occurrence of seasonal fire in the Cerrado biome select species with ability to resprout after these events, leading to the allocation of available resources to the roots or other underground structures in detriment of the shoots in open formation species while forest species show allocation to aerial parts in detriment of the roots (Hoffmann & Franco 2003). Inside mesophyllous forest and gallery forest formations, light is the limiting factor but habitat shows less oscillations in water regime and temperature, favoring fast and synchronized seed germination whenever gaps or leaf fall cycles improve seedling survival chances as discussed by Garwood (1983). Meanwhile, in the savanna habitats, the limiting factors are water and nutrients, and seed germination spread in time, and even dormancy, may favor seedling survival (Moreira & Klink 2000, Hoffmann & Franco 2003, Oliveira 2008, vieira et al. 2008).

In this context, vicariant species as the ones studied here provide the opportunity to see how habitat specialization would be reflected in germination and seedling growth (Hoffmann & Franco 2003). The objective of this paper was to test, on the one hand, if seed germination of P. longiflorum would be more asynchronous and seedling mass allocated predominantly in the underground structures, favoring seedling survival in the open savanna areas. On the other hand, for P. tomentosum, typical of forest formation with less water stress, with an expected more synchronized germination process and seedlings would have more mass allocated to the stems, favoring light absorption and continuous growth.

MATERIAL AND METHODS

Studied species: Pseudobombax longiflorum (Mart. & Zucc.) Robyns (Bombacoideae, Malvaceae) is found in most Cerrado areas. It is fairly common in Minas Gerais and down to the North of Parana State. It is a deciduous tree, which occurs sparsely in open Cerrado areas (Lorenzi 1992). Flowering occurs from July to November and fruits mature during the next dry season, from July to November (Silva Junior et al. 2005). P. tomentosum (Mart. & Zucc.) Robyns (Bombacoideae, Malvaceae) is usually a larger tree, found in Cerradao (dense cerrado with almost closed woodland) and Cerrado (closed vegetation dominated by trees and shrubs with herbaceous vegetation between them), but mainly in the edges and sometimes inside mesophyllous and gallery forests. It occurs in most Cerrado region down to Southern areas of Sao Paulo and Mato Grosso do Sul. It flowers from July to August and the fruits mature from August to October. It is a deciduous and heliophyllous plant, also appearing less frequently in open vegetation, usually on sandy or humic clay soils, but also on better drained oxisoils common in the Cerrado region (Lorenzi 1992, P.E. Oliveira, pers. observ.). The voucher specimens can be found in Herbarium Uberlandense.

Studied area: About 200 seeds of both species were collected in the Cerrado areas, Neotropical savannas region in Central Brazil (sensu Ribeiro & Walter 1998). The seeds of P. tomentosum were collected in forest areas, near to Uberlandia city, Minas Gerais state (19[degress]07' W--48[degress]22' S) and the seeds of P. longiflorum were collected in adjacent open pasture and areas along the BR050 highway (17[degress]06' W--47[degress]45' S), between Uberlandia and Brasilia. Germination and seedling growth experiments were carried out at the Campus of the Federal University of Uberlandia. The region climate is characterized as Aw according to the Koppen scale (Koppen 1948), tropical humid climate with a dry winter (April to September) and rainy summer (October to March) (Rosa et al. 1991).

Seed germination: Mature fruits of both species were collected in September and October 2003. The fruits were collected directly from the trees and placed to dry at room temperature in order to facilitate the extraction of the seeds. Seeds were stored in paper bags at room temperature (between 25 and 30[degress]C), in a dry chamber containing silica gel with humidity indicator, and kept until the installation of the experiment, 30 days later. The seeds were sown on fine vermiculite (expansion volume of 0.1[m.sup.3]), inside transparent germination boxes, moistened as necessary with distilled water. Boxes were kept in a germination chamber (Seedburo Company, model MDG2000) under continuous light (mean = 11.90, SD = 6.52[micro]mol/ [m.sup.2]/s of photosynthetically active radiation), at 25[degress]C. The experimental units were randomly distributed in the germination chamber, four replicates for each species with 25 seeds per replicate. The seed sample size was somewhat limited as a consequence of the high level of predation of fruits and seeds during the collection year. Although seed samples used for the experiments were rather small, the data was still sufficient for consistent statistical tests.

The number of germinated seeds was observed daily and protrusion of the radicle or any part of the embryo was used as germination criterion. Germinability (G), represented by the percentage of germination in the experimental conditions (Labouriau 1983), mean germination time (MGT) ([bar.t], Labouriau 1970), germination time to 50% germination (GT50), germination time of the first germinated seed (GTFS), germination time of the last germinated seed (GTLS), coefficient of variation of the germination time (CvGT) ([Cv.sub.t], Ranal & Santana 2006), mean germination rate (MGR) ([bar.v], Labouriau 1970), uncertainty of germination (UG) (U, Labouriau & valadares 1976) and synchronization index of germination (ZG) (Z, Ranal & Santana 2006 adapted from Primack 1980) were used to describe the germination process. Further details on mathematical expressions, authorship, intermediate calculus, the sense and the applications of these germination measurements can be found in Ranal & Santana (2006) and Ranal et al. (2009). All germinated seeds were checked for the presence of more than one embryo per seed, known as polyembryony and common in Bombacoideae (Mendes-Rodrigues et al. 2005).

Seedling morphology and height: Seeds germinated in laboratory conditions were transplanted to black plastic bags (30cm high per 13cm diameter), filled with previously sieved open Cerrado oxisol. Seedlings were kept in a greenhouse covered with black plastic net with 50% shading and moistened when necessary. Starting from emergence date, periodic measurements of the maximum height of each plant, considered from ground to the apex of the last leaf were carried out (Fig. 1 H-1). The data were adjusted to a linear regression model, as a function of the days from emergence. Some 20 P. longiflorum seedlings were evaluated up to 67 days after emergence and 15 P. tomentosum seedlings up to 63 days after emergence, when initial growth appeared to stabilize (seedlings showed no further increment in height). The seedling functional morphology was classified based on Miquel (1987). These seedlings were kept for nine months under the same conditions to asses a biomass allocation evaluation, as described below.

Biomass allocation: Seedlings for biomass evaluation were analyzed nine months after sowing, before foliar abscission started. Seven plants of each species were removed from the soil and analyzed for the leaf number per plant, length of the shoot (considered from the soil to the apex of the last leaf) (Fig. 1 H-1), height of the apical meristem (from soil to the apical meristem) (Fig. 1 H-2), height of insertion of the first leaf from soil (Fig. 1 H-3), diameter at the shoot base, the largest diameter of the underground structure (Fig. 1 H-4) and the length of the underground structure (Fig. 1 H-5). The seedlings were divided into aerial (stems and leaves) and underground parts (mostly roots), and dried to constant mass at 70[degress]C (eight days). The dry mass ratio between underground and aerial parts and between underground and total seedling dry mass were calculated (Hunt 1990). Non-quantitative observations continued up to 18 months after sowing.

[FIGURE 1 OMITTED]

Germination measurements and seedling biometry data were evaluated for normality with the Shapiro-Wilk test. Data with normal distribution were compared using Student's t-test (all seedling biometry measurements, except underground structure length) and data without normal distribution were compared by Mann-Whitney test (all germination measurements, plus underground structure length). The relative frequency of seed germination was compared between species using the Kolmogorov-Smirnov test. The seedling height collected during the first 70 days was adjusted to a simple linear regression model as a function of the time from emergence and the significance of the model tested with ANOvA. The slope and intercept of the linear regression were compared between species using Student's t-test. For all the statistical analysis 0.05 of significance was adopted and procedures were based on Sokal & Rolf (1995).

RESULTS

Seed germination: Seeds of P. longiflorum and P. tomentosum did not present significant differences in germinability, mean germination time and mean germination rate (Table 1). On the other hand, seeds of P. tomentosum presented greater homogeneity (lower value of CvGT) and higher synchrony of germination (lower value of UG and higher value of ZG) than P. longiflorum. The first germination was registered for P. longiflorum four days after the beginning of the experiment and the last germination occurred after 20 days. For P. tomentosum germination started after seven days and continued up to the 18th day after sowing (Fig. 2). The distribution of the relative frequency of germination differs between species (D = 0.1980, p<0.05) and demonstrated that in P. longiflorum, this process was more spread out through time, with some peaks of germination in days 11, 13, 14 and 16, while in P. tomentosum, germination was less spread out in time and a strong germination peak occurred at the 11th day after sowing (Fig. 2). After 20 days, the remaining seeds were already degenerating and no further germination occurred. The two species presented only one embryo per seed and no polyembryony was recorded.

[FIGURE 2 OMITTED]

Seedling morphology and height: The seedlings of both species presented photosynthetically active cotyledons exposed above ground (Fig. 1 A-C) and were classified as phanero-epigeal-foliaceous (PEF sensu Miquel 1987). In classical PEF type the cotyledons are suspended from the ground by elongation of hypocotyl, while in these two species of Pseudobombax the elongation is caused by elongation of cotyledon petiole (Fig. 1 A). Although cotyledons are hold above ground, their fixation point remains below ground (Fig. 1 A-C). The seedling height of both species was well adjusted to a simple linear regression model ([r.sup.2] = 0.8091, [F.sub.1,105] = 445.02, p<0.0001 for P. longiflorum and [r.sup.2] = 0.8087, [F.sub.1,105] = 393.21, p<0.0001 for P. tomentosum) (Fig. 3A, 3B). The two species showed no significant differences between the slopes of the equation (t = 0.136, d.f. = 198, p>0.05), nor between intercepts (t = 0.832, d.f. = 199, p>0.05), presenting basically the same pattern of continuous growth during the first 70 days.

[FIGURE 3 OMITTED]

Biomass allocation of dry mass: Both species presented an underground structure (mostly roots) which represented about 80% of the total mass of seedlings (Fig. 1 E-F). Among the analyzed characteristics, the height of the apical meristem, diameter and the dry mass of underground structure, shoot and whole seedling dry mass were statistically higher in P. longiflorum in comparison with P. tomentosum (Table 2). For the other characteristics there were no significant differences. Both species cessed growth between 10 and 12 months, followed by leaf loss and resprout after the cool dry period (Fig. 1 D-G).

DISCUSSION

Both species of Pseudobombax presented high germinability and similar growth patterns, but there were some differences in germination time and synchrony, which may be associated to their preferential habitat. The high germinability of the seeds of both Pseudobombax studied species is similar to the one observed in other Bombacoideae (Melo et al. 1979, Sousa-Silva et al. 2001, Scalon et al. 2003, Zamith & Scarano 2004, Fanti & Perez 2005, Maia et al. 2005, Wittmann et al. 2007, Lopes et al. 2008, Zamora-Cornelio et al. 2010), and contrast with lower germinability recorded for other species of Pseudobombax (Sanchez & Zepeda 2004, Sautu et al. 2006). This also indicates the absence of marked dormancy in Pseudobombax, although species with mechanical dormancy occur in Bombacoideae (Danthu et al. 1995, Barbosa et al. 2004, Pinto et al. 2004).

The germination process of both species was relatively slower; with mean germination times higher than the 5.1 to 7.1 days recorded for other Bombacoideae (Souza & Valio 2001, Ranal et al. 2010), but was similar to seedling emergence times described for the group (Mendes-Rodrigues et al. 2005). The germination time to 50% of germination (GT50) was also higher than the 5 to 7 days recorded for other species in the group (Maia et al. 2005, Zamora-Cornelio et al. 2010).

The duration of germination process was distributed along 4.50 to 18.25 days in the studied species (Germination time of the first seed GTFS and of the last seed-GTLS), which is inside the ample variation recorded for species in the group, which ranged from 3 (Zamora-Cornelio et al. 2010) to 19 days (Sautu et al. 2006) to GTFS and from 6 (Zamora-Cornelio et al. 2010) to 284 days (Sautu et al. 2006) to GTLS.

The gradual germination process for both studied species of Pseudobombax can be considered a type of relative dormancy (sensu Labouriau 1983). It is not a mechanical dormancy as observed for Ochroma pyramidale (Cav. ex Lam.) Urb. (Zamora-Cornelio et al. 2010), but it results in a germination process spread through time in opposition to a single germination peak observed in e.g. Ceiba speciosa (A.St.-Hil., A.Juss. & Cambess.) Ravenna (Ranal et al. 2010). This strategy of spreading the germination through time is present in other species occurring in the Cerrado region (Carvalho et al. 2005, Pereira et al. 2009, Ranal et al. 2010, Mendes-Rodrigues et al. 2010).

P. tomentosum showed significantly higher synchronization of germination than that observed for P. longiflorum seeds. The more asynchronous and heterogeneous germination observed for P. longiflorum seeds would increase survival ability at the beginning of the rains, which may vary in intensity and frequency in the Cerrado region (Oliveira 2008).

The seedlings of the two species of Pseudobombax differed from others species within the subfamily as Bombacopsis glabra (Pasq.) A. Robyns (Baker 1960) and Eriotheca pubescens Schott & Endl. (Mendes-Rodrigues et al. 2005) which are polyembrionic. The functional morphology type of seedlings of P. longiflorum and P. tomentosum differed from P. ellipticum (Kunth) Dugand and P. munguba (Mart. & Zucc.) Dugand, which present the cotyledons suspended by hypocotyl elongation (Sanchez & Zepeda 2004, Maia et al. 2005), a pattern commonly observed in classical PEF type (Ressel et al. 2004).

P. longiflorum and P. tomentosum presented similar growth pattern during the first 70 days. This trend of linear initial growth has been also observed for other Bombacoideae such as Ceiba pentandra (L.) Gaertn. (Pedroso & varela 1995) and B. glabra (Scalon et al. 2003). The leaf fall and resprout after the cooler part of the dry season were not previously recorded for Pseudobombax, but this brevideciduous leafing behavior is common among seedlings of other Cerrado species (Oliveira & Silva 1993, Oliveira 2008).

Larger seedlings observed in P. longiflorum in relation to P. tomentosum could also be viewed as a way to increase chances of survival, since open Cerrado plants have a limited growing season (Oliveira 2008). Nevertheless, both species presented seasonal growth and dry matter allocation ratios similar to other Cerrado trees (Oliveira & Silva 1993, Moreira & Klink 2000). Despite their difference in predominant habitat, the two species of Pseudobombax allocated most dry mass to underground structures in detriment of the shoots. This root biased allocation would allow greater resistance to water and temperature stress during the dry period in open Cerrado plant formations, but would hinder competition and growth in forest environments (Hoffmann & Franco 2003, vieira et al. 2008). The preferential investment to underground structures has been also recorded for other species from open Cerrado formations (Moreira & Klink 2000, Hoffmann & Franco 2003), including some species of Bombacoideae (Moreira & Klink 2000, Ronquim et al. 2003, Ressel et al. 2004). But even for vicariant pairs of species restricted to either the savanna or forest, the physiological and developmental features were not easy to contrast (Hoffmann & Franco 2003). It is important to notice here that the differences in seedling size may be a result of a better adjustment of P. longiflorum seeds to the Cerrado soil used in the experiment.

Although the two Pseudobombax species differ in synchrony of germination and in some aspects of growth pattern, those differences are not clearcut and may be explained by the distribution overlap between species. New and more refined experiments will be necessary to see whether these differences, despite being significant, would really favour habitat specialization in the studied species.

ACKNOWLEDGMENTS

Thanks to Conselho Nacional de Desenvolvimento Cientifico e Tecnologico--CNPq, and Fundacao de Amparo a Pesquisa do Estado de Minas Gerais--FAPEMIG for the research grant to the first author and the Denise Garcia de Santana for help in some of the statistical analysis. To Glauco Machado and Felipe Wanderley Amorim for the reading of the first versions of the manuscript and to Francielle Paulina de Araujo for the revision of the resumen. The results are part of the MSc. dissertation of the first author in the Post-Graduate Program of Ecology and Conservation of Natural Resources--Universidade Federal de Uberlandia.

Received 22-XI-2010.

Corrected 07-IV-2011.

Accepted 09-V-2011.

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Clesnan Mendes-Rodrigues, Paulo Eugenio Oliveira & Marli Aparecida Ranal

Programa de Pos-graduacao em Ecologia e Conservacao de Recursos Naturais, Instituto de Biologia, Universidade Federal de Uberlandia, Caixa Postal 593, 38400-902 Uberlandia, Minas Gerais, Brazil; clesnan@hotmail.com,

poliveira@ufu.br, ranal@ufu.br
TABLE 1
Mean germination measurements ([+ or -]SD) of P. longiflorum and
P. tomentosum (Bombacoideae, Malvaceae) seeds

Measurement (unity)(1)    P. longiflorum         P. tomentosum
G (%)                   99.00 [+ or -] 2.00   97.00 [+ or -] 2.00
MGT (day)               12.17 [+ or -] 0.32   11.68 [+ or -] 0.48
GT50 (day)              13.25 [+ or -] 0.50   13.25 [+ or -] 0.50
GTFS (day)              4.50 [+ or -] 1.00    7.75 [+ or -] 0.50
GTLS (day)              18.25 [+ or -] 1.26   16.75 [+ or -] 0.96
cvgt (%)                30.17 [+ or -] 1.78   22.25 [+ or -] 4.83
MGR ([day.sup.-1])      0.08 [+ or -] 0.02    0.09 [+ or -] 0.003
UG (bit)                3.29 [+ or -] 0.09    2.81 [+ or -] 0.24
ZG                      0.08 [+ or -] 0.004   0.14 [+ or -] 0.04

Measurement (unity)(1)    U (2)       p (3)
G (%)                     4.00       0.2482
MGT (day)                 4.30       0.1489
GT50 (day)                8.00       1.0000
GTFS (day)                0.00       0.0209
GTLS (day)                2.50       0.1124
cvgt (%)                  4.00       0.0209
MGR ([day.sup.-1])        4.30       0.1489
UG (bit)                  4.00       0.0209
ZG                        4.00       0.0209

(1.) CvGT = coefficient of variation of the germination time,
G = germinability, MGT = mean germination time, MGR = mean
germination rate, GT50 = germination time to 50% germination,
GTFS = germination time of the first seed, GTLS = germination
time of the last seed, UG = uncertainty of germination,
ZG = synchronization index of germination.

(2.) U = statistics of the Mann Whitney test.

(3.) p = probability.

TABLE 2
Mean of biometry and allocation of dry matter
in seedlings ([+ or -]SD) of P. longiflorum
and P. tomentosum (Bombacoideae,
Malvaceae), cultivated by nine months under
50% of natural light in Uberlandia, Minas
Gerais state, Brazil

 Measurement (unity)        P. longiflorum

Number of leaves           4.00[+ or -]0.67
Seedling height (mm)     128.29[+ or -]13.41
Apical meristem           59.07[+ or -]9.33
  height (mm)
Shoot diameter (mm)        6.56[+ or -]0.98
Underground structure    113.95[+ or -]18.23
  length (mm)
Underground structure     24.54[+ or -]1.14
  diameter (mm)
Height of the first       29.30[+ or -]11.44
  leaf insertion (mm)
Aerial dry mass (mg)    1 179.76[+ or -]156.52
Underground dry mass    6 145.10[+ or -]277.16
  (mg)
Seedling dry mass       7 324.86[+ or -]331.90
  (mg)
Underground/aerial         5.09[+ or -]0.64
  dry mass ratio
Aerial/seedling mass       0.16[+ or -]0.02
  ratio

 Measurement (unity)         P. tomentosum        [Statistics.sup.1]

Number of leaves           4.28[+ or -]0.90            t = 0.60
Seedling height (mm)      118.30[+ or -]17.79          t = 1.19
Apical meristem            41.06[+ or -]8.49           t = 3.78
  height (mm)
Shoot diameter (mm)        6.94[+ or -]1.27            t = 0.63
Underground structure     109.19[+ or -]13.51         U = 29.00
  length (mm)
Underground structure      20.60[+ or -]1.33           t = 5.96
  diameter (mm)
Height of the first        23.07[+ or -]6.98           t = 1.23
  leaf insertion (mm)
Aerial dry mass (mg)     981.67[+ or -]181.15          t = 2.19
Underground dry mass    4 310.81[+ or -]1142.06        t = 4.13
  (mg)
Seedling dry mass       5 292.48[+ or -]1200.35        t = 4.32
  (mg)
Underground/aerial         4.27[+ or -]1.06            t = 1.76
  dry mass ratio
Aerial/seedling mass       0.20[+ or -]0.001           t = 1.98
  ratio

 Measurement (unity)    [p.sup.2]

Number of leaves         0.5577
Seedling height (mm)     0.2585
Apical meristem          0.0026
  height (mm)
Shoot diameter (mm)      0.5373
Underground structure    0.6200
  length (mm)
Underground structure    0.0001
  diameter (mm)
Height of the first      0.2425
  leaf insertion (mm)
Aerial dry mass (mg)     0.0490
Underground dry mass     0.0061
  (mg)
Seedling dry mass        0.0050
  (mg)
Underground/aerial       0.1030
  dry mass ratio
Aerial/seedling mass     0.0707
  ratio

1. t = statistics of the t "Student" test,
U = statistics of the Mann Whitney test.

2. p = probability.
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Title Annotation:texto en ingles
Author:Mendes-Rodrigues, Clesnan; Oliveira, Paulo Eugenio; Ranal, Marli Aparecida
Publication:Revista de Biologia Tropical
Date:Dec 1, 2011
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