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Sectioned Images of a Cat Head to Contribute to Learning of its Sectional Anatomy/ Imagenes Seccionadas de una Cabeza de Gato para Contribuir al Conocimiento de su Anatomia Seccional.

INTRODUCTION

Unlike computed tomographs (CTs) and magnetic resonance images (MRIs), sectioned images of the human body in real color and with high resolution have certain advantages in learning and teaching sectional anatomy (Spitzer & Whitlock, 1997; Dixon et al, 2015). Comparisons between the clinical images and the sectioned images are useful in many ways (Schiemann et al., 2000; Spitzer & Scherzinger, 2006; Nowinski et al, 2012).

Cats as pets are increasing in number, so that the use of CTs and MRIs to diagnose their diseases is becoming increasingly popular (Bishop et al., 2008; Taeymans et al., 2008; Kang et al., 2009; Weidner et al., 2012). The production of sectioned images of a cat that are helpful in learning CTs and MRIs of cats is becoming increasingly necessary (Rivero et al., 2005; Lauridsen et al., 2011). Moreover, three-dimensional (3D) models made from the sectioned images of a cat would be the source of a virtual dissection and virtual operation for veterinary students and veterinary physicians. Therefore, the sectioned images of a cat have been made by other researchers. However, the sectioning intervals of the images were too thick and inconstant; their resolution was too low (http://digimorph.org). The region of interest in the present study was the cat head because the morphology and function of the head of a species is quite different from those of other species.

The objective of this research was to help the interested people learn the sectional anatomy of cat head by presenting high-quality sectioned images. The original frontal planes of a cat head were stacked to obtain the other two orthogonal planes; on the high quality planes, meaningful head structures of cat were identified and compared with those of human (Park et al., 2009).

MATERIAL AND METHOD

A domestic short-haired female cat (one year old; height 460 mm; width 160 mm; length 711 mm) was purchased from a company supplying laboratory animals. This study was performed after obtaining permission from the Institutional Animal Care and Use Committee of Ajou University School of Medicine (No. AMC104).

The subject was sacrificed by an injection of potassium chloride (KCl; 100 mg/ml). Neither fixative nor dye was injected into the subject. After freezing in a -70 [degrees]C freezer, the whole body of a cat was positioned in the embedding box with a gelatin solution to be sectioned frontally. Using a cryomacrotome, the cat was ground serially, cranial to caudal, at 0.2 mm intervals to produce sectioned surfaces, as done previously with human cadaver (Park et al, 2005).

Each sectioned surface was photographed using a Canon[TM] EOS-1Ds Mark III digital camera (resolution 5,616 X 3,328; color depth 48 bit color) equipped with a Canon[TM] EF 50 mm f/1.2L USM lens. At that time, the sectioned surface was flashed by two Elinchrom[TM] Digital S strobes, connected to an Elinchrom[TM] Digital 2 power pack. The captured image (pixel size 0.1 mm) was saved in tagged image file format (TIFF) (Park et al., 2014). By repeating the procedure, 2,901 frontal sectioned images of the whole cat body were made. Among them, 466 images of the head were chosen; the excessive margins were cropped for this research (Fig. 1) (Table I).

The pixel size of the frontal planes was increased from 0.1 mm to 0.2 mm to standardize the pixel size to the sectioning interval; their bit depth was lowered from 48 bits color to 24 bit color. Using self-developed software, the frontal planes were stacked to produce dorsal and sagittal planes (Fig. 2) (Table I) (Park et al., 2010).

The three orthogonal planes of a cat head were observed minutely in conformity with the subregions. Furthermore, the sectioned images were compared with those of a human head (Park et al., 2009). If necessary, the length of a structure was measured according to the number of pixels on Photoshop CS6 (Adobe Systems, Inc., San Jose, CA, USA).

RESULTS

In the case of the original sectioned images of the cat, the file size of a single image was 32 MBytes and the total file size of the images of the whole body was 91.7 GBytes. In this study, images of the head region that were reduced in quantity were used. Consequently, the file sizes of single and total images were 3 Mbytes and 1.4 GBytes, respectively.

Cranium. Each bone of the cat cranium was identifiable by sutures. For example, on the lateral side of the cranium, the parietal and temporal bones were demarcated by a squamous suture. Unlike human, cat had a supernumerary bone, premaxilla in the front of the maxilla (Fig. 1A, E). In dog, the bone is called the incisive bone, and is usually long because of the protruded snout (Evans & Alexander, 2012).

Brain. Under the corpus callosum, other white matter (fornix, caudate nucleus, and internal capsule) could be observed. As an artifact, the lateral and third ventricles were collapsed with the cerebrospinal fluid discharged (Fig. 1C, G).

Ear. Cat had a larger tympanic cavity than human. In its tympanic cavity, the malleus, incus, and stapes were connected; a synovial joint between the malleus and incus was observed. The ratio of the largest length of the tympanic membrane and the oval window in the cat was approximately seventeen (Sebastiani & Fishbeck, 2005), while that in human was approximately ten (Jang et al., 2011; Park et al., 2013). This suggests that cats can collect sound well. The vestibular nerve and cochlear nerve were observed in the cochlea (Fig. 1D, H).

Eye. In the orbit of the cat, four rectus muscles and two oblique muscles could be observed just like human. Cat had one more muscle, retractor bulbi muscle, which retracts the eyeball. When the retractor bulbi muscle contracts, the nictitating membrane (third eyelid) is closed as a protective mechanism (Fig. 1B, F; Fig. 2). The nictitating membrane exists in many mammals, including dog (Sebastiani & Fishbeck; Evans & Alexander), but is vestigial in human (Grant et al., 1979).

Cat had a large pupil in the eyeball to make a broad visual field. Compared to human, the large pupil makes it possible to perceive light of only one-sixth the intensity (Wilcox & Barlow, 1975). The cat's cornea was more curved, so that the anterior chamber was greater, which gives rise to higher refraction of light (Fig. 2) (Sebastiani & Fishbeck). In the center of the retractor bulbi muscles, the optic nerve was identified. The optic nerve passing optic canal ran from the eyeball to the optic chiasm; the optic tract ran from the optic chiasm to the lateral geniculate nucleus; the optic radiation ran from the lateral geniculate nucleus to the calcarine sulcus (Fig. 3).

Nose. Cat had well-developed olfactory structures that were comprised of a considerably larger olfactory bulb in the cerebrum than human (Fig. 1) (Sebastiani & Fishbeck). The olfactory sense of cat is approximately 14 times better than of that of human. In a 70kg human, the surface area of the olfactory epithelium is approximately 10 [cm.sup.2]. In contrast, a 3kg cat has approximately 20 [cm.sup.2] of olfactory epithelium (Purves et al., 2004).

Cat had a vomeronasal organ duct connecting the rear of the incisors to the lateral side of the nasal septum (Fig. 4). The vomeronasal organ is known to detect pheromones and control sexual behavior (D0ving & Trotier, 1998).

Mouth. A small salivary gland, the infraorbital gland, which was not found in human, was located in the orbit beneath the eye (Fig. 1B). Cat had the masseter, temporal, lateral pterygoid, and medial pterygoid muscles, as does human. The masticatory muscles were relatively larger than in human (Fig. 1). The maxillary and mandibular nerves were found within the masticatory muscles (Fig. 1C). The inferior alveolar nerve of the mandibular nerve passed through the mandibular canal (Fig. 1B). The ratio of the largest cross length of the trigeminal nerve to that of the brainstem in cat was approximately one-seventh (1,070 pixels out of 7,380 pixels), while the ratio in human was approximately one-twelfth (2,101 pixels out of 26,396 pixels). This means that the trigeminal nerve of a cat was relatively thick (Fig. 5) (Sebastiani & Fishbeck). Contrary to human, the cat's maxillary nerve was much thicker than the mandibular nerve (Fig. 1B,C), because the long whiskers around cat nose are well innervated by the maxillary nerve (Gordon et al, 1961).

DISCUSSION

This is the first report to describe the detailed head structures of a cat on sectioned images with real color and high resolution. Moreover, the morphological characteristics have been explained with the proper physiology of a cat. These minute findings are possible thanks to the state-ofthe-art sectioned images as follows.

First, no fixative or dye is injected; images contain 48 or 24 bit color (Table I), so that original body color was visible. Second, 0.1 or 0.2 mm-sized pixels allow the identification of extremely small structures (e.g., auditory ossicles) (Fig. 1). Third, consistent intervals (0.2 mm) of the sectioned images enable a structure to be traced from the origin to destination (e.g., visual pathway) (Fig. 3).

Comparative anatomy of the head between cat and human is valuable. This is because the morphology of the human head has been studied widely, whereas there have been fewer studies of a cat's head (Fig. 5).

In this research, the cat head and human head were compared (Fig. 5). The comparison was reliable because the sectioned images of a cat and those of human were manufactured under similar conditions (Park et al., 2009).

On the other hand, the directions of the sectioned images were different in the two species. To overcome the problem, 3D models of the structures of a cat should be compared with those of human. For a future study, the structures will be delineated to acquire segmented images; the segmented images of each structure would be stacked and a 3D reconstruction will be carried out. The resulting models of a cat will be compared with those of a human to demonstrate the fine topographic anatomy regardless of the sectioning directions (Park et al, 2013).

In detail, the segmented images would produce sophisticated 3D surface and volume models of cat (Park et al., 2013, 2014). The volume model of cat whole body based on these sectioned images is being used in the Anatomage Table[TM] (Copyright 2005[C] Anatomage, USA; http:// www.anatomage.com), which offers a life-size interactive anatomy visualization (Fig. 6).

For other interested researchers, the sectioned images will be distributed free of charge. An additional goal of this report is to inform other researchers of the methods to establish those raw images. It is expected that other researchers will develop even better sectioned images of cat. The sectioned images and 3D models produced during this research are expected to be viewed as educational and training tools for clinical veterinary medicine.

ACKNOWLEDGEMENTS

This research was financially supported by the Ministry of Trade, Industry and Energy (MOTIE) and Korea Institute for Advancement of Technology (KIAT) through the International Cooperative R&D program (Grant number: N0002249).

The authors gratefully acknowledge the support provided by Korea Institute of Science & Technology Information (KISTI).

REFERENCES

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Park, H. S.; Chung, M. S.; Shin, D. S.; Jung, Y. W. & Park, J. S. Accessible and informative sectioned images, color-coded images, and surface models of the ear. Anat. Rec., 296(8):1180-6, 2013.

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Corresponding author:

Jin Seo Park

Department of Anatomy

Dongguk University School of Medicine

87 Dongdae-ro,

Gyeongju, 38067

REPUBLIC OF KOREA

E-mail: park93@dongguk.ac.kr

Received: 26-12-2017

Accepted: 29-01-2018

Beom Sun Chung (1); Min Suk Chung (1); Seung-Bock Lee (2); Cheong Youn (3) & Jin Seo Park (4)

(1) Department of Anatomy, Ajou University School of Medicine, 164 Worldcup-ro, Suwon, 16499, Republic of Korea.

(2) Scientific Data Strategy Lab., Scientific Data Research Center, Korea Institute of Science and Technology Information, 245 Daehak-ro, Daejeon, 34141, Republic of Korea.

(3) College of Engineering, Chungnam National University, 99 Daehak-ro, Daejeon, 34134, Republic of Korea.

(4) Department of Anatomy, Dongguk University, School of Medicine, 87 Dongdae-ro, Gyeongju, 38067, Republic of Korea.

Caption: Fig. 1. Frontal sectioned images of a cat head. In (A-D) original and (E-H) magnified sectioned images on the level of nose (A, E), eye (B,F), cerebrum (C, G), and ear (D, H), detailed structures of cat are identified. b. = bone; m. = muscle; n. = nerve; a. = artery; inf.= inferior; lat. = lateral; med. = medial; int. = internal.

Caption: Fig. 2. Sectioned images of three orthogonal planes passing the eyeball. In (A) frontal, (B) dorsal, and (C) sagittal sectioned images, eye structures including the eyelid and anterior chamber are shown.

Caption: Fig. 3. Visual pathway on frontal sectioned images. Visual pathway from the eyeball to the lateral geniculate nucleus.

Caption: Fig. 4. Vomeronasal duct on the frontal sectioned images. On the images from the oral to nasal cavities, the vomeronasal duct (green arrow) can be found.

Caption: Fig. 5. Size of the brainstem and trigeminal nerve of cat and human. Ratio of the size of the trigeminal nerve (green arrows) to that of the brainstem (yellow arrows) in (a) cat and (b) human are about 1/7 (1,070 pixels / 7,380 pixels) and 1/12 (2,101 pixels / 26,396 pixels), respectively.

Caption: Fig. 6. Volume model of cat whole body ofthe Anatomage Table[TM] made from the sectioned images provided by the authors (Chung et al, 2015).
Table I. Features of the three orthogonal planes of a cat head.

Planes    Intervals  Numbers  Resolution   Pixel size  Bit depth

Frontal   0.2 mm     466      1,154 X 928  0.1 mm      48 bit color
Dorsal    0.2 mm     464      577 X 466    0.2 mm      24 bit color
Sagittal  0.2 mm     577      464 X 466    0.2 mm      24 bit color
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Author:Chung, Beom Sun; Chung, Min Suk; Lee, Seung-Bock; Youn, Cheong; Park, Jin Seo
Publication:International Journal of Morphology
Date:Jun 1, 2018
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