Saxitoxin puffer fish poisoning in the United States, with the first report of Pyrodinium bahamense as the putative toxin source.
METHODS: We used the standard mouse bioassay (MBA), receptor binding assay (RBA), mouse neuroblastoma cytotoxicity assay (MNCA), Ridascreen ELISA, MIST Alert assay, HPLC, and liquid chromatography-mass spectrometry (LC-MS) to determine the presence of STX, decarbamoyl STX (dc-STX), and N-sulfocarbamoyl (B1) toxin in puffer fish tissues, clonal cultures, and natural bloom samples of Pyrodinium bahamense from the IRL.
RESULTS: We found STXs in 516 IRL southern (Sphoeroides nephelus), checkered (Sphoeroides testudineus), and bandtail (Sphoeroides spengleri) puffer fish. During 36 months of monitoring, we detected STXs in skin, muscle, and viscera, with concentrations up to 22,104 [micro]g STX equivalents (eq)/100 g tissue (action level, 80 [micro]g STX eq/100 g tissue) in ovaries. Puffer fish tissues, clonal cultures, and natural bloom samples of P. bahamense from the IRL tested toxic in the MBA, RBA, MNCA, Ridascreen ELISA, and MIST Alert assay and positive for STX, dc-STX, and B1 toxin by HPLC and LC-MS. Skin mucus of IRL southern puffer fish captive for 1-year was highly toxic compared to Florida Gulf coast puffer fish. Therefore, we confirm puffer fish to be a hazardous reservoir of STXs in Florida's marine waters and implicate the dinoflagellate P. bahamense as the putative toxin source.
CONCLUSIONS: Associated with fatal paralytic shellfish poisoning (PSP) in the Pacific but not known to be toxic in the western Atlantic, P. bahamense is an emerging public health threat. We propose characterizing this food poisoning syndrome as saxitoxin puffer fish poisoning (SPFP) to distinguish it from PFP, which is traditionally associated with tetrodotoxin, and from PSP caused by STXs in shellfish.
KEY WORDS: dinoflagellate, Florida, harmful algae, puffer fish, Pyrodinium bahamense, saxitoxin puffer fish poisoning, saxitoxins, Sphoeroides spp. Environ Health Perspect 114:1502-1507 (2006). doi:10.1289/ehp.8998 available via http://dx.doi.org/ [Online 6 July 2006]
Puffer fish poisoning (PFP) is usually caused by ingestion of tetrodotoxins (TTXs) found naturally in certain species of puffer fish (Halstead 1967; Mosher and Fuhrmann 1984). In Japan, 20-100 people die annually from PFP, in spite of stringent controls by authorities (Ogura 1971). TTXs can cause fatal human poisoning, which is similar to paralytic shellfish poisoning (PSP) caused by saxitoxins (STXs). PSP is caused by the consumption of toxic shellfish (Shumway 1990) and rarely by fish that have have become toxic after feeding on STX-producing microalgae (Maclean 1979). As well as TTXs, STXs have also been found in at least 12 marine and freshwater puffer fish species in Asia (Ahmed et al. 2001; Kodama et al. 1983; Kungsuwan et al. 1997; Nakamura et al. 1984; Nakashima et al. 2004; Sato et al. 1997, 2000; Zaman et al. 1997), but their bioorigin has not been identified.
TTXs are chemically distinct from STXs, but both neurotoxins produce similar symptoms in mammals because they act on site 1 of the voltage-dependent sodium channel, blocking the influx of sodium into excitable cells and restricting signal transmission along nerve and muscle membranes (Ahmed 1991). The symptoms of traditional PFP from TTXs and of PSP from STXs include tingling and numbness of the mouth, lips, tongue, face, and fingers; paralysis of the extremities; nausea; vomiting; ataxia; drowsiness; difficulty in speaking; progressively decreasing ventilatory efficiency; and finally in extreme cases, death by asphyxiation caused by respiratory paralysis (Ahmed 1991; Catterall 1985; Kao 1993).
PFP cases in Europe (Kao 1993) and Mexico (Nunez-Vazquez et al. 2000) have occasionally been reported. In the United States, PFP has been associated with imports of puffer fish [Centers for Disease Control and Prevention (CDC) 1996]; rarely have fatalities occurred after the consumption of indigenous puffer fish. In Hawaii, white-spotted puffer fish, Arothron hispidus, were implicated in seven deaths (Ahmed 1991). Until 1974, seven PFP cases in Florida, outside of the Indian River Lagoon (IRL), were caused by the consumption of locally caught "blowfish" or puffer fish (Ahmed 1991; Benson 1956; Bigler 1999; Hemmert 1974; Mosher and Fuhrmann 1984). These cases included three fatalities, likely from TTX; for example, one woman died 45 min after consuming toxic liver from a checkered puffer fish (Sphoeroides testudineus) (Benson 1956). The toxins involved in the previous Florida PFP cases were not characterized, but because PFP is usually associated with TTX, investigators likely assumed that TTX was the cause (Benson 1956; Bigler 1999; Hemmert 1974). Tissues from Florida bandtail (Sphoeroides spengleri), checkered, and southern puffer fish (Sphoeroides nephelus) were found to be lethal in the mouse bioassay (MBA) (Burklew and Morton 1971; Lalone et al. 1963), but, again, the toxins were not determined.
Until January 2002 the harvest and consumption of puffer fish from the IRL was not a risk to public health. Since then (until May 2004), however, 28 PFP cases occurring in Florida (n = 21), New Jersey (n = 3), Virginia (n = 2), and New York (n = 2) caused by puffer fish originating from the IRL were reported (Bodager 2002; CDC 2002a, 2002b). Analyses of toxins from unidentified puffer fish fillet remnants from one of the early 2002 PFP cases in New Jersey revealed STXs (Quilliam et al. 2004), not TTXs, a distinction that alone could not be made on the basis of consumer symptoms or traditional screening methods (i.e., MBA).
During 2002-2004, all PFP cases were linked to puffer fish originating from the northern IRL and the Banana River on Florida's east coast (Figure 1). Except for one case, where puffer fish were commercially harvested and reached a New Jersey fish market, puffer fish were caught recreationally [Bodager 2002; Florida Fish and Wildlife Conservation Commission (FWC) 2004]. In April 2002, state and federal officials issued health advisories, and the FWC banned puffer fish harvesting in the IRL, a ban that currently remains in effect. In New York on 14 October 2002, two PFP cases were caused from fish caught near Titusville, Florida, but frozen in March 2002 before the harvesting ban (Bodager D, personal communication). This case demonstrated the stability of toxins in puffer fish frozen for almost 9 months.
Because STXs had not previously been identified in Florida's marine waters and their distribution, source, and origin were unknown in April 2002 (Abbott et al. 2003; Landsberg et al. 2002), we initiated an intensive survey of biota in the IRL. In this article we present a summary from 3 years of monitoring, as well as the first report of the putative toxin source.
Materials and Methods
Field collections. From April 2002 through April 2005, southern, checkered, and bandtail puffer fish (n = 516) were harvested via a range of fishing gear from the original source locations of the PFP incidents in the northern and central IRL (Figure 1). The fish were shipped biweekly or monthly on ice to the FWC's Fish and Wildlife Research Institute (FWRI) or to the Food and Drug Administration's (FDA) Center for Food Safety and Applied Nutrition Washington Seafood Laboratory and frozen in individual sealable plastic bags until required for toxicity testing.
Live phytoplankton samples were collected routinely with a 62-[micro]m mesh plankton net at multiple locations along the IRL; also, a 1-L water bottle was used to directly sample a phytoplankton bloom. Water samples were transported to FWRI at ambient temperature.
Live puffer fish. To determine if puffer fish maintained toxicity once they were removed from the putative toxin source, we kept puffer fish in captivity. We obtained southern puffer fish by rod and line or by seine net from the IRL near Titusville (Atlantic coast) (n = 2) and from Tampa Bay (Gulf coast), Florida (n = 2), and transported them live in ambient seawater to the wet laboratory at FWRI. Southern puffer fish were individually held in covered, 80-L aquaria in 25 psu (practical salinity units) artificial sea water (Instant Ocean; Aquarium Systems, Inc., Mentor, OH) and fed shrimp or squid that originated from nontoxic locations. We measured water quality daily and routinely carried out 30% water exchanges. After several weeks acclimation, we tested fish skin mucus bimonthly by lightly anesthetizing the fish [100 ppm tricaine methanesulfonate (MS-222; Sandoz Pharmaceuticals Corp., Basel, Switzerland) in 4 L], placing the fish on a dissection board, and collecting the mucus on a preweighed 47-mm-diameter, glass-fiber filter (Whatman, Clifton, NJ) by gently rubbing the paper along both sides of the body.
Fish care. We conducted research in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals. All fish were treated humanely and with regard for alleviation of suffering, according to the Guide for Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources 1996).
Preparation of tissues. Within several weeks of collection, we thawed frozen puffer fish, measured standard lengths and wet weights, and removed skin, liver, stomach, intestinal tract, muscle, and gonads.
Pyrodinium bahamense cultures. We established 11 clonal nonaxenic cultures of P. bahamense from IRL samples, using the micropipette technique to isolate single cells. We maintained batch cultures in environmental chambers at near-ambient light and temperature conditions (35 microEinsteins/[m.sup.2]/sec, 25[degrees]C) and at salinities of 20-36 psu. Growth media consisted of filtered, autoclaved natural offshore seawater enriched to ES-DK (enriched natural seawater medium modified by D. Kulis) (Kulis D, personal communication; Kokinos and Anderson 1995) levels with the addition of [10.sup.-7] M selenium (as sodium selenite).
Toxin detection. At various stages of this survey, we tested puffer fish tissues for STX bioactivity using the standard MBA, Ridascreen ELISA (R-Biopharm GmBH, Darmstadt, Germany), MIST Alert (Jellet Biotek, Dartmouth, Canada) PSP kit, and mouse neuroblastoma cytotoxicity assay (MNCA, Neuro-2A) and receptor-binding assay (RBA) [Association of Official Analytical Chemists (AOAC) 1990; Cembella et al. 2003; Jellett et al. 2002; Luckas et al. 2003; Powell and Doucette 1999; Ruberu et al. 2003; Usleber et al. 1991]. We also prepared selected samples for toxin characterization and confirmation by HPLC (Thermo Electron Corporation, San Jose, CA), with postcolumn oxidation and fluorescence detection and liquid chromatography-mass spectrometry (LC-MS) (Waters Corporation, Milford, MA) (Negri et al. 2003; Oshima 1995) using in-house FDA reference standards. We split tissue samples for interlaboratory calibrations and then extracted them by one of two methods. For bioactivity assays, tissue samples were homogenized and weighed (wet weight) into glass test tubes. Samples were extracted using 0.1 N HCl, adjusted to pH 2.5-4, boiled for 5 min in a boiling water bath, centrifuged at 3,000 x g for 10 min, and the supernatant retained for toxin testing. For toxin characterization by HPLC and LC-MS, tissue splits were extracted with 0.1 M aqueous acetic acid, centrifuged, and the supernatants filtered (0.22 [micro]m).
After the initial 2002 saxitoxin puffer fish poisoning (SPFP) events, 11 southern puffer fish were divided into the tissue compartments (listed above), and tissue samples were extracted by boiling in 0.1 N HCl (Washington Seafood Laboratory) and analyzed for toxic activity using three independent methods. MBAs were performed at the Washington Seafood Laboratory; MNCAs were performed at the FDA Gulf Coast Seafood Laboratory; and RBAs were performed at the National Oceanic and Atmospheric Administration National Ocean Service Center for Coastal Environmental Health and Biomolecular Research.
Clonal cultures and natural bloom samples of P. bahamense were filtered onto 25-mm glass-fiber filters (Whatman) or centrifuged at 3,000 x g for 5 min and then extracted. Puffer fish mucus or Pyrodinium samples on filters were homogenized in 0.1 N HCl using a ground-glass tissue grinder and treated as above. Pyrodinium extracts were tested for toxicity by ELISA and MBA and characterized for toxin profile using HPLC and LC-MS. Puffer fish mucus was tested for toxicity by ELISA.
Electron microscopy. We prepared natural field samples or clonal cultures of P. bahamense for the scanning electron microscope (SEM) using standard fixation methods (Truby 1997). Pyrodinium samples were added to unacidified Lugol's at a dilution of 1:100 in suspension, collected onto a 5-[micro]m polycarbonate filter, secondarily fixed with 4% paraformaldehyde for 20 min, washed with water, dehydrated in an ethanol series followed by a freon series, critical-point-dried using carbon dioxide, mounted onto aluminum stubs using carbon adhesive tape, sputter-coated with gold/palladium, and photographed with a Cambridge Stereoscan 240 SEM (Cambridge Instruments, Cambridge, UK).
Puffer fish toxin analyses. During 36 months of continuous monitoring after the initial SPFP events, STXs were routinely detected in the skin, muscle, viscera, and gonads of 516 puffer fish (southern, n = 402; checkered, n = 105; bandtail, n = 9), which tested toxic in MBA, RBA, Ridascreen ELISA, and MIST Alert assays. By ELISA, maximum STX levels in the muscle fillet were well above the action level [80 [micro]g STX equivalents (eq)/100 g tissue] in southern puffer fish (maximum, 14,571 [micro]g STX eq/100 g tissue, mean = 938.4) and just over the action limit in bandtail (maximum, 364.5 [micro]g STX eq/100 g tissue; mean, 121.7) and checkered puffer fish (maximum, 104.3 [micro]g STX eq/100 g tissue; mean, 6.9) (Table 1). Maximum STX concentrations in the liver of southern and checkered puffer fish were 1,443 and 51.1 [micro]g STX eq/100 g tissue, respectively. The highest tissue concentration, 22,104 [micro]g STX eq/100 g tissue, was measured in the ovaries of a southern puffer fish (data not shown).
All three assays (MBA, MNCA, and RBA) confirmed elevated concentrations of toxic activity in the muscle compared to the liver (5- to 20-fold) of 11 southern puffer fish (Table 2). By MBA, MNCA, and RBA, ranges of STX concentrations in muscle were 197-5,264 (mean [+ or -] SD, 2,302.3 [+ or -] 1,539.3), 120-2,294 (957.7 [+ or -] 659.5), and 198-6,091 (2,439 [+ or -] 1,995.3) [micro]g STX eq/100 g tissue, respectively. By MBA, MNCA, and RBA, STX concentrations in liver were 83-1,034 (mean [+ or -] SD, 282.6 [+ or -] 261.5), 60-420 (169.1 [+ or -] 106.2) and 16-711 (223.1 [+ or -] 186.5) [micro]g STX eq/100 g tissue, respectively (Table 2).
Skin mucus of IRL southern puffer fish held captive for 1 year was highly toxic (2,407-9,039 [micro]g STX eq/100 g) compared with that of Florida Gulf Coast southern puffer fish (6.25-140 [micro]g STX eq/100 g). Over a period of at least 6 months, STX levels in the IRL southern puffer fish fluctuated but remained at highly toxic concentrations.
Toxin profiles in unconsumed puffer fish fillets (n = 4) from a 2004 PFP event were confirmed by HPLC (Figure 2A,B) and LC-MS (Figure 2C-F) to be STX (92.4% [+ or -] 3.1), decarbamoyl saxitoxin (dcSTX; 6.9% [+ or -] 2.4), and N-sulfocarbamoyl B1 toxin (B1; 0.7% [+ or -] 0.7) as originally found in a 2002 PFP case (Quilliam et al. 2004).
We also detected TTX (quantified by MBA and confirmed by LC-MS) in IRL checkered puffer fish (n = 3) at concentrations of 1,553 [+ or -] 919 and 53,700 [+ or -] 19,212 [micro]g TTX/100 g in the muscle and liver, respectively (Figure 3A-D).
Pyrodinium bahamense toxin analyses. All clonal cultures (n = 11) and natural bloom samples (n = 2) (> 3 million cells/L) of P. bahamense (Figure 4) obtained from the IRL tested positive for STX by HPLC, LC-MS, Ridascreen ELISA, MIST Alert, and RBA assays. Toxin concentrations for P. bahamense isolates (n = 11) ranged from 1.68 to 25.57 pg STX eq/cell (as determined by ELISA). Further analysis of five of these isolates using HPLC determined that the toxin profile was composed of B1 (91.1% [+ or -] 2.2, mean [+ or -] SD) and STX (8.9% [+ or -] 2.2), with integrated toxicity values ranging from 2.02 to 12.74 pg STX eq/cell. The HPLC toxin profile of a 2002 bloom sample at 3.28 pg STX eq/cell was composed of STX (26%), B1 (73%), and dcSTX (1%) (Figure 5A,B).
PFP cases have been associated with STXs in Asia (Ahmed et al. 2001), but the IRL incidents are the first in which STX poisoning has been confirmed in puffer fish originating in the United States (Quilliam et al. 2004). The high and low concentrations of STXs and TTX, respectively, in the muscle of IRL puffer fish are similar to those found in Philippine (Sato et al. 2000) and Japanese (Kodama et al. 1984) puffer fish, although in the latter, visceral toxicity from TTX is high and fish-poisoning incidents usually occur after consumption of fillet(s) contaminated due to improper preparation. Unlike the tissue distribution of TTXs reported previously in various puffer fish species (Kodama et al. 1984), STXs in IRL southern puffer fish have been consistently much higher in the muscle than in the liver and, in many individual fish, were more than two orders of magnitude above the action limit. Therefore, even careful preparation of IRL puffer fish fillets would not prevent intoxication in consumers. Interestingly, the confirmation of extremely high concentrations of TTX in the liver of checkered puffer fish suggests that the earlier-reported fatality from the consumption of this species in south Florida (Benson 1956) was likely caused by this toxin and not STX.
The MBA, the traditional screening method for PFP, does not distinguish between STXs and TTXs. New reports in Asia (Ahmed et al. 2001; Nakashima et al. 2004; Sato et al. 2000) have found both toxin groups co-occurring in puffer fish species previously thought to contain only TTX. Both our results and these reports suggest that STXs in puffer fish may be more widespread than previously thought; therefore, comprehensive analytical assessments of PFP incidents are needed to distinguish TTX from STX. We propose that the food-poisoning syndrome caused by intoxication from STX exposure from fish should be characterized as SPFP to distinguish it from PFP, which is caused by--but not always verified to be from--TTX, and to distinguish SPFP from PSP associated with STXs in shellfish.
In a 1960s toxicity study of IRL southern puffer fish [erroneously identified by Lalone et al. (1963) as northern puffer fish, Sphoeroides maculatus, which are not found in the IRL and occur only as far south as Jacksonville, FL (Shipp and Yerger 1969a, 1969b; Tremain and Adams, 1995)], muscle was demonstrated to be toxic to mice by intraperitoneal injection. However, the toxins in these puffer fish samples were not characterized. Of the tissues investigated in that study, including skin, liver, muscle, and testes or ovary, the muscle was the most lethal to mice, similar to the pattern seen today. Although this anecdotal evidence suggests that southern puffer fish may have been mildly toxic from STX in the IRL for the past 45 years, there has been no indication that toxin levels were even close to the order of magnitude observed since 2002 nor was the FDOH informed of any poisoning incidents from this area prior to this time.
Globally, human food-poisoning incidents from STX exposure are usually caused by toxic marine shellfish (Kao 1993) that filter-feed on STX-producing microalgae. PSP can be fatal (Kao 1993), but the successful implementation of programs monitoring STX-producing microalgae and STXs in shellfish has helped minimize the risk of toxin exposure to humans. In marine waters, PSP is caused by toxic dinoflagellates, where STXs are produced by more temperate Alexandrium species and Gymnodinium catenatum and by tropical Pyrodinium bahamense var. compressum (Kao 1993). PSP in the United States has been limited to New England and the Pacific West Coast, including Alaska, and has only been associated with STXs produced by temperate Alexandrium spp. in these areas (Gessner 2000).
The epidemiology of PSP incidents is related to the global distribution of the various STX-producing species and their toxigenic strains. PSP outbreaks due to P. bahamense have caused more fatalities than any other microalgal species known (Usup and Azanza 1998). In 1987, PSP associated with P. bahamense var. compressum in Champerico, Guatemala, hospitalized at least 187 individuals and resulted in 26 fatalities (Rodrigue et al. 1990). Before 1996, 1,768 cases of PSP with 107 deaths had been reported in the Philippines, mostly attributable to P. bahamense var. compressum (Babaran et al. 1998). These fatalities were largely due to the sudden appearance of P. bahamense in areas previously unknown to contain toxic species, because monitoring activities were not in place or because hospital facilities had not treated people in these previously unaffected areas (Kao 1993).
In the present study we confirm unequivocally that puffer fish are a primary reservoir of STXs in marine waters in Florida, and we implicate for the first time the tropical western Atlantic dinoflagellate P. bahamense as the source of toxicity. We found the STX profile of P. bahamense isolates from Florida to be similar to, but proportionately different from, the toxin profile of southern puffer fish fillet (Etheridge et al. 2006; Quilliam et al. 2004), and we identified P. bahamense as the putative source of the STXs. Confirmatory toxin-transfer studies from Pyrodinium via shellfish to puffer fish are in progress. Although many temperate marine Alexandrium species, Gymnodinium catenatum, and a few freshwater cyanobacteria species produce STXs (Kodama 2000), these organisms have not been found in the IRL.
In addition to the Caribbean and Gulf coasts of Mexico, bioluminescent P. bahamense blooms are found only along Florida's Atlantic and Gulf coasts (Badylak et al. 2004; Phlips et al. 2004; Steidinger et al. 1980). However, until the IRL SPFP incidents, the Atlantic/Caribbean P. bahamense var. bahamense was not known to be toxic (Steidinger et al. 1980), unlike the Pacific P. bahamense var. compressum found in Asia and the Pacific Coast of Central America (Rodrigue et al. 1990; Usup and Azanza 1998; Vargas-Montero and Freer 2004). The Atlantic P. bahamense var. bahamense was separated from the Pacific P. bahamense var. compressum based on morphologic criteria and evident lack of toxicity in the former variety (Steidinger et al. 1980). Based on our initial findings, we are testing the hypothesis that this varietal distinction may no longer be valid and that P. bahamense is all one species.
Florida has many toxigenic marine algal species, but none were known to produce STXs (Steidinger et al. 1999). It is conceivable that STXs might have appeared in the IRL because of one of several scenarios: a) toxigenic populations of Pyrodinium have been introduced; b) ecologic conditions have changed and have induced toxicity in a variety that was previously nontoxic; c) toxic Pyrodinium was present but produced toxins at undetectable concentrations; or d) ecologic conditions have changed and increased the food-web exposure of susceptible biota to toxins. We believe that c is the most likely scenario. In the IRL there is a history of Pyrodinium (Badylak et al. 2004; Phlips et al. 2004), and as mentioned previously, there is a historical precedent for low-level toxicity in puffer fish.
In the past few years, the northern IRL has experienced a number of unusual events: dolphin, manatee, fish, and horseshoe crab mortalities; increased tumor incidence in hard clams; diseased shrimp; and reductions in the natural recruitment of and increases in the hatchery losses of hard clams (Bossart et al. 2003; Landsberg et al. 2002; Landsberg and Kiryu 2005). To what extent, if at all, these events are linked to the emerging issue of toxic P. bahamense blooms remains undetermined. The significant risk of SPFP and PSP from saxitoxins in the IRL has been assessed and management plans implemented accordingly. Thus far, routine monitoring by Florida state agencies has determined that STX levels in shellfish, principally hard clams (Mercenaria spp.), are not a significant risk to public health (Landsberg et al. 2005). The extreme toxicity of puffer fish fillet, well above the action level, emphasizes the danger that puffer fish pose to the public and supports the permanent ban on their harvest in this area (FWC 2004).
The widespread implications for public health incidents from the tropical western Atlantic P. bahamense remain unknown. Public health officials and natural resource managers should be aware of these new findings and remain vigilant to examine any potential association between the co-occurrence of this species throughout its range and the appearance of toxic food-poisoning incidents.
Abbott J, Landsberg JH, Flewelling LJ, Baird L, Fisk S. 2003. Transfer of saxitoxins within the Indian River Lagoon, Florida food web [Abstract]. In: Second Symposium on Harmful Marine Algae in the U.S., 9-13 December 2003, Woods Hole, MA. Woods Hole, MA:Woods Hole Oceanographic Institution, 1-2. Available: http://www.whoi.edu/redtide/2ndsymposium/SE-GoMx_abstracts.pdf [accessed 20 October 2005].
Ahmed FE, ed. 1991. Seafood Safety. Washington DC:National Academy Press.
Ahmed MS, Jaime E, Reichelt M, Luckas B. 2001. Paralytic shellfish poisoning in freshwater puffer fish (Tetraodon cutcutia) from the River Burigonga, Bangladesh. In: Harmful Algal Blooms 2000 (Hallegraeff GM, Blackburn SI, Bolch CJ, Lewis RJ, eds). Paris:United Nations Educational, Scientific and Cultural Organization, 19-21.
AOAC. 1990. Paralytic shellfish poison. Biological method. Final action. In: Official Methods of Analysis (Hellrich K, ed). Arlington, VA:Association of Official Analytical Chemists, 881-882.
Babaran R, Espinosa R, Abalos T. 1998. Initiating and triggering mechanisms causing harmful algal blooms. J Shellfish Res 17:1623-1626.
Badylak S, Kelley K, Phlips EJ. 2004. A description of Pyrodinium bahamense (Dinophyceae) from the Indian River Lagoon, Florida, USA. Phycologia 43:653-657.
Benson J. 1956. Tetradon (blowfish) poisoning. A report of two fatalities. J Forensic Sci 1:119-126.
Bigler W. 1999. Florida past--bizarre tales about bats, bongos and blowfish. EPI Update 24 December. Available: http://www.doh.state.fl.us/disease_ctrl/epi/Epi_Updates/1999/eu991224.html [accessed 16 October 2005].
Bodager D. 2002. Outbreak of saxitoxin illness following consumption of Florida pufferfish. Fl J Environ Health 179:9-13.
Bossart GD, Meisner R, Varela R, Mazzoil M, McCulloch S, Kilpatrick D, et al. 2003. Pathologic findings in stranded Atlantic bottlenose
dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida. Fla Sci 66:226-238.
Burklew MA, Morton RA. 1971. The toxicity of Florida Gulf puffers, genus Spheoroides. Toxicon 9:205-210.
Catterall WA. 1985. The voltage-sensitive sodium channel: a receptor for multiple toxins. In: Toxic Dinoflagellates (Anderson DM, White AW, Baden DG, eds). New York: Elsevier, 329-342.
Cembella AD, Doucette GJ, Garthwaite I. 2003. In vitro assays for phycotoxins. In: Manual on Harmful Marine Microalgae (Hallegraeff GM, Anderson DM, Cembella AD, eds). Paris:United Nations Educational, Scientific and Cultural Organization, 297-345.
CDC (Centers for Disease Control and Prevention). 1996. Tetrodotoxin poisoning associated with eating puffer fish transported from Japan--California, 1996. MMWR Morb Mortal Wkly Rep 45:389-391.
CDC (Centers for Disease Control and Prevention). 2002a. Neurologic illness associated with eating Florida pufferfish, 2002. MMWR Morb Mortal Wkly Rep 51:321-323.
CDC (Centers for Disease Control and Prevention). 2002b. Update: neurologic illness associated with eating Florida pufferfish, 2002. MMWR Morb Mortal Wkly Rep 51:414-416.
Etheridge S, Deeds J, Hall S, White K, Flewelling L, Abbott J, et al. 2006. Detection methods and their limitations: PSP toxins in Florida puffer fish responsible for human poisoning events in 2004. Afr J Mar Sci 28:383-387.
FWC (Florida Fish and Wildlife Conservation Commission). 2004. Puffer Fish Toxicity in Florida's Indian River Lagoon. Available: http://www.floridamarine.org/features/view_article.asp?id=18918 [accessed 20 October 2005].
Gessner BD. 2000. Neurotoxic Toxins. In: Seafood and Freshwater Toxins (Botana LM, ed). New York:Marcel Dekker, 65-90.
Halstead BW. 1967. Poisonous and Venomous Marine Animals of the World, Vol 1-3. Washington:U.S. Government Printing Office.
Hemmert CD. 1974. Tetraodon (pufferfish) poisoning. Memorandum of the Florida Department of Health, Tallahassee, Florida. In: Seafood Safety (Ahmed FE, ed). Washington DC:National Academy Press, 100.
Institute of Laboratory Animal Resources. 1996. Guide for the Care and Use of Laboratory Animals. Washington, DC:National Academy Press.
Jellett JF, Roberts RL, Laycock MV, Quilliam MA, Barrett RE. 2002. Detection of paralytic shellfish poisoning (PSP) toxins in shellfish tissue using MIST Alert, a new rapid test, in parallel with the regulatory AOAC mouse bioassay. Toxicon 40:1407-1425.
Kao CY. 1993. Paralytic shellfish poisoning. In: Algal Toxins in Seafood and Drinking Water (Falconer IR, ed). London: Academic Press, 75-86.
Kodama M. 2000. Ecobiology, classification, and origin. In: Seafood and Freshwater Toxins (Botana LM, ed). New York:Marcel Dekker, 125-149.
Kodama M, Ogata T, Kawamukai K, Oshima Y, Yasumoto T. 1984. Toxicity of muscle and other organs of five species of puffer collected from the Pacific coast of Tohoku area of Japan. Bull Jap Soc Sci Fish 50:703-706.
Kodama M, Ogata T, Noguchi T, Maruyama J, Hashimoto K. 1983. Occurrence of saxitoxin and other toxins in the liver of pufferfish Takifugu pardalis. Toxicon 21:897-900.
Kokinos JP, Anderson DM. 1995. Morphological development of resting cysts in cultures of the marine dinoflagellate Lingulodinium polyedrum (= L. machaerophorum). Palynology 19:143-166.
Kungsuwan A, Arakawa O, Promdet M, Onoue Y. 1997. Occurrence of paralytic shellfish poisons in Thai freshwater puffers. Toxicon 35:1341-1346.
Lalone RC, DeVillez D, Larson E. 1963. An assay of the toxicity of the Atlantic pufferfish, Spheroides maculatus. Toxicon 1:159-164.
Landsberg JH, Abbott JP, Flewelling LJ, Scott PS, Wolny J. 2005. Saxitoxin monitoring in Florida: one more toxin to deal with [Abstract]. In: Third Symposium on Harmful Algae in the U.S., 3-6 October 2005, Pacific Grove, CA. Monterey Bay, CA: Monterey Bay Aquarium Research Institute., 44. Available: http://www.whoi.edu/redtide/3rdsymposium/AbstractBook.pdf [accessed 27 March 2006].
Landsberg JH, Hall S, Johannessen J, White K, Conrad S, Flewelling LJ, et al. 2002. Puffer fish poisoning: widespread implications of saxitoxin in Florida [Abstract]. In: Xth International Conference on Harmful Algae, 21-26 October 2002, St. Petersburg, FL. St. Petersburg, FL:Florida Institute of Oceanography and Florida Fish and Wildlife Conservation Commission,160.
Landsberg JH, Kiryu Y. 2005. Shrimp Disease. Available: http://www.floridamarine.org/features/view_article.asp?id=25055 [accessed 20 October 2005].
Luckas B, Hummert C, Oshima, Y. 2003. Analytical methods for paralytic shellfish poisons. In: Manual on Harmful Marine Microalgae (Hallegraeff GM, Anderson DM, Cembella AD, eds). Paris:United Nations Educational, Scientific and Cultural Organization, 191-209.
Maclean JL. 1979. Indo-Pacific red tides. In: Toxic Dinoflagellate Blooms (Taylor DL, Seliger HH, eds). New York:Elsevier, 173-178.
Mosher HS, Fuhrmann FA. 1984. Occurrence and origin of tetrodotoxin. In: Seafood Toxins (Ragelis EP, ed). Washington, DC:American Chemical Society, 333-344.
Nakamura M, Oshima Y, Yasumoto T. 1984. Occurrence of saxitoxin in puffer fish. Toxicon 22: 381-385.
Nakashima K, Arakawa O, Taniyama S, Nonaka M, Takatani T, Yamamori K, et al. 2004. Occurrence of saxitoxins as a major toxin in the ovary of a marine puffer Arothron firmamentum. Toxicon 43:207-212.
Negri A, Stirling D, Quilliam M, Blackburn S, Bolch C, Burton I, et al. 2003. Three novel hydroxybenzoate saxitoxin analogues isolated from the dinoflagellate Gymnodinium catenatum. Chem Res Toxicol 16:1029-1033.
Nunez-Vazquez EJ, Yotsu-Yamashita M, Sierra-Beltran AP, Yasumoto T, Ochoa JL. 2000. Toxicities and distribution of tetrodotoxin in the tissues of puffer fish found in the coast of the Baja California Peninsula, Mexico. Toxicon 38:729-734.
Ogura Y. 1971. Fugu (pufferfish) poisoning and the pharmacology of crystalline tetrodotoxin in poisoning. In: Neuropoisons (Simpson LL, ed). New York:Plenum Press, 139-159.
Oshima Y. 1995. Post-column derivatization HPLC method for paralytic shellfish poisons. In: Manual on Harmful Marine Microalgae (Hallegraeff GM, Anderson DM, Cembella AD, eds). Paris:United Nations Educational, Scientific and Cultural Organization, 88-94.
Phlips EJ, Badylak S, Youn S, Kelley K. 2004. The occurrence of potentially toxic dinoflagellates and diatoms in a subtropical lagoon, the Indian River Lagoon, Florida, USA. Harmful Algae 3:39-49.
Powell CL, Doucette GJ. 1999. A receptor binding assay for paralytic shellfish poisoning toxins: recent advances and applications. Nat Toxins 7:393-400.
Quilliam M, Wechsler D, Marcus S, Ruck B, Wekell M, Hawryluk T. 2004. Detection and identification of paralytic shellfish poisoning toxins in Florida pufferfish responsible for incidents of neurologic illness. In: Harmful Algae 2002 (Steidinger KA, Landsberg JH, Tomas CR, Vargo GA, eds). St. Petersburg, FL:Florida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography, and Inter-governmental Oceanographic Commission of United Nations Educational, Scientific and Cultural Organization, 116-118.
Rodrigue DC, Etzel RA, Hall S, de Porras E, Velasquez OH, Tauxe RV, et al. 1990. Lethal paralytic shellfish poisoning in Guatemala. Am Soc Trop Med Hyg 42:267-271.
Ruberu SR, Liu YG, Wong CT, Perera SK, Langlois GW, Doucette GJ, et al. 2003. Receptor binding assay for paralytic shellfish poisoning toxins: optimization and interlaboratory comparison. J AOAC Int 86:737-745.
Sato S, Kodama M, Ogata T, Saitanu K, Furuya M, Hirayama K, et al. 1997. Saxitoxin as a toxic principle of a freshwater puffer, Tetraodon fangi, in Thailand. Toxicon 35:137-140.
Sato S, Ogata T, Borja V, Gonzales C, Fukuyo Y, Kodama M. 2000. Frequent occurrence of paralytic shellfish poisoning toxins as dominant toxins in marine puffer from tropical water. Toxicon 38:1101-1109.
Shipp RL, Yerger RW. 1969a. A new puffer fish, Sphoeroides parvus, from the western Gulf of Mexico, with a key to species of Sphoeroides from the Atlantic and Gulf coasts of the United States. Proc Biol Soc Wash 82:477-488.
Shipp RL, Yerger RW. 1969b. Status, characters, and distribution of the northern and southern puffers of the genus Sphoeroides. Copeia 1969:425-433.
Shumway SE. 1990. A review of the effects of algal blooms on shellfish and aquaculture. J World Aquacult Soc 21:65-104.
Steidinger KA, Landsberg JH, Tomas CR, Burns JW. 1999. Harmful Algal Blooms in Florida. St. Petersburg, FL:Florida's Harmful Algal Bloom Task Force, Florida Marine Research Institute. Available: http://www.floridamarine.org/features/view_article.asp?id=26925 [accessed 16 August 2006].
Steidinger KA, Tester LS, Taylor FJR. 1980. A redescription of Pyrodinium bahamense var. compressa (Bohm) stat. nov. from Pacific red tides. Phycologia 19:329-337.
Tremain DM, Adams DH. 1995. Seasonal variations in species diversity, abundance, and composition of fish communities in the northern Indian River Lagoon, Florida. Bull Mar Sci 57:171-192.
Truby EW. 1997. Preparation of single-celled marine dinoflagellates for electron microscopy. Microsc Res Tech 36:337-340.
Usleber E, Schneider E, Terplan G. 1991. Direct enzyme immunoassay in microtitration plate and test strip format for the detection of saxitoxin in shellfish. Lett Appl Microbiol 13:275-277.
Usup G, Azanza RV. 1998. Physiology and bloom dynamics of the tropical dinoflagellate Pyrodinium bahamense. In: Physiological Ecology of Harmful Algal Blooms (Anderson DM, Cembella AD, Hallegraeff GM, eds). Heidelberg: Springer-Verlag, 81-94.
Vargas-Montero M, Freer E. 2004. Paralytic shellfish poisoning outbreaks in Costa Rica. In: Harmful Algae 2002 (Steidinger KA, Landsberg JH, Tomas CR, Vargo GA, eds). St. Petersburg, FL:Florida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography, and Inter-governmental Oceanographic Commission of United Nations Educational, Scientific and Cultural Organization, 482-484.
Zaman L, Arakawa O, Shimosu A, Onoue Y. 1997. Occurrence of paralytic shellfish poison in Bangladeshi freshwater puffers. Toxicon 35:423-431.
Jan H. Landsberg, (1) Sherwood Hall, (2) Jan N. Johannessen, (3) Kevin D. White, (4) Stephen M. Conrad, (2) Jay P. Abbott, (1) Leanne J. Flewelling, (1) R. William Richardson, (1) Robert W. Dickey, (5) Edward L.E. Jester, (5) Stacey M. Etheridge, (2) Jonathan R. Deeds, (2) Frances M. Van Dolah, (6) Tod A. Leighfield, (6) Yinglin Zou, (7) Clarke G. Beaudry, (4) Ronald A. Benner, (2) Patricia L. Rogers, (2) Paula S. Scott, (1) Kenji Kawabata, (1) Jennifer L. Wolny, (1,8) and Karen A. Steidinger (1,8)
(1) Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, St. Petersburg, Florida, USA; (2) Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, Maryland, USA; (3) Food and Drug Administration, Office of the Commissioner, Rockville, Maryland, USA; (4) Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland, USA; (5) Food and Drug Administration, Center for Food Safety and Applied Nutrition, Gulf Coast Seafood Laboratory, Dauphin Island, Alabama, USA; (6) National Oceanic and Atmospheric Administration, National Ocean Service, Center for Coastal Environmental Health and Biomolecular Research, Charleston, South Carolina, USA; (7) Key Laboratory of Science and Engineering for Marine Ecology and Environment, First Institute of Oceanography, State Oceanic Administration, Qingdao, China; (8) Florida Institute of Oceanography, University of South Florida, St. Petersburg, Florida, USA
Address correspondence to J.H. Landsberg, Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, 100 Eighth Ave. SE, St. Petersburg, FL 33701 USA. Telephone: (727) 896-8626. Fax: (727) 893-9840. E-mail: email@example.com
We thank L. Sebastian, R. Paperno, D. Adams, D. Tremain, S. Fisk, S. Stahl, S. Cook, J. D'Urso, and A. Shurtleff, Florida Fish and Wildlife Conservation Commission (FWC), for technical assistance and D. Bodager and G. Jackow, Florida Department of Health (FDOH), for specimen collection.
Funding or support for this research was provided by the FWC, FDOH, U.S. Food and Drug Administration, Centers for Disease Control and Prevention, and the National Oceanic and Atmospheric Administration (NOAA). This article is a result of research partially funded by the NOAA Coastal Ocean ECOHAB Program under award #NA03NOS4780196 to the FWC (ECOHAB contribution #152).
The authors declare they have no competing financial interests.
Received 11 January 2006; accepted 5 July 2006.
Table 1. Comparison of saxitoxin concentrations ([micro]g STX eq/100 g tissue) in muscle and liver of IRL puffer fish species by ELISA. Muscle Puffer fish species No. Mean [+ or -] SD Maximum Southern 402 938.4 [+ or -] 1,418 14,571 Checkered 105 6.9 [+ or -] 11.4 104.3 Bandtail 9 121.7 [+ or -] 117.9 364.5 Liver Puffer fish species No. Mean [+ or -] SD Maximum Southern 55 265.6 [+ or -] 393 1,443 Checkered 3 20.3 [+ or -] 27.1 51.1 Bandtail 0 -- -- Table 2. Comparison of saxitoxin-like activity levels ([micro]g STX dihydrochloride eq/100 g tissue) by LC-MS in muscle and liver of southern puffer fish (S. nephelus) collected from the IRL after the first SPFP cases in 2002. MBA RBA MNCA Fold Fold Fold Fish Muscle Liver diff Muscle Liver diff Muscle Liver diff 1 5,264 1,034 5.1 4,136 711 5.8 2,294 420 5.5 2 4,697 376 12.5 6,091 304 20.0 1,230 280 4.4 3 2,986 242 12.3 2,433 280 8.7 1,947 160 12.2 4 2,804 203 13.8 1,423 147 9.7 1,100 120 9.2 5 2,564 149 17.2 5,253 297 17.7 844 110 7.7 6 2,153 135 15.9 2,911 173 16.8 790 240 3.3 7 1,970 263 7.5 2,257 142 15.9 750 150 5.0 8 1,216 254 4.8 805 154 5.2 350 140 2.5 9 1,098 221 5.0 1,089 180 6.1 480 110 4.4 10 376 83 4.5 198 16 12.4 630 70 9.0 11 197 149 1.3 231 50 4.6 120 60 2.0 Fold diff indicates fold difference of muscle compared with liver.
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|Author:||Steidinger, Karen A.|
|Publication:||Environmental Health Perspectives|
|Date:||Oct 1, 2006|
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