Sartorius Introduces Sartobind Cassettes.
Sartorius Stedim Biotech (SSB) has expanded its range of single-use membrane chromatography solutions with Sartobind Cassettes. The podlike modular system was developed for commercial applications in both capture and polishing.
The Sartobind cassettes offer the same flow path, bed heights (4 and 8 mm) and void volume ratios as Sartobind capsules, and are compatible with Q, S, STIC PA and phenyl ligands. The new design overcomes the previous 5 L size limitation for capsule formats, expanding the boundaries of membrane chromatography. Multiple cassettes, each with 0.8 L or 1.6 L membrane volume, can be set up in three different stainless steel holders with maximum membrane volumes of 20, 50 or 100 L, respectively.
Data demonstrates direct scalability from the 3 mL Sartobind nano capsule to 20.8 L (13 cassettes) in the Pilot Filter Holder. Pressure-flow performance and the shape of breakthrough curves are identical to the smaller capsule sizes, independent of the number of cassettes used. According to the company, set up can be accomplished within minutes, even at manufacturing scale, whether for capture applications or for flow-through removal of process contaminants.
The capture of large proteins such as viruses and virus-like particles (VLPs), protein conjugates and blood factors plays a key role in modern bioprocessing. The Sartobind cassette system now allows large-scale bind-and-elute membrane chromatography for such targets.
"For the first time, the high binding capacity of membrane adsorbers can be used at almost any scale in commercial vaccine manufacture. Now, a 200 L Q anion exchange column for adenovirus capture can be easily replaced by a 20 L Q adsorber cassette system," said Dr. Stefan Fischer-Fruhholz, membrane chromatography expert at Sartorius Stedim Biotech.
More info: www.sartorius.com
Caption: Sartorius Cassettes