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Salicinium--disrupting anaerobic glycolosis and improving GcMAF immune response.

Many of my patients have benefitted from Salicinium[R], a glyco benzaldehyde that is a viable immune system enhancer. Salicinium has a unique mechanism of action, which I will explain throughout this article. I will also provide a case study, where a male patient, WW, received his initial treatment with Salicinium in May of 2014 for diagnosis of prostate cancer. This previous prostate diagnosis was alleviated as was determined by his oncologist through biopsy. As noted in previous articles in the Townsend Letter, which appeared in the August/September 2013, 2014, 2015, and 2016 issues, the efficacy of Salicinium is well documented. It has been my experience and that of other doctors who have used it in thousands of patients that it is highly effective as an immune-enhancing substance. Much of the evidence supporting Salicinium's effectiveness is derived from its extensive use in clinical practice as well as from tests that labs have performed on its mechanism of action.

Salicinium is a natural glyco benzaldehyde. It is the smallest and simplest of all known sugar/benzaldehydes in nature. There have been other benzaldehydes used in cancer such as glyco benzylidene extracted from the Japanese fig, (1) but this molecule was so difficult to extract it had to be synthesized. The synthesized version could not be made to last more than 36 hours and so fell out of favor in most of the world. The above study shows the superiority of a glyco benzaldehyde molecule such as Salicinium being taken up by both bacteria and anaerobic mammalian cells.

The goal of this article is to demonstrate the ability of Salicinium to boost the immune system, reducing the bio-chemical actions that are generating subnormal cell structures. These bio-chemical actions which are affected by Salicinium were explained well in the book Life on the Edge:
Biomolecules such as DNA or enzymes are made of fundamental particles
like protons and electrons whose interactions are governed by quantum
mechanics and... must ultimately depend on quantum mechanical forces
governing electrons and protons just as the operation of your car or
your toaster depends, ultimately, on quantum mechanical forces
governing electrons." (2)


What the author is referring to is changing the bio-electrical interaction of the cellular pathway.

Salicinium's Mechanisms of Action

Our cells obtain oxygen through a process known as respiration, which takes place in the mitochondria, the energy-generating furnace of our cells. Because this process is dependent upon oxygen it's called an aerobic process. Through respiration, the mitochondria produce ATP (adenosine triphosphate), the energy molecule needed to live. (3)

Dr. Otto Warburg discovered that by lowering oxygen levels of normal cells by 35%, they can continue to live without respiration. (4,5) All the cells in our body can use this anaerobic "without oxygen" process to help them survive a short time of stress. However, should the cell suffer a long-term stress, it becomes an aerotolerant anaerobe (6,7) and continues to live through a process called fermentation even if oxygen is present. This is problematic, because anaerobic, long-term, fermenting cells become cancerous.

These fermenting cells generate only 5% as much ATP as when they lived on oxygen. Instead of living with oxygen, they ferment simple sugars. When compared with normal cells, fermenting cells have many more sugar receptors on their surface once they become obligated to fermentation. (8,9)

Based on the research of Dr. Otto Warburg, it has long been presumed that simply adding more oxygen back into cells through the use of antioxidants such as high-dose vitamin C, high-dose vitamin E, many vitamins, minerals, and hundreds of herbs, plants, nostrums, and even oxygen itself (through a hyperbaric chamber) will cause the cell to become healthy once more by returning to an aerobic state. However, this is not the case, and the reason involves an enzyme called nagalase (alpha-N-acetylgalactosaminidase). Nagalase forms a protective coating around the anaerobic cell, stopping the body's immune system from recognizing there is a problem. (8) Nagalase also blocks GcMAF, a regulatory protein that enhances the immune system through its effects on macrophages. (10) Healthy people have high levels of GcMAF whereas people with weak immune systems have lower levels of this protein. (10)

The normal cells in our body are covered with a carbohydrate coating called glycocalyx. This glycoprotein coating has many purposes, but seemingly its main function is for "self recognition." It may be harmed by various things, but the worst is hypoxia. Hypoxia is the key to cancer cell survival found by Warburg. When the glycocalyx is forced into hypoxia the glycoprotein coating quickly undergoes a rapid change and becomes nagalase, the glycoprotein of hypoxia. However, if the timeline for recovery runs out it remains as the protective, non-recognition defense mechanism of cancer. This non-recovery timeline is unknown as every different type of cell has a different time-life. (11)

Despite the fact that it is hypoxia that triggers the change of the glycoprotein coating into nagalase, it is not oxygen which stops obligated, nagalase-producing pathogens. Pathogens are exactly the opposite in that providing oxygen is not effective. While it is true that a normal cell living by anaerobiasis can return to normal with the return of respiration, (9,10) an aerotolerant anaerobe cannot, due to injured mitochondria.

The glyco benzaldehyde works by changing the bio-electrical interaction capacity of the glycolytic pathway. In other words, it is the source of a pH-changing, bio-chemical/electrical reaction naturally delivered to the inside of a fermenting cell. Once the bio-chemical interaction of a cell is changed, the immune system can recognize the pathogen and destroy it. (2,12) Benzaldehyde reacts with most pathogens in a process similar to redox/antioxidant activities, changing the electron footprint that will inevitably change the pH of the cell. This change brings about a loss in mitochondrial membrane potential. (13)

Because benzaldehyde is attached to a sugar molecule, it is readily accepted into the cell. It accomplishes this by entering any fermenting anaerobe through the glucose transporter (GLUT) pathway. GLUTs are present in all cell types, but cancer cells often overexpress GLUTs. (14) In the GLUT pathway, benzaldehyde is met immediately by the enzyme hexokinase Il (HK2) and, through enzymatic reaction with ATP, is changed to glucose 6-phosphate-benzaldehyde (G 6-p-b). (3) G 6-p-b, again through a further enzymatic reaction and another investment of ATP, becomes fructose 1 6-bisphosphate-benzaldehyde (FBP-b). (15-18) Most of the glucose and fructose that "feed" a cell are converted into FBP. Through this process, benzaldehyde is able to enter the cell clandestinely.

To understand the mechanism behind Salicinium's efficacy, first, one needs to recognize that it is a glyco benzaldehyde that has irreversible modifying effects on HK2, G 6-P, F 6-p, and FBP, by changing their electrical potential. (19) Sugar is the taxi cab and benzaldehyde is the passenger.

This is called targeted therapy. In the article, "An Overview of Targeted Cancer Therapy," it states: "Targeted therapy is gaining importance due to its specificity towards cancer cells while sparing toxicity to off-target cells. The scope of this review involves the various strategies involved in targeted therapy like monoclonal antibodies, prodrug, small molecule inhibitors, and nano-particulate conjugates." The authors go on to write:
Targeted therapy involves developing drugs that block cancer cell
proliferation, promote cell cycle regulation, or induce apoptosis [a
process by which the body removes damaged cells] or autophagy [the
natural destruction of cells in order to remove unnecessary or
dysfunctional components] and delivery of toxic substances specifically
to cancer cells to destroy them. Targeted therapy involves the use of
monoclonal antibodies or oral small drugs.


Furthermore, the authors state, "The small drugs usually are targeted against specific molecular targets which are important for cancer cell proliferation or metastasis or angiogenesis." (20)

Salicinium falls into the category of a nano-particulate conjugate and therefore is a targeted therapy, which induces apoptosis through its effects. (21)

Salicinium is a glycome (sugar) conjugated (attached) to benzaldehyde which is toxic to the glycolytic pathway upon entry into any fermenting cell. There are many conjugate references one may look up online, both in the field of pharmacokinetic and pharmacodynamic properties, especially the field of chemotherapy. (22,23)

Salicinium's Effects on Nagalase

An important point to keep in mind is the role that the enzyme nagalase plays in protecting anaerobic cells. Salicinium's mechanism of action involves in part its ability to cause unhealthy cells to release their nagalase coating, thus allowing the immune system to rejuvenate. We have seen this in reports from labs that we use to test nagalase levels in patients given Salicinium. After using Salicinium, nagalase levels drop in these patients while concurrently immunoglobulins or the white blood cell count rise.

The glyco benzaldehyde Salicinium causes cells to release their nagalase coating in part through its effects on the enzyme pyruvate kinase 2 (PK2). According to the article "Glycolysis: Process of Glucose Utilization and Homeostasis," the PK enzyme is inhibited by ATP. That article states:
Most tissues express either the PK1 or PK2. PK1 is found in many normal
differentiated tissues, whereas PK2 is expressed in most proliferating
cells, including all cancer cell lines and tumors tested to date.
Although PK1 and PK2 are highly similar in amino acid sequence they
have different catalytic and regulatory properties. PK1 has high
constitutive enzymatic activity. In contrast, PK2 is much less active
but is allosterically activated by the upstream glycolytic metabolite
fructose 1, 6-bisphosphate (FBP). (18)


In other words, the downstream PK2 enzyme is first pushed away or held back by ATP and then FBP activates it. (19) As we mentioned earlier, Salicinium, combined with a sugar, is converted into an unnatural FBP-b.

Now we come to the crux of how to control the metabolism of any anaerobic fermenting cell. Studies show that the endoplasmic reticulum (a type of organelle that plays an important role in transporting sugars and proteins across the cell membrane) and the hexosamine pathway, which I described earlier in this article, are involved in apoptosis. (24-30) Salicinium's mechanism also involves the endoplasmic reticulum and the HK2 pathway. Due to the glyco benzaldehyde, there is no longer an upstream glycolytic metabolite with a bio-chemical charge to match the low energy PK2 enzyme. The FBP metabolite was irreversibly changed when the HK2 enzyme embraced the Salicinium-sugar molecule upon its entry through the GLUT pore. PK2, having neither the matching energy, nor the time, is halted. The minimal glycolytic-energy function of the anaerobic cell becomes completely dysfunctional and dysregulated and the nagalase protective coating cannot be maintained and is released. This ultimately results in tumor necrosis. (31)

These results suggest that Salicinium induces endoplasmic reticulum stress in HK-2 mediated cells, which is in turn involved in apoptosis or autophagy. The glycolytic pathway and its relationship to the endoplasmic reticulum are easily understood by referring to the pdf file available at the website noted in reference 3.

Furthermore, a paper by Cheico and colleagues titled, "Quantitative Histochemistry of benzaldehyde dehydrogenase in hepatocellular carcinomas of vinyl chloride-treated rats," indisputedly shows that the active component of Salicinium (benzaldehyde) attaches to nicotinamide adenine dinucleotide phosphate (NADP). (19) NADP plays an important role in the oxidation-reduction involved in protecting against the toxicity of reactive oxygen species (ROS). This means Salicinium affects every single reaction in the glycolytic pathway as soon as it is irreversibly attached to HK-II upon entry, and NADP is where "the buck stops."

Phagocytosis Is What Ultimately Destroys These Now Unprotected Cells

With the Salicinium-triggered release of the enzyme nagalase from the surface of any fermenting anaerobic cell, the anaerobe becomes immediately discernible to innate Immune responses. When discovery occurs, natural killer (NK) cells are rallied by the now unimpeded immune system to move in and phagocytize the obvious dysfunctioning anaerobic cell.

In the case of a circulating tumor cell (CTC) or cancer stem cell (CSC), when phagocytosis commences, the injuring of the surface of the cell immediately brings on the signal for the voltage dependent anion channel (VDAC). In cancer cells, the HK2 enzyme is functional with VDAC, which is an outer mitochondrial membrane protein that integrates cellular energy metabolism through the regulation of the influx and efflux of metabolites and ions. (32) Important: Notice the terminology "Voltage Dependent Anion Channel." This is an activity governed by electrons and protons and the importance of electrons and protons was mentioned earlier. VDAC is overexpressed in many cancer cells and it is thought to increase cancer cell survival. (32,33)

Also during phagocytosis, a protease known as caspase-9 is released into the cytoplasm causing apoptosis. (30,34) This same reaction happens exactly the same way when a normal cell is injured. In years past there has been conjecture as to the demise of fermenting cells brought on by Salicinium. Did it kill the cancer cell as chemotherapy does or did it create an environment whereby cancer cells could be killed? The following Figure 1 showing Salicinium (liquid version--Orasal[TM]) tested against six different lines of cells shows undeniably Salicinium disrupts the metabolic activity of the cell allowing the cell to self destruct and be phagocytized. This disruption would bring on a concomitant release of its nagalase coating, leaving the cell unprotected from outside influences and no possibility of correction from the inside. Salicinium does not "kill" fermenting cells; it exposes them for what they are.

Salicinium's Effects on Glycolysis

Glycolysis is the process by which the body produces the energy molecule ATP along with NAD and NADH. It occurs in the cells of all living organisms. The enzyme HK, mentioned earlier, is the first enzyme of the glycolysis pathway.

At the point where Salicinium enters the cell, the cytoplasm of the anaerobe has just invested ALL the energy (ATP) available to maintain its function. In the absence of Salicinium, the investment of two ATP would normally go forward to produce four ATP, two of which are recycled back into the glycolytic cycle and two of which are used to produce pyruvate, which is essential for cellular respiration in the mitochondria.

However, in the presence of the glyco benzaldehyde Salicinium, this process is altered. As mentioned earlier, Salicinium changes electrical potential. All cells exist through the movement of electrons and protons generating life. The potential of every cell is in constant flux to reach its full chemical, electrical, or bio-chemical potential. (24,25) By virtue of its ability to add or take away an electron or proton to or from a cell, Salicinium halts the glycolytic pathway, disrupting NAD, NADH, and ATP development. Because only two ATPs are available at this stage, just two molecules of Salicinium are required to shutdown the glycolytic pathway.

Without glycolysis the dysregulated anaerobic cell can no longer maintain the enzymatic coating of nagalase, the enzyme blocking the development of GcMAF. Without inhibition by nagalase, the immune system once again can resume the function of joining GcMAF to a de-activated macrophage, becoming NK cells that accomplish what nature intended all along--enhancing immunity. This has been well brought out in past Salicinium articles in the Townsend Letter using graphs from BioFocus Laboratory in Germany and RGCC Laboratory in Greece.
Figure 1: Salicinium's Effect on Cancer Cells

   Cell Mortality %

Breast Ca.         83%
Colon Ca.          85%
Prostate Ca.       81%
Lung Ca.           70%
Ovarian Ca.        68%
Cervical Ca.       60%

A higher concentration of caspase-9, the lower the level of p53 protein
and Bcl-2. Conversely, the lower the concentration of caspase-9, the
greater the level of p53 protein and Bcl-2, both related to the MDR.
Cell death of more than 50% result in good response via the
caspase-independent apoptosis. NOTE: the results correspond only to the
samples tested.

Note: Table made from bar graph.


Nagalase can easily be measured in the blood and falls concurrently as the anaerobic cells are necrosing. By the time the nagalase level reaches well into the normal range (.32-.95 nmol/ml/min) tumor markers will also be returning to normal. PET scans will show no uptake and imaging will be clear or may still show some tumor scar, which the body will break down over time.

Case Study

In the introduction to this article, I mentioned the case of a man (WW) who received his initial treatment with Salicinium in May of 2014 for diagnosis of prostate cancer. This previous prostate diagnosis was alleviated as was determined by his oncologist through biopsy.

This case continues with a recent diagnosis of bone metastasis missed on initial oncology visits. The test (PSMA PET/CT) was obtained through University of California, Davis, which noted small cellular uptake in the pelvic, rib, and scapula areas. All are Prostate 4+ metastatic primary sites, and being of a slower metabolizing tissue had not been completely resolved. Patient returned to continue oral Salicinium and IV infusions with a continued treatment plan for the metastatic diagnosis.

Testing

For Improved immune response and reactionary immune globulins, physiological targeting of Salicinium has been noted in laboratory testing with BioFocus of Germany and RGCC of Greece as well as nagalase testing from the European Lab of Nutrients in the Netherlands. All of these tests are beneficial for the screening of pathogenic cell development and reducing the development of circulating tumor and stem cells. Further viral testing should be done to rule out co-morbidities

Treatment

Commitment of 15 days IV infusion with oral on non IV days, then continuance of oral until testing shows clear. Side effects may be localized fever with improved immune function.

References:

(1.) Pettersen EO, et al. 4,6-Benzylidene-D-glucose, a benzaldehyde derivative that inhibits protein synthesis but not mitosis of NHIK 3025 cells. Cancer Res. 1985 May;45(5):2085-91.

(2.) John Joe McFadden and Jim Al-Khalili. Life on the Edge, Broadway Books, pg. 20.

(3.) Medh JD. The 3 stages of glycolysis, www.csun.edu/~jm77307/Glycolysis.pdf Accessed June 26, 2017.

(4.) Warburg O. The Prime Cause and Prevention of Cancer. Lecture to Nobel Laureates, June 30, 1966.

(5.) Warburg O. On the Origin of Cancer Cells. Science. 24 February 1956;123(3191):309-14.

(6.) Biology Online. http://www.biology-online.org/dictionary/Aerotolerant Accessed June 26, 2017.

(7.) Brown CM. Salicinium: A powerful biological response modifier in cancer. Townsend Letter. August-September 2015.

(8.) Thyer L, et al. GC protein-derived macrophage-activating factor decreases a-N-acetylgalactosaminidase levels in advanced cancer patients. Oncoimmunology. 2013 Aug 1;2(8):e25769.

(9.) Vazquez A, et al. Catabolic efficiency of aerobic glycolysis: the Warburg effect revisited. BMC Syst Biol. 2010 May 6;4:58.

(10.) Thyer L, et al. A Novel Role for a Major Component of the Vitamin D Axis: Vitamin D Binding Protein-Derived Macrophage Activating Factor Induces Human Breast Cancer Cell Apoptosis through Stimulation of Macrophages. Nutrients. 2013 Jul; 5(7): 2577-89.

(11.) Lahir YH. Understanding the basic role of glycocalyx during cancer. Journal of Radiation and Cancer Research. 2016;7(3):79-84.

(12.) Jerry Tennant, MD. Healing is Voltage, pgs. 32, 48, 297-98.

(13.) Jang TY, et al. Benzaldehyde suppresses murine allergic asthma and rhinitis. Int Immunophormacol. 2014 Oct;22(2):444-50.

(14.) Medina RA, Owen Gl. Glucose transporters: expression, regulation and cancer. Biol Res. 2002;35(1):9-26.

(15.) Salway JG. Medical Biochemistry at a Glance. Third Edition 2012, pg. 42.

(16.) Ibid-Opening front pages- Glycolysis Pathway

(17.) Jang M, et al. Cancer cell metabolism: implications for therapeutic targets. Exp Mol Med. 2013 Oct 4;45:e45.

(18.) The Medical Biochemistry Page. http://Themedicalbiochemistrypage.org/glycolysis.php Accessed June 24, 2017.

(19.) Chieco P, et al. Quantitative histochemistry of benzaldehyde dehydrogenase in hepatocellular carcinomas of vinyl chloride-treated rats. J Histochem Cytochem. 1986 Feb;34(2):151-8.

(20.) Padma VV. An overview of targeted cancer therapy. BioMedicine. December 2015;5(4): 1-6.

(21.) Lin CF, et al. Synthesis and anticancer activity of benzyloxybenzaldehyde derivatives against HL-60 cells. Bioorg Med Chem. 2005 Mar 1;13(5):1537-44.

(22.) Cartel L, et al. The role of conjugation processes and linking agents in the preparation of molecular/particulate conjugates: A review. S.T.P. Pharma Sciences. 1999 July;(4):307-19.

(23.) Cartel L, et al. From conventional to stealth liposomes: a new Frontier in cancer chemotherapy. J Chemother. 2004 Nov;16 Suppl 4:94-7.

(24.) Zhu S, et al. The endoplasmic reticulum stress response is involved in apoptosis induced by aloe-emodin in HK-2 cells. Food Chem Toxicol. 2012 Mar;50(3-4):1149-58.

(25.) Marchi S, et al. Oncogenic and oncosuppressive signal transduction at mitochondria-associated endoplasmic reticulum membranes. Mol Cell Oncol. 2014 Oct 29;1(2):e956469.

(26.) Gitenay D, et al. Glucose metabolism and hexosamine pathway regulate oncogene-induced senescence. Cell Death Dis. 2014 Feb 27;5:e1089.

(27.) Hou X, et al. PERK silence inhibits glioma cell growth under low glucose stress by blockage of p-AKT and subsequent HK2's mitochondria translocation. Sci Rep. 2015 Mar 12;5:9065.

(28.) Patergnani S, et al. Mitochondria-Associated Endoplasmic Reticulum Membranes Microenvironment: Targeting Autophagic and Apoptotic Pathways in Cancer Therapy. Front Oncol. 2015 Jul 27;5:173.

(29.) Kato H, Nishitoh H. Stress responses from the endoplasmic reticulum in cancer. Front Oncol. 2015 Apr 20:5:93.

(30.) Druskovic M, et al. Over expression of Caspase-9 Triggers Its Activation and Apoptosis in Vitro. Croat Med J. 2006 Dec;47(6):832-40.

(31.) Pettersen EO, et al. Antitumour effect of benzylidene-glucose (BG) in rats with chemically induced hepatocellular carcinoma. Anticancer Res. 1986 Mar-Apr;147-52.

(32.) Hoa C, et al. Mild Alkalization Acutely Triggers the Warburg Effect by Enhancing Hexokinase Activity via Voltage-Dependent Anion Channel Binding. PLoS One. 2016; 11(8):e0159529.

(33.) Shoshan-Barmatz V, et al. The mitochondrial voltage-dependent anion channel 1 in tumor cells. Biochim Biophys Acta. 2015 Oct;1848(10 Pt B):2547-75.

(34.) Canto C, et al. NAD(+) Metabolism and the Control of Energy Homeostasis: A Balancing Act between Mitochondria and the Nucleus. Cell Metab. 2015 Jul 7;22(1):31-53.

[ILLUSTRATION OMITTED]

by Virginia Osborne, ND

Virginia Osborne, ND, graduated from the accredited National College of Naturopathic Medicine (NCNM) in Portland, Oregon, following a career as an RN in critical, emergency, and cardiac experience. She is an IV therapy academic instructor and clinical department supervisor at NCNM/Natural Health Centers. She is a co-founder of International IV Nutritional Therapy for Physicians; she lectures and develops workshops on IV nutrition, anti-aging, chelation, and clinical emergency medicine; and she is the co-author of Parenteral Micronutrient Therapy. Her private practice is in Grass Valley, California, with a focus on IV treatment modalities for environmentally induced diseases. She can be reached at IVnutritionaltherapy.com.
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Title Annotation:Gc protein-derived macrophage activating factor
Author:Osborne, Virginia
Publication:Townsend Letter
Date:Aug 1, 2017
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