Printer Friendly

Saffron: a concise review of researches.

Introduction

Saffron, Crocus sativus L., is an important crop cultivated as the source of its spice for at least 3,500 years. Saffron is a perennial spice species of Iridaceae Family and has been spread out in Mediterranean and west of Asia from 10 west to 80 east degrees of geographical longitude, as well as from 30 to 50 north degrees of geographical latitude and up to 1000 meters from sea level [27]. Dried stigmas of saffron flowers compose the most expensive spice which has been valuable since ancient times for its odoriferous, coloring, and medicinal properties [38].

It has been propagated and still continues to be propagated vegetatively. There is a supposition that saffron as a clone can be scarcely changed genetically and its improvement is hardly possible through clonal selection [9,37].

The name saffron is commonly used to refer both to the spice and the plant itself. Some archaeological and historical studies indicate that domestication of saffron dates back to 2,000-1,500 years BC [19]. Saffron is currently being cultivated more or less intensively in Iran, India, Greece, Spain, Italy, Turkey, France, Switzerland, Israel, Pakistan, Azerbaijan, China, Egypt, United Arab Emirates, Japan, Afghanistan, Iraq and recently Australia (Tasmania) [35]. The name 'saffron' is derived from Arabic za-faran which means 'be yellow' [44].

Saffron leaves (with producing about 1.5 t dry matter each year) can provide forage for about 160000 heads of cattle. Saffron petal is one of the by-products of fields that the amount of this byproduct is more than 10000 t each year. Nowadays, the only usage of saffron petals is dye extraction, which is not flourished yet [14].

This review focuses on botany, propagation, uses and production of saffron.

Taxonomy and Morphology:

Saffron is classified into Magnoliophyta division, class Liliopsida and order Asparagales. It is a member of the Iridaceae family and the Crocus L. genus. Iridaceae family includes about 60 genera and 1,500 species. The plants in this family are herbs

with rhizomes, corms or bulbs. The Crocus genus includes approximately 80 species worlwide. Among these, the cultivated species saffron is well known with its high economic value [16]. At present about 8 taxa are recognized in Iran [18].

Saffron is produced from the dried styles of Crocus sativus L. (Iridaceae) which is unknown as a wild plant, representing a sterile triploid derived from the naturally occurring diploid C. cartwrightianus Herbert. These belong to subgenus Crocus series Crocus which constitutes 9 species: C. cartwrightianus and its derivative Crocus sativus, C. moabiticus, C. oreocreticus, C. pallasii, C. thomasii, C. hadriaticus., C. asumaniae and C. mathewii. The taxonomy of these species and their infraspecific taxa is presented, together with their distribution, ecology and phenology; full descriptions and chromosome counts are provided and there is a key to their identification. The genus Crocus, a member of the large family Iridaceae, comprises some 85 species having an Old World distribution, primarily in Mediterranean Europe and western Asia. Phytogeographically, the majority of species occur within the Mediterranean floristic region, extending eastwards into the Irano- Turanian region; both of these areas are characterized by cool to cold winters with autumn-winterspring precipitation and warm summers with very little rainfall; the latter region experiences much colder winters and generally less rainfall. The genus Crocus is well adapted to such conditions, the plants being in active growth from autumn to late spring and surviving the summer drought below ground by means of a compact corm. Many species commence their above-ground growth at the onset of autumn rains and flower almost immediately; some of these produce their leaves and flowers concurrently, or nearly so, while others bloom without leaves and delay their leaf production until the onset of warmer weather, usually in spring. These physiological characteristics, together with cytological information and morphological features of the corm tunics, bracts, bracteols, leaves, flowers and seed, have been used to divide the genus into a hierarchy of subgenera, sections and series, and to define the species within those infrageneric groupings [31].

Saffron is sterile and has fleshy corms. The corms covered by tunics and consist of nodes and are internally made up of starch-containing parenchyma cells. A corm survives for only one season, reproducing via division into "cormlets" that eventually give rise to new plants. Each corm produces six to nine leaves. The photosynthetic activity of the leaves during the early winter and the early spring months contributes to the formation of the replacement corms at the base of the shoots. Leaves are grass-like. The plant shows no aboveground organs or roots during late spring and most of summer.

Flowers autumnal, fragrant, 1-4, deep lilacpurple with darker veins and a darker violet stain in the throat; throat white or lilac, pubescent. Prophyll present. Bract and bracteole present, very unequal, white, membranous with long tapering, rather flaccid tips. Perianth tube 4-5(-8) cm long; segments subequal, 3.5-5 cm long, 1-2 cm wide, oblanceolate or obovate, obtuse. Filaments 7-11 mm long, purplish, glabrous; anthers 15-20 mm long, yellow. Style divided into 3 deep red clavate branches, each branch 25-32 mm long, much exceeding the anthers and at least half the length of the perianth segments, arising at a point well below the base of the anthers in the throat of the flower. Capsules and seeds rarely produced (a triploid of low fertility). 3n=24 [31].

Saffron corms produce both fibrous roots and contractile roots. The fibrous roots emerge from a single ring at the base of the corms. These roots are straight and thin (about 1mm in diameter). The contractile root has the appearance of a tuber organ, very large and whitish. This type of roots enable corms to dig into the ground, so corms rest in optimum depth and position in the soil [6].

Propagation:

Grilli Caiola and Canini [20] study on saffron, Crocus sativus and the diploid species C. cartwrightianus, C. thomasii, C. hadriaticus. When saffron originated is still open to dispute. It has been widely known since the pre-Hellenic and Hellenic periods, but it is impossible to detect if was C. sativus or other Crocus species such as C. cartwrightianus. Concerning the site origin the research indicates two possible sites: one in Greece in the Mediterranean area, the other at East in Turkey-Iran-India. In both areas, records and place names connected with various species of Crocus constitute an important information source for the presence of saffron. Cytological, DNA, and reproductive studies on the allied species of C. sativus such as C. cartwrightianus, C. thomasii, C. hadriaticus, indicate a more likely parent of saffron may be C. cartwrightianus or C. thomasii. Both these species are diploid with a karyotype similar to saffron. In addition, their pollen can fertilize the egg cell of saffron, giving rise to seeds which are viable, germinate and form new corms. Thus saffron can originate through fertilization of a normal reduced egg cell with an unreduced male gamete of the same Crocus species or by crossing between an egg cell and the male unreduced gamete of another species.

The saffron is a sterile geophyte that produces annual renewal corms and is propagated only by them [32]. The physical properties of saffron corms are prerequisite to designing and developing harvesting, handling, sizing and sowing equipments of corms. [21].

Cytological studies indicate that saffron is a sterile triploid (2n=3x=24) plant [4,32]. The origin of saffron by allopolyploidy seems more probable considering the recent data on its karyotype and molecular biology [20]. Many authors have hypothesized the origin of saffron by autotriploid [4,17,33].

On the basis of overall length and centromeric position, the somatic chromosomes could be assembled in seven triplets, one pair and one single chromosome. The karyotype consists of a series of chromosome triplets with the exception of one group of three chromosomes of which one is different from the others. Because of this unique chromosome in saffron genotype, selfing can be excluded [5]. Since Crocus sativus is sterile, the presence of this chromosome may be explained by the existence of chromosomal polymorphism at diploid level in the progenitors, most probably C. thomasii or C. cartwrightianus [4,19,31].

Conventional propagation methods are very slow and propagation by tissue culture represents an important potential to effectively propagate it. Ding et al. [10,11] successfully regenerated callus and intact plantlets from corm explants. Homes et al. [23] produced microcorms on corm explants. Sharafzadeh and Khosh Khui [42] produced microcorms on corm explants and leaves were regenerated by subculturing of microcorms. Ovary wall explants gave the best response, with stigma and style-type structures regenerating from the explants [7]. Corms of C. sativus were induced on shoot explants and from callus [34]. Style and perianth explants produced stigma-like structures [12]. Embryogenic callus of C. sativus was initiated from shoot meristems by Karamian and Ebrahimzadeh [28]. Non-embryogenic and embryogenic callus were produced by Darvishi et al. [8].

Uses of Saffron:

Saffron is commonly used in medicine, as well as dye and spice in food industry. Saffron has been also used as a drug to treat various human health conditions [3].

Compounds considered pharmacologically active and important are volatile agents (e.g. safranal), bitter principles (e.g. picrocrocin) and dye materials (e.g. crocetin and its glycosidic, crocin) [41].

The chemical components of saffron include the coloured carotenoids, crocin, crocetin and monoterpene aldehytes, picrocrocin and saffranol [1,13,30,43].

In medicine, it is used as an antispasmodic, eupeptic, gingival sedative, anticatarrhal, nerve sedative, carminative, diaphoteric, expectorant, stimulant, stomachic, aphrodisiac and emmenagogue [41]. Besides, it has anticonvulsant [25], antidepressant [24], anti-inflammatory [26] and antitumour [13] effects.

Reserchers showed that saffron has a protective effect on chromosomal damage [39,40]. It is an antioxidant and detoxifying spice [39]. Crocetin and crocin, active constituents of saffron, help memory recovery [45]. Crocin is also an antioxidant help overcoming oxidative stress in neurons [36]. An antidepressant effect of saffron petal and stigma in mice was showed [29].

Production of Saffron:

The world's total production of dried saffron is estimated around 300 tons per year [15]. Iran produces more than 90 percent of the world's total production of saffron. More than 92 percent of Iranian saffron is cultivated in Khorasan province with about 210 Tons Annual Production. The Kashmir region in India produces between 8 to 10 tons mostly dedicated to India's self-consumption. Greek production is 4 to 6 tons per year. Morocco produces between 0.8 and 1 ton. Saffron production has decreased rapidly in many traditionally producing countries, and is abandoned in others such as England and Germany. Spain was used to be the traditional world leader and most reputed saffron producer for centuries in areas of La Mancha and Teruel. Nowadays, the production is only about 0.30.5 tons. Productions of Italy (Sardinia, Aquila, Cascia) 100kg; Turkey (Safranbolu) 10kg; France (Gatinais, Quercy) 4-5kg; and Switzerland (Mund) 1kg are nearly insignificant. All saffron producers in the European Union, as well as in Turkey, suffer from increasing labour costs [16].

Despite wide use of this unique spice, it is highly priced due to high demand and low supply. Saffron prices at wholesale and retail rates range from US$1100- US$11,000 per kilogram. In Western countries, the average retail price is US$2200 per kilogram [22]. It is considered to be the highest priced spice in the world [44]. The main reason for its great cost is that saffron is still propagated by human help. The cultivation processes, such as planting, flower harvesting and separation of the stigmas are carried out manually. Each saffron flower has three stigmata and one stigma weights about 2mg. It takes 150,000-200,000 flowers and over 400 h of hand labor to produce 1kg saffron stigma [38]. 75,000 blossoms or 225,000 hand-picked stigmas are required to make a single pound of saffron, which explains why it is the world's most expensive spice [2].

It has been shown that some practices such as mycorrhiza and biohumus were effective for improved saffron cultivation [2].

That is why there are some differences in quality between saffron of different origins and the subsequent fluctuation of prices. Its high value makes saffron the object of adulteration. The addition of artificial colorants is one of the most common means of deception. Such a practice is expected to improve the appearance of the dried stigmas or even to give rise to the coloring strength of the aqueous extract.

Other methods include mixing in extraneous substances like beet, pomegranate fibers, red-dyed silk fibers, or the saffron's tasteless and odorless yellow stamens, and submerging saffron fibers with viscid substances like honey or vegetable oil. Saffron consumption is rising but production is not enough to meet this demand. In order to ensure the future of saffron crop, it is necessary to improve cultivation techniques. The production should be increased due to rising in utilization of saffron as natural product through worldwide.

References

[1.] Abdullaev, F.I., 1993. Biological effects of saffron. Biofactors, 4: 83-86.

[2.] Aytekin, A. and A.O. Acikgoz, 2008. Hormone and microorganism treatments in the cultivation of saffron (Crocus Sativus L.) Plants. Molecules, 13: 1135-1146.

[3.] Basker, D. and M. Negbi, 1983. Uses of saffron. Econ. Bot., 3: 228-236.

[4.] Brighton, C.A., 1977. Cytology of Crocus sativus and its Allies (Iridaceae). Plant Syst. Evol., 128: 137-157.

[5.] Chichiricco, G., 1984. Karyotype and meiotic behaviour of the triploid Crocus sativus L. Caryologia, 37: 233-239.

[6.] Chio-Sang, T., 1996. Effect of planting depth and existence of tunic on growth and flowering in Freesia forcing. J. Korean Hort. Sci., 37: 577581.

[7.] Choob, V.V., T.A. Vlassova and R.G. Butenko, 1994. Callusogenesis and morphogenesis in generative organ culture of the spring flowering species of Crocus L. Russ J. Plant Physiol., 41: 712-716.

[8.] Darvishi, E., R. Zarghami, C.A. Mishani and M. Omidi, 2007. Effects of different hormone treatments on non-embryogenic and embryogenic callus induction and time-term enzyme treatments on number and viability of isolated protoplasts in saffron (Crocus sativus L.). Acta Hort., 739: 279-284.

[9.] Dhar, A.K., R. Sapru, and K. Rekha, 1988. Studies on saffron in Kashmir. 1. Variation in natural population and its cytological behavior. Crop Improvement, 15: 48-52.

[10.] Ding, B., S.H. Bai, Y. Wu and X.P. Fang, 1981. Induction of callus and regeneration of plantlets from corms of Crocus sativus L. Acta. Bot. Sin., 23: 419-420.

[11.] Ding, B., S.H. Bai, Y. Wu and B.K Wang 1979. Preliminary report on tissue culture of corms of Crocus sativus. Acta. Bot. Sin., 21: 387.

[12.] Ebrahimzadeh, R.T. and R. Karamian, 2000. In vitro production of floral buds in stigma-like structures on floral organs of Crocus sativus L. Pak. J. Bot., 32: 141-150.

[13.] Escribano, J., G.L. Alonso, M. CocaPrados and J.A. Fernandez, 1996. Crocin, safranal and picrocrocin from saffron (Crocus sativus L.) inhibit the growth of human cancer cells in vitro. Cancer Lett., 100: 23-30.

[14.] Eskandari-Torbaghan, M., R. Abbasi-Ali Kamar and R. Astarei Ali, 2007. Effect of saffron (crocus sativus L.) petals on germination and primary growth of cotton (gossypium hirsutum L.). Acta Hort., 739: 87-91

[15.] FAO, 2008. www.faostat.org

[16.] Fernandez, J.A., 2004. Biology, biotechnology and biomedicine of saffron. Recent Res. Devel. Plant. Sci., 2: 127-159.

[17.] Ghaffari, S.M., 1986. Cytogenetic studies of cultivated Crocus sativus (Iridaceae). Plant Syst. Evol., 153: 199-204.

[18.] Ghahreman, A., 1977. Flora of Iran. Published by Research Institute of Forests and Ranglelands (RIFR), Tehran, 16: 1944.

[19.] Grilli Caiola, M., 2004. Saffron reproductive biology. Acta Hort., 650: 25-37.

[20.] Grilli Caiola, M. and A. Canini, 2010. Looking for saffron's (Crocus sativus L.) parents. Func. Plant Sci. Biotech., 4: 1-14.

[21.] Hassan Beygy, S.R., D. Ghanbarian, M.H. Kianmehr and M. Farahmand, 2010. Some physical properties of saffron crocus corm. Cercetari Agronomice in Moldova, 1: 17-29.

[22.] Hill, T., 2004. The Contemporary Encyclopedia of Herbs and Spices: Seasonings for the Global Kitchen, Wiley, 272 p.

[23.] Homes, J., M. Legros and M. Jaziri, 1987. In vitro multiplication of C. sativus L. Acta Hort., 212: 675-676.

[24.] Hosseinzadeh, H., G.H. Karimi and M. Niapoor, 2004. Antidepressant effects of Crocus sativus stigma extracts and its constituents, crocin and safranal, in mice. Acta Hort., 650: 435-445.

[25.] Hosseinzadeh, H., and V. Khosravan, 2002. Anticonvulsant effects of aqueous and ethanolic extracts of Crocus sativus L. stigmas in mice. Arch. Irn. Med., 5: 44-47.

[26.] Hosseinzadeh, H., and H.M. Younesi, 2002. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol., 2: 1-8.

[27.] Kafi, M., M.H. Rashed, A. Koocheki and A. Mollafilabi, 2002. Saffron: Production Technology and Processing. Center of Excellence for Agronomy (Special Crops). Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.

[28.] Karamian, R., and H. Ebrahimzadeh, 2001. Plantlet regeneration from protoplast-derived embryogenic calli of Crocus cancellatus. Plant Cell Tissue Organ Cult., 65: 115-121.

[29.] Karimi, G., H. Hosseinzadeh and P. Khaleghpanah, 2001. Study of antidepressant effect of aqueous and ethanolic of Crocus sativus in mice. Iranian J. Basic. Med. Sci., 4: 11-15.

[30.] Lozano, P., D. Delgado, M. Rubio and J.L. Iborra, 2000. A non destructive method to determine the safranol content of saffron (Crocus sativus L.) by supercritical carbondioxide extraction combined with HPLC and gas chromatography. J. Biochem. Biophys. Methods, 43: 367-378.

[31.] Mathew, B., 1999. Botany, taxonomy and cytology of C. sativus L. and its Allies. In Saffron Crocus sativus L., Eds., Negbi, M. Hardwood Academic Publishers, pp: 19-30.

[32.] Mathew, B., 1982. The Crocuses. B.T. Batsford, London.

[33.] Mathew, B., 1977. Crocus sativus and its Allies (Iridaceae). Plant Syst. Evol., 128: 89-103.

[34.] Milyaeva, E.L., N.S. Azizbekova, E.N. Komarova and D.D. Akhundova, 1995. In vitro of regenerant corms of Saffron (Crocus sativus L.). R. J. PlantPhysiol., 42: 112-119.

[35.] Nehvi, F.A., S.A. Wani, S.A. Dar, M.I. Makhdoomi, B.A. Allie and Z.A. Mir, 2007. New emerging trends on production technology of saffron, Proc. II nd IS on Saffron Bio. and Techno. Eds., A. Koocheki et. al. Acta Hort., 739: 375-381.

[36.] Ochiai, T., S. Ohno, S. Soeda, H. Tanaka, Y. Shoyama and H. Shimeno, 2004. Crocin prevents the death of rat pheochromyctoma (PC-12) cells by its antioxidant effects stronger than those of a-tocopherol. Neurosci. Lett., 362: 61-64.

[37.] Piqueras, A., B.H. Han, J. Escribano, C. Rubio, E. Hellin and J.A. Fernandez, 1999. Development of cormogenic nodules and microcorms by tissue culture, a new tool for the multiplication and genetic improvement of saffron. Agronomie, 19: 603-610.

[38.] Plessner, O., M. Negbi, M. Ziv and D. Basker, 1989. Effects of temperature on the flowering of the saffron crocus (Crocus sativus L.): Induction of hysteranthy. Israel J. Bot., 38: 1-7.

[39.] Premkumar, K., S.K. Abraham, S.T. Santhiya and A. Ramesh, 2003. Protective effects of saffron (Crocus sativus L.) ongenotoxins-induced oxidative stress in Swiss albino mice. Phytother. Res., 17: 614-617.

[40.] Premkumar, K., S. Kavitha, S.T. Santhiya, A.R. Ramesh and J. Suwanteerangkul, 2004. Interactive effects of saffron with garlic and curcumin against cyclophosphamide induced genotoxicity in mice. Asia Pac. J. Clin. Nutr., 13: 292-294.

[41.] Rio's, J.L., M.C. Recio, R.M. Giner and S. Manez, 1996. An update review of saffron and its active constituents. Phytother. Res., 10: 189193.

[42.] Sharafzadeh, SH. and M. Khosh khui, 2004. Effect of precooling and growth regulators on micropropagation of estahban saffron. Iranian J. Hort. Sci. Tech., 5: 129-136.

[43.] Tarantilis, P.A., G. Tsoupras and M. Polissiou, 1995. Determination of saffron (Crocus sativus L.) components in crude plant extract using HPLC UV Visible photodiodearray detectionmass spectrometry. J. Chromatography, 699: 107-118.

[44.] Winterhalter, P. and M. Straubinger, 2000. Saffron-renewed interest in an ancient spice. Food Rev. Int., 16: 39-59.

[45.] Zhang, Y., Y. Shoyama, M. Sugiura and H. Saito, 1994. Effects of Crocus sativus L. on the ethanol-induced impairment of passive avoidance performances in mice. Biol. Pharm. Bull., 17: 217-221.

Shahram Sharafzadeh

Islamic Azad University, Firoozabad Branch, Firoozabad, Iran

Corresponding Author

Shahram Sharafzadeh, Department of Agriculture, Islamic Azad University, Firoozabad Branch, Firoozabad, Iran.

E-Mail: s.sharafzadeh@iauf.ac.ir or shahramsharafzadeh@hotmail.com; Tel: +98-9177158317.
COPYRIGHT 2011 American-Eurasian Network for Scientific Information
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2011 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Original Article
Author:Sharafzadeh, Shahram
Publication:Advances in Environmental Biology
Article Type:Report
Geographic Code:7IRAN
Date:Jun 1, 2011
Words:3358
Previous Article:Grain development of wheat in relation to levels of catalase, peroxidase and ascorbic acid peroxidase.
Next Article:Effects of erythropoietin on cell death following of renal ischemic reperfusion in rats.
Topics:

Terms of use | Privacy policy | Copyright © 2021 Farlex, Inc. | Feedback | For webmasters