SPCImage NG TCSPC-FLIM data analysis software.

SPCImage NG is a new generation of Becker and Hickl's time-correlated single photon counting (TCSPC)-fluorescence lifetime (FLIM) data analysis software. It combines timedomain and frequency-domain analysis, uses a maximum-likelihood algorithm to calculate the parameters of the decay functions in the individual pixels, and accelerates the analysis procedure by GPU processing.

1D and 2D parameter histograms are available to display the distribution of the decay parameters over the pixels of the image, or over selectable ROIs. Image segmentation can be performed via the phasor plot, and pixels with similar phasor signature be combined for high-accuracy time-domain analysis. SPCImage NG provides decay models with one, two, or three exponential components, incompletedecay models, and shifted-component models. Another important feature is advanced IRF modelling, making it unnecessary to record IRFs for time-domain analysis. www.becker-hickl.com