Russell's viper venom test.
A Lupus anticoagulants are antibodies that interfere with the phospholipid-dependent phases of the coagulation process. The dilute Russell's viper venom test is a phospholipid-limiting assay that helps to identify the lupus anticoagulant, which frequently poses diagnostic problems for the laboratory as well a therapeutic difficulties for the clinician. Brandt's article should help to sort out this confusing condition. 
In the coagulation laboratory at Duke Medical Center, they use a product marketed by American Diagnostica (Greenwich, CT). In fact, the laboratory did a thorough evaluation of this product published several years ago. Twenty-six healthy donors had negative results. A group of 42 patients with LAG were also studied. The newer formulation of the test indicated that it was more sensitive to LAG than older formulations.  The facility has continued to use this product, but laboratorians comment that another company, Gradipore (North Ryde, Australia), makes a similar product they feel is comparable.
John A. Koepke, MD
(1.) Brandt JT, Triplett DA, Alving B, et al. Criteria for the diagnosis of lupus anticoagulants: An update. Thromb Haemost. 1995;74:1185-1190.
(2.) Joyner KA, Ortel TL. A sensitive dRVVT reagent system for the detection of lupus anticoagulants. Clin Appl Thrombosis/Haemostasis. 1995; 1:73-75.
|Printer friendly Cite/link Email Feedback|
|Publication:||Medical Laboratory Observer|
|Article Type:||Brief Article|
|Date:||Jul 1, 2000|
|Previous Article:||Duplicate orders.|
|Next Article:||Quick-freezing specimens.|