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Ruminal digestion and chemical composition of new genotypes of buffelgrass (Cenchrus ciliaris L.) under irrigation and fertilization.

SUMMARY

The study was conducted with the aim of evaluating and comparing the total dry matter production (TDMP), chemical composition and effective degradability of dry matter (EDDM), crude protein (EDCP) and neutral detergent fiber (EDNDF) of the Nueces hybrid and five new genotypes (PI 1, PI 2, PI 3, PI 4, PI 5) of buffelgrass. Grasses were irrigated and fertilized with 100kgx[ha.sup.-1] of urea-N, and hand harvested on June 5, 2001 at Nuevo Leon, Mexico. All grasses were established on a completely randomized design with three replicates. The TDMP was significantly different among genotypes. The crude protein content and cell wall components (cellulose, hemicellulose, and lignin) were significantly different among grasses. Moreover, EDDM, EDCP, and EDNDF were significantly different among genotypes. The P, Na, Cu and Zn contents, in all grasses, were insufficient to meet growth requirements of beef cattle. Data of dry matter production and nutritional dynamics suggest that the new genotypes PI 3 and PI 4 could be considered good sources of nutrients for grazing ruminants in Northeastern Mexico.

KEYWORDS / Buffelgrass / Chemical Composition / Nutrient Digestibility / Ruminal Digestion /

RESUMEN

El estudio se llevo a cabo con el objetivo de evaluar y comparar el centenide nutritivo y degradabilidad efectiva de la materia seca (DEMS), proteina cruda (DEPC) y pared celular (DEFDN) del hibrido Nueces y cinco nuevas lineas (PI 1, PI 2, PI 3, PI 4, PI 5) de pasto buffel en el nereste de Mexico. Tedes les pastos se establecieron usando un diseno completamente al azar con tres repeticiones. Fueron regados y fertilizades con 100kg x [ha.sup.-1] de urea-N y cosechades manualmente el 5 de junio de 2000 en Nuevo Leon, Mexico. La produccion de materia seca fue significativamente diferente entre los pastos.

El centenido de proteina cruda, pared celular y sus componentes (celulosa, hemicelulosa y lignina) fueren significativamente diferentes entre los pastes evaluades. Asimisme, DEMS, DEPC y DEFDN fueron significativamente diferentes entre pastes. Les contenidos de P, Na, Cu y Zn no fueren suficientes para satisfacer los requerimientos del ganado de carne en crecimiento. Datos de produccion de materia seca y dinamica nutricional sugieren que los nuevos genotipos PI 3 y PI 4 pueden ser considerados como buenas fuentes de nutrientes para el ganado en pastoreo en regiones del noreste de Mexico.

RESUMEN

O estudo se realizou com o objetive de avaliar e comparar o conteude nutritivo e degradabilidade efetiva da materia seca (DEMS), proteina crua (DEPC) e parede celular (DEFDN) de hibrido Nozes e cinco nevas linhas (PI 1, PI 2, PI 3, PI 4, PI 5) de capim buffel no noroeste de Mexico. Todos es capins se estabeleceram usando um desenho completamente aleatorio com tres repeticoes. Feram regades e fertilizades com 100 kg.ha-1 de ureia-N e recelhidos manualmente em 5 de junho de 2000 em Nueve Leon, Mexico. A preducao de materia seca foi significativamente diferente entre os capins. O conteude de proteina crua, parede celular e seus cempenentes (celulesa, hemicelulese e lignina) foram significativamente diferentes entre os capins avaliades. Assim mesme, DEMS, DEPC e DEFDN foram significativamente diferentes entre capins. Os conteudos de P, Na, Cu e Zn nao foram suficientes para satisfazer os requerimentes de gado de carne em crescimente. Dados de preducao de materia seca e dinamica nutricional sugerem que os novos genotipes PI 3 e PI 4 podem ser considerades como boas fontes de nutrientes para o gado em pastereio em regioes de noroeste de Mexico.

Introduction

Common buffelgrass (Cenchrus ciliaris L.) is widely disseminated in semiarid regions of Texas and Northeastern Mexico; hewever, seasonality of rainfall and low temperatures are the major influences on its nutritional quality (Ramirez et al., 2003b). Previous studies carried out by Garcia-Dessemmes et al. (2003a, b) have reported that five new genotypes of buffelgrass, produced without irrigation, were less sensitive to environmental factors, yielding more dry matter and CP content than common buffelgrass and the Nueces hybrid.

Among forages, crude protein (CP) levels are well correlated with many desirable plant components like digestibility, vitamins, Ca and P. However, all these decline to deficient levels at about the same time, and CP serves as a reliable measure of overall nutritional quality (Ganskoop and Bohnert, 2001). Furhtermere, CP values of a number of grasses showed lower mean values (11.5%) than legumes (17.0%) collected in different regions of the world (Minsen, 1990), whereas tropical grasses have lower CP than temperate grasses (means= 10.0 and 12.9%, respectively; Minsen, 1992). Several factors affect CP in grasses. One of the most important ones is the [N.sub.2] content in soils, and it has been shown that N fertilization in temperate grasses increases CP (Whitehead, 2000). However, N fertilization did not affect dry matter or cell wall digestibility of beef cattle grazing temperate grasses (Puoli et al., 1991).

Grazing ruminants are dependent on an adequate supply of minerals for optimal rumen microbial activity. Under certain circumstances, grasses can provide adequate amounts of essential minerals for ruminants. However, grasses often have deficiencies in one or mere minerals and, thus, supplementation is required for optimal animal performance and health (Underweed and Suttle, 1999). This study was conducted with the aim to evaluate and compare the nutritional quality of new buffelgrass genotypes under irrigation and fertilization with urea-N.

Material and Methods

This study was carried eut at the Experimental Station "General Teran", Instituto Nacional de Investigaciones Ferestales, Agricolas y Pecuarias (INIFAP) and the Universidad Autonema de Nuevo Leon (UANL). General Teran, N.L., Mexico, is at 25[degrees]18'N and 99[degrees]35'W, at an altitude of 332masl. The climate is typically semitropical and semiarid, with a warm summer. The main and most common type of vegetation is known as the Tamaulipan Thorn scrub or subtropical Thorn scrub woodlands. The dominant soils are deep, dark-gray, lime-clay Vertisoles, which are the result of alluvial processes. These soils are characterized by high calcium carbonate (pH= 7.5-8.5) and relatively low organic matter content. Annual mean temperature is 22.4[degrees]C and the annual rainfall average is 784mm (INIFAP, 1991).

Five strains of buffelgrass PI-307622 (PI 1), PI-409252 (PI 2), PI-409375 (PI 3), PI-409443 (PI 4) and PI-409460 (PI 5), as well as the hybrid buffelgrass Nueces, which was considered as a reference grass with high nutritional quality, were established in an experiment under a completely randomized design with three replicates. The experimental plots consisted of rows 5m long with 0.8m between rows. In order to achieve a uniform grass growth, all grasses were cut prior to the experiment, on March 1, 2001. The grasses were irrigated four times during the experiment, en March 15, April 15, May 1, and May 15 to avoid any water stress in the plants. On March 15 the experimental plot was fertilized with the equivalent of 100kg x [ha.sup.-1] of urea-N. Plants reached full blossom by June 5, and were then harvested by hand to 0.15m above ground. Partial dry matter was determined in an even at 55[degrees]C for 72h. Then samples were ground in a Wiley mill (1mm screen) and stored in plastic containers for further analyses.

Samples were analyzed for dry matter (DM), organic matter (OM), crude protein (CP), (AOAC, 1997), neutral detergent fiber (NDF) and acid detergent fiber (ADF), Van Soest et al. (1991) and acid detergent lignin (ADL), (AOAC, 1997). Hemicellulose (NDF-ADF) and cellulese (ADF-ADL) were estimated by difference. Estimation of insoluble [N.sub.2] in NDF (INNDF) and insoluble [N.sub.2] in acid detergent fiber (INADF), which corresponds to the nondegraded [N.sub.2] was performed by procedures of Van Soest et al. (1991) and the slewly degraded [N.sub.2] associated to the cell wall components was calculated as INNDF minus INADF (Krishnamoorthy et al., 1982).

Mineral content was estimated by incinerating the samples in a muffle even at 550[degrees]C during 4h. Ashes were digested in a solution containing HC1 and HN[O.sub.3], using the wet digestion technique (Diaz-Remeau and Hunter, 1978). Concentrations of Ca, Na, K, Mg, Cu, Fe, Zn, Mn and Mo were estimated using an atomic absorption spectrophotometer with an air/acetylene flame. The P content was determined in a colorimeter (AOAC, 1997).

The rate and extent of DM, CP and NDF digestibility in grasses were measured using the nylon bag technique. Four rumen fistulated PelibueyxRambouillet sheep (45.2 [+ or -] 2.3kg, BW) were used to incubate bags (5xl0cm, 53[micro]m pore size) that contained ground (4g) samples of each grass and suspended in the ventral part of the rumen of each sheep. With the purpose to sustain adequate rumen microbial activity throughout the experiment, sheep were fed alfalfa hay ad libitum. For each grass, bags were incubated at 4, 8, 12, 24, 36, and 48h. Upon removal from the rumen; bags were washed in cold running water. Zero time disappearance was obtained by washing nonincubated bags (0h) washed in a similar fashion. All bags were dried at 60[degrees]C in an even during 48h. Weight less of DM, CP and NDF was recorded. Disappearance of DM, CP, or NDF for each incubation time was calculated as

Disappearance, % = (Initial weight] - Final weight/Initial weight) x 100

Disappearances of DM, CP, or NDF for each incubation time were used to estimate the digestion characteristics of DM, CP and NDF using the equation (Orskov and McDonald, 1979)

p = a+b (1-[e.sup.ct])

where p: disappearance rate at time t, a: an intercept representing the portion of DM or CP or NDF soluble at the beginning of incubation (t=0), b: portion that is slowly degraded in the rumen, c: constant rate of disappearance of fraction b, and t: time of incubation.

The nonlinear parameters a, b, and c as well as the effective degradability of DM (EDDM) or CP (EDCP) or NDF (EDNDF) = (a+b)c/(c+k)([e-.sup.(ct)LT]), were calculated using the Neway computer program (McDonald, 1981). Here, k: estimated rate of outflow from the rumen, and LT: lag time. The EDDM, EDCP and EDNDF values were estimated assuming a rumen outflow rate of 2.0%/h. Digestible dry matter was calculated as TDMxEDDM and DCP was calculated as TDMxEDCP. Data of chemical composition, a, b, c, EDDM, EDCP, EDNDF, DDM and DCP were statistically analyzed using a one-way analysis of variance. Means were separated (P<0.05) using the least significant difference technique. (Snedecer and Cechran, 1980).

Results and Discussion

Total dry matter production (TDMP) was significantly different among genotypes. PI 3 yielded mere than ether grasses (Table I), including Nueces, which has been recognized as a high producer of dry matter. As it would be expected, irrigation and fertilization led to a higher TDMP production than that of the same grasses (mean of 4.7[ton x [ha.sup.-1] in two cuts) growing in the same area, but collected at different dates and without irrigation or fertilization (Garcia-Dessommes et al., 2003a, b).

The CP content was also significantly different among genotypes. PI 5 had the highest and PI 1 the lowest CP values. The mean CP for all genotypes of 8.7% is ~2% above the minimum (7%) required to sustain rumen functionality in beef cattle (NRC, 1996). Related studies carried out in summer and autumn 1999 (Garcia-Dessommes et al., 2003a, b) using the same genotypes growing in the area but without irrigation or fertilization, had lower CP contents (average of 7.1%).

Therefore, N fertilizafion in this study increased the CP content in all grasses. This effect has already been reported by Minsen (1992) and by Whitehead (2000), who found that the CP content of grasses depends en soil N availability and that N fertilization increases it.

The INNDF, INADF, and INNDF-INADF fractions were significantly different among grasses. The non-available N for ruminants estimated by the INADF fraction was higher (mean value of all grasses= 24.8%) than previous reports (3-15%; Van Soest, 1994) in temperate grasses. Buffelgrass PI 3 had the largest fully digestible portion of the NDF (100-INNDF= 31.3%) and PI 5 the lowest (26.2%). This fraction comes from true proteins, which in grasses constitute the enzymatic machinery that is rapidly degraded in the rumen and transformed in microbial protein (Van Seest, 1994).

The cell wall (NDF) content and its components (ADF, cellulose, and hemicellulose) were significantly different among grasses (Table I). PI 5 resulted with the highest NDF and PI 3 with the lowest. The irrigation and fertilization of buffelgrass plants with urea-N, evaluated in this study had about the same NDF content than that the same plants, but collected in summer and autumn 1999 and autumn 2000 (Garcia et al., 2003a, b; Morales-Rodriguez, 2003) and without irrigation and fertilization. Even though cellulose and hemicellulose are degraded by the same rumen bacteria (Ruminococcus flavefaciens, R. albus and Fibrobacter succinegens), cellulose duplicated hemicellulese content in all grasses (Table I). The hybrid Nueces resulted with the lowest ADL and PI 5 with the highest. Irrigation and fertilization with ureaN lowered ADL (mean = 4.3 [+ or -] 0.4%) compared with 5.5 and 7.0% obtained in previous studies carried out with the same grasses, but on different collection dates (Garcia et al., 2003a, b; Morales-Rodriguez, 2003).

The fraction a (lost during the bag washing process) of the DM, CP, and NDF was significantly different among grasses (Table II). The fraction b (slowly degraded in the rumen of sheep) of the DM was net different (P>0.05); however, significant differences were obtained for CP and NDF. Degradation rate (c, % x [h.sup.-1]) was significantly different only in DM and CP. The EDDM, EDNDF, and EDCP were significantly different among all grasses (Table II). The EDCP overall mean was estimated to be 47.3% and the potential available CP (100INADF) was 75.2%. The difference between these two values (27.9%) corresponds to the value obtained by difference (INNDF-INADF), which is slowly available to rumen microbes, but could be fully digested in the abomasums (Van Soest, 1994). Digestion carried out in abomasums and the small intestine is also a vital process for the ruminant (Merchen and Bourquin, 1995).

In this study, all grasses resulted with lower EDCP than the same grasses collected at different dates and without irrigation or fertilization (Gar, cia-Dessommes et al., 2003a, b). Moreover, in a study conducted by Puoli et al. (1991) it was found that the addition of 75kg x [ha.sup.-1] of urea-N increased dry matter intake of beef cattle grazing Bermuda grass; however, they did not obtain increments in digestibility of CP and cell wall. In this study, EDNDF values resulted lower. than the same genotypes collected in different dates without irrigation or fertilization (Garcia-Dessommes et al. 2003a, b). It is possible that lignification of the cell wall was related to low degradability of nutrients contained in the genotypes evaluated in this study. This effect was also found by Akin and Chesson (1990), who found that high levels of ADL in grass resulted in lower ruminal DM digestion and volatile fatty acid production.

With the exception of Na and Zn all minerals studied were different (P < 0.05) among grasses (Table III). Ca, K, Mg, Fe and Mn contents were higher in irrigated and fertilized grasses than the same grasses without irrigation or fertilization, and collected at different dates (Garcia-Dessommes et al., 2003a, b). In this study, all grasses had sufficient Ca to meet growing beef cattle requirements (4.5g x [kg.sup.-1] DM; McDowell, 2003), but the amount of P in all cases was insufficient for growing beef cattle needs (3.0g x [kg.sup.-1] DM; McDowell, 2003). Garcia-Dessommes et al. (2003a, b) and Morales-Rodriguez (2003) also found low P content in new genotypes of buffelgrass growing in the same area. Thus, cattle grazing these grasses must be supplemented with P. Growing beef cattle needs about 1.0g x [kg.sup.-1] DM of Mg (McDowell, 2003). In this study, all genotypes had sufficient Mg content to satisfy the requirements of this nutrient, as was that of K, which met the requirements for growing beef cattle (6.0g x [kg.sup.t] DM; McDowell, 2003). Growing beef cattle requires 0.6g x [kg.sup.1] DM of Na in their diets (McDowell, 2003); in this study all grasses had insufficient Na and can be considered as Na non-accumulators because they contain less than 2g x [kg.sup.1] DM of Na (Youssef, 1988). Furthermore, high K content in the evaluated grasses could reduce Na absorption of cattle feeding on them, because it has been reported that an elevated dietary K may decrease ruminal concentration and absorption of Na in steers (Spears, 1994). However, Na deficiencies can be alleviated by supplementing common salt.

Cattle consuming the evaluated grasses must be supplemented with Cu, as the latter contained insufficient amounts to meet growing beef cattle requirements (10mg x [kg-.sup.-1]; McDowell, 2003). Low Cu concentrations were also reported in cultivated grasses growing in semiarid regions of Northeastern Mexico (Ramirez et al., 2002a, b, 2005). A low Cu in the evaluated grasses may be caused by the high pH (7.58.5) in the soils (Spears, 1994) in these regions. Growing beef cattle requires about 50g x [kg.sup.-1] of Fe in the DM of their diets (McDowell, 2003). In this study, all grasses had adequate Fe amounts to meet such requirements. Similar findings were reported in previous studies (Ramirez et al., 2002a, b, 2003a, b, 2005) carried out in the same region. Iron deficiency seldom occurs in grazing ruminants due to generally adequate forage concentrations and contaminants of plants by soil (McDowell, 2003). All the grasses studied had sufficient amounts of Mn to meet requirements of growing beef cattle (20g x [kg.sup.-1] of DM; McDowell, 2003). Although Mn deficiency for ruminants under grazing conditions has been reported in USA and other countries (McDowell, 1985), with effects on skeletal development and reproductive performance, doubt has been expressed whether this deficiency arises under field conditions in Mexico. In the report by McDowell (1985), contrary to our findings the Zn content was significantly different among all grasses within seasons; in the present study, Zn content was marginally deficient to meet growing beef cattle requirements (30g x [kg.sup.-1] DM; McDowell, 2003). Similar findings were reported by Garcia-Dessommes et al. (2003a, b), who evaluated the Zn content of the same grasses collected at different dates, but without irrigation or fertilization. High levels of Ca, found in this study, may increase the dietary Zn requirements (Underwood and Suttle, 1999) and therefore, supplemental Zn is required.

Conclusions

The grasses evaluated in the present study yielded more dry matter than they did when collected without irrigation or fertilization and at different dates. The same pattern was observed in CP, EDDM, Ca, K, Mg, Na, Fe and Mn. However, NDF, ADF, cellulose, hemicellulose, INNDF, INADF, P, Cu and Zn remained the same. Lower values were obtained in lignin, EDCP and EDNDF. Concentrations of P, Na, Cu, and Zn in ali grasses were insufficient to meet growing beef cattle requirements. The genotype PI 4 resulted in higher dry matter production, CP and EDCP. In general, all genotypes had nutritional qualities comparable to the hybrid buffelgrass Nueces, which in this study was used as a reference grass with good nutritional quality. Cattle grazing these grasses must be supplemented with E Na, Cu, and Zn.

Received: 0510412006. Modified: 03/29/2007. Accepted: 04/0412007.

REFERENCES

Akin DE, Chesson A (1990) Lignification as the major factor limiting forage feeding value especially in warm conditions. In Proc. XVI Int. Grassland Cong., Vol. III. Association Francaise pour la Production Fourragere. Versailles, France. pp. 1753-1760.

AOAC (1997) Official Methods of Analysis. 17th ed. Association of Official Agricultural Chemists. Washington DC, USA.

Diaz-Romeau RA, Hunter P (1978) Metodologia para el muestreo de suelos y tejidos de investigacion en invernadero. Mimeo. CATIE. Turrialba, Costa Rica. 26 pp.

Ganskoop D, Bohnert R (2001) Nutritional dynamics of 7 northern Great Basin grasses. J. Range Manag. 54: 640-647.

Garcia-Dessommes G J, Ramirez-Lozano RG, Foroughbakhch R., Morales-Rodriguez R, Garcia-Diaz G (2003a) Ruminai digestion and chemical composition of new genotypes of buffelgrass (Cenchrus ciliaris L.). Interciencia 28: 220-224.

Garcia-Dessommes GJ, Ramirez-Lozano RG, Foroughbakhch R, Morales-Rodriguez R, Garcia-Diaz G (2003b) Valor nutricional y digestion ruminal de cinco lineas apomiticas del pasto buffelgrass (Cenchrus ciliaris L.). Tecnica Pecuaria en Mexico 41: 209-218.

INFAP (1991) Memorias Segunda Reunion Cientifica Forestal y Agropecuaria del Estado de Nuevo Leon. Centro de Investigacion Regional del Noreste, Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias. Mexico. 48 pp.

Krishnamoorthy UC, Muscato TV, Sniffen CJ, Van Soest PJ (1982) Nitrogen fractions in selected feedstuffs. J. Dairy Sci. 65: 217-220.

McDonald I (1981) A revised model for estimation of protein degradability in the rumen. J. Agr. Sci. 96: 251-252.

McDowell LR (1985) Nutrition of Grazing Ruminants in Warm Climates. Academic Press. New York, USA. 56 pp.

McDowell LR (2003) Minerals in Animal and Human Nutrition. 2nd ed. Elsevier. Netherland. pp. 26-86.

Merchen NR, Bourquin LD (1995) Processes of digestion and factors influencing digestion of forage based diets by ruminants. In Fahey GC Jr. (Ed.) Forage Quality, Evaluation, and Utilization. University of Nebraska. Lincoln, NE, USA. pp. 564-612.

Minson DJ (1990) Forage in Ruminant Nutrition. Academic Press. San Diego, CA, USA. 188 pp.

Minson DJ (1992) Composicion quimica y valor nutritivo de gramineas tropicales. In Skerman PJ, Riveros F (Eds.) Gramineas Tropicales. Coleccion Produccion y Proteccion Vegetal. FAO. Roma, Italia. 166 pp.

Morales-Rodriguez R (2003) Produccion de materia seca y digestibilidad in situ del forraje de 86 genotipos del pasto buffel (Cenchrus ciliaris). Tesis. Universidad Autonoma de Nuevo Leon. San Nicolis de los Garza, NL, Mexico. 56 pp.

NRC (1996) Nutrient Requirements of Beef Cattle. 7th ed. National Academy Press. Washington, DC, USA. 76 pp.

Orskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. J. Agr. Sci. 92: 499-503.

Puoli JR, Jung GA, Reid RL (1991) Effects of nitrogen and sulfur on digestion and nutritive quality of warm-season grass hays for cattle and sheep. J. Anim. Sci. 69: 843-852.

Ramirez RG, Foroughbackhch R, Gonzalez-Rodriguez H, Garcia-Castillo CG, Alba-Avila J, Hauad LA (2002a) Variacion estacional del contenido mineral en la planta completa, hojas y tallos del zacate buffel comun. (Cenchrus ciliaris L.). Livestock Res. Rural Devel. 14: 22-31.

Ramirez-Lozano RG, Gonzalez-Rodriguez H, Garcia-Dessommes G (2002b) Chemical composition and rumen digestion of forage from kleingrass (Panicum coloratum). Interciencia 27:705-709.

Ramirez RG, Gonzalez-Rodriguez H, Garcia-Dessommes G (2003a) Nutrient digestion of common bermudagrass (Cynodon dactylon L.) Pers. growing in northeastern Mexico. J. Appl. Anim. Res. 23: 93-102.

Ramirez RG, Garcia-Dessommes G, Gonzalez Rodriguez H (2003b) Valor nutritivo y digestion ruminal del zacate buffel comun (Cenchrus ciliaris L.). Pastos y Forrajes 26: 149-158.

Ramirez RG, Gonzalez-Rodriguez H, Garcia-Dessommes G, Morales-Rodriguez GR (2005) Seasonal Trends in the Chemical Composition and Digestion of Dichanthium annulatum (Forssk.) Stapf. J. Appl. Anim. Res. 28: 35-40.

Snedecor GW, Cochran WG (1980) Statistical Methods. 7th ed. Iowa State University Press. Iowa USA. pp: 215-233.

Spears JW (1994) Minerals in forages: In Fahey CG Jr (Ed.) Forage Quality, Evaluation and Utilization. National Conference on Forage Quality. University of Nebraska. Lincoln, NE, USA. pp: 281-311.

Underwood EJ, Suttle NF (1999) The Mineral Nutrition of Livestock. 3rd ed. CABI. Wallingford, UK. 600 pp.

Van Soest PJ (1994) Nutritional Ecology of the Ruminant. 2nd ed. Comstock--Cornell University Press Ithaca, NY, USA. pp. 122-139.

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods of dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74: 3583-3597.

Youssef FG (1988) Some factors affecting the mineral profiles of tropical grasses. Outlook Agric. 17: 104-111.

Whitehead DC (2000) Nutrient Elements in Grasslands. Soil-Plant-Animal relationships. CABI. Wallingford, UK. 135 pp.

Guillermo Juan Garcia-Dessommes. Doctor in Feed Sciences, Institute Nacional de Investigacionos Forestales, Agricolas y Pecuarias (INIFAP). Researcher, INIFAP, Mexico. Address: INIFIAP, Apartado Postal 3, General Teran, N. L: CP 67400. Mexico. e-mail: garcia.guillermo@inifap.geb. mx

Roque Gonzalo Ramirez-Lozano. Ph.D. in Ruminant Nutrition. Professor-Researcher, Universidad Autonoma de Nueve Leon (UANL), Mexico. e-mail: reqramir@feb.uanl.mx

Rocio Morales Rodriguez. Doctoral Candidate, UANL, Mexico. Graciela Garcia-Diaz. Doctor in Biotechnology, UANL, Mexico. en Ciencias en Biotecnologia, UANL, Mexico.
TABLE I
DRY MATTER PRODUCTION (TON/HA), CRUDE PROTEIN, CELL WALL COMPONENTS
AND [N.sub.2] ASSOCIATED TO CELL WALL (%) IN THE FORAGE OF THE HYBRID
NUECES AND FIVE NEW GENOTYPES OF BUFFELGRASS (Cenchrus ciliaris L)

                                 Genotype

Concept           Nueces      PI 1      PI 2      PI 3

TDMP              6.0 b      6.9 ab     7.3 ab    9.9 a
Organic matter   90.1 a     89.0 b     90.1 a    88.2 bc
Crude Protein     8.8 c      8.0 c      8.5 d     9.1 b
NDF              72.1 abc   70.7 bcd   69.7 cd   68.7 d
ADF              48.2 c     51.9 a     51.9 a    48.5 bc
Hemicellulose    23.9 a     18.8 bc    17.8 c    20.2 b
Cellulose        39.7 b     43.2 a     40.3 b    39.6 b
ADL               3.3 d      3.9 c      5.4 b     4.1 c
INNDF            44.9 ab    41.6 bc    46.7 a    45.5 a
INADF            30.3 a     22.9 c     24.0 c    27.2 b
INNDF-INADF      14.6 c     18.7 b     22.7 a    18.3 b

                                 Genotype

Concept            PI 4       PI 5     Mean [+ or -] SEM

TDMP              7.4 ab     6.4 b      7.3 [+ or -] 0.5
Organic matter   88.1 c     90.4 a     89.3 [+ or -] 0.3
Crude Protein     8.1 e      9.6 a      8.7 [+ or -] 0.1
NDF              72.7 ab    73.8 a     71.3 [+ or -] 0.5
ADF              49.8 b     49.3 bc    49.9 [+ or -] 0.4
Hemicellulose    22.6 a     23.7 a     21.2 [+ or -] 0.6
Cellulose        40.4 b     36.7 c     40.0 [+ or -] 0.5
ADL               3.4 d      6.0 a      4.3 [+ or -] 0.4
INNDF            40.5 c     36.3 d     42.6 [+ or -] 1.0
INADF            24.5 c     19.9 d     24.8 [+ or -] 0.8
INNDF-INADF      16.0 bc    16.4 bc    17.8 [+ or -] 0.8

TDMP: total dry matter production, NDF: neutral detergent fiber,
ADF: acid detergent fiber, ADL: acid detergent lignin,
INNDF: insoluble nitrogen in NDF as % of the total crude protein,
INADF: insoluble nitrogen in ADF as % of the total crude protein,
INNDF-INADF: slowly degraded N, associated to the cell wall
components, as % of the total crude protein.

Means in a row with different letter superscripts
are different (P<0.05).

TABLE II
DIGESTION CHARACTERISTICS AND EFFECTIVE DEGRADABILITY OF THE DRY
MATTER, CRUDE PROTEIN AND NEUTRAL DETERGENT FIBER IN THE FORAGE
OF THE HYBRID NUECES AND FIVE NEW GENOTYPES OF BUFFELGRASS
(Cenchrus ciliaris L)

                             Genotype

Concept       Nueces      PI 1      PI 2      PI 3

EDDM, %       54.2 a    43.1 cd    42.0 d    51.5 ab
a,%           26.8 a    21.6 c     21.6 c    27.7 a
b, %          39.7 a    32.3 a     30.2 a    37.9 a
c, %/h         6.0 a     5.3 a      5.9 a     4.9 a
DDM, ton/ha   3.2 b      3.0 b     3.1 b      5.0 a

EDCP, %       46.2 b    41.1 bc    44.2 bc   59.3 a
a,%           23.1 c    25.0 b     18.2 e    30.3 a
b, %          34.8 bc   29.5 d     40.0 a    41.0 a
c, %/h         5.2 a     5.8 a      4.8 a     7.0 a
DCP, kg/ha    0.24 b    0.25 b     0.27 b    0.54 a

EDNDF, %      52.4 a    41.7 b     37.2 c    42.8 b
a,%           20.4 a    16.6 b     15.6 b    18.1 ab
b, %          46.1 a    38.4 b     31.4 c    35.7 b
c, %/h         6.4 ab    5.1 b     6.4 ab    5.9 ab

                             Genotype

Concept       PI 4      PI 5       Mean [+ or -] SEM

EDDM, %       48.0 bc   46.0 bcd   47.5 [+ or -] 1.3
a,%           24.3 b    21.7 c     24.0 [+ or -] 0.7
b, %          35.2 a    35.5 a     35.2 [+ or -] 1.4
c, %/h         5.6 a     6.2 a      5.7 [+ or -] 0.2
DDM, ton/ha    3.6 ab    3.0 b      3.5 [+ or -] 0.3

EDCP, %       45.5 bc   43.6 c     47.3 [+ or -] 1.3
a,%           21.4 cd   20.4 a     23.1 [+ or -] 1.0
b, %          36.0 b    32.3 cd    35.6 [+ or -] 1.0
c, %/h         5.1 a     7.2 a      5.8 [+ or -] 0.3
DCP, kg/ha    0.27 b    0.27 b      0.3 [+ or -] 0.03

EDNDF, %      44.1 b    44.2 b     43.7 [+ or -] 1.2
a,%           19.0 ab   19.5 ab    18.2 [+ or -] 0.6
b, %          35.5 b    36.0 b     37.2 [+ or -] 1.2
c, %/h         6.8 a     6.1 ab     6.1 [+ or -] 0.3

EDDM: effective degradability of the dry matter, EDCP: effective
degradability of crude protein, EDNDF: effective degradability
of neutral detergent fiber, calculated using a rumen outflow rate
of 2.0%/h, a: fraction of DM or CP or NDF (%) lost during washing,
b: fraction of DM or CP or NDF (%) slowly degraded in the rumen
of sheep, c: degradation rate of DM or CP or NDF (%/h),
DDM: digestible dry matter (TDMxEDDM), DCP: digestible crude
protein (TDMxCPxEDCP).

Means in a row with different letter superscripts
are different (P<0.05).

TABLE III
MACRO AND TRACE MINERAL CONTENT IN THE FORAGE OF THE HYBRID NUECES
AND FIVE NEW GENOTYPES OF BUFFELGRASS (Cenchrus ciliaris L)

                          Genotype

Mineral    Nueces      PI 1       PI 2       PI 3

Ca *        8.9 a      8.3 a      8.3 ab     8.4 a
P *         1.7 ab     1.3 b      1.5 b      1.6 ab
Na *        1.2 a      1.1 a      1.2 a      1.1 a
K *        30.1 b     34.9 b     29.1 b     46.8 a
Mg *        2.6 a      1.7 b      2.0 b      2.3 ab
Cu **       1.8 b      2.2 b      1.8 b      3.3 a
Fe **     125.7 bc   148.3 bc    94.3 c    215.1 a
Zn **      13.0 a     12.4 a     15.3 a     14.9 a
Mn **      37.7 ab    32.5 b     42.2 ab    49.6 a

                           Genotype

fMineral     PI 4       PI 5      Mean [+ or -] SEM

Ca *        8.1 a      8.0 b      8.7 [+ or -] 0.4
P *         1.5 b      l.1 c      1.6 [+ or -] 0.07
Na *        1.2 a      1.2 a      1.2 [+ or -] 0.1
K *        30.1 b     35.1 b     34.3 [+ or -] 1.9
Mg *        2.2 ab     1.9 b      2.1 [+ or -] 1.0
Cu **       2.4 ab     3.1 ab     2.4 [+ or -] 0.2
Fe **     126.3 bc   151.9 b    143.6 [+ or -] 11.5
Zn **      16.3 a     12.4 a     14.1 [+ or -] 0.8
Mn **      49.7 a     29.5 b     40.2 [+ or -] 2.5

* g x kg and ** mg x kg, dry matter basis.

Means in a row with different letter superscripts
are different (P<0.05).
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Title Annotation:REPORTS/COMUNICACIONES/COMUNICACOES
Author:Garcia-Dessommes, Guillermo Juan; Ramirez-Lozano, Roque Gonzalo; Morales-Rodriguez, Rocio; Garcia-Di
Publication:Interciencia
Date:May 1, 2007
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