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Rootstock influencing the quality and biogenic amines content on Syrah tropical wines.


The Sao Francisco Valley, located at the Northeast of Brazil, is a region with a dry and hot climate, with temperatures that are not sufficient to induce the vine dormancy (lower than 12[degrees]C), however, with the present structure and irrigation management is possible the viticulture and the production of fruits with higher quality.

Syrah (Vitis vinifera L.) is an international red cultivar, considered to be one of the noble black grape varieties due to its ability to produce dark, full-bodied, and age-worthy wines (Condurso et al., 2016) and also demonstrated a good performance on the climatic conditions of the Sao Francisco Valley.

The effect of grape rootstock on grape and wine composition is controversial, mainly for red grapes. Many of these tests provide conflicting information, depending on the cultivar, environmental conditions and experimental area. According to Leao et al. (2009), at the Sao Francisco Valley, the rootstocks that have shown satisfactory performance for table and wine grapes are hybrids such as IAC 313, IAC 572 and IAC 766. The rootstock Paulsen 1103 has shown good characteristics for table grape growing and is being tested for grape production for wine.

The concept of wine quality as being a result of local ecological factors has a great and fundamental acceptance in oenology (Somers et al., 2016). Thus, the particular soil and climate characteristics, the cultivar and rootstock combinations are important to wine characteristics. Understanding the mechanisms that may interfere on the phenotypic variability of a grape cultivar resulting in genotype interactions and rootstock mean a step in the study of mobile signals, expression and genetic control of favorable changes in grapevines (Albacete et al., 2015). Nevertheless, the molecular mechanisms associated with the ability of the combination of the rootstock to influence the metabolism of berries is still little elucidate (Berdeja et al., 2015).

Biogenic amines are natural contaminants formed by decarboxylation of amino acids by microorganisms (Preti & Vinci, 2016). Several amines have been identified in wine, in contents up to 50 mg per liter. The occurrence of biogenic amines in wines is important from a technological and toxicological point of view and can be originated from vinification techniques or even according to grape inherent compounds, climate conditions, etc (Preti & Vinci. 2016). Biogenic amines, when in high concentrations, can cause health risk to sensitive individuals, in addition to having a negative effect on wine taste and aroma (Souza et al., 2005; Gloria & Vieira, 2007).

The aim of this study was to evaluate the influence of the rootstock used on Syrah vines on wine quality through evaluations of color, anthocyanins, total sugars, phenolic compounds, antioxidant activity and biogenic amines content.

Materials and Methods

The grape cultivar assessed was Syrah grafted on two different rootstocks: IAC-313 and Paulsen 1103 (1103-P) at the experimental area of the Bebedouro irrigation project of Embrapa Semiarido, in the municipality of Petrolina, in the state of Pernambuco, Brazil (9[degrees]21'S, 40[degrees]40'W, at approximately 350 m of altitude). Two treatments, according to the rootstock used, performed the experiment.

The plants were installed five years before, grown on a drip-irrigated trellis system, spaced at 3.5x2.0 m. Fifteen kg of grapes were harvested per block in the morning at optimal maturity, based on total soluble sugars and total acidity, and were taken to the Enology Laboratory at Embrapa Semi-Arid and placed in cold chambers at 10[degrees]C for 12 hours, before being processed. The grapes for each block were then hand stripped and placed in an experiment container.

The wines were elaborated according to time of harvest and grape varieties using the traditional method (Peynaud e Bloin, 1997) in 200 L stainless steel vats. Alcoholic and malolactic fermentations were performed at 25 and 18[degrees]C, respectively. The wines were then stabilized and bottled.

On the wines, the total soluble solids were determined in a filtered homogenate using a digital refractometer, model PR100 (Atago, Tokyo, Japan), with automatic temperature compensation to 25[degrees]C. Total sugar determinations, which provide the percentage of glucose, were extracted with 70% of ethanol and determined by the Antrona method (Dische, 1962).

Wine color was measured using a colorimeter, model CR400 (Konica Minolta Business Solutions U.S.A., Ramsey, NY, USA), with an 8 mm light path aperture. The instrument was calibrated with a CR400 standard tile (Y=93.5, x=0.3114, y=0.3190) (Konica Minolta Business Solutions U.S.A., Ramsey, NY, USA). The wine color results were characterized by: lightness (L*), which ranges from black (0) to white (100); and chromaticity (C*), which represents color purity.

Wine pH was determined with a pH meter, model Tec3MP (Tecnal, Piracicaba, SP, Brazil). Analysis of total anthocyanins was performed following the method of differential pH (Giusti & Wrolstad, 2001). A solution with acidified methanol was centrifuged at 2,000 g for 15 min, at 4[degrees]C, for sample preparation. An aliquot of the supernatant was diluted with buffers of pH 1.0 and 4.5, and determinations were taken at wavelengths of 510 and 700 nm on a Varian Cary 50 probe spectrophotometer (Varian Medical Systems, Belrose, Australia). The total content of anthocyanins was calculated as cyanidin3glucoside. The total acidity was expressed in tartaric acid (%) according to the titulation of the wine until it reached the pH of 8.2, when all the acid were neutralized by the NaOH.

Phenolic content was determined according to the methodology of Singleton & Rossi Junior (1965) using the FolinCiocalteu reagent. The extraction procedure involved steps of centrifugation, filtration, and rest in order to obtain a better extraction of phenolic compounds. Phenolic content was then determined by a spectrophotometer Varian Cary 50 probe (Varian Medical Systems, Belrose, Australia) at 750 nm, and the absorption values were compared with the standard curve with gallic acid.

The determination of the antioxidant activity was realized by the DPPH method using the Rufino et al. (2007) methodology, based on the DPPH radical scavenging (2,2-Diphenyl-1-picrylhidrazil) by the antioxidants compounds, leading to a decrease of absorbance at 515 nm, with results expressed in free-radical scavenging percentage (%FSR).

Eight biogenic amines were investigated on wines, according to standards of putrescine (PUT) dihydrochloride, spermidine (SPD) trihydrochloride, spermine (SPM) tetrahydrochloride, agmatine (AGM) sulfate, cadaverine (CAD) dihydrochloride, serotonine (SRT) hydrochloride, histamine (HIM) dihydrochloride, tyramine (TYM), tryptamine (TRM) and 2-phenylethylamine (PHM) dihydrochloride purchased from Sigma Chemical Co. (St. Louis, MO, EUA). The mobile phases were filtered in HAWP and HVWP membranes, used respectively for aqueous and organic solvents (47 mm diameter and 0.45 |m pore size, Millipore Corp., Milford, MA, USA). The amines were separated by ion pair reverse phase HPLC and quantified fluorimetrically after post-column derivatization with o-phthalaldehyde. Liquid chromatography was performed on a LC- 10AD system connected to a RF-551 spectrofluorimetric detector at 340 and 445 nm of excitation and emission, respectively, and to a CBM-10AD controller (Shimadzu, Kyoto, Japan) (Souza et al., 2005). A reversed-phase [mu]Bondapak C18 column, 300 x 3.9 mm i.d., 10 [micro]m, was used with a [mu]Bondapak C18 guard-pak insert (Waters, Milford, MA). The mobile phases were: A, solution of 0.2 M sodium acetate and 10 mM 1-octanesulfonic acid sodium salt adjusted to pH 5.0 with acetic acid; and B, acetonitrile. The flow rate was set at 0.8 mL/min and the gradient was: 13 min at 11% B, 19 min at 30% B, 24 min at 11% B, and 45 min at 11% B. The post-column derivatization reagent was delivered at 0.4 mL/min. It consisted of 1.5 mL Brij-35, 1.5 mL mercaptoethanol and 0.2 g ophthalaldehyde dissolved in 500 mL solution of 25 g boric acid and 22 g KOH (pH adjusted to 10.5 with 3% KOH). The column and the post-column reaction apparatus were at room temperature (22 [+ or -] 1[degrees]C). The identification of amines was performed by comparison of retention time of amines in samples to standard solutions and also by addition of the suspected amine to the sample. Amine levels were calculated by direct interpolation in the standard curve.

The study of wines quality and chemical characteristics was carried out in a completely randomized design, with two treatments and four replicates, each. Means were compared by Tukey's test, at 5% of probability, except for biogenic amines, in which the values obtained by HPLC were compared to the standard peak.

Results and Discussion

On Table 1 is possible to observe the quality parameters evaluated on Syrah red wines using different rootstocks. The total soluble solids are an indirect measurement of the sugar content of the sample and the values were statistically different for wines from vines with different rootstocks. On the other hand, the total sugars did not varied statistically according to the wine evaluated, indicating that part of the soluble solids responsible for a significant difference in this parameter are not only related to sugars.

It is possible to observe a higher soluble solids content in wines from Syrah vines with the use of the Paulsen 1103 (1103-P) rootstock. In wines, the sugar content are important for a commercial classification of the wine, as it occurs in Brazil according to legislation stablished by the Brazilian Ministry of Agriculture, for example. If the wine or must have higher sugar content, less sugar will be added at the chaptalization to favoring the fermentation and resulting in a flavor more characteristic of the used grape. Vigorous rootstocks can lead to a higher sugar synthesis in the plant.

For wine color analysis, according to the variables that indicate sample lightness (L*) and chromaticity, responsible for indicating the intensity of the coloration of the product assessed and which is a result of a calculation according to a* and b* color values obtained for wines, no statistical difference was observed (Table 1), although is it possible to observe a tendency of increase on chromaticity values for wines from Syrah vines with the IAC-313 rootstock. The color in wine is a result of the presence of monomeric anthocyanins, explaining the fact that the same wine cited above resulted in anthocyanin content about 9% higher than from wines from vines which used the Paulsen1103 rootstock, although the difference was not considered significant. It can be observed that in Syrah vines grown in the Sao Francisco Valley, Brazil, little interference in berry color is due to the rootstock that was used in the cultivation of vines.

Suriano et al. (2016) Evaluating wines from Italy, affirmed that the genotype/rootstock can interact with chemical characteristics of some grape varieties, being the choice of the rootstock the decision of major importance winemaking technologies results. The same authors observed an influence on the genotypes and rootstocks on the wine anthocyanins content. This difference was not observed in the present study, probably due to the use of the same genotype, with changes only on the rootstock.

A higher acidity (Table 1) can be observed for the wine produced with Syrah grafted on the IAC-313 rootstock. The acid content in wine is a sum of the acids presented in musts with other acids produced during and after the alcoholic fermentation. The acidic compounds play an important role in keeping the wine microbiologically and chemically stable. In addition, they have a large effect on the color, taste and stability of the final product (Vidigal et al., 2016). Some studies also related the role of the rootstock on grape pH (Kodur et al., 2013; Jogaiah et al., 2015). The changes on plant physiology that could interfere on secondary metabolism composition leading to a higher phenolic composition of the wines should be better investigated. Nassur et al. (2014) observed that the grape cultivar can influence the chemical chemical characteristics of grape juices, regardless of the rootstock used.

The moderate consumption of wine, especially red wine, has been associated with health benefits, such as the decreasing the risk of cardiovascular diseases (Lamuela-Raventos & Estruch, 2016). This property is due to the antioxidant effect of some wine constituents. Thus, if it is possible to find some alternatives to increase the antioxidant potential of the beverage, this management would be highly recommended. According to the results on Table 1, the use of the rootstock IAC-313 was responsible for the increase in the antioxidant activity, when evaluated by the DPPH method and also for the higher phenolic content observed in the tropical Syrah red wines.

Some studies evaluated the rootstock influence on vines phenolic compounds and the positive influence of the wine phenolic composition on wine commercial price (Granato et al., 2012), aroma composition (San Juan et al., 2012) and antioxidant properties (Llobodanin et al., 2014) has been reported.

On table 2 is possible to observe the biogenic amines incidence on Syrah tropical red wines according to the rootstock used.

The eight studied amines were putrescine (PUT), cadaverine (CAD), histamine (HIM), tyramine (TYM), serotonin (SRT), agmatine (AGM), spermidine (SPD) and phenylethylamine (PEM). It is possible to observe that when the rootstock IAC-313 was used, only putrescine was detected in the wines. On the other hand, the change on the rootstock for the 1103-Paulsen lead to the observation of three biogenic amines: PUT, AGM and PEM.

Biogenic amines are naturally present in wine and it is very difficult to obtain a wine that does not contain any biogenic amines (Pozo-Bayon et al., 2012)

The occurrence of biogenic amines in wines can originate from winemaking techniques and from grape characteristics. The incidence can be a result of the contact of the must with the grape skin, aging, aminoacid profile, wine contact to the yeats, etc (Preti e Vinci, 2016).

Some people can be intolerant to histamine and normally also intolerant to wine (Konakovsky et al., 2011). Thus, is also important the attention of this consumers regarding to possible reactions of histamine-intolerant, considering foods that can contain amines.

It is important to observe that some oenological practices used to enhance wine flavor and aroma can also increase the concentration of the precursor amino acids of the biogenic amines, such as the ageing of wines and the maceration time (Preti e Vinci, 2016). Also, the type of container employed during malolactic fermentation (stainless steel or oak barrel) seems to affect the biogenic amine content of wines, suggesting that the components of wood, mainly phenolic compounds, may influence the production of biogenic amines by lactic acid bacteria (Jimenez-Moreno et al., 2003).

In the present study it is possible to observe that not only filed practices or conditions and some oenological techniques, but also the rootstock used on Syrah vines can be responsible for the biogenic amines content and the profile of the amines presented in this type of wine produced in a tropical semi-arid condition.

Furthermore, another important parameter for the occurrence of these natural contaminants is pH, which was significantly correlated with putrescine, cadaverine and tyramine. Martin-Alvarez et al., 2006 correlated the formation of BA with high values of pH in wine. In the present study, it is possible to observe that the wines with a lower pH presented only one type of biogenic amines, between the investigated ones.

A pH under 3.3 may cause difficult malolactic fermentation, but a high pH can increase the susceptibility of the wine to microbial spoilage. Some authors have established a critical pH level, above which it is more difficult to control the microorganism population, with the possibility of problems arising due to the production of biogenic amines (Preti et al., 2016).

A higher antioxidant activity and phenolic compounds content were observed for the wine in which only the presence of putrescine was identified. However, a positive correlation with oenological practices and biogenic amines content was observed by Preti et al., (2016), that concluded that according to winemaking techniques, it is possible to observe higher antioxidant properties and the natural formation of amines such as putrescine and cadaverine as a drawback. These two amines have no documented adverse health effects, but can be an important issue for sensitive consumers because they can have a negative synergistic effect with the more toxic histamine and tyramine present in a meal.


The use of the rootstock IAC-313 on Syrah vines at the Sao Francisco Valley semiarid conditions, Brazil, resulted in wines with a lower pH and soluble solid contents and higher antioxidant activity and phenolic contents, with only the presence of putrescine of the studied biogenic amines. The use of the rootstock IAC313 on Syrah vines at the Sao Francisco Valley semi-arid conditions, Brazil, resulted in wines with a lower pH and soluble solid contents and higher antioxidant activity and phenolic contents, with only the presence of putrescine of the studied biogenic amines. When the rootstock 1103 was used, contents of putrescine, agmatine and phenylethylamine were detected, promoting wines with higher pH and soluble solids content.


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Rita de Cassia Mirela Resende Nassur (1*), Giuliano Elias Pereira (2), Maria Beatriz Abreu Gloria (3), Luiz Carlos de Oliveira Lima (4)

(1) Bahia State University, Juazeiro, Ba- Brazil;

(2) Embrapa Semiarid/ Grape and Wine, Petrolina, PE, Brazil

(3) Federal University of Lavras, Lavras, MG, Brazil

(4) Federal University of Minas Gerais, Belo Horizonte, MG, Brazill

(*) Corresponding author, e-mail:

Received: 22 July 2015

Accepted: 13 May 2016

DOI: 10.14295/CS.v8i2.2562
Table 1. Soluble solids (SS), total sugar (TS), lightness (L),
chromaticity (C), antocyanins (AC), pH, acidity (A), phenolics (P),
antioxidant activity (AA) of Brazilian tropical 'Syrah' red wines,
according to the rootstock used.

Wine        SS       TS      L*       C        AC        pH

Syrah-313    9.70 b  1.23 a  11.44a   22.33 a  107.46 a  3.70b
Syrah-1103  10.4 a   1.29 a  10.71a   16.92 a   98.65 a  4.00a

Wine        A        P       AA

Syrah-313   0.69 a   1.63 a  50.19 a
Syrah-1103  0.54 b   1.15 b  42.51 b

Means followed by the same letter in column are not different according
to Tukey's Test at 5% of probability.

Table 2. Biogenic amines ([micro]g [mL-.sup.1]) in Brazilian tropical
'Syrah' wines according to rootstock.

Wine        PUT   CAD  HIM  TYM  SRT  AGM   SPD  PEM

Syrah-313   8.39  0    0    0    0    0     0    0
Syrah-1103  8.04  0    0    0    0    0.15  0    0.22

Putrescine (PUT), cadaverine (CAD), histamine (HIM), tyramine (TYM),
serotonin (SRT), agmatine (AGM), spermidine (SPD) and phenylethylamine
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Author:de Cassia Mirela Resende Nassur, Rita; Pereira, Giuliano Elias; Gloria, Maria Beatriz Abreu; de Oliv
Publication:Comunicata Scientiae
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Geographic Code:3BRAZ
Date:Apr 1, 2017
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