Risk Analysis of Gene Flow from Cultivated, Addictive, Social-Drug Plants to Wild Relatives.
By a considerable margin, most chemical constituents in flowering plants are secondary chemicals--organic compounds produced during metabolism not directly involved in essential biological structures or in normal development or reproduction. Secondary compounds often protect plants against herbivory (Wink, 1988; Maag et al., 2015) or photodamage (Close & McArthur, 2002), act as tools for plant communication (Maag et al., 2015; van Dam & Bouwmeester, 2016) or interspecific competition (Maag et al., 2015), and/or may rarely represent excreted, unwanted metabolic products (Haslam, 1986). Plants that accumulate large amounts of secondary chemicals are often of immense economic significance (Small, 2004; Small, 2006), including culinary herbs and medicinal plants. Accordingly, plants with large concentrations of secondary chemicals are widely cultivated.
Drugs that are consumed for non-medical purposes are often tenned "social drugs" or "recreational drugs." These may be efficacious, useless, or dangerous; legally controlled (sales and/or consumption banned or restricted); employed for spiritual or religious purposes; or consumed alone or in the company of others. As a rule, these drugs are employed to produce a pleasant, satisfying mental state, and, for at least some individuals, the desire for repeated consumption may become addictive. In fact, of all the secondary chemicals produced by plants, among the most employed are those that are addictive. Indeed, most humans consume addictive plant secondary chemicals, often on a daily basis (Van Wyk & Wink, 2017). For instance, caffeine is the world's most universally employed psychoactive chemical, mostly imbibed as coffee (from Cojfea spp.), tea (from Camellia sinensis), cola (from Cola spp.), and mate (from Ilex paraguariensis), and eaten as chocolate (from Theobroma cacao) (Daly et al., 1998; Mitchell et al., 2014). Therefore, the most popular of the addictive, social plant inebriants are very widely grown and consumed, and thus represent an ideal group for examining the potential effects of gene flow from species with very high concentrations of secondary chemicals to their wild relatives that tend to produce less of such chemicals.
Plants grown for their production of addictive chemicals may be capable of transferring genes responsible for their biosynthesis to related species, which in turn could profoundly affect the ecology and evolution of the latter, as well as associated interacting organisms. Like any other crop trait, genes involved in pharmacological chemical pathways can spread via pollen and seed dispersal to populations of related crops, weeds, and wild relatives (Ellstrand, 2003). More so than other crops, gene flow from cultivated, addictive, social-drug plants may have unintended biosafety consequences, given their dramatic effects on vertebrate physiology (e.g., Ankley et al., 2007; Nutt et al., 2010, and later references). Here, we explore the potential ecological risks related to gene flow from cultivated, addictive, social-drug crops, a class of domesticated plants that is becoming increasingly widespread. Our observations are likely applicable to many crops that accumulate very high levels of secondary metabolites (Ellstrand, 2003; FAO. 2004; Snow et al., 2005).
While social-drug plants were historically restricted in distribution, the ranges of many of them are rapidly expanding due to increased cultivation and escape from cultivation through various mechanisms, such as interspecific hybridization and ferality (Plowman, 1979; Plowman, 1980; Hylander & Nemomissa, 2009; Cadena-Gonzalez et al., 2013). Gene flow from crops or uncultivated populations derived from them can alter the pharmacological status of recipient, non-narcotic populations (e.g., Ellstrand et al., 2014). Domesticated plants often hybridize spontaneously with their sexually compatible crop and wild relatives when they come into contact (e.g., Whitney et al., 2010; Khoury et al., 2013; Ellstrand et al., 2014). Moreover, experimental and descriptive studies, using genetic markers, have demonstrated that crop alleles frequently enter and persist in natural populations (e.g., Snow et al., 2010; Campbell et al.. 2016; Jhala et al., 2017).
Ecological consequences of crop-to-wild gene flow may have three consequences: 1) generation of more competitive weeds, 2) altered gene frequencies in wild plant populations, and 3) reduced survival and fecundity of natural herbivores. Here we describe examples of each, especially focusing on examples for which there are potentially negative ecological consequences to explore the possible risks that may arise from gene flow from social drag crops to wild relatives.
Generation of More Competitive Weeds. Gene flow from crops to weeds has commonly resulted in more aggressive or successful weed populations (examples include non-transgenic radish, Bt canola, herbicide resistant canola, herbicide resistant rice, insect resistant rice, Bt sunflower, weedy radish, wild bird's rape, weedy rice, weedy sunflower, among others; Snow et al., 2003; Campbell et al., 2006; Londo et al, 2010; Hovick et al., 2012; Liu et al., 2014; Sagers et al., 2015; Lu et al., 2016). Mechanisms by which the competitiveness of weeds could increase via crop-wild hybridization have been discussed elsewhere (e.g., Ellstrand & Schierenbeck, 2000; Whitney et al., 2006; Campbell & Snow, 2007) but include hybrid vigour (e.g., radish: Snow et al., 2001) and the transfer of alleles that directly influence relative plant competitiveness (such as associated with reduced susceptibility to herbivory (e.g., Snow et al., 2003) or allelopathy (see coffee example)).
Altered Gene Frequencies in Wild Plant Populations. When crop alleles enter the populations of wild relatives, they may persist for long periods (Snow et al., 2010), reducing genetic diversity while increasing genetic homogeneity (Campbell et al., 2016). This may cause the local extirpation (Hegde et al., 2006) or near extinction of the wild relatives (e.g., Oryza rufipogon Griff, subsp.formasana Masam. and S. Suzuki [Kiang et al., 1979]), cotton (Gossypium darwinii G. Watt and G. tomentosum Nutt. [Wendel & Percy, 1990]), and mulberry (Morus rubra L. [Burgess et al., 2005]). The loss of these wild relatives limits the genetic diversity of in situ or ex situ germplasm collections and will thus limit future crop development.
Reduced Survival or Fecundity of Natural Herbivores. When crops produce novel phytochemicals or novel dosages of phytochemicals (e.g., genetically engineered Bt crops, etc.), the growth and reproductive success of herbivore consumers may be inadvertently altered (Snow et al., 2003; Raybould et al., 2007), especially when they consume relatively large quantities of these chemicals--an event that might be more likely to occur in large crop fields (Prasifka et al., 2005). These novel chemical traits can be transferred among cultivare and from crop populations to their wild relatives (Snow et al., 2003; Rong et al., 2005). Nontarget insect consumers of transgenic plants producing Cry proteins from Bacillus thuringiensis have been shown to sometimes experience negative fitness consequences but the consequences depend on the toxicity of the insecticidal proteins and their exposure to the insecticides (Garcia-Alonso et al., 2006, Romeis et al., 2008). For instance, Crylab proteins are unlikely to negatively affect non-lepidopteran herbivores (Glare & O'Callaghan, 2000, US Environmental Protection Agency (USEPA), 2001; Wolfenbarger et al., 2008). However, natural lepidopteran herbivores have been documented to ingest and experience negative impacts of the Bt toxins while consuming Bt genetically engineered plants (e.g., Raybould et al., 2007; Snow et al., 2003). Thus, growing phannacological plants outdoors, in close proximity to herbivore populations, increases their access and may influence organismal health, depending on the dosage consumed, herbivore species, and the activity of the chemical (Hartmann, 1999; Baldwin, 2001; Wynn-Edwards, 2001; Agrawal & Konno. 2009).
The risk of transferring genes that control the expression of social drugs into wild non-drug plants, may also affect domesticated animals that consume altered plants. In stabilized ecological communities, wild animals typically have learned which poisonous plants and fungi to avoid, and are naturally cautious when they encounter unfamiliar plants (Iason & Villalba, 2006). Nevertheless, conceivably, when social drug genes escape into populations of wild relatives or non-cultivated populations via either crop-wild hybridization or ferality, gene transfer may, at least occasionally, imperil local herbivores. More likely, domesticated animals, whose natural instincts have been dulled, are likely to be affected. It is well known that pets and livestock are susceptible to poisoning by eating deadly plants (e.g., Fowler, 1983). Pet animals are unlikely to consume plants outside of urban areas, but livestock, grazing or foraging in wildlands, might well consume plants expressing introgressed traits.
Case Studies: Ten Globally Important Social Drug Crops
The ten social drug crops chosen for analysis include the plant sources of the most popular caffeine beverages (coffee, tea, cola, and mate) and the most commonly misused plant-derived drugs, as identified by Ashihara & Crozier (2001) and Nutt et al. (2010). Some of the widespread plant drugs are obtained from more than one species; we reviewed as many species as were necessary to account for a substantial majority of the drugs harvested. These case studies represent tropical, subtropical, and temperate species belonging to ten taxonomic families (and are presented in alphabetical order). The sample includes crops grown as annuals (Cannabis sativa, Papaver somniferum, Nicotiana tabacum--although Nicotiana is sometimes perennial) and shrubs (Catha edulis, Ephedra spp., Erythroxylum spp, Coffea spp., Camellia sinensis, Cola spp., and Ilex paraguariensis). We used several citation indices (Web of Science, Google Scholar, Agricola) for our literature search (April-October, 2017). For each of the ten groups we reviewed: their taxonomy and geography (with particular regard to establishing the extent to which related uncultivated taxa and populations are potentially interfertile with social drug plants); identification of wild taxa or populations that are in danger of gene loss or even population extinction; pollen and seed vectors (the vehicles which transport genes from cultivated to non- cultivated populations); documented evidence of hybridization and gene transfer; information on the inheritance of the drugs (which affects the ability of the determining genes to develop the drug in targeted populations); information pertinent to the harmfiilness of the drugs in herbivores; and considerations that endanger particular ecosystems.
Coca--Erythroxylum coca Lam., E. novogranatense (Morris) Hieron (Erythroxylaceae) [including four infra-specific taxa: E. coca var. coca, E. coca var. ipadu, E. novogranatense var. novogranatense, E. novogranatense var. truxillense]
Erythroxylum is a genus of about 230 mostly Neotropical species of small trees and shrubs. Cocaine-rich leaves can be obtained from at least 15 species (Bieri et al., 2006) but the primary sources are E. coca var. coca, E. coca var. ipadu, E. novogranatense var. novogranatense, and E. novogranatense var. truxillense (Ganders, 1979). Perhaps as a result of the U.S. "war on drugs" (Briones et al., 2013) and the associated political instability or the plant's long life-cycle, we could not find any publications describing the inheritance of the tropane-ester alkaloid, cocaine (methylbenzoylecgonine). With a heterostylous mating system, these plants tend to outcross (and may be selfincompatible in some taxa), although hand-crosses have demonstrated that selfing is possible in E. coca and E. novagratense var. novagratense (Ganders, 1979). The taxa differ in floral fragrance. The scent of E. coca is foetid, consistent with insect pollination (Ganders, 1979). Fruit may travel long distances since the drupes are dispersed by birds (Ganders, 1979). Finally, E. coca var. coca is planted as seeds whereas E. coca var. i pa du is propagated as cuttings, presumably making the latter more genetically homogenous than the former (Plowman, 1979). There are no germplasm collections or formalized breeding programs (Chung & Brink, 1999) which, in conjunction with active attempts to control the illicit market, makes the genetic diversity within these populations vulnerable to loss (Kangas, 1990).
Crop-to-wild gene flow in this group is complicated by a number of factors. Whereas E. coca var. coca is found as both apparently uncultivated populations and large plantations of cultivated plants, the other three taxa are only found as cultivated plants (and sometimes at smaller scale than the former) (Plowman, 1979). Therefore, gene flow from crops to non-crops is only possible when the recipient is uncultivated E. coca var. coca. Yet, feral and cultivated plants are perhaps impossible to distinguish --E. coca var. coca plantations may be abandoned and continue to survive in untended gardens which could be difficult to differentiate from seeds that have unintentionally grown in uncultivated areas (Plowman, 1979). Furthermore, there is little opportunity for varieties and species to exchange genes because their ranges rarely overlap and they have distinct ecological requirements (Plowman, 1979).
When Erythroxylum species do share habitats, varieties can interbreed, at least with moderate success (i.e., var. coca with var. ipadu and vice versa; var. novogranatense with var. truxillense and vice versa) (Bohm et al., 1982). Although occasional interspecific F| hybrids can be produced, they appear to be sterile or nearly so (Bohm et al, 1982; Chung & Brink, 1999), suggesting physiological reproductive barriers have evolved (Plowman, 1979; Plowman, 1980). However, in regard to the influence of gene flow on the evolution of uncultivated Coca populations, more information is needed about population dynamics (e.g., effective population size, dispersal patterns), the mode of inheritance of key chemical pathways and the genetic variability of these genes, as well as the ecological role of E. coca in its natural landscape (summarized in Box 1).
We did not locate studies of the effects of cocaine on the survival and fecundity of natural herbivores of Erythroxylum. Yet, several lepidopteran larvae and fungi consume Erythroxylum species as food sources, sometimes as obligate herbivores (e.g., Blum et al., 1981; Sands et al., 1997). In fact, the use of lepidopteran and fungal species tolerant of cocaine have been proposed as means of biological control of cocaine plants (U.S. Congress, 1993). Clinical studies of the effects of cocaine (rather than fresh leaves) on laboratory rodents and humans indicate that there are deleterious effects on mammals. In particular, rats fed a low protein and high carbohydrate diet gained less weight if they also consumed coca leaves than controls (Burczynski et al., 1986). This is consistent with results from chronic cocaine administration to female house mice that exhibited slowed growth and development, delayed puberty, and decreased weaning weight of pups, relative to controls (Chen & Vandenbergh, 1994). Cocaine administration also temporarily suppressed the immune system of mice (Ou et al, 1989). That said, in rodents, short-term cocaine administration enhances flexible and goal-directed behavior in ever-changing environments, as well as psychomotor speed, whereas long-term cocaine exposure results in general cognitive impairment across various functions (Spronk et al., 2013).
Toxicity is always related to dosage, and the literature reporting the toxicity of the plants discussed in this review needs to be interpreted with the understanding that many animals, including humans, tolerate certain levels of consumption without exhibiting ill effects. In some cases, people misuse social drugs by consuming large amounts. The concentrations in social drugs can be far greater than in the plants, so herbivores that consume the green plants are less likely to absorb toxic quantities. Indigenous people who have a long history with particular inebriant plants usually use limited dosages for medical or spiritual practices (de Rios & Smith, 1977). For example, coca is used, apparently safely, in South America as a leaf tea or masticatory, but extracted cocaine is clearly a dangerously addictive substance (Nutt et al., 2010).
Box 1: Summary of Risk of Gene Flow from Coca to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspccific: differences between cultivated and uncultivated plants not well understood, so risk, if any. is unclcar. --Interspecific: Evidence of intcrfcrtility uncvaluated for many close Erythroxylum relatives, but hybrid sterility suggests very limited risk. * Risk of toxicity to animals: Laboratory animals often harmed, but risk to natural herbivores unevaluatcd. * Risk of gcnctic swamping: Hybrid sterility suggests very limited risk. * Risk of generation of invigorated weeds: Negligible.
Coffee--Coffea arabica L., C. canephora Pierre ex A. Froehner, C. Iiberica Bull, ex Hiern (Rubiaceae)
Coffee is produced from the seeds of Coffea arabica, and to a lesser extent, C. canephora and C. liberica (Davis et al., 2006). Caffeine biosynthesis in coffee is now well understood (Nagai et al., 2008). Caffeine content in C. arabica and C. canephora is quantitatively inherited and generally controlled by genes with additive effects, with different inheritance mechanisms controlling caffeine content in seeds vs. leaves (Priolli et al., 2008). In addition to the domesticated species, there are several uncultivated species that produce significant amounts of caffeine, primarily in low altitude west and central Africa, where the high-caffeine crops C. arabica, C. canephora, and C. liberica also originate, suggesting caffeine may be protecting these plants against indigenous herbivores in the region (Hamon et al., 2017).
Hybridization among Coffea species is well known. The three cultivated Coffea species (C. arabica, C. canephora, and C. liberica) hybridize spontaneously and can coexist in the same habitat (Lashermes et al., 2000: Davis et al., 2006: Gomez et al., 2016). Indeed, C. arabica is considered to be a naturally occurring species of hybrid origin as a well-known allotetraploid hybrid (Davis et al., 2006). Cultivated C. liberica can mate with wild, caffeine-free C. pseudozangnebariae and the trait for caffeine production has been documented to be transferred (Barre et al, 1998). However, knowledge is limited about the extent of hybridization in the genus.
None of the caffeine-producing Coffea species are considered weedy, so at least with respect to this alkaloid there are no "crop-wild-weed complexes". However, as noted previously, hybridization has generated crop-wild hybrid offspring that produce caffeine and caffeine is known to possess allelopathic properties (Sugiyama et al., 2016). In fact, given their morphological intermediacy between a cultivated species and another wild species, two coffee "species" have been interpreted as hybrid lineages derived from crop-wild hybridization (C. affinis = C. liberica x C. stenophylla, Chevalier, 1947; Stoffelen, 1998; C. bakossii = C. liberica x C. montekupensis, Davis et al., 2006). Moreover, the cultivated coffee species have even been documented to produce fertile inter-generic hybrids (Psilanthus ebracteolatus Hiem x Coffea arabica L.; Couturon et al., 1998; Lombello & Pinto-Maglio, 2003; Lombello & Pinto-Maglio, 2004). When C. liberica hybridizes with wild, caffeine-free species, including C. canephora and C. psendozangaebariae, [F.sub.2] offspring express caffeine in their tissues (Barre et al., 1998; Akaffou et al., 2012; Amidou et al., 2007). Although we are not aware of any Coffea species that has gone extinct as a result of crop-wild hybridizations (summarized in Box 2), we expect wild Coffea species to be relatively vulnerable to this event. Two caffeine-free species (C. salvatrix and C. pseudozanguebariae) can hybridize with the domesticated coffee species, which may make these wild species particularly vulnerable to genetic swamping. Of 103 Coffea species, 72 (ca. 70%) are threatened with extinction due to loss of habitat (Davis et al., 2006). Further, genetic variation within crop populations of (C. arabica) is relatively low (Wellman, 1961; Anthony et al., 2002) and this species has been assigned a "vulnerable" extinction threat globally (Davis et al., 2006; IUCN, 2017).
Caffeine biosynthesis occurs in immature leaves (especially those produced while the plant is flowering), upper part of the stem, flowers, and immature fruits (but is absent in cotyledons, lower stem, and root), and it is accumulated in the mature leaves (Kim et al., 2006; Ashihara et al., 2008; Wright et al., 2013). Caffeine can have diverse consequences for organisms that live near or consume the coffee plant and there are two hypotheses as to its adaptive role in coffee plants (Sano et al., 2013). Caffeine has toxic and allelopathic effects, directly restricting the growth and development of bacteria, fungi, arthropods, and plants (Kim et al., 2010) while supporting the establishment of young coffee seedlings (Baumann & Gabriel, 1984; Aerts & Baumann, 1994). Additionally, it has been hypothesized that endogenous plant defenses are indirectly stimulated or primed by caffeine through signaling pathways (Kim & Sano, 2008).
In some animals, at (naturally) low doses, caffeine tends to enhance memory retention and cognitive performance (Nehlig, 1999). For instance, when caffeine occurs in floral nectar, pollinator memories of receiving a reward are enhanced (Wright et al., 2013) and thus these flowers tend to receive more bee pollinator visitation (Thomson et al., 2015). Moreover, some insects (e.g., coffee berry borer, Hypothenemus hampei) can metabolize caffeine as a defense (Ceja-Navarro et al., 2015). However, some insects are clearly deterred from feeding on Coffea or caffeine. For instance, Oligonychus ilicis (Acari; Tetranychidae) is a pest of Cojfea canephora (Gentianales: Rubiaceae) and C. canephora genotypes that are resistant to this mite species actually create resistance through a non-caffeine-related mechanism (instead trypsin-like protease inhibitors seem to differentiate C. canephora genotypes that are resistant and susceptible; Silva et al., 2015). Caffeine and other leaf extracts sprayed on a coffee leaf encouraged physical movement of the green scale (Leucoptera coffeella, a coffee pest) and reduced their feeding on treated, relative to untreated, coffee leaves (Magalhaes et al., 2010; Fernandes et al., 2011; Fernandes et al., 2012). In contrast, caffeine stimulates egg-laying by the coffee leaf miner in coffee leaves (Magalhaes et al., 2008). Moreover, when several caffeine biosynthetic pathway genes were expressed in tobacco plants, the leaves were unpalatable to tobacco cutworms (Spodoptera litura) (Kim et al., 2006).
Box 2: Summary of Risk of Gene Flow from Coffee to Wild Relatives. * Risk of gene transfer to wild relatives --Intraspeeific: classification uncertain, but genetic interchange with some putative ancestors seems very likely --Interspecific: Substantial * Risk of toxicity to animals: Caffeine is a known toxin for many animals, and gene transfer is likely to affect natural herbivores * Risk of genetic swamping: High * Risk of generation of invigorated weeds: Low
Cola (kolanut)--Cola spp. (Sterculiaceae)
The genus Cola comprises about 125 species of evergreen trees of tropical lowland and montane forests of continental Africa. For millennia, people have chewed kolanuts, derived from C. nitida and C. acuminata, for their stimulating effects, due to the caffeine content (Somorin, 1973; Suzuki & Waller 1987; Atawodi et al., 2007). Occasionally C. anomala is also cultivated for similar purposes in the Cameroon highlands. This tree crop is naturally distributed along the west coast of Africa (Bodard, 1955). However, less is known about the ca. ten close wild Cola species in West Africa, despite their potential value for the genetic improvement of the crop (Hutchinson & Dalziel, 1958; Adebola, 2011). For instance, wild C. ballayi seeds appear to have the same properties as C. accuminata, when chewed. When land is cleared for development. Cola trees, regardless of the species, are often left standing but are treated as an unmanaged resource (Tachie-Obeng & Brown, 2004). There are also two field-based germplasm collections of the crop, maintained as living trees in Nigeria and Ghana (Adebola et al., 2002; Aburi Botanical Gardens, 2002).
Few papers, to our knowledge, explore the herbivore community of kolanut. The herbivores are diverse and change with developmental stage of the plants, and include seedling-specific pests (Gryllotalpa africana, Brachytrypes membranaceus, and Zonocerus variegatus), stem borers (Phosphorus virescens var. jansoni, var. nimbatus, and var. gabonator) and defoliators (Sylepta semilugens, S. polycymalis, S. retractalis, and Anaphe venata) along with frugivores (Balanogastris kolae, Sophrorhinus quadricristatus, S. insperatus, S. duvenoyi, S. kolae, S. pujoli, S. gbanjaensis, Ceratitis colae) (Daramola, 1974). Although some study of the biology of these pests has occurred and wild Cola species may act as a refuge for weevils when the crop plants are unavailable (e.g., Daramola & Taylor, 1975; Daramola, 1981), we did not locate analyses of the effect of Co/a-derived caffeine on non-human animals of any kind. Caffeine is found in dozens of plant species (including those producing coffee, tea. and mate, discussed in this paper), and probably is a natural anti-herbivore agent (see the discussion for coffee).
The functionally monoecious mating system of the kolanut species is unusual because although it possesses male and hermaphrodite flowers, the viable pollen from the hermaphrodites has been confirmed to be non-functional in self- and crosspollinations (Opeke, 1984). Cola species appear to be pollinated by animal vectors (Russel, 1955; Bodard, 1962). Flowering times of C. nitida and C. acuminata partially overlap, allowing for possible pollen transfer between them. Cola nitida flowers twice a year with a minor flowering event occurring in January and February and a major flowering event occurring between August-October. Cola acuminata only flowers between January and March (Adebola, 2011). The two cultivated species of Cola and some wild relatives (C. lateritia, C. ballayi, C. verticillata, C. gigantea) possess the same number of chromosomes (2n = 40) (Morakinyo, 1978; Adebola & Morakinyo, 2005). Pollinations between the two partially self-incompatible, crop species have been reported (Jacob 1980, Morakinyo & Olorode, 1984). The [F.sub.1] offspring were viable but did not produce fruit, possibly due to post-zygotic barriers to genetic exchange (Jacob, 1980). In contrast, C. nitida can hybridize with C. millenii (a wild species) to produce viable offspring (Morakinyo, 1995). We did not locate publications reporting the caffeine content of inter-specific hybrids (Box 3).
Box 3: Summary of Risk of Gene Flow from Cola to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspecific: Low --Interspecific: Low * Risk of toxicity to animals: Not measured but potentially possible since caffeine is toxic. * Risk of gcnetic swamping: Possible but not measured. * Risk of generation of invigorated weeds: Unknown
Ephedra--Ephedra spp. (Ephedraceae)
Ephedra comprises about three dozen shrubs of arid environments of the northern hemisphere and South America. The genus produces two key alkaloids, ephedrine and pseudoephedrine, which are methamphetamine analogues. A few of the species from Asia (E. sinica, E. equisetina, and E. intermedia) produce moderate to high quantities of ephedrine (Qazilbach, 1971; White et al., 1997; Zhu, 1998) (Other species produce additional secondary metabolites with known neuropharmacological activity; Caveney et al., 2001; Ellis, 2003). The biosynthetic pathway of ephedrine synthesis in Ephedra has been partly clarified (Grue-Sorensen & Spenser, 1994) but only the first two steps have been characterized in high-alkaloid producing E. sinica (Okada et al., 2008; Krizevski et al., 2010), the species typically grown for commercial purposes (Caveney et al., 2001). Expression of ephedrine appears to be both environmentally dependent (Kondo et al., 1999) and genetically controlled (Krizevski et al., 2010), although the gene(s) responsible for ephedrine production have not been identified.
When Ephedra species grow in the same location, they frequently hybridize via wind-dispersed pollen (Huang et al., 2005; Kitani et al., 2011; Wu et al., 2016). However, rates of pollen movement have not been documented and expression of ephedrine in hybrid offspring of high- and low-content species remain unstudied (summarized in Box 4). The seeds of Ephedra are dispersed by both small rodents and wind (Meyer, 2008), providing two routes by which volunteer populations could escape cultivation and evolve ferality. The alkaloids present in Ephedra provide protection from both UV radiation and herbivory (Caveney et al., 2001) and so may influence fitness, but this has not been studied. Only three species of insects are known to consume Ephedra spp. and these feed exclusively on the plant (Qiao & Zhang, 2002; Luo & Wei 2015a, 2015b; Wang et al., 2017), perhaps because of novel detoxification mechanisms that they possess.
Exposure to methamphetamines via breast milk significantly negatively impacted pup development in rats and resulted in poorer maternal care (Sevcikova et al., 2017). More broadly, extracts of Ephedra spp. stimulate the central nervous system and influence muscle contraction in vertebrates (Miao et al., 2011). The potential for this plant to influence the food web dynamics of desert ecosystems, by altering nutritional options for herbivores (Janzen et al., 1977; Anderson & Pater, 2000), requires study (Box 4).
Box 4: Summary of Risk of Gene Flow from Ephedra to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspecific: differences between cultivated and wild plants not well documented, so risk of gene exchange is unknown --Interspecific: Strong evidence of intcr-fcrtility between some species * Risk of toxicity to animals: Probable, but effcct on wild herbivores not evaluated * Risk of genetic swamping: Unknown * Risk of generation of invigorated weeds: Unknown
Khat (or Qat)--Catha edulis (Vahl) Forssk. ex Endl. (Celastraceae)
Khat is a slow-growing evergreen shrub or tree of the Horn of Africa and the Arabian Peninsula. Its leaves are harvested for their mildly stimulating effects (Kalix, 1991; Lemessa & Ababa, 2001). Fanners clonally propagate superior strains of khat (Lemessa & Ababa, 2001). Clones are, of course, genetically uniform, and such selections sometimes have reduced seed production (Lemessa & Ababa, 2001). When seeds are produced, the caruncular appendage aids the seed in traveling long distances via wind dispersal (Zhang et al., 2014). Although older reports recognize more than one species of Catha, the genus is widely considered today to be monotypic, and presumably hybridization of C. edulis with other species is not possible (Catha was once classified in Celastrus, and, were hybridization possible, it presumably would be with the latter genus). Accordingly, the chief concern, insofar as gene flow is concerned, is that domesticated strains high in cathinone could transfer their enhanced production to wild plants. Despite an extensive search, we did not find reports of hybridization between domesticated and wild khat or descriptions of the inheritance of cathinone, the drug produced by the plant (Box 5). However, most reports noted a paucity of biological information on C. edulis (Lemessa & Ababa, 2001; Islam et al., 2006) and a better understanding of its basic biology is required. Although the ecology of khat has not been intensively studied, the plant is important in preventing soil erosion in Ethiopia (Lemessa & Ababa, 2001), and control programs to curb khat production are expected to have a negative impact on soil ecosystems in these communities.
As with all potentially toxic social drugs, there is particular interest in assessing the consequences of vertebrate consumption. In particular, grazing mammals in dryland regions are especially dependent on shrubs such as khat, and cathinone may have evolved in Catha as protection against mammalian grazing. Highland goats, which typically consume low to moderate quality forage-based diets, were fed khat biomass leftover after cathinone extraction, and their growth was significantly better than goats that were not fed khat (Waffle et al, 2012). Moreover, when fed leftover khat biomass, Ogaden sheep possessed fewer sperm abnormalities relative to control diet sheep (Mekasha et al., 2008). These observations suggest that Catha provides superior nutrition to grazers when cathinone is removed. There is additional evidence of toxicity of cathinone. In mice, low doses of crude khat extract have an immune stimulating property, whereas higher doses lead to suppression of cellular immune response (Ketema et al., 2015a). When mice are administered khat extract, they exhibit more severe malaria complications (Ketema et al., 2015b), thyroid hyperactivity (Zaghloul et al., 2003), enhanced locomotor activity, reduced social interaction and impaired cognitive function (Bogale et al., 2016). Chromosomal aberrations were detected in mice administered khat extract, making them more likely to develop cancer (Al-Zubairi et al., 2008).
Box 5: Summary of Risk of Gene Flow from Domesticated Khat to Wild Khat. * Risk of gene transfer to wild relatives: --Intraspccific: High possibility of transfer from domesticated to wild strains of Catha edutis --Interspecific: A monotypic genus, probably incapable of exchanging genes with other species * Risk of toxicity to animals: Slight risk of enhanced toxicity in wild plants * Risk of genetic swamping: Unknown * Risk of generation of invigorated weeds: Negligible
Marijuana--Cannabis sativa L. (Cannabaceae)
Cannabis is usually recognized as having one species, C. sativa (Small, 2015, 2016, 2017). It produces over 100 terpenophenolic compounds called cannabinoids, most notable of which are inebriating [[DELTA].sup.9]-tetrahydrocannabinol (THC) and non-inebriating cannabidiol (CBD) (Mechoulam, 1970; Turner et al., 1980; Mechoulam, 2005; Pertwee, 2006). The qualitative inheritance of THC and CBD has been clarified (de Meijer et al., 2003; Sirikantaramas et al, 2004; Marks et al., 2009; Weiblen et al., 2015), and the capacity to produce these chemicals can be readily exchanged among fibre, oilseed and drug cultivars, and wild plants (Box 6). All populations appear capable of interbreeding freely, so genes controlling cannabinoid production can easily be transferred.
Cannabis produces prodigious quantities of pollen, which can be carried vast distances by wind (Small & Antle, 2003). Unfertilized female plants are usually employed for production of marijuana, so males are generally culled from dioecious marijuana populations or female clones are grown in isolation from pollen sources (Ohlsson et al., 1971; Pijlman et al., 2005). Female clones have become the norm for marijuana production in western countries, and these almost never produce seeds, except sometimes under stress or deliberately treated chemically to produce some male flowers (e.g., Mohan Ram & Sett, 1982). Clonal production is a relatively recent cultivation tool and, to date, both cultivated hemp (low-THC Cannabis) and marijuana plants have been sources of pollen that could serve to transfer traits from cultivated to uncultivated populations.
Gene flow from cultivated to unmanaged locations may also occur by seed dispersal in C. sativa (Small & Antle, 2003). Many hemp cultivars are monoecious (Van der Werf et al., 1996), so a single seed of such a biotype, distributed to an uncultivated area, could self-fertilize and found a new population. However, monoecious Cannabis suffers from inbreeding depression (Heslop-Harrison & Heslop-Harrison, 1969), and represents distinctly inferior material for founding a population grown outside of cultivation. Dioecious hemp cultivars and marijuana strains, by definition, produce male and female plants, and so generally both sexes would need to be co-located in the wild to start a new population. Alternatively, a single female plant might receive pollen from quite remote locations. Cannabis sativa does not seem to have specialized adaptations for seed dispersal, but animal vectors (particularly humans and birds) and water are known agents of distribution (Small & Antle, 2003). Domesticated seeds of C. sativa (whether selected for fiber, oilseed or drugs) have largely lost several critical adaptations that improve survival outside of cultivation: dormancy (to not genninate in the autumn and be killed by frost), shattering (that allows seeds to fall off the infructescence), and camouflage (hiding the fallen seeds from rodents and insects) (Small, 1974). Nevertheless, hemp escapes are estimated to lose their domesticated seed phenotype within 50 generations, re-evolving the adaptations facilitating survival outside of cultivation (Small, 1984).
Cannabis sativa has been domesticated largely for stem fiber in temperate Eurasia. Land races and cultivars from this region exhibit a cannabinoid profile that is dominated by CBD (with very low amounts of THC), a relatively low production of cannabinoids (of the order of 2% dry weight of female inflorescences), and photoperiodic adaptation to flower relatively quickly in response to shortening day length (Small et al., 2003). By contrast, C. sativa has been domesticated largely for euphoric qualities in semi-tropical Asia and in Africa; land races from this region exhibit a cannabinoid profile that is dominated by THC (depending on land race, CBD may be absent or present in substantial amounts), a relatively high production of cannabinoids (typically 5% or more dry weight of female inflorescences), and photoperiodic adaptation to flower relatively slowly in response to shortening day length (Small et al., 2003). Worldwide, it appears that unmanaged populations in C. sativa are derived from cultivated populations (there is no evidence of persistent, ancestral populations, even within Afghanistan and India, the centres of marijuana domestication) (Small, 2017).
Generally, Cannabis sativa is a successional and "weedy" species and, in nature, feral populations are commonly found in disturbed areas, tolerating little competition, perhaps because the species has very high needs for nitrogen-rich, moist (albeit well-drained) soil, and high levels of sunlight (Small et al., 2003). There is some speculation that some constituents of C. sativa have possible allelopathic roles (Inam et al., 1989; McPartland, 1997a; McPartland et al., 2000) but this area of study is under-developed. In temperate areas of the world (especially most of Europe, northern Eurasia, and North America north of Mexico) "wild" (i.e., feral) populations are likely escapes from fiber hemp cultivated in recent past centuries when the crop was indispensable for canvas and cordage needed for ships. Indeed, mapped feral populations in North America tend to cluster around historic areas of hemp production (Small et al., 2003; Hillig & Mahlberg, 2004). In temperate Eurasia, where fiber hemp has been cultivated for millennia, feral hemp is extremely widespread and, presumably as a result of the much longer period for adaptation to have evolved, occupies a much greater range of habitats (Small, 1984). In semitropical Asia and Africa, most feral populations appear to be escapes from domesticated marijuana plants, sharing their high relatively high THC content and photoperiodic adaptation to relatively long seasons (Small & Beckstead, 1973). However, as a rule feral plants have significantly lower THC content by comparison with local marijuana strains (Small & Beckstead, 1973), suggesting that the conditions that support feral populations or natural selection do not favor extremely high THC concentrations.
Since THC and/or CBD can dominate the resin of most plants of C. sativa, the transfer of genes to feral populations that increases their presence is of concern, so it is desirable to understand how these cannabinoids can affect herbivores. Cannabis sativa foliage and flowering parts are consumed by grazing mammals and a wide diversity of herbivorous invertebrates (Haney & Bazzaz. 1970; Haney & Kutscheid, 1975; McPartland, 1998; McPartland et al, 2000), but are remarkably resistant to significant damage, which is likely the result of anti-herbivorous chemicals, including the cannabinoids (Small, 2016). By contrast, cannabinoids are virtually absent from the seeds, which are subject to extensive bird consumption (Ross et al., 2000, but see Yang et al., 2017). While the cannabinoids are economically valued largely because THC is a euphoriant chemical, it is highly unlikely that inebriation of herbivores occurs when they consume living plant material. In the living plant, the cannabinoids are carboxylated (a-COOH moiety is attached to the molecule), and in this condition THC is not significantly psychoactive (heat, as in smoking or cooking, sunlight or storage decarboxylates the acidic form of THC in marijuana; Taura et al., 2007). Moreover, insects, the primary herbivores of Cannabis (McPartland, 1997b, 1998, 1999; McEno, 1998; McPartland et al., 2000), lack endocannabinoid receptors necessary for euphoria in humans (McPartland et al., 2001). Although one hypothesis for their presence in plant populations is that the cannabinoids act as an anti-herbivory mechanism especially against insects (Rothschild & Fairbairn, 1980), exactly how cannabinoids are protective is uncertain--possibly a combination of toxicity, repellent taste, and objectionable stickiness (Small, 2016).
Several studies have demonstrated an apparent ability to detect, indeed prefer, THC by some non-human organisms. Some bacteria that can colonize the interiors of C. sativa roots like endomycorrhizal fungi, are capable of discriminating among marijuana genotypes, showing preferences for some over others, based on the cannabinoids possessed (Winston et al., 2014). Ovipositing Pieris rapae (cabbage white butterfly) was offered leaves painted with extracts from THC-possessing and THCabsent C. sativa plants; the butterflies notably avoided ovipositing on leaves painted with the high-THC extract (Rothschild & Fairbairn, 1980). In contrast, Tiger moths (Arctia caja) have a poor ability to discriminate among C. sativa that differ in THC concentration. When given a controlled choice of consuming a high-THC, lethal genotype of C. sativa or a low-THC, non-lethal genotype, the moths often chose the former, lethal option (Rothschild et al., 1977). Although these experiments suggest that THC is the basis for behavior, it needs to be kept in mind that correlated chemicals, such as terpenes, might actually have been the causative stimulus.
Given the significance of marijuana, there have been very extensive studies of the harm and therapeutic benefits of the cannabinoids, mostly THC and CBD, to humans and laboratory rodents, and depending on state of health, age, individual responses, and other factors, both benefits and harms have been demonstrated (reviewed in Small, 2016). There are numerous reports of companion dogs being poisoned (occasionally fatally) from deliberate or accidental ingestion of high-THC cannabis preparations intended for human recreation (Donaldson, 2002). Swiss physician and alchemist Paracelsus (1493-1541) is famous for his statement "Everything is a poison. The difference between a poison and a medicine depends on the dose." The concentrations of cannabinoids (indeed, of any secondary compound) that are high enough to have health and ecological consequences for the millions of wild species is of course almost impossible to determine, but as suggested by the following paragraph, there is at least some evidence that C. sativa repels many animals.
Powdered material and extracts of C. sativa have been used as anti-feedants, repellents and insecticides (Bouquet, 1950; McPartland, 1997a, 1997b). Mukhtar et al. (2013) found that C. sativa is effective against nematodes. There are numerous such studies of the effects of crude preparations of cannabis on various classes of noxious organisms, but there is often insufficient evidence to attribute the effects to particular chemicals. "Bhang" is a traditional Asian intoxicating beverage made with chopped Cannabis foliage, but the leaves and floral parts are not palatable, and recipe books on preparing cannabis edibles almost never recommend consumption of such tissues. Although small amounts of herbal cannabis (i.e. leaves and stems) can be incorporated in the feed of ruminant livestock and horses, this is not recommended (EFSA Panel, 2011). However, hemp silage is more acceptable (Letniak et al., 2000), the fermentation associated with silage preparation presumably neutralizing harmful constituents. The extent (if any) to which the cannabinoids in the previous examples are acting as a toxin remains undetermined (Box 6; Fig. 1).
Box 6: Summary of Risk of Gene Flow from Cannabis to Wild Relatives. * Risk of gene transfer: --Intraspecific: Extremely high risk to ruderal populations; ancestral wild populations, if extant, have not been identified. --Interspecific: Wild spccies of Cannabis have not been identified. * Risk of toxicity to animals: Seeds do not accumulate cannabinoids and are very palatable; foliage is toxic to numerous animals, almost all of which avoid consumption. * Risk of genetic swamping: Wild relative likely extinct due to genetic swamping. * Risk of generation of invigorated weeds: High.
Mate (Yerba Mate)--Ilex paraguariensis A. St. Hil. (Aquifoliaceae)
A small southern South American tree, Ilex paraguariensis, is the basis of a $1-billion beverage industry in Argentina, Brazil and Paraguay (Small & Catling, 2001; FAOSTAT, 2007; Grigioni et al., 2004; USDA ARS National Genetic Resources Program. 2007). Ilex paraguariensis leaves are used to prepare a popular hot drink (yerba mate or chimarrao) and in the production of stimulating pharmaceuticals with derivative methylxanthines, including caffeine and theobromine (Ricco et al., 1995). The chief chemical of culinary interest is caffeine, and many of the considerations regarding toxicity discussed for coffee, tea and kolanut apply to mate. However, likely the other chemicals present are ecologically significant. The plant is known to be allelopathic (PIER. 2008).
This dioecious species is outcrossing (Gauer & Cavalli-Molina, 2000), insectpollinated (Ayub, 1999), and flowers between October and November (Heck & de Mejia, 2007). There are 35 relatively close wild relatives (Vincent et al., 2013), Hao et al. (2013) stated "Hybridization and introgression events between distantly related lineages are inferred, indicating weak reproductive barriers between Ilex species," but our literature searches did not reveal any publications that tested sexual compatibility with I. paraguariensis or described putative hybrids with it. Therefore, we cannot assess the potential for gene flow from cultivated mate to related species. However, since breeding is occurring (discussed in the next paragraph) and cultivation is carried out within the natural wild distribution areas, it is inevitable that intraspecific gene flow will occur from cultivation to the wild. This is highly significant for conservation aspects of the species. IUCN (2017) provides an assessment of "near threatened" for I. paraguariensis, suggesting limited concern for the species' conservation status. However, according to PIER (2008), "the risks of genetic erosion are high because the natural forest is gradually giving way to agroforestry and livestock production." Under these circumstances, there should be considerable concern for future effects of introgression from domesticated to wild biotypes (Box 7).
Part of the mate commercial crop is harvested by extractive exploitation of the natural forest, which can result in a radical loss of genetic diversity within populations. Mate is also cultivated (Heck & de Mejia, 2007). Breeding programs are relatively recent and emphasize silvicultural characteristics, including plant architecture and its response to defoliation, leaf weight, and pest and disease resistance (Friedrich et al., 2017; Cardozo et al., 2010; Nakamura et al., 2009; de Resende et al., 2000; Wendt et al., 2007). There is evidence that insect herbivory is partly determined by ecological context (i.e., the local diversity of plant species) and the chemical content of plants (de Avila et al., 2016; Coelho et al., 2010).
Box 7: Summary of Risk of Gene Flow from Mate to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspecific: high, but, at present, domesticates arc scarcely different from wild forms, so gene transfer is likely inconsequential --Interspecific: Probable, but occurrence is unknown at present * Risk of toxicity to animals: Caffeine is a toxin so there is a possibility of increased toxicity in wild plants in the future as cultivars arc selected; risk is unmeasured at present * Risk of genetic swamping: Undetermined * Risk of generation of invigorated weeds: Negligible
Opium Poppy--Papaver somniferum L. (Papaveraceae)
There are about 100 species of Papaver, which are native mostly to temperate regions of the Northern Hemisphere (Goldblatt, 1974). Papaver somniferum, an annual herb, is believed to be indigenous to the Mediterranean region, from the Canary Isles eastwards (Goldblatt, 1974). It is now found as an escape from cultivation in fields, roadsides, and waste places in scattered localities throughout North America and in other regions of the world (Goldblatt, 1974). Papaver somniferum has been treated taxonomically in different ways. According to a popular interpretation, the cultivated phase is placed in subspecies somniferum while free-living plants are assigned to Subs/7, setigerum.
Opium poppies produce the global supply of medical-use morphine and illicit-use heroin, as well as the unharmful culinary poppy seed and some ornamental flowering cultivars (Williams, 2010). In fact, opium poppy (P somniferum) is the predominant source of several medicinal (and potentially toxic) alkaloids, notably morphine, thebaine. papaverine, codeine, narcotine (noscopine) and narceine (although it should be noted that several other species of Papaver produce some of the same compounds). Opium poppy produces almost 100 alkaloids (Hagel & Facchini, 2013), but, of these, morphine is infamous as the most deleterious narcotic to human welfare in history (Nutt et al., 2010). Some strains of P. bracteatum can produce considerable morphine, but are usually cultivated for codeine and thebaine (Hagel & Facchini, 2013). Crude opium is the hardened latex (milky sap) of the unripe fruit (capsule) of the opium poppy. The drug opium is a mixture of many constituents, but morphine is normally the most abundant alkaloid present; however, there are cultivars in which thebaine or codeine predominates (Lopez et al., 2018). Heroin, which is produced by chemical conversion of morphine, acts relatively rapidly to produce euphoria, and is a chief illegal drug of abuse (Nutt et al., 2010). The plant's capacity to produce pharmacological compounds is inherited and polygenic, controlled by at least three genetic regions, 4'-OMT (Ziegler et al., 2005), T60DM (Hagel & Facchini, 2010), and STORR (Winzer et al., 2015). Cultivare of opium poppy differ widely in ability to produce alkaloids; pharmaceutical cultivars produce much higher quantities of alkaloids than do culinary and ornamental varieties (Small, 2010). For instance, the capsules of some ornamental forms have less than 1% opiate alkaloids, while those of narcotic cultivars can have more than 20%. Curiously, although ornamental and ruderal populations contain opiates and are common, they are almost always legally tolerated (Lim, 2013; Meos et al., 2017). In recent times, medicinal cultivars (sometimes known by the oxymoron "low-morphine opium poppy") have been bred which are virtually devoid of "narcotic" constituents (Small, 2010).
Although generally characterized as self-pollinating (Tetenyi, 1977; Chitty et al., 2003), the outcrossing rate of P. somniferum can vary widely in the presence of insect pollen vectors, especially bees (7-71%; Chitty et al., 2003; Kumar & Patra, 2010). Opium poppy seeds are tiny--about 1.25-1.50 mm long--and are distributed by wind, which shakes the seeds out of the capsule through pores at the apex (Small, 2006). Such natural dispersal is usually restricted--up to 15 m (van der Pijl, 1982; Hughes et al., 1994; Vigni & Melati, 1999). The seeds of opium poppy are very nutritious, and because poppy seed is a widespread culinary item that is obtained from a plant which is considered dangerously narcotic, the issue of whether the seeds contain opiate alkaloids has been contentious. Trace amounts of alkaloids have been found in the embryos, but more significantly, since the seeds come from the capsules that are the source of opiates, contamination is almost unavoidable, although quality control can produce seeds with insignificant opiates. In nature, however, herbivores interested only in the seeds are likely to also consume opiates. Seeds can be transported by grazing animals which consume the seed pods and later defecate viable seeds (e.g., red deer (Cervus elaphus, Gill & Beardall, 2001), fallow deer (Dana dama, Claridge et al., 2016), European rabbit (Oryctolagus cuniculus, Fernandez & Saiz, 2007). Even if plant cultivation ceased in a site, the seeds of P. somniferum can remain viable yet donnant for up to a decade (Nagel & Borner, 2010). The distribution of feral P. somniferum is widespread but sparsely distributed in temperate regions (evidenced by several online floras: Kartesz, 2011; GBIF Secretariat, 2017; NBN, 2017; State Herbarium of South Australia, 2017; USDA-NRCS, 2017; Proto World Flora Online, 2017).
Opium poppy is sexually compatible with four closely related species (in particular, P. setigeram, but also P. bracteatum, P. orientale, and P. pseudo-orientale; Fig. 2a) (e.g., Duke, 1973; Ojala & Rousi, 1986; Garnock-Jones & Scholes, 1990). As noted previously, P. setigerum is often treated as a subspecies of P. somniferum (Small, 2006). Secondary gene transfer (i.e., transmission of genes from a source plant, via a bridging species, to a third, recipient species) may also occur since those compatible plants may hybridize with at least four additional species (Fig. 2a). Of course, successful gene flow depends on a shared geographic distribution (Fig. 2b). There are two regions of geographic overlap and thus potential hybridization hotspots (Central and Northern Germany, as well as the Swedish coast along the Baltic Sea in Europe and Turkey, Iran and along the Caucasus in West Asia), with a total of five species sharing a common range within areas of legal, large-scale cultivation (Fig. 2b, Chitty et al., 2003). However, the rate of gene flow and the propensity for secondary gene transfer are undetermined. Of ecological significance, experimental hybridization with P. somniferum resulted in increased morphine content in all recipients measured (Bohm & Nixdorf, 1983; Ojala & Rousi, 1986; Garnock-Jones & Scholes, 1990). Increased opiate availability can have a diversity of consequences for herbivores, including altering growth, reproductive success, health and cognitive function. Chickens fed 7.5-15% poppy seed meal consumed more food, grew larger, and produced heavier eggs with thicker eggshells but produced equivalent numbers of eggs relative to control-diet chickens (Kucukersan et al., 2009). In contrast, rat pups that were exposed to opiates through breastmilk had decreased body weights at birth and male oftspring had persistently reduced body weight (at weaning until 60 d of age) (Siddiqui et al., 1995). Morphine disrupted ovarian cyclicity in female rats and reduced fertilization rates; stillbirths were more common in morphine-exposed female rats (Siddiqui et al, 1995). Morphine-exposed male rats could copulate, but failed to impregnate females (Cicero et al., 2002). Generally, opiates have proved to be central nervous system depressants for most vertebrates assessed. They have increased anxiety in mice (Li et al., 2014) and impaired spatial working memory in rhesus monkeys (Wang et al., 2013). In contrast, horses experience central nervous system stimulation (Kollias-Baker & Sams, 2002). Race horses have been tested worldwide, often revealing low levels of opiate consumption, commonly attributed to environmental contamination of feed stock (Camargo et al., 2005). Occasionally, this contamination can be linked to seeds of P. somniferum (sometimes identified as P. setigenim) (Camargo et al, 2005). Opiates affect invertebrates in various ways, depending on the species; they suppress aggressive behaviour in crickets (Dyakonova et al., 2002) and immune function in aquatic invertebrates (Stefano et al., 1993). When exposed to morphine, rats exhibit abnormal immune function (Zhang et al., 2012) and rhesus monkeys (rhesus macaques) exhibit reduced immunocompetence (Carr & France, 1993). In nature, the influence of opiates may be transferred to higher trophic levels. Opiates have also been anecdotally reported to accumulate in honey when bees foraged near P. somniferum crops (McAlpine, 2002). Risk assessment is needed to establish the effect of increased environmental opiate contamination on ecological communities, and specifically on herbivores of P. somniferum and its hybrid offspring. (Figure 2)
Box 8: Summary of Risk of Gene Flow from Opium Poppies to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspeeifie: Very High Interspecific: High * Risk of toxicity to animals: High * Risk of genetic swamping: Substantial * Risk of generation of invigorated weeds: Low but not negligible
Tea--Camellia sinensis (L.) Kuntze, Camellia taliensis (W.W. Sm.) Melch. (Theaceae)
The genus Camellia is estimated to have about 300 species (Mondai, 2011). The tea plant (C. sinensis) is a small evergreen tree, usually pruned back to a low shrub in cultivation so that the leaves can be easily harvested. The species is native to the Assam-Burma-Yunnan triangle and has been planted widely in tropical and subtropical areas. Tea and coffee are the world's principal sources of caffeine beverages. Caffeine has been recorded in about 23 species of Camellia (Mondai, 2011). The commercial production of tea is largely based on harvesting the leaves of Camellia sinensis, although there is also local tea production from C. taliensis (Jackson, 1870; Zhao et al., 2014) and other species. Caffeine and several of its degradation products (especially allantoin and allantoate) accumulate in tissues of caffeine-containing plants such as coffee and tea, and in the case of tea these are found principally in the foliage, fruits, seeds, and flowers (Mohanpuria et al., 2009).
Meegahakumbura et al. (2016) stated that "since no wild populations of the tea plant have ever been found, the exact species used for the first domestication of tea plants remains unknown." Nevertheless, they identify China and to a lesser extent India as primary sites of domestication of C. sinensis. Many of its wild relatives are native to Southwest China (Yang et al., 2016). Natural intra-generic hybridization among C. sinensis and related species is a common occurrence (Sealy, 1958; Kotido, 1977; Takeda, 1990) and although [F.sub.1] hybrid offspring often have reduced fertility, it is frequently possible to generate [F.sub.2] offspring (Kondo, 1977). Although rarely measured, the capacity for caffeine biosynthesis can be transferred via inter-specific hybridization (e.g., between C. sinensis and another non-caffeine producing, horticulturally important plant, C. japonica-, Fujimori & Ashihara, 1990). However, we did not find any studies describing the prevalence of natural gene flow between the crop and its wild relatives, nor the consequences of such gene flow for the conservation of wild relatives (which are mostly identified as threatened by habitat loss) (e.g., Yang et al., 2011; IUCN, 2017; Box 9).
Feral or escaped tea trees are somewhat common within the geographic range of commercial production, especially when tea plantations are abandoned (Ahmed et al., 2010; SuZhen et al., 2011). However, we could not find significant research that suggested caffeine facilitates the persistence of these plants in unmanaged conditions. Perhaps, plants with higher caffeine may have an ecological advantage because caffeine exuded by tea seeds has been shown to have an allelopathic effect on neighbouring plants (Suzuki & Waller, 1987). Since tea plants can persist outside of cultivation, caffeine is probably frequently encountered in the agricultural landscape by herbivores and yet its impact on these organisms has rarely been measured. Beyond the broad effects of caffeine (described in the coffee section previously), tea caffeine has inhibited oviposition and delayed development in shot-hole borer beetle (Xyleborus fornicatus) under laboratory conditions (Hewavitharanage et al, 1999).
Box 9: Summary of Risk of Gene Flow from Tea to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspccific: very high --Interspecific: Medium * Risk of toxicity to animals: Likely affects a range of susceptible wild species * Risk of genetic swamping: Wild relative likely extinct * Risk of generation of invigorated weeds: Negligible
Tobacco--Nicotiana tabacum L.; N. rustica L. (Solanaceae)
Nicotine is a widespread alkaloid in plants, for example occurring in several cultivated members of the Solanaceae, such as tomato, potato, eggplant and green pepper (Siegmund et al., 1999) and in common milkweed (Asclepias syriaca) (Marion, 1939). Nicotine-based insecticides were frequently used worldwide until the 1980s, but have since lost popularity, in part because of concerns about their strong toxicity (Kozlowski et al., 2001). The wide-spectrum toxicity of nicotine coupled with its occurrence in unrelated plants indicates that it is a particularly potent natural deterrent of herbivores (see references later; Box 10).
The genus Nicotiana consists of about 44 species native to the Americas and 20 species native to Australia (Ren & Timko, 2000; Wylie et al., 2015). The most economically important species, common tobacco (Nicotiana tabacum), is an herbaceous annual (or less commonly a short-lived herb or forb). The species is thought to be a hybrid, the ancestors distributed in the eastern Andes Mountains of South America (Lewis & Nicholson, 2007). Tobacco from N. tabacum is produced in most temperate and tropical regions of the world, and is a major crop of many nations, with a global market worth over $112 billion annually (USDA, 2016). Nicotiana rustica is South American annual, naturalized in eastern North America (Gunn, 1974). Like N. tabacum, N. rustica was domesticated for tobacco production, although it is not grown commercially in North America. It is thought to have arisen as a hybrid, probably from ancestral species in Peru (Chase et al., 2003), and was spread northward as a result of cultivation by indigenous people (Gunn, 1974; Delcourt & Delcourt, 2004). Feral plants are rare, and when they are reported, these plants tend to be N. rustica which is not typically planted at an industrial scale like N. tabacum, von Gernet, 1992; Lim et al., 2004).
In the Solanaceae, nicotine is biosynthesized in the roots and transported to and accumulated in the foliage (Siegmund et al., 1999). The dried leaves of N. rustica contain up to 10% nicotine (younger leaves may have higher content), whereas those of N. tabacum usually have only 1.5--4% (although up to 8% is possible). Nicotine production is heritable and N. tabacum plants possess at least three nicotine demethylase genes that control nicotine production (Lewis et al., 2010). Research has documented inter-specific gene flow and alkaloid transfer, including nicotine, after experimental hybridizations, but success is low, as explained next (Smith. 1965).
Generally described as a self-pollinated plant, estimates of outcrossing rate for N. tabacum range from as low as 0.3% (Litton & Stokes, 1964) to as high as 17.2% (Paul et al., 1995). Hand crosses between N. tabacum and its closest relative, the ornamental tobacco (N. sylvestris) produced F| hybrids with extremely low pollen fertility and no seeds whereas back-cross progeny produced a few viable seeds (AlAhmad et al., 2006). Transgenic tobacco has become a major platform for pharmaceuticals (Small & Catling, 2006), and so there has been interest in establishing the extent to which gene escape from cultivated Nicotiana is possible (e.g., USDA, 2008). There have been limited tests of hybridization potential between N. tabacum and other wild relatives, such as N. tomentosiformis and N. otophora (Khoury et al., 2013), and to our knowledge there are no close weedy relatives. Because of the anti-herbivory properties of nicotine (Steppuhn et al., 2004) and the up-regulation of nicotine production in response to herbivory (Zong & Wang, 2004), it is plausible that the transfer of nicotine genes to low-nicotine N. tabacum populations could influence fitness of offspring. However, given the limited tendency to evolve ferality and low gene flow, even in sympatry, this is unlikely.
Increased nicotine consumption is known to have a diversity of consequences for herbivores, including altering growth, reproductive success, health and cognitive function.
Feeding on tobacco can increase the longevity of a phytophagous stilt bug (Jackson & Kester, 1996). In contrast, alcohol extracts of N. tabacum act as a repellent and toxicant against T. castaneum (red flour beetle) (Sagheer et al., 2013). Although some herbivorous insects (Myzus persicae: Cabrera-Brandt et al., 2010; Globodera tabacum tabacum: Lamondia, 1995) exhibit slowed or no reproduction on tobacco, others are clearly pests adapted to the crop. Moreover, increased tobacco in the environment may have trophic consequences. For instance, mirid parasitoid (Macrolophus caliginosus) exhibited faster maturity and more reproductive success when prey were raised on tobacco than cabbage or brussels sprouts (Hatherly et al., 2009). There also seem to be some health consequences to tobacco leaf exposure. Skin irritation in mice in response to tobacco leaves was attributed largely to the nicotine content of the leaves (Da Silva et al., 2010). Limited consumption of N. tabacum leaf did not affect sheep, but had anti-nematicidal effects on their parasitic nematodes (Hamad et al., 2013), and indeed tobacco is commonly employed topically to control ecto-parasites in dogs (Lansa et al., 2000). Although there is no evidence of tobacco-consumption affecting cognitive function in herbivores, there is some intriguing information from trials with nicotine extracts. For instance, nicotine-infused nectar sources, under experimental conditions, enhanced bee flower constancy (Baracchi et al., 2017).
Box 10: Summary of Risk of Gene Flow from Tobacco to Wild Relatives. * Risk of gene transfer to wild relatives: --Intraspecific: limited feral forms exist, with slight possibility of introgression to them --Interspecific: Low but possible in regions of South America where ancestral species persist * Risk of toxicity to animals: Nicotine is very toxic, and there is some possibility of increased nicotine in related wild species * Risk of genetic swamping: Unlikely, but study of potential target species required for evaluation of risk * Risk of generation of invigorated weeds: Negligible
Discussion of the Consequences of Gene Flow from Cultivated, Social Drug Plants
Considering the controversy over the health consequences of addictive social drug plants, it seems surprising that no one has yet presented a risk analysis of ecosystem consequences of their cultivation. From our review of the published literature, it is clear this science is in its infancy. Many cultivated, social-drug plants, including coffee, ephedra, khat, marijuana, mate, opium poppy, and tea, can exchange genes with either uncultivated intraspecific ancestors and/or close relatives. In fact, gene flow from cultivated, social drug plants to extant wild relatives is apparently rare only in self-pollinating tobacco and outcrossing kolanut. Although gene flow studies are limited for all of the species examined, further research is particularly required to document and measure gene flow from coca, ephedra and mate which appear to have no published information on this topic. Depending on rates of gene flow, genetic correlations, and adaptive trait values, genes that control social drug production and correlated traits could persist indefinitely in cultivated or free-living populations (Snow et al., 2005). These genes could continue to spread among other plants of the same species, especially if gene flow is maintained from a large source population, like an industrial cropping system.
Three issues regarding gene flow from addictive, social drug crops are of particular concern: (1) gene flow from social drug crops to their compatible plant relatives may increase the relative fitness of hybrid offspring; (2) it may endanger the conservation of genetic material that can be used for crop improvement in the future; (3) and it may influence food webs through altering the health of herbivores. These topics remain scarcely studied. Several of the plants reviewed produce illegal controlled substances, making it difficult to study them (Page & Ware. 2015). Nevertheless, as reviewed in this report, there is appreciable relevant information on the ecological and genetic impact of gene flow of several of the species.
Social drug plants are rarely weedy. Of the species examined, only marijuana expresses significantly weedy tendencies. Coffee and opium poppies show limited tendencies to escape cultivation, but they are not aggressive weeds. We found evidence to suggest that coca, khat, mate, tea, and tobacco do not tend to be weedy. There appear to be no studies on the weediness of kolanut and ephedra. Careful investigation comparing genotypes with and without drug properties would be interesting to determine if the social drug affects the ability of the plant to be more competitive or escape herbivory.
Wild relatives of crops represent invaluable sources of genes for crop improvement. Since ex situ conservation collections (gene seedbanks and permanent gardens) can only maintain a small fraction of natural gene pools, wild crop relatives are best maintained in situ (Husband & Campbell, 2004; Khoury et al., 2013). For instance, tobacco breeding has benefitted from the conserved germplasm of tobacco relatives that have received very little if any gene flow from crop tobacco. However, wherever crops are cultivated near their sexually compatible wild relatives, there is concern about gene escape from the crop and consequent alteration of gene frequencies in the wild forms (Snow et al., 2005; Campbell et al., 2016; Ellstrand et al., 2014); the most threatening possibilities are extinction of unique genes, extinction of unique populations, extinction of infra-specific taxa, and extinction of species. Additional research to understand the potential implications of gene flow on the conservation of germplasm should be urgently undertaken for coca, kolanut, ephedra, khat, and mate.
Although gene transfer between a crop and distantly-related species can be invaluable for plant breeding, for the most part taxa that are very closely related to a crop are the conventional sources of wild genes (Khoury et al., 2013). Indeed, the ancestor or ancestors of a crop, if extant, are the predominant sources of useful genes. Typically, crops are entirely or substantially inter-fertile with their wild ancestors (Ellstrand, 2003; Ellstrand et al., 2014), and so wherever the crop is grown in the same locality as surviving ancestors, there is a strong possibility of crop genes introgressing and altering altering gene frequencies in the ancestral gene pool (Snow et al., 2005), which is very undesirable from the point of view of gene conservation (Campbell et al., 2016). Genetic swamping has likely occurred in the close relatives of coffee, kolanut, and opium poppy. The ancestors and/or close relatives of marijuana and tea are not identifiable, according to published literature, although nevertheless feral forms likely have unique genes meriting conservation and protection from gene flow.
Future breeding efforts in social drug plants will rely, in part, on the genetic reserves of wild relatives (Ellstrand, 2003). Unfortunately, many natural ecosystems and many of their wild plant species are in severe decline as a result of human activities. Social drug plants are often harvested to the point that natural supplies are threatened (e.g., peyote: Hernandez & Barcenas, 1995), and unfortunately illicit drugs are often cultivated in wild places, endangering the surrounding ecosystem (e.g., McSweeney et al., 2014). While many crops are expanding, particularly the social drug species reviewed in this paper, many of their wild relatives are declining. This means that gene survival of many crop relatives is doubly threatened--by drastic reduction in habitats and population sizes, and also by gene transfer. A third threat applies to several of the social drug plants reviewed in this paper, because they are classified as sources of illegal or controlled narcotics. In this circumstance, the associated stigma has often prevented the collection of in situ resources, and indeed has often stimulated programs of destruction of wild-growing populations (Plowman, 1980).
Finally, one possible consequence of gene flow from addictive social drug crops is an increase in exposure of animals to potentially toxic compounds. In this review, a wide array of studies demonstrate the consequences of exposure to and effects of social drug plants on animal health and behavior. Clearly, animals respond strongly to compounds in coffee, marijuana, opium poppy, tea, and tobacco (but seemingly demonstrated few negative responses to khat).
Toxicological and behavioral studies of the effect of leafy material from coca, kolanut, ephedra and mate on animals are particularly limited and need to be undertaken.
Ideally, the industrial-scale cultivation of social drug plants should have been preceded by scientific research on their ecological and evolutionary consequences to susceptible species and ecosystems. Given the scale at which various nations are authorizing or permitting the development of social drug plants (e.g., Potter et al, 2015; UNGASS, 2016), there should be concern about both local and global unintended ecological consequences, which may be profound. In countries that produce social drugs from plants, information about the geographic distribution, population ecology, herbivore dynamics, and reproductive biology of sympatric wild relatives and non-drug crops will be useful for evaluating the potential for gene escape and the ecological effects of these phytochemicals, much as one might proceed with evaluating crops with novel traits that are potentially ecologically harmful (Lu & Snow, 2005; Snow et al, 2005). This knowledge will assist in promoting the further development of plant biotechnology and the safe production of promising plant-based chemicals globally. Given that several of the species we have reviewed here are highly stigmatized, many governments have restricted research so tightly that very few scientists have been able to conduct the required research to assess their possible benefits and harms. Publicly accessible and scientifically rigorous assessments of environmental impact are essential for the longterm success of these emerging industries as an important source of chemicals with valuable pharmaceutical properties.
Acknowledgements The authors gratefully acknowledge the critical comments of A. A. Snow, and the funding support from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grants program (no. 402305-2011 to LGC) and Ryerson University (for a research fellowship to CB).
Aburi Botanic Gardens. 2002. Conservation and cultivation of medicinal plants in Ghana. Medicinal Plant Workbook Accessions data. March 2002. Aburi Botanic Gardens, Ghana.
Adebola, P. O. 2011. Cola. Pp 63-71. In: C. Kole (ed). Wild Crop Relatives: Gcnomic and Breeding Resources. Springer, Berlin.
Adebola, P. O. & J. A. Morakinyo. 2005. Chromosome numbers of four Nigerian spccies of Cola Schott. and Endlicher (Sterculiaccac). Silvac Genetica 54: 42-44.
Adebola, P. O., O. M. Aliyu & K. Badaru. 2002. Genetic variability studies in the gcrmplasm collection of kola (Cola nitida [Vent] Schott and Endlicher) in South Western Nigeria. Plant Genetic Resources Newsletter 132: 57-59.
Aerts, R. J. & T. W. Baumann. 1994. Distribution and utilization of chlorogcnic acid in Coffea seedlings. Journal of Experimental Botany 45: 497-503.
Agrawal, A. A. & K. Konno. 2009. Latex: a model for understanding mechanisms, ecology, and evolution of plant defense against herbivory. Annual Review of Ecology, Evolution and Systcmatics 40: 311-331.
Ahmed, S., U. Unachukwu, J. R. Stepp, C. M. Peters, C. Long & E. Kennedy. 2010. Pu-erh tea tasting in Yunnan, China: correlation of drinkers' perceptions to phytochcmistry. Journal of Ethnopharmacology 132: 176-185.
Akaffou, P. D. S., P. Hamon, S. Doulbeau, J. Keli, H. Legnate, C. Campa, S. Hamon, A. de Kochko & B. I. A. Zoro. 2012. Inheritance and relationship between key agronomic and quality traits in an interspecific cross between Coffea pseudozanguebariae Bridson and C. canephora. Tree Genetics and Genomes 8: 1149-1162.
Al-Ahmad, H., S. Galili & J. Gressel. 2006. Infertile interspecific hybrids between transgenically mitigated Nicotiana tabaatm and Nicotiana sylvestris did not backcross to N. sylvestris. Plant Sciences 170: 953-961.
Al-Zubairi, A., P. Ismail, C. P. Pei & A. Rahmat. 2008. Genotoxic effect of Catha edulis (khat) crude extract after sub-chronic administration in rats. Environmental Toxicology and Pharmacology 25: 298303.
Amidou, N., N. Michel, H. Serge & P. Valerie. 2007. Gcnetic basis of species differentiation between Coffea liberica Hicrn and C. canephora Pierre: analysis of an interspecific cross. Genetic Resources and Crop Evolution 54: 1011-1021.
Anderson, M. K. & M. Pater. 2000. Plant Guide. Green ephedra: Ephedra viridis Cov. United States Department of Agriculture, National Resources Conservation Service, Washington D.C., USA.
Ankley, G. T., B. W. Brooks, D. B. Huggett & A. J. P. Sumpter. 2007. Repeating history: pharmaceuticals in the environment. Environmental Science and Technology 41: 8211-8217.
Anthony, F., M. Combes, C. Astorga, B. Bertrand, G. Graziosi & P. Lashermes. 2002. The origin of cultivated Coffea arabica L. varieties revealed by AFLP and SSR markers. Theoretical and Applied Genetics 104: 894-900.
Ashihara, H. & A. Crozier. 2001. Caffeine: a well-known but little mentioned compound in plant science. Trends in Plant Science 6: 407-413.
Ashihara, H., H. Sano & A. Crozier. 2008. Caffeine and related purine alkaloids: biosynthesis, catabolism, function and genetic engineering. Phytochemistry 69: 841-856.
Atawodi, S. E., B. Pfundstein, R. Haubner, B. Spiegelhaider, H. Bartsch & R. W. Owen. 2007. Content of polyphenols compounds in the Nigerian stimulants Cola nitida ssp. alba, Cola nitida ssp. nibra A. Chcv, and Cola acuminata Schott and Endl. and their antioxidant capacity. Journal of Agricultural and Food Chemistry 55: 9824-9828.
Avub, D. M. 1999. Biologia floral dc Ilex paraguariensis St. Hil. (erva-mate). M.Sc. thesis. PPG in Botany, UFRGS, Porto Alegre, Brazil.
Baldwin, I. T. 2001, An ecologically motivated analysis of plant-hcrbivorc interactions in native tobacco. Plant Physiology 12: 1449-1458.
Baracchi, D., A. Marples, A. J. Jenkins, A. R. Leitch & L. Chittka. 2017. Nicotine in floral nectar pharmacologically influences bumblebee learning of floral features. Scientific Reports 7: 1951.
Barre, P., S. Akaffou, J. Louarn, A. Charrier, S. Hamon & M. Noirot. 1998. Inheritance of caffeine and heteroside contents in an interspecific cross between a cultivated coffee species Coffea liberica var. dewevrei and a wild species caffeine-free C. pseudozanguebariae. Theoretical and Applied Genetics 96: 306-311.
Baumann, T. W. & H. Gabriel. 1984. Metabolism and excretion of caffeine during germination of Coffea arabica L. Plant and Cell Physiology 25: 1431-1436.
Bieri, S., A. Brachet, J. L. Veuthey & P. Christen. 2006. Cocaine distribution in wild Ervthroxylum specics. Journal of Ethnopharmacology 103: 439-447.
Blum, M. S., L. Rivier & T. Plowman. 1981. Fate of cocainc in the lymantriid Eloria noyesi, a predator of Ervthroxylum coca. Phytochemistry 20: 2499-2500.
Bodard, M. 1955. Contributions a l'ctude de Cola nitida, croissance et biologie florale. Centre Recherches Agronomique Bingerville Bulletin Bingerville 11: 3-28.
Bodard, M. 1962. Contributions a l'ctude systematique sure le Cola en Afrique occidentale. Universite dc Dakar Annales dc la Faculte des Lettres et Sciences Humaines 7: 71-82.
Bogale, T., E. Engidawork & E. Yisma. 2016. Subchronic oral administration of crude khat extract (Catha edulis Forsk) induces schizophernic-like symptoms in mice. BMC Complementary and Alternative Medicine 16: 153.
Bohm, B. A., F. R. Ganders & T. Plowman. 1982. Biosystematics and evolution of cultivated coca (Erythroxylaccac). Systematic Botany 7: 121-133.
Bohm, B. A. & H. Nixdorf. 1983. Quality and quantity of morphinan alkaloids detectable in inter-specific hybrids of the genus Papaver. Planta Medica 48: 193-204.
Bouquet, R. J. 1950. Cannabis. Bulletin on Narcotics 2: 14-30.
Briones, A., F. Cumsillc, A. Henao & B. Pardo (eds). 2013. The drug problem in the Americas. Organization of American States. Washington DC, USA.
Burczynski, F. J., R. L. Boni, J. Erickson & T. G. Vitti. 1986. Effect of Erythroxylum coca, cocaine and ecgonine methyl ester as dietary supplements on energy metabolism in the rat. Journal of Ethnopharmacology 16: 153-166.
Burgess, K. S., M. Morgan, L. Deverno & B. C. Husband. 2005. Asymmetrical introgression between two Moms species (M alba, M. rubra) that differ in abundance. Molecular Ecology 14: 3471-3483.
Cabrera-Brandt, M. A., E. Fuentes-Contreras & C. C. Figueroa. 2010. Differences in the detoxification metabolism between two clonal lineages of the aphid Myzus persicae (Sulzer) (Hemiptera: Aphididae) reared on tobacco (Nicotiana tabacum L.). Chilean Journal of Agricultural Research 70: 567-575.
Cadena-Gonzalez, A. L., M. Sorensen & 1. Theilade. 2013. Use and valuation of native and introduced medicinal plant species in Campo Hermoso and Zetaquira. Boyaca, Colombia. Journal of Ethnobiology and Ethnomedicinc 9: 23.
Camargo, F. C., A. F. Lehner, W. Karpiesiuk, K. Stirling, P. V. Kavanagh, N. Brennan, M. Dowling & T. Tobin. 2005. Review of environmental morphine identifications: worldwide occurrences and responses of authorities. Pp 58-64. In: Proe 51st Annual Convention of the American Association of Equine Practitioners. Seattle. Washington.
Campbell, L. G. & A. A. Snow. 2007. Competition alters life-history traits and increases the relative fecundity of crop-wild hybrids (Raphanus spp.). New Phytologist 173: 648-660.
Campbell, L. G., A. A. Snow & C. E. Ridley. 2006. Weed evolution after crop gene introgression: greater survival and fecundity of hybrids in a new environment. Ecology Letters 11: 1198-1209.
Campbell, L. G., D. Lee, K. Shukla, T. A. Waite & D. Bartsch. 2016. An ecological approach to measuring the evolutionary conscquenccs of gene flow from crops to wild or weedy relatives. Applications in Plant Science 4: 1500114.
Cardozo Jr., E. L., C. M. Donaduzzi, O. Ferrarese-Filho, J. C. Friedrich, A. Gonela & J. A. Sturion. 2010. Quantitative gcnctic analysis of mcthylxanthincs and phenolic compounds in mate progenies. Pesquisa Agropecuaria Brasileira 45: 171-177.
Carr, D. J. J. & C. P. France. 1993. Immune alterations in morphine-treated Rhesus monkeys. Journal of Pharmacology and Experimental Thcrapcutics 267: 9-15.
Caveney, S., D. A. Charlet, H. Freitag, M. Maier-Stolte & A. N. Starratt. 2001. New observations on the secondary chemistry of world Ephedra (Ephedraccae). American Journal of Botany 88: 1199-1208.
Ceja-Navarro, J. A., F. E. Vega, U. Karaoz, Z. Hao, S. Jenkins, H. C. Lim, P. Kosina, F. Infante, T. R. Northen & E. L. Brodie. 2015. Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee. Nature Communications 6: 7618.
Chen, C. J. & J. G. Vandenbergh. 1994. Effect of chronic cocaine on reproduction in female house mice. Pharmacology Biochemistry and Behavior 48: 909-913.
Chase, M. W., S. Knapp, A. V. Cox, J. J. Clarkson, Y. Butsko, J. Joseph, V. Savolainen & A. S. Parokonnv. 2003. Molecular systematics. GISH and the origin of hybrid taxa in Nicotiana (Solanaceae). Annals of Botany 92: 107-127.
Chevalier, A. 1947. Les cafeiers du globe, fase. 3: systematique des cafeiers et faux-cafeiers maladies et insectes nusibles. Encyclopedie Biologique 28: 1 -352.
Chitty, J. A., R. S. Allen, A. J. Fist & P. J. Larkin. 2003. Gcnctic transformation in commercial Tasmanian cultivars of opium poppy. Papaver somniferum, and movement of transgenic pollen in the field. Functional Plant Biology 30: 1045-1058.
Chung, R. C. K. & M. Brink. 1999. Erythroxylum (Eiythroxylaceae). Pp 259-262. In: L. S. dc Padua. N. Bunyapraphatsara and R. H. M. J. Lemmons (eds). Plant Resources of South East Asia No. 12: Medicinal and Poisonous Plants I. Backhuy Publisher. Leiden.
Cicero, T. J., L. A. Davis, M. C. LaRegina, E. R. Meyer & M. S. Schlegel. 2002. Chronic opiate exposure in the male rate adversely affects fertility. Pharmacology Biochemistry and Behavior 72: 157-163.
Claridge, A. W., R. Hunt, P. H. Thrall & D. J. Mills. 2016. Germination of native and introduced plants from scats of fallow deer (Dama dama) and eastern grey kangaroo (Macropus giganteus) in a southeastern Australian woodland landscape. Ecological Management and Restoration 17: 56-62.
Close, D. C. & C. McArthur. 2002. Rethinking the role of many plant phenolics-protection from photodamage not herbivores? Oikos 99: 166-172.
Coelho, G. C., S. B. Gnoatto, V. L. Bassani & E. P. Schenkel. 2010. Quantification of saponins in extractive solution of mate leaves (Ilex paraguariensis A. St. Hil.). Journal of Medicinal Food 13: 439-443.
Couturon, E., P. Lashermes & A. Charrier. 1998. First intergeneric hybrids (Psilanthus ebracteolattis Hiern X Coffea arabica L.) in coffee trees. Canadian Journal of Botany 76: 542-546.
Daly, T. W., J. Holmen & B. B. Fredholm. 1998. Is caffeine addictivc? The most widely used psychoactive substances in the world affects same parts of the brain as cocaine. Lakartidingen 95: 51-52.
Daramola, A. M. 1974. A review on the pests of Cola species in West Africa. Nigerian Journal of Entomology 1: 21-29.
Daramola, A. M. 1981. The biology of the kola weevil Balanogastris kolae on Cola acuminata and Cola verticillata. International Journal of Tropical Insect Science 2: 201- 204.
Daramola, A. M. & T. A. Taylor. 1975. Studies on the reinfestation of kola in store by kola weevils in Southern Nigeria. Journal of Stored Products Research 11:61-63.
Da Silva, F. R., B. Erdtmann, T. Dalpiaz, E. Nunes, D. P. Da Rosa, M. Porawski, S. Bona, C. F. Simon, M. D. Allgaver & J. Da Silva. 2010. Effects of dermal exposure to Nicotiana tabacum (Jean Nicot. 1560) leaves in mouse evaluated by multiple methods and tissues. Journal of Agricultural and Food Chemistry 58: 9868-9874.
Davis, A. P., R. Govaerts, D. M. Bridson & P. Stoffelen. 2006. An annotated taxonomic conspectus of the genus Coffea (Rubiaccac). Botanical Journal of the Linncan Society 152: 465-512.
de Avila, R. S., D. F. Dalazen, L. H. Lorentz, I. Poletto & V. M. Stefenon. 2016. Effects of different cultivation systems in leaf traits and herbivory damage in Ilex paraguariensis (Aquifoliaccac). Brazilian Journal of Botany 39: 219-223.
de Meijer, E. P. M., M. Bagatta, A. Carboni, P. Crueitti, V. Moliterni, P. Ranalli & G. Mandolino. 2003. The inheritance of chemical phenotype in Cannabis saliva L. Genetics 163: 335-346.
de Rios, M. D. & D. E. Smith. 1977. The function of drag rituals in human socicty: continuities and changes. Journal of Psychedelic Drugs 9: 269-275.
Delcourt, P. A. & H. R. Delcourt. 2004. Prehistoric Native Americans and ecological change: human ecosystems in eastern North America since the Pleistocene. Cambridge University Press.
Donaldson, C.W. 2002. Marijuana exposure in animals. Veterinary Medicine 97: 437-439. Duke, J. A. 1973. Utilization of Papaver. Economic Botany 27: 390-400.
Dyakonova, V., F. W. Schiirinann & D. A. Sakharov. 2002. Effects of opiate ligands on intraspecific aggression in crickets. Peptides 23: 835-841.
EFSA Panel on Additives and Products or Substances Used in Animal Feed. 2011. Scientific opinion on the safety of hemp (Cannabis genus) for use as animal teed. European Food Safety Authority Journal 9: 1-36.
Ellis, L. 2003. Archaeological method and theory: an encyclopedia. Taylor & Francis, New York, New York.
Ellstrand, N. C. 2003. Dangerous liaisons? When cultivated plants mate with their wild relatives. Johns Hopkins University Press, City. State.
Ellstrand, N. C. & K. A. Schierenbeck. 2000. Hybridization as a stimulus for the evolution of in vasivencss in plants? Proceedings of the National Academy of Sciences 97:7043-7050.
Ellstrand, N. C., P. Meirmans, J. Rong, D. Bartsch, A. Ghosh, T. J. de Jong, P. Haccou, B.-R. Lu, A. A. Snow, C. N. Stewart Jr, J. L. Strasburg, P. H. van Tienderen, K. Vrieling & D. Hooftman. 2014. Introgrcssion of crop alleles into wild or weedy populations. Annual Review of Ecology, Evolution and Systematics 44: 325-345.
FAO (Food and Agricultural Organization). 2004. Document #134. Trade in medicinal plants. Commodities and Trade Division of the Economic and Social Department of the FAO.
FAOSTAT (FAO Statistics Division). 2007. Available from: http://faostat.fao.org/site/408/DesktopDefault. aspx?PageID-408. Accessed Oct 31. 2017.
Fernandez, A. & F. Saiz. 2007. The European rabbit (Oiyctolagus cuniculus L.) as seed disperser of the invasive opium poppy (Papaver somniferum L.) in Robinson Crusoe Island. Chile. Mastozoologia Neotropical 14: 19-27.
Fernandes, F. L., M. C. Picanco, P. C. Gontijo, M. E. S. Fernandes, E. J. Guedes Pereira & A. A. Semeao. 2011. Induced responses of Coffea arabica to attack of Coccus viridis stimulate locomotion of the herbivore. Entomologia Experimentalis et Applicata 139: 120-127.
Fernandes, F. L., M. C. Picanco, M. E. S. Fernandes, R. B. Queiroz, V. M. Xavier & H. E. P. Martinez. 2012. The effects of nutrients and secondary compounds of Coffea arabica on the behavior and development of Coccus viridis. Environmental Entomology 41: 333-341.
Fowler, M. E. 1983. Plant poisoning in free-living wild animals: a review. Journal of Wildlife Diseases 19: 34-43.
Friedrich, J. C., A. Gonela, M. C. Goncalves Vidigal, P. S. Vidigal Filho, J. A. Sturion & E. L. Cardozo Jr. 2017. Genetic and phytochemical analysis to evaluate the diversity and relationships of mate (Ilex paraguariensis A.ST.-HIL.) elite genetic resources in a germplasm collection. Chemistry and Biodiversity 14: e1600177.
Fujimori, N. & H. Ashihara. 1990. Adenine metabolism and the synthesis of purine alkaloids in flowers of Camellia. Phytochcmistry 29: 3513-3516.
Ganders, F. R. 1979. Hctcrostyly in Erythroxylum coca (Erythroxylaccac). Botanical Journal of the Linncan Society 78: 11-20.
Garcia-Alonso, M., E. Jacobs, A. Raybould, T. E. Nickson, P. Sowig, H. Willekens, P. van der Kouwe, R. Layton, F. Amijee, A. M. Fuentes & F. Tencalla. 2006. A tiered system for assessing the risk of genetically modified plants to non-target organisms. Environmental Biosafcty Research 5: 57-65.
Garnock-Jones, P. J. & P. Scholes. 1990. Alkaloid content of Papaver somniferum subsp. setigerum from New Zealand. New Zealand Journal of Botany 28: 367-369.
Gauer, L. & S. Cavalli-Molina. 2000. Genetic variation in natural populations of mate (Ilex paraguariensis A. St.-Hil., Aquifoliaccac) using RAPD markers. Heredity 6: 647-656.
GBIF Secretariat. 2017. GBIF Backbone Taxonomy. doi:https://doi.org/10.15468/39omei. Accessed on March 10, 2017, via http://www.gbif.org/species/2888439
Gill, R. M. A. & V. Beardall. 2001. The impact of deer on woodlands: the cffects of browsing and seed disnersal on vepetatinn structure and romnositicin. Forestry 74: 209-218.
Glare, T. R. & M. O'Callaghan. 2000. Bacillus thuringiensis: Biology, Ecology and Safety. John Wiley & Sons Ltd, Chichcster, UK.
Goldblatt, P. 1974. Biosystematic studies in Papaver section Oxytona. Annals of the Missouri Botanical Gardens 61: 264-296.
Gomez, C., M. Despinoy, S. Hamon, P. Hamon. D. Salmon, D. S. Akaffou, H. Legnate, A. de Kochko, M. Mangeas & V. Poncet 2016. Shift in precipitation regime promotes interspecific hybridization of introduced Coffea species. Ecology and Evolution 6: 3240-3255.
Grigioni, G., F. Carduza, M. Irurueta & N. Pensei. 2004. Flavour characteristics of Ilex paraguariensis infusion, a typical Argentine product, assessed by sensory evaluation and electronic nose. Journal of the Science of Food and Agriculture 84: 427-432.
Grue-Sorensen, G. & I. D. Spenser. 1994. Biosynthetic route to the Ephedra alkaloids. Journal of the American Chemical Society 116: 6195-6200.
Gunn, C. R. 1974. Seed characteristics of 42 economically important species of Solanaceae in the United States. No. 1471. US Department of Agriculture.
Hagel, J. M. & P. J. Facchini. 2010. Dioxygenascs catalyze the O-demethylation steps of morphine biosynthesis in opium poppy. Nature Chemical Biology 6: 273-275.
Hagel, J. M. & P. J. Facchini. 2013. Benzylisoquinolinc alkaloid metabolism--a century of discovery and a brave new world. Plant Cell Physiology 54: 647-672
Hamad, K. K., Z. Iqbal, Z. U. D. Sindhu & G. Muhammad. 2013. Anti-ncmaticidal activity of Nicotiana tabacum L. leaf extracts to control benzimidazole-resistant Haemonchus conforms in sheep. Pakistan Veterinary Journal 33: 85-90.
Hao, D., X. Gu, P. P. Xiao, Z. Liang, L. Xu & Y. Peng. 2013 Research progress in the phytochemistry and biology of Ilex pharmaceutical resources. Acta Pharmaccutica Sinica B 3: 8-19.
Hamon, P., C. E. Grover, A. P. Davis, J. J. Rakotomalala, N. E. Raharimalala, V. A. Albert, H. L. Sreenath, P. Stoffelen, S. E. Mitchell, E. Couturon, S. Hamon, A. de Kochko, D. Crouzillat, M. Rigoreau, U. Sumirat, S. Akaffou & R. Guyot 2017. Genotyping-by-sequencing provides the first well-resolved phylogeny for coffee (Coffea) and insights into the evolution of caffeine content in its species: GBS coffee phylogcny and the evolution of caffeinc content. Molecular Phylogenetics and Evolution 109: 351-361.
Haney, A. & F. A. Bazzaz. 1970. Some ecological implications of the distribution of hemp (Cannabis sativa L.) in the United States of America. Pp 39-48. In: C. R. B. Joyce and S. H. Curry (eds). The Botany and Chemistry of Cannabis. J. and A. Churchill, London. UK.
Haney, A. & B. B. Kutscheid. 1975. An ecological study of naturalized hemp (Cannabis sativa L.) in castcentral Illinois. American Midland Naturalist 93: 1-24.
Hartmann, T. 1999. Chemical ecology of pyrrolidine alkaloids. Planta 207: 483-495.
Haslam, E. 1986. Secondary metabolism--facts and fiction. Natural Product Reports 3: 217-249.
Hatherly, I., B. Pedersen & J. Bale. 2009. Effect of host plant, prey species and intcrgencrational changes on the prey preferences of the predatory mirid Macrolophus caliginosus. BioControl 54: 35-45.
Heck, C. I. & E. G. de Mejia. 2007. Yerba Mate tea (Ilex paraguariensis): a comprehensive review on chemistry, health implications, and technological considerations. Journal of Food Science 72: R138-R151.
Hegde, S. G., J. D. Nason, J. M. Clegg & N. C. Ellstrand. 2006. The evolution of California's wild radish has resulted in the extinction of its progenitors. Evolution 60: 1187-1189.
Hernandez, H. M. & R. T. Barcenas. 1995. Endangered cacti in the Chihuahuan Desert: I. distribution patterns. Conservation Biology 9: 1176-1188.
Heslop-Harrison, J. & Y. Heslop-Harrison. 1969. Cannabis sativa L. Pp 205-226. In L. T. Evans (cd). The induction of flowering. Some case histories. Cornell University Press. Ithaca, NY.
Hewavitharanage, P., S. Karunaratne & N. S. Kumar. 1999. Effect of caffeine on shot-hole borer beetle (Xyleborus fornicatus) of tea (Camellia sinensis). Phytochemistry 51: 35-41.
Hillig, K. W. & P. G. Mahlberg. 2004. A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaccae). American Journal of Botany 91: 966-975.
Hovick, S. M., L. G. Campbell, A. A. Snow & K. D. Whitney. 2012. Hybridization alters early life-history traits and increases plant colonization success in a novel region. American Naturalist 179: 192-203.
Huang, J., D. E. Giannasi & R. A. Price. 2005. Phylogenetic relationships in Ephedra (Ephedraccac) inferred from chloroplast and nuclear DNA sequences. Molecular Phylogenetics and Evolution 35: 4859.
Hughes, L., M. Dunlop, K. French, M. R. Leishman, B. Rice, L. Rodgerson & M. Westoby. 1994. Predicting dispersal spectra: a minimal set of hypotheses based on plant attributes. Journal of Ecology 82: 933-950.
Husband, B. C. & L. G. Campbell. 2004. Population genetic and demographic responses to novel environments: implications for ex situ plant conservation. Pp. 231-266. In: E. Guerrant Jr.. K. Havens & M. Maunder (eds). Ex situ plant conservation symposium: strategies for survival. Island Press, Washington, USA.
Hutchinson, J. & J. M. Dalziel. 1958. Flora of West Tropical Africa, 2nd cdn. Crown Agents, London, UK (Revised by R. W. J. Keay).
Hylander, K. & S. Nemomissa. 2009. Complementary roles of home gardens and exotic tree plantations as alternative habitats for plants of the Ethiopian montane rainforest. Conservation Biology 23: 400-409.
Iason, G. R. & J. J. Villalba. 2006. Behavioral strategies of mammal herbivores against plant secondary metabolites: the avoidance-tolerance continuum. Journal of Chemical Ecology 32: 11 15-1132.
Inam, B., F. Hussain & F. Bano. 1989. Cannabis sativa L. is allelopathic. Pakistan Journal of Scientific and Industrial Research 32: 617-620.
Islam, M. B., M. P. Simmons & R. H. Archer. 2006. Phytogeny of the Elaeodendron group (Celastraccae) inferred from morphological characters and nuclear and plastid genes. Systematic Botany 31:512-524.
IUCN. 2017. The IUCN Red List of Threatened Species. Version 2017-3. Available at: www.iucnrcdlist.org. (Accessed: 19 September 2017).
Jackson, J. R. 1870. Tea. Nature 2: 215-217.
Jackson, D. M. & K. M Kester. 1996. Effects of diet on longevity and fecundity of the spined stilt bug, Jalysus wickhami. Entomologia Experimentalis ct Applicata 80: 421-425.
Jacob, V. J. 1980. Pollination fruit setting and incompatibility in Cola nitida. Incompatibility Newsletter 2: 50-56.
Janzen D. H., H. B. Juster & E. A. Bell. 1977. Toxicity of secondary compounds to the seed-cating larvae of the bruchid beetle Callosobruchus maculatus. Phytochemistry 16: 223-227.
Jhala, A. J., D. Sarangi, P. Chahal, A. Saxena, M. Bagavathiannan, B. Singh Chauhan & P. Jha. 2017. Inter-specific gene flow from herbicide-tolerant crops to their wild relatives. Pp 87-122. In: M. Jugulam (ed). Biology, physiology and molecular biology of weeds. CRC Press, Boca Raton, FL.
Kadereit, J. W. 1986. Experimental evidence on the affinities of Papaver somniferum (Papaveraccae). Systematics and Evolution 156: 189-195.
Kalix, P. 1991. The pharmacology of psychoactive alkaloids from Ephedra and Catha. Journal of Ethnopharmacology 32: 201-208.
Kangas, P. 1990. Ecology and the war on drugs. Bulletin of the Ecological Society of America 71:105-111.
Kartesz, J. T. 2011. The biota of North America program (BONAP). North American Plant Atlas. Accessed March 10, 2017. Available at: http://www.bonap.org/
Ketema, T., M. Yohannes, E. Alemayehu & A. Ambelu. 2015a. Evaluation of immunomodulatory activities of methanolic extract of khat (Catha edulis, Forsk) and cathinone in Swiss albino micc. BMC Immunology 16: 9.
Ketema, T., M. Yohannes, E. Alemayehu & A. Ambelu. 2015b. Effect of chronic khat (Catha edulis, Forsk) use on outcome of Plasmodium berghei ANKA infection in Swiss albino mice. BMC Infectious Diseases 15: 170.
Khoury, C. K., S. Greene, J. Wiersema, N. Maxted, A. Jarvis & P. C. Struik. 2013. An inventory of cropwild relatives of the United States. Crop Science 53: 1496-1508.
Kiang, Y. T., J. Antonovics & L. Wu. 1979. The extinction of wild rice (Oryza perennis formosana) in Taiwan. Journal of Asian Ecology 1: 1-9.
Kitani, Y., S. Zhu, J. Batkhuu, C. Sanchir & K. Komatsu. 2011. Genetic diversity of Ephedra plants in Mongolia inferred from internal transcribed spacer sequence of nuclear ribosomal DNA. Biological Pharmacy Bulletin 34: 717-726.
Kim, Y. S., H. Uefuji, S. Ogita & H. Sano. 2006. Transgenic tobacco plants producing caffeine: a potential new strategy for insect pest control. Transgenic Research 15: 667-672.
Kim, Y. S. & H. Sano. 2008. Pathogen resistance of transgenic tobacco plants producing caffeine. Phytochemistry 69: 882-888.
Kim, Y. S., Y. E. Choi & H. Sano. 2010. Plant vaccination: stimulation of defense system by caffeine production in planta. Plant Signalling and Behavior 5: 489-493.
Kollias-Baker, C. & R. Sams. 2002. Detection of morphine in blood and urine samples from horses administered poppy seeds and morphine sulfate orally. Journal of Analytical Toxicology 26: 81-86.
Kondo, K. 1977. Cytological studies in cultivated species of Camellia. I. Diploid species and their hybrids. Japanese Journal of Breeding 27: 28-28.
Kondo, N., M. Mikage & K. ldaka. 1999. Medico-botanical studies of Ephedra plants from the Himalayan region, part III: causative factors of variation of alkaloid content in herbal stems. Journal of Natural Medicines 53: 194-200.
Kozlowski, L. T., J. E. Henningfield & J. Brigham. 2001. Cigarettes, nicotine, and health: A biobehavioral approach. Vol. 5. Sage Publishing.
Kucukersan, S., D. Yesilbag & K. Kucukersan. 2009. Using of poppy seed meal and yeast culture (Saccharomyces cerevisiae) as an alternative protein source for layer hens. Kafkas Universitesi Veteriner Fakultesi Dergisi 15: 971-974.
Krizevski, R., E. Bar, O. Shalit, Y. Sitrit, S. Ben-Shabat & E. Lewinsohn. 2010. Composition and stereochemistry of ephedrine alkaloids accumulation in Ephedra sinica Stapf. Phytochemistry 71: 895-903.
Kumar, B. & N. K. Patra. 2010. Gene frequency-based estimation of natural outcrossing in opium poppy (Papaver somniferum L.). Molecular Breeding 26: 619-626.
Lamondia, J. A. 1995. Hatch and reproduction of Globodera tabacum tabacum in response to tobacco, tomato or black nightshade. Journal of Nematology 27: 382-386.
Lansa, C., T. Harper, K. Georges & E. Bridgewater. 2000. Medicinal plants used for dogs in Trinidad and Tobago. Preventive Veterinary Medicine 45: 201-220.
Lashermes, P., S. Andrzejewski, B. Bertrand, M. C. Combes, S. Dussert, G. Graziosi, P. Trouslot & F. Anthony. 2000. Molecular analysis of introgressive breeding in coffee (Coffea arabica L.). Theoretical and Applied Genetics 100: 139-146.
Lemessa, D. & A. Ababa. 2001. Khat (Calha edulis): botany, distribution, cultivation, usage and economics in Ethiopia. United Nations Development Programme. Emergency Unit for Ethiopia.
Letniak, R., C. Weeks, S. Blade & A. Whiting. 2000. Low THC hemp (Cannabis sativa L.) Research Report 99-10028-R11999. Alberta Agriculture and Forestry, Hcmaruka, Alberta.
Levy, A. & J. Milo. 1991. Inheritance of morphological and chcmical characters in interspecific hybrids between Papaver bracteatum and Papaver pseudo-orientale. Theoretical and Applied Genetics 81: 537-540.
Lewis, R. S. & J. S. Nicholson. 2007. Aspects of the evolution of Nicotiana tabacum L. and the status of the United States Nicotiana germplasm collection. Genetic Resources and Crop Evolution 54: 727-740.
Lewis, R. S., S. W. Bowen, M. R. Keogh & R. E. Dewey. 2010. Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tabacum L.: Functional characterization of the CYP82E10 gene. Phytochemistry 71: 1988-1998.
Li, C. Q., Y. W. Luo, F. F. Bi, C.Q. Li, Y.VV. Luo, F. F. Bi, T. T. Cui, L. Song, W. Y. Cao, J. Y. Zhang, F. Li, J. M. Xu, W. Hao, X. W. Xing, F. H. Zhou, X. F. Zhou & R. P. Dai. 2014. Development of anxietylike behavior via hippocampal IGF-2 signaling in the offspring of parental morphine exposure: effect of enriched environment. Neuropsychopharmacology 39: 2777-2787.
Lim, T. K. 2013. Papawr somniferum. Pp 202-217. In: T. K. Lim. Edible medicinal and non-medicinal plants. Springer Netherlands. Chicago. USA.
Lim, K. Y., R. Matvasek, A. Kovarik & A. R. Leitch. 2004. Genome evolution in allotctraploid Nicotiana. Biological Journal of the Linnean Society 82: 599-606.
Litton, C. C. & G. VV. Stokes. 1964. Outcrossing in Burlcy tobacco. Tobacco Science 8: 113-115.
Liu, Y. B., H. Darmency, C. N. Stewart, W. Wei, Z. X. Tang & K. P. Ma. 2014. The effect of Sf-transgene introgression on plant growth and reproduction in wild Brassica juncea. Transgenic Research 24: 537-547.
Lombello, R. A. & C. A. F. Pinto-Maglio. 2003. Cytogenetic studies in Psilanthus ebracteolatus Hicm., a wild diploid coffee species. Cytologia 68: 425-429.
Lombello, R. A. & C. A. F. Pinto-Maglio. 2004. Cytogenetic studies in Coffea L. and Psilanthus Hook, using CMA/DAPI and FISH. Cytologia 69: 85-91.
Londo, J. P., N. S. Bautista, C. L. Sagers, E. H. Lee & L. S. Watrud. 2010. Glyphosate drift promotes changes in fitness and transgene gene flow in canola (Brassica napus) and hybrids. Annals of Botany 106: 957-965.
Lopez, P., D. P. K. H. Pereboom-de Famv, P. P. J. Mulder, M. Spanjer, J. de Stoppelaar, H. G. J. Mol & M. de Nijsa. 2018. Straightforward analytical method to determine opium alkaloids in poppy seeds and bakety products. Food Chemistry 242: 443-450.
Lu, B. R. & A. A. Snow. 2005. Gene flow from genetically modified rice and its environmental consequences. Bioscience 55: 669-678.
Lu, B. R., X. Yang & N. C. Ellstrand. 2016. Fitness correlates of crop transgene flow into weedy populations: a case study of weedy rice in China and other examples. Evolutionary Applications 9: 857-870.
Luo, C. & C. Wei. 2015a. Intraspccific sexual mimicry for finding females in a cicada: males produce 'female sounds' to gain reproductive benefit. Animal Behaviour 102: 69-76.
Luo, C. & C. Wei. 2015b. Stridiilatory sound-production and its function in females of the cicada Subpsaltria vangi. PLoS ONE 10: e0118667.
Maag, D., M. Erb, T. G. Kollner & J. Gershenzon. 2015. Defensive weapons and defense signals in plants: some metabolites serve both roles. BioEssays 37: 167-174.
Magalhaes, S.T.V., R. N. C. Guedes, A. J. Demuner & E. R. Lima. 2008. Effect of coffee alkaloids and phcnolics on egg-laying by the coffee leaf miner Leucoptera coffeella. Bulletin of Entomological Research 98: 483-489.
Magalhaes, S.T.V., F. L. Fernandes, A. J. Demuner, M. C. Picanco & R. N. C. Guedes. 2010. Leaf alkaloids, phcnolics, and coffee resistance to the leaf miner Leucoptera coffeella (Lcpidoptera: Lyonetiidac). Journal of Economic Entomology 103: 1438-1443.
Malik, C. P., T. N. Mary & I. S. Grover. 1979. Cytogenic studies in Papaver. V. Cytogenic studies on P. somniferum x P. setigerum hybrids and amphiploids. Cytologia 44: 59-69.
Marion, L. 1939. The occurrence of 1-nicotinc in Asclepias syriaca L. Canadian Journal of Research 17: 21-22.
Marks, M. D., L. Tian, J. P. Wenger, S. N. Omburo, W. Soto-Fuentes, J. He, D. R. Gang, G. D. Weiblen & R. A. Dixon. 2009. Identification of candidate genes affecting delta 9-tctrahydrocannabinol biosynthesis in Cannabis sativa. Journal of Experimental Botany 60:3715-3726.
McAlpine, A. 2002. Adventures of a Collector. Allen & Unwin, Crow's Nest, New South Wales.
MeEno, J. 1998. Cannabis ecology: a compendium of diseases and pests. Amrita Press. Middlcbury, VT.
McPartland, J. M. 1997a. Cannabis as repellent and pesticide. Journal of the International Hemp Association 4: 87-92.
McPartland, J. M. 1997b. Diseases and Pests of Cannabis. Pp 284-290. In: Nova Institute. Bioresource hemp--Proceedings of the Symposium (Frankfurt am Main, Germany, Feb. 27--March 2, 1997). Nova Institute, Hurth, Germany.
McPartland, J. M. 1998. Diseases and pests of hemp in Canada. Commercial Hemp Magazine 2: 33-34.
McPartland, J. M. 1999. A survey of hemp diseases and pests. Pp 109-131. In: P. Ranalli (ed). Advances in Hemp Research. Food Products Press, New York. NY.
McPartland, J. M., R. C. Clarke & D. P. Watson. 2000. Hemp Diseases and Pests: Management and Biological Control. CABI, New York, NY.
McPartland, J. M., V. Di Marzo, L. De Petrocellis, A. Mercer & M. Glass. 2001. Cannabinoid receptors arc absent in insects. Journal of Comparative Neurology 436: 423-429.
McSweeney, K., E. A. Nielsen, M. J. Taylor, D. J. Wrathall, Z. Pearson, O. Wang & S. T. Plumb. 2014. Drug policy as conservation policy: Narco-deforestation. Science 343: 489-490.
Mechoulam, R. 1970. Marihuana chemistry. Science 168: 1159-1166.
Mechoulam, R. 2005. Plant cannabinoids: a neglected pharmacological treasure trove. British Journal of Pharmacology 146: 913-915.
Meegahakumbura, M. K., M. C. Wambulwa, K. K. Thapa, M. M. Li, M. Moller, J. C. Xu, J. B. Yang, B. Y. Liu, S. Ranjitkar, J. Liu & D. Z. Li. 2016. Indications for three independent domestication events for the tea plant (Camellia sinensis (L.) O. Kuntze) and new insights into the origin of tea germplasm in China and India revealed by nuclear microsatellites. PLoS ONE 11: e0155369.
Mekasha, Y., A. Tegegne & H. Rodriguez-Martinez. 2008. Feed intake and sperm morphology in Ogaden bucks supplemented with either agro-industrial by-products or kliat (Catha edulis) leftover. Reproduction in Domestic Animals 43: 437-444.
Meos, A., L. Saks & A. Raal. 2017. Content of alkaloids in ornamental Papaver somniferum L. cultivars growing in Estonia. Proceedings of the Estonianian Academy of Sciences 64: 1-9.
Meyer, S. E. 2008. Ephedra. Pp 492-494. In: The Woody Plant Seed Manual. USDA Forest Service Agriculture Handbook 727.
Miao, Y. R., Q. H. Yang, S. Y. Yu, S. Q. Luo & Y Zhang. 2011. Research progress of ephedrine and pseudoephedrine. Journal of Inner Mongolia Medical College 33: 490-494. [with English abstract]
Mitchell, D. C., C. A. Knight, J. Hockenberrv, R. Teplanskv & T. J. Hartman. 2014. Beverage caffeine intakes in the U.S. Food and Chemical Toxicology 63: 136-142.
Mohanpuria, P., V. Kumar, R. Joshi, A. Gulati, P. S. Ahuja & S. K. Yadav. 2009. Caffeine biosynthesis and degradation in tea [Camellia sinensis (L.) O. Kuntzc] is under developmental and seasonal regulation. Molecular Biotechnology 43: 104-111.
Mohan Ram, H. Y. & R. Sett. 1982. Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulfate anionic complex. Theoretical and Applied Genetics 62: 369-375.
Mondai, T. K. 2011. Camellia. Pp 15-39. In: C. Kole (cd). Wild crop relatives: genomic and breeding resources. Springer-Verlag. Berlin.
Morakinyo, J. A. 1978. Biosystematics studies in the genus Cola Sehott and Endlicher. M.Sc. thesis, University of Ifc, Nigeria.
Morakinyo, J. A. 1995. Gene exchange between Cola millenii and Cola nitida, hybridization and seed viability. Bioscicncc Research Communication 7: 151 153.
Morakinyo, J. A. & O. Olorode. 1984. Cytogenetics and morphological studies on Cola acuminata (P. Beauv.) Schott and Endlicher., Cola nitida (Vent) Schott and Endlicher and the C. acuminata x C. nitida [F.sub.1] hybrid. The Cacao Cafe 28.
Mukhtar, T., M. Z. Kayani & M. A. Hussain. 2013. Nematicidal activities of Cannabis sativa L. and Zanthoxylum alatum Roxb. against Meloidogyne incognita. Industrial Crops and Products 42: 447-453.
Nagai, C., J. J. Rakotomalala, R. katahira, Y. Li, K. Yamagata & H. Ashihara. 2008. Production of a new low-caffcinc hybrid coffee and the biochemical mechanism of low caffeine accumulation. Euphytica 164: 133-142.
Nagel, M. & A. Borner. 2010. The longevity of crop seeds stored under ambient conditions. Seed Science Research 20: 1-12.
Nakamura, K. L., E. L. Cardozo Jr, C. M. Donaduzzi & I. Schuster. 2009. Genetic variation of phytochemical compounds in progenies of Ilex paraguariensis St. Crop Breeding and Applied Biotechnology 9: 116-123.
NBN (National Biodiversity Network). 2017. The NBN Gateway. Accessed on March 10, 2017. Available from: https://data.nbn.org.uk/imt/
Nehlig, A. 1999. Are we dependent upon coffee and caffeine? A review on human and animal data. Neuroscience and Biobehavioral Reviews 23: 563.
Nutt, D. J., L. A. King & L. D. Phillips. 2010. Drug harms in the UK: a multi-criteria decision analysis. Lancet 376: 1558-1565.
Nyman, U. & J. G. Bruhn. 1979. Papaver bracteatum--a summary of current knowledge. Journal of Medicinal Plant Research 35: 97-117.
Ohlsson, A., C. I. Abou-Chaar, S. Agurell, I. M. Nilsson, K. Olofsson & F. Sandberg. 1971. Cannabinoid constituents of male and female Cannabis sativa. Bulletin of Narcotics 23: 29-32.
Ojala, A. & A. Rousi. 1986. Interspecific hybridization in Papaver. 1. [F.sub.1] hybrids of P. somniferum with perennial species of sect. Oxytona. Annals Botanica Fennici 23: 298-303.
Okada, T., M. Mikage & S. Sekita. 2008. Molecular characterization of the phenylalanine ammonia-lyase from Ephedra sinica. Biological Pharmacy Bulletin 31: 2194-2199.
Opeke, L. K. 1984. Tropical tree crops. Spectrum Books, Ibadan, Nigeria.
Ou, D. W., M. L. Shen & Y. D. Luo. 1989. Effects of cocaine on the immune system of Balb/C mice. Clinical Immunology and lmmunopathology 52: 305-312.
Page, J. & M. Ware. 2015. Perspective: Close the knowledge gap. Nature 525: S9.
Paul, E. M., K. Capiau, M. Jacobs & J. M. Dunwell. 1995. A study of gene dispersal via pollen in Nicotiana tabacum using introduced genetic markers. Journal of Applied Ecology 32: 875-882.
Pertwee, R. G. 2006. Cannabinoid pharmacology: the first 66 years. British Journal of Pharmacology 147: S163-S171.
PIER. 2008. Institute of Pacific Islands Forestry Pacific Island Ecosystems at Risk (PIER). Plant Threats to Pacific ccosystems. Ilex paraguariensis. http://www.hcar.org/picr/wra/pacific/ilex_paraguariensishtmlwra.htm.
Pijlman, F. T. A., S. M. Rigter, J. Hoek, H. M. J. Goldschmidt & R. J. M. Niesink. 2005. Strong increase in total delta-THC in cannabis preparations sold in Dutch coffee shops. Addiction biology 10: 171-180.
Plowman, T. 1979. Botanical perspectives on Coca. Journal of Psychedelic Drugs 11:103-117.
Plowman, T. 1980. Botanical perspectives on Coca. Pp 99-105. In: F. R. Jcri (cd). Cocaine 1980, Proceedings of the inter-American seminar on medical and sociological aspects of Coca, Cocainc. Pacific Press, Lima.
Potter, G. R., M. J. Barratt, A. Malm, M. Bouchard, T. Blok, A. S. Christensen, T. Decorte, V. A. Frank, P. Hakkarainen, A. Klein & S. Lenton. 2015. Global patterns of domestic cannabis cultivation: Sample characteristics and patterns of growing across eleven countries. International Journal of Drug Policy 26: 226-237.
Prasifka, J. R., R. L. Hellmich, G. P. Dively & L. C. Lewis. 2005. Assessing the effects of pest management on non-target arthropods: The influence of plot size and isolation. Environmental Entomology 34: 1181-1192.
Priolll, R. H. G., P. Mazzafera, W. J. Siqueira, M. Moller, M. Imaculada Zucchi, L. C. S. Ramos, P. B. Gallo & C. A. Colombo. 2008. Caffcinc inheritance in interspecific hybrids of Coffea arabica x Coffea canephora (Gentianales, Rubiaceac). Genetics and Molecular Biology 31: 498-504.
ProtoWorld Flora Online. 2017. Proto World Flora Online. Accessed on March 10, 2017. Available at: http://proto. worldfloraonline.org/Homc.aspx?namcid=100016&dtlsVisible=1
Qazilbach, N. A. 1971. Pakistan Ephedra. Pharmacologische Weckblad 106: 345-349.
Qiao, G. & G. Zhang. 2002. Ephedraphis Hille Ris Lambers, a newly recorded genus from China (Homoptera:Aphididae). Acta Zootaxonomica Sinica 27: 544-547.
Raybould, A., D. Stacey, D. Vlachos, G. Graser, X. Li & R. Joseph. 2007. Non-target organism risk assessment of M1R604 maize expressing mCty3A for control of com rootworm. Journal of Applied Entomology 131: 391-399.
de Resende, M. D. V., J. A. Sturion, A. P. de Carvalho, R. M. Simeao & J. S. C. Fernandes. 2000. Avaliacao genetica de populacoes, progenies, individuos c clones de erva-mate no Parana. EMBRAPA Florest--Circular Tecnica. 43: 57-59.
Ren, N. & M. P. Timko. 2000. AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana spccies. Genome 44: 559-571.
Ricco R. A., M. L. Wagner & A. A. Gurni. 1995. Estudio comparativo dc flavonoides en especies austrosudamericanas del genero Ilex. Pp 243-250. In: H. Wingc, A. G. Ferreira. J. E. A. Mariath & L. C. Tarasconi (cds). Erva-Mate: Biologia c Cultura no Cone Sul. Editora da Univcrsidadc/UFRGS. Porto Alegre, Brazil.
Romeis, J., D. Bartsch, F. Bigler, M. P. Candolfi, M. M. C. Gielkens, S. E. Hartley, R. L. Hellmich, J. E. Huesing, P. C. Jepson, R. Layton, H. Quemada, A. Raybould, R. I. Rose, J. Schiemann, M. K.
Sears, A. M. Shelton, J. Sweet, Z. Vaituzis & J. D. Wolt. 2008. Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nature Biotechnology 26:203-208.
Rong, J., Z. Song, J. Su, H. Xia, B.-R. Lu & F. Wang. 2005. Low frequency of transgene flow from Bt/ CpTI rice to its nontransgenic counterparts planted at close spacing. New Phytologist 168: 559-566.
Ross, S. A., Z. Mehmedic, T. P. Murphy & M. A. Elsohly. 2000. GC-MS analysis of the total delta9-THC content of both drug- and fiber-type cannabis seeds. Journal of Analytical Toxicology 24: 715-717.
Rothschild, M. & J. W. Fairbairn. 1980. Ovipositing butterfly (Pieris brassicae L.) distinguishes between aqueous extracts of two strains of Cannabis sativa L. and THC and CBD. Nature 286: 56-59.
Rothschild, M., M. G. Rowan & J. W. Fairbairn. 1977. Storage of cannabinoids by Arctia caja and Zonocems elegans fed on chemically distinct strains of Cannabis sativa. Nature 266: 650-651.
Russel, T. A. 1955. The kola of Nigeria and the Cameroons. Tropical Agriculture (Trinidad) 32: 210-240.
Sagers, C. L., J. P. Londo, N. Bautista, E. H. Lee, L. S. Watrud & G. King. 2015. Benefits of transgenic insect resistance in Brassica hybrids under selection. Agronomy 5: 21-23.
Sagheer, M, K. Ali, M. ul-Hasan, A. Rashid, U. Sagheer & A. Alvi. 2013. Repellent and toxicological impact of acetone extracts of Nicotiana tabacum. Pegnum liermala, Saussurea costus and Salsola baryosma against red flour beetle, Tribolium castaneum (Herbst). Pakistan Journal of Zoology 45: 1735-1739.
Sands, D. C., E. J. Ford, R. V. Miller, B. K. Sally, M. K. McCarthy, T. W. Anderson, M. B. Weaver, C. T. Morgan, A. L. Pilgeram & L. C. Darlington. 1997. Characterization of a vascular wilt of Ervthroxylum coca caused by Fusarium oxysporum f. sp. erythroxyli forma specialis nova. Plant disease 81: 501-504.
Sano, H., Y. S. Kim & Y. E. Choi. 2013. Like cures like: caffeine immunizes plants against biotic stresses. Advances in Botanical Research 68: 273-300.
Sealy, J. R. 1958. A revision of the genus Camellia. Royal Horticultural Society, London. UK.
Sevcikova, M., I. Hrebickova, E. Macuchova & R. Slamberova. 2017. The influence of methamphetaminc on maternal behavior and development of the pups during the neonatal period. International Journal of Developmental Neuroscience 59: 37-46.
Siegmund, B., E. Leitner & W. Pfannhauser. 1999. Determination of the nicotine content of various edible nightshades (Solanaceac) and their products and estimation of the associated dietary nicotine intake. Journal of Agricultural and Food Chemistry 47: 3113-3120.
Siddiqui, A., S. Haq, S. Shaharyar & S. G. Haider. 1995. Morphine induces reproductive changes in female rats and their male offspring. Reproductive Toxicology 9: 143-151.
Silva, R. S., F. R. Ribeiro, O. S. Queiroz, 1. B. Santos, M. G. A. Oliveira, R. R. Pereira & M. C. Picanco. 2015. Trypsin protease inhibitor activity is not a good proxy for dcfencc against Oligonychus ilicis (Acari: Tctranychidae) in Coffea canephora (Gentianalcs: Rubiaceac). International Journal of Acarology 41: 189-194.
Sirikantaramas, S., S. Morimoto, Y. Shoyama, Y. Ishikawa, Y. Wada, Y. Shovama & F. Taura. 2004. The gene controlling marijuana psychoactivity: molecular cloning and heterologous expression of [DELTA]1- tctrahydrocannabinolic acid synthase from Cannabis sativa L. Journal of Biological Chemistry 279: 39767-39774.
Small, E. 1974. Morphological variation of achenes of Cannabis. Canadian Journal Botany 53: 978-987.
Small, E. 1984. Hybridization in the domesticated-weed-wild complex. Pp 195-210. In: W. F. Grant (cd). Plant Biosystematics. Academic Press Inc., Orlando, FL, USA.
Small, E. 2004. Narcotic plants as sources of medicinal, nutraccutical. and functional foods. Pp 11-67. In: F. F. Hou, H. -S. Lin, M. -H. Chou, and T. -W. Chang (cds). Proceedings of the International Symposium on the Development of Medicinal Plants, Hualien District Agricultural Research and Extension Station, Haulien, Taiwan. 24-25 Aug, 2004. http://www.hdarcs.gov.tw/htmlarca file/wcb articles/hdais/4487/021_2.pdf
Small, E. 2006. Culinary Herbs. 2nd edition. NRC Research Press. Ottawa.
Small, E. 2010. Blossoming treasures of biodiversity 33. Non-narcotic drug poppies--benefits for people and biodiversity. Biodiversity 11: 73-80.
Small, E. 2015. Evolution and classification of Cannabis sativa (marijuana, hemp) in relation to human utilization. Botanical Reviews 81: 189-294.
Small, E. 2016. Cannabis: a complete guide. Tayor & Francis/CRC Press, Boca Raton
Small, E. 2017. Classification of Cannabis sativa in relation to agricultural, biotechnological, medical and recreational utilization. Pp 1-62. In: S. Chandra, II. lata & M. A. ElSohly (cds). Cannabis sativa L.: Botany and Biotechnology. Springer-Verlag, Berlin.
Small, E. & T. Antle. 2003. A preliminary study of pollen dispersal in Cannabis sativa in relation to wind direction. Journal of Industrial Hemp 8: 37-50.
Small, E. & H. D. Beckstead. 1973. Common cannabinoid phenotypes in 350 stocks of Cannabis. Lloydia 36: 144-165.
Small, E. & P. M. Catling. 2001. Blossoming treasures of biodiversity: 3. Mate (Ilex paraguariensis)--better than Viagra, marijuana, and coffee? Biodiversity 2: 26-27.
Small, E. & P. M. Catling. 2006. Blossoming treasures of biodiversity: 22. Tobacco: another old star with a new act. Biodiversity 7: 47-54.
Small, E., T. Pocock & P. B. Cavers. 2003. The biology of Canadian weeds. 119. Cannabis sativa L. Canadian Journal of Plant Sciences 83: 217-237.
Smith, H. H. 1965. Inheritance of alkaloids in introgrcssivc hybrids of Nicotiana. American Naturalist 99: 73-79.
Snow, A. A., D. Pilson, L. H. Rieseberg, M. J. Paulsen, N. Pleskac, M. R. Reagon, D. E. Wolf & S. M. Seibo. 2003. A Bt transgene reduces herbivory and enhances fecundity in wild sunflowers. Ecological Applications 13: 279-286.
Snow, A. A., D. A. Andow, P. Gepts, E. M. Halierman, A. Power, J. M. Tiedje & L. L. Wolfenbarger. 2005. Genetically engineered organisms and the environment: current status and recommendations. Ecological Applications 15: 377-404.
Snow, A. A., T. M. Cullev, L. G. Campbell, S. G. Hegde & N. C. Ellstrand. 2010. Long-term persistence of crop alleles in weed populations. New Phytologist 186: 537-548.
Somorin, A. T. 1973. Spectrophotometric determination of caffeine in Nigerian kola nuts. Journal of Food Science 38: 911-912.
Spronk, D. B., J. H. P. van Wei, J. G. Ramaekers & R. J. Verkes. 2013. Characterizing the cognitive effects of cocaine: A comprehensive review. Neuroscience and Biobchavioral Reviews 37: 1838-1859.
State Herbarium of South Australia. 2017. cFlora of South Australia. Accessed March 10, 2017. Available at: http://www.flora.sa.gov.au
Stefano, G. B., A. Digenis, S. Spector, M. K. Leung, T. V. Bilfinger, M. H. Makman, B. Scharrer & N. N. Abumrad. 1993. Opiate-likc substances in an invertebrate, an opiate receptoron invertebrate and human immunocytes, and a role in immunosuppression. Proceedings of the National Academy of Sciences 90: 11099-11103.
Steppuhn, A., K. Gase, B. Krock, R. Halitsehke & I. T. Baldwin. 2004. Nicotine's defensive function in nature. PLoS Biology 2: c217.
Stoffelen, P. 1998. Coffea and Psilanthus in Tropical Africa: a systematic and palynological study, including a revision of the West and Central African Species. PhD Thesis. Katholicke Universiteit Leuven.
Sugiyama, A., C. M. Sano, K. Vazaki & H. Sano. 2016. Caffeine fostering of mycoparasitic fungi against phytopathogens. Plant Signaling and Behavior 11: c1113362.
SuZhen, N., S. Qin Fei & Y. Jic. 2011. Effect of different cutting on asexual propagation of feral tea tree. Acta Agriculturae Zhejiangensis 23: 905-909.
Suzuki, T. & G. R. Waller. 1987. Allelopathy due to purine alkaloids in tea seeds during germination. Plant and Soil 98: 131-136.
Taura, F., S. Sirikantaramas, Y. Shoyama, Y. Shoyama & S. Morinioto. 2007. Phytocannabinoids in Cannabis sativa: recent studies on biosynthctic enzymes. Chemistry and Biodiversity 4: 1649-1663.
Tachie-Obeng, E. & N. Brown. 2004. Kolanuts (Cola nitida and Cola acuminata). Pp 87-120. In: 1. E. Clark & T. C. H. Sunderland (cds). The Key Non-Timber Forest Products of Central Africa: State of the Knowledge. Technical Paper No. 122. May 2004. SD Publication Series Office of Sustainable Development Bureau for Africa. U.S. Agency for International Development, Ghana.
Takeda, Y. 1990. Cross compatibility of tea (Camellia sinensis) and its allied species in the genus Camellia. Japan Agricultural Research Quarterly 24: 111-116.
Tetenyi, P. 1977. Opium poppy (Papaver somniferum): botany and horticulture. Horticultural Reviews 19: 373-408.
Thomson, J. D., M. A. Dragulcasa & M. G. Tan. 2015. Flowers with caffeinated nectar receive more pollination. Arthropod-Plant Interactions 9: 1-7.
Turner, C. E., O. J. Bouwsma, S. Billets & M. A. Elsolily. 1980. Constituents of Cannabis sativa L. XVIIIElectron voltage selected ion monitoring study of cannabinoids. Biomedical Mass Spectrometry 7: 247-256.
UNGASS (United Nations General Assembly Special Session). 2016. International Drug Policy Consortium, April 19th--21st April 2016, New York, USA. http://idpc.net/theme/ungass-2016
U.S. Congress. 1993. Office of Technology Assessment. Alternative Coca Reduction Strategies in the Andean Region. OTA-F-556 Washington, DC: US Government Printing Office.
USDA, ARS, National Genetic Resources Program. 2007. Germplasm Resources Information Network (GRIN) [Online Database], Beltsville, Maryland: Natl. Germplasm Resources Laboratory. Available from: http://www.ars-grin.gov/cgibin/npgs/html/taxon.p1719756. Accessed Oct 31. 2017.
USDA, APHIS (Animal and Plant Health Inspection Service). 2008. Finding of no significant impact and decision notice [regarding risk of gene escape from a genetically engineered Nicotiana clone]. https://www.aphis.usda.gov/brs/aphisdocs/05_35403r ca.pdf
USDA-NRCS. 2017. The PLANTS Database (http://plants.usda.gov. 10 March 2017). National Plant Data Team, Greensboro, NC 27401-4901 USA.
USDA. 2016. Global Agricultural Trade System Online. http://apps.fas.usda.gov/GATS/default.aspx, Accessed November 11, 2016.
US Environmental Protection Agency (US-EPA). 2001. Biopesticide registration action document. Bacillus thuringiensis (Br) plant-incorporated protectants. 15 October 2001.
van Dam, N. M. & H. J. Bouwmeester. 2016. Metabolomics in the rhizosphcre: tapping into belowground chemical communication. Trends in Plant Science 21: 256-265.
van der Pijl, L. 1982. Principles of dispersal in higher plants. Springer-Verlag, Berlin.
Van der Werf H. M. G., E. W. J. M. Mathijssenm & A. J. Haverkort. 1996. The potential of hemp (Cannabis sativa L.) for sustainable fibre production: A crop physiological appraisal. Annals of Applied Biology 129: 109-123.
Van Wvk, B. E. & M. Wink. 2017. Medicinal plants of the world. Medicinal plants of the world., 2nd Ed.
Vigni, I. L. & M. R. Melati. 1999. Examples of seed dispersal by entomochory. Acta Botanica Gallica 146: 145-156.
Vincent, H., J. Wiersema, S. Kell, H. Fielder, S. Dobbie, N. P. Castaneda-Alvarez, L. Guarino, R. Eastwood, B. Leo & N. Maxted. 2013. A prioritized crop wild relative inventory to help underpin global food security. Biological Conservation 167: 265-275.
von Gernet, A. 1992. Hallucinogens and the origins of the lroquoian pipe/tobacco/smoking complex. Pp 171-185. In: C. F. Hayes, editor. Proceedings of the 1989 Smoking Pipe Conference. Rochester, NY: Rochester Museum of Science Centre.
Waffle, M., Y. Mekasha, M. Urge, G. Abebe & A. L. Goetsch. 2012. Effects of form of leftover khat (Catha edulis) on feed intake, digestion, and growth performance of Hararghe Highland goats. Small Ruminant Research 102: 1-6.
Wang, X., Z. He & C. Wei. 2017. A new cicada species of Psalmocharias Kirkaldy feeding on an Ephedra plant from China (Hemiptera: Cicadidac). Zootaxa 4290: 367-372.
Wang, J. H., J. Rizak, Y. M. Chen, L. Li, X. T. Hu & Y. Y. Ma. 2013. Interactive effects of morphine and dopaminergic compounds on spatial working memory in Rhesus monkeys. Neuroscience Bulletin 29: 3746.
Weiblen, G. D., J. P. Wenger, K. J. Craft, M. A. ELSohly, Z. Mehmedic, E. L. Treiber & M. D. Marks. 2015. Gene duplication and divergence affecting drug content in Cannabis sativa. New Phytologist 208: 1241-1250.
Wellnian, F. L. 1961. Coffee, botany, cultivation and utilization. London: Leonard Hill Books.
Wendel, J. F. & R. G. Percy. 1990. Allozyme diversity and introgression in the Galapagos Islands endemic Gossypium darwinii and its relationship to continental G. barbadense. Biochemical Systematics and Ecology 18: 517-528.
Wendt, S. N., V. A. Souza, M. Quoirin, A. M. Sebbenn, M. C. Mazza & J. A. Sturion. 2007. Caracterizacao genetica de procedencias e progenies de Ilex paraguariensis St. Hil. utilizando marcadores RAPD. Scientia Forestalls 73: 47-53.
White, L. M., S. F. Gardner, B. J. Gurley, M. A. Marx, P. L. Wang & M. Estes. 1997. Pharmacokinetics and cardiovascular effects of MaHuang (Ephedra sinica) in normotensive adults. Journal of Clinical Pharmacology 37: 116-122.
Whitney, K. D., R. A. Randell & L. H. Rieseberg. 2006. Adaptive introgression of herbivore resistance traits in the weedy sunflower Helianthus annuus. The American Naturalist 167:794-3807.
Whitney, K. D., J. R. Ahern, L. G. Campbell, L. P. Albert & M. S. King. 2010. Patterns ofhybridization in plants. Perspectives in Plant Ecology. Evolution, and Systematics 12: 175-182.
Williams, S. 2010. On islands, insularity, and opium poppies: Australia's secret pharmacy. Environmental Planning. Series D 28: 290-310.
Wink, M. 1988. Plant breeding: importance of plant secondary metabolites for protection against pathogens and herbivores. Theoretical and Applied Genetics 75: 225-233.
Winston, M. E., J. Hampton-Marcell, I. Zarraonaindia, S. M. Owens, C. S. Moreau, J. A. Gilbert, J. Hartsel, S. J. Kennedy & S. M. Gibbons. 2014. Understanding cultivar-specificity and soil determinants of the cannabis microbiomc. PloS One 9: e99641.
Winzer, T., M. Kern, A. J. King, T. R. Larson, R. I. Teodor, S. L. Donninger, Y. Li, A. A. Dowle, J. Cartwright, R. Bates & D. Ashford. 2015. Morphinan biosynthesis in opium poppy requires a P450oxidoreductase fusion protein. Science 349: 309-312.
Wright, G. A., D. D. Baker, M. J. Palmer, D. Stabler, J. A. Mustard, E. F. Power, A. M. Borland & P. C. Stevenson. 2013. Caffeine in floral nectar enhances a pollinator's memory of reward. Science 339: 1202-1204.
Wolfenbarger, L. L., S. E. Naranjo, J. G. Lundgren, R. J. Bitzer & L. S. Watrud. 2008. Si crop effects on functional guilds of non-target arthropods: a meta-analysis. PLoS One 3: c2118.
Wu, H., Z. Ma, M. M. Wang, A. L. Qin, J. H. Ran & X. Q. Wang. 2016. A high frequency of allopolyploid speciation in the gymnospcrmous genus Ephedra and its possible association with some biological and ecological features. Molecular Ecology 25: 1192-1210.
Wylie, S. J., C. Zhang, V. Long, M. J. Roossinck, S. H. Koh, M. G. K. Jones, S. Iqbal & H. Li. 2015. Differential responses to virus challenge of laboratory and wild accessions of Australian species of Nicotiana, and comparative analysis of RDR1 gene sequences. PloS One 10: c0121787.
Wynne-Edwards, K. E. 2001. Evolutionary biology of plant defenses against herbivory and their predictive implications for endocrine disruptor susceptibility in vertebrates. Environmental Health Perspectives 109: 443-448.
Yang, H., C. L. Wei, H. W. Liu, J. L. Wu, Z. G. Li, L. Zhang, J. B. Jian, Y. Y. Li, Y. L. Tai, J. Zhang & Z. Z. Zhang. 2016. Genetic divergence between Camellia sinensis and its wild relatives revealed via genome-wide SNPs from RAD sequencing. PLoS ONE 11: cO 151424.
Yang, Y., M. M. Lewis, A. M. Bello, E. Wasilewski, H. A. Clarke & L. P. Kotra. 2017. Cannabis sativa (hemp) seeds, A9-tctrahydrocannabinol, and potential overdose. Cannabis and Cannabinoid Research 2: 274-281.
Zaghloul, S. S., S. A. Nada & A. G. Radwan. 2003. Effect of khat on the rat thyroid gland. Toxicology 191: 47-48.
Zhang, Q. Y., M. Zhang & Y. Cao. 2012. Exposure to morphine affects the expression of endocannabinoid receptors and immune functions. Journal of Ncuroimmunology 247: 52-58.
Zhang, X., Z. Zhang & T. Stutze!. 2014. Ontogeny of the ovule and seed wing in Catha edulis (Vahl) Endl. (Celastraceae). Flora-Morphology, Distribution, Functional Ecology of Plants 209: 179-184.
Zhao, D. W., J. B. Yang, S. X. Yang, K. Kato & J. P. Luo. 2014. Gcnctic diversity and domestication origin of tea plant Camellia taliensis (Thcaccae) as revealed by microsatcllite markers. BMC Plant Biology 14: 14.
Zhu, Y.-P. 1998. Chinese Materia Medica: Chemistry, Pharmacology and Applications. Harwood Academic, Amsterdam, The Netherlands.
Ziegler, J., M. L. Diaz-Chavez, R. Kramell, C. Ammer & T. M. Kutchan. 2005. Comparative macro-array analysis of morphine containing Papaver somniferum and eight morphine-free Papaver species identifies an O-mcthyltransfcrasc involved in bcnzylisoquinolinc biosynthesis. Planta 222: 458-471.
Zong, N. & C. Wang. 2004. Induction of nicotine in tobacco by hcrbivory and its relation to glucose oxidase activity in the labial gland of three noctuid caterpillars. Chinese Science Bulletin 49: 1596-1601.
L. G. Campbell (1,3) * C. M. Blanchette (1) * E. Small (2)
(1) Department of Chemistry and Biology, Ryerson University. Toronto, Ontario M5B 2K3. Canada
(2) Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, Ontario KIA 0C6, Canada
(3) Author for Correspondence; e-mail: firstname.lastname@example.org Published online: 18 March 2019
Caption: Fig. 1 Pathways of gene flow in crop-feral Cannabis sativa mcta-populations. Most gene flow can occur by unidirectional pollen flow from fibre and seed hemp to feral populations. Seed dispersal from crop plants results in volunteers that can backcross with feral plants. Seed movement may start from a crop population to start volunteer populations and could result in the introgression of maternally inherited crop alleles whereas pollen movement can occur in both directions. Volunteers may be able to create feral populations
Caption: Fig. 2 The capacity for gene flow to result in the transfer genes from opium poppies (Papaver somniferum) to other spccies is influenced by A) the sexual compatibility of Papaver species and B) the overlap in geographie distributions of sexually compatible spccies. In this abstract representation of the geographic distribution of P. somniferum, there are two locations where gene flow among five sexually compatible species could occur. West Asia and Europe. Colour coding in B is described in A. Sexual compatibility described in: Goldblatt (1974): Malik et al. (1979); Nyman and Bruhn (1979); Kadercit (1986); Levy and Milo (1991). Geographic distributions described in Chitty et al. (2003)
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|Author:||Campbell, L.G.; Blanchette, C.M.; Small, E.|
|Publication:||The Botanical Review|
|Date:||Jun 1, 2019|
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