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Rickettsia felis as emergent global threat for humans.

Rickettsia felis is an emergent pathogen belonging to transitional group rickettsiae. First described in 1990, R. felis infections have been reported to occur worldwide in fleas, mammals, and humans. Because clinical signs of the illness are similar to those of murine typhus and other febrile illnesses such as dengue, the infection in humans is likely underestimated. R. felis has been found throughout the world in several types of ectoparasites; cat fleas appear to be the most common vectors. R. felis infection should be considered an emergent threat to human health.

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Rickettsia felis is a member of the genus Rickettsia, which comprises intracellular pathogens that produce infections commonly called rickettsioses. Although the genus has no recognized subspecies, rickettsiae have traditionally been subdivided into 2 groups: the spotted fever group (SFG) and the typhus group. Infections produced by these 2 groups are clinically indistinguishable; however, groups can be differentiated by outer membrane protein OmpA (absent in the typhus group) and by vector. SFG members are transmitted by ticks; typhus group members, by fleas and lice (1,2). More recently, Gillespie et al. (3) added to this classification by designating the transitional group of rickettsiae and describing an ancestral group of rickettsiae.

In 1990, Adams et al. described a rickettsia-like organism, which resembled R. typhi, in the cytoplasm of midgut cells of a colony of cat fleas (1). The new rickettsia received the initial name of ELB agent after the company from which the fleas were obtained (El Labs, Soquel, CA, USA) (4). The first observations, such as reactivity with antibodies to R. typhi (1), the type of vector in which it was first discovered (1), and the apparent absence of OmpA (5), suggested that the new organism belonged to the typhus group of rickettsiae (4).

The molecular characterization of the organism described by Adams and reported by Bouyer et al. in 2001 provided sufficient evidence to support the designation of R. felis as a member of the SFG (6), and in 2002, La Scola et al. provided further characterization (7). One noticeable characteristic is the temperature-dependent growth of the bacterium, which requires incubation temperatures of 28[degrees]-32[degrees]C for optimal growth. However, the most striking characteristic of the novel rickettsia was the plasmid DNA in its genome (8).

World Distribution in Potential Host Vectors

Soon after the initial description of the typhus-like rickettsia, Williams et al. (9) reported that cat fleas collected from opossums in an urban setting in California were infected with the novel rickettsia, but no organism was detected in the tissues of the opossums. Since this report, this organism has been described in infected vectors from 20 countries on 5 continents (9). Not until 2002 did interest in R. felis increase, when the United States (9), Brazil (10), Mexico (11), and Spain (12) were among the first countries to describe cat fleas (Ctenocephalides felis) infected with R. felis. During the following 5 years, 28 additional reports appeared from all over the world (Table 1). These reports describe new potential vectors being infected with the emergent rickettsia, including the following: fleas, such as C. canis (13 15), Anomiopsyllus nudata (16), Archaeopsylla erinacei (15,17), Ctenophthalmus sp. (17), and Xenopsylla cheopis (18); ticks, Haemaphysalis flava (19), Rhipicephalus sanguineus (20), and Ixodes ovatus (19); and mites from South Korea (21) (Table 1). Despite the large number of potential vectors reported, the only vector currently recognized is C. felis because it has been demonstrated that this flea is able to maintain a stable infected progeny through transovarial transmission (4). In addition, production of antibody to R. felis has been noted in animals after they have been exposed to infected cat fleas (9). Other evidence to be considered is the fact that 68.8% of the reports state that the cat flea is the most recurrent vector in which R. felis has been detected. These data further support the wide distribution of rickettsiae because they correlate with the worldwide distribution of C. felis; this distribution represents a threat to the human population because of lack of host specificity of the cat flea.

R. felis infection is diagnosed by PCR amplification of targeted genes. The genes most commonly amplified by researchers are gltA and ompB; followed by the 17kDa gene. Also, 25% of published articles report that R. felis was detected by amplifying >2 genes, and all report that amplicons were confirmed as R. felis by sequencing. The animal hosts from which the infected ectoparasites were recovered represent a diversity of mammals (Table 1), which included 9 different naturally infested animal species. However, in 16 of 33 articles, ectoparasites were recovered from dogs. Other hosts for ectoparasites were cats (in 13 of 33 reports); rodents (5 of 33 reports); opossums and hedgehogs (2 reports each); and horses, sheep, goats, gerbils, and monkeys (1 report for each animal species).

In summary, the presence of R. felis in a diverse range of invertebrate and mammalian hosts represents a high potential risk for public health and the need for further studies to establish the role of ectoparasites other than C. felis as potential vectors. To date, whether any vertebrate may serve as the reservoir of this emergent pathogen has not been determined. However, preliminary data from our laboratory suggest that opossums are the most likely candidates.

World Distribution of Human Cases

In 1994, the first human case of infection with the new cat flea rickettsia was reported in the United States (2). This became the first evidence of R. felis' potential as a human pathogen. R. felis infection had a similar clinical manifestation as murine typhus (including high fever [39[degrees]-40[degrees]C], myalgia, and rash). Although the initial idea was that the murine typhus--like rickettsia had a transmission cycle involving cat fleas and opossums (2,5,9), no viable R. felis has yet been isolated from a vertebrate host.

Three more cases of R. felis infection were reported from southeastern Mexico in 2000. The patients had had contact with fleas or animals known to carry fleas. The clinical manifestations were those of a typical rickettsiosis: all patients had fever and myalgia; but the skin lesions, instead of a rash, were similar to those described for rickettsialpox. In addition, for 3 patients, central nervous system involvement developed, manifested as photophobia, hearing loss, and signs of meningitis (33).

As occurred with the fast-growing reports of the worldwide detection of R. felis in arthropod hosts, the reports of human cases of R. felis infection increased rapidly in the following years (Table 2). But, in contrast, only 11 articles reported human infection by R. felis compared with 32 that reported ectoparasite infection with the new rickettsia. Nevertheless, these findings indicate that an effective surveillance system is urgently needed to distinguish R. felis rickettsiosis from other rickettsial infections such as murine typhus and Rocky Mountain spotted fever, and from other febrile illnesses such as dengue. Although PCR is still a method of choice for many laboratories, its high cost prevents many from using the technique, particularly in developing countries. Important advances have been achieved in diagnostics, such as the recent establishment of a stable culture of R. felis in cell lines that allows its use as antigen in serologic assays differentiating the cat flea rickettsia from others. Use of this culture in the immunofluorecent assay has enabled detection of additional human cases (38).

The first autochthonous human case in Europe was reported in 2002, which demonstrated that R. felis has a potential widespread distribution and is not confined to the Americas. It also confirmed the risk for human disease anywhere in the world. After the first report in Europe of a human infection of R. felis, other human cases have appeared in other countries around the world, including Thailand (36), Tunisia (38), Laos (39), and Spain (40); additional cases have been reported in Mexico and Brazil (34). All the data support the conclusion that the incidence of R. felis rickettsiosis and the simultaneous worldwide distribution of the flea vector plausibly explain its endemicity.

At present, the involvement of domestic animals (e.g., dogs and cats) or wild animals coexisting in urban areas (e.g., opossums) maintains R. felis infection in nature. C. felis fleas serve as the main reservoir and likely have a central role in transmission of human illness.

Conclusions

R. felis is an emergent rickettsial pathogen with a worldwide distribution in mammals, humans, and ectoparasites. The clinical manifestations of R. felis infections resemble those of murine typhus and dengue, which makes them difficult to diagnose without an appropriate laboratory test. For this reason, infections due to this emergent pathogen are likely underestimated and misdiagnosed. Although R. felis may require only fleas for its maintenance in nature, we still do not know the role of animals in the life cycle of flea-borne spotted fever rickettsia. In addition, flea-borne spotted fever should be considered in the differential diagnosis of infectious diseases. Further research should be conducted to determine the actual incidence of R. felis infection in humans, the spectrum of clinical signs and symptoms, and the severity of this infection and also to assess the impact on public health.

DOI: 10.3201/eid1407.071656

Acknowledgment

We thank Patricia Croquet-Valdes for her comments and helpful advice.

This research was supported by grants from the CONACyT (44064-M) to J. E. Z.-V.

Mr. Perez-Osorio is currently professor of microbiology at the Autonomous University of Yucatan. His research interests focus on rickettsial diseases.

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Address for correspondence: Jorge E. Zavala-Castro, Facultad de Medicina, Unidad Interinstitucional de Investigacidn Clinica y Epidemioldgica, Universidad Autdnoma de Yucatan, Avenida Itzaes No. 498 x 59 y 59A Centro, CP 97000, Merida, Yucatan, Mexico; email: zcastro@uady.mx

Carlos E. Perez-Osorio, * Jorge E. Zavala-Velazquez, * Juan Jose Arias Lebn, * and Jorge E. Zavala-Castro *

* Universidad Autonoma de Yucatan, Merida, Mexico
Table 1. Potential vectors infected with Rickettsia felis reported
worldwide, 1992-2007 *

Year Source of DNA sample Animal
 ([dagger])

1992 Ctenocephalides felis Opossum
2002 C. felis Cats and dogs
2002 C. felis Dogs
2002 C.felis Cats and dogs
2003 Haemophysalis flava, H.
 kitaokai, and Ixodes ovatus Unknown (flagging)
2003 C. felis Cats
2003 C. felis Cats and dogs
2004 C. felis Dogs
2005 Anomiopsyllusnudata Wild rodents
2005 C. felis Cats and dogs
2005 C. felis Monkey
2006 C. felis and C. canis Dogs
2006 C. felis and C. canis Cats and dogs
2006 Archaeopsylla erinacei and C. canis Hedgehog and rodents
2006 A. erinacei and Ctenophtalmus sp. Rodents and hedgehog
2006 Xenopsylla cheopis Rodents
 ([double dagger])
2006 C. felis, Rhipicephalus sanguineus,
 and Amblyommma cajennense Dogs and horse
2006 Unknown flea Gerbil
2006 C. felis Cats and dogs
2006 C. felis Cats
2006 C. felis Rodents
2007 Mites Wild rodents
2007 C. felis Cats
2007 C. felis Cats

Year Country Reference

1992 USA (9)
2002 Brazil (10)
2002 Mexico (11)
2002 Spain (12)
2003
 Japan (19)
2003 France (22)
2003 UK (23)
2004 Peru (24)
2005 USA (16)
2005 New Zealand (25)
2005 Gabon (26)
2006 Brazil (13)
2006 Uruguay (14)
2006 Algeria (15)
2006 Portugal (17)
2006
 Indonesia (18)
2006
 Brazil (20)
2006 Afghanistan (27)
2006 Australia (28)
2006 Israel (29)
2006 Cyprus (30)
2007 South Korea (21)
2007 USA (31)
2007 Chile (32)

* PCR was used to detect R. felis infection with 1 noted exception.

([dagger]) Animal host of potential vectors.

([double dagger]) Quantitative PCR.

Table 2. Human cases of Rickettsis felis infection reported
worldwide, 1994-2006 *

Year No. cases Method

1994 1 PCR
2000, 2006 5 PCR
2001, 2006 3 PCR
2002 2 PCR/serology
2003 1 Serology (seroconversion)
2005 3 Serology (Western blot)
2006 8 Serology (IFAT/Western blot)
2006 1 Serology (seroconversion)
2006 33 Serology (IFAT)
Total 68

Year Country Reference

1994 USA (2)
2000, 2006 Mexico (33)
2001, 2006 Brazil (34)
2002 Germany (35)
2003 Thailand (36)
2005 South Korea (37)
2006 Tunisia (38)
2006 Laos (39)
2006 Spain (40)
Total

* IFAT, indirect fluorescent antibody test.
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Title Annotation:SYNOPSIS
Author:Perez-Osorio, Carlos E.; Zavala-Velazquez, Jorge E.; Leon, Juan Jose Arias; Zavala-Castro, Jorge E.
Publication:Emerging Infectious Diseases
Geographic Code:1MEX
Date:Jul 1, 2008
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