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Reproductive biology of Sodhiana iranica (Brachyura: Gecarcinucidae) from Southern Iran.

There are more than 6 800 species of brachyuran crabs distributed in all over the world. Of all species, over one-fifth are true freshwater crabs. Primary freshwater crabs have been introduced as land-locked organisms (Yeo et al., 2008; Cumberlidge, et al., 2009), as they are able to complete their life cycle independently of the marine environment. They are one of the most ecologically important macro-invertebrate groups (Yeo et al., 2008) which have the most number of species compared to the other decapod crustacean groups (Ng, Guinot, & Davie, 2008) in inland waters worldwide.

In general, the freshwater crab fauna is poorly known in Iran. The gecarcinucid Sodhiana rokitanskyi (Pretzmann, 1971), and its taxonomy has been widely discussed by Kamrani, Ng, Mirzadeh, and Nakhodai (2009). The type locality of S. rokitanskyi occurs near a hot spring in Geno, Southern Iran (Kamrani, Ng, Mirzadeh, & Nakhodai, 2009); this area was located about 200 km to the East of Bastak, Iran. Recent studies introduced more species of freshwater crabs such as Potamon bilobatum (Brandis, Storch, & Turkay, 2000) from Northern Iran (Potamidae, cf.; Nasrollahzadeh, Noveirian, & Souto hian, 2011) and Potamon ilam (Keikhosravi & Schubart, 2014) from Western Iran (Potamidae, cf; Keikhosravi, & Schubart 2014). All rivers populated by P ilam rise on the Western most slopes of the Zagros mountains in Western Iran; and this work increased to 22 the number of species in the genus Potamon.

The gecarcinucid fresh water crab Sodhiana iranica has been recently reported from Bastak (the same type of locality as S. rokitanskyi), Southern Iran for the first time as a new species. (Sharifian, Kamrani, & Sharifian, 2014). The species inhabits a freshwater springs located in a semi-mountainous area in Iran (Eellod Area, 431 m.a.s.l.), covered by dense stands of common reeds and salt cedar trees in the periphery, with mats of green algae on the bottom (Sharifian, Kamrani, & Sharifian, 2014). S. iranica represents the fourth species of Sodhicma, and is the second one known from Iran. Recently, some information about oogenesis and ovarian development of S. iranica has been discussed (Sharifian, Kamrani, Safaie, & Sharifian 2015). Histologically, the female germ cells were classified into seven different stages, and four ovarian developmental stages were found for females, based on the number and types of oocytes present in each stage. Besides, in a previous study, S. iranica morphometry variations have been reported and the authors determined that the growth of this crab is isometric (Sharifian, & Kamrani, 2015).

Reproductive biology plays a critical role in population dynamics and life history strategies of crustaceans. Reproductive biology of brachyuran crabs is extremely variable for different species in terms of egg's production and offspring's survival (Lopez-Greco, Hernandez, Bolanos, Rodriguez, & Hernandez, 2000). Reproductive biology studies provide basic information for stock management which is necessary to establish plans directed for sustainable management of living crab resources. The determination of breeding periods is controlled by a complex interaction of endogenous and exogenous factors, allowing both intra and interspecific variations, dealing with the duration of the reproductive season (Sastry, 1983).

Although numerous reports on reproductive biology of marine, intertidal and estuarine crabs are available (Kumar, Xiao, Venema, & Hooper, 2003; Lestang, Hall, & Potter, 2003; Sallam, 2005; Oh, Kim, Jeong, Suh, & Cho, 2006; Hemni, & Koga 2009; Omolara, 2010; Sahoo, Panda, & Guru, 2011), limited works are available on the population and reproductive biology of freshwater, semiterrestrial or land crabs (Mansur, & Hebling, 2002; Liu, & Jeng, 2005; Hartnoll, Broderick, Godley, & Saunders, 2009; Hartnoll et al., 2010; Patlire, & Mina, 2010; Devi, & Smija, 2013). In this study, we investigated the reproductive aspects in the recently described crab S. iranica, to contribute with new information to assess the reproductive cycle of this species for optimum management.

MATERIAL AND METHODS

The sampling site was located in the freshwater springs of the Eelood area in Honnozgan province (27[grados]13' N - 54[grados]40' E), Southern Iran (Fig. 1). This region has a subtropical climate, with distinct spring and summer seasons. On an annual average, the study area lias a pH of 8.09; salinity of 4 %, temperature ranging from 25 to 30 [grados]C, sediment Total Organic Matter (TOM) of 4.6 % with a composition of 50 % sand, 34 % silt, and 16 % clay.

Few specimens of crabs were collected every two months from April (2012) to April (2013), to minimize the sampling pressure impact on the population. Specimens were collected by three people (by hand) during the daytime and over an approximate period of four hours from the freshwater springs of the Eelood area. Samples were placed inside iced plastic buckets and they were directly transported to the laboratory of Honnozgan University, at a distance of 235 km from the sampling site, Iran.

In the laboratory, crabs were identified and examined for sex identification, and female crabs were separated from the rest of the samples. The carapace width (CW) was measured using vernier caliper ([+ o -] 0.05 mm accuracy) following Ng (1988). The total body weight (TW) was measured using a standard electronic balance (LA 310 S model, 0.0001 g accuracy). In order to measure the Gonado-somatic index (GSI) and Hepato-Somatic Index (HSI), we removed the carapace, and weighed the gonads and hepatopancreas.

The gonado-somatic index (GSI) was calculated according to the following equation (Giese, 1966):

GSI = GW (g)/TW (g) x 100;

where g = grams, GW is gonad weight and TW is total body weight.

The hepato-somatic index (HIS) was calculated using the following equation (Clarke, 1977):

HIS = HW (g)/TW-GW (g) x 100;

where g= grains, HW is hepatopancreas weight, TW is total body weight, and GW is gonad weight.

The oocytes diameter was assessed by measuring the diameter of 40 oocytes found in each adult female ovary, and a mean was obtained per month. The information obtained from the ovaries of five adult females per month was pooled, for a total sample size of 200 oocytes diameter per month. The frequency of the ovarian maturation stages found was used as an index to determine the reproductive cycle in female crabs of S. iranica (Biswas, 1993). The maturation stages were divided into four stages (Sharifian, Kamrani, Safaie, & Sharifian, 2015).

The mean carapace width (CW) of males and females was compared using the Student t-test. A Chi-squared test ([chi square]) was performed to detect significant deviations from a 1:1 sex ratio. Mann-Whitney U tests were applied to test differences among the carapace width (CW) of mature and immature females. Bimonthly mean oocyte diameters were compared using ANOVAs followed by post hoc Tukey's HSD. Kruskal-Wallis nonparametric test was used to determine the differences in GSI and HSI between different months. The relationship between GSI (%) and HSI (%) was studied using regression analysis and correlation coefficient (Zar, 1996).

RESULTS

A total of 158 crabs were collected during the study period: 89 males (56.3 %), and 69 females (43.6 %). The overall sex ratio (1:0.78) was not significantly different from the expected 1:1 proportion (Chi-square test (x2); p > 0.05). The females carapace width (CW) ranged from 12.24 to 34.52 mm (24.26 [+ o -] 0.79) (Table 1), and for males from 10.26 to 36.50 mm (23.07 [+ o -] 0.80). A comparison of mean CW in both sexes showed no significant differences (t-test; p > 0.05), and the same resulted between immature and mature females (Mann-Whitney U test; p > 0.05).

The relative bimonthly frequencies of the four maturation stages found were obtained for S. iranica females (Fig. 2). Spent females were mainly present from April to August (2012), with some spent females observed in April (2013). During the same months, stage II specimens were also present, with a slightly lesser extent. Stage III started in August (2012) and progressed rapidly, reaching a peak in October (2012). Maturation and spawning occurred from December (2012) to April (2013), when the frequency of stage IV specimens had the highest values. This stage peaked in February (2013), and showed signs of decline later in April (2013). Gametogenesis appeared to be continuous throughout the spawning period, as evident from the basal frequencies of stage III specimens during this period. The reproduction of S. iranica from the Eelood freshwater spring followed a single seasonal cycle, with the peak stage III in October (2012) and spawning occurring between December (2012) and April (2013). A low percentage, of females carrying either eggs or young ones, was observed from all samples.

The bimonthly fluctuation of oocytes diameter is given in figure 3. The mean of oocyte diameters varied significantly between different months (ANOVA; F = 3.86; p < 0.05). The most significant increase in the oocyte mean diameter occurred between August (2012) and February (2013) (from 189.54 [+ o -] 16.71 to 466.05 [+ o -] 67.80 [micro]m, respectively) (ANOVA; F = 3.52; p < 0.05). This pattern coincided with ovarian maturation stages and subsequent spawning during this period.

Kruskal-Wallis tests showed no significant differences in GSI values between different months (Kruskal-Wallis test; df = 6; p > 0.05). The Gonado-somatic indexes (GSI) reached an annual peak in February (2013), and a drop was found in the index value for April (2013), indicating a single annual spawning event (Fig. 4). The Gonado-somatic indexes (GSI) values reached their annual minimum from June through October, after which the buildup of GSI was observed. The GSI values in April (2013) were similar to those of April (2012).

A significant difference in Hepato-somatic indexes (HSI) was observed between different months (Kruskal-Wallis test; df = 6; p < 0.05). The HSI (%) was observed lowest in June (2012), whereas a peak of hepatopancreas development was observed in April (2012) and February (2013), respectively (Fig. 5). The regression analysis showed that there is a weak correlation between GSI and HSI (%) (F = 0.41; p > 0.05, and R2 = 0.307). Macroscopic observation of the hepatopancreas resulted in the classification of four colors during the ovarian cycle. These colors were dark brown, dark cream, bright yellow, and dark yellow color.

DISCUSSION

This is a species found in local streams but is an important resource for local people. In terms of carapace width, any significant differences were observed between the sexes of S. iranica. Sex dimorphism in size is especially marked in aquatic brachyurans than in semiterrestrial and terrestrial ones (Pinheiro & Fransozo, 1999). In this study, the overall sex ratio for S. iranica was not significantly different from the expected ratio (1:1). The sex ratio was found the same as in the freshwater crab Sinopotamon yangtsekiense (Tao, Wei, & Nan-shan, 1994) and the crab Uca arcuata (Yamaguchi & Henmi, 2008).

Regarding to GSI, hepato-somatic index (HSI), and the mean of oocyte diameter in S. iranica which showed a rising trend during spawning period December (2012) to April (2013), with a drop in April (2013); this behavior suggested that the crab S. iranica is a single seasonal breeder. Seasonal breeding pattern has been also reported for Travancoriana schirnerae (Devi & Smija, 2013), Sodhiana rokitanskyi (Nakhodai, Kamrani, & Mirzadeh, 2013), Sartoriana spinigera (Rahman, Rhaman, Ahmed, Mollah, & Hossain, 2008), Sinopotamon yangtsekiense (Tao, Wei, & Nan-shan, 1994), and Potamon koolooense (Joshi & Khana, 1982). Like S. iranica, some gecarcinucid crabs have a short breeding season (Devi and Smija 2013).

The reproduction in tropical areas occur year round because environmental favorable conditions (Mouton & Felder, 1995), whereas the breeding is expected to be restricted to certain months in subtropical areas (Pillay & Nair, 1973). Nonetheless, exceptions to this hypothesis have been reported (Litulo, 2004).

Food availability is an important factor which triggers maturation and spawning (Wenner, 1977; Litulo, 2004). Regarding the breeding of S. iranica which was restricted to rainy and cold months, this suggests that ovarian maturation of S. iranica could be triggered by nutrient accumulation. Moreover, other contributing factors may be associated with the breeding activity such as temperature, salinity, oxygen, and photoperiod (Costa, & Negreiros-Fransozo, 2003; Mantelatto, Faria, & Garcia, 2003). Generally, female crabs prefer warmer water where they produce eggs more rapidly. Egg development time was considerably shortened by warm water (Fusaro, 1980). Food availability also has a direct effect on egg production (Wenner, 1977). Litulo (2005) suggests seasonal breeding patterns of crabs in subtropical areas may be based on the population's evolutionary histories or abiotic and biotic factors.

Like S. iranica, observations for low percentage of ovigerous females were seen by Devi and Smija (2013) for the freshwater crab Travancoriana schirnerae from India, and by Mansur and Hebling (2002) in the neotropical freshwater crab Sylviocarcinus australis from Brazil. The reason for low percentage of ovigerous females may be attributed to the behavior of these female crabs, as they remain in their burrows during the entire incubation period, and foraging decreases when compared to other activities of this particular life phase (Mansur & Hebling, 2002).

There is a relationship between the hepatopancreas and ovary physiology in crustaceans. The carbon and lipid concentrations of hepatopancreas decrease during the ovarian maturation and subsequently, the concentrations of carbon and lipid in ovaries increase during this period (Felder & Hasek, 2005). Regarding macroscopic observations, the S. iranica ovary increased in size and color during the ovarian maturation. The gradual increase in the size of the ovarian cells has been attributed to the deposit of lipid in the ovaries during vitellogenesis (Revathi, Iyapparaj, Munuswamy, & Krishnan, 2012; Castiglioni, & NegreirosFransozo, 2006; Gregati, Fransozo, Lopez-Greco, & Negreiros-Fransozo, 2010). The color changes observed in the hepatopancreas can be attributed to changes in its contents as previously reported (water, carbon, nitrogen, among others) (Felder, & Hasek, 2005).

The information of reproductive biology of aquatic living resources, including GSI, ovarian development and reproductive season, is of priority for their optimum management and conservation. In this study we found that S. iranica is a single seasonal breeder. The information gathered from this study may be used as a baseline data for more comprehensive research in the future. An understanding of breeding period may also help protect females with eggs during the spawning season as part of the population and policy making.

Received 15-II-2016. Corrected 25-VIII-2016. Accepted 26-IX-2016.

ACKNOWLEDGMENTS

We are grateful to Hanieh Saeedi (Federal University of Espirito Santo) for her kind assistance in editing this paper. The authors also would like to acknowledge Aref Ghafori, Mansour Nami and Nima Nami for their field assistance in the Eelood region, Bastak, Iran.

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Sana Sharifian (1), Ehsan Kamrani (2), Mohsen Dehghani (1)

(1.) Department of Marine Biology, University of Hormozgan, P.O. Box 3995, Bandar Abbas, Iran; sharifian.phd@hormozgan.ac.ir, Mohsen.dehghani64@gmail.com

(2.) Department of Fisheries, University of Hormozgan, P.O. Box 3995, Bandar Abbas, Iran; Ezas47@gmail.com

Leyenda: Fig. 1. Sampling locations in Eelood Area, Hormozgan Province, Southern Iran.

Leyenda: Fig. 2. Sodhiana iranica Sharifian, Kamrani, & Sharifian, 2014. Frequency of maturation stages in adult females from Eelood Area, Hormozgan Province, Southern Iran. April 2012-April-2013.

Leyenda: Fig. 3. Sodhiana iranica Sharifian, Kamrani, & Sharifian, 2014. The bimonthly fluctuation of oocytes diameter at Eelood Area, Hormozgan Province, Southern Iran. Error bars [+ o -] standard error (SE).

Leyenda: Fig. 4. Sodhiana iranica Sharifian, Kamrani, & Sharifian, 2014. Temporal trends of the gonadosomatic index at Eelood Area, Hormozgan Province, Southern Iran. Error bars [+ o -] standard error (SE). April 2012-April-2013.

Leyenda: Fig. 5. Sodhiana iranica Sharifian, Kamrani, & Sharifian, 2014. Temporal trends of the hepatosomatic index at Eelood Area, Hormozgan Province, Southern Iran. Error bars [+ o -] standard error (SE). April 2012-April-2013.
TABLE 1

Sodhiana iranica carapace width (CW) comparison of males and females
from Eelood Area, Hormozgan Province, Southern Iran

          Sex                 N    Mean    S. E.   Max     Min
                                   (mm)

CW (mm)   Male                89   23.07   0.80    36.50   10.26
          Female (Total)      69   24.26   0.79    34.52   12.24
          Female (immature)   20   18.87   0.49    28.32   12.24
          Female (mature)     49   30.87   0.42    34.52   29.43

(N = sample number; S.E.= standard error; Max= Maximum; Min= Minimum).
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Author:Sharifian, Sana; Kamrani, Ehsan; Dehghani, Mohsen
Publication:Revista de Biologia Tropical
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Date:Mar 1, 2017
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