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1. Which of the following are members of the genus Bordetella?

A. B.pertussis, B.parapertussis, B. avium,

B. alcaligenes

B. B. pertussis, B. bronchiseptica, B. holmesii, B. trematum

C. B petrii, B. ansorpii, B. hinzii, B. xylosoxidans

D. B. parapertussis, B. trematum, B. alcaligenes, B. hinzii

2. Which of the following are the expected biochemical reactions for B. trematum?

A. Catalase positive, Indole negative, Oxidase negative

B. Catalase negative, Indole negative, Oxidase negative

C. Catalase positive, Indole positive, Oxidase positive

D. Catalase negative, indole positive, Oxidase negative

3. From what primary media will B. trematum grow?

A. 5% Sheep Blood agar, Chocolate agar

B. 5% Sheep Blood agar, MacConkey agar, 5% Sheep Blood with PEA

C. 5% Sheep Blood agar, Chocolate agar, 5% Sheep Blood with PEA

D. 5% Sheep Blood agar, Chocolate agar, MacConkey agar

4. What is the Gram stain morphology of B. trematum?

A. Large Gram negative bacillus

B. Large Gram positive bacillus

C. Small Gram negative bacillus

D. Small Gram positive bacillus

5. Which of the following techniques are most useful in the identification of B. trematum?

A. Vitek 2 System and biochemical testing

B. MALDI-TOF, biochemical testing, and 16S ribosomal RNA gene sequencing

C. Vitek 2 System and MALDI-TOF

D. MALDI-TOF and biochemical testing

6. Which two species of the genus Bordetella constitute the majority of human infections, causing upper respiratory tract disease?

A. B. pertussis, B. bronchiseptica

B. B. pertussis, B. trematum

C. B. parapertussis, B. avium

D. B. pertussis, B. parapertussis

7. What is the most useful approach in identifying rarely encountered organisms, such as B. trematum?

A. Gram stain and biochemical testing

B. Gram stain, biochemical testing and MALDI-TOF

C. 16S ribosomal RNA gene sequencing

D. Gram stain, biochemical testing, MALDI-TOF and 16S ribosomal RNA gene sequencing

8. Which diagnostic methodology is LEAST likely to result in an accurate species level identification of a Bordetella sp. isolate?

A. 16S ribosomal RNA gene sequencing

B. Biochemical testing

C. Species specific primer amplification by PCR


9. When B. trematum is isolated in the clinical laboratory it is ...

A. Usually considered clinically significant

B. Always considered clinically significant

C. Never considered clinically significant

D. Of uncertain clinical significance

10. B. trematum isolated from which body site is MOST likely to be clinically significant?

A. Fluid aspirated from the outer ear canal

B. Swab of a chronic leg wound in the context of greater than 5 additional colony morphologies by culture

C. Blood culture

D. Fresh tissue excised from a chronic leg wound in the context of greater than 5 additional colony morphologies by culture
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Title Annotation:Article 447 1 Clock Hour
Publication:Journal of Continuing Education Topics & Issues
Date:Jan 1, 2018
Previous Article:Case Thirty-Two: An Unusual Gram-Negative Bacillus Recovered from a Pediatric Patient with Chronic Otitis Media.
Next Article:Three Rapid Screening Tests for Cryptococcus Neoformans in the Laboratory.

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