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Quantitative analyses of parasite load in the cardinal tetra (Paracherodon axelrodii), along the period of capture and storage.

Introduction

Parasite studies are one of the most important studies for the activity of fish husbandry. It is known that in infected fish, ecto or endo parasites can cause much harm to the hatchery activity, such as malnourishment, slow growth, and eventual death [1]. A fish with parasites suffers changes in its behaviour, making it easy to diagnose a sick fish without autopsy. For example, a sick fish' coloration changes and becomes darker, sick fish swim closer to the water surface with reduced velocity, and they eat less. Hoare et al. [2] proved that according to their parasite load they are segregated into different shoals.

In edible fish, parasites do not matter since when they reach ideal consumable size consumption occurs. On the other hand, upon dealing with tropical fish for aquariums that have to be kept alive in order to be sold, parasites become a bigger issue. The vast majority of parasites can be treated with manufactured drugs, for example, Malaquite green, Metilene blue, acryflavin, formalin, and tetracycline [1,3]. Besides using drugs, a couple prophylactic measures should be taken to guarantee the health of the tank or aquarium. These measures are: maintaining the water clean, using clean instruments to handle fish, removal of food left overs and faecal matter. Most importantly, the place where the fish is lodged must have a good filtration system and these filter systems have to be maintained constantly cleaned [1].

It is important to mention that the improper handling of the fishes can cause great harm to their health. Transporting fish to different locations roughly can lower the efficiency of their immune systems, making them more susceptible to diseases. For example, catfish that have a large bony spine on their lateral fins can pierce one another while being carried on recipients with little water and abrupt movements and these piercing can open way for opportunistic bacterial infections that may lead to death.

Most of the research dealing with Amazonian fish parasitology concentrates on the diagnosis of these parasitic fauna and most fieldwork concentrates on the parasites of "Tambaqui" (Colossoma macropomum) [4,5]. Very little is known about which parasites infect smaller fish, which, due to their size, are more susceptible to infections. For example, Tavarez et al. [6] made an important study establishing which parasites affect the common Paracherodon axelrodii.

The current work presented tries to quantitatively measure the diversity of parasitic fauna found on the Paracherodon axelrodii that arrives weekly from Barcelos to the farm of a tropical fish exporters in the city of Manaus. Further, this study followed the prophylactics treatment techniques for ectoparasites to measure its efficiency one month after reception of these fish during this quarantine period.

The Paracherodon axelrodii is the main species exported as tropical fish from the Amazon region, it corresponds to 70% of fish exported from the Amazon state [7]. This species is endemic to the Negro river, found in flooded forests (igapos) and small creeks (Iguarapes) [8] and is sensitive to ecosystem change and parasitic infection. According to Marshall et al. [8] the Paracherodon axelrodii is omnivorous and not selective in its diet. The fisheries of tropical fish represented 60% of the income of municipalities like Barcelos and Santa Isabel [9].

The objective of this study is to prove that the prophylactic treatment used at the local tropical fish export company, developed in partnership with local research institutions, improves the quality of the fish exported from the amazon region.

Methods

Two sets of fish of 1000 each were analysed from the secondary affluent of the Negro River, Daracoa, and located 60 Km upriver from the city of Barcelos. The company, Turkys Aquarium, received the fish on its farm during the months of February and March/2010. Upon arrival, 100 fish were removed from the incoming batch and euthanized using the French method (decapitation of the head) for parasitological analysis. The remaining 900 fish were placed on tanks for a month in order to receive the appropriate prophylactic treatment.

The treatment consisted of 20 ml malaquite green and acryflavin, 30 ml formalin, 350 ml oxitetraciclin, NaCl 20 ppm, and 2 ml/600 l of Ivomek (IVERMECTINA). The medication quantities were indicated on Stoskopf [3]. In order to proceed with treatment, all excrements were removed from the bottom of the tank and the water flow was stopped for 8 hours during the treatment, according to Stoskopf [3] recommended discontinuation of water flow during treatment administration After this period, the water was reopened to allow for a change of water and removal of the medicines. Water parameters remained constant during the whole process of the treatment (pH 4, 5; conductivity 10 microns, and temperature 27[degrees]C). After month of the treatment, a batch of 100 fish was removed from the 900 fish that received the treatments in order to test for parasites occurrence. After looking at the total amount for each parasitic species type, the average occurrence was calculated per parasitic species per batch [10]. The parasitic identification was based on the keys from Stoskopf [3].

Results

A total of 400 fish (200 after arrival and 200 one month in quarantine and after chemical treatment) were analysed. The average abundance of parasites on the total batch was calculated by dividing the total number of parasites of a single species found on the total batch by the total number of possible hosts (numbers of individuals checked) [10]. The parasitic load was significantly lower after treatment and in most cases the incidence was reduced to zero. Five species of parasites were found on the batches. These were common species of ectoparasites, typical in Amazonian fish (Table 1). It is possible that if techniques of eletromacroscopy were used more species would have been found [11]. Apart from the camalanus, the research found a substantial decrease on the amount of parasites on the verified batches received [12].

Discussion

We found a positive correlation between the treatment and reduction in parasite abundance. The proportion of medication used was 10-20 % lower than that proposed by Stoskopf [3] because of different water characteristics (chemical and physical properties) from those tested in the book, since it was written for temperate regions. In addition, the high effectiveness of the treatment can be attributed to the fact that the fish being tested were from natural bodies of water. This meant they were naive for any type of chemical treatments and the probability of the parasites being resistant to treatment was null [1].

The only parasite for which the treatment did not appear to work was Camalanus. The quantity of parasites was not reduced in any of the trials, possibly because low dosage. Ivomek was placed in the water together with the other medications, however, the proper way to use this medication is via oral ingestion where the medications has to be mixed with the food given to the infected fish [3]. The same author mentions that it is possible to treat camalanus with water bath, but the best treatment is via oral ingestion. Further work has to be done in order to find the best application method to reduce abundance of camalanus [13-18].

Discovering new treatment methods helps improve the health and quality of the fish being exported from the Amazon region, reducing losses on all stages of this production chain and making the fish better accepted on both national and international markets due to its good quality and resistance. This can be true for tropical and edible fish markets, both a significant source of income for local companies, government and local riverine people. When done properly, this trade can consist of a sustainable usage of the forest [18,19].

http://dx.doi.org/10.4172/21503508.1000117

Acknowledgment

We would like to thank the people of Barcelos, the Turkys Aquarium staff, LEEM/INPA, UEA, FAPEAM and the Muraki foundation for financial support. We would like to dedicate this work to the "piabeiros" that for many years have spread the color of the forest throughout the world, preserving it at the same time.

References

[1.] Bassleer G (2005) Fish medicine: from fisherman/breeder to hobbyist. Ofi journal, Jubilee: 52-54

[2.] Hoare DJ (2000) The social organization of free-raging fish shoals." Oikos 89: 546-554

[3.] Stoskopf M (1993) Fish medicine. New York, WB Saunders company.

[4.] Fischer CJ (2004) Os parasitas do tambaqui, colossoma macropomum (cuvier, 1818) (characiformes: characidae) do medio rio solimoes (am) e do baixo rio amazonas (pa) e seu potencial como indicadores biologicos." Acta Amazonica 33: 651-662.

[5.] Malta JCO (2001) Infestacoes macicas por acantocefalos, neoechinorhynchus buttnerae golvan, 1956, (eoacanthocephala: neoechinorhynchidae) em tambaquis jovens, colossoma macropomum (cuvier, 1818) cultivados na amazonia central. Acta Amazonica 31: 133-143.

[6.] Tavares-dias M (2009) Protozoarios e metazoarios parasitos do cardinal paracherodon axalrodii schultz, 1956 (characidae), peixe ornamental proviniente de exportador de manaus, estado do amazonas, brasil. Acta Scientiarium, 31: 23-28.

[7.] Anjos (2009) Exportacao de peixes ornamentais do estado do amazonas, bacia amazonica, Brasil. b. Inst. Pesca 35: 259-274.

[8.] Prang G (2001) Aviamento and the ornamental fishery of the rio negro. Brazil: implications for susteinable use. Conservation and management of ornamental fish resources of the rio negro basin--project piaba. Amazonia, brasil university of amazonas press.

[9.] Chao NL, Petry P, Prang G, Sonneschien L, Tlusty M (2001) Ecolabeling and Green Certification for Effective Fisheries Management--An Analysis.

[10.] Bush AO (1997) Parasitology meets ecology on its own terms. Journal of Parasitology, 83: 575-583.

[11.] Alves DR (2000) Ocorrencia de camallanus cotti (nematoda: camallanidae) parasitando o guppy, poecilia reticulata (osteichthyes: poecilidadae). Revista Universidade Rural 22: 77-79.

[12.] Alves DR (2001) Metacecarias de clinostomum marginatum (digenea: clinostomidae) em acara-bandeira, pterophyllumscalare (osteichthyes:cichlidae) no estado do rio de janeiro, Brasil. Parasitologia al dia 25: 70-72.

[13.] Conservation and management of ornamental fish resources of the Rio Negro basin, Amazonia, Brazil--Project Piaba. Manaus: EDUA: 301

[14.] Cho SH, Kim CI (2009) Effect of dietary inclusion of various sources of additives on growth and body composition of juvenile olive flounder paralichthys olivaceus. Aquaculture Research 40: 625-629.

[15.] Ferraz E (1999) Management and disease of the ornamental fish exported from the rio negro basin. Biology of tropical fish. A. L. Val and v. M. Almeida-val. Manaus, inpa: 350

[16.] Ferraz E, Sommerville (1998) Pathology of piscinoodinum sp., parasites of the ornamental freshwater catfishes corydora spp.and brochis splends." diseases of aquatic organisms 33: 43-49.

[17.] Fujimoto R (2006) Avaliacao de tres diferentes metodos para controle de monogeneticos e capillaria sp. Parasitos de acara-bandeir, pterophyllum scalare. Boletim do Instituto 32: 183-190.

[18.] Magurran AE, Queiroz HL (2003) Partner choice in piranha shoals." Behaviour 19. Walker I (2004) The food spectrum of the cardinal--tetra (paracheirodon 140: 289-299. axelrodi, characidae) in its natural habitat. Acta Amazonica 34: 69-73.

Zehev Schwartz Benzaken (1) *, Clycia Pereira de Araujo Lima (1) and Vera Maria Fonseca de Almeida Val (2)

(1) University of the State of Amazonas, Brazil

(2) National Institute of Amazonian Research, Brazil

* Corresponding author: Zehev Schwartz Benzaken, University of the State of Amazonas, Brazil, Tel: +972549535450; E-mail: zehevbenzaken@gmail.com

Received January 06, 2015; Accepted February 24, 2015; Published February 28, 2015
Table 1: Average abundance of the occurrence of parasites
on two different batches processed at different times.

                      Abundance          Abundance
                    pre-treatment      post-treatment
                     infections          infections

                 1 Batch   2 Batch   1 Batch   2 Batch

Dactilogyrus      0,01        0         0         0
Camalanus         0,05      0,01      0,04      0,01
Capillaria        0,12        0         0         0
Piscinoodinium     2,5      0,25        0       0,04
Gyrodactylus      0,04        0         0         0
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Article Details
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Title Annotation:Research Article
Author:Benzaken, Zehev Schwartz; de Araujo Lima, Clycia Pereira; de Almeida Val, Vera Maria Fonseca
Publication:Fisheries and Aquaculture Journal
Article Type:Report
Date:Jan 1, 2015
Words:1910
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