Pumps, Channels, and Transporters: Methods of Functional Analysis.
Pumps, Channels, and Transporters: Methods of Functional Analysis
Edited by Ronald J. Clarke and Mohammed A. A. Khalid
Chemical Analysis; Volume 183
Chemists describe experimental methods for resolving the kinetics and dynamics of ion pumps, channels, and transporters in proteins. They specifically exclude structural methods such as X-ray crystallography and electron-microscopy, in order to focus on electrical methods to detect the currents and membrane voltage changes that the proteins produce. They also consider complementary techniques based on spectroscopy and radioactivity. Among the topics are analyzing ion permeation in channels and pumps using patch-clamp recording, recording pump and transport activity using electrophysiology based on solid-supported membranes, nuclear magnetic resonance spectroscopy, time-resolved and surface-enhanced infrared spectroscopy, and long timescale molecular simulations for understanding ion channel function. ([umlaut] Ringgold, Inc., Portland, OR)