Proteases from entamoeba spp. and pathogenic free-living amoebae as virulence factors.
Amoeba is a general name that is used for protists that form a large and diverse assemblage of eukaryotes that are characterized by various types of pseudopodia [1, 2]. Some amoebae are pathogenic and even parasitic to human and other vertebrate hosts. The four amoebae that are dealt with in this paper have been classified under two Super Groups, Amoebozoa and Excavata, as follows: (a) Entamoeba, Acanthamoeba, and Balamuthia are classified under the Super Group Amoebozoa; (b) Naegleria fowleri is classified under Super Group Excavata [1, 2]. The genus Entamoeba includes several species, such as E. histolytica, which causes amoebiasis, an infection in the gut characterized by invasion of the intestinal mucosa that occasionally spreads to other organs, mainly the liver, and E. dispar and E. moshkovskii, which are morphologically similar to E. histolytica and have been recently recognized as separate species. Although E. dispar and E.moshkovskii have no apparent invasive potential, they exhibit some pathogenicity [3, 4]. Molecular phylogeny analysis places the genus Entamoeba on one of the lowermost branches of the eukaryotic tree, closest to Dictyostelium . Although Entamoeba, originally thought to lack mitochondria, nuclear-encoded mitochondrial genes and a remnant organelle have now been identified. The establishment of E. dispar and E. moshkovskii as distinct, but closely related, species has profound implications for the epidemiology of amoebiasis because most asymptomatic infections are now attributed to these noninvasive amoebae .
Free-living amoebae belonging to the genera Acanthamoeba, Balamuthia, and Naegleria are responsible for opportunistic and nonopportunistic infections in humans and other animals. The distinction between parasitic and free-living protozoa is generally sharp, with organisms falling readily into one or the other category. Some of the free-living amoebae are unusual in that they straddle the line separating the two groups of organisms and yet are as destructive as any of the classic parasitic protozoa . Unlike their parasitic counterparts, these free-living amoebae are not well adapted for parasitism. Furthermore, as free-living forms with a broad distribution, they are not dependent upon a host for transmission and spread. Unlike the parasite E. histolytica, free-living amoebae are mitochondrion-bearing aerobic organisms that can complete their respective life cycles in the environment without a host; thus, these organisms have been called amphizoic amoebae in recognition of their ability to live endozoically, although they are capable of free-living existence .
All pathogenic amoeba species have in common the capability to phagocytose bacteria, erythrocytes, and cell detritus. The major virulence factors are adhesins, toxins, amoebapores, and proteases, which lead to the lysis, death, and destruction of a variety of cells and tissues in the host. Because of their relevance to amoebic pathogenesis, proteolytic enzymes are of particular interest. Proteases are important in tissue invasion, migration, and host pathology. The main goal of this paper is to review the proteases of the Entamoeba genus and of free-living amoebae.
2. Entamoeba histolytica
Amoebiasis is a human parasitic infection caused by Entamoeba histolytica, an extracellular protozoan. Cysts are transmitted through the fecal-oral route by contaminated water or food. Parasite destruction of host tissues appears to be the basis of amoebiasis, which leads to invasive disease pathologies such as intestinal amoebiasis, which is mainly characterized by lesions in the colon that produce fever, abdominal pain, dysentery, and ulcerative colitis with mucous and blood. Amoebae can spread through the portal vein to other organs such as the liver, lungs, kidneys, and brain. Hepatic amoebiasis is characterized by liver abscesses that can be fatal [53-57].
Amoebiasis is the third leading cause of death due to parasites, after malaria and schistosomiasis. Amoebiasis presents a high index of morbidity and mortality, mainly in developing countries. According to the World Health Organization (WHO), 500 million people are infected with amoebae; 10% of infected individuals have virulent E. histolytica, resulting in 40,000-100,000 deaths annually . E. histolytica infects only humans. The life cycle of E. histolytica consists of two main stages: the trophozoite (also called amoeba) or invasive form, and the cyst or infective form. Cysts can tolerate the stomach acidic pH and excyst in the terminal ileum. Trophozoites colonize the large intestine, and in later phases of amoebiasis, they invade the epithelium and mucosa. In response to unknown stimuli, amoebae undergo morphological and biochemical changes that lead to the formation of new cysts, which are eliminated in the feces, completing the cycle [53, 55, 56].
The cytopathogenic effect caused by E. histolytica trophozoites is multifactorial. Intestinal flask-shaped ulcers, a hallmark of amoebic colitis, are characterized by severe damage to enteric cells as well as migration to the lamina propria and blood vessels [57, 58]. The contact between trophozoites and target cells appears to be the first step for cell lysis and phagocytosis. Several molecules are involved in this interaction, including the 260 and 220 kDa lectins and 112 kDa adhesin,which participates in the adherence to epithelial cells and erythrocytes [8, 59-63]. It has been proposed that for the initial amoeba contact or adhesion, surface carbohydrates on the target cell are recognized by specific molecules from the parasite. One of the better studied amoebic molecules is the Gal/GalNAc lectin, which mediates binding to host carbohydrate determinants that contain galactose and/or N-acetyl-D-galactosamine (GalNAc) [64, 65]. Adherence to colonic mucosa is conducive to the continued reproduction of parasites and tissue damage by the products secreted by amoebae, such as the pore-forming peptide amoebapore , which permits a massive influx of extracellular Ca+2 that is combined with the release of amoebic proteases at the site of contact, with the subsequent degradation of substrates. Once the targets are partially digested, the amoeba internalizes the cell debris and substrate fragments by phagocytosis . Other proteins also contribute to host cell binding on target cells and destruction, such as phospholipases [68, 69].
2.1. Proteases of E. histolytica and Their Role in Virulence. Studies of E. histolytica proteinases (proteases) have mainly been performed in the strain HM-1:IMSS from axenically grown trophozoites. De la Torre et al.  isolated this strain from cysts of a Mexican patient suffering from intestinal amoebiasis. Most of the cellular and molecular studies of E. histolytica throughout the world, including the genomic sequence, have been performed with this strain. It has been cultured for years and passed through the liver of Syrian golden hamsters, an experimental model in which hepatic abscesses are reproduced to maintain and increase the virulence of E. histolytica.
Cysteine proteases (CPs) are the predominant proteolytic activity associated with pathogenicity in E. histolytica, based on many studies in which the degradation of different substrates has been investigated, including purified proteins of the extracellular matrix (ECM), immunoglobulins, complement, and mucin. Figures 1 and 2 show the role of these proteases during intestinal amoebiasis and blood vessel transit of trophozoites, respectively. Animal model studies have confirmed that CPs have an important role in virulence; E. histolytica mutants impaired in genes encoding CPs have a diminished ability to produce hepatic abscesses [71, 72]. Figure 3 shows the role of proteases during amoebic liver abscess. EhCPs are expressed both intracellularly and extracellularly and are referred to as cathepsin-like enzymes because their structure is similar to that of cathepsin L; however, their substrate specificity resembles that of cathepsin B [34, 73-75]. Some proteases have been characterized as surface localized; hence, they have the potential to contribute to host tissue breakdown in vivo  (Figure 4). E. histolytica most studied proteases are summarized in Table 1.
2.1.1. Purification and Cloning of the Main Cysteine Proteases from E. histolytica. Several genes encoding E. histolytica CPs have been cloned. In 1990, Eakin et al.  amplified CP gene fragments from trophozoites by PCR from genomic DNA using degenerate oligonucleotide primers designed based upon amino acid sequences flanking the active site of CPs conserved in eukaryotic cells. The amplified DNA fragments were subcloned, and sequence analysis and alignment revealed significant sequence similarity to other members of the eukaryotic CP family (45% identical to chicken cathepsin L). The cysteine, histidine, and asparagine residues that form the catalytic triad are also conserved. Subsequently,  isolated three genes from the strain HM-1:IMSS, designated as acp1, acp2, and acp3. These genes have since been renamed, and the prefix "eh" has been added, so that these genes are now denoted ehcp1 (acp3), ehcp2 (acp2), and ehcp3 (acp1). Next, we will review studies of the purification and cloning of E. histolytica CPs. Most of these studies were published before the establishment of an amoebic protease nomenclature or include experiments that only demonstrate degradation of the substrate; for these studies, we will focus on proteolytic activity and Mr.
By screening a genomic library from E. histolytica,  identified six genes encoding prepro-forms of CPs (ehcp1-ehcp6). The nucleotide sequences of these genes differed by 40%-85%, and three of the genes, ehcp1, ehcp2, and ehcp5, exhibited the highest levels of expression. Amoeba lysates were analyzed for CP activity against a synthetic substrate. Enzyme purification revealed that EhCP1, EhCP2, and EhCP5 are the main proteases, representing approximately 90% of CP expression. Tillack et al.  analyzed 79 of 86 genes encoding putative proteases by microarray hybridization. Of these, 50 encode CPs of various families, all of which belong to the clan CA. Interestingly, under standard culture conditions, the authors consistently observed the expression of only 20 genes. Very few differences were apparent among the isolates from asymptomatic and amoebic disease-affected individuals, even though the isolates had different geographic origins. Again, only three peptidase genes were expressed at high levels: ehcp1, ehcp2, and ehcp5. Accordingly, in the less pathogenic strain HK-9, the expression of EhCP5 was decreased by 2.3-fold. However, these E. histolytica isolates were grown under axenic conditions, and the expression profiles of CPs have been shown to adapt to different stimuli. This adaptation was confirmed in a transcriptional analysis of trophozoites isolated from the colons of infected mice versus trophozoites cultured in vitro. In contrast to what is observed in axenic cultured trophozoites, EhCP4, EhCP6, and EhCP1 were the most upregulated CPs during invasion and colonization of mice .
EhCP1 (Amoebapain). A thiol-dependent protease was identified and partially purified by means of ammonium sulfate fractionation, gel filtration, and isoelectric focusing. The CP had Mr of 21 [+ or -] 2 kDa by gel chromatography. The maximal activity occurred at pH 4.4, but the CP was active at pHs of 3.4 and 8.5 . Its proteolytic potential toward native substrates and bovine insulin B-chain was examined [81, 82]. The CP was purified by two gel chromatography steps, ion exchange chromatography on DEAE-cellulose, and affinity chromatography on organomercurial-Sepharose. The purified protease was monomeric with a Mr of 27 [+ or -] 2 kDa ; its activity required an arginine at the P2-position of the substrate. The protease was able to digest native collagen type-I, with an initial attack at the alpha 2-chain . EhCP1 is located on the cell surface .
By using an antiserum against the 27 kDa protease, a cDNA clone was obtained. Northern blot analyses suggested that the CP is more expressed in pathogenic isolates than in nonpathogenic isolates . Accordingly, using a purification method similar to that described earlier, cathepsin B activity was obtained from virulent strains (HM-1:IMSS and Rahman), which yielded significantly more activity per milligram protein than less virulent strains (HK-9, Laredo, and Huff). These results suggest a correlation between amoebic cathepsin B and the pathogenesis of amoebiasis .
EhCP2 (Histolysain). A one-step method for the purification to homogeneity of the histolysain of a soluble parasite extract by affinity chromatography to a synthetic substrate has been reported. The enzyme showed an apparent Mr of 26kDa by SDS-PAGE and 29 kDa by gel chromatography. Its optimum pH varied widely with synthetic substrates, from 5.5 to 9.5. EhCP2 did not degrade type-I collagen or elastin but was active against cartilage proteoglycan and kidney glomerular basement-membrane collagen. EhCP2 also detaches cells from their substrates in vitro and could play a role in tissue invasion. This EhCP is located on the cell surface as well as in internal membranes .
EhCP3 (ACP1). EhCP3 (ACP1) was obtained by PCR amplification of E. histolytica genes using primers based on conserved structural motifs of eukaryotic CPs. It was initially reported to be present only in E. histolytica , but a related homologue gene (edcp3) with 95% identity has been detected in E. dispar [35, 84]. EhCP3 (308 amino acids) is synthesized as a preproenzyme, in which 13 amino acids form a signal peptide and 79 form a profragment (http://www.uniprot.org/uniprot/P36184). As a (His) 6-tagged protein, themature enzymemigrates with an apparent Mr of 31 kDa . The enzyme was cloned and expressed in baculovirus, and purified rEhCP3 was active in a broad pH range of 4.5-8.0 and had a neutral optimal pH. EhCP3 was sensitive to E-64, a specific CP inhibitor. The sequence of EhCP3 is most similar to that of cathepsin L, while its substrate preference for positively charged amino acids in the P2 position is consistent with cathepsin B . Although EhCP3 is a relatively low-level transcript in HM-1:IMSS, after EhCP2, EhCP5, and EhCP1 , its expression is 100-fold higher in the Rahman strain .
Using confocal microscopy with monoclonal antibodies (mAbs) to EhCP3, EhCP3 was localized primarily in the cytoplasm. Following erythrophagocytosis, EhCP3 colocalizes with phagocytic vesicles . The presence of a homologous gene in E. dispar, in addition to its localization in E. histolytica phagosomes, has led to the hypothesis that the primary function of EhCP3 may be associated with the digestion of nutrients rather than virulence; however, further studies (i.e., knockdown experiments) are needed to clarify its function.
EhCP4. EhCP4 was described as a positive clone in a genomic library screened with an oligonucleotide probe derived from a sequence conserved between ehcp1-3 and ehcp5. However, by Northern blot, the expression of ehcp4 and ehcp6 was barely detected in amoebae grown in vitro. Analogous genes are present in E. dispar, but just as what occurs with E. histolytica, their expression is almost undetectable in vitro . In a recent transcriptional analysis comparing E. histolytica HM-1:IMSS isolated from mouse intestine with cultured amoebae from the same strain, both EhCP4 and EhCP6 were upregulated in trophozoites in the mouse gut (20-35 fold and 10-fold increased, resp.); in fact, ehcp4 (ehcp-a4) was the most upregulated CP gene during invasion and colonization in a mouse cecal model of amoebiasis . Upregulation of ehcp4 in vivo correlated with the finding that coculture of amoebae with mucinproducing T84 cells increased ehcp4 expression up to 6-fold. EhCP4 was cloned, including the prodomain and catalytic domain, for expression in bacteria. EhCP4 was purified and underwent autocatalytic activation at acidic pH but had greatest proteolytic activity at neutral pH. The calculated Mr of the mature enzyme was 23 kDa, but the apparent Mr was 26 kDa, as assessed by SDS-PAGE .
Sequence alignment and computer modeling confirmed that EhCP4 is a member of Clan CA, C1A subfamily with a cathepsin L-like structure [73, 75, 87], similar to previously characterized EhCPs. However, unlike previously characterized EhCPs, which have a preference for cathepsin B or L substrates with arginine in the P2 position, EhCP4 displays a unique preference for valine and isoleucine at P2 . EhCP4 is localized in the peri- and intranuclear regions as well as in variable-sized vesicles in the cytoplasm. In axenic amoebae, EhCP4 is primarily cytoplasmic, but in those isolated from infected mouse ceca, EhCP4 is found in both the nuclear and cytoplasmic extracts, suggesting a role for EhCP4 in the cell cycle or differentiation, as is the case for higher eukaryotic CPs with a nuclear localization [88-90]. Following coculture with colonic cells, EhCP4 appeared in acidic vesicles and was released extracellularly. Indeed, in the infected murine cecum, immunofluorescence staining of EhCP4 yielded strong signals in vesicle-like structures located in discrete surface areas. Thus, EhCP4 is apparently secreted in vivo .
EhCP4 cleaves several host proteins in vitro, although in agreement with the different substrate specificity, the predicted digestion patterns differ from those of EhCP1. EhCP4 degrades C3, unlike EhCP1-mediated C3 processing, which produces an active C3b molecule [17, 18]. EhCP4 also degrades IgA, IgG, pro-IL-18, villin-1, and laminin1 . Thus, like EhCP1, EhCP4 could play an important role in destroying the integrity of tissues and evading the immune system and may contribute to the inflammatory response in amoebic lesions . Based on the substrate preference and homology modeling, a specific vinyl sulfone inhibitor WRR605 was synthesized, which selectively inhibited rEhCP4. Treatment with WRR605 notably decreased the amoeba burden and intensity of cecal inflammation in a mouse cecal model of amoebiasis . Together, these results suggest that EhCP4 is an important virulent factor in E. histolytica and a promising therapy target.
EhCP5. A soluble protein of Mr 30 kDa with an affinity for membranes was purified from amoebic extracts. N-terminal sequencing and subsequent molecular cloning revealed that it was a member of the CP family of E. histolytica. The enzyme strongly associates with the membrane, retaining its proteolytic activity, and produces cytopathic effects on cultured monolayers. The 3D structure of EhCP5 revealed the presence of a hydrophobic patch that may account for membrane association potential of the protein. Immunocytochemical localization of the enzyme at the amoeba surface suggests a potential role in tissue destruction. The gene encoding EhCP5 is not expressed in the closely related but nonpathogenic species E. dispar [87, 92]. Trophozoites of the virulent strain transfected with an antisense gene encoding EhCP5 had only 10% activity but retained their cytopathic effect on mammalian cell monolayers and were incapable of inducing the formation of liver lesions in hamsters. However, this activity cannot be attributed only to EhCP5 because EhCP5 antisense mRNA also inhibited the expression of other CPs, which maybe due to a high degree of sequence homology and conservation of residues critical for protease function [71, 93].
EhCP5 has been confirmed to degrade a broad spectrum of biological and synthetic substrates such as mucin, fibrinogen, collagen, hemoglobin, bovine serum albumin, gelatin, IgG, Z-Arg-Arg-pNA, and Z-Ala-Arg-Arg-pNA but not Z-Phe-Arg-pNA. The identification of rEhCP5 as a CP was determined by using specific inhibitors . EhCP5 activity and mRNA levels were analyzed in E. histolytica samples isolated from patients presenting different clinical profiles. The degree of virulence of the isolates, determined in hamster livers, correlated well with the clinical form of the patients and with the culture conditions. EhCP5 mRNA levels were also determined in fresh samples of amoebic liver abscesses. Differences were not observed in the levels of EhCP5 mRNA and CP specific activity among the cultured samples. However, different levels of EhCP5 mRNA were observed in amoebae freshly isolated from hepatic lesions. These results emphasize the importance of EhCP5 for amoeba virulence and the need for additional studies to validate the mechanisms involved in the pathogenesis of amoebiasis .
EhCP5 was expressed in the bacterium Escherichia coli as a proenzyme and purified to homogeneity under denaturing conditions in the presence of guanidine-HCl. EhCP5 was renatured in buffer containing reduced and oxidized thiols, which led to a soluble but enzymatically inactive proenzyme. Further processing and activation was achieved in the presence of DTT and SDS. The recombinant enzyme (rEhCP5) was indistinguishable from native EhCP5 purified fromamoebic lysates. Under reducing and nonreducing conditions, rEhCP5 exhibited Mr of 27 and 29 kDa, respectively, with the same optimal pH and similar specific activity against azocasein .
EhCP112. EhCP112 is a papain-like proteinase . It was first identified by using a mAb recognizing a protein present in wild-type trophozoites but missing in an adherence-deficient mutant. The mAb inhibited adhesion of amoebae to target cells, and a 112 kDa protein was identified by Western blot . The DNA encoding this molecule was isolated from a genomic library, revealing that the 112 kDa adhesin is formed by two polypeptides (49 kDa and 75 kDa) encoded by two different genes separated by 188 bp. The 49 kDa polypeptide is a CP (EhCP112), whereas the 75 kDa protein has a domain involved in the adherence of amoeba to target cells (EhADH112); both proteins form a complex called EhCPADH. Within this complex, the peptides could be joined by covalent or strong electrostatic forces, and their proximity in the genome suggests a coregulated expression .
EhCP112 (446 amino acids) is synthesized as a preproenzyme in which 19 amino acids form the signal peptide and 112 amino acids form the profragment. The mature protease has a putative Mr of approximately 34 kDa. EhCP112 contains a transmembrane segment domain characterized by the catalytic triad (C, H, and N), in addition to an RGD motif, which is an integrin attachment domain . A previous report suggests that the majority of E. histolytica CP genes, including the ehcp112 gene, are not expressed during in vitro culture . However, Northern blot and RT-PCR assays indicate that the ehcp112 gene is expressed in trophozoites [60, 96]. Different culture conditions or different pathogenicities of trophozoites could be responsible for the distinct results.
EhCPADH is located in cytoplasmic vesicles and the plasma membrane and is also secreted. EhCPADH is translocated from the plasma membrane to phagocytic vacuoles during phagocytosis . This implies a function in nutrient acquisition. However, EhCPADH has been associated with amoebae virulence. Antibodies against this complex inhibit adherence, phagocytosis, and destruction of MDCK monolayers by live trophozoites and extracts. Antibodies also greatly reduce the ability of amoebae to produce liver abscesses in hamsters [59, 60, 97-99].
To elucidate the role of EhCP112 in virulence, it was cloned, expressed in bacteria and purified. rEhCP112 degrades collagen type I and fibronectin and destroys cell monolayers (MDCK). rEhCP112 also binds to erythrocytes and digests human hemoglobin . rEhCP112 is active in a broad pH range (3-10), with the highest activity at pH 7.0 for azocasein and pH 6.0 for hemoglobin. EhCP112 was enzymatically active from5 to 60[degrees]C with amaximumactivity for both substrates at 37[degrees]C . Interestingly, the EhCP112 enzyme is immunogenic in patients with amoebiasis .
2.1.2. Proteases Involved in Trophozoite Adhesion. Leishmanolysin (Gp63) is a metalloendopeptidase that is essential for the virulence of Leishmania major. Leishmanolysin degrades ECM proteins during tissue invasion and prevents complement-mediated lysis of promastigotes [100, 101]. Two leishmanolysin homologues are encoded in the E. histolytica genome, but only one copy of the gene is present in the closely related E. dispar. The E. histolytica specific family member EhMSP-1 is a functional metalloprotease (MP) that is localized to the cell surface. Silencing EhMSP-1 expression dramatically increases amoebae adherence to live and dead cells, reducing mobility in cell monolayers and increasing phagocytosis. The knockdown of EhMSP-1 also reduces cell monolayer destruction but has no effect on the lysis of Jurkat lymphocytes. Thus, one possibility is that this phenotype could be the consequence of defective adherence because increased adherence may reduce the ability to move, which, in turn, causes the amoebae to be less able to destroy cell monolayers. The ultimate mechanism by which EhMSP-1 affects adherence remains unknown. However, it is known that EhMSP-1 knockdown does not affect steady-state surface abundance of previously identified adhesins such as the GalNAc-specific lectin and the serine-rich E. histolytica protein (SREHP) [102-104]. The ability of the CP inhibitor E-64 to almost completely block cell monolayer destruction suggests that EhMSP-1 silencing affects monolayer destruction indirectly and that EhMSP-1 itself likely does not directly degrade cell monolayers [91, 102, 105]. Apparently, EhMSP-1 plays no role in in vitro resistance to complement, in contrast to leishmanolysin.
The rhomboid-like proteins are a large family of seven-pass transmembrane serine proteases (SPs) that were first identified in Drosophila melanogaster and whose active site lies within the lipid bilayer, allowing them to cleave transmembrane proteins [106, 107] and, hence, trigger signaling events . Substrates of rhomboid proteases are largely single-pass transmembrane proteins with transmembrane domain containing helix-breaking residues . The E. histolytica genome encodes four rhomboid-like genes, of which only a single gene, EhROM1, contains the necessary catalytic residues for proteolytic activity . In resting conditions, EhROM1 is localized at the trophozoite surface and in internal punctuate structures; upon erythrophagocytosis, it relocalizes in internal vesicles and, during surface receptor capping, in the base of the cap. The heavy subunit of the Gal/GalNAc lectin (Hgl) is a substrate of EhROM1 in vitro . EhROM1 knockdown leads to defects in both adhesion and phagocytosis but not cap formation or complement resistance.
Importantly, the reduced phagocytosis and adhesion phenotypes appear to be independent, implying that EhROM1 has distinct roles in both pathways. Interestingly, there were no significant changes in the expression or localization of the only known substrate, the heavy subunit of the Gal/GalNAc lectin, in EhROM1 knockdown parasites. Several scenarios have been proposed for how EhROM1 regulates parasite adhesion without affecting the expression or localization of the heavy subunit of the Gal/GalNAc lectin. EhROM1 may process a different adhesin or a different substrate that masks the Gal/GalNAc lectin adhesion, or it may play a role in signaling during the adhesion process by detaching the signaling integrin-like motif present in the cytoplasmic domain from the rest of the Gal/GalNAc lectin (Figure 5). Alternatively, EhROM1 may have a noncatalytic role in the adhesion process, as has been described for other rhomboid proteases [111, 112].
Cell-adhesion processes and proteolytic mechanisms function in a coordinated manner to provide directed cell migration and are critical at the molecular level; adhesion receptors (integrins) are displayed on the cell surface and bind to ECMproteins, whereas proteases (e.g., MPs and CPs) modify or degrade ECM components. However, some proteases interfere with cell adhesion and/ormigration in a nonproteolytic fashion [113-116]. In cell-adhesion proteins such as fibronectin, RGD motifs serve as ligand recognition sites for cell-surface receptors such as integrins. An RGD motif has been found in the pro-region of the lysosomal cathepsin X from higher eukaryotes, and apparently, this motif can mediate adhesion and migration processes via binding to integrins . Only EhCP5, EhCP18, and EhCP112 present this motif . The RGD motif is present in the pro-form of EhCP5 (EhPCP5); the RGD motif binds to [alpha](V)[beta](3) integrin on Caco-2 colonic cells and stimulates NF[keppa]B-mediated proinflammatory responses. Binding to this integrin triggers integrin-linked kinase- (ILK-) mediated phosphorylation of Akt-473, which binds and induces the ubiquitination of the NF-[keppa]B essential modulator (NEMO) (Figure 6). As NEMO is required for the activation of the IKK[alpha]-IKK[beta] complex and NF[keppa]B signaling, these events markedly up-regulate proinflammatory mediator expression in vitro in Caco-2 cells and in vivo in colonic loop studies in wild-type and Muc2(-/-) mice lacking an intact protectivemucus barrier. These results have revealed that the EhPCP5 RGD motif represents a novel mechanism by which trophozoites trigger an inflammatory response in the pathogenesis of intestinal amoebiasis .
As mentioned earlier, EhCPADH is a surface complex that binds to target cells [59, 60, 99]. The role of EhCP112 in adhesion could be limited to its association with the adhesin EhADH112, although EhCP112 contains the RGD domain, suggesting that it binds to cellular integrins [8, 118]. The parasite may utilize surface-bound proteases to interact with integrins via the RGD motif to tether and propel along the endothelium, much like immune cells crawling along the vasculature to survey blood contents for pathogens .
2.1.3. Proteases That Degrade Mucin. Mucous is the first layer that protects the colonic epithelium from potentially pathogenic viruses, bacteria, and parasites . Theability of amoebae to invade may be inhibited by the binding of mucin to lectin, which inhibits lectin activity, and by the physical barrier between the parasite and the intestinal epithelium created bymucin .MUC2is themajor gel-formingmucin secreted by goblet cells of the small and large intestines and is themain structural component of themucus gel [121, 122]. To gain access to the underlying epithelial cells, amoebae must first breach the protective mucous layer. This phenomenon has been observed in biopsies from patients with acute amoebic colitis  and has been confirmed in an ex vivo human colonic model of amoebiasis; after just two hours of incubation, the mucus layer was no longer observable, suggesting that it had been removed by amoebae . E. histolytica secreted CPs are responsible for degrading MUC2 ; cleavage at the major cleavage site is predicted to depolymerize the MUC2 polymers, thereby disrupting the protective mucus gel . In addition, the parasite encodes glycosidases that degrade mucin oligosaccharides . E. histolytica may use the concerted actions of glycosidases and proteases to disassemble the mucin polymeric network.
Trophozoites with reduced CP activity are ineffective in degrading and crossing the protective mucus layer produced by cell lines in culture [31, 93]. However, controversy remains about the effector protease(s) responsible for mucin degradation. Bioactive recombinant EhCP5 is capable of degrading purified native mucin [9, 31]. However, an EhCP5/amoebapore-silenced strain was able to cross the mucus barrier in an ex vivo colonic model but was unable to migrate within the mucosa. This behavior suggests that other proteases produced by the EhCP5/amoebaporesilenced strain appear to be involved in the removal of the colon mucin gel . Alternative effector proteases include EhCP1, EhCP2, and EhCP4 because their expression is increased after trophozoites are exposed tomucin [14, 127]. This suggests that the specific composition of mucin may affect the ability of E. histolytica CPs to degrade the mucous layer.
2.1.4. Alterations in Microvilli and Tight Junctions. After the mucus, the tight junction (TJ) complex constitutes the first barrier against the paracellular penetration of intestinal microorganisms. This barrier is formed by the plasma membrane-spanning proteins claudin and occludin, which are associated with the cytoplasmic proteins ZO-1, ZO-2, and ZO-3. E. histolytica is able to disturb the TJ complex from human colonic adenocarcinoma T84 and MDCK T23 cells. Trophozoites decrease the transepithelial electrical resistance in T84 cells and, to a lesser extent, in MDCK cells . The same group of researchers subsequently observed the dephosphorylation of ZO-2, loss of ZO-1 from ZO-2, and degradation of ZO-1 when amoebae were cocultured with T84 cells . By contrast,  analyzed the interaction between trophozoites and human Caco-2 cell layers and identified a crucial role for amoebic CPs in the prevention of villin proteolysis and associated microvillar alterations through the treatment of Caco-2 cell layers with inhibitors that completely block CP activity. Moreover, trophozoites of strains pSA8 and SAW760, which have strongly reduced CP activity, exhibited reduced proteolysis of villin in coculture with enteric cells, indicating that villin proteolysis is an early event in the molecular cross-talk between enterocytes and amoebae that causes the disturbance of microvilli. Both EhCP1 and EhCP4 are capable of degrading human villin in vitro, and thus they could play an important role in destroying the integrity of microvilli in vivo (Che He 2010). In addition,  reported that TPCK and TLCK, two SP and CP inhibitors, can affect E. histolytica proteases and prevent proteolysis of ZO-1, ZO-2, and villin in Caco-2 cells. Taken together, these results indicate that amoebae use their proteinases to overcome microvilli and tight junction barriers during the invasion of enteric cell layers.
2.1.5. Proteases That Degrade the Extracellular Matrix. Cysteine proteases have been demonstrated to act on a variety of substrates in vitro. In the host, parasite proteases facilitate tissue penetration by dissolving the intestinal mucosa and extracellular matrix (ECM) and freeing epithelial cells [7, 11, 27, 125, 130, 132-134]. More than 80% of patients suffering from amoebiasis express antibodies to trophozoite CPs . Interestingly, purified enzymes that degrade one of the ECM proteins have been found to cleave other ECM proteins, generally fibronectin, collagen, and laminin. The interaction of amoebae with ECM components results in the proteolysis and destruction of the connective tissue.
For 30 years, proteases degrading native type-I and type-III collagen fibers have been studied in cultured E. histolytica, although CPs were purified more recently. Collagenase is more active against type-I collagen. Three major fragments of 75, 50, and 25 kDa were obtained from type-I collagen upon incubation with amoebae. After 3 h of incubation, smaller fragments of degraded collagen were observed, possibly due to the action of other proteolytic enzymes . In other work, FPLC-anion-exchange and chromatofocusing chromatography were used to purify the major neutral proteinase from secretions of the cultured E. histolytica HM-1:IMSS strain. This proteinase is a CP with a subunit Mr of approximately 56 kDa, a neutral optimum pH, and a pI of 6. The importance of this enzyme in extraintestinal amoebiasis is suggested by its ability to degrade a model of connective tissue ECM as well as purified fibronectin, laminin, and type-I collagen. The enzyme caused a loss of adhesion of mammalian cells in culture, most likely because of its ability to degrade anchoring proteins. Experiments with a peptide substrate and inhibitors indicated that the proteinase preferentially binds peptides with arginine at P-1. It is also a plasminogen activator and could thus potentiate host proteinase systems . A 56 kDa band was identified by polyacrylamide-gelatin gel electrophoresis in 10 of 10 isolates from patients with colitis or amoebic liver abscesses but in only 1 of 10 isolates from asymptomatic patients. Pathogenic isolates appear capable of releasing significantly larger quantities of this CP, which is released during the course of invasive disease, as demonstrated by the presence of circulating antibodies detectable by enzyme-linked immunosorbent assay (ELISA). These studies support the importance of the 56 kDa CP in the pathogenesis of invasive amoebiasis .
The collagenase activity was mainly observed in electrondense granules. These granules were induced and secreted in response to the incubation of amoebae with collagen type I in vitro. A specific collagenase activity with Mr of 72 kDa was identified in crude extracts. We observed this activity in electron-dense granules; this activity could be related to actin cytoskeleton function because the cytoskeleton-altered amoeba strain BG-3 derived from the HM-1:IMSS strain exhibits lower collagenase activity [12, 137]. Incubation of amoebae with type-I collagen not only promotes collagenase activity but also increases the secretion of other CPs , and together with Ca2+, type-I collagen can induce the activation of several amoebic genes related to virulence factors, such as amoebapore C and EhCP5, along with the stress-induced protein HSP70 and the ribosomal protein L27a . In addition, a correlation between collagenolytic activity and virulence was observed when the levels of activity of different strains (virulent HM-1:IMSS and the less virulent 200-NIH and HK-9) [139-141] or of other virulence factors  were compared, suggesting that collagenase may play a role in the pathogenesis of amoebiasis. In a recent study, Chavez-Munguia et al.  demonstrated that electron-dense granules contain multiple CP activities.
Recently, the behavior of wild-type and EhCP5-silenced E. histolytica was compared on a 3D-collagen matrix and within human colon fragments for fibrillar collagen cleavage and migration. Interstitial collagen fibers within the connective tissue of the human colon, visualized by multiphoton and second harmonic generation signal imaging, presented a dense scaffold at the subepithelial level and a loose meshwork within the chorion. To penetrate the tissue, wild-type E. histolytica migrated on the dense scaffold that remained intact, reached the crypt of Lieberkhun, migrated along, and then disorganized the loose scaffold to escape into the mucosa. However, in vitro, EhCP5 was not required for collagenase activity and migration through the matrix but was necessary within the tissue environment for collagen meshwork remodeling and subsequent invasion. The data indicate that subsequent steps of the invasion relay with ECM destruction require human components that are induced or activated in the presence of EhCP5 .
The major 27-29 kDaCP has been studied since 1989. This cystatin-inhibitable CP degrades the ECM proteins collagen types IV and V as well as laminin and fibronectin with different velocities and specificities under native conditions. Whereas the degradation of fibronectin and laminin proceeds rapidly, collagen breakdown occurs slowly and incompletely. This CP represents, by far, the highest portion of soluble proteolytic activity in E. histolytica and is sufficient to destroy the host ECM. Similar results have been reported for the 27 kDa CP with respect to the binding specificity for immobilized laminin over collagen and fibronectin. Inactivation of the CP with the active-site inhibitor E-64 abolishes laminin binding by the enzyme, and conversely, laminin inhibits the cleavage of a fluorogenic dipeptide substrate of the CP, suggesting that the substrate binding pocket of the enzyme binds to laminin. Furthermore, the addition of laminin but not fibronectin or collagen to amoebae significantly reduces amoebic liver abscess formation in severe combined immunodeficient (SCID) mice, further supporting the assumption that CPs play an important role in amoebic pathogenesis .
An in vitro model was developed to analyze the adhesion to and cleavage of human fibronectin-covered surfaces. This model revealed the specificity of the binding and occurrence of structural and biochemical events in amoebae that participate in and promote adhesion to the substrate and its degradation. Similar results were obtained with laminin and Matrigel [144, 145]. A putative amoebic fibronectin receptor with Mr of 37 kDa was identified. Adhesion to fibronectin triggers the release of proteases, which facilitates the local degradation of the substrate [144, 146]. Some of the proteases may generate fragments with chemotactic and chemokinetic properties that are able to promote the binding and locomotion of amoebae . In later studies, ECM proteins have been shown to be cleaved in vitro by identified CPs; collagen is degraded by EhCP1 , EhCP2 , and EhCP112 , fibronectin by EhCP112 , and laminin by EhCP4 .
2.1.6. Cytopathic Activity. Several studies have shown a direct correlation between E. histolytica CPs and tissue damage; as CP activity decreases, tissue damage decreases. This relationship has been tested using trophozoites with low levels of CPs in an amoebic liver abscess hamster model  or in human colonic xenografts . The inhibitor of CP activity E-64 reduces abscess formation in SCID mice , and specific inhibitors of EhCP4 reduce cecal inflammation , or specific inhibitors of EhCP1 in human colonic xenografts . In the opposite strategy, overexpression of ehcp5 but not ehcp1 or ehcp2 significantly increased abscess formation in gerbils .
The results of in vivo experiments reflect all of the pleiotropic effects of EhCPs in the pathogenic mechanism of amoebiasis. Some research has sought to probe the direct cytopathic effect of each EhCP, but the resulting data are controversial. Cytopathic activity, as measured by in vitro monolayer disruption, was increased in EhCP5- and EhCP2-overexpressing E. histolytica [149, 150]. However, silencing of the major ehcp genes (ehcp1, ehcp-2, ehcp-5, and ehcp7) or a reduction of expression to only 10% of the regular levels of CP activity did not influence trophozoite-induced cell monolayer disruption, suggesting that these peptidases are dispensable for cytopathic activity [93, 151]. All of these results were obtained using live intact trophozoites; however, lysates of virulent E. histolytica have also been shown to destroy monolayers . Lysates of amoeba with only 10% of the total EhCP activity exhibited very poor activity against monolayers. This result was confirmed using the general CP inhibitor E-64; while CPs of viable trophozoites are responsible for 50% of cytopathic activity, this inhibitor completely blocks the cytopathic activity of E. histolytica lysates . The effects of E-64 suggest that CPs are not the main virulence factor involved in the destruction of monolayers by intact trophozoites, but they must play some role, particularly whenever there is also some lysis of the trophozoites .
Nevertheless, these data should be interpreted cautiously becausemonolayer destruction was assayed; these assays cannot distinguish between monolayer releases reflecting ECM degradation from actual cell death . The investigation of the response of cultures of a human liver sinusoidal endothelial cells (LSECs) line to incubation with virulent or virulence-attenuated E. histolytica shed some light on this controversy. The data obtained suggest that amoeba interference of the integrin-focal adhesion signaling pathway plays a role in the induction of human cell retraction and death. Using silenced strains, the authors reported that this phenomenon occurred independently of the cytolytic amoebapore but required galactose-inhibitable parasite adhesion, likely involving amoebic Gal/GalNAc lectin, and active E. histolytica CPs . This is in agreement with the finding that secretory CPs of E. histolytica are capable of inducing anoikis in MDA-MB-231 cells. Anoikis is a specialized type of apoptosis that mammalian epithelial, endothelial, and various other cell types experience upon their detachment from the extracellular matrix (ECM). Based on the results of these two studies, amodel of pathogenesis of amoebiasis may be proposed involving CP-mediated degradation of ECMcell and cell-cell attachments, thereby inducing anoikis of hepatocytes and enterocytes,which could lead to liver abscess and colonic ulcer formation. However, further investigations are needed to elucidate the role of anoikis in amoebiasis .
2.1.7. Proteins of the Immune System Are Cleaved by Cysteine Proteases
Immunoglobulins. Secretory IgA (sIgA) Abs are considered a first line of specific defense against natural infections in the vast area occupied by mucosal surfaces . sIgA functions in vivo by reducing mucosal colonization by pathogens and neutralizing diverse toxins and enzymes . E. histolytica elicits a local immune response, in which an increase in specific IgA is detectable in several compartments associated with themucosa [157, 158]. IgA inhibits the in vitro adherence of amoebae to epithelial cell monolayers by recognizing several membrane antigens [157, 159], reduces proteolytic activity , and has amoebicidal action . Several lines of evidence indicate that sIgA protects against E. histolytica infection . Children with stool IgA specific for lectin appear to be protected from intestinal infection , and Gal-lectin heavy subunit-specific intestinal IgA is sufficient to provide immunity against E. histolytica intestinal infection in a baboon model .
Interestingly, several lines of in vitro evidence support that E. histolytica strikes back by using its surface and secreted CPs to degrade host sIgA. When serum and sIgA are exposed to viable axenic trophozoites (strain HM-1:IMSS), a parasite sonicate, or conditioned medium by incubation with live amoebae, sIgA is completely degraded, and proteinase activity is maximal at a neutral pHand is completely inhibited by E-64 . Serum and sIgA are susceptible to degradation by amoeba surface-associated CPs; both sIgA1 and sIgA2 are degraded in a similar fashion by surface amoeba proteases. However, sIgA2 is functionally more resistant to proteolysis than sIgA1 . The CP identity was not determined, but it could be a 70 kDa protease . EhCP4was recently shown to degrade IgA in vitro ; however, it isunclear if it is the same protease or if E. histolytica has more than one IgA-degrading protease. In fact, Garcia-Nieto identified this activity as a surface protease, and EhCP5, unlike EhCP4, is found in the amoeba plasma membrane , leading the authors to suggest that this enzyme could be themain protease involved in sIgA degradation .
Specific antiamoeba IgG responses are developed in >95% of patients with amoebiasis or even in individuals with E. histolytica asymptomatic colonization . However, it is difficult to ascribe a protective role to IgG because the level of Ab response correlates with the length of disease not with the clinical response to infection , and mixed results have been obtained with animal models [165, 166]. This may be because IgG is cleaved by amoebic CPs . A 56 kDa proteinase purified from E. histolytica cleaved polyclonal human and monoclonal murine IgG in a dose-dependent manner. Intact trophozoites also cleave IgG. The resulting cleaved mAb bound to trophozoites with lower affinity than the uncleaved antibody, indicating a decrease in affinity and limiting the effectiveness of the host humoral response to the parasite [11, 167]. The identity of the CP in this study is also unknown, although later in vitro studies with purified recombinant enzymes have demonstrated that EhCP1 , EhCP4  and EhCP5  degrade IgG.
Immunoglobulin cleavage in amoebiasis could decrease the affinity of the antibody for antigen, or if the Fc portion was removed, trophozoites could evade the immune system by coating their surface molecules in Fab fragments . This would prevent the activation of complement by the classical pathway and attack from immune cells bearing corresponding Fc receptors [34, 119].
Complement. Complement (C) is activated when zymogens in serum are cleaved and produces an enzyme cascade that results in nonspecific binding of C components to pathogen surfaces. The two primary functions of C are to directly lyse foreign cells by the membrane attack complex C5b-9 and to opsonize pathogens with C molecules . C3 is the central molecule of the alternative pathway; hydrolysis of C3 generates its active fragments C3a and C3b. Covalent deposition of C3b on nearby surfaces triggers a cascade of events that end in membrane attack complex-mediated lysis .
The interaction of amoebae with C seems to be quite complex. In a comparative in vitro analysis of several amoebic strains, a tight correlation between C resistance and the degree of amoebic virulence was observed . However, during liver abscess development in hypocomplementemic animals, tissue damage and parasite survival increase . E. histolytica can resist lysis mediated by the C system through extracellular C activation through the action of EhCPs. The 56 kDa secreted neutral CP activates the alternative pathway of C by cleaving C3 in the fluid phase. CP action is similar to C-derived C3 convertases, and no further degradation occurs. Therefore, the C3b-like molecule produced is hemolytically active, as demonstrated by its ability to accelerate activation of the alternative pathway of C in rabbit erythrocytes . Only C-sensitive nonpathogenic E. histolytica are lysed by this fluid phase-activated C3blike molecule, while pathogenic strains are resistant . Pathogenic amoebae most likely achieve this by inhibition of surface deposition of the membrane attack complex [119, 172]. The protease involved has not been identified; however, EhCP1 has been shown to cleave C3 in a manner identical to that of the secreted 56 kDa native proteinase  to generate the [alpha]' subunit and possibly forman active C3bmolecule . Likewise, EhCP4 cleaves C3 and produces a fragment with a size similar to that of C3b; however, unlike EhCP1, EhCP4 degrades C3b-like ones . Further studies are needed to determine whether C3 is actually activated in vivo by EhCP1 or by an as-yet unidentified protease or if it is actually degraded by EhCP4.
C3a and C5a, the small cleavage fragments released by C activation, are potent mediators of inflammation and anaphylatoxins . The same extracellular proteinase (56 kDa) of pathogenic E. histolytica is capable of limiting a potential host defense mechanism by degrading C3a and C5a . The anaphylatoxin blockade decreases immune detection in the blood and reduces inflammation in amoebic lesions, partially explaining the lack of severe inflammation in advanced liver and intestinal lesions .
Molecules Involved in Inflammation. In a SCIDmouse-human intestinal xenograft model, infection with E. histolytica trophozoites elicits a robust inflammatory response from the grafted tissue, characterized by strong IL-1[beta] and IL-8 expression, an early neutrophil influx, and extensive damage to the intestinal graft . Trophozoites with reduced CP activity fail to induce intestinal epithelial cell production of the inflammatory cytokines IL-1[beta] and IL-8 and cause significantly less gut inflammation and damage to the intestinal permeability barrier . Purified amoebic CPs possess IL-1[beta] converting enzyme (ICE or caspase-1) activity in vitro, cleaving recombinant human pIL-1[beta] into a biologically active form of IL-1[beta] . These purified proteases are most likely EhCP1 and EhCP2 [21, 22]. In thiswork, the authors postulate that amoebae first bind to intestinal epithelial cells and then lyse those cells through the action of amoebapore [175, 176]. The lysed cells may release pIL-1[beta], which could then be activated by extracellular amoebic CPs with ICE activity and further amplify the inflammatory process in amoebic colitis .
IL-18 is expressed in intestinal epithelial cells  and is a coinducer of theTh1 response. The resulting stimulation of IFN[gamma] then activates macrophages, the major cell capable of killing E. histolytica trophozoites . IL-18 and IL-1[beta] maturation requires cleavage by caspase-1. However, in contrast to the activation of proIL-1[beta] by amoebic lysates, purified rEhCP5, rEhCP1, and rEhCP4 cleave proIL-18 and mature IL-18 to biologically inactive fragments [14, 17, 20]. These contradictory findings, in which EhCPs have opposite effects in two proinflammatory cytokines, have not been explained; the balance between these EhCPs is likely controlled by a complex interplay of parasite and host molecules.
2.1.8. Proteins Containing Iron Are Degraded by Amoebic Proteases for Use as Iron Sources for Growth. Iron is a vital element for the survival of almost all organisms. However, under physiological conditions, [Fe.sup.3+] is not soluble, and [Fe.sup.2+] is soluble but toxic and readily oxidizes to [Fe.sup.3+]. To increase solubility, avoid toxicity, and keep iron away from intruders, this element is normally complexed to proteins; thus, the free iron concentration is far too low to sustain the growth of intruders. However, successful pathogens are able to scavenge iron from host proteins [179-183]. E. histolytica, as well as other amitochondriate protists (e.g., Tritrichomonas, Trichomonas, and Giardia), requires particularly high amounts of extracellular iron in vitro (~100 [micro]M), surpassing that of the majority of both eukaryotic and prokaryotic cells (0.4-4 [micro]M) . This high iron requirement is attributable to the heavy reliance of their energy metabolism on Fe-S proteins [185-187].
We have reported that E. histolytica trophozoites are able to use four of the human iron-containing proteins as iron sources for the parasite's growth in axenic culture medium in which ferric ammonium citrate was substituted by the ferrous- or ferric-protein under investigation. These proteins are hemoglobin, transferrin, lactoferrin, and ferritin . In all cases, amoebae were able to endocytose and cleave the protein to obtain the needed iron (Figure 7). The use of these proteases by trophozoites could be considered a virulence factor because the pathogens seek out host iron to survive in the hostile host environment [179, 182, 183, 188, 189].
Hemoglobin. Hemoglobin (Hb) is a globular protein that is present at high concentrations in erythrocytes or red blood cells (RBCs). The function of Hb is to trap oxygen in the lungs and transport it through the blood to tissues and cells. In adult mammals, Hb is composed of two alpha and two beta chains, each containing one heme prosthetic group; therefore, there are four [Fe.sup.2+] atoms in the Hb molecule, which has Mr of 64.5 kDa . Hb uptake by E. histolytica trophozoites occurs by disrupting the RBC cytoplasmic membrane with surface hemolysins and phospholipases. The major amoebic hemolytic activity has been characterized in rat RBCs; this activity was detected in a vesicular fraction [191, 192]. An alkaline phospholipase has also been associated with virulence .
In E. histolytica, there is little information regarding how iron is obtained from Hb. This parasitic protist is extremely active as a phagocytic cell; once phagocytosed, human RBCs are broken down by amoebae. Chevez et al.  described complete RBC digestion in ~6-8 h by Perl's staining experiments. Quantitative digestion assays using diaminobenzidine staining to visualize RBCs revealed that, after 9 h of RBC phagocytosis, Hb was thoroughly degraded [194-196]. Several researchers have studied the role of amoebic hemoglobinases in the cleavage of different types of Hb. The degradation of native bovine Hb at pH 7.6 by extracted proteinases from different monoxenic strains was observed . Thirty-five years ago, two proteinases against native bovine Hb were purified : one of 41 kDa, with optimal activity at pH3.5, and another of 27 kDa, with optimal activity at pH 6.0. Subsequently, a cytotoxin of 22 kDa with strong proteolytic activity against denatured Hb at an optimal pH of 4.5 was described, and a cathepsin B of 16kDa that was active against native and denatured Hb was purified [24-26]. Perez-Montfort et al.  identified two proteins of 32 and 40 kDa that were able to degrade denatured Hb.
Our group has described three proteases of 21, 82, and 116kDa in extracts of E. histolytica HM-1:IMSS. These proteases were able to degrade human, bovine, and porcine Hbs, mainly at pH 7.0, and were inhibited by PHMB, E-64, NEM, and IA, all of which are specific CP inhibitors . Becker et al.  reported a 30 kDa protease in vacuoles that previously contained phagocytosed RBCs; electrophoretic analysis revealed the incorporation of Hb monomers into trophozoites. In parallel to the decrease in human Hb during RBC digestion, X-ray analysis revealed a loss of iron content [198, 199]. In vitro assays have demonstrated that purified recombinant EhCP112 and EhCP5 are able to degrade Hb [8, 9].
Transferrin. In mammals, iron is mainly transported by transferrin (Tf), a protein found in serum and lymph that delivers iron to all sites, mainly to tissues with active cell division and bonemarrow erythroid cells synthesizing Hb. Tf is part of a family that also contains lactoferrin. Tf has a [K.sub.d] for [Fe.sup.3+] of [10.sup.-22] M and is extremely stable against degradation when saturated. Tf is a glycoprotein of 80 kDa with two lobes, each containing one binding site with differing affinity for [Fe.sup.3+]. TfR1 is a dimeric glycoprotein of approximately 90 kDa per subunit that is expressed in nearly all cells [200, 201].
Interestingly, one of the amoebic receptors for holoTf is the acetaldehyde/alcohol dehydrogenase-2, an enzyme that requires iron. HoloTf is endocytosed through clathrin-coated vesicles and transported to lysosomes, likely losing the first iron in early endosomes and the second in lysosomes due to the acidic pH [28, 202]. To determine whether trophozoites possess cytoplasmic or secreted proteases that can degrade holoTf, total extracts and culture supernatants (SN) of medium with ferric citrate or in the absence of iron were analyzed for holoTf cleavage. Four bands of holoTf degradation corresponding to 130, 43, 20, and 6 kDa were observed in the extracts. In contrast, five bands of 130, 70, 50, 35, and 30 kDa were observed in the SN. All of the proteolytic activities were of the cysteine type. Secreted CPs could play a key role in cleaving Tf when amoebae travel by the portal vein to the liver and when, upon remaining in the liver, produce hepatic abscesses .
Lactoferrin. Lactoferrin (Lf) is a glycoprotein from the innate immune system that is secreted to mucosae; it is abundant in colostrumand milk. Lf is secretedwithout iron (apoLf) by the secondary granules of neutrophils at the infection site; thus, it isamarker of inflammatorybowel diseases (IBDs) . One of the functions of Lf is to chelate iron to make it unavailable to intruders. Lf is a single polypeptide chain that is folded into two lobes; like Tf, each lobe can bind one [Fe.sup.3+]. The degree of Lf glycosylation determines its resistance to proteases and to very acidic conditions. Apo-Lf has a higher avidity for iron than apo-Tf. HoloLf releases iron only in very acidic environments (e.g., pH < 4), and its conformation changes according to the saturation state.When saturated, Lf is more stable and resistant to proteolysis [204-206].
HoloLf can be used as a sole iron source for in vitro growth by E. histolytica trophozoites in a similar fashion to that observed for ferric citrate. HoloLf was recognized by two proteins (45 and 90 kDa) located in the amoebic membrane, and its binding was specific . HoloLf enters the amoeba by a clathrin-independent via (possibly caveolae-like structures). Following endocytosis, holoLf is found in vesicles similar to early endosomes and is then delivered to late endosomes and lysosomes. Delivery of holoLf to lysosomes may be required for its digestion by proteases and iron release, which only occurs in a very acidic milieu. CPs of 250, 100, 40, and 22 kDa from amoebic extracts cleaved holoLf atpH7; however, the activity increased considerably at pH 4 . In acidic lysosomes, the iron from holoLf is likely released, and the protein is degraded by CPs. Culture SN did not contain proteolytic activity against holoLf. Whether E. histolytica contains a reductase capable of changing the iron oxidation state remains unknown. This mechanism seems to be shared by other parasites, such as Tritrichomonas foetus . As amoebae develop in the intestinal mucosa where Lf is found, this protein could be the iron source for the parasite at the beginning of infection, in addition to iron-containing bacterial proteins.
Ferritin. Due to the toxicity of iron, all life forms must have a mechanism to store/scavenge excess iron. Human ferritin is a major cytosolic protein with the capacity to capture up to 4,500 iron atoms. When the intracellular iron level increases, ferritin sequesters iron inside its cavity to detoxify the cell and prevent damage. Ferritin is abundant in the liver, which stores ~50%of the body's total iron reserves. The mammalian ferritin family generally consists in spherical proteins. Each 474 kDa molecule consists of 24 subunits that are either heavy (H) or light (L) with a molecular mass of ~21 and ~19 kDa, respectively [208-211].
Ferritin uptake by amoeba may be mediated by a binding protein because it is concentration and time dependent, highly specific, and saturable at 46 nM ferritin. E. histolytica can cleave ferritin into several fragments. Three neutral CPs (100, 75, and 50 kDa) were observed to degrade ferritin in culture extracts. Ferritin entrance is constrained by inhibitors of clathrin-coated pits, and after 30min of incubation, ferritin colocalized with an anti-rat LAMP-2 Ab in lysosomes . The liver invasion by E. histolytica is poorly understood. Once liver cells are destroyed by amoebic enzymes, ferritin can be released and may be endocytosed by trophozoites and used as a source of iron and nutrients to form hepatic abscesses. In the liver, amoebae may also use Hb as an iron source; however, ferritin can provide up to 1,000-fold more iron than Hb. The capacity of E. histolytica to utilize ferritin as iron source may well explain its high pathogenic potential in the liver.
3. Entamoeba histolytica Related Strains
Virulence is a complex phenomenon that depends on two general properties: the invasiveness, or ability of microorganisms tomultiply and to cause localized tissue destruction, and toxigenicity, or the ability to produce and secrete substances that can cause distant lesions. However, the virulence of E. histolytica related strains likely depends mainly on the tissue-damaging potential of individual trophozoites and the number of invasive amoebae in the infected host .
The role of amoebic proteases as responsible for tissue destruction in amoebiasis has been discussed in the previous section [7, 13, 91]. Amoebic proteases have been shown to degrade several tissue components such as collagen, fibronectin, and laminin [13, 91]. The pathogenicity and degree of virulence of various amoebic strains are determined not only by their level of protease activity but also by the nature of their corresponding enzyme proteins. The most studied proteases from E. histolytica related strains are summarized in Table 2.
3.1. Entamoeba moshkovskii or Entamoeba histolytica Laredo Type. The Entamoeba histolytica Laredo strain was isolated 50 years ago from a resident of Laredo (USA) who suffered from diarrhea, weight loss, and epigastric pain . Further molecular studies and biological features revealed that the E. histolytica Laredo strain is identical to Entamoeba moshkovskii , a species with a worldwide distribution that is considered a nonpathogenic free-living amoeba. Recently, some studies have reported the possibility that E. moshkovskii could be a pathogenic species with the capacity to infect humans or be associated with gastrointestinal symptoms .
E. moshkovskii is frequently found in regions where amoebiasis is highly prevalent. It has been isolated from wastewater, freshwater from rivers and lakes, brackish water, and human feces samples; this last finding would seem to suggest that E.moshkovskii could be pathogenic. Both the Laredo strain and E. moshkovskii grow at room temperature as well as at body temperature (37[degrees]C). They present trophozoite and cystic forms, and their size varies depending on the strain. In culture, trophozoites are able to complete cycles of division, encystation, and excystation. Usually one to four nuclei are observed, but there are forms withmore nuclei, which tend to occupy a central position in the mature cysts [213, 215, 216].
When observed by light microscopy, E. moshkovskii does not have morphological features that permit its distinction from E. histolytica and E. dispar . E. histolytica can grow at temperatures ranging from 27 to 36.5[degrees]C, while E. moshkovskii can grow at temperatures from 4 to 40[degrees]C and in the presence of low amounts of nutrients that are not suitable for the growth of other Entamoeba species. E. moshkovskii can also adapt to extremely hypotonic cultures as it develops a contractile vacuole, a characteristic that is not present under normal growth conditions . An assay was recently reported for the differential detection of E. histolytica, E. dispar and E.moshkovskii. This method consists of a single PCR step and is highly sensitive and potentially quantitative. It is possible to detect 0.2 pg E. histolytica and 2 pg E. dispar or E. moshkovskii DNA .
Homogenates of the Laredo strain or E. moshkovskii were tested against RBCs from different mammalian species. The homogenates had significant activity against hamster RBCs, but human RBCs were highly resistant to lysis . A comparison of the acid and neutral protease activities of the Laredo strain with other, less virulent strains revealed that the Laredo strain had significantly less activity . This strain was tested for its capacity to digest native radioactive type-I collagen gels and produce liver abscesses in newborn hamsters. Laredo strain did not show collagenolytic activity and failed to produce lesions. Thus, the susceptibility to invasive infection may depend on the characteristics of the extracellular components of host tissues and the potential virulence of the parasites . Additionally, this amoeba was unable to produce secreted electron-dense granules, which are associated with an increase in collagenolytic activity in E. histolytica . Amoebic proteases and host leukocytes were studied in a model of acute experimental amoebiasis that was produced by the intratesticular injection of axenic trophozoites in rats. The degree of inflammation and necrosis produced by E. moshkovskii was indistinguishable from the control without lesions, and this was correlated with the protease activity measured against azocasein. In this case, the protease patterns of E. histolytica type Laredo and E. moshkovskii were different because E. histolytica type Laredo displayed high protease activity and caused minimal tissue damage.
The current evidence proposes that the risk factors for acquiring infections by E. moshkovskii are similar to those described for E. histolytica and E. dispar. However, the capacity of E. moshkovskii to grow in the environment and adapt to adverse conditions suggests that the risk of infection is even greater than that for E. histolytica or E. dispar infection [213, 215, 218].
3.2. Entamoeba dispar. E. dispar is a nonpathogenic (commensal) amoeba with a morphology, genetic background, cell biology, and host range similar to that of E. histolytica. Together, E. histolytica and E. dispar infect approximately 10% of the world's population, but E. dispar is much more common. Studies of isoenzymatic patterns, antigenicity, and genetics have led the WHO to declare that E. histolytica and E. dispar should be classified as distinct species based on genetic differences inmultiple genes [133, 219]. Recently, E. dispar was reported to present pathogenic behavior depending on the strain and culture conditions . E. histolytica equivalent genes for virulence have been detected in E. dispar. The importance of CPs in the pathogenesis of invasive amoebiasis is indisputable; CPs are important virulence factors and the main proteolytic enzymes in E. histolytica [34, 133].
In the beginning of the 1990s, studies of E. dispar strains grown in xenic cultures revealed important differences in the proteolytic activity on albumin and gelatin compared to E. histolytica axenic and xenic cultures . As discussed earlier, within the total sequence of the E. histolytica genome, more than 40 genes encoding CPs have been identified [71, 73, 222]; only 8 of these CPs are moderately expressed in culture . The majority of the protease activity detected in E. histolytica lysates is the result of the ehcp1, ehcp2, and ehcp5 genes. E. dispar encodes 4 genes (edcp1 to edcp4) homologous to those of E. histolytica [73, 133]. Functional genes homologous to ehcp1 and ehcp5 are absent in this nonpathogenic species [35, 92].
The characterization of proteases in E. dispar has revealed important insights for the understanding of invasive amoebiasis [34, 133]. CP genes that are associated with virulence were thought to be expressed in E. histolytica but not E. dispar, such as acp1 (ehcp3) ; a related homologue with 95% identity was identified in E. dispar (edcp3) [84, 223]. The same phenomenon was observed for the ehcp2 gene, which was subsequently detected in clinical strains of E. dispar [35, 73, 77]. The strategy was changed, and E. histolytica proteases were expressed in E. dispar; EhCP2 was overexpressed in E. dispar by episomal transfection, yielding almost the same amount of total CP activity as E. histolytica. EhCP2 overexpression produced increased monolayer destruction in cultured mammalian CHO cells but was unable to produce liver abscesses in an animal model. The same result was observed when E. dispar wasmodified to overexpress EhCP5, a CP that is not present in this amoeba, in vitro [71, 149]. EhCP5 activity increased almost 3.0-fold, leading to greater monolayer destruction .
In an effort to characterize the molecular basis for the failure to express a CP5-analogous enzyme in E. dispar, the ehcp5-containing genomic regions from E. histolytica and E. dispar were compared. The gene corresponding to ehcp5 is present and the location is conserved in E. dispar, but the gene is highly degenerate and does not contain any open reading frame, suggesting that the gene has been nonfunctional for a considerable period of time during the evolution of the nonpathogenic amoeba species . Degeneration of the edcp5 gene constitutes a loss of a specific and functional accessory molecule important for processing CP5 [87, 92, 224]. Recent advances in DNA-mediated gene transfer in E. histolyticamay help to prove or disprove this hypothesis.
In comparison with E. histolytica, the expression of CPs in E. dispar is lower (CP activity in E. histolytica is 10-1000-fold higher than in E. dispar), which could explain the differences in pathogenicity between these species [35, 73]. However, it is not clear if the ability of E. histolytica to invade is due to the synthesis of more CPs or because these enzymes differ in activity . Thus, the systematic comparison of E. histolytica and E. dispar constitutes an important area of research to identify and analyze factors thatmay be important for amoebic pathogenicity.
After phagocytosis, cytoplasmic (EhCP3) and membrane-associated (EhCP2) proteases are released into phagocytic vesicles in E. histolytica and E. dispar . A comparative analysis of maturation of phagolysosomes by acidification, recruitment of hydrolases, and degradation of phagosomal content between the two species was performed; this sequence of events is crucial for the ability to ingest and kill microorganisms and host cells. Phagosomes of E. histolytica are much larger and contain a greater number of bacteria than those of E. dispar and have more efficient acidification. These differences reflect the nature of proton pumping across the phagosomal membrane and membrane trafficking, leading to phagosome maturation. Acidification is essential for the activation of hydrolytic enzymes and degradative proteins such as EhCP1, EhCP2, EhCP4, and EhCP5, phospholipases, and lysozyme. Degradation in E. histolytica phagosomes occurs more rapidly than in E. dispar phagosomes, in agreement with the previous findings that CP activity in E. histolytica is 10-1000-fold higher than in E. dispar and that specific inhibitors do not prevent degradation in E. dispar phagosomes, possibly because the CPs from E. dispar are relatively more resistant to inhibitors or these enzymes are not essential for digestion in phagosomes. These differences in phagosome degradation and the enzymes involved in host cell degradation could determine the difference in the outcome of infection with these two species .
Recently, the capacity of the E. dispar ICB-ADO strain to cause liver damage and destroy cell culture lines in the presence of common intestinal bacteria was reported. This amoeba, which was isolated from a patient who was symptomatic but not dysenteric, was able to induce liver necrosis in hamsters in an even more severe form than that produced by E. histolytica and exhibited high CP activity . Thus, E. dispar could exhibit pathogenic behavior with the potential to have an invasive role, depending on the strain and host conditions. The authors also discussed whether E. dispar is a commensal or pathogenic parasite; they present evidence that bacteria are able to contribute to the regulation of factors such as proteases, which could have an important impact on E. dispar virulence. Liver lesions with a large number of amoebae are in close contact with hepatocytes and induce an inflammatory reaction, ultimately resulting in an extensive necrotic area with a limited number of trophozoites compared to E. histolytica HM-1:IMSS. By contrast, the damage produced to MDCK cells was less extensive. Accordingly, the proteolytic activity of an E. dispar polyxenic strain was increased by almost 75% in comparison with a monoxenic strain and by 50% in comparison with E. histolytica .
Research on E. histolytica and E. dispar has primarily focused on the large family of CPs, which has been firmly linked to amoebic virulence, but numerous proteases are encoded in the E. histolytica genome. Only one copy of the gene is present in E. dispar; this gene is most closely related to ehmsp-2. The ehmsp-1 gene functions in the regulation of adherence and also affects motility, tissue culture monolayer destruction, and phagocytosis, as described previously .
3.3. Entamoeba invadens. E. invadens is a protozoan that causes invasive disease in reptiles and has the same two stages of life cycle and pathogenic potential as E. histolytica. The trophozoite is the stage that lives within the infected host, while the transmissible stage is the quadrinucleated cyst. In some of its hosts, E. invadens causes liver and intestinal damage with similar pathology to that of E. histolytica-infected individuals. This amoeba is studied as a model of encystation and excystation in the Entamoeba genera since E. histolytica in vitro encystation has not been achieved [227, 228]. Proteases and, in particular, CPs are important for several cellular processes such as differentiation or host cell invasion. In contrast to E. histolytica, very little is known about the E. invadens genome .
To study proteases and their role in virulence in Entamoeba spp., amoebic cytopathogenicity was compared among several axenized strains. Each amoeba species possesses distinctive virulence, defined by the rate of tissue destruction, which, in turn, can be correlated with the presence or absence of hydrolases. To identify specific cytopathic effects, hemolytic profiles of homogenates of two E. invadens strains were compared in RBCs of different origins. The E. invadens strain PZ has no activity on RBCs, while the strain IP-1 has very high specific hemolytic activity against hamster and mouse RBCs . A subsequent study revealed that the enzymatic activity is similar in E. histolytica and E. invadens. Proteases from these species were stable under denatured conditions and were inhibited efficiently by NEM and IA, indicating that they are CPs . The importance of E. invadens CPs became apparent upon the observation that the major CP degrades azocasein at optimal pH of 4.8 and is similar to the cathepsin B-like CP of E. histolytica. In addition, E. invadens proteases are activated by DTT and inhibited by typical CP inhibitors (E-64, IA, and PHMB). Further studies demonstrated that CPs may be critical for the survival of E. invadens as the specific inhibition of these proteases may ultimately interrupt parasite transmission . Interestingly, in the presence of SDS, the E. invadens CPs seem to be processed for autoproteolysis .
A model of acute experimental amoebiasis in rat testis was used to study the role of proteases. In this work, the severity of testicular lesions was correlated with the level of protease activity observed in E. histolytica strains and in E. moshkovskii. The exceptionwas E. invadens, which displayed high protease activity but produced minimal testicular damage, suggesting that both the pathogenicity and degree of virulence of various amoebic strains are determined by the level of protease activity and by the type of enzyme . Several proteaseswith homology to the ehcp3 and ehcp2 genes of E. histolytica have since been reported in E. invadens. Purified E. invadens CPs exhibit substrate specificity similar to that of E. histolytica CPs and are inhibited by specific inhibitors. E. invadens possesses CPs, MPs, and SPs. CPs clearly play a role in encystation because the use of inhibitors reduces the degree of encystation, but this effect seems to be produced by decreased amoebic viability . In addition to CPs, other proteins that are differentially expressed during encystation have been described, such as chitinases that are directed to the cell surface inside small secretory vesicles [230, 231].
Proteases are involved in the differentiation process in E. invadens. The proteasome system is very important during the conversion of trophozoites into infectious cysts, as evidenced by the disruption of the encystation process by proteasome inhibitors, while CPs inhibitors only delay the process . In the cyst, the most abundant proteases are serine-type proteases, which could be involved in the synthesis of chitin, most likely by activating the chitinsynthase proenzyme, because AEBSF, a specific inhibitor of SPs, prevented the formation of viable mature cysts. Thus, life cycle and transmission can be interrupted by specific inhibitors of SPs . Therefore, both chitinase and SPs are essential for cyst wall destruction during the excystation process . The participation of CPs in encystation and excystation of E. invadens was confirmed by using E-64 .
Presently, several CP genes have been identified in E. invadens IP-1 based on their homology with genes in E. histolytica (eicp-a3, -a5, -a9, -a11, -b7, -b9, -b10, and -c2). Twenty of these genes are expressed during axenic parasite cultivation, whereas the remaining are not expressed or expressed at very low levels. The expression patterns of eight of the identified E. invadens CP genes were evaluated during the encystation or excystation processes. eicp-b9 is the major gene expressed during encystation that is not involved in Entamoeba autophagy, but its specific function during encystation is unknown . The MPs encoded by ehmsp-1 and ehmsp-2 in Leishmaniamajor were obtained in E. histolytica and E. dispar but were absent in E. invadens. This implies that ehmsp-1 and ehmsp-2 are the result of gene duplication just previous to the divergence of E. histolytica and E. invadens. Thus, E. histolytica is more closely related to E. dispar than to E. invadens, but in some time, E. dispar had both gene copies before losing edmsp-1 .
4. Pathogenic Free-Living Amoeba Species
Free-living amoebae are protist organisms distributed worldwide. A small number of species have been implicated in human disease: Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris. Some of these infections are opportunistic and mainly occur in immunocompromised hosts, while others affect healthy people only under certain conditions. Although the number of infections caused by these protozoa is low, research is needed due to the difficulty in diagnosing these diseases and the lack of specific treatments, which results in high mortality, mainly by encephalitis. Unfortunately, molecular studies of free-living amoebae have not been performed. However, some virulence factors of these parasites have been purified and characterized. The most studied proteases from pathogenic free-living amoeba species are summarized in Table 3.
4.1. Acanthamoeba spp. Acanthamoeba is the most common free-living amoeba genus and is ubiquitously distributed. Acanthamoeba species are opportunistic amphizoic protozoa that are found in soil, air, and water, although they have also been isolated from vegetables and some animals. These amoebae cause several diseases in humans, such as granulomatous amoebic encephalitis (GAE) in immunecompromised patients and keratitis in contact lens wearers. Protozoa are resistant to diverse environments because they can tolerate a wide range of osmolarity, salinity, pH, and temperature. Acanthamoeba species that cause human infections are A. castellanii, A. polyphaga, A. culbertsoni, A. hatchetti, A. healyi, A. astronyxis, A. lugdunensis and A. divionensis. All of these species have a simple life cycle with two phases, a vegetative stage or trophozoite (8-40 [micro]m) and a resistant stage or cyst (8-29 [micro]m). The name Acanthamoeba comes fromthe presence of fine spine-like structures or acanthopodia projecting outward from the surface of the body .
Typical forms of infection by Acanthamoeba spp. Are GAE and nasopharyngeal or cutaneous invasion; amoebae spread by the hematogenous via. GAE is rather rare; approximately 150 cases have been described worldwide. Due to the difficulty in diagnosis, it is possible that other cases of GAE have been misdiagnosed. By contrast, more than 30 cases of keratitis due to Acanthamoeba were reported in Chicago (USA) alone. As of August 2006, more than 5000 cases of keratitis due to Acanthamoeba are estimated to have occurred in the United States, but the actual number of infections around the world is unknown. Large numbers of cases have also been reported in the United Kingdomand India .
The adherence of Acanthamoeba trophozoites to target cells or tissues is an important step in host invasion. Amoebae can adhere to human and animal corneal epithelial cells. Omana-Molina et al.  reported the early adhesion steps of A. castellanii and A. polyphaga trophozoites to hamster cornea. After adherence to the epithelial surface, the trophozoites form clumps and migrate to cell borders, causing separation of adjacent cells and cytopathic damage in distal cells. Amoebic adhesion may be mediated by a 130 kDa mannose-binding protein (MBP), which is a surface-expressed protein . Other adhesins include a laminin-binding protein of 28 kDa  and a 55kDa protein that binds to the laminin of the pathogenic strain A. culbertsoni . Interestingly, A. polyphaga binds to the ECM proteins collagen type IV, laminin, and fibronectin , and calcium enhances this binding . In these interactions, amoebae exhibited a strong attachment to the basal membrane components laminin and collagen IV. The adherence to these molecules leads to secondary responses such as phagocytosis and toxin production that result in host cell death via the phosphatidylinositol 3-kinase (PI3-K) pathway .
In Acanthamoeba, several groups have shown that adherence to the corneal epithelium results in the production of diverse proteases [41, 241]. The action of the proteases includes damage to the collagen shield and degradation of glycoproteins such as plasminogen, fibrinogen, laminin, and hemoglobin . In addition, Acanthamoeba species display plasminogen activator activity, which can trigger host matrix MPs, leading to degradation of basement membranes. In another study, Alizadeh et al.  demonstrated that the addition of mannosylated proteins to the cornea of Chinese hamsters induced the expression of a protease of 133 kDa (MIP133) that mediated apoptosis of corneal epithelial cells, facilitated corneal invasion, and degraded the corneal stroma. Acanthamoeba spp. also possess hydrolytic enzymes, such as elastases , phospho-lipases , SPs [47, 244-247], CPs [47, 244-247], and contact MPs .
In the last twelve years, several proteases from distinct species of A. polyphaga have been purified and characterized. Proteases have been obtained from both total crude extracts and conditioned culture medium and tested with natural and synthetic substrates. Alfieri et al.  utilized gelatin-containing gels and azocasein to demonstrate azocasein hydrolysis by cell lysates at an optimal pH of 4.0-5.0; this hydrolysis was predominantly associated with CP activity. By contrast, culture SN contained significant azocasein hydrolyzing activity corresponding to SPs. We  performed a partial biochemical characterization of proteases in total crude extracts and conditioned culture medium from A. castellanii and A. polyphaga strains by using gelatin-containing gels and azocoll assays (both denatured type-I collagen). Interestingly, 17 proteolytic bands distributed between both Acanthamoeba strains were observed. The Mr of these bands ranged from 30 to 180 kDa in A. castellanii and 34 to 144 kDa in A. polyphaga. Incubation with protease inhibitors revealed that the proteolytic activities mainly belonged to the SP type, followed by CPs, in both total crude extracts and conditioned culture medium.
Numerous proteases in Acanthamoeba are able to degrade ECM components. He  described the presence of a collagenolytic enzyme from A. castellanii culture medium that partially digested collagen shields after 4 h of incubation, with complete degradation by 8 h. Similar results were obtained with shields incubated in purified collagenase solutions. More importantly, when naive Lewis rats were treated with Acanthamoeba-conditioned cultured medium, corneal lesions were produced that were clinically similar to those found in biopsy specimens of human patients diagnosed with acanthamoebic keratitis. The use of nonspecific protease inhibitors and EDTA-Na in the Acanthamoeba-cultured medium completely blocked the degradation of collagen shields, and the use of EDTA-Na in vivo also blocked amoebic collagenase activity. The authors demonstrated that the parasite-derived culture medium most likely contained considerable amounts of collagenase and low concentrations of other proteolytic enzymes. Thus, the authors speculated that, in the initial infection, Acanthamoeba-derived collagenase acts as a priming agent to produce collagenolysis, edema, and neutrophils infiltration in human keratitis.
Excretory and secretory products of A. polyphaga culture have been described by , who observed major bands of proteolytic activity in nondenatured gelatin substrate gels with Mr of 36, 49, and 66 kDa in homogenate and excreted/secreted products. These proteases showed a wide pH range of activity, with an optimum at pHs 7-9. The authors also described the collagenolytic activity of A. polyphaga culture medium on the substrates azocoll and gelatin and native type-I collagen. They concluded that A. polyphaga secretes multiple SPs, CPs, and MPs and that all of these proteases contribute to the collagenolytic effect. Recently, Ferreira et al.  characterized elastase activities in the culture medium of A. polyphaga. These activities are in the range of 70-130 kDa, with an optimal pH of 7.5; in addition, these activities are inhibited by PMSF, antipain, chymostatin, and 1,10-phenanthroline and partially reduced by elastinal and EDTA. This study demonstrated that amoebic trophozoites secrete elastase activities and suggested high-molecular-weight SPs as possible elastase candidates.
A secreted SP of A. healyi was purified by Kong et al. . The protease has a molecular weight of 33 kDa and an optimum pH of 8.0; interestingly, the optimum temperature was 40[degrees]C. This protease degrades type-I and -IV collagen and fibronectin. The protease activity is inhibited by PMSF and DIFP, both SP inhibitors. Na  purified a secreted SP from A. castellanii with an approximate Mr of 12 kDa. This molecule is a chymotrypsin-like protease that can degrade various substrates, such as collagen, fibronectin, laminin, sIgA, IgG, plasminogen, fibrinogen, hemoglobin, and rabbit corneal protein. The purified protein was also used to test cytopathogenicity toward HEp2 cells, which resulted in a loss of viability within 12 h. The cytopathogenic events were completely inhibited when the protease was pretreated with PMSF before addition to the HEp2 cells.
A 33kDa SP secreted by A. lugdunensis was purified by Kim et al. . The protease showed a pH optimum of 8.5 and a temperature optimum of 37[degrees]C. This protease is able to degrade collagen types I and IV, fibronectin, fibrinogen, hemoglobin, albumin, IgG, and IgA. PMSF inhibited nearly all of the protease activity. Also, the same authors  reported that this 33 kDa protease could be purified from other Acanthamoeba strains with different degrees of virulence.
Sissons et al.  identified two proteases of 130 and 150 kDa from an Acanthamoeba isolate capable of inducing GAE. The 130 kDa protease was inhibited by PMSF, suggesting that it is an SP, whereas the 150kDa protease was inhibited by 1,10-phenanthroline, suggesting that it is an MP. Both proteases exhibited maximal activity at neutral pH and over a range of temperatures. These proteases degrade ECM components such as collagen types I and III, elastin, and plasminogen, as well as casein and hemoglobin. Finally,  described the partial biochemical characterization of proteolytic enzymes secreted by Acanthamoeba spp. trophozoites isolated from the corneal tissues of different patients. Different enzymatic patterns of proteases were observed that varied between single and multiple protease activities. Low-molecular-weight SPs were secreted by trophozoites and were associated with a more severe clinical course of keratitis. Protein extracts of Acanthamoeba trophozoites were assayed for specific activity against type-I collagen. Collagenases were observed in amoebic extracts of different patients. These isolates produced a single band of approximately 36 kDa. All of these patients suffered from severe infections. Consequently, Acanthamoeba proteolytic enzymes could play a role in the degree of virulence and clinical manifestations of disease in human keratitis.
Pathogenic Acanthamoeba species are also known to infect the central nervous system (CNS), resulting in fatal GAE disease. The pathophysiological complications of this disease include induction of proinflammatory responses, invasion of the blood-brain barrier and connective tissue, and neuronal damage. Intranasal, intrapulmonary, and intracardiac inoculations of trophozoites lead to invasion of the CNS, suggesting that amoebae can enter by different routes, including the blood-brain barrier . Several lines of evidence suggest that Acanthamoeba entry into the CNSmost likely occurs at the cerebral capillary endothelium, including the observation that lesions are more frequent in the brain parenchyma of GAE lesions [253-255]. Some studies have focused on Acanthamoeba interactions with primary human brain microvascular endothelial cells (HBMECs) and have demonstrated that amoebae produce HMBEC dysfunction . Proteases appear to play an important role in weakened tight junctions in HBMECs cultures . Acanthamoeba protease-mediated disruption of HBMECs can be inhibited by the SP-inhibitor PMSF, implicating SPs in blood-brain-barrier perturbation [45, 257]. Furthermore, recent studies have shown that Acanthamoeba proteases target ZO-1 and occludin proteins . More studies are needed to comprehend the importance of Acanthamoeba spp. proteases in the diseases that caused by these species.
4.2. Naegleria spp. Naegleria spp. are amoeboflagellates that are foundworldwide in warmfreshwater and that feed mostly on bacteria. These amoebae transform from trophozoites to the flagellate form if nutrients are limited in order to move toward other rich nutrient niches. Amoebae can also transform into cysts to survive adverse conditions. Species of Naegleria have been known for over a century , but it was only approximately 40 years ago that one species, called Naegleria fowleri, was found to cause primary amoebic meningoencephalitis (PAM) in humans . There is a strong indication that the pathogenic N. fowleri evolved from the nonpathogenic species N. lovaniensis . Only 235 PAM cases have been reported worldwide, so the disease is rare. PAM is an acute, fulminant, and often fatal disease that occurs mainly in apparently healthy children or young people with a recent history of swimming, among which only around 5% of patients survive . Infection is acquired by exposure towater in ponds, pools, or lakes contaminated with N. fowleri [259, 260].
Other researchers and our group have found that N. fowleri trophozoites gain access to the CNS by crossing the olfactory bulbs in experimental animals [261, 262]. Once in the CNS, amoebae divide rapidly causing inflammation associated with tissue destruction, leading to death in a few days. The mechanisms involved in the tissue invasion and destruction are poorly understood. However, various in vitro studies suggest the presence of numerous virulence factors. These factors include adhesins , pore-forming proteins [264, 265], phospholipases , contact-dependent lysis , elastase , and secreted proteolytic enzymes with cytopathic effects [48, 50].
There are few reports concerning the adherence of N. fowleri to ECM proteins. An integrin-likemolecule that binds to immobilized fibronectin has been reported . This protein was described as an [alpha]-integrin subunit with a role in cytotoxicity. Shibayama et al.  described the interaction of N. fowleri with human type-I collagen. Recently, adhesion to collagen and fibronectin by the pathogenic strain N. fowleri and the nonpathogenic N. lovaniensis was compared, revealing greater adherence of N. fowleri to fibronectin [269, 270]; N. fowleri presents higher levels of surface glycoconjugates that contain [alpha]-D-glucose and terminal [alpha]-L-fucose residues than N. gruberi. Cytosolic and membrane glycoconjugates were more highly expressed in N. fowleri than in N. gruberi. These differences could be related to the adherence to different substrates, and therefore they could also be related to the pathogenesis of N. fowleri.
Aldape et al.  partially purified a secreted protease of 30 kDa consisting of two proteins from N. fowleri. The biochemical properties of the two forms of N. fowleri protease activity were indistinguishable, suggesting that they may be posttranslationally modified isoforms of the same gene product. This activity was abolished by E-64 and leupeptin, both CP inhibitors. Trophozoites or secreted protease activities were able to degrade mainly collagen and elastin; this effect was inhibited by Z-FA-FMK, a specific CP inhibitor. We have described proteolytic activities from N. fowleri and N. gruberi that are able to degrade azure and azocoll at 37[degrees]C. These activities were mainly inhibited by the CP inhibitors PHMB and E-64, which indicates that the main protease activities in N. fowleri are thiol-proteases, although there are also lesser quantities of SP activity . More studies are needed to elucidatewhether specific proteases fromN. fowleri can degrade ECM proteins such as type-I and -IV collagen, fibronectin, elastin, and laminin.
We recently evaluated the role of mucins in the natural immune response and the role of the mucinolytic activity of trophozoites of N. fowleri . A CP with Mr of 37 kDa with mucinolytic activity was identified. This protease could be an important molecule in mucin degradation and evasion of the host innate immune response. The study of Naegleria virulence factors is still limited; therefore, much future work is needed, particularly with respect to the role of proteases in the invasion of the CNS.
4.3. Balamuthia mandrillaris. Balamuthia mandrillaris is the causative agent of Balamuthia granulomatous amoebic encephalitis, a life-threatening brain infection . First isolated from the brain tissue of a mandrill baboon , B. mandrillaris is naturally found in soil  and occasionally invades humans to cause an insidious infection that can occur in either immunocompetent or immunosuppressed patients. To date, there have been approximately 120 reported cases of Balamuthia encephalitis worldwide, with only two survivors . The incubation period is long and usually results in drastic neurological damage that includes single and multiple space-occupying brain lesions. The amoebic entry routes may be the nasal mucosa or skin injuries resulting from traumas . Studies in mouse models have shown that B. mandrillaris is able to migrate through the olfactory nerve pathway and access the brain in this form [276, 277]. Until now, there have been few in vitro studies in B. mandrillaris. This amoeba has cytotoxic activity against monkey kidney cells [278, 279] and HBMECs , but the cellular mechanisms of cytopathogenesis are unknown. Rocha-Azevedo et al.  described the specific binding of B. mandrillaris to three ECM proteins in vitro: collagen-I, fibronectin, and laminin-1. Binding of amoeba to laminin was greater than that to collagen and fibronectin. The authors found that binding was inhibited when the amoebae were pretreated with sialic acid.
There is little information about protease activities in B. mandrillaris. Matin et al.  used isolates of B. mandrillaris (from human and baboon) to describe protease activities in zymographic assays that revealed major protease bands with approximate Mr of 40-50 kDa. The protease bands were inhibited by 1,10-phenantroline, suggesting that these activities belong to the MP group. These activities were observed over a pH range of 5-11, with maximum activity at neutral pH and 42[degrees]C. The B. mandrillaris proteases could degrade type-I and -III collagen, elastin, and plasminogen. The authors also demonstrated that these proteases do not participate in the cytotoxic effects on HBMECs.
A complete understanding of the pathogenic molecular mechanisms of this disease is needed and may lead to the identification of potential targets for therapeutic management and an accurate diagnosis.
5. Concluding Remarks
The occurrence of tissue damage during infection is a complex phenomenon that is mediated by virulence factors from the parasite as well as exacerbated responses from the host. The numbers of amoebic surface and secreted proteases suggest that they are intended not only for nutrient acquisition but also as virulence factors. It is well established that there is a correlation between virulence and the level of protease expression in virulent amoebae, such as E. histolytica orAcanthamoeba, butnot inother species suchas E. invadens, for which the quality and not the quantity of expression could be important. This could also be true for proteases of other species previously considered nonpathogenic amoebae, such as E. dispar. Parasite tissue destruction may require the participation of many types of protozoan parasite proteases: CPs, SPs, and MPs. The predominant type of protease varies depending on the species; for example, E. histolytica uses mostly CPs, while Acanthamoeba spp. prefers SPs.
There are a great variety of protease targets such asmucin, ECM components, tight junction proteins, immunoglobulins, complement, and cytokines. Iron-containing proteins can also be included as protease targets because iron scavenger capacity is currently considered a virulence factor for pathogens; pathogensmust overcome hostmultiple strategies that limit iron availability as an innate host defense mechanism. In addition, some proteases have a role in adhesion or encystation, which are essential for establishing and transmitting infection. Thus, the lack of proteolytic function of some proteases demonstrates the multipurpose potential of these valuable virulence factors.
Amoebic proteases appear to be nonspecific because they degrade a wide variety of substrates in vitro; however, to correctly assess the role of each protease in infection, it is necessary to evaluate parasite protease activities with models, in vitro models of complex substrates such as 3D synthetic ECM, and ex vivo assays to test the proteolytic activity under conditions similar to that of anactual infection. The participation of host cells and bacteria in the invasion of parasites is also relevant because these cells can greatly influence the amoeba protease profile, as has been reported for E. histolytica and E. dispar, the protease profiles which change extensively after contact with host cells or bacteria. Although a lot of valuable information has been obtained studying axenically grown trophozoites, this information should be carefully extrapolated to what occurs during infection.
Previously, it was thought that amoebic proteases were not able to exert cytopathic damage to cells; however, exciting new evidence suggests that, by degrading the anchorage points of host cells such as the ECM, E. histolytica proteases lead to a special type of apoptosis called anoikis in host cells. In addition, in Acanthamoeba, a protease has been described that mediates apoptosis of corneal epithelium cells.
For many of these parasites, the identities of the proteases are unknown because the reports refer only to proteolytic activities of certain molecular mass ranges, with the exception of the extensively studied E. histolytica. Therefore, it is important to identify each gene responsible for these proteolytic activities to obtain a better understanding of parasite pathogenesis. Once the gene has been identified, it is important to use parasites in which protease genes are deleted or overexpressed. Such studies will be of great value to elucidate the actual roles of parasite proteases as virulence factors. This type of study has only been performed in E. histolytica and E. dispar, with restrictions. In E. histolytica, the only available strategy requires the use of the G3 strain in which the amoebapore is silenced as a genetic background to perform the knockdown of the protease gene. This strategy has the limitation that a protease is assessed as a virulence factor with an already attenuated trophozoite. In addition, silencing strategies have proven to have some degree of nonspecificity, so it has been difficult to assign a function to a specific protease.
Finally, most patients suffering from amoebiasis express antibodies to trophozoite CP, making these proteases an attractive potential vaccine target or a potential tool to improve the early diagnosis of human parasitic diseases. Because transmission, invasion, and life cycle could be interrupted by using specific parasite proteases inhibitors, the study of proteases and their specific inhibitors is relevant to the search for newtherapeutic targets or to increase the power of drugs currently used to treat these diseases, such as in the reduction of tissue damage when specific inhibitors are used in animal models.
Abbreviations of Inhibitors and Activators of Proteases AEBSF: 4-(2-Aminoethyl) benzenesulfonyl fluoride DIFP: Diisopropyl fluorophosphates DTT: Dithiothreitol EDTA: Ethylenediaminetetraacetic acid E64: Trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane IA: Iodoacetamide NEM: N-ethylmaleimide PHMB: p-Hydroxymercuribenzoate PMSF: Phenylmethylsulfonylfluoride TLCK: Tosyl-lysyl-chloromethylketone TPCK: 1-Chloro-3-tosylamido-4-phenyl-2-butanone Z-FA-FMK: Benzyloxycarbonyl-Phe-Ala-fluoromethyl ketone.
J. Serrano-Luna and C. Pina-Vazquez are contributed equally to this paper.
Conflict of Interests
The authors declare that they have no conflict of interests.
The authors thank CONACyT, Mexico, for supporting this work, Projects no. 60102 and 179251.
 J. Pawlowski and F. Burki, "Untangling the phylogeny of amoeboid protists," Journal of Eukaryotic Microbiology, vol. 56, no. 1, pp. 16-25, 2009.
 A. J. Roger and L. A. Hug, "The origin and diversification of eukaryotes: problems with molecular phylogenetics and molecular clock estimation," Philosophical Transactions of the Royal Society B, vol. 361, no. 1470, pp. 1039-1054, 2006.
 T. F. H. G. Jackson, "Entamoeba histolytica and Entamoeba dispar are distinct species; clinical, epidemiological and serological evidence," International Journal for Parasitology, vol. 28, no. 1, pp. 181-186, 1998.
 S. A. Tengku and M. Norhayati, "Public health and clinical importance of amoebiasis in Malaysia: a review," Tropical Biomedicine, vol. 28, no. 2, pp. 194-222, 2011.
 M. Romeralo, R. Escalante, and S. L. Baldauf, "Evolution and diversity of dictyostelid social amoebae," Protist, vol. 163, no. 3, pp. 327-343, 2012.
 H. Scholze and W. Schulte, "On the specificity of a cysteine proteinase from Entamoeba histolytica," Biomedica Biochimica Acta, vol. 47, no. 2, pp. 115-123, 1988.
 A. L. Luaces and A. J. Barrett, "Affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of Entamoeba histolytica," Biochemical Journal, vol. 250, no. 3, pp. 903-909, 1988.
 R. Ocadiz, E. Orozco, E. Carrillo et al., "EhCP112 is an Entamoeba histolytica secreted cysteine protease that may be involved in the parasite-virulence," Cellular Microbiology, vol. 7, no. 2, pp. 221-232, 2005.
 A. Hellberg, N. Nowak, M. Leippe, E. Tannich, and I. Bruchhaus, "Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica," Protein Expression and Purification, vol. 24, no. 1, pp. 131-137, 2002.
 R. Thibeaux, A. Dufour, P. Roux, M. Bernier, A. C. Baglin, and P. Frileux, "Newly visualized fibrillar collagen scaffolds dictate Entamoeba histolytica invasion route in the human colon," Cellular Microbiology, vol. 14, no. 5, pp. 609-621, 2012.
 W. E. Keene, M. G. Petitt, S. Allen, and J. H. McKerrow, "The major neutral proteinase of Entamoeba histolytica," Journal of Experimental Medicine, vol. 163, no. 3, pp. 536-549, 1986.
 J. De Jesus Serrano, M. De La Garza, M. A. Moreno et al., "Entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant BG-3," Molecular Microbiology, vol. 11, no. 4, pp. 787-792, 1994.
 W. Schulte and H. Scholze, "Action of the major protease from Entamoeba histolytica on proteins of the extracellular matrix," Journal of Protozoology, vol. 36, no. 6, pp. 538-543, 1989.
 C. He, G. P. Nora, E. L. Schneider et al., "A novel Entamoeba histolytica cysteine proteinase, EhCP4, is key for invasive amebiasis and a therapeutic target," Journal of Biological Chemistry, vol. 285, no. 24, pp. 18516-18527, 2010.
 J. De Jesus Serrano, M. De La Garza, M. Reyes, G. Leon, R. Tovar, and M. De Lourdes Munoz, "Entamoeba histolytica: proteinase secretion induced by collagen type I is dependent on cytoskeleton integrity," Parasitology Research, vol. 82, no. 3, pp. 200-205, 1996.
 B. L. Kelsall and J. I. Ravdin, "Degradation of human IgA by Entamoeba histolytica," Journal of Infectious Diseases, vol. 168, no. 5, pp. 1319-1322, 1993.
 S. G. Melendez-Lopez, S. Herdman, K. Hirata et al., "Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model," Eukaryotic Cell, vol. 6, no. 7, pp. 1130-1136, 2007.
 S. L. Reed, W. E. Keene, J. H. McKerrow, and I. Gigli, "Cleavage of C3 by a neutral cysteine proteinase of Entamoeba histolytica," Journal of Immunology, vol. 143, no. 1, pp. 189-195, 1989.
 S. L. Reed, J. A. Ember, D. S. Herdman, R. G. DiScipio, T. E. Hugli, and I. Gigli, "The extracellular neutral cysteine proteinase of Entamoeba histolytica degrades anaphylatoxins C3a and C5a," Journal of Immunology, vol. 155, no. 1, pp. 266-274, 1995.
 X. Que, S. H. Kim, M. Sajid et al., "A surface amebic cysteine proteinase inactivates interleukin-18," Infection and Immunity, vol. 71, no. 3, pp. 1274-1280, 2003.
 E. Li, W. G. Yang, T. Zhang, and S. L. Stanley, "Interaction of laminin with Entamoeba histolytica cysteine proteinases and its effect on amebic pathogenesis," Infection and Immunity, vol. 63, no. 10, pp. 4150-4153, 1995.
 Z. Zhang, L. Wang, K. B. Seydel et al., "Entamoeba histolytica cysteine proteinases with interleukin-1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis," Molecular Microbiology, vol. 37, no. 3, pp. 542-548, 2000.
 J. McLaughlin and G. Faubert, "Partial purification and some properties of a neutral sulfhydryl and an acid proteinase from Entamoeba histolytica," Canadian Journal of Microbiology, vol. 23, no. 4, pp. 420-425, 1977.
 R. Perez-Montfort, P. Ostoa-Saloma, L. Velazquez-Medina, I. Montfort, and I. Becker, "Catalytic classes of proteinases of Entamoeba histolytica,"Molecular and Biochemical Parasitology, vol. 26, no. 1-2, pp. 87-97, 1987.
 W. B. Lushbaugh, A. F. Hofbauer, and A. A. Kairalla, "Relationship of cytotoxins of axenically cultivated Entamoeba histolytica to virulence," Gastroenterology, vol. 86, no. 6, pp. 1488-1495, 1984.
 W. B. Lushbaugh, A. F. Hofbauer, and F. E. Pittman, "Entamoeba histolytica: purification of cathepsin B," Experimental Parasitology, vol. 59, no. 3, pp. 328-336, 1985.
 J. De Jesus Serrano-Luna, E. Negrete, M. Reyes, and M. De La Garza, "Entamoeba histolytica HM1:IMSS: hemoglobin-degrading neutral cysteine proteases," Experimental Parasitology, vol. 89, no. 1, pp. 71-77, 1998.
 M. Reyes-Lopez, R. M. Bermudez-Cruz, E. E. Avila, and M. De La Garza, "Acetaldehyde/alcohol dehydrogenase-2 (EhADH2) and clathrin are involved in internalization of human transferrin by Entamoeba histolytica," Microbiology, vol. 157, no. 1, pp. 209-219, 2011.
 N. Leon-Sicairos, M. Reyes-Lopez, A. Canizalez-Roman et al., "Human hololactoferrin: endocytosis and use as an iron source by the parasite Entamoeba histolytica," Microbiology, vol. 151, no. 12, pp. 3859-3871, 2005.
 F. Lopez-Soto, A. Gonzalez-Robles, L. Salazar-Villatoro et al., "Entamoeba histolytica uses ferritin as an iron source and internalises this protein by means of clathrin-coated vesicles," International Journal for Parasitology, vol. 39, no. 4, pp. 417-426, 2009.
 D. Moncada, K. Keller, S. Ankri, D. Mirelman, and K. Chadee, "Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation," Gastroenterology, vol. 130, no. 3, pp. 721-730, 2006.
 R. Perez-Tamayo, I. Becker, I. Montfort, P. Ostoa-Saloma, and R. Perez-Montfort, "Role of leukocytes and amebic proteinases in experimental rat testicular necrosis produced by Entamoeba histolytica," Parasitology Research, vol. 77, no. 3, pp. 192-196, 1991.
 R. Lopez-Revilla and M. Baez-Camargo, "Immediate autoproteolysis and new proteinases in Entamoeba invadens and Entamoeba moshkovskii trophozoites," Archives of Medical Research, vol. 23, no. 2, pp. 95-97, 1992.
 V. Kissoon-Singh, L. Mortimer, and K. Chadee, "Entamoeba histolytica cathepsin-like enzymes: interactions with the host gut," Advances in Experimental Medicine and Biology, vol. 712, pp. 62-83, 2011.
 I. Bruchhaus, T. Jacobs, M. Leippe, and E. Tannich, "Entamoeba histolytica and Entamoeba dispar: differences in numbers and expression of cysteine proteinase genes," Molecular Microbiology, vol. 22, no. 2, pp. 255-263, 1996.
 A.Makioka, M. Kumagai, S. Kobayashi, and T. Takeuchi, "Entamoeba invadens: cysteine protease inhibitors block excystation and metacystic development," Experimental Parasitology, vol. 109, no. 1, pp. 27-32, 2005.
 A. Makioka, M. Kumagai, S. Kobayashi, and T. Takeuchi, "Involvement of serine proteases in the excystation and metacystic development of Entamoeba invadens," Parasitology Research, vol. 105, no. 4, pp. 977-987, 2009.
 M. Sharma, K. Hirata, S. Herdman, and S. Reed, "Entamoeba invadens: characterization of cysteine proteinases," Experimental Parasitology, vol. 84, no. 1, pp. 84-91, 1996.
 Y. Riahi and S. Ankri, "Involvement of serine proteinases during encystation of Entamoeba invadens," Archives of Medical Research, vol. 31, no. 4, pp. S187-S189, 2000.
 H. Scholze and W. Schulte, "Purification and partial characterization of the major cysteine protease from Entamoeba invadens," Biomedica Biochimica Acta, vol. 49, no. 6, pp. 455-463, 1990.
 F. R. De Souza Carvalho, L. C. Carrijo-Carvalho, A. M. Chudzinski-Tavassi, A. S. Foronda, and D. de Freitas, "Serine-like proteolytic enzymes correlated with differential pathogenicity in patients with acute Acanthamoeba keratitis," Clinical Microbiology and Infection, vol. 17, no. 4, pp. 603-609, 2011.
 B. K. Na, J. C. Kim, and C. Y. Song, "Characterization and pathogenetic role of proteinase fromAcanthamoeba castellanii," Microbial Pathogenesis, vol. 30, no. 1, pp. 39-48, 2001.
 M. Hurt, S. Neelam, J. Niederkorn, and H. Alizadeh, "Pathogenic Acanthamoeba spp. secrete a mannose-induced cytolytic protein that correlates with the ability to cause disease," Infection and Immunity, vol. 71, no. 11, pp. 6243-6255, 2003.
 M. Hurt, J. Niederkorn, and H. Alizadeh, "Effects of mannose on Acanthamoeba castellanii proliferation and cytolytic ability to corneal epithelial cells," Investigative Ophthalmology and Visual Science, vol. 44, no. 8, pp. 3424-3431, 2003.
 J. Sissons, S. Alsam, G. Goldsworthy, M. Lightfoot, E. L. Jarroll, and N. A. Khan, "Identification and properties of proteases from an Acanthamoeba isolate capable of producing granulomatous encephalitis," BMC Microbiology, vol. 6, article 42, 2006.
 H. K. Kim, Y. R. Ha, H. S. Yu, H. H. Kong, and D. I. Chung, "Purification and characterization of a 33 kDa serine protease From Acanthamoeba lugdunensis KA/E2 isolated from a Korean keratitis patient, "The Korean Journal of Parasitology, vol. 41, no. 4, pp. 189-196, 2003.
 J. D. J. Serrano-Luna, I. Cervantes-Sandoval, J. Calderon, F. Navarro-Garcia, V. Tsutsumi, and M. Shibayama, "Protease activities of Acanthamoeba polyphaga and Acanthamoeba castellanii," Canadian Journal of Microbiology, vol. 52, no. 1, pp. 16-23, 2006.
 K. Aldape, H. Huizinga, J. Bouvier, and J. McKerrow, "Naegleria fowleri: characterization of a secreted histolytic cysteine protease," Experimental Parasitology, vol. 78, no. 2, pp. 230-241, 1994.
 I. Cervantes-Sandoval, J. D. J. Serrano-Luna, E. Garcia-Latorre, V. Tsutsumi, and M. Shibayama, "Mucins in the host defence against Naegleria fowleri and mucinolytic activity as a possible means of evasion," Microbiology, vol. 154, no. 12, pp. 3895-3904, 2008.
 J. Serrano-Luna, I. Cervantes-Sandoval, V. Tsutsumi, and M. Shibayama, "A biochemical comparison of proteases from pathogenic Naegleria fowleri and non-pathogenic Naegleria gruberi," Journal of Eukaryotic Microbiology, vol. 54, no. 5, pp. 411-417, 2007.
 A. Matin, M. Stins, K. S. Kim, and N. A. Khan, "Balamuthia mandrillaris exhibits metalloprotease activities," FEMS Immunology and Medical Microbiology, vol. 47, no. 1, pp. 83-91, 2006.
 G. S. Visvesvara, H. Moura, and F. L. Schuster, "Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea," FEMS Immunology and Medical Microbiology, vol. 50, no. 1, pp. 1-26, 2007.
 A. Martinez-Palomo, "The pathogenesis of amoebiasis," Parasitology Today, vol. 3, no. 4, pp. 111-118, 1987.
 C. Ximenez, "Epidemiology of amebiasis inMexico: amolecular approach," Archives of Medical Research, vol. 37, no. 2, pp. 263-265, 2006.
 M. Espinosa-Cantellano and A. Martinez-Palomo, "Pathogenesis of intestinal amebiasis: from molecules to disease," Clinical Microbiology Reviews, vol. 13, no. 2, pp. 318-331, 2000.
 S. L. Stanley Jr., "Amoebiasis," Lancet, vol. 361, no. 9362, pp. 1025-1034, 2003.
 K. S. Ralston and W. A. Petri Jr., "Tissue destruction and invasion by Entamoeba histolytica," Trends in Parasitology, vol. 27, no. 6, pp. 254-263, 2011.
 K. Prathap and R. Gilman, "The histopathology of acute intestinal amebiasis. A rectal biopsy study," American Journal of Pathology, vol. 60, no. 2, pp. 229-246, 1970.
 R. Arroyo and E. Orozoco, "Localization and identification of an Entamoeba histolytica adhesin," Molecular and Biochemical Parasitology, vol. 23, no. 2, pp. 151-158, 1987.
 G. Garcia-Rivera, M. A. Rodriguez, R.Ocadiz et al., "Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112 kDa surface protein," Molecular Microbiology, vol. 33, no. 3, pp. 556-568, 1999.
 J. I. Ravdin, "Entamoeba histolytica: from adherence to enteropathy," Journal of Infectious Diseases, vol. 159, no. 3, pp. 420-429, 1989.
 W. A. Petri Jr. and J. I. Ravdin, "Cytopathogenicity of Entamoeba histolytica: the role of amebic adherence and contact-dependent cytolysis in pathogenesis," European Journal of Epidemiology, vol. 3, no. 2, pp. 123-136, 1987.
 J. L. Rosales-Encina, I. Meza, A. Lopex-De-Leon, P. Talamas-Rohana, and M. Rojkind, "Isolation of a 220-kilodalton protein with lectin properties from a virulent strain of Entamoeba histolytica," Journal of Infectious Diseases, vol. 156, no. 5, pp. 790-797, 1987.
 W. A. Petri Jr., R. Haque, and B. J. Mann, "The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica," Annual Review of Microbiology, vol. 56, pp. 39-64, 2002.
 J. J. McCoy, B. J. Mann, and W. A. Petri Jr., "Adherence and cytotoxicity of Entamoeba histolytica or how lectins let parasites stick around," Infection and Immunity, vol. 62, no. 8, pp. 3045-3050, 1994.
 M. Leippe and H. J. Muller-Eberhard, "The pore-forming peptide of Entamoeba histolytica, the protozoanparasite causing human amoebiasis," Toxicology, vol. 87, no. 1-3, pp. 5-18, 1994.
 J. I. Ravdin, F. Moreau, J. A. Sullivan, W. A. Petri Jr., and G. L. Mandell, "Relationship of free intracellular calcium to the cytolytic activity of Entamoeba histolytica," Infection and Immunity, vol. 56, no. 6, pp. 1505-1512, 1988.
 M. Leippe, S. Ebel, O. L. Schoenberger, R. D. Horstmann, and H. J. Muller-Eberhard, "Pore-forming peptide of pathogenic Entamoeba histolytica," Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 17, pp. 7659-7663, 1991.
 S. A. Long-Krug, K. J. Fischer, R. M. Hysmith, and J. I. Ravdin, "Phospholipase A enzymes of Entamoeba histolytica: description and subcellular localization," Journal of Infectious Diseases, vol. 152, no. 3, pp. 536-541, 1985.
 M. de la Torre, R. de la Hoz-Couturier, L. Landa, and B. Sepulveda, "Cultivos axenicos de Entamoeba histolytica," Archivos de Investigacion Medica, vol. 2, supplement 1, pp. 165-172, 1971.
 S. Ankri, T. Stolarsky, R. Bracha, F. Padilla-Vaca, and D. Mirelman, "Antisense inhibition of expression of cysteine proteinases affects Entamoeba histolytica-induced formation of liver abscess in hamsters," Infection and Immunity, vol. 67, no. 1, pp. 421-422, 1999.
 R. Bracha, Y. Nuchamowitz, M. Anbar, and D. Mirelman, "Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica," PLoS Pathogens, vol. 2, no. 5, article e48, 2006.
 I. Bruchhaus, B. J. Loftus, N. Hall, and E. Tannich, "The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation," Eukaryotic Cell, vol. 2, no. 3, pp. 501-509, 2003.
 J. H. McKerrow, E. Sun, P. J. Rosenthal, and J. Bouvier, "The proteases and pathogenicity of parasitic protozoa," Annual Review of Microbiology, vol. 47, pp. 821-853, 1993.
 X. Que, L. S. Brinen, P. Perkins et al., "Cysteine proteinases from distinct cellular compartments are recruited to phagocytic vesicles by Entamoeba histolytica," Molecular and Biochemical Parasitology, vol. 119, no. 1, pp. 23-32, 2002.
 A. E. Eakin, J. Bouvier, J. A. Sakanari, C. S. Craik, and J. H. McKerrow, "Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites," Molecular and Biochemical Parasitology, vol. 39, no. 1, pp. 1-8, 1990.
 S. Reed, J. Bouvier, A. S. Pollack et al., "Cloning of a virulence factor of Entamoeba histolytica. Pathogenic strains possess a unique cysteine proteinase gene," Journal of Clinical Investigation, vol. 91, no. 4, pp. 1532-1540, 1993.
 M. Tillack, L. Biller, H. Irmer et al., "The Entamoeba histolytica genome: primary structure and expression of proteolytic enzymes," BMC Genomics, vol. 8, article 170, 2007.
 C. A. Gilchrist, E. Houpt, N. Trapaidze et al., "Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome," Molecular and Biochemical Parasitology, vol. 147, no. 2, pp. 163-176, 2006.
 H. Scholze and E. Werries, "A weakly acidic protease has a powerful proteolytic activity in Entamoeba histolytica," Molecular and Biochemical Parasitology, vol. 11, pp. 293-300, 1984.
 H. Scholze, J. Otte, and E. Werries, "Cysteine proteinase of Entamoeba histolytica--II. Identification of the major split position in bovine insulin B-chain," Molecular and Biochemical Parasitology, vol. 18, no. 1, pp. 113-121, 1986.
 H. Scholze and E. Werries, "Cysteine proteinase of Entamoeba histolytica--I. Partial purification and action on different enzymes,"Molecular and Biochemical Parasitology, vol. 18, no. 1, pp. 103-112, 1986.
 E. Tannich, H. Scholze, R. Nickel, and R. D. Horstmann, "Homologous cysteine proteinases of pathogenic and nonpathogenic Entamoeba histolytica: differences in structure and expression," Journal of Biological Chemistry, vol. 266, no. 8, pp. 4798-4803, 1991.
 D. Mirelman, Y. Nuchamowitz, B. Bohm-Gloning, and B. Walderich, "Ahomologue of the cysteine proteinase gene (ACP1 or Eh-CPp3) of pathogenic Entamoeba histolytica is present in non-pathogenic E. dispar strains," Molecular and Biochemical Parasitology, vol. 78, no. 1-2, pp. 47-54, 1996.
 K. K. Hirata, X. Que, S. G. Melendez-Lopez et al., "A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release," Experimental Parasitology, vol. 115, no. 2, pp. 192-199, 2007.
 P. H. Davis, J. Schulze, and S. L. Stanley, "Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin," Molecular and Biochemical Parasitology, vol. 151, no. 1, pp. 118-128, 2007.
 T. Jacobs, I. Bruchhaus, T. Dandekar, E. Tannich, and M. Leippe, "Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica," Molecular Microbiology, vol. 27, no. 2, pp. 269-276, 1998.
 E. M. Duncan, T. L. Muratore-Schroeder, R. G. Cook et al., "Cathepsin L proteolytically processes histone H3 during mouse embryonic stem cell differentiation," Cell, vol. 135, no. 2, pp. 284-294, 2008.
 B. Goulet, A. Baruch, N. S. Moon et al., "A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor," Molecular Cell, vol. 14, no. 2, pp. 207-219, 2004.
 G. Maubach, M. C. C. Lim, and L. Zhuo, "Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells," Molecular Biology of the Cell, vol. 19, no. 10, pp. 4238-4248, 2008.
 S. L. Reed, W. E. Keene, and J. H. McKerrow, "Thiol proteinase expression and pathogenicity of Entamoeba histolytica," Journal of Clinical Microbiology, vol. 27, no. 12, pp. 2772-2777, 1989.
 U. Willhoeft, L. Hamann, and E. Tannich, "A DNA sequence corresponding to the gene encoding cysteine proteinase 5 in Entamoeba histolytica is present and positionally conserved but highly degenerated in Entamoeba dispar," Infection and Immunity, vol. 67, no. 11, pp. 5925-5929, 1999.
 S. Ankri, T. Stolarsky, and D. Mirelman, "Antisense inhibition of expression of cysteine proteinases does not affect Entamoeba histolytica cytopathic or haemolytic activity but inhibits phagocytosis," Molecular Microbiology, vol. 28, no. 4, pp. 777-785, 1998.
 M. A. R. Freitas, H. C. Fernandes, V. C. Calixto et al., "Entamoeba histolytica: cysteine proteinase activity and virulence. Focus on cysteine proteinase 5 expression levels," Experimental Parasitology, vol. 122, no. 4, pp. 306-309, 2009.
 L. I. Quintas-Granados, E. Orozco, L. G. Brieba, R. sArroyo, and J. Ortega-Lopez, "Purification, refolding and autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica," Protein Expression and Purification, vol. 63, no. 1, pp. 26-32, 2009.
 S. Carpeniseanu, K. Hirata, X. Que, E. Orozco, and S. L. Reed, "L6: a proteinase- and phagocytosis-deficient mutant of Entamoeba histolytica," Archives of Medical Research, vol. 31, no. 4, pp. S237-S238, 2000.
 X. Madriz, M. B. Martinez, M. A. Rodriguez et al., "Expression in fibroblasts and in live animals of Entamoeba histolytica polypeptides EhCP112 and EhADH112," Microbiology, vol. 150, no. 5, pp. 1251-1260, 2004.
 C. Martinez-Lopez, E. Orozco, T. Sanchez, R. M. Garcia-Perez, F. Hernandez-Hernandez, and M. A. Rodriguez, "The EhADH112 recombinant polypeptide inhibits cell destruction and liver abscess formation by Entamoeba histolytica trophozoites," Cellular Microbiology, vol. 6, no. 4, pp. 367-376, 2004.
 M. A. Rodriguez, F. Hernandez, L. Santos, A. Valdez, and E. Orozco, "Entamoeba histolytica: molecules involved in the target cell-parasite relationship," Molecular and Biochemical Parasitology, vol. 37, no. 1, pp. 87-100, 1989.
 A. Brittingham, C. J. Morrison, W. R. McMaster, B. S. McGwire, K. P. Chang, and D. M. Mosser, "Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis," Journal of Immunology, vol. 155, no. 6, pp. 3102-3111, 1995.
 D. M. Mosser and A. Brittingham, "Leishmania, macrophages and complement: a tale of subversion and exploitation," Parasitology, vol. 115, supplement, pp. S9-S23, 1997.
 W. A. Petri Jr., R. D. Smith, P. H. Schlesinger, C. F. Murphy, and J. I. Ravdin, "Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica," Journal of Clinical Investigation, vol. 80, no. 5, pp. 1238-1244, 1987.
 S. L. Stanley, K. Tian, J. P. Koester, and E. Li, "The serine-rich Entamoeba histolytica protein is a phosphorylated membrane protein containing O-linked terminal N-acetylglucosamine residues," Journal of Biological Chemistry, vol. 270, no. 8, pp. 4121-4126, 1995.
 J. E. Teixeira and C. D. Huston, "Participation of the serine-rich Entamoeba histolytica protein in amebic phagocytosis of apoptotic host cells," Infection and Immunity, vol. 76, no. 3, pp. 959-966, 2008.
 W. E. Keene, M. E. Hidalgo, E. Orozco, and J. H. McKerrow, "Entamoeba histolytica: correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase," Experimental Parasitology, vol. 71, no. 2, pp. 199-206, 1990.
 E. Bier, L. Y. Jan, and Y. N. Jan, "rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster," Genes and Development, vol. 4, no. 2, pp. 190-203, 1990.
 U. Mayer and C. Nusslein-Volhard, "A group of genes required for pattern formation in the ventral ectodermof the Drosophila embryo," Genes & Development, vol. 2, no. 11, pp. 1496-1511, 1988.
 J.M. Santos, A. Graindorge, and D. Soldati-Favre, "New insights into parasite rhomboid proteases," Molecular and Biochemical Parasitology, vol. 182, no. 1-2, pp. 27-36, 2012.
 S. Urban and M. Freeman, "Substrate specificity of Rhomboid intramembrane proteases is governed by helix-breaking residues in the substrate transmembrane domain," Molecular Cell, vol. 11, no. 6, pp. 1425-1434, 2003.
 L. A. Baxt, R. P. Baker, U. Singh, and S. Urban, "An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion," Genes and Development, vol. 22, no. 12, pp. 1636-1646, 2008.
 L. A. Baxt, E. Rastew, R. Bracha, D. Mirelman, and U. Singh, "Downregulation of an Entamoeba histolytica rhomboid protease reveals roles in regulating parasite adhesion and phagocytosis," Eukaryotic Cell, vol. 9, no. 8, pp. 1283-1293, 2010.
 K. Karakasis, D. Taylor, and K. Ko, "Uncovering a link between a plastid translocon component and rhomboid proteases using yeastmitochondria-based assays," Plant andCell Physiology, vol. 48, no. 4, pp. 655-661, 2007.
 P. C. Brooks, S. Stromblad, L. C. Sanders et al., "Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin [alpha]v[beta]3," Cell, vol. 85, no. 5, pp. 683-693, 1996.
 A. M. Lechner, I. Assfalg-Machleidt, S. Zahler et al., "RGD-dependent binding of procathepsin X to integrin [alpha] v[beta]3 mediates cell-adhesive properties," Journal of Biological Chemistry, vol. 281, no. 51, pp. 39588-39597, 2006.
 A. Mondino and F. Blasi, "uPA and uPAR in fibrinolysis, immunity and pathology," Trends in Immunology, vol. 25, no. 8, pp. 450-455, 2004.
 M. E. Papaconstantinou, C. J. Carrell, A. O. Pineda et al., "Thrombin functions through its RGD sequence in a noncanonical conformation," Journal of Biological Chemistry, vol. 280, no. 33, pp. 29393-29396, 2005.
 Y. Hou, L. Mortimer, and K. Chadee, "Entamoeba histolytica cysteine proteinase 5 binds integrin on colonic cells and stimulates NF[keppa]B-mediated pro-inflammatory responses," Journal of Biological Chemistry, vol. 285, no. 46, pp. 35497-35504, 2010.
 A. F. Horwitz, "Integrins and health," Scientific American, vol. 276, no. 5, pp. 68-75, 1997.
 L. Mortimer and K. Chadee, "The immunopathogenesis of Entamoeba histolytica," Experimental Parasitology, vol. 126, no. 3, pp. 366-380, 2010.
 A. Belley, K. Keller, M. Goettke, and K. Chadee, "Intestinal mucins in colonization and host defense against pathogens," American Journal of Tropical Medicine andHygiene, vol. 60, no. 4, pp. 10-15, 1999.
 N. Asker, M. A. B. Axelsson, S. O. Olofsson, and G. C. Hansson, "Dimerization of the human MUC2 mucin in the endoplasmic reticulum is followed by a N-glycosylation-dependent transfer of the mono- and dimers to the Golgi apparatus," Journal of Biological Chemistry, vol. 273, no. 30, pp. 18857-18863, 1998.
 A. Herrmann, J. R. Davies, G. Lindell et al., "Studies on the 'insoluble' glycoprotein complex from human colon: identification of reduction-insensitive MUC2 oligomers and C-terminal cleavage," Journal of Biological Chemistry, vol. 274, no. 22, pp. 15828-15836, 1999.
 D. Bansal, P. Ave, S. Kerneis et al., "An ex vivo human intestinal model to study Entamoeba histolytica pathogenesis," PLoS Neglected Tropical Diseases, vol. 3, no. 11, article e551, 2009.
 D. Moncada, K. Keller, and K. Chadee, "Entamoeba histolytica cysteine proteinases disrupt the polymeric structure of colonic mucin and alter its protective function," Infection and Immunity, vol. 71, no. 2, pp. 838-844, 2003.
 M. E. Lidell, D. M. Moncada, K. Chadee, and G. C. Hansson, "Entamoeba histolytica cysteine protease cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel," Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 24, pp. 9298-9303, 2006.
 D. Moncada, K. Keller, and K. Chadee, "Entamoeba histolytica-secreted products degrade colonic mucin oligosaccharides," Infection and Immunity, vol. 73, no. 6, pp. 3790-3793, 2005.
 A. Debnath, J. S. Tashker, M. Sajid, and J. H. McKerrow, "Transcriptional and secretory responses of Entamoeba histolytica to mucins, epithelial cells and bacteria," International Journal for Parasitology, vol. 37, no. 8-9, pp. 897-906, 2007.
 A. Leroy, T. Lauwaet, G. De Bruyne, M. Cornelissen, and M. Mareel, "Entamoeba histolytica disturbs the tight junction complex in human enteric T84 cell layers," FASEB Journal, vol. 14, no. 9, pp. 1139-1146, 2000.
 A. Leroy, T. Lauwaet, M. J. Oliveira et al., "Disturbance of tight junctions by Entamoeba histolytica: resistant vertebrate cell types and incompetent trophozoites," Archives of Medical Research, vol. 31, no. 4, pp. S218-S220, 2000.
 T. Lauwaet, M. J. Oliveira, B. Callewaert et al., "Proteolysis of enteric cell villin by Entamoeba histolytica cysteine proteinases," Journal of Biological Chemistry, vol. 278, no. 25, pp. 22650-22656, 2003.
 T. Lauwaet, M. J. Oliveira, B. Callewaert, G. De Bruyne, M. Mareel, and A. Leroy, "Proteinase inhibitors TPCK and TLCK prevent Entamoeba histolytica induced disturbance of tight junctions and microvilli in enteric cell layers in vitro," International Journal for Parasitology, vol. 34, no. 7, pp. 785-794, 2004.
 C. Gitler, E. Calef, and I. Rosenberg, "Cytopathogenicity of Entamoeba histolytica," Philosophical Transactions of the Royal Society B, vol. 307, no. 1131, pp. 73-85, 1984.
 X. Que and S. L. Reed, "Cysteine proteinases and the pathogenesis of amebiasis," Clinical Microbiology Reviews, vol. 13, no. 2, pp. 196-206, 2000.
 S. Spinella, E. Levavasseur, F. Petek, and M. C. Rigothier, "Purification and biochemical characterization of a novel cysteine protease of Entamoeba histolytica," European Journal of Biochemistry, vol. 266, no. 1, pp. 170-180, 1999.
 S. L. Reed, J. G. Curd, and I. Gigli, "Activation of complement by pathogenic and nonpathogenic Entamoeba histolytica," Journal of Immunology, vol. 136, no. 6, pp. 2265-2270, 1986.
 M. D. L. Munoz, J. Calderon, and M. Rojkind, "The collagenase of Entamoeba histolytica," Journal of Experimental Medicine, vol. 155, no. 1, pp. 42-51, 1982.
 M. De Lourdes Munoz, E. Lamoyi, G. Leon et al., "Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity," Journal of Clinical Microbiology, vol. 28, no. 11, pp. 2418-2424, 1990.
 A. Debnath, M. A. Akbar, A. Mazumder, S. Kumar, and P. Das, "Entamoeba histolytica: characterization of human collagen type I and Ca2+ activated differentially expressed genes," Experimental Parasitology, vol. 110, no. 3, pp. 214-219, 2005.
 H. Gadasi and E. Kessler, "Correlation of virulence and collagenolytic activity in Entamoeba histolytica," Infection and Immunity, vol. 39, no. 2, pp. 528-531, 1983.
 M. A. Magos, M. De La Torre, and M. I. Munoz, "Collagenase activity in clinical isolates of Entamoeba histolytica maintained in xenic cultures," Archives of Medical Research, vol. 23, no. 2, pp. 115-118, 1992.
 M. De Lourdes Munoz, M. Rojkind, and J. Calderon, "Entamoeba histolytica: collagenolytic activity and virulence," Journal of Protozoology, vol. 31, no. 3, pp. 468-470, 1984.
 V. Tsutsumi, A. Ramirez-Rosales, H. Lanz-Mendoza et al., "Entamoeba histolytica: erythrophagocytosis, collagenolysis, and liver abscess production as virulencemarkers," Transactions of the Royal Society of TropicalMedicine andHygiene, vol. 86, no. 2, pp. 170-172, 1992.
 B. Chavez-Munguia, G. Castanon, V. Hernandez-Ramirez, M. Gonzalez-Lazaro, P. Talamas-Rohana, and A. Martinez-Palomo, "Entamoeba histolytica electrondense granules secretion in vitro and in vivo: ultrastructural study," Microscopy Research and Technique, vol. 75, no. 2, pp. 189-196, 2012.
 P. Talamas-Rohana and I. Meza, "Interaction between pathogenic amebas and fibronectin: substrate degradation and changes in cytoskeleton organization," Journal of Cell Biology, vol. 106, no. 5, pp. 1787-1794, 1988.
 I. Meza, "Extracellular matrix-induced signaling in Entamoeba histolytica: its role in invasiveness," Parasitology Today, vol. 16, no. 1, pp. 23-28, 2000.
 J. Vazquez-Prado and I. Meza, "Fibronectin 'receptor' in Entamoeba histolytica: purification and association with the cytoskeleton," Archives of Medical Research, vol. 23, no. 2, pp. 125-128, 1992.
 E. Franco, J. Vazquez-Prado, and I. Meza, "Fibronectin-derived fragments as inducers of adhesion and chemotaxis of Entamoeba histolytica trophozoites," Journal of Infectious Diseases, vol. 176, no. 6, pp. 1597-1602, 1997.
 S. L. Stanley Jr., T. Zhang, D. Rubin, and E. Li, "Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice," Infection and Immunity, vol. 63, no. 4, pp. 1587-1590, 1995.
 M. Tillack, N. Nowak, H. Lotter et al., "Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica,"Molecular and Biochemical Parasitology, vol. 149, no. 1, pp. 58-64, 2006.
 A. Hellberg, R. Nickel, H. Lotter, E. Tannich, and I. Bruchhaus, "Overexpression of cysteine proteinase 2 in Entamoeba histolytica or Entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils," CellularMicrobiology, vol. 3, no. 1, pp. 13-20, 2001.
 H. Irmer, M. Tillack, L. Biller et al., "Major cysteine peptidases of Entamoeba histolytica are required for aggregation and digestion of erythrocytes but are dispensable for phagocytosis and cytopathogenicity," Molecular Microbiology, vol. 72, no. 3, pp. 658-667, 2009.
 K.McGowan, C. F. Deneke, G. M. Thorne, and S. L. Gorbach, "Entamoeba histolytica cytotoxin: purification, characterization, strain virulence, and protease activity," Journal of Infectious Diseases, vol. 146, no. 5, pp. 616-625, 1982.
 D. M. Faust, J. M. Markiewicz, A. Danckaert, G. Soubigou, and N. Guillen, "Human liver sinusoidal endothelial cells respond to interaction with Entamoeba histolytica by changes in morphology, integrin signalling and cell death," Cellular Microbiology, vol. 13, no. 7, pp. 1091-1106, 2011.
 S. Kumar, R. Banerjee, N. Nandi, A. H. Sardar, and P. Das, "Anoikis potential of Entameba histolytica secretory cysteine proteases: evidence of contact independent host cell death," Microbial Pathogenesis, vol. 52, no. 1, pp. 69-76, 2012.
 J. M. Woof and M. A. Ken, "The function of immunoglobulin A in immunity," Journal of Pathology, vol. 208, no. 2, pp. 270-282, 2006.
 R. M. Garcia-Nieto, R. Rico-Mata, S. Arias-Negrete, and E. E. Avila, "Degradation of human secretory IgA1 and IgA2 by Entamoeba histolytica surface-associated proteolytic activity," Parasitology International, vol. 57, no. 4, pp. 417-423, 2008.
 J. C. Carrero, C. Cervantes-Rebolledo, H. Aguilar-Diaz, M. Y. Diaz-Gallardo, J. P. Laclette, and J. Morales-Montor, "The role of the secretory immune response in the infection by Entamoeba histolytica," Parasite Immunology, vol. 29, no. 7, pp. 331-338, 2007.
 B. L. Kelsall, T. G. F. H. Jackson, V. Gathiram et al., "Secretory immunoglobulin A antibodies to the galactose-inhibitable adherence protein in the saliva of patients with amebic liver disease," American Journal of Tropical Medicine and Hygiene, vol. 51, no. 4, pp. 454-459, 1994.
 J. C. Carrero, M. Y. Diaz, M. Viveros, B. Espinoza, E. Acosta, and L. Ortiz-Ortiz, "Human secretory immunoglobulin A anti-Entamoeba histolytica antibodies inhibit adherence of amebae toMDCK cells," Infection and Immunity, vol. 62, no. 2, pp. 764-767, 1994.
 G. G. Guerrero-Manriquez, F. Sanchez-Ibarra, and E. E. Avila, "Inhibition of Entamoeba histolytica proteolytic activity by human salivary IgA antibodies," Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 106, no. 11, pp. 1088-1094, 1998.
 N. Leon-Sicairos, F. Lopez-Soto, M. Reyes-Lopez, D. Godinez-Vargas, C. Ordaz-Pichardo, and M. De LaGarza, "Amoebicidal activity of milk, apo-lactoferrin, slgA and lysozyme," Clinical Medicine and Research, vol. 4, no. 2, pp. 106-113, 2006.
 R. Haque, D. Mondal, P. Duggal et al., "Entamoeba histolytica infection in children and protection from subsequent amebiasis," Infection and Immunity, vol. 74, no. 2, pp. 904-909, 2006.
 M. D. Abd Alla, R. Wolf, G. L. White, S. D. Kosanke, D. Cary, and J. J. Verweij, "Efficacy of a Gal-lectin subunit vaccine against experimental Entamoeba histolytica infection and colitis in baboons (Papio sp.)," Vaccine, vol. 30, no. 20, pp. 3068-3075, 2012.
 T. F. H. G. Jackson and V. Gathiram, "Seroepidemiological study of antibody responses to the zymodemes of Entamoeba histolytica," Lancet, vol. 1, no. 8431, pp. 716-719, 1985.
 J. C. Carrero, A. Contreras-Rojas, B. Sanchez-Hernandez et al., "Protection against murine intestinal amoebiasis induced by oral immunization with the 29kDa antigen of Entamoeba histolytica and cholera toxin," Experimental Parasitology, vol. 126, no. 3, pp. 359-365, 2010.
 D. M. Meneses-Ruiz, J. P. Laclette, H. Aguilar-Diaz, J. Hernandez-Ruiz, A. Luz-Madrigal, and A. Sampieri, "Mucosal delivery of ACNPV baculovirus driving expression of the Gallectin LC3 fragment confers protection against amoebic liver abscess in hamster," International Journal of Biological Sciences, vol. 7, no. 9, pp. 1345-1356, 2011.
 V. Q. Tran, D. S. Herdman, B. E. Torian, and S. L. Reed, "The neutral cysteine proteinase of Entamoeba histolytica degrades IgG and prevents its binding," Journal of Infectious Diseases, vol. 177, no. 2, pp. 508-511, 1998.
 D. Ricklin, G. Hajishengallis, K. Yang, and J. D. Lambris, "Complement: a key system for immune surveillance and homeostasis," Nature Immunology, vol. 11, no. 9, pp. 785-797, 2010.
 S. L. Reed, P. G. Sargeaunt, and A. I. Braude, "Resistance to lysis by human serum of pathogenic Entamoeba histolytica," Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 77, no. 2, pp. 248-253, 1983.
 R. Capin, N. R. Capin, M. Carmona, and L. Ortiz-Ortiz, "Effect of complement depletion on the induction of amebic liver abscess in the hamster," Archivos de Investigacion Medica, vol. 11, no. 1, pp. 173-180, 1980.
 S. L. Reed and I. Gigli, "Lysis of complement-sensitive Entamoeba histolytica by activated terminal complement components. Initiation of complement activation by an extracellular neutral cysteine proteinase," Journal of Clinical Investigation, vol. 86, no. 6, pp. 1815-1822, 1990.
 A. Olivos-Garcia, E. Saavedra, E. Ramos-Martinez, M. Nequiz, and R. Perez-Tamayo, "Molecular nature of virulence in Entamoeba histolytica," Infection, Genetics and Evolution, vol. 9, no. 6, pp. 1033-1037, 2009.
 Q. Peng, K. Li, S. H. Sacks, and W. Zhou, "The role of anaphylatoxins C3a and C5a in regulating innate and adaptive immune responses," Inflammation and Allergy, vol. 8, no. 3, pp. 236-246, 2009.
 K. B. Seydel, E. Li, P. E. Swanson, and S. L. Stanley, "Human intestinal epithelial cells produce proinflammatory cytokines in response to infection in a SCID mouse-human intestinal xenograft model of amebiasis," Infection and Immunity, vol. 65, no. 5, pp. 1631-1639, 1997.
 R. Bracha, Y. Nuchamowitz, M. Leippe, and D. Mirelman, "Antisense inhibition of amoebapore expression in Entamoeba histolytica causes a decrease in amoebic virulence," Molecular Microbiology, vol. 34, no. 3, pp. 463-472, 1999.
 M. Leippe, "Amoebapores," Parasitology Today, vol. 13, no. 5, pp. 178-183, 1997.
 C. A. Dinarello, "Interleukin-18," Methods, vol. 19, no. 1, pp. 121-132, 1999.
 R. Seguin, B. J. Mann, K. Keller, and K. Chadee, "The tumor necrosis factor alpha-stimulating region of galactose-inhibitable lectin of Entamoeba histolytica activates gamma interferon- primed macrophages for amebicidal activity mediated by nitric oxide," Infection and Immunity, vol. 65, no. 7, pp. 2522-2527, 1997.
 R. L. Jurado, "Iron, infections, and anemia of inflammation," Clinical Infectious Diseases, vol. 25, no. 4, pp. 888-895, 1997.
 B. R. Otto, A. M. J. J. Verweij-Van Vught, and D. M. MacLaren, "Transferrins and heme-compounds as iron sources for pathogenic bacteria," Critical Reviews in Microbiology, vol. 18, no. 3, pp. 217-233, 1992.
 E. D. Weinberg, "Infection and iron metabolism," American Journal of Clinical Nutrition, vol. 30, no. 9, pp. 1485-1490, 1977.
 E. D. Weinberg, "Iron and infection," Microbiological Reviews, vol. 42, no. 1, pp. 45-66, 1978.
 E. D. Weinberg and J. Miklossy, "Iron withholding: a defense against disease," Journal of Alzheimer's Disease, vol. 13, no. 4, pp. 451-463, 2008.
 E. D. Weinberg, "Iron and susceptibility to infectious disease," Science, vol. 184, no. 4140, pp. 952-956, 1974.
 F. Lopez-Soto, N. Leon-Sicairos, M. Reyes-Lopez et al., "Use and endocytosis of iron-containing proteins by Entamoeba histolytica trophozoites," Infection, Genetics and Evolution, vol. 9, no. 6, pp. 1038-1050, 2009.
 J. Tachezy and M. Wilson, "More on iron acquisition by parasitic protozoa," Parasitology Today, vol. 15, no. 5, p. 207, 1999.
 S. Vanacova, D. Rasoloson, J. Razga, I. Hrdy, J. Kulda, and J. Tachezy, "Iron-induced changes in pyruvate metabolism of Tritrichomonas foetus and involvement of iron in expression of hydrogenosomal proteins," Microbiology, vol. 147, no. 1, pp. 53-62, 2001.
 R. A. Finkelstein, C. V. Sciortino, and M. A. McIntosh, "Role of iron in microbe-host interactions," Reviews of Infectious Diseases, vol. 5, supplement, pp. S759-S777, 1983.
 E. D. Weinberg, "The role of iron in protozoan and fungal infectious diseases," Journal of Eukaryotic Microbiology, vol. 46, no. 3, pp. 231-238, 1999.
 A. Jacobs and M. Worwood, Iron in Biochemistry and Medicine, Academic Press, London, UK, 1980.
 S. Said-Fernandez and R. Lopez-Revilla, "Subcellular distribution and stability of the major hemolytic activity of Entamoeba histolytica trophozoites," Zeitschrift fur Parasitenkunde, vol. 67, no. 3, pp. 249-254, 1982.
 V. Tsutsumi, A. Martinez-Palomo, and K. Tanikawa, "Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites," Archives of Medical Research, vol. 23, no. 2, pp. 173-175, 1992.
 J. Vargas-Villarreal, H. Martinez-Rodriguez, J. Castro-Garza, B. D. Mata-Cardenas, M. T. Gonzalez-Garza, and S. Said-Fernandez, "Identification of Entamoeba histolytica intracellular phospholipase A and lysophospholipase L1 activities," Parasitology Research, vol. 81, no. 4, pp. 320-323, 1995.
 A. Chevez, I. Iturbe-Alessio, M. Segura, and D. Corona, "Phagocytosis of human erythrocytes by Entamoeba histolytica," Archivos de Investigacion Medica, vol. 2, pp. 2-286, 1972.
 J. Mora-Galindo and F. Anaya-Velazquez, "Intracellular digestion of human erythrocytes by Entamoeba histolytica: a kinetic study in vitro," Archives of Medical Research, vol. 24, no. 4, pp. 347-351, 1993.
 J. Mora-Galindo, M. Gutierrez-Lozano, and F. Anaya-Velazquez, "Entamoeba histolytica: kinetics of hemolytic activity, erythrophagocytosis and digestion of erythrocytes," Archives of Medical Research, vol. 28, pp. 200-201, 1997.
 R. Jarumilinta and B. G. Maegraith, "The patterns of some proteolytic enzymes of Entamoeba histolytica and Acanthamoeba sp--I. The action of E. histolytica and Acanthamoeba sp. on protein substrates," Annals of Tropical Medicine and Parasitology, vol. 55, pp. 505-517, 1961.
 I. Becker, R. Perez-Montfort, A. Perez-Torres, A. Rondan-Zarate, I. Montfort, and R. Perez-Tamayo, "Entamoeba histolytica: localization of a 30-kDa cysteine proteinase using a monoclonal antibody," Experimental Parasitology, vol. 82, no. 2, pp. 171-181, 1996.
 J. Mora-Galindo, F. Anaya-Velazquez, S. Ramirez-Romo, and A. Gonzalez-Robles, "Entamoeba histolytica: correlation of assessment methods to measure erythrocyte digestion, and effect of cysteine proteinases inhibitors in HM-1:IMSS and HK-9:NIH strains," Experimental Parasitology, vol. 108, no. 3-4, pp. 89-100, 2004.
 A. Bezkorovainy, "Biochemistry of nonheme iron in man--I. Iron proteins and cellular iron metabolism," Clinical Physiology and Biochemistry, vol. 7, no. 1, pp. 1-17, 1989.
 J. A. Fernandez-Pol and D. J. Klos, "Isolation and characterization of Normal Rat Kidney cell membrane proteins with affinity for transferrin," Biochemistry, vol. 19, no. 17, pp. 3904-3912, 1980.
 M. Reyes-Lopez, J. D. J. Serrano-Luna, E. Negrete-Abascal, N. Leon-Sicairos, A. L. Guerrero-Barrera, and M. De la Garza, "Entamoeba histolytica: transferrin binding proteins," Experimental Parasitology, vol. 99, no. 3, pp. 132-140, 2001.
 J. Langhorst and J. Boone, "Fecal lactoferrin as a noninvasive biomarker in inflammatory bowel diseases," Drugs Today, vol. 48, no. 2, pp. 149-161, 2012.
 R. D. Brines and J. H. Brock, "The effect of trypsin and chymotrypsin on the in vitro antimicrobial and iron-binding properties of lactoferrin in human milk and bovine colostrum. Unusual resistance of human apolactoferrin to proteolytic digestion," Biochimica et Biophysica Acta, vol. 759, no. 3, pp. 229-235, 1983.
 P. Aisen and A. Leibman, "Lactoferrin and transferrin: a comparative study," Biochimica et Biophysica Acta, vol. 257, no. 2, pp. 314-323, 1972.
 E. N. Baker and H. M. Baker, "Molecular structure, binding properties and dynamics of lactoferrin," Cellular and Molecular Life Sciences, vol. 62, no. 22, pp. 2531-2539, 2005.
 J. A. Talbot, K. Nielsen, and L. B. Corbeil, "Cleavage of proteins of reproductive secretions by extracellular proteinases of Tritrichomonas foetus," Canadian Journal of Microbiology, vol. 37, no. 5, pp. 384-390, 1991.
 P. Arosio and S. Levi, "Ferritin, iron homeostasis, and oxidative damage," Free Radical Biology and Medicine, vol. 33, no. 4, pp. 457-463, 2002.
 P. M. Harrison and P. Arosio, "The ferritins: molecular properties, iron storage function and cellular regulation," Biochimica et Biophysica Acta, vol. 1275, no. 3, pp. 161-203, 1996.
 A. M. Koorts and M. Viljoen, "Ferritin and ferritin isoforms I: structure-function relationships, synthesis, degradation and secretion," Archives of Physiology and Biochemistry, vol. 113, no. 1, pp. 30-54, 2007.
 X. Liu and E. C. Theil, "Ferritins: dynamic management of biological iron and oxygen chemistry," Accounts of Chemical Research, vol. 38, no. 3, pp. 167-175, 2005.
 R. Lopez-Revilla and S. Said-Fernandez, "Cytopathogenicity of Entamoeba histolytica: hemolytic activity of trophozoite homogenates," American Journal of Tropical Medicine and Hygiene, vol. 29, no. 2, pp. 209-212, 1980.
 C. Shimokawa, M. Kabir, M. Taniuchi, D. Mondal, S. Kobayashi, and I. K. M. Ali, "Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice," Journal of Infectious Diseases, vol. 206, no. 5, pp. 744-751, 2012.
 C. G. Clark and L. S. Diamond, "The Laredo strain and other Entamoeba histolytica-like amoebae are Entamoeba moshkovskii," Molecular and Biochemical Parasitology, vol. 46, no. 1, pp. 11-18, 1991.
 R. D. Heredia, J. A. Fonseca, and M. C. Lopez, "Entamoeba moshkovskii perspectives of a new agent to be considered in the diagnosis of amebiasis," Acta Tropica, vol. 123, no. 3, pp. 139-145, 2012.
 M. Goldman, "Entamoeba histolytica-like amoebae ocurring in man," Bulletin of the World Health Organization, vol. 40, pp. 355-364, 1969.
 I. K. M. Ali, C. G. Clark, and W. A. Petri Jr., "Molecular epidemiology of amebiasis," Infection, Genetics and Evolution, vol. 8, no. 5, pp. 698-707, 2008.
 Z. Hamzah, S. Petmitr, M. Mungthin, S. Leelayoova, and P. Chavalitshewinkoon-Petmitr, "Development of multiplex real-time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens," American Journal of Tropical Medicine and Hygiene, vol. 83, no. 4, pp. 909-913, 2010.
 WHO, ""Amoebiasis," World Health Organization/Pan America Health Organization Expert Consultation on Amoebiasis," WHOWeekly Epidemiological Record, vol. 72, pp. 97-100, 1997.
 S. S. Dolabella, J. Serrano-Luna, F. Navarro-Garcia et al. et al., "Amoebic liver abscess production by Entamoeba dispar," Annals of Hepatology, vol. 11, no. 1, pp. 107-117, 2012.
 W. M. Spice and J. P. Ackers, "The effect of axenic versus xenic culture conditions on the total and secreted proteolytic activity of Entamoeba histolytica strains," Archives of Medical Research, vol. 23, no. 2, pp. 91-93, 1992.
 B. Loftus, I. Anderson, R. Davies et al., "The genome of the protist parasite Entamoeba histolytica," Nature, vol. 433, no. 7028, pp. 865-868, 2005.
 I. Bruchhaus and E. Tannich, "A gene highly homologous to ACP1 encoding cysteine proteinase 3 in Entamoeba histolytica is present and expressed in E. dispar," Parasitology Research, vol. 82, no. 2, pp. 189-192, 1996.
 E. Tannich, "Recent advances in DNA-mediated gene transfer of Entamoeba histolytica," Parasitology Today, vol. 12, no. 5, pp. 198-200, 1996.
 B. N. Mitra, T. Yasuda, S. Kobayashi, Y. Saito-Nakano, and T. Nozaki, "Differences in morphology of phagosomes and kinetics of acidification and degradation in phagosomes between the pathogenic Entamoeba histolytica and the non-pathogenic Entamoeba dispar," Cell Motility and the Cytoskeleton, vol. 62, no. 2, pp. 84-99, 2005.
 J. E. Teixeira, A. Sateriale, K. E. Bessoff, and C. D. Huston, "Control of Entamoeba histolytica adherence involves metallosurface protease 1, an M8 family surface metalloprotease with homology to leishmanolysin," Infection and Immunity, vol. 80, no. 6, pp. 2165-2176, 2012.
 F. Ebert, A. Bachmann, K. Nakada-Tsukui et al., "An Entamoeba cysteine peptidase specifically expressed during encystation," Parasitology International, vol. 57, no. 4, pp. 521-524, 2008. Journal of Tropical Medicine 31
 G. Jeelani, D. Sato, A. Husain, A. Escueta-de Cadiz, M. Sugimoto, and T. Soga, "Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation," PLoS ONE, vol. 7, no. 5, Article ID e37740, 2012.
 E. E. Avila, M. Sanchez-Garza, and J. Calderon, "Entamoeba histolytica and E. invadens: sulfhydryl-dependent proteolytic activity1," Journal of Eukaryotic Microbiology, vol. 32, no. 1, pp. 163-166, 1985.
 S. K. Ghosh, J. Field, M. Frisardi et al., "Chitinase secretion by encysting Entamoeba invadens and transfected Entamoeba histolytica trophozoites: localization of secretory vesicles, endoplasmic reticulum, and Golgi apparatus," Infection and Immunity, vol. 67, no. 6, pp. 3073-3081, 1999.
 K. L. Van Dellen, A. Chatterjee, D. M. Ratner et al., "Unique posttranslational modifications of chitin-binding lectins of Entamoeba invadens cyst walls," Eukaryotic Cell, vol. 5, no. 5, pp. 836-848, 2006.
 J. Gonzalez, G. Bai, U. Frevert, E. J. Corey, and D. Eichinger, "Proteasome-dependent cyst formation and stage-specific ubiquitin mRNA accumulation in Entamoeba invadens," European Journal of Biochemistry, vol. 264, no. 3, pp. 897-904, 1999.
 H. Trabelsi, F. Dendana, A. Sellami, H. Sellami, F. Cheikhrouhou, and S. Neji, "Pathogenic fre-living amoeba: epidemiology and clinical review," Pathologie Biologie, vol. 60, no. 6, pp. 399-405, 2012.
 M. Omana-Molina, F. Navarro-Garcia, A. Gonzalez-Robles, J. d. Serrano-Luna, R. Campos-Rodriguez, and A. Martinez-Palomo, "Induction of morphological and electrophysiological changes in hamster cornea after in vitro interaction with trophozoites of Acanthamoeba spp," Infection and Immunity, vol. 72, no. 6, pp. 3245-3251, 2004.
 M. Garate, Z. Cao, E. Bateman, and N. Panjwani, "Cloning and characterization of a novel mannose-binding protein of Acanthamoeba," Journal of Biological Chemistry, vol. 279, no. 28, pp. 29849-29856, 2004.
 Y. C. Hong, W. M. Lee, H. H. Kong, H. J. Jeong, and D. I. Chung, "Molecular cloning and characterization of a cDNA encoding a laminin-binding protein (AhLBP) from Acanthamoeba healyi," Experimental Parasitology, vol. 106, no. 3-4, pp. 95-102, 2004.
 B. Da Rocha-Azevedo, M. Jamerson, G. A. Cabral, F. C. Silva-Filho, and F.Marciano-Cabral, "Acanthamoeba interaction with extracellular matrix glycoproteins: biological and biochemical characterization and role in cytotoxicity and invasiveness," Journal of Eukaryotic Microbiology, vol. 56, no. 3, pp. 270-278, 2009.
 V. R. Gordon, E. K. Asem, M. H. Vodkin, and G. L. McLaughlin, "Acanthamoeba binds to extracellular matrix proteins in vitro," Investigative Ophthalmology and Visual Science, vol. 34, no. 3, pp. 658-662, 1993.
 L. Wang, E. K. Asem, and G. L. McLaughlin, "Calcium enhances Acanthamoeba polyphaga binding to extracellular matrix proteins," Investigative Ophthalmology and Visual Science, vol. 35, no. 5, pp. 2421-2426, 1994.
 J. Sissons, S. K. Kwang, M. Stins, S. Jayasekera, S. Alsam, and N. A. Khan, "Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism," Infection and Immunity, vol. 73, no. 5, pp. 2704-2708, 2005.
 H. Alizadeh, S. Neelam, M. Hurt, and J. Y. Niederkorn, "Role of contact lens wear, bacterial flora, and mannose-induced pathogenic protease in the pathogenesis of amoebic keratitis," Infection and Immunity, vol. 73, no. 2, pp. 1061-1068, 2005.
 A. Ferrante and E. J. Bates, "Elastase in the pathogenic free-living amoebae Naegleria and Acanthamoeba spp," Infection and Immunity, vol. 56, no. 12, pp. 3320-3321, 1988.
 P. N.Mortazavi, E. Keisary, L. N. Loh, S. Y. Jung, and N. A. Khan, "Possible roles of phospholipaseA2 in the biological activities of Acanthamoeba castellanii (T4 Genotype)," Protist, vol. 162, no. 1, pp. 168-176, 2011.
 S. C. Alfieri, C. E. B. Correia, S. A. Motegi, and E. M. F. Pral, "Proteinase activities in total extracts and in medium conditioned by Acanthamoeba polyphaga trophozoites," Journal of Parasitology, vol. 86, no. 2, pp. 220-227, 2000.
 E. Hadas and T. Mazur, "Proteolytic enzymes of pathogenic and non-pathogenic strains of Acanthamoeba spp," Tropical Medicine and Parasitology, vol. 44, no. 3, pp. 197-200, 1993.
 M. M. Mitra, H. Alizadeh, R. D. Gerard, and J. Y. Niederkorn, "Characterization of a plasminogen activator produced by Acanthamoeba castellanii," Molecular and Biochemical Parasitology, vol. 73, no. 1-2, pp. 157-164, 1995.
 K. Mitro, A. Bhagavathiammai, O. M. Zhou et al., "Partial characterization of the proteolytic secretions of Acanthamoeba polyphaga," Experimental Parasitology, vol. 78, no. 4, pp. 377-385, 1994.
 N. A. Khan, E. L. Jarroll, N. Panjwani, Z. Cao, and T. A. Paget, "Proteases as markers for differentiation of pathogenic and nonpathogenic species of Acanthamoeba," Journal of Clinical Microbiology, vol. 38, no. 8, pp. 2858-2861, 2000.
 Y. He, J. Y. Niederkorn, J. P. McCulley et al., "In vivo and in vitro collagenolytic activity of Acanthamoeba castellanii," Investigative Ophthalmology and Visual Science, vol. 31, no. 11, pp. 2235-2240, 1990.
 G. A. Ferreira, A. C. M. Magliano, E. M. F. Pral, and S. C. Alfieri, "Elastase secretion in Acanthamoeba polyphaga," Acta Tropica, vol. 112, no. 2, pp. 156-163, 2009.
 H. H. Kong, T. H. Kim, and D. I. Chung, "Purification and characterization of a secretory serine proteinase of Acanthamoeba healyi isolated from GAE," Journal of Parasitology, vol. 86, no. 1, pp. 12-17, 2000.
 W. T. Kim, H. H. Kong, Y. R. Ha et al., "Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence, "The Korean Journal of Parasitology, vol. 44, no. 4, pp. 321-330, 2006.
 A. J. Martinez, S. M. Markowitz, and R. J. Duma, "Experimental pneumonitis and encephalitis caused by Acanthamoeba in mice: pathogenesis and ultrastructural features," Journal of Infectious Diseases, vol. 31, no. 6, pp. 692-699, 1975.
 A. Martinez, "Free-living amoeba: natural history, prevention, diagnostic, pathology and treatment of disease," p. 156, CRC Press, Boca Raton, Fla, USA, 1985.
 A. J. Martinez, "Infection of the central nervous system due to Acanthamoeba," Reviews of Infectious Diseases, vol. 13, no. 5, pp. S399-S402, 1991.
 N. A. Khan, "Acanthamoeba invasion of the central nervous system," International Journal for Parasitology, vol. 37, no. 2, pp. 131-138, 2007.
 S. Alsam, J. Sissons, S. Jayasekera, and N. A. Khan, "Extracellular proteases of Acanthamoeba castellanii (encephalitis isolate belonging to T1 genotype) contribute to increased permeability in an in vitro model of the human blood-brain barrier," Journal of Infection, vol. 51, no. 2, pp. 150-156, 2005.
 N. A. Khan and R. Siddiqui, "Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins," International Journal for Parasitology, vol. 39, no. 14, pp. 1611-1616, 2009.
 J. F. De Jonckheere, "Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri," Infection, Genetics and Evolution, vol. 11, no. 7, pp. 1520-1528, 2011.
 F. Marciano-Cabral, "Biology of Naegleria spp," Microbiological Reviews, vol. 52, no. 1, pp. 114-133, 1988.
 I. Cervantes-Sandoval, J. D. J. Serrano-Luna, E. Garcia-Latorre, V. Tsutsumi, and M. Shibayama, "Characterization of brain inflammation during primary amoebic meningoencephalitis," Parasitology International, vol. 57, no. 3, pp. 307-313, 2008.
 K. L. Jarolim, J. K. McCosh, M. J. Howard, and D. T. John, "A light microscopy study of the migration of Naegleria fowleri from the nasal submucosa to the central nervous system during the early stage of primary amebic meningoencephalitis in mice," Journal of Parasitology, vol. 86, no. 1, pp. 50-55, 2000.
 K. L. Han, H. J. Lee, H. S. Myeong, H. J. Shin, K. I. Im, and S. J. Park, "The involvement of an integrin-like protein and protein kinase C in amoebic adhesion to fibronectin and amoebic cytotoxicity," Parasitology Research, vol. 94, no. 1, pp. 53-60, 2004.
 R. Herbst, C. Ott, T. Jacobs, T. Marti, F. Marciano-Cabral, and M. Leippe, "Pore-forming polypeptides of the pathogenic protozoon Naegleria fowleri," Journal of Biological Chemistry, vol. 277, no. 25, pp. 22353-22360, 2002.
 J. D. E. Young and D. M. Lowrey, "Biochemical and functional characterization of a membrane-associated pore-forming protein from the pathogenic ameboflagellate Naegleria fowleri," Journal of Biological Chemistry, vol. 264, no. 2, pp. 1077-1083, 1989.
 S. E. Barbour and F. Marciano-Cabral, "Naegleria fowleri amoebae express amembrane-associated calcium-independent phospholipase A2," Biochimica et Biophysica Acta, vol. 1530, no. 2-3, pp. 123-133, 2001.
 D. E. Fulford and F. Marciano-Cabral, "Cytolytic activity of Naegleria fowleri cell-free extract," Journal of Protozoology, vol. 33, no. 4, pp. 498-502, 1986.
 M. Shibayama, J. D. J. Serrano-Luna, S. Rojas-Hernandez, R. Campos-Rodriguez, and V. Tsutsumi, "Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I," Canadian Journal of Microbiology, vol. 49, no. 3, pp. 164-170, 2003.
 I. Cervantes-Sandoval, J. Jesus Serrano-Luna, J. Pacheco-Yepez, A. Silva-Olivares, V. Tsutsumi, and M. Shibayama, "Differences between Naegleria fowleri and Naegleria gruberi in expression of mannose and fucose glycoconjugates," Parasitology Research, vol. 106, no. 3, pp. 695-701, 2010.
 M. Jamerson, B. da Rocha-Azevedo, G. A. Cabral, and F. Marciano-Cabral, "Pathogenic Naegleria fowleri and nonpathogenic Naegleria lovaniensis exhibit differential adhesion to, and invasion of, extracellularmatrix proteins," Microbiology, vol. 158, pp. 791-803, 2012.
 F. L. Schuster and G. S. Visvesvara, "Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals," International Journal for Parasitology, vol. 34, no. 9, pp. 1001-1027, 2004.
 B. A. Rideout, C. H. Gardiner, I. H. Stalis, J.R. Zuba, T. Hadfield, and G. S. Visvesvara, "Fatal infections with Balamuthia mandrillaris (a free-living amoeba) in gorillas and other old world primates," Veterinary Pathology, vol. 34, no. 1, pp. 15-22, 1997.
 T. H. Dunnebacke, F. L. Schuster, S. Yagi, and G. C. Booton, "Balamuthia mandrillaris from soil samples," Microbiology, vol. 150, no. 9, pp. 2837-2842, 2004.
 A. Matin and N. A. Khan, "Demonstration and partial characterization of ecto-ATPase in Balamuthia mandrillaris and its possible role in the host-cell interactions," Letters in Applied Microbiology, vol. 47, no. 4, pp. 348-354, 2008.
 A. Martinez and G. S. Visvesvara, "Balamuthia mandrillaris infection," Journal of Medical Microbiology, vol. 50, no. 3, pp. 205-207, 2001.
 K. Janitschke, A. J. Martinez, G. S. Visvesvara, and F. Schuster, "Animal model Balamuthia mandrillaris CNS infection: contrast and comparison in immunodeficient and immunocompetent mice: a murine model of "granulomatous" amebic encephalitis," Journal of Neuropathology and Experimental Neurology, vol. 55, no. 7, pp. 815-821, 1996.
 A. F. Kiderlen and U. Laube, "Balamuthia mandrillaris, an opportunistic agent of granulomatous amebic encephalitis, infects the brain via the olfactory nerve pathway," Parasitology Research, vol. 94, no. 1, pp. 49-52, 2004.
 F. L. Schuster, T. H. Dunnebacke, G. C. Booton et al., "Environmental isolation of Balamuthia mandrillaris associated with a case of amebic encephalitis," Journal of Clinical Microbiology, vol. 41, no. 7, pp. 3175-3180, 2003.
 F. L. Schuster and G. S. Visvesvara, "Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals," Journal of Clinical Microbiology, vol. 34, no. 2, pp. 385-388, 1996.
 S. Jayasekera, A. Matin, J. Sissons, A. H. Maghsood, and N. A. Khan, "Balamuthia mandrillaris stimulates interleukin6 release in primary human brain microvascular endothelial cells via a phosphatidylinositol 3-kinase-dependent pathway," Microbes and Infection, vol. 7, no. 13, pp. 1345-1351, 2005.
 B. Rocha-Azevedo, M. Jamerson, G. A. Cabral, F. C. Silva-Filho, and F. Marciano-Cabral, "The interaction between the amoeba Balamuthia mandrillaris and extracellular matrix glycoproteins in vitro," Parasitology, vol. 134, no. 1, pp. 51-58, 2007.
Jesus Serrano-Luna, Carolina Pina-Vazquez, Magda Reyes-Lopez, Guillermo Ortiz-Estrada, and Mireya de la Garza
Departamento de Biologia Celular, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Avenida Instituto Politecnico Nacional 2508, 07360 Mexico, DF, Mexico
Correspondence should be addressed to Mireya de la Garza; email@example.com
Received 25 September 2012; Accepted 28 November 2012
Academic Editor: Carlos E. P. Corbett
TABLE 1: Cysteine proteases of Entamoeba histolytica that degrade host proteins and their implication in virulence. Tissue Purified Name Mr (kDa) or protein ECM components Yes EhCP1 27 Yes EhCP2 26 Yes EhCP112 35.5 Collagen Yes EhCP5 30 Yes -- 56 No -- 72 Yes -- 27-29 Yes EhCP4 26 Laminin Yes -- 56 Yes -- 27-29 Yes EhCP112 35.5 Fibronectin Yes -- 56 Yes -- 27-29 Gelatin No -- 110, 68, 56, and 22 Immunoglobulins IgA Yes EhCP4 26 Yes -- 70 Yes EhCP5 30 IgG Yes EhCP4 26 Yes EhCP1 27 Yes -- 56 Complement Yes EhCP4 26 C3 Yes EhCP1 27 Yes -- 56 C3a and C5a Yes -- 56 Cytokines Yes EhCP4 26 proIL-18 Yes EhCP5 30 Yes EhCP1 27 proIL-1[beta] Yes EhCP1 27 EhCP2 26 Iron-containing proteins Yes EhCP112 35.5 Yes EhCP5 30 Yes -- 41, 27 Hemoglobin No -- 32, 40 Yes -- 22 Yes -- 16 No -- 116, 82, 28, and 21 No 130, 43, 20, and 6 Transferrin No -- 130, 70, 50, 35, and 30 Lactoferrin No 250, 100, 40, and 22 Ferritin No -- 100, 75, and 50 Other proteins or tissues Mucin Yes EhCP5 30 Proteoglycan Yes EhCP2 26 Villin Yes EhCP1 27 Yes EhCP4 26 Fibrinogen Yes EhCP5 30 BSA Yes EhCP5 30 Tissue Purified Comment, localization Reference or protein ECM components Yes In vitro, surface  Yes In vitro, membrane-associated  Yes In vitro (zymogram), surface  Collagen Yes In vitro (zymogram), and it is [9,10] essential ex vivo for the cleavage of the collagen network, surface Yes In vitro, secreted  No In vitro (zymogram), crude  extracts and EDG Yes In vitro  Yes In vitro, secreted and nuclear  Laminin Yes In vitro, secreted  Yes In vitro  Yes In vitro (zymogram), surface  Fibronectin Yes In vitro, secreted  Yes In vitro  Gelatin No Induced by collagen,  conditioned medium Immunoglobulins IgA Yes In vitro, secreted and nuclear 14] Yes In vitro  Yes In vitro, surface  IgG Yes In vitro,secreted and nuclear 14] Yes In vitro, surface  Yes In vitro, secreted  Complement Yes In vitro, secreted and nuclear 14] C3 Yes In vitro, surface  Yes In vitro  C3a and C5a Yes In vitro  Cytokines Yes In vitro, secreted and nuclear 14] proIL-18 Yes In vitro, surface  Yes In vitro, surface  proIL-1[beta] Yes In vitro, surface [21, 22] In vitro, membrane-associated [21, 22] Iron-containing proteins Yes In vitro (zymogram), surface  Yes In vitro (zymogram), surface  Yes In vitro, intracellular  Hemoglobin No In vitro, total extract  Yes In vitro  Yes In vitro  No Human, porcine, and bovine  Hb in vitro (zymogram) No Total extract  in vitro (zymogram) Transferrin No Conditioned medium in vitro  (zymogram) Lactoferrin No Total extract  in vitro (zymogram) Ferritin No Total extract in vitro  (zymogram) Other proteins or tissues Mucin Yes In vitro (zymogram), although [9, 31] ex vivo is not needed to cross the mucus, surface Proteoglycan Yes In vitro, membrane-associated  Villin Yes In vitro, surface  Yes Iin vitro,secretedandnuclear  Fibrinogen Yes In vitro (zymogram), surface  BSA Yes In vitro (zymogram), surface  EDG: electron-dense granules. TABLE 2: Enzymes from E. histolytica related species that degrade host proteins and their implication in virulence. Parasite Tissue or Name Mr (kDa) protein E. moshkovskii ECM components -- [approximately equal to] 45 FIC strain Gelatin -- 90 Laredo strain Gelatin -- 90 E. dispar ECM components EdCP3 [approximately equal to] 30 E. invadens PZ strain Gelatin 99,90 IP101 strain Gelatin 99, 90, 45 IP-1 strain Gelatin 56, 58-66, 44-54, 43 Gelatin 45 Gelatin 130-230, 55, 35 Gelatin 130-230, 60 Azocasein 28 Parasite Tissue or Catalytic Comment protein type E. moshkovskii ECM components CP Zymogram and activity of proteases secreted in azocasein FIC strain Gelatin CP Zymogram Laredo strain Gelatin CP Zymogram E. dispar ECM components CP Sequence homology with E. histolytica. Located in cytoplasmic granules E. invadens PZ strain Gelatin CP Zymogram IP101 strain Gelatin CP Zymogram IP-1 strain Gelatin CP Zymogram Gelatin SP Zymogram Gelatin CP, MP Zymogram Gelatin CP, MP Zymogram Azocasein CP Zymogram Parasite Tissue or Reference protein E. moshkovskii ECM components  FIC strain Gelatin  Laredo strain Gelatin  E. dispar ECM components [34, 35] E. invadens PZ strain Gelatin  IP101 strain Gelatin  IP-1 strain Gelatin  Gelatin  Gelatin  Gelatin  Azocasein  TABLE 3: Enzymes from free-living amoebae (a) that degrade host proteins and their implication in virulence. Pathogen Tissue or protein Purified Name Acanthamoeba Elastin No spp. Collagen, Yes fibronectin, and laminin Corneal stroma Yes MIP-133 (ex vivo), Collagen I and IV Acanthamoeba Corneal stroma Yes MIP-133 castellanii (ex vivo), Collagen Collagen I and III No Elastin Collagen I and III No Collagen I and IV Fibronectin, Yes hemoglobin, albumin, IgA, and IgG Gelatin No Gelatin No Acanthamoeba Gelatin No polyphaga Gelatin No Acanthamoeba Collagen I and IV Yes healyi Fibronectin, hemoglobin, albumin, IgA, and IgG Acanthamoeba Collagen I and IV Yes lugdunensis Fibronectin, hemoglobin, albumin, IgA, and IgG Collagen I and, Yes elastin Bovine mucin No Naegleria Gelatin No fowleri Gelatin No Balamuthia Collagen I and III, No mandrillaris elastin, and plasminogen Collagen I and III, No elastin, and plasminogen Pathogen Tissue or protein Mr (kDa) Catalytic type Acanthamoeba Elastin 70-130 SP spp. Collagen, 12 SP fibronectin, and laminin Corneal stroma 133 SP (ex vivo), Collagen I and IV Acanthamoeba Corneal stroma 133 SP castellanii (ex vivo), Collagen Collagen I and III 130 SP Elastin Collagen I and III 150 MMP Collagen I and IV Fibronectin, 33 178, 144, SP hemoglobin, albumin, IgA, and IgG Gelatin 123, 110, 78, SP 75, 72, 34, 30 Gelatin 188, 157, SP 53, 34 Acanthamoeba Gelatin 144, 105, SP 78, 72, 45, 40, 34 polyphaga Gelatin 72, 62, SP 50, 34 Acanthamoeba Collagen I and IV 33 SP healyi Fibronectin, hemoglobin, albumin, IgA, and IgG Acanthamoeba Collagen I and IV 33 SP lugdunensis Fibronectin, hemoglobin, albumin, IgA, and IgG Collagen I and, 30 CP elastin Bovine mucin 37 CP Naegleria Gelatin 130, 100, CP fowleri 73, 62 Gelatin 310, 178, CP 164, 147 Balamuthia Collagen I and III, 40-50 MMP mandrillaris elastin, and plasminogen Collagen I and III, 73 40-50 MMP elastin, and plasminogen Pathogen Tissue or protein Localization Reference Acanthamoeba Elastin Secreted  spp. Collagen, Secreted  fibronectin, and laminin Corneal stroma Secreted  (ex vivo), Collagen I and IV Acanthamoeba Corneal stroma Secreted  castellanii (ex vivo), Collagen Collagen I and III Secreted  Elastin Collagen I and III Secreted  Collagen I and IV Fibronectin, Secreted  hemoglobin, albumin, IgA, and IgG Gelatin Crude  extracts Gelatin Conditioned  medium Acanthamoeba Gelatin Crude  extracts polyphaga Gelatin Conditioned  medium Acanthamoeba Collagen I and IV Secreted  healyi Fibronectin, hemoglobin, albumin, IgA, and IgG Acanthamoeba Collagen I and IV Secreted  lugdunensis Fibronectin, hemoglobin, albumin, IgA, and IgG Collagen I and, Secreted and  elastin cytoplasmic Bovine mucin Crude  extracts Naegleria Gelatin Crude  fowleri extracts Gelatin Conditioned  medium Balamuthia Collagen I and III, Crude  mandrillaris elastin, and extracts plasminogen Collagen I and III, Conditioned  elastin, and medium plasminogen All results have been performed in in vitro assays. MIP: mannose induced protease; SP: serine protease; CP: cysteine protease; MMP: matrix metalloprotease.
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|Author:||Serrano-Luna, Jesus; Pina-Vazquez, Carolina; Reyes-Lopez, Magda; Ortiz-Estrada, Guillermo; de la Gar|
|Publication:||Journal of Tropical Medicine|
|Date:||Jan 1, 2013|
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