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Prevalence of giardiasis in children attending semi-urban daycare centres in Guatemala and comparison of 3 Giardia detection tests.

INTRODUCTION

Giardia intestinalis is an intestinal protozoan parasite widely prevalent in children aged less than 5 years but the long-term health consequences of giardiasis in children have not been fully elucidated (1,2). Giardiasis has been associated with lower serum zinc, iron, and vitamin A in children, despite similar anthropometric indicators among infected and non-infected individuals (3,4).

Giardiasis can be diagnosed through identification of cysts, trophozoites and Giardia-specific antigens in faecal samples. Several faecal tests are available. Comparison of faecal diagnostic methods is problematic due to the lack of a true gold standard reference method (5). The ovum and parasite (O&P) method is the currently-accepted gold standard method despite its inferior sensitivity compared to enzyme-linked immunosorbent assays (ELISA), particularly when only a single faecal sample is available for analysis (6).

The factor of intensity of Giardia infection, often not measured or reported, may have been a confounding factor in previous studies that have failed to identify a negative impact of Giardia infection on the anthropometric indicators of children. Intensity of infection (cysts/g of faeces) can be determined through light microscopy by cyst concentration procedures, followed by quantification using a haemocytometer. Alternatively, the antigen concentration as determined by spectrophotometer readings of an ELISA may offer a more standardized method of assessing intensity of infection.

The main objective of this study was to determine the prevalence of asymptomatic giardiasis in children attending daycare centres near Quetzaltenango, Guatemala, over a 5-week longitudinal component that allowed for detection of new infection, self-clearance, persistent infection, or no infection. The second objective was to compare the detection and quantification of the intensity of G. intestinalis infection, using light microscopy, ELISA, and a rapid dipstick test.

MATERIALS AND METHODS

Two stool samples per week from each subject were collected during Week 0 and Week 4 (i.e. a total of 4 samples per subject). Collection-kits were given to each parent on the day prior to the collection date, and instruction on how to use the kits was provided. Parents brought back the filled container the following morning, and the study staff transported those faecal samples to the nearby laboratory. Fresh faecal samples were analyzed for the presence of cysts in the faeces by microscopy, using the sucrose gradient concentration method and after staining with Lugol's solution (7). Intensity of Giardia infection was expressed as the number of cysts per gramme of faeces. A standardized scoop of faecal material was measured, and mass was recorded in grammes up to 2 decimals. Cysts were quantified using a haemocytometer (22 mm x 22 mm cover-slips, thickness 1) and a bright field microscope with high-power (40x) objective (7). Split faecal samples were also stored at -20[degrees]C and analyzed within 6 weeks for presence of G. intestinalis antigens in the faeces, using ELISA, or the dipstick test. The ProSpecT-Giardia-EZ-microplate assay (Oxoid, Cambridge, UK) was used in analyzing all faecal samples according to the manufacturer's instructions. Absorbance values were read using the co-analyzer 303+ with filters of 450 and 630 nm (Corporacion Analiticos, S.A., Guatemala City, Guatemala). The absorbance values from the ELISA ranged from 0.0 to a maximum of 3.0. A value [greater than or equal to] 0.05 was considered positive, meaning Giardia-specific antigen 65 was detected in the sample. RidaQuick Giardia dipstick test (R-Biopharm AG, Damstadt, Germany) was employed. The test was read as positive or negative (with a control indicator on the dipstick) within 5 minutes.

Ethics

The Human Research Ethics Review Board of the University of Alberta and the ZUGUEME Ethics Board of Guatemala City approved the study.

Statistical analysis

Information on height (to the nearest 0.5 cm) and weight (to the nearest 0.1 kg) for each subject was available at the time of the study. Data were analyzed using the Centers for Disease Control and Prevention (CDC) 2000 child growth standards to calculate z-scores of weight-for-height (WHZ), height-for-age (HAZ), and weight-for-age (WAZ) (8). Among the ELISA-positive subjects, the Spearman correlation value was calculated, with the absorbance values as the x-axis variables and the WAZ, HAZ, and WHZ values on the y-axis. One ELISA absorbance value per individual was used; if two positive samples were provided, the mean of both samples was used in the regression.

To compare the intensity of infection, the Spearman correlation value was determined between the number of cysts/g of faeces (based on microscopic analysis, positive samples only, n = 15) and the corresponding ELISA absorbance values. Statistical analysis was performed using GraphPad Prism (version 5.03) for Windows (GraphPad Software, San Diego, California, USA, www.graphpad.com).

RESULTS

Of the 53 children enrolled (aged 1.5-7 years), 48 children each provided at least 1 stool sample; 15 children provided at least one stool sample at both time-points and 23 children provided all 4 stool samples. All samples were analyzed by ELISA but only a subset of fresh samples (n = 75) were analyzed by a single researcher through microscope due to time limitations. The corresponding 75 samples were assessed by dipstick. Based on ELISA results, 43.8% of the subjects tested positive for giardial co-proantigen at Week 0. A similar prevalence (44.7%) was found at Week 4. The percentage of individuals with new infection, self-clearance, chronic infection or with no G. intestinalis infection is illustrated in Table 1 both for the cohort of children who each gave at least 1 stool sample at each end of the study (n = 15) and for the 4-stool cohort (n = 23), providing all 4 requisite stool samples. The 4-stool cohort appeared to exhibit a higher percentage of persisting infection compared to the subjects who provided 2 or 3 stool samples.

Diagnostic agreement among the 75 samples evaluated by all three detection methods was 74.7%. Using ELISA as the gold standard, the microscopic analysis had a slightly higher specificity and positive predictive value (PPV), indicating greater confidence in positive test results relative to the dipstick method (Table 2). The dipstick method had a higher sensitivity and negative predictive value (NPV), indicating greater confidence in negative test results relative to microscopic analysis.

Comparison of intensity of Giardia infection, using the microscopic cyst counts (positive samples only, n = 15) vs ELISA absorbance values indicates that all of the positive samples detected by microscopy had high ELISA absorbance values (>1.7), the majority (n = 14) having near-maximal absorbance values of >2.9). Alternatively, not all of the samples with high ELISA absorbance values (>1.7) were positive for G. intestinalis with microscopic examination. The range in cyst concentration detected by microscope was 2.000-358.750 x10 000 cysts/g of faeces. The Spearman correlation, using scaling values as proxies for intensity of infection between cyst count and absorbance reading, was nonsignificant (p = 0.26). The detection limit of the dipstick was 2.000 x 10 000 cysts/g and an ELISA OD value of 2.5.

Subjects with a higher ELISA coproantigen intensity, though not significant, tended to have lower WAZ (r = -0.32, 1-tailed p = 0.08). The 1-tailed test was performed after the general consistency of a negative coefficient correlation, i.e. an inverse association between intensity of infection and growth was shown across the board for all three indicators (WAZ, WHZ, and HAZ). No significant correlations were found between ELISA coproantigen intensity and HAZ (p = 0.12), or WHZ (p = 0.36).

DISCUSSION

The prevalence of giardiasis was high in the subjects examined in this study. Our findings are consistent with the literature, in which the higher detection of giardiasis was reported when a second sample was provided (9). The second stool sample detected an additional 7 cases of giardiasis. It is possible that there were undetected cases of persistent infection when only a single stool sample per week was collected. The reported prevalence rate may, therefore, be a conservative estimate.

In comparison with the microscopic and dipstick methods, the ELISA method detected almost twice as many cases of giardiasis (Table 3). The reported sensitivity of different microscopic methods for single stool analysis has ranged between 65% and 75% (9,10). The sensitivity of microscopy in our study is lower at 53.6%. The sensitivity of RidaQuick dipsticks reported in the literature is 80%, with specificity of 99.4% (10). The sensitivity in our study is lower (60.7%), with specificity comparable (97.9%) to what has been reported in the other literature (5). The lower sensitivities (microscopy and dipstick) observed in our study could be due to low faecal cyst concentrations. Asymptomatic carriers were found to have lower cyst counts (11) compared to symptomatic cases. Dipsticks consistently detected cases having the highest intensity of infection and could be useful for rapid screening and remote fieldwork settings.

Previous studies have found associations between ELISA intensity values and Giardia cyst concentration (12,13). In this study, there was no significant correlation between cyst count using microscopic analysis and ELISA OD values. This could be due to a lack of spread in the ELISA absorbance values; 93% of the 15 samples had an absorbance value of 2.9 to 3.0, indicating maximal or near-maximal absorbance. A limitation of using ELISA as a quantitative method is the maximal absorbance level of 3.0. This level is likely too low; a higher ceiling would be necessary for the cases of giardiasis with a higher intensity of infection. Further systematic dilution of samples could provide adjusted absorbance readings above the current 3.0 limit. Standardized test to determine the correlation between expanded ELISA OD and the full range of faecal cyst counts would be the next opportunity to be pursued.

ACKNOWLEDGEMENTS

For their technical guidance and assistance, we extend our thanks to: Lic. Gloria Hidalgo Rivas, MSc student (biological laboratory La Democracia, Quetzaltenango, Guatemala); Dr. Al Shostak (Department of Biological Sciences, University of Alberta); Joy Nolte, MSc student (Boston University); Dr. Maria Jose Rios (CeSSIAM); Claudia Arriaga Godoy (CeSSIAM); and Lic. Lillian de la Cruz (Regional Supervisor, Secretaria de las Obras Sociales de la Esposa del Presidente, Guatemala).

REFERENCES

(1.) Hollm-Delgado M-G, Gilman RH, Bern C, Cabrera L, Sterling CR, Black RE et al. Lack of an adverse effect of Giardia intestinalis infection on the health of Peruvian children. Am J Epidemiol 2008;168:647-55.

(2.) Carvalho-Costa FA, Goncalves AQ Lassance SL, da Silva Neto LM, Salmazo CAA, Boia MN. Giardia lamblia and other intestinal parasitic infections and their relationships with nutritional status in children in Brazilian Amazon. Rev Inst Med Trop Sao Paulo 2007;49:147-53.

(3.) Demirci M, Delibas N, Altuntas I, Oktem F, Yonden Z. Serum iron, zinc and copper levels and lipid peroxidation in children with chronic giardiasis. J Health Popul Nutr 2003;21:72-5.

(4.) Quihui-Cota L, Astiazaran-Garcia H, Valencia ME, Morales-Figueroa GG, Lopez-Mata MA, Vazquez Ortiz F. Impact of Giardia intestinalis on vitamin a status in schoolchildren from northwest Mexico. Int J Vitam NutrRes 2008;78:51-6.

(5.) Weitzel T, Dittrich S, Mohl I, Adusu E, Jelinek T. Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples. Clin Microbiol Infect 2006;12:656-9.

(6.) Hanson KL, Cartwright CP. Use of an enzyme immunoassay does not eliminate the need to analyze multiple stool specimens for sensitive detection of Giardia lamblia. J Clin Microbiol 2001;39:474-7.

(7.) Daniels CW, Belosevic M. Disaccharidase activity in male and female C57BL/6 mice infected with Giardia muris. Parasitol Res 1995;81:143-7.

(8.) Kuczmarski RJ, Ogden CL, Guo SS, Grummer-Strawn LM, Flegal KM, Mei Z et al. 2000 CDC growth charts for the United States: methods and development. Vital Health Stat 11 2002;(246):1-190.

(9.) Hiatt RA, Markell EK, Ng E. How many stool examinations are necessary to detect pathogenic intestinal protozoa? Am J Trop Med Hyg 1995;53:36-9.

(10.) Oster N, Gehrig-Feistel H, Jung H, Kammer J, McLean JE, Lanzer M. Evaluation of the immunochromatographic CORIS Giardia-Strip test for rapid diagnosis of Giardia lamblia. Eur J Clin Microbiol Infect Dis 2006;25:112-5.

(11.) Soliman MM, Taghi-Kilani R, Abou-Shady AFA, El-Mageid SAA, Handousa AA, Hegazi MM et al. Comparison of serum antibody responses to Giardia lamblia of symptomatic and asymptomatic patients. Am J Trop Med Hyg 1998;58:232-9.

(12.) Vidal AMB, Catapani WR. Enzyme-linked immunosorbent assay (ELISA) immunoassaying versus microscopy: advantages and drawbacks for diagnosing giardiasis. Sao Paulo Med J 2005;123:282-5.

(13.) Addiss DG, Mathews HM, Stewart JM, Wahlquist SP, Williams RM, Finton RJ et al. Evaluation of a commercially available enzyme-linked immunosorbent assay for Giardia lamblia antigen in stool. J Clin Microbiol 1991;29:1137-42.

Terri-Lynn Duffy [1], Gabriela Montenegro-Bethancourt [2], Noel W. Solomons [2], Miodrag Belosevic [3], M. Thomas Clandinin [1]

[1] Department of Agriculture, Food and Nutritional Science, University of Alberta, Canada; [2] Center for Studies of Sensory Impairment, Aging, and Metabolism, Guatemala; [3] Department of Biological Sciences, University of Alberta, Canada

Correspondence and reprint requests:

Terri-Lynn Duffy

University of Alberta

Edmonton, Alberta

Canada, T6G-2N8

Email: tduffy@ualberta.ca
Table 1. Percentage of sample subjects displaying
new infection, self-clearance, persistent
infection, and no infection with
G. intestinalis over a 5-week period

Infection status       * 4-stool   ([dagger]) Remaining
                        cohort           subjects
                       (n = 23)       (n = 15) % (n)
                         % (n)

New infection           8.7 (2)          26.7 (4)
Self-clearance          8.7 (2)          13.3 (2)
Persistent infection   34.8 (8)          20.0 (3)
No infection           47.8 (11)         40.0 (6)

* Subjects who submitted all 4 stool samples (n = 23).

([dagger]) All remaining subjects who submitted at
least 1 stool sample at each time-point (n = 15)

Table 2. Sensitivity, specificity, PPV *, and NPV
([dagger]) of microscope and dipstick test for
Giardia detection in stool samples, using
ELISA as gold standard

Parameter     Microscope   Dipstick
               (n = 75)    (n = 75)

Sensitivity      53.6        60.7
Specificity     100.0        97.9
PPV             100.0        94.4
NPV              78.3        80.7

* PPV = Positive predictive value; ([dagger]) NPV
= Negative predictive value

Table 3. Summary of Giardia detection in faecal
samples from ELISA, dipstick and microscopic analysis

Nature of             ELISA     Dipstick   Microscope
samples              (n = 75)   (n = 75)    (n = 75)

Number of positive
  samples               28         18          15
Number of negative
  samples               47         57          60
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Article Details
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Title Annotation:SHORT REPORT
Author:Duffy, Terri-Lynn; Montenegro-Bethancourt, Gabriela; Solomons, Noel W.; Belosevic, Miodrag; Clandini
Publication:Journal of Health Population and Nutrition
Article Type:Report
Geographic Code:2GUAT
Date:Jun 1, 2013
Words:2346
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