Prevalence of Aflatoxin in feeds and cow milk from five counties in Kenya.
Kenya's dairy industry is a major source of livelihood for farmers, milk processors, milk traders, feed manufacturers and feed retailers. The industry contributes 14% of the agricultural gross domestic product (GDP) and 3.5% of the total GDP . Milk production in Kenya is mainly from cattle; camels and goats contribute to a lesser extent . Dairy cattle breeds produce 70% of milk. The number of dairy cattle was estimated at 3.6 million in 2007 by the Government of Kenya . In 2007, the former Rift Valley Province was estimated to have 53% of the dairy cattle population, while former Central had 24%, Eastern had 8%, Nyanza had 6%, Western had 5%, Coast had 3% and Nairobi 1% .
The Kenya dairy production system consists of a mix of small-scale dairy farming and large-scale dairy production, but small-scale dairy farming predominates producing about 80% of the milk in the country . There is a higher concentration of smallholder dairy farms in peri-urban areas where there is easy access to marketing channels for high-priced unpasteurized and pasteurized milk. Large dairy farms are owned by public institutions and companies as well as individuals . Smallholder dairy production usually involves stall-feeding of fodder and grass (zero-grazing) supplemented with homemade or purchased concentrate feed . Large-scale dairy production in Kenya mainly utilises pastures with little concentrate feed. Kenya has a high milk consumption: one estimate suggests consumption of 145 litres per capita per year, which is over three times the consumption in other East African countries . However, the average milk production per dairy cow per year is low, 2920 kg in Kenya, compared to 4590 kg in South Africa and 10,096 kg in the United States of America [7-9].
Food safety is important in both developed and developing countries. In Kenya, milk is liable to contamination with hazards including aflatoxins. Aflatoxins are mycotoxins produced mainly by Aspergillus flavus and A. parasiticus moulds. The major aflatoxins are B1, B2, G1 and G2 . Aflatoxin M1 (AFM1) and M2, the hydroxylated products of B1 and B2, are found in milk and milk products. Aflatoxin B1 (AFB1) is considered a class 1 carcinogen and can cause acute and chronic illness in people and animals . Aflatoxin M1 is a class 2B (possible) human carcinogen.
Maize, the staple food in Kenya, is often contaminated with high concentrations of aflatoxins and this has caused acute fatal aflatoxicosis in humans [11-17]. High concentrations of aflatoxins and trichothecenes in feed can also cause high mortality in cattle , while chronic aflatoxin poisoning in dairy cattle leads to a decrease in feed conversion efficiency, milk production and reproductive efficiency [19, 20]. There have been many studies on aflatoxins in crop products in Kenya [11-17, 21, 22], but less attention has been paid to aflatoxins in dairy products. In Kenya, studies on the prevalence of AFB1 in dairy feeds and AFM1 in milk were mainly conducted in urban and peri-urban areas [23-25]. The present study tries to fill this gap by assessing aflatoxin contamination and prevalence in dairy feeds and milk in all milk-producing agroecological zones (AEZs) in Kenya.
Study site selection
A map showing AEZs of Kenya  was used to select the study sites. The counties in each AEZ were listed and one study site each was randomly selected from the sub-humid, humid and semi-arid zones; two study sites were selected from the temperate zone, as this zone is favourable for dairy keeping. The arid zone was not sampled as it is not favourable for dairy breeds. The randomly selected counties were Kisii and Bungoma (temperate), Tharaka-Nithi (humid), Kwale (sub-humid) and Isiolo (semi-arid) (Figure 1). One sub-location was randomly selected from each county.
[FIGURE 1 OMITTED]
Multistage cluster sampling was used, with sub-locations, then villages and then dairy farmers randomly selected using computer-generated random numbers . To do this, sampling frames were constructed of sub-locations, villages within the selected sublocations and then farmers with at least one milking cow within the villages. Eight villages were selected from each sampling sub-location, and in each village, eight eligible farmers were randomly selected. Sampling was planned to coincide with the dry (February and March 2014) and rainy (July and October 2014) seasons. Aflatoxin production is higher in feeds stored in damp conditions and this study sought to investigate the effect of season on aflatoxin outcome of the feeds and milk.
To get representative samples, bulk household milk (300 ml) was taken and feed (500 g) scooped at three levels from a bag (top, middle and bottom). To sample the feed, a scoop sterilised in sodium hypochlorite was used. The farmers gave the location of feed retailers from where they purchased feeds. All feed retailers in the local market were sampled. In addition, feed samples were obtained from the manufacturers who supplied the retailers, using the sampling method described earlier. One sample of each type of dairy feed available was taken from the farmer, feed retailer and feed manufacturer. In Isiolo County, there were no feed retailers and none of the farmers fed their cattle on concentrates. Feed samples were transported in boxes and kept in a cold room at 4[degrees]C. Milk samples were frozen at -20[degrees]C. Analysis was carried out at the Department of Public Health, Pharmacology and Toxicology, University of Nairobi and the Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub. Ethical approval for the study was acquired from the International Livestock Research Institute (approval number ILRI-IREC2013-09). In addition, a short questionnaire was completed for each farm.
Aflatoxin B1 analysis in feed
Aflatoxin B1 analysis of the feeds was done using a low matrix competitive AFB1 enzyme-linked immunosorbent assay (ELISA) kit for cereals and grains (Helica Biosystems, Inc., Santa Ana, CA 92704, USA, Catalog No. 981BAFL01LM-96 Low Matrix), according to the manufacturer's instructions. Calf pellets with a large particle size were ground using a Romer grinder (Romer Series II Mill from Romer Labs Inc., 1301 Stylemaster Drive Union, MO 63084, USA). Samples with AFB1 values above the highest standard concentration were further diluted and the assay conducted again until the AFB1 value quantified fell between the lowest and the highest aflatoxin values in the standards. The limit of detection for AFB1 was one part per billion.
Aflatoxin Ml analysis
Quantification of AFM1 was done using a commercial Helica[R] AFM1 ELISA quantitative kit (Helica Biosystems, Inc., Santa Ana, CA 92704, USA, Catalog No. 961AFLM01M-96) according to the manufacturer's instructions. Samples with AFM1 values above the highest standard concentration were further diluted and the assay conducted again until the AFM1 value quantified fell between the lowest and the highest aflatoxin values in the standards. The limit of detection of AFM1 was two parts per trillion (ppt).
Data were entered into Microsoft Excel 2010 and exported to Stata Version 13. The data were analysed using descriptive statistics. The AFB1 and AFM1 concentrations in feeds and milk did not follow the normal distribution. In calculation of geometric means, values lower than the limit of detection of AFB1 and AFM1 were replaced by half the value of the limit of detection for the respective kits to avoid biasing the results. Two-sample Wilcoxon rank-sum (Mann-Whitney) test was used for analysis of variance of median aflatoxin levels among counties and AEZs. Wilcoxon sign-rank test was used to evaluate difference between households that were sampled during both seasons.
Cattle breeds kept and milk production
A total of 285 households were surveyed. Cattle breeds kept included Friesian, Ayrshire, Guernsey and Jersey (dairy breeds), cross breeds and local breeds (Table 1). Farmers in Kisii and Tharaka-Nithi counties had a higher proportion of dairy breeds and a larger proportion of households fed dairy concentrates to cattle. Daily milk production per cow varied from 0.15 litres for local breed cattle in a farm in Isiolo to 29 litres for dairy cattle in a farm in Kwale and mean daily milk production per cow was highest in Kisii (Table 2). All calves in Isiolo and Kwale counties suckled from their dams. Tharaka-Nithi had the highest proportion of farmers feeding the calves on milk (20/64), followed by Kisii (10/64) and Bungoma (3/64). The amount of milk fed to calves varied from 0.5 to seven litres per calf per day.
A total of 285 households provided samples: 37 in Kwale, 56 in Isiolo, 64 in Tharaka-Nithi, 64 in Kisii and 64 in Bungoma. A total of 512 milk samples were obtained from the households. Two hundred and seventy seven feed samples were collected from households (n=144), feed retailers (n=31) and feed manufacturers (n=102). Feed manufacturers were from the counties of Mombasa (supplies Kwale County), Meru (supplies Tharaka-Nithi County), Bungoma (supplies Bungoma County) and Nakuru (supplies Kisii and Bungoma counties). The dairy feeds consisted of dairy meal, pollard, maize, maize germ, maize bran, rice germ, rice bran, wheat pollard, wheat bran, young stock, calf meal, calf pellet, sorghum, cotton seed, sunflower and pyrethrum mix and home-made concentrates.
Aflatoxin B1 in feeds from feed manufacturers, feed retailers and farmers
Geometric means of AFB1 in feeds from feed manufacturers, feed retailers and farmers were 9.8 parts per billion (ppb), 25.6 ppb and 13.7 ppb, respectively (Table 3, 4 and 5). All feeds from feed manufacturers from Meru County had AFB1 levels above the World Health Organization/Food and Agriculture Organization of the United Nations (WHO/FAO) limit of 5 ppb (Table 3). Aflatoxin B1 (AFB1) concentration in farmers' feeds (geometric means) was highest in Tharaka-Nithi (Table 5). Home produced dairy feeds had lower AFB1 geometric means (0.4 ppb in the dry season, n=18; 18.9 ppb in the rainy season, n=4) than purchased feeds (7.0 ppb in the dry season, n=41; 25.3 ppb in the rainy season, n=20). In Tharaka-Nithi County (humid AEZ), the rainy season AFB1 concentration in farmers' feeds was higher than that of the dry season as shown by the Wilcoxon rank-sum test at 95% level of confidence (Table 6). Prevalence of AFB1 in farmers' feeds is shown in Figure 2. In the dry season, Bungoma County had the highest AFB1 levels with 25% of the samples having concentrations above 55 ppb, followed by Kisii County with 25% of the samples above 40 ppb. In the rainy season, Tharaka-Nithi County had many feed samples with high AFB1 concentration, up to 9661 ppb. In Isiolo County, there was rain failure in July 2014, so no feed samples were available as farmers did not use dairy feed.
[FIGURE 2 OMITTED]
Aflatoxin M1 in milk
In total, 512 samples from 282 farmers were analysed and 39.7% of these had levels above the limit of detection, and 10.4% exceeded 50 ppt. Tharaka-Nithi County (humid AEZ) had the highest proportion of milk samples (26.2%) with AFM1 concentrations above the WHO/FAO limit of 50 ppt (Table 7). Milk samples from Isiolo County had higher AFM1 levels in the July dry season than the February dry season (p=0.02, Table 8). The Wilcoxon sign-rank test for Bungoma County showed the AFM1 milk concentration was higher in the dry season than the rainy season (p<0.001, Table 8). The distribution of milk samples with AFM1 is shown in Figure 3. In the dry season, Kwale County had the highest median (>200 ppt AFM1 in milk), followed by Kisii County with an AFM1 median above 100 ppt. During the rainy season, Tharaka-Nithi and Kisii counties had milk AFM1 values above 900 ppt and 400 ppt, respectively.
[FIGURE 3 OMITTED]
This study describes levels of aflatoxins present in cattle feed and cattle milk, assessed in one year in Kenya. The high prevalence and concentration of AFB1 in dairy feeds and AFM1 in cattle milk from rural villages and urban centres reported in this study are comparable to earlier reports in Kenya from urban and peri-urban areas [23, 24] but higher than those reported from Ethiopia . The geometric mean AFB1 in feed from farmers is lower than that in feed from feed retailers, possibly due to lower initial aflatoxin contamination of home-made feeds or poor storage practices of the manufactured feeds along the dairy feed value chain. The higher AFB1 concentration in feed from feed retailers compared to that in feed from feed manufacturers suggests contamination or multiplication of Aspergillus fungi along the dairy feed chain. Tharaka-Nithi and Kisii counties had a higher proportion of dairy breeds and a corresponding higher proportion of farmers who fed dairy concentrates to cattle. This led to higher proportions of milk from the two counties exceeding the 50 ppt AFM1 WHO/FAO limit.
Feed aflatoxin concentrations above 100 ppb were recorded in Tharaka-Nithi County and this may be due to the presence of high-aflatoxin-producing Aspergillus strains in this region  and/or feed storage conditions that favour the multiplication of Aspergillus fungi. High AFB1 concentrations in dairy feeds have been shown to reduce milk production by up to 25%  and decrease in feed conversion efficiency and reproduction efficiency .
The low AFM1 concentrations in Bungoma during the rainy season may be due to availability of natural pastures and low use of dairy concentrates. Isiolo County was dry in July and dairy concentrates were not fed to the cattle. However, there was rain in the neighbouring counties of Laikipia and Meru. This may have raised the relative humidity of the environment leading to higher water activity in the dry pastures, which may have facilitated multiplication of Aspergillus fungus and higher AFM1 levels in milk during the July 2014 dry season.
Most of the feed from feed manufacturers analysed had AFB1 levels above the WHO/FAO and Kenyan standards [30, 31]. On the part of the government, there is a need to educate and supervise the farmers, feed traders and feed manufacturers on the importance of producing crops and feeds with low levels of, or exempt from, aflatoxin and observing good feed storage practices especially during the rainy season. In addition to providing important and novel information on aflatoxins in milk, this study shows that aflatoxin contamination is common in dairy feeds and milk and concentrations may be high. This may contribute to ill health effects in both humans and animals. Therefore, there is need for better understanding of the impacts of aflatoxins in the dairy and feed value chains and, where appropriate, interventions within these value chains to control aflatoxin contamination in animal feeds. Research can help identify the factors that contribute to aflatoxin contamination of feeds at the feed manufacturing plants and along the dairy feed value chain.
This study was a part of the FoodAfrica Programme which is mainly financed by the Ministry for Foreign Affairs of Finland contract no. 29891501 (FoodAfrica) and the CGIAR Research Program on Agriculture for Nutrition and Health. The authors acknowledge the BecA-ILRI Hub mycotoxin laboratory for hosting the laboratory work, and Helica for providing kits at lower costs. The authors thank the participating villages and sampled households for their co-operation.
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Senerwa DM (1, 2) *, Sirma AJ (1, 2), Mtimet N (1), Kang'ethe EK (2), Grace D (1) and JF Lindahl (1,3)
* Corresponding author email: firstname.lastname@example.org
(1) International Livestock Research Institute, P.O. Box 30709-00100, Nairobi, Kenya
(2) Department of Public Health, Pharmacology and Toxicology, University of Nairobi, P.O. Box 29053-00625, Nairobi, Kenya
(3) Department of Clinical Sciences, Swedish University of Agricultural Sciences, P.O. Box 7054, SE-750 07 Uppsala, Sweden
Table 1: Breed composition in study households and proportion providing dairy concentrate samples County Agro-ecological Number of cattle Dairy Cross zone in sampled breeds breeds households (%) (%) Kwale Sub-humid 409 2.9 13.0 Isiolo Semi-arid 492 0.0 0.0 Tharaka-Nithi Humid 153 32.7 48.4 Kisii Temperate 193 40.9 52.3 Bungoma Temperate 180 13.3 61.7 County Local PH-C (%) breeds (%) Kwale 84.1 5.4 (2/37) Isiolo 100.0 0.0 (0/56) Tharaka-Nithi 19.0 56.3 (36/64) Kisii 6.7 68.8 (44/64) Bungoma 25.0 21.9 (14/64) PH-C: proportion of households that provided dairy concentrate samples Table 2: Cow milk production in sampled households and calf feeding practices County Agro- Number of Milk Range ecological households production (litres) zone sampled (litres/cow/day) Kwale Sub-humid 32 2.35 0.25-29 Isiolo Semi-arid 55 0.56 0.15-2.1 Tharaka-Nithi Humid 62 3.47 0.3-10 Kisii Temperate 64 4.20 1.0 -18 Bungoma Temperate 63 2.98 0.3-12 County Standard Milk fed deviation (litres/calf/day) (litres) Kwale 4.98 NA Isiolo 0.43 NA Tharaka-Nithi 2.45 2.5 Kisii 3.07 3.5 Bungoma 2.30 1.4 NA: not applicable Table 3: Prevalence and levels of aflatoxin B1 in feeds from different feed manufacturers encountered in the study sites Feed Number Feed market- AEZ of Prevalence source- of feed County county where >5ppb (%) County samples feed is fed to cattle Mombasa 7 Kwale Sub-humid 28.6 Meru 9 Tharaka-Nithi Humid 100.0 Nakuru 76 Kisii, Bungoma Temperate 59.2 Bungoma 10 Bungoma Temperate 70.0 All 102 --- --- 61.8 Feed Range (ppb) A. mean Median( G. mean source- (ppb) ppb) (ppb) County Mombasa <1-51.7 9.8 2.9 2.8 Meru 14-4682 875.7 162.3 175.0 Nakuru <1-252.9 31.6 8.5 7.2 Bungoma <1-204.7 75.0 53.5 19.1 All <1-4682 108.9 11.7 9.8 A. mean: arithmetic mean; G. mean: geometric mean; one part per billion (ppb) is the limit of detection Table 4: Prevalence and levels of aflatoxin B1 in feeds from feed retailers in the study sites County Number of Prevalence Range (ppb) A. mean samples >5 ppb (%) (ppb) Tharaka-Nithi 15 86.7 <1-1198 115.3 Kisii 10 100.0 9-310 76.8 Bungoma 6 83.3 <1-103 47.1 All 31 90.3 <1-1198 89.7 County Median G. mean (ppb) (ppb) Tharaka-Nithi 20.3 19.1 Kisii 48.6 46.6 Bungoma 52.8 19.7 All 42.3 25.6 A. mean: arithmetic mean; G. mean: geometric mean; one part per billion (ppb) is the limit of detection Table 5: Prevalence of aflatoxin B1 in feeds obtained from farmers in the selected counties in Kenya County Agro-ecological Number Prev. Prev. Prev. zone of feed >1ppb >5ppb >10ppb samples (%) (%) (%) Kwale Sub-humid 3 66.7 33.3 0.0 Tharaka-Nithi Humid 72 90.3 87.5 79.2 Kisii Temperate 46 73.9 71.7 67.4 Bungoma Temperate 23 47.8 34.8 34.8 All 144 77.8 72.9 66.7 County Prev. A. mean Median G. mean >20ppb (ppb) (ppb) (ppb) (%) Kwale 0.0 3.5 4.2 2.3 Tharaka-Nithi 48.6 348.3 19.4 24.7 Kisii 56.5 61.0 26.3 13.9 Bungoma 21.7 16.8 0.4 2,6 All 45.8 196.4 17.2 13.7 Prev.: prevalence; A. mean: arithmetic mean; G. mean: geometric mean; one part per billion (ppb) is the limit of detection Table 6: Prevalence and levels of aflatoxin B1 in farmers feeds during dry and rainy seasons in Kenya County AEZ Samples Range A. mean G. mean (Season) (ppb) (ppb) (ppb) Kwale Sub-humid 1 (dry) 0.8 N/A N/A Tharaka-Nithi Humid 20 (dry) <1-28.5 13.2 8.6 Kisii Temperate 30 (dry) <1-68 19.9 5.2 Bungoma Temperate 11 (dry) <1-85 22.2 3.8 County Samples Range A. mean G. mean p (Season) (ppb) (ppb) (ppb) Kwale 2 (rainy) 4.2-5.9 4.9 4.8 0.31 Tharaka-Nithi 52 (rainy) <1-9661 477.3 37.1 0.02 Kisii 16 (rainy) 12-345 138.1 88.7 0.03 Bungoma 12 (rainy) <1-81 12.0 1.9 0.46 AEZ: agro/ecological zone; A. mean: arithmetic mean; G. mean: geometric mean; p: two/sample Wilcoxon sign/rank test at 95% level of confidence; one part per billion (ppb) is the limit of detection; N/ A: not applicable Table 7: Prevalence and levels of aflatoxin Ml in milk from farmers in Kenya County AEZ N Prev. Prev. Prev. >2ppt (%) >5ppt (%) >20ppt (%) Kwale Sub-humid 59 13.6 11.9 3.4 Isiolo Semi-arid 110 37.3 27.3 9.1 Tharaka-Nithi Humid 126 65.1 50.8 36.5 Kisii Temperate 111 31.5 20.7 16.2 Bungoma Temperate 106 34.9 31.1 17.0 All 512 39.7 30.7 18.4 County Prev. Prev. Range A. mean Median >50ppt (%) >100ppt (%) (ppt) (ppt) (ppt) Kwale 3.4 3.4 <2-486 13.7 0 Isiolo 3.6 0.9 <2-820 14.1 0 Tharaka-Nithi 26.2 10.3 <2-6999 98.7 5.1 Kisii 7.2 4.5 <2-465 16.2 0 Bungoma 5.7 1.9 <2-230 12.0 0 All 10.4 4.5 <2-6999 34.9 0 County G. mean (ppt) Kwale 1.5 Isiolo 2.5 Tharaka-Nithi 8.4 Kisii 2.4 Bungoma 2.8 All 3.2 AEZ: agro-ecological zone; N: number of milk samples; Prev.: prevalence mean: arithmetic mean; G. mean: geometric mean; two parts per trillion (ppt) is the limit of detection Table 8: Prevalence of aflatoxin Ml in milk during dry and rainy seasons in the study sites in Kenya County AEZ N (season) Range A. mean G. mean (ppt) (ppt) (ppt) Kwale Sub-humid 30 (dry) <2-256 10.7 1.9 Isiolo Semi-arid 56 (dry) <2-70 3.6 1.7 Tharaka-Nithi Humid 64 (dry) <2-359 32.3 7.9 Kisii Temperate 63 (dry) <2-216 13.2 2.4 Bungoma Temperate 64 (dry) <2-230 16.2 4.0 County N (season) Range A. mean G. mean p (ppt) (ppt) (ppt) Kwale 29 (rainy) <2-486 16.7 1.2 0.02 Isiolo 54 (dry) <2-820 24.9 3.8 0.02 Tharaka-Nithi 62 (rainy) <2-6999 167.6 9.0 0.75 Kisii 48 (rainy) <2-465 20.0 2.4 0.22 Bungoma 42 (rainy) <2-86 5.4 1.6 <0.001 AEZ: agro-ecological zone; N: number of milk samples; A. mean: arithmetic mean; G. mean: geometric mean; p= two-sample Wilcoxon sign-rank test at 95% confidence; two parts per trillion (ppt) is the limit of detection
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|Author:||Senerwa D.M.; Sirma, A.J.; Mtimet, N.; Kangethe, E.K.; Grace, D.; Lindahl, J.F.|
|Publication:||African Journal of Food, Agriculture, Nutrition and Development|
|Date:||Jul 1, 2016|
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