Polythene and plastic-degrading microbes in an Indian mangrove soil.
La biodegradacion de las bolsas de polietileno y vasos de plastico fue analizada despues de 2, 4, 6 y 9 meses de incubacion en suelo de manglar. La biodegradacion de las bolsas fue significativamente mas alta (hasta 4.21% en 9 meses) que los vasos plasticos (hasta 0.25% en 9 meses). Los conteos microbianos en los materiales degradados mostraron hasta 79.67 x [10.sup.4] por gramo para las bacterias heterotroficas totales, y hasta 55.33 x [10.sup.2] por gramo para los hongos. Se identifico 5 especies microbianas Gram positivas, 2 Gram negativas, y 8 especies de hongos del genero Aspergillus en asociaci6n con materiales degradados. Las especies predominantes fueron Streptococcus, Staphylococeus, Micrococcus (Gram +), Moraxella, and Pseudomonas (Grato -) y dos especies de hongos (Aspergillus glacus and A. niger). La eficiencia de las especies microbianas en la degradacion fue analizada en cultivos bajo agitacion. Entre las bacterias, las Pseudomo. nas degradaron 20.54% de polietileno y 8.16% de plasticos en un pertodo de un mes. Entre los hongos, Aspergillus glaucus degrado 28.80% de polietileno y 7.26% de plasticos por mes. Este trabajo revela que el suelo manglar es una buena fuete de microbios capaces de degradar polietileno y plasticos.
Abstract: Biodegradation of polythene bags and plastic cups was analyzed after 2, 4, 6, and 9 months of incubation in the mangrove soil. The biodegradation of polythene bags was significantly higher (up to 4.21% in 9 months) than that of plastic cups (up to 0.25% in 9 months). Microbial counts in the degrading materials were recorded up to 79.67 x [10.sup.4] per gram for total heterotrophic bacteria, and up to 55.33 x [10.sup.2] per gram for fungi. The microbial species found associated with the degrading materials were identified as five Gram positive and two Gram negative bacteria, and eight fungal species of Aspergillus. The species that were predominant were Streptococcus, Staphylococcus, Micrococcus (Gram +ve), Moraxella, and Pseudomonas (Gram-ve) and two species of fungi (Aspergillus glaucus and A. niger). Efficacy of the microbial species in degradation of plastics and polythene was analyzed in shaker cultures. Among the bacteria, Pseudomonas species degraded 20.54% of polythene and 8.16% of plastics in one-month period. Among the fungal species, Aspergillus glaucus degraded 28.80% of polythene and 7.26% of plastics in one-month period. This work reveals that the mangrove soil is a good source of microbes capable of degrading polythene and plastics.
Key words: Rhizophora, Avicennia, plastics, polythene, degradation.
During the past 3-decades, plastic materials have been increasingly used in food clothing, shelter, transportation, construction, medical, and recreation industries. Plastics are advantageous as they are strong, light-weighted, and durable. However, they are disadvantageous as they are resistant to biodegradation, leading to pollution, harmful to the natural environment. The successful production and marketing of biodegradable plastics will help alleviate the problem of environmental pollution. In the past 10 years, several biodegradable plastics have been introduced into the market. However, none of them is efficiently biodegradable in landfills. For this reason, none of the products has gained widespread use (Anonymous 1999). Hence, there is an urgent need to develop efficient microorganisms and their products to solve this global issue.
The polythene is the most commonly found non-degradable solid waste that has been recently recognized as a major threat to marine life. The polythene could sometimes cause blockage in intestine of fish, birds and marine mammals (Spear et al. 1995, Secchi and Zarzur 1999). Degradation of polythene is a great challenge as the materials are increasingly used. A very general estimate of world wide plastic waste generation is annually about 57 million tons (Bollag et al. 2000). This solid waste related problems pose threat to megacities especially coastal ones. The coastal mangroves have historically been favoured dumping sites for the solid waste disposal (Kathiresan and Bingham 2001). An attempt in this paper has been made to isolate potent microorganisms that degrade the plastic materials from the mangrove sediment.
Biodegradation under field conditions: Plastic cups and polythene bags were buried at a depth of 5 cm in the mangrove soil under two zones, colonized by Rhizophora sp. and or Avicennia sp., along the Vellar estuary (11[grados]29' N; 79[grados]46' E; southeast coast of India). The materials were allowed to degrade naturally in the mangrove soil, and they were sampled at the intervals of 2, 4, 6 and 9 months using sterile forceps and transferred to laboratory aseptically. One set of samples was thoroughly washed using distilled water, shade-dried and then weighed for final weight. The degradation was determined in terms of per cent of weight loss of the materials over a period. Another set of sampled materials was washed gently using sterile water to remove soil debris. About one gram of the materials infested with bacteria and fungi was transferred into the conical flask having 99 mi of sterile water. This content, which was shaken vigorously for its equal distribution, was serially diluted. The pour plate method was adopted using the Zobell's agar medium for bacteria and the Martin Rose Bengal medium for fungi. For each dilution, three replicates were made. The plates were then incubated at 30[grados]C for 2-7 days. The bacterial and fungal counts were then made.
Identification of microorganisms: Among the bacterial and fungal colonies, the dominant ones were isolated and sub-cultured repeatedly for getting pure colonies and then preserved in slant tubes for further identification. The bacterial strains were identified based on the keys detailed by Oliver (1982). The tests conducted were motility test, glucose oxidation, penicillin sensitivity, and glucose fermentation for gram-negative bacteria; and, shape, dextrose fermentation, catalase and glucose utilization for gram-positive bacteria. The fungal strains were identified after staining them with cotton blue, by following the keys of Raper and Fennell (1987).
Microbial degradation of plastics under laboratory conditions: To assess this, the preweighed discs of 1-cm diameter prepared from polythene bags and disposable plastic cups were aseptically transferred to the conical flask containing 50 ml of culture broth medium, inoculated with different bacterial and fungal species separately. Nutrient broth medium was used for bacteria and Rose Bengal broth medium for fungi. Control was maintained with plastic discs in the microbe-free medium. Four flasks were maintained for each treatment and left in a shaker. After one month of shaking, the plastic discs were collected, washed thoroughly using distilled water, shade-dried and then weighed for final weight. From the data collected, weight loss of the plastics and polythene bags, was calculated.
In situ degradation of plastics in the mangrove soil: An experiment was designed for degrading the polythene bags and plastic cups, under the soil conditions of two mangrove species. The results are shown in Table 1. Irrespective of mangrove zone, the polythene bags were found degraded after 6 and 9 months, but not after 2 and 4 months of incubation in the soil. The plastic cups were found degraded only after 9 months, but not after 2,4 and 6 months of analysis. The biodegradation of polythene was maximum of 3.77% and 4.21% respectively under Rhizophora and Avicennia zones, after 9 months of analysis, and the corresponding values for the biodegradation of plastics were only 0.25% and 0.17% (Table 1).
Microbial counts in degrading plastics: The microbial counts on the polythene bags and plastic cups that degraded for different months under two mangrove zones were recorded. However, there was no statistically significant variation of bacterial counts between the two mangrove zones (Rhizophora and Avicennia) and or between the months of degradation (Data not shown in table).
The counts of total heterotrophic bacteria (THB) ranged from 24.50 x [10.sup.4] (2-month) to 64.83 x [10.sup.4] (6-month) in the polythene degraded under Avicennia soil, and from 41.33 x [10.sup.4] to 64.83 x [10.sup.4] under Rhizophora soil. The THB counts ranged from 67.33 x [10.sup.4] (2-month) to 79.67 x [10.sup.4] (6-month) in the plastic degraded under Rhizophora soil, and from 43.17 x [10.sup.4] to 52.83 x [10.sup.4] under Avicennia soil.
The fungal counts ranged from 13.50 x [10.sup.2] (2-month) to 55.33 x [10.sup.2] (6-month) in the polythcne degraded under Rhizophora soil, and from 44.73 x [10.sup.2] to 62.67 x [10.sup.2] under Avicennia soil. The fungal counts varied from 35.17 x [10.sup.2] (2 month) to 50 x [10.sup.2] (6-month) for the plastics degraded under Avicennia soil, and from 24.17 x [10.sup.2] to 47.33 x [10.sup.2] under Rhizophora soil.
Microbes identified: The microbial species identified from degrading polythene bags were Bacillus sp., Staphylococcus sp., Streptococcus sp., Diplococcus sp., and Micrococcus sp. (belong to Gram- positive bacteria); Moraxella sp. and Pseudomonas sp. (belong to Gram-negative bacteria); and, Aspergillus niger, A. ornatus, A. cremeus, A. flavus, A. candidus, A. ochraceus, A. nidulans, and A. glaucus (belonging to fungi). Thus seven bacterial species and eight fungal species were obtained. These microbial species were also recorded from degrading plastic bags, except Bacillus sp., Diplococcus sp., Aspergillus ornatus, A. cremeus, A. flavus, A. candidus, A. ochraceus, A. nidulans. There were five bacterial and two fungal species, commonly and predominantly found detected in both polythene and plastics, and these were selected for further study.
Microbial degradation of plastics and polythene bags in laboratory: Seven microbial species were tested in the laboratory for their ability of degrading the polythene and plastics. The species tested were Moraxella species, Pseudomonas, Staphyloccoccus, Micrococcus and Streptococcus species and two fungal species--Aspergillus niger and A. glaucus. These microbes were separately allowed to degrade the polythene and plastics under shaker cultures for a month. The results are shown in Table 2.
The bacteria caused the biodegradation ranging from 2.19 to 20.54% for polythene and from 0.56 to 8.16% for plastics. Among the bacteria, Pseudomonas and Moraxella sp. were found most active in degrading 20.54% of polythene, and 8.16 % of plastics in one-month period (Table 2).
Among the species, Aspergillus glaucus was more active than A. niger in degrading 28.8% of polythene and 7.26% of plastics within a month (Table 2).
To our knowledge, there is no report on polythene and plastic degradation in the mangrove environment, which serves as a dumping site of those materials. The biodegradation of the polythene is relatively faster and earlier than that of the plastics. The biodegradation is up to 1.98% after 6 months of analysis for polythene and is up to 0.25% only after 9 months for plastics (Table 1). This may be attributed to the thickness of the polythene that is 5-times thinner than the plastics.
The plastic materials have been degraded in the mangrove soil irrespective of the mangrove zones. This reveals that the mangrove soil can be a source of factors responsible for the degradation of plastic materials. The factors may include microbes besides moisture, heat etc. (Anonymous 1999). The mangrove soil maintains moisture by tidal water flood during high tide and the soil gets heated during low tide when exposed to sunlight as well due to exothermic reactions of biological compounds in the soil (Kathiresan and Bingham 2001). Besides these abiotic conditions, microbial counts are also high, perhaps favouring the degradation of plastics. For example, the plastic materials in mangrove soil have shown rich total heterotrophic bacterial counts of up to 79.67 x 104 and fungal counts of up to 55.33 x 102, and the plastic materials have been colonized commonly by five species of bacteria and two species of fungi.
It has experimentally been proved that these microbes cause degradation of plastic materials up to 28.8% within a month. Among these microbes, the strains ofAspergillus glautus, A. niger, Pseudomonas sp. and Moraxella sp. are efficient in biodegradation (Table 2). The mechanism of degradation is not known. The surface of plastic materials has turned from smooth to rough with cracking. This may be due to the compounds secreted extracellularly by the microbes that may break the complex molecular structure of plastics. Hence, further study on microbial enzymes or organic acids in degradation of the polythene and plastics will pave way for finding technology for degrading the plastic materials, which are otherwise hazardous to environment.
TABLE 1 Biodegradation of polythene bags and plastic cups buried for different duration under two mangrove zones along the Vellar estuary Month of analysis Biodegradation (% weight loss) Rhizophora zone Polythene Plastic 2 0 0 4 0 0 6 1.98 [+ o -] 0.29 0 9 3.77 [+ o -] 0.29 0.17 [+ o -] 0.02 Month of analysis Biodegradation (% weight loss) Avicennia zone Polythene Plastic 2 0 0 4 0 0 6 1.74 [+ o -] 0.12 0 9 4.21 [+ o -] 0.31 0.25 [+ o -] 0.03 Values between months of analysis are significant at 5%, but non-significant between mangrove zones. TABLE 2 Degradation of the polythene and plastics incubated with different microbial species in shaker cultures under laboratory conditions Name of microbe Microbial degradation (% weight loss / month) Polythene Plastics Bacteria: Pseudomonas sp. 20.54 [+ o -] 0.13 3.97 [+ o -] 0.21 Staphyloccoccus sp. 16.39 [+ o -] 0.01 0.56 [+ o -] 0.04 Moraxella sp. 7.75 [+ o -] 0.61 8.16 [+ o -] 0.65 Micrococcus sp. 6.61 [+ o -] 0.42 1.02 [+ o -] 0.08 Streptococcus sp. 2.19 [+ o -] 0.15 1.07 [+ o -] 0.05 Fungi: Aspergillus glaucus 28.80 [+ o -] 2.40 7.26 [+ o -] 0.51 Aspergillus niger 17.35 [+ o -] 2.00 5.54 [+ o -] 0.32 Significant at 5% between species and between plastics and polythene.
The author is thankful to the Director and authorities of Annamalai University for providing facilities and Dr. N. Rajendran for his help.
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Centre of Advanced Study in Marine Biology, Annmnalai University, Parangipettai 608 502, India. Phone: +91 4144 243533 Ext. 210 (Office) +914144 238419 (Home). Fax: +91 4144 238419 / 243555; email@example.com; firstname.lastname@example.org
Received 13-V-2002. Corrected 27-VI-2003. Accepted 30-VI-2003.
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|Publication:||Revista de Biologia Tropical|
|Date:||Sep 1, 2003|
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