Printer Friendly

Polysomy 8 Syndrome: A Distinct Clinical Entity Comprising of Myelomonocytic/Monocytic Lineage Involvement in Acute Leukemia.


Cytogenetic analysis of bone marrow cells reveals non-random chromosomal aberrations associated with distinct subsets of hematological malignancies. Thus karyotypes of patients provide powerful diagnostic/prognostic indicators for leukemia and lymphoma subgroups. The WHO 2008 classification of tumors of the hematopoietic system uses common genetic findings in addition to morphologic, clinical and immunophenotypic features to define distinct diagnoses (1). However, there is a lack of consensus as to the diagnostic and prognostic significance of rare non-random aberrations. Considering that cytogenetics provides the framework for the development of risk stratified therapeutic strategies (2), it is extremely important that recurrently reported, less frequent karyotypes with their associated clinical and cellular phenotypical/morphological features should be recognized to establish better standardized treatment approaches and patient management. Polysomy 8 is one of such rare karyotypic abnormalities that define a distinct biological subgroup of myeloid hematopathologies. It has been associated with myelomonocytic/monocytic lineage involvement, poor prognosis and high incidence of myeloid sarcoma. We describe a patient diagnosed as AML-M5, with myeloid sarcoma and tetrasomy 8 as the sole chromosomal abnormality.


59 years old male patient was admitted to an outpatient clinic with complaints of progressive fatigue and dyspnea on exertion. Blood count revealed pancytopenia and the patient was referred to our hematology department. At admission the differential blood count was as follows: Hb: 7 g/dl, Hct: 20.9%, WBC: 1900/[micro]l, Neu: 700/[micro]l, Ly: 1100/[micro]l, Mo: 100/[micro]l, Platelet: 81000/[micro]l. Past medical history revealed a basal cell carcinoma at the left frontal region of the head which was radiated after excision, two years before admission. No other ongoing medication or chronic illness was recorded. Upon physical examination, neither lymphadenopathy nor organomegaly was detected. There was a guttate, hyperemic and squamated purple papular lesion, approximately 1 cm in diameter on the left frontal region of the forehead. Peripheral blood smear revealed 80% monoblasts. Bone marrow aspiration revealed an 80-90% blastic infiltration with suppressed erythropoiesis and megakaryopoiesis. Based on blast morphology, FAB M5 AML was diagnosed. Flow cytometric analysis of the blastic population was also consistent with a pure monoblastic cell population. The pathological evaluation of the skin lesion revealed a myeloid sarcoma. The patient was put on 7 + 3 induction regimen which included cytarabine (200 mg/[m.sup.2] x 2/7 days) and idarubicin (12 mg/[m.sup.2]/day). After the first cycle of induction regimen, complete remission was achieved. But disease was relapsed after the second cycle of induction therapy. Allergenic bone marrow transplantation is recommended.



Cytogenetic analysis was performed on bone marrow samples directly after aspiration and following 24 h short term culture (McCoy's 5A culture medium, 10% FBS) without mitotic stimulation. Harvesting of the cells and slide preparation were done according to standard methods. Chromosomes were banded with GTG technique. Twenty metaphase spreads were analyzed and karyotyped in accordance with ISCN 2013 (3).

Molecular Genetics

Fluorescence in situ hybridization (FISH) analyses were performed at complete remission stage on interphase nuclei from bone marrow culture as described above. The AML1/ETO translocation, dual fusion (Cytocell Ltd, Cambridge, UK), the chromosome 8 satellite enumeration (C-MYC/SE 8, KREATECH Diagnostics, The Netherlands), and the MLL break apart (Kreatech, Amsterdam, Netherlands) probes were used. At least 200 cells were analyzed for each probe cocktail.


All metaphases examined on the bone marrow sample obtained prior to treatment revealed tetrasomy 8 as the sole karyotipic change (Fig. 1). No metaphase with trisomy 8 was observed. Post-treatment bone marrow cytogenetic analysis during remission displayed normal karyotype.

FISH analysis with AML1/ETO revealed tetrasomic signals for ETO (8q21) in 5.5% of the cells analyzed (Fig. 2). The presence of tetrasomy was confirmed with C-MYC (8q24) and chromosome 8 centromeric probe cocktail. FISH analysis with MLL (11q23) break apart probe ruled out a cryptic MLL gene rearrangement (intact signals were observed).


Although trisomy 8 is one the most common chromosomal abnormalities observed in hematological malignancies (4), polysomy 8 is a rare, non-random numerical abnormality associated with myeloid malignancies such as acute myelod leukemia (AML), myelodysplastic syndromes (MDS) and rarely myeloproliferative disorders (MPDs) (4, 5). Tetrasomy 8 is the most common of the polysomies, first described in AML in 1987 (6). Since then it has been reported in 129 cases comprising of AML, MDS and MPDs as a sole anomaly or part of a complex karyotype. To date there are only two case where tetrasomy 8 was reported in patients with lymphoid lineage pathology (7, 8). Aside from these two cases, common characteristics of patients-irrespective of their clinical presentation-are: a predominant myelomonocytic/monocytic lineage involvement; poor prognosis (median survival 7 months) and a high incidence of extramedullary involvement (myeloid sarcoma), mainly as skin lesions. In 2005 Beyer et al. reported a slight male gender predominance; in addition to MLL gene rearrangements described in 19 of the 103 published cases (16%) (9). Our literature search provided 11 new patients with MLL gene rearrangements, bringing the total to 30 cases out of 129 (23.2%) (9, 10-14). The high occurrence of MLL rearrangements possibly contributes to the adverse prognosis observed in patients with this genotype.

In most cases in which polysomy 8 was reported as the only karyotypic abnormality, cryptic MLL rearrangements-when investigated-were found to be rare (9). Our results are in line with the previously published cases in this respect.

Trisomy 8 was undetectable at diagnosis with conventional cytogenetics in 75% of all reported cases. In nearly all cases that were further examined with FISH, a concurrent trisomy 8 clone was also detected. It has been suggested that tetrasomy 8 occurred subsequent to trisomy 8 by serial clonal evolution from a normal karyotype to tetrasomy 8 (8, 13, 15-18). An alternative explanation would be the segregation lag of all four chromatids during mitosis and trisomy 8 resulting from the subsequent loss of one of the four chromosome 8 s'. In the patient presented here, conventional cytogenetics was performed at presentation and revealed tetrasomy 8 as a sole anomaly in all metaphases examined. FISH analysis was carried out on a post-treatment sample and tetrasomy 8 was detected in approximately 6% of the interphase nuclei; no trisomy 8 clones were observed.

The observation that the trisomy 8 clone is usually not detected by conventional cytogenetics has been proposed to be a result of the high proliferative capacity of the tetrasomy clone (19). This proliferative advantage of the tetrasomy clone is one of the features that is thought to contribute to the aggressive phenotype and poor prognosis. The involvement of the chromosome 8 in myeloid leukemias suggests that dosage sensitive, cell type specific genes are localized on chromosome 8. These genes located on chromosome 8-such as c-MYC in 8q24, MOS in 8q22, ETO in 8q21.3-may play a significant role in the biology of myeloid lineage disorders via increased gene dosage leading to over-expression (14). Interestingly the aggressive phenotype and poor prognosis associated with tetrasomy 8 is not limited to hematological malignancies. There have been two cases of Ewing sarcoma, where an in vitro proliferative advantage and aggressive behavior of the tetrasomy 8 clone has been reported (20, 21).

Numerical aberrations of chromosome 8 from trisomies to polysomies, have been describes in nearly all myeloid lineage disorders, corresponding predominantly to neoplasms with monocytic differentiation (17, 22, 23). Myelomonocytic/monocytic lineage involvement is also a characteristic of polysomy 8 cases. It is tempting to speculate that in the absence of signals required for differentiation of the granulocytic lineages, the monocytic lineage is the default route of differentiation for the myeloblast. Thus, the gain of extra chromosome 8's may cause a block in the differentiation route of granulocytes, skewing cells towards a default myelomonocytic pathway.

Our patient was presented with myeloid sarcoma, another feature observed at a high incidence in patients with polysomy 8 (13, 23, 24). It is not clear how tetrasomy 8 can predispose to the development of an extramedullary disease. One explanation may be the gain or over-expression of genes such as FAK, FGFR1, EXT1, ASAP1, ADAM9, HPT1, RHOBTB2, and SYCL associated with cell adhesion, cell-cell contacts, migration and extracellular matrix interactions.

In conclusion, we believe there are significant number of cases reported to date to support the presence of a distinct clinical entity: polysomy 8 syndrome (25). The poor prognosis associated with this genotype calls for reconsidering therapeutic options such as considering these patients for allogenic bone marrow transplantation immediately after first remission. Cytogenetic abnormalities have been proven to be among the most valuable prognostic indicators in leukemia, allowing the stratification of patients in risk groups associated with different clinical outcomes. Recognition of the polysomy 8 syndrome will allow for the development of a standardized approach to these patients; as well as stimulating further research into the biology of the disorder that will allow for the development of better therapeutic strategies.

Ethics Committee Approval: All the work herein has been done as part of routine patient care and diagnostics. A separate ethics clearance was not taken.

Informed Consent: Informed consent was obtained from all individual participants included in the study

Peer-review: Externally peer-reviewed.

Author Contributions: Concept - ZY, EY; Design - ZY, EY; Supervision - ZY; Resource - ZY, OGS, IA, OA; Materials -IA,OGS; Data Collection and/ or Processing - ZY, EY, MP, OGS, IA, OA; Analysis and/or Interpretation - ZY, EY, MP; Literature Search- ZY, EY, MP; Writing - ZY, EY; Critical Reviews - OA.

Conflict of Interest: No conflict of interest was declared by the authors.

Financial Disclosure: The authors declared that this study has received no financial support.


(1.) Vardiman JW, Thiele J, Arber DA, et al. The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes. Blood 2009;114:937-951. [CrossRef]

(2.) Grimwade D, Hills RK, Moorman AV, et al.; National Cancer Research Institute Adult Leukaemia Working Group. Refinement of cytogenetic classification in acute myeloid leukemia: determination of prognostic significance of rare recurring chromosomal abnormalities among 5876 younger adult patients treated in the United Kingdom Medical Research Council trials. Blood 2010;116:354-365. [CrossRef]

(3.) Shaffer LG, Mc Gowan-Jordan J, Schmid M. ISCN 2013: an international system for human cytogenetic nomenclature. Basel: S. Karger; 2013.

(4.) Kim J, Park TS, Song J, Lee KA, Lee SG, Cheong JW, Choi JR. Tetrasomy 8 in a patient with acute monoblastic leukemia. Korean J Lab Med 2008;28:262-266. [CrossRef]

(5.) Aktas D, Tuncbilek E, Cetin M, Hicsonmez G. Tetrasomy 8 as a primary chromosomal abnormality in a child with acute megakaryoblastic leukemia: a case report and review of the literature. Cancer Genet Cytogenet 2001;126:166-168. [CrossRef]

(6.) Sheila N, Sait J, Raza A, Sandberg. Tetrasomy of chromosome 8: An interesting and rare cytogenetic phenomenon in acute nonlymphocytic leukemia. Cancer Genet Cytogenet 1987;27:269-271. [CrossRef]

(7.) Shao J, Zhang L, Semenza JC, Beach B, Smith MT. Tetrasomy 8 detected by interphase cytogenetics in a child with acute lymphocytic leukemia. Cancer Genet Cytogenet 1996;92:135-140. [CrossRef]

(8.) de Oliveira FM, Brandao RA, Leite-Cueva SD, et al. Tetrasomy 8 in a patient with chronic lymphocytic leukemia. Cancer Genet Cytogenet 2010;198:166-169. [CrossRef]

(9.) Beyer V, Muhlematter D, Parlier V, et al. Polysomy 8 defines a clinicocytogenetic entity representing a subset of myeloid hematologic malignancies associated with a poor prognosis: report on a cohort of 12 patients and review of 105 published cases. Cancer Genet Cytogenet 2005;160:97-119. [CrossRef]

(10.) Shin SY, Koo SH, Kwon KC, et al. Chromosome 8 pentasomy with partial tandem duplication of 11q23 in a case of de novo acute myeloid leukemia. Cancer Genet Cytogenet 2009;194:44-47. [CrossRef]

(11.) Kameoka J, Horiuchi T, Miyamura K, et al. Acute monoblastic leukemia with tetrasomy 8. Rinsho Ketsueki 2006;47:770-776.

(12.) Makis W, Rakheja R, Lavoie J, Hickeson M. Myeloid Sarcoma and Acute Myelomonocytic Leukemia in an Adolescent with Tetrasomy 8: Staging with (18)F-FDG PET/CT. Nucl Med Mol Imaging 2012;46:119-124. [CrossRef]

(13.) Takahashi T, Tsukuda H, Kimura H, Yoshimoto M, Tsujisaki M. Extramedullary relapse of AML with t(9;11)(p22;q23) associated with clonal evolution from trisomy 8 into tetrasomy 8. Intern Med 2010;49:447-451. [CrossRef]

(14.) Tsirigotis P, Papageorgiou S, Abatzis D, et al. Acute myelogenous leukemia with tetrasomy 8 is a disease with a poor prognosis. Cancer Genet Cytogenet 2005;161:78-81. [CrossRef]

(15.) Jani Sait SN, Raza A, Sandberg AA. Tetrasomy of chromosome 8: an interesting and rare cytogenetic phenomenon in acute nonlymphocytic leukemia. Cancer Genet Cytogenet 1987;27:269-271. [CrossRef]

(16.) Sole F, de Pablos JM, Woessner S, et al Coexistence of tetrasomy 8 and trisomy 8 in a case with myeloid metaplasia with myelofibrosis. Cancer Genet Cytogenet 1997;94:147-150. [CrossRef]

(17.) Ferro MT, Vazquez-Mazariego Y, Ramiro S, et al. Trisomy/ tetrasomy of chromosome 8 and +i(8q) as the sole chromosome abnormality in three adult patients with myelomonocytic leukemia. Cancer Genet Cytogenet 2000;120:163-165. [CrossRef]

(18.) Kameoka J, Funato T, Obara Y, et al. Clonal evolution from trisomy into tetrasomy of chromosome 8 associated with the development of acute myeloid leukemia from myelodysplastic syndrome. Cancer Genet Cytogenet 2001;124:159-164. [CrossRef]

(19.) Yan J, Marceau D, Drouin R. Tetrasomy 8 is associated with a major cellular proliferative advantage and a poor prognosis. two cases of myeloid hematologic disorders and review of the literature. Cancer Genet Cytogenet 2001;125:14-20. [CrossRef]

(20.) Abdelmoula NB, Landman-Parker J, Tourniaire B, et al. An aggressive Ewing sarcoma associated with a new variant translocation, t(4;11;22) (q25;q24;q12), hyperdiploid karyotype, and tetrasomy 8. Cancer Genet Cytogenet 2005;163:186-188. [CrossRef]

(21.) Trakhtenbrot L, Neumann Y, Mandel M, et al. In vitro proliferative advantage of bone marrow cells with tetrasomy 8 in Ewing sarcoma. Cancer Genet Cytogenet 1996;90:176-178. [CrossRef]

(22.) Lessard M, Herry A, Berthou C, et al. Chromosome 8 tetrasomies and pentasomies--a clonal abnormality closely associated with acute monocytic leukaemia. Leuk Lymphoma 1997;27:127-135. [CrossRef]

(23.) Zhang XX, Robinson LJ, Stenzel TT, Qumsiyeh MB. Translocation (15;17)(q22;q21) as a secondary chromosomal abnormality in a case of acute monoblastic leukemia with tetrasomy 8. Cancer Genet Cytogenet 1999;113:9-13. [CrossRef]

(24.) Alonso-Dominguez JM, Calbacho M, Talavera M, et al. Cytogenetics findings in a histiocytic sarcoma case. Case Rep Hematol 2012;2012:428279. [CrossRef]

(25.) Mitelman Database of Chromosome Aberrations and Gene Fusions in Cancer, 2014. Mitelman F, Johansson B, Mertens F, editors. [CrossRef]

Zeynep Yuce (1), Erdinc Yuksel (2), Melek Pehlivan (3), Omur Gokmen Sevindik (4), Inci Alacacioglu (4), Oguz Altungoz (1)

(1) Dokuz Eylul University Faculty of Medicine, Department of Medical Biology, Izmir, Turkey

(2) Dokuz Eylul University Hospital, Central Diagnostic Laboratory, Izmir, Turkey

(3) Izmir Katip Celebi University, Vocational School of Health Services, Izmir, Turkey

(4) Dokuz Eylul University, Faculty of Medicine, Department of Hematology, Izmir, Turkey

Address for Correspondence: Zeynep Yuce, E-mail:

Received: 21.10.2018; Accepted: 21.02.2019; Available Online Date: 28.05.2019

Cite this article as: Yuce Z, Yuksel E, Pehlivan M, Sevindik OG, Alacacioglu I, Altungoz O. Polysomy 8 Syndrome: A Distinct Clinical Entity Comprising of Myelomonocytic/Monocytic Lineage Involvement in Acute Leukemia J Basic Clin Health Sci 2019; 3:42-45.
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2019 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Original investigation
Author:Yuce, Zeynep; Yuksel, Erdinc; Pehlivan, Melek; Sevindik, Omur Gokmen; Alacacioglu, Oguz
Publication:Journal of Basic and Clinical Health Sciences
Date:May 1, 2019
Previous Article:Time Estimation and Risk Taking Behavior in Type A Personality.
Next Article:The Notch Types of the Glenoid Cavity in Adult Dry Human Scapulae.

Terms of use | Privacy policy | Copyright © 2020 Farlex, Inc. | Feedback | For webmasters