Physical and chemical variables influencing riverine biota in a tropical river of northern Mindanao Philippines.
In a riverine system, the physico-chemical variables, in addition to hydrological conditions are the pivotal factors for macroinvertebrate and microalgae communities. The abundance of these organisms is determined by the resource availability, biotic interactions, and prevailing water quality. Water quality parameters play a major role in aquatic ecosystem health. Any change in water quality can lead to variations in compositions of plants and animal species. The most important water quality parameters that impact on aquatic ecosystems include temperature, salinity  acidity, Total Dissolved Solids (TDS), Dissolved oxygen (DO), Biological Oxygen Demand (BOD), pH  and nutrients . Nutrient input into the stream due to agricultural land use and removal of riparian vegetation led to degraded water quality and increased conductivity. Differentiation of the physico-chemical parameters in the stream between agricultural and forest areas affected the benthic macroinvertebrates  and the microalgae .
The relationship between biotic indices and water quality parameters proved to be a useful tool for water resource managers to assess the ecosystem status when only physicochemical properties of water are known [6, 7]. The use of riverine biota is based on the assumption that disturbances alter biotic communities, changing their density or causing functional changes in the ecosystem . Findings of many studies on river habitats suggested that specific physical and chemical variables influence the distribution of organisms . Families of macroinvertebrates vary in their sensitivity to disturbances such as pollution . Moreover, their relative abundance is used to infer the severity of contamination [9, 11]. The species of algae that develop in an area depend on different environmental factors  making them good indicators of water quality . Among the algae, diatoms have the advantage of being easy to collect and store due to their hard frustules. They also respond rapidly to changes in environmental variables such as light, pH, temperature and nutrients .
This study explored the assemblage of macroinvertebrates and benthic microalgae in a tropical river as influenced by environmental variables. These organisms are sensitive to changes in physical and chemical conditions of river systems. Certain physical and chemical conditions that existed at the time of field sampling were measured to make inferences on the influence of these conditions to the focused organisms. Our research incorporated both biotic and abiotic components of the lotic ecosystem of Cagayan de Oro River, a major river system of Cagayan de Oro City, Mindanao, Philippines. The river has its headwaters and tributaries in the central part of the province of Bukidnon. It flows northward towards Cagayan de Oro City traversing three municipalities at the same time picking up tributaries along the way. The river empties into Macajalar Bay of Misamis Oriental, stretching about 90 kilometers. The river has a catchment area of approximately 152,000 hectares, eighty percent is located in the province of Bukidnon, and the rest is in the province of Lanao del Norte and Cagayan de Oro City. The river serves as a boundary between the provinces of Bukidnon and Lanao del Norte, and between Bukidnon and Cagayan de Oro City. The river basin is of economic, social, and leisure activities. It is the major source of water for domestic, industrial, agricultural, and hydroelectric power generation of a progressing urban community.
MATERIALS AND METHODS
Field samplings were done twice in 2010-2011 in seventeen (17) sites, 9 from the main channel and 8 from tributaries of Cagayan de Oro River, Mindanao Philippines (Figure 1). Biotic sampling were in 3 replicates per site at a sampling reach of 150 meters. Water samples for physico-chemical parameters were collected in the middle portion of the river channel where mixing of water mostly occurred. The physical profile of the river including channel width, depth and flow velocity were measured. Determination of elevation and coordinates used Global Positioning System (Magellan brand).
Field sampling of macroinvertebrates used the "kick" net method (mesh size of 0.3 mm) with net "kicks" duration of three minutes per sampling site. The samples collected per net "kicks" were pooled, and obtained three composite field samples from each site. Each field sample was added with equal volume of 95% ethyl alcohol for preservation. In the laboratory, macroinvertebrates were sorted and concentrated into 100 mL A sub-sample of 10 mL per sampling site was counted and identified with the aid of a stereo-binocular dissecting microscope.
Benthic microalgae assemblage were collected by picking ten stones with biofilm (20 cm or greater) from the river channel. The biofilm of the stone's upper surface was designated with a 1.5-inch delimiter which was brushed using a firm-bristled toothbrush. The brushed surfaces per site were rinsed with 50 mL river water and fixed in 10% formalin. A volume of 0.101 mL of sub-sample of microalgae was the basis for the counting and identification. Counting used the nanoplankton counting chamber, and genus identification used the 400x Leica light microscope.
Determination of nutrients such as nitrate, phosphate, nitrite, and silicate used spectrophotometry. The total dissolved solids (TDS) and the hydrogen potential (pH) were measured in situ using Sartorius pH-TDS meter. Temperature, conductivity and dissolved oxygen (DO) was measured in situ using DO-TemperatureConductivity meter (JENWAY). Total suspended solids (TSS) were determined using gravimetric method.
Encoding of data and descriptive statistics were determined using Microsoft EXCEL. The influence of physical and chemical variables on the biota assemblage used the multivariate data analysis software (CANOCO 4.5).
RESULTS AND DISCUSSIONS
A total of one thousand nine hundred sixty two (1,962) individuals of macroinvertebrates were counted and identified. The macroinvertebrate assemblages comprised a total of 63 families, 30 of which were commonly found in both main channel and tributaries. Fifteen (15) families were exclusively observed in the main channel, and 18 families in the tributaries. Many of these families belonged to the orders Ephemeroptera (13 families), Trichoptera (11 families), Coleoptera (8 families) and Diptera (7 families).
Table 1 shows the relative abundance of the macroinvertebrate families. In the main channel, the ephemeropteran Family Baeticidae and the dipteran larvae of Family Chironomidae were almost equally abundant, 22.46% and 22.94%, respectively. These were followed by another two ephemeropterans Family Baetidae (9.78%) and Family Trichorythidae (9.19%). In the tributaries, Family Chironomidae was the most abundant (26.54%) followed by two ephemeropterans of the Family Baeticidae (15.57%) and Family Baetidae (11.02%). Baetids were consistently present in all sampling sites, both in the main channel and in the tributaries. Chironomids were almost always present in all sampling sites. About 48% of the comprising families represented the more sensitive orders Ephemeroptera (mayflies), Plecoptera (stoneflies), and Trichoptera (caddis flies) indicating that less sensitive taxa are more diverse than the more sensitive ones. Among these three orders, Plecoptera were very few and observed only in the upstream tributaries where sedimentation was minimal.
The abundance of a number of insect taxa decreased as the proportion of fine sediments of the river bed increased [15, 6]. The abundance of Baetidae and Caenidae decreased from the headwaters to low lying areas of the river and replaced by Chironomidae. Plecopterans develop only in cold, clear springs and are sensitive to low oxygen concentration and organic pollution . Land use, slope and substratum were the effective variables for the assemblage of macroinvertebrates .
Benthic Microalgae Assemblage:
Benthic microalgae from tributaries and the main channel of the river (Table 2) consisted of thirty (30) genera belonging to five groups namely; Chlorophyceae (6), Euglenophyceae (2), Xanthophyceae (1), Bacillariophyceae (19) and Cyanophyceae (2). The Bacillariophyceae (also known as diatoms) had the highest number of genera. The genera Coscinodiscus, Diadismes, Rhizosolenia, Ankistrodesmus, Cladophora, Tribonema and Spirulina were present only in the tributaries. While density of Navicula was highest in both main channel and tributaries, Oscillatoria was exclusively densest in the main channel as Fragilaria was to the tributaries.
Benthic microalgae stabilize substrata and serve as habitat for many other organisms [19, 20]. These are attached to the substrate, and most likely affected by physical, chemical and biological factors occurring in the stream reach . The abundance of the diatom group in this study, is similar to other works [20, 22, 23, 24, 25]. Diatoms are large and diverse group distributed throughout the world in nearly all types of aquatic systems, and are one of the most important food resources in freshwaters .
Species of the genus Navicula are known to crawl towards the upper surface if they are covered by silt . The high density of Oscillatoria may indicate nitrogen enrichment because blue-green algae have a competitive edge over other algae if bioavailable nitrogenous radicals (nitrate and ammonia) are in short supply. Cocconeis density increases in response to general impairment in streams and rivers as well as to sediment, nutrient and metals impairment while Surirella increase in response to sediment impairment .
Physical and Chemical variables that significantly explain biological assemblage:
There is a wide range of measurements of each physical and chemical variables (Table 3) in every sampling site. The mean values of the variables measured, except for Total Suspended Solids (TSS), were within the acceptable level of Philippine water quality standards. The main channel of the river had high TSS and appeared turbid. The high TSS impacts water quality and deposition of sediments in downstream water bodies and reservoirs. Its high TSS concentration was attributed to accumulation of sediments from eroded tributaries. These water quality evaluation based on physical and chemical methods are only instantaneous measurements , and therefore restraining the knowledge of water conditions only to the moment when the measurements were taken.
The health of the water environment could be assessed most importantly by the abundance, distribution and diversity of the benthic macroinvertebrates and microalgae. At the regional scale, nutrient-rich water bodies can collectively host high species richness . Species of diatoms are characteristics of small streams and benthic invertebrates are associated with larger stream types. The trend was likely the consequence of multiple anthropogenic pressures that have affected lowland rivers . In environmental degradation, biodiversity loss also occurs .
Redundancy Analysis (RDA) of environmental variables with macroinvertebrates showed that the variance in axis 1 was 46.6% and 61.3% in axis 2 (Table 4). These support a highly significant impact of environmental variables to macroinvertebrate assemblage. The first ordination axis significantly showed that pH (r=-0.46) had negative relationship with macroinvertebrates, whereas positive for nitrate (r=0.03). The associations between macroinvertebrates and abiotic conditions can be river basin-specific and are not automatically applicable across river basins in the tropics . For instance, Ephemeroptera, Plecoptera and Trichoptera had very strong negative correlation with pH, BOD, phosphate and TSS but were positively correlated with DO , temperature and pH and between ammonia and nitrates . Benthic macroinvertebrates in general have negative correlation with pH, dissolved oxygen, Biological Oxygen Demand, alkalinity, chloride and phosphate .
RDA of environmental variables and microalgae showed that the variance in axis 1 was 47.6% and 73.3% in axis 2 (Table 4) indicating a highly significant influence on microalgae. The first ordination axis showed pH (r=-0.872), TDS (r=-0.254) and nitrite (r=-0.267) had a significant negative relationship with benthic microalgae while BOD (r=0.453) and temperature (r=0.053) were positively correlated. Other environmental variables in the data set, were total suspended solids (TSS), dissolved oxygen (DO), phosphate, nitrate and silicate did not show significant influence. Studies in urban and rural streams showed that variables such as total nitrogen, electrical conductivity, DO, BOD and velocity have the greatest influence on the assemblage structure of microalgae . In karst springs microalgae assemblage was correlated positively with dissolved oxygen concentration and negatively with specific conductivity and nitrate concentration .
Among the physical and chemical conditions considered in the data set for the ordination analysis, only nitrate and pH significantly described the assemblage of the families of macroinvertebrates (Figure 2). The families preferring higher pH were Perlodidae, Dipseudopsidae and Ephemerellidae (axis 1). While families that increase their abundances with increasing nitrate (axis 2) were Lymnaedae, Planorbidae, Viviparidae, Pilidae and Philopotamidae.
The ordination diagram in Figure 3 showed that environmental variables significantly explained benthic microalgae assemblage. Certain benthic microalgae genera either increased or decreased their abundance in response to different environmental variables. Redundancy analysis (RDA) of benthic microalgae genera with 10 environmental variables of interest showed two axes that are predictors of water quality. The first axis was associated with autochthonous inputs of the river as indicated by the arrows of BOD and pH in the ordination diagram. The second axis revealed the importance of nitrite, Total Dissolved Solids (TDS), and temperature as the influencing variables. These variables are related to geologic and allochthonous inputs. In this study, the genera that favoured high concentrations of BOD (axis 1) were at the right quadrant of the diagram whereas those adapted to poor water quality such as low pH (axis 1), high temperature, low nitrite concentrations and high concentration of TDS (axis 2) are at the left quadrant.
The percentage variance that explained the relationship between macroinvertebrates and the environmental variables (46.6% in axis 1 and 61.3% in axis 2) was not as high as that of the benthic microalgae (47.6% in axis 1 and 73.3% in axis 2). This indicated that the number of macroinvertebrate individuals counted was not as robust as the standard counts. Results showed that only pH and nitrate are good predictors of the macroinvertebrate assemblage. Among the physical and chemical variables measured, only pH, TDS, nitrite, BOD and temperature significantly explained the variation of benthic microalgae assemblage. Certain genera of microalgae can be used as an indicator of low pH and nitrite, high TDS, temperature and BOD of the river or as bioindicators of water quality. Worldwide, diatoms are being used in monitoring the quality of rivers, but in the Philippines this is still not widely used. Currently, freshwater biomonitoring and assessment is being increasingly implemented in Southeast Asian countries such as China . In running waters, where changes in hydrology are rapid and difficult to estimate, biological monitoring has proven to be very useful due to its integrating nature .
This study proved that certain families of macroinvertebrates and genera of benthic microalgae responded to riverine environmental variables in a tropical river. The families of Perlodidae, Dipseudopsidae and Ephemerellidae preferred higher pH while Lymnaedae, Planorbidae, Viviparidae, Pilidae and Philopotamidae thrived in increasing nitrate concentration. Benthic microalgae genera such as Rhopalodia, Epithemia and Melosira favored high oxygen demand. Pinnularia, Gyrosigma and Gomphonema required low pH. Surirella, Cymbella, Achnanthes and Fragilaria thrived in low nitrite and low total dissolved solids concentration. Dissolved oxygen (DO), phosphate, nitrate and silicate did not influence the biota studied. However, biological assemblage can be specific for certain environmental variable and may provide clues of the prevailing physical and chemical conditions of the river.
Received 3 October 2015
Accepted 10 October 2015
Published Online 13 November 2015
The authors would like to thank the Kinaadman Research Center (KRC), Xavier University-Ateneo de Cagayan for funding the project. Dr. Hilly Ann Roa-Quiaoit and Fr. Mars P. Tan, SJ the Ridge to Reef (R3) project leaders for trusting our team (XUFBioT) to do the biology component and some physico-chemical parameters. Dr. Ester L. Raagas for generating statistical outputs and guidance on interpretation of statistical results. Engr. Dexter S. Lo and the Engineering Resource Center (XU-ERC) for providing us with GIS map of the study area.
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(1,2) Astrid L. Sinco, (1) Leolinda L. Saab, (1) Judy P. Sendaydiego, (1) Geraldine R. Mojica, (1) Giovanni G. Tampus and (1) Adora S. Rondez
(1) Biology Department, Xavier University, Cagayan de Oro City, Philippines
(2) Kinaadman Research Center, Xavier University, Cagayan de Oro City, Philippines
Corresponding Author: Astrid L., Biology Department, Xavier University, Cagayan de Oro City, Philippines
Tel: +639178136262; E-mail:email@example.com
Table 1: Macroinvertebrate assemblage and % relative abundance in the main channel and tributaries of Cagayan de Oro River, Philippines Taxon Relative Abundance (%) Main Channel Tributaries Pulmonata Lymnaedae 0.24 Physidae - 0.45 Pilidae 0.97 Planorbidae 0.12 Viviparidae 0.36 Veneroida Sphaeriidae 0.12 Plecoptera Perlidae 0.15 0.76 Perlodidae - 0.18 Leuctridae - 0.07 Trichoptera Calamoceratidae - 0.15 Brachycentridae 0.12 Dipseudopsidae 2.05 2.64 Glossomatidae 0.27 0.27 Hydroptilidae 1.40 3.30 Hydropsichidae 1.72 1.78 Limnephilidae - 0.35 Philopotamidae 1.47 0.67 Psychomyiidae 0.41 0.95 Rhyacophilidae 0.15 Phryganeidae 0.15 Ephemeroptera Baeticidae 22.46 15.57 Baetidae 9.78 11.02 Caenidae 2.32 2.73 Ephemerellidae 0.95 2.82 Heptageniidae 1.14 1.89 Leptophlebiidae - 1.60 Neoephemeridae 0.87 1.28 Oligoneuridae 1.45 0.07 Polymitarcyidae 0.12 Potamanthidae - 0.45 Prossopistomatidae - 0.30 Teloganeliidae - 0.32 Trychorythidae 9.19 4.14 Family Relative Abundance (%) Main Channel Tributaries Coleoptera Carabidae 0.12 Dysticidae - 0.26 Elmidae 4.44 4.52 Gyrinidae 0.12 Haliplidae 0.12 0.07 Noteridae 0.60 Psephenidae 3.69 1.65 Ptilodactylidae - 0.09 Diptera Dixidae 0.90 2.15 Empididae 0.12 0.09 Ceratopogonidae 0.24 Simuliidae 2.88 2.23 Tipulidae 0.58 3.19 Chironomidae 22.94 26.54 Culiicidae - 0.27 Hemiptera Belostomatidae - 0.07 Gerridae 0.97 1.83 Nepidae - 0.07 Pleidae 0.36 Veliidae 0.95 0.77 Lepidoptera Crambidae 0.15 0.41 Pyralidae 0.27 0.51 Megaloptera Sialidae - 0.15 Odonata Corduliidae - 0.09 Libelluidae 0.12 Calopterygidae 0.12 0.35 Protoneuridae - 0.18 Nematomorphan family - 0.07 Oligochaete family 0.48 0.39 Turbellaria family 0.60 0.07 Unidentified pupa 1.24 0.18 Table 2: Mean cell density (cells/[in.sup.2]) of benthic microalgae in Cagayan de Oro River. Taxa Main Channel Tributary Chlorophyceae Ankistrodesmus - 0.04 Cladophora - 0.001 Cosmarium 0.17 0.05 Nostoc 1.99 0.15 Pediastrum 0.01 Scenedesmus 0.15 0.15 Euglenophyceae Euglena 0.05 0.06 Phacus 0.05 0.01 Xanthophyceae Tribonema - 0.02 Bacillariophyceae Achnanthes 0.81 0.95 Amphora 0.04 0.29 Cocconeis 2.48 1.26 Coscinodiscus - 0.001 Cyclotella 0.11 0.10 Cymbella 1.95 3.19 Diadismes - 0.03 Epithemia 0.35 0.49 Fragilaria 3.95 7.32 Gomphonema 5.25 5.14 Gyrosigma 0.64 0.49 Melosira 1.43 2.37 Navicula 17.92 19.66 Nitzschia 0.77 0.55 Pinnularia 1.98 1.33 Rhizosolenia - 0.01 Rhopalodia 0.21 0.13 Surirella 0.84 1.17 Synedra 0.35 0.35 Cyanophyceae Oscillatoria 10.24 5.28 Spirulina - 0.01 Table 3: Physical and chemical variables measured in the nine sites from the main channel and eight sites from the tributaries of Cagayan de Oro River. Indicators of River's Standard Main Channel Physical and Chemical Values Mean value Conditions Total suspended solids 50mg/L 67.10 Total dissolved solids 1000 mg/L 175.21 Dissolved oxygen 5 mg/L 6.83 Conductivity 600ms 345.22 Orthophosphate Class A: 0.1mg/L 0.15 Nitrate 10mg/L 0.31 Nitrite 0.1mg/L 0.02 Silicate 1-100 89.30 pH 6.5 - 8.5 8.12 Temperature 26[degrees]C - 27.19 Velocity (m/s) 30[degrees]C 0.13 Indicators of River's Tributaries Minimum & Physical and Chemical Mean value Maximum values Conditions Total suspended solids 26.45 3.00 - 339.04 Total dissolved solids 127.68 43.67 - 511.50 Dissolved oxygen 6.04 5.27 - 9.17 Conductivity 240.98 82.57 - 1103.83 Orthophosphate 0.11 0.03 - 0.65 Nitrate 0.25 0.02 - 1.05 Nitrite 0.02 0.01 - 0.03 Silicate 104.78 23.80 - 226.67 pH 8.23 7.15 - 8.89 Temperature 25.77 21.54 - 29.61 Velocity (m/s) 0.18 0.06 - 0.35 Table 4: Summary statistics on the relationship between biological and physico-chemical variables using redundancy analysis and Monte Carlo permutation test. Only environmental variables selected in the forward selection procedure are presented in the ordination (** highly significant at a < 0.01). Biological Variables Axis 1 Axis 2 F value Families of macroinvertebrates Correlation between 0.96 0.70 macroinvertebrates and environmental variables Percentage of variance of macroinvertebrates explained 20.8 27.3 in axes 1 & 2 Percentage of variance of macroinvertebrates-environment 46.6 61.3 1.69 ** relation Weighted correlation of environmental variables with macroinvertebrates Nitrate 0.03 -0.06 pH -0.46 0.10 Genera of benthic microalgae Correlation between benthic 0.91 0.87 microalgae and environmental 28.0 43.2 variables Percentage of variance of benthic microalgae explained in axes 1 & 2 Percentage of variance of benthic microalgae-environment relation 47.6 73.3 Weighted correlation of environmental variables with benthic microalgae pH -0.87 0.19 3.01 ** TDS -0.25 0.60 BOD 0.45 0.37 Nitrite -0.27 -0.28 Temperature 0.05 0.61
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|Author:||Sinco, Astrid L.; Saab, Leolinda L.; Sendaydiego, Judy P.; Mojica, Geraldine R.; Tampus, Giovanni G.|
|Publication:||Advances in Environmental Biology|
|Date:||Oct 1, 2015|
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