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Phenylethylamine can be used for prevention and treatment of bacterial biofilms.

Previous research in our lab has found PEA to be one of the two most effective of 95 carbon and 95 nitrogen sources screened for their effect on Escherichia coli O157:H7 growth, bacterial counts, and biofilm amounts on treated pieces of beef (Lynnes et al., 2013). A range of PEA concentrations (0 to 100 mg/ml) was used to test the effectiveness of PEA on pathogens that form bacterial biofilms in the food industry and clinical settings. In TSB at 37[degrees]C, PEA was found to be more effective at reducing growth and biofilm amounts of the gram negative pathogens, in comparison to the one gram positive pathogen. In a second experiment, different environmental factors, such as time of incubation, temperature, and growth medium were modified to see how the environment impacts the effect of PEA. In general, the pathogens grown in more nutrient rich media had higher growth and biofilm amounts, but the higher temperature was more effective reducing growth and biofilm amounts. The addition of PEA at different time points after Elocution was tested in a third experiment, using an E. coli K-12 strain. PEA was found to reduce both growth and biofilm amounts when added after long time after the initial inoculation. This leads us to believe that PEA could be used as a preventative measure and treatment of bacterial biofilms in both the food processing industry and clinical setting.

The research was funded by grant 1R15AI089403 from the NIH/NIAID and the North Dakota Agricultural Products and Utilization Commission (APUC).

Lynnes T, Horne SM & PruE BM (2013) E-phenylethylamine as a novel nutrient treatment to reduce bacterial contamination due to Escherichia coli O157:H7 on beef meat. Meat Sci 96:165-171.

Meredith Irsfeld [1] *, Shelley M. Home [1], Shane J. Stafslien [2], Birgit M. Pruss [1]

[1] Department of Veterinary and Microbiological Sciences, North Dakota State University. Fargo, ND 58105

[2] Center for Nanoscale Science and Engineering, North Dakota State University, Fargo, ND 58105 Graduate Student, Supervisor Birgit Pruss

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Title Annotation:Graduate Communications IN THE A. RODGER DENISON COMPETITION
Author:Irsfeld, Meredith; Home, Shelley M.; Stafslien, Shane J.; Pruss, Birgit M.
Publication:Proceedings of the North Dakota Academy of Science
Article Type:Author abstract
Date:Apr 1, 2015
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