Pathogenic pseudorabies virus, China, 2012.
Vaccination of pigs with attenuated live or inactivated vaccines is widely performed to reduce the huge economic losses caused by PRV infection (10-12). Although vaccination confers protection against disease, it does not prevent infection from a wild-type strain. Thus, both the virus in the vaccine and the super-virulent wild-type strain can establish latency within the same animal (13-15).
We report an outbreak of PRV infection that devastated the swine-producing regions of China in 2012. We systematically investigated the outbreak to identify the causative agent.
In January 2012, a previously unknown severe disease was observed in pigs on several farms in northern and eastern China. In Shandong Province, > 80,000 pigs were infected. The affected pigs had high fever ([greater than or equal to40.5[degrees]C), anorexia, coughing, respiratory distress, conjunctival serous and mucinous secretion, and posterior paralysis. The disease was first observed in older pigs and spread within 2-3 days to younger pigs. Duration of disease was 5-7 days. Rate of illness reached 50%, and mortality was 3%-5%. Most pig deaths were recorded on the third day after monitoring began. Abortion was observed in [approximately equal to]35% of sows that were 70-90 days pregnant. Viscera (e.g., lung, kidney, heart, liver, and spleen) and serum samples were collected from dead pigs from different provinces. Pathologic examination showed the most striking gross lesions were consolidated in the lungs (Figure 1, panel A), with edema and hemorrhage (Figure 1, panel B). In addition, foci of yellow-white necrosis were observed in the kidneys of some dead pigs (Figure 1, panel C).
To gain insight into the etiologic agent of the disease, we conducted extensive and systematic diagnostic testing, including PCR, ELISA, viral isolation, immunohistochemical staining, and standard bacteriologic culture, to evaluate the specimens. Marc-145 cells, inoculated with various tissue homogenates, showed cytopathic effects. A specific PRV monoclonal antibody was used, and immunopositive cells were observed in infected tissue (online Technical Appendix Figure 1, panels A, B, wwwnc.cdc.gov/ EID/article/20/1/13-0531-Techapp1.pdf). The PCR for inocula samples showed that many glycoprotein (g) genes of PRV could be amplified by using the primers specific to the unique gene fragments (online Technical Appendix Figure 2). The PRV gE-ELISA assays (IDEXX Laboratories, Westbrook, ME, USA) indicated that serum samples from the sick pigs contained antibodies against wild-type, virulent PRV glycoprotein E but not against the vaccine strain (Table). All these results indicated that PRV was the causative agent of this disease. Our results also ruled out other suspected agents, such as classical swine fever, African swine fever, porcine reproductive and respiratory syndrome virus, and some bacterial infections. The 3 isolates found here are referred to as NVDC-PRV-BJ, NVDCPRV-HEB, and NVDC-PRV-SD, according to the provinces from which they were isolated.
The pigs vaccinated with attenuated live PRV vaccines still showed clinical signs of PRV during the outbreak. To confirm the presence of PRV in these herds, 15 pigs were vaccinated with the current vaccine strain and then challenged with the NVDC-PRV-SD strain 21 days after vaccination. The results indicated that the vaccinated pigs had not been given completely effective protection against infection and exhibited obvious clinical signs of disease similar to the typical symptoms observed in the field, suggesting that the virulence of the newly isolated PRV strains had changed. This virulence may have caused the deaths of infected pigs.
To better understand the genetic relationship of the 3 PRV isolates found here to other PRV isolates, we amplified and sequenced the 15 major genes of the 3 isolates (online Technical Appendix Table 1). Compared with other PRV isolates (online Technical Appendix Table 2), there was a 21-nt insertion from nucleotide positions 185-205 in the gC gene, similar to SA215, BJ, DG, Ea, Fa, and P-PrV strains (Figure 2, panel A), which shared 100% nt identity with each other. The gD gene, like isolates Ea, Fa, SA215, and Yangsan, had 6 nt at positions 801-806 and shared 99.4%-99.8% identity with each other (Figure 2, panel B). There were 2 insertions of 6 discontinuous nucleotides each at positions 138-140 and 1472-1474 in the gE gene (Figure 2, panel C). These had 99.9%-100% identity with each other. The rest of the genes from the 3 isolates had no nucleotide insertions or deletion in common with the other PRV isolates. We further analyzed the relationship of these 3 isolates with other PRV isolates using a phylogenetic tree based on the gE gene; the 3 isolates formed a tightly clustered branch and were very closely related to other isolates from Asia (online Technical Appendix Figure 3).
We describe and analyzed a major outbreak of PRV in pigs in China. In these herds, all pigs had been vaccinated against PRV 3 times a year, at approximately the same time as each other. The disease spread to > 6 provinces (including autonomous cities and regions) and caused considerable economic losses among local pig farms.
PRV has been recognized as a source of infection for pigs and continues to circulate globally among herds (9). In particular, pigs receiving attenuated live PRV vaccines showed typical clinical symptoms, which suggest that these isolates might have evolved new types of pathogenicity.
Even though the PRV isolates showed nucleotide insertions in the gC, gD, and gE genes, the molecular mechanisms underlying their high pathogenesis have yet to be elucidated. The origin of these lethal isolates within China is still obscure, although phylogenetic trees based on the gE gene here indicated that the 3 isolates are more closely related to the Asia PRV isolates, especially the China isolates, than to isolates from other countries. Because the virulence and origin of PRV is thought to be associated with multiple factors, whether such insertions are related to the virulence of PRV remains an issue and requires further investigation.
In summary, our study indicates that an outbreak of disease in pigs in China, which was of unprecedented scale, was caused by PRV infection. Other pathogens were ruled out. Our findings highlight the need to prevent and control the spread of this virus.
This work was supported by grants from the National Science and Technology Pillar Program in the 11th 5-year plan period of China (2006BAD06A07, 2009BADB4B05), National Basic Research Program of China (2008FY130100-2), and Scientific Achievement Transformation Program (2009GB23260435).
Dr Yu is an epidemiologist and pathologist at Veternary Diagnosis Center and OIE Porcine Reproductive and Respiratory Syndrome Laboratory, China Animal Disease Control Center. Her research interests are epidemiology and pathologic analyses of infectious disease.
(1.) Klupp BG, Hengartner CJ, Mettenleiter TC, Enquist LW. Complete, annotated sequence of the pseudorabies virus genome. J Virol. 2004;78:424-40. http://dx.doi.org/10.1128/JVI.78.L424-440.2004
(2.) Pomeranz LE, Reynolds AE, Hengartner CJ. Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev. 2005;69:462-500. http://dx.doi. org/10.1128/MMBR.69.3.462-500.2005
(3.) Mettenleiter TC, Keil GM, Fuchs W. Molecular biology of animal herpesviruses. In: Mettenleiter TC, Sobrino F, editors. Animal viruses: molecular biology. Norfolk, UK: Caister Academic Press; 2008. p. 531.
(4.) Szpara ML, Tafuri YR, Parsons L, Shamim SR, Verstrepen KJ, Legendre M, et al. A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses. PLoS Pathog. 2011;7:e1002282. http://dx.doi.org/10.1371/journal.ppat.1002282
(5.) Fonseca AA Jr, Camargos MF, de Oliveira AM, Ciacci-Zanella JR, Patricio MA, Braga AC, et al. Molecular epidemiology of Brazilian pseudorabies viral isolates. Vet Microbiol. 2010;141:23 8-45. http://dx.doi.org/10.1016/j.vetmic.2009.09.018
(6.) Marcaccini A, Lopez Pena M, Quiroga MI, Bermudez R, Nieto JM, Aleman N. Pseudorabies virus infection in mink: a hostspecific pathogenesis. Vet Immunol Immunopathol. 2008;124:264-73. http://dx.doi.org/10.1016/j.vetimm.2008.03.013
(7.) Pensaert MB, Kluge JP. Pseudorabies virus (Aujeszky's disease). In: Pensaert MB, editor. Virus infection of porcines. Amsterdam: Elsevier Science Publishers; 1989. p. 39-65.
(8.) Wittmann G, Rziha HJ. Aujeszky's disease (pseudorabies) in pigs. In: Wittmann G, editor. Herpesvirus diseases of cattle, horses and pigs. London: Kluwer Academic Publishers; 1989. p. 230-325.
(9.) Wittmann G, Rziha H. Aujeszky's disease (pseudorabies) in pigs. Developments in Veterinary Virology (USA). 1989;9:230-325.
(10.) van Rooij EM, Moonen-Leusen HW, de Visser YE, Middel WG, Boersma WJ, Bianchi AT. A DNA vaccine coding for gB and gD of pseudorabies virus (suid herpes type 1) primes the immune system in the presence of maternal immunity more efficiently than conventionalvaccines. Vaccine. 2006;24:1264-73. http://dx.doi. org/10.1016/j.vaccine.2005.09.041
(11.) Mettenleiter TC, Klupp BG, Weiland F, Visser N. Characterization of a quadruple glycoprotein-deleted pseudorabies virus mutant for use as a biologically safe live virus vaccine. J Gen Virol. 1994;75:1723-33. http://dx.doi.org/10.1099/0022-1317-75-7-1723
(12.) Cunha EMS, Peixoto ZMP, Kotait I, Bersano JG. Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs. Brazilian Journal of Veterinary Research and Animal Science. 1998;35:188-94 [cited 2013 Nov 12]. http://www.scielo.br/ pdf/bjvras/v35n4/35n4a09.pdf
(13.) Lipowski A. Evaluation of efficacy and safety of Aujeszky's disease vaccines. Pol J Vet Sci. 2006;9:75-9.
(14.) Wittmann G. Spread and control of Aujeszky's disease (AD). Comp Immunol Microbiol Infect Dis. 1991;14:165-73. http://dx.doi. org/10.1016/0147-9571(91)90129-2
(15.) Puentes E, EirasA, Cancio E, Nores MV,AguileraA, et al. Comparison of the protective efficacy of Aujeszky's disease (pseudorabies) virus glycoproteins obtained from different sources. Vet Microbiol. 1993;35:45-59. http://dx.doi.org/10.1016/0378-1135(93)90115-N
Xiuling Yu,  Zhi Zhou, (1) Dongmei Hu,  Qian Zhang,  Tao Han, Xiaoxia Li, Xiaoxue Gu, Lin Yuan, Shuo Zhang, Baoyue Wang, Ping Qu, Jinhua Liu, Xinyan Zhai, and Kegong Tian
 These authors contributed equally to this article.
Author affiliations: China Animal Disease Control Center, Beijing, China (X. Yu, Z. Zhou, D. Hu, Q. Zhang, T. Han, X. Li, X. Gu, L. Yuan, S. Zhang, B. Wang, P. Qu, X. Zhai, K. Tian); China Agricultural University, Beijing (D. Hu, Q. Zhang, J. Liu); and National Research Center for Veterinary Medicine, Luoyang, Henan, China (K.Tian)
Address for correspondence: Kegong Tian, Veternary Diagnosis Center and OIE Porcine Reproductive and Respiratory Syndrome Laboratory, China Animal Disease Control Center, No. 20 Maizidian Rd., Chaoyang District, Beijing, 100125, China; email: email@example.com
Table. Antibodies to PRV gE in serum from PRV-infected pigs from different provinces, China, 2012 Sample origin province Collection date gE ELISA S/N ratio * Shandong 2012 Jan 0.115 Beijing 2012 Feb 0.240 Hebei 2012 Feb 0.169 Tianjin 2012 Feb 0.171 Liaoning 2012 Mar 0.168 0.274 0.168 0.173 0.177 0.179 0.168 0.158 0.143 * S/N ratio was calculated as a ratio of the absorbance of a well with serum to the absorbance of a control well without serum. Serum with an S/N ratio of [less than or equal to]0.60 was considered positive. PRV, pseudorabies virus; g, glycoprotein.
|Printer friendly Cite/link Email Feedback|
|Author:||Yu, Xiuling; Zhou, Zhi; Hu, Dongmei; Zhang, Qian; Han, Tao; Li, Xiaoxia; Gu, Xiaoxue; Yuan, Lin; Zha|
|Publication:||Emerging Infectious Diseases|
|Date:||Jan 1, 2014|
|Previous Article:||Rapidly fatal hemorrhagic pneumonia and group A Streptococcus serotype M1.|
|Next Article:||Novel avian coronavirus and fulminating disease in guinea fowl, France.|